Sed0005688 (gene) Chayote v1

Overview
NameSed0005688
Typegene
OrganismSechium edule (Chayote v1)
DescriptionRNA pseudouridine synthase 1
LocationLG03: 13601111 .. 13604086 (-)
RNA-Seq ExpressionSed0005688
SyntenySed0005688
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCACAAAATGGCCCTCTTCGTCGCCCTTCAACTCTTCTCATTTCCCCACAAACCCTTCACCAAATTCCCAAAACCCTTTTGTTTCCGAACAATCGAACCGATCGGCAGCAGCATGGCAGAGGCGGAGCCATCAAAGCCGGTGGCCGGAAACAGCCCAGCAACCGGAGAAAACTACCCGGTGCCTCTCTCGCCGCCATTGCCGGCCATATCCAAGAACTTGGAGCTCGCTAGAGCCATGGCCGCCTCCTCTAAGTCCAGTCTCTTTTCTTTATCCGCAAGCGATGTTATATACGAGGACGAGTGGCTCATTGTCGTCAATAAGCCGCAAGGAGTCTATTGTGAAAGCGTTTTGGCCTCTGCTCATCGTTTTCTCGGTGATTCCGCCATGGCTAGTCTGCAGAGTGGAGGTACTAGAATCTCTCTGTTTATGTTGGCTCTTTGTTTTGCTCATGAGTTTTCCAATATGTGCAATTTGTTCGTGTTTGGAGATGCTACTCTTAGGATTTTTTTTTTTTTGAGACATTCAGATTTTAATATGAATTGAATTTTAACCTAGATTTTTGTGTTTGATAATTGGATGGAGATGGTTCAAGTGCAATTCTGAAGTTTCTTTGGTTTTGTAAAAAAGTTTTTTTCTAGTGTTTGACCAGCAAACTCGATTACTGTCACGGAAGATAGAATCTGACCCTACAACATGTAGATGTCAAAGAAATTTGTATGGAGTTAATTTTTAAGTAGGTGACCAACATAAAATGAATTCGATGATGGTGGTTGTTTTGTATAAAGGTATTAGCATATACTTTCAATGGATATATCTAGGGCTGAATTTTTTTGTTAAAATAGTTTGGAGAGATTTATTCCATATAAGTCTTGAGCATTTATTTTTATAGATCACACTTTTGTGCATTGATATTTAACTTGGATTTTGTCGAAATTTAGCCGAAGTTAGTGAATAAATGTAAACAAAACTTGTTTTCGTCTTCGGAAATTGACTTTGAATTCAAATCTTTTGTTAATAAAGATGAAACCCACAGGAAACAAACACAGTTTTTAAAAATAATTAAAAAGCTAAATGTTATCAAAGGGAGCCTCAAGTTTTCTTTTCTTTTTTCCTTACTGAAAGTGAAACACCTCGTCAAATGGTTTTTAAAGAGAGACAAAAAATCCATCTTTAGTTTTTAGCTAAGTTATCAAAGAATCCTTGTGGTTATGTCATTAAATTATGTAATGTTCCTAAGTGGTAGCACGATGGTCGAAGGCTTGGGCTTTGAAGGTATGCTCTTCTCAAGGTTTGAGGTTCGATATTCACCTGTGACATTAATTTGAAGATACCTCTCATGTCTCCATTAATGTCTCCCGGTGCTTGGCCTAAGATGGGTGCAATTATCCTGGGTATAGTGAAGCAAAACTCCAATTTTCCGGTTTCAAAAAAAAAAATTATGTAATAATGTTTTTTGGGGTGCTGTGTGATATAAATACTATGATAGTTGGGCAGGCTCTGGGAGTTTGATACGAGTACATTTAAATTGAGTAATTGACTTGTGCTGCTCTCCCTACATTGCCAAAATGGCAATGCTTCTGATCATTAGACAGCTGATATAAATGGTTTTTTTTAGCTATATCCTGGTTGTTGAAGAGAGAGTTGCTTGGATGTGTCTGAAGTGCTAAACTTTTGTCTAAAGTGTTGTCATGTAATTACATCTTTTGATTATCTATGACTATCAATTTATCAGATTGTTCCCTGCTTGGATTCTTCATATGCAGAGATCAAGAACAGTTTTCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACCAGTGGGGTGATGGTTATAACTAAGTCGCATAAAGTCGCTTCTAAATTAGTGAAGGCGTTTACTGACCACAAGGTTTCAAAATCATATATTGCCTTCTGCGTTGGCTCTGCTCCAAAATGGAAAAAAATCAACATCAAATCTGGTCATGGAAGGTCAAAGTTCGGGGTATGGCGTGTTTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCGTAGTCAGAGACATGGAAACCCATTTTGAAGTATTGTCAGTAAATGGACAAAGAAGCACCATGGAGGAACTTCCAGACTCTAGTAAAGATCAAGAAGAAACAATTGTAGTCCAATCGAAATCTCTGATAGATGTTGACGCTCAGAAGCACGAGATGCTGATTCGAGCACGACCTCGAAGTGGACGGACTCATCAAATCCGTCTGCATTGCCAATATCTCGGAACTCCCATCGTAGGAGATGTGAAATACGAGGGTGTTACAGAGTGGAATGGAAATTTTTTTGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTGTTACAGGTATTCCTCTCAAACTGCAGGCTCCTCTACCATTATGGGCCAGCCAAGCATGGCAGCCCTCGCAACAAGAGGTGAACAATATCATTGGTACAGTTGAGTGAGAACCGAATATAACTTTCGTTTTACAAAGAGCCTCAGCGTCGAAGAATGTTGCTAAAGCAAATGAGGTGATATTCAATCTAATTCGTACAAGTCGAACTACAGAGTTCTTGCATCATTGGCTTTCTGTCTGCATTCTTCCTTGATATGTATCTCCTCATTGAAGCAGAGATTTGAATGAGCTCTTGTGACCGCAACAGTCTCGAACAAAAATATCTACAGAGTCGGAATCGGAAATCAAACAACTTGCTAAGTTTCGTCTGGAATTTTGAGAGGGTGGCAAATGGAAGAAGAAGAAGAAGCATGTATGTCTAAGGCGTACGAGTTTGTGCAAAGCCCTTTGGAAATGCATCTTCTAGAGCCATTTATTGATAGAATTCATGTACAATCCTGAAGAAAAATGTTGAGAATGAAAAAAATGTATGGTTTAAATTATTTTTTTTTAATTAATTATGCATATACTATTTATTGATTGAAGTTTCAAATAGTAAATAATGAAGTAC

mRNA sequence

GTCACAAAATGGCCCTCTTCGTCGCCCTTCAACTCTTCTCATTTCCCCACAAACCCTTCACCAAATTCCCAAAACCCTTTTGTTTCCGAACAATCGAACCGATCGGCAGCAGCATGGCAGAGGCGGAGCCATCAAAGCCGGTGGCCGGAAACAGCCCAGCAACCGGAGAAAACTACCCGGTGCCTCTCTCGCCGCCATTGCCGGCCATATCCAAGAACTTGGAGCTCGCTAGAGCCATGGCCGCCTCCTCTAAGTCCAGTCTCTTTTCTTTATCCGCAAGCGATGTTATATACGAGGACGAGTGGCTCATTGTCGTCAATAAGCCGCAAGGAGTCTATTGTGAAAGCGTTTTGGCCTCTGCTCATCGTTTTCTCGGTGATTCCGCCATGGCTAGTCTGCAGAGTGGAGAGATCAAGAACAGTTTTCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACCAGTGGGGTGATGGTTATAACTAAGTCGCATAAAGTCGCTTCTAAATTAGTGAAGGCGTTTACTGACCACAAGGTTTCAAAATCATATATTGCCTTCTGCGTTGGCTCTGCTCCAAAATGGAAAAAAATCAACATCAAATCTGGTCATGGAAGGTCAAAGTTCGGGGTATGGCGTGTTTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCGTAGTCAGAGACATGGAAACCCATTTTGAAGTATTGTCAGTAAATGGACAAAGAAGCACCATGGAGGAACTTCCAGACTCTAGTAAAGATCAAGAAGAAACAATTGTAGTCCAATCGAAATCTCTGATAGATGTTGACGCTCAGAAGCACGAGATGCTGATTCGAGCACGACCTCGAAGTGGACGGACTCATCAAATCCGTCTGCATTGCCAATATCTCGGAACTCCCATCGTAGGAGATGTGAAATACGAGGGTGTTACAGAGTGGAATGGAAATTTTTTTGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTGTTACAGGTATTCCTCTCAAACTGCAGGCTCCTCTACCATTATGGGCCAGCCAAGCATGGCAGCCCTCGCAACAAGAGGTGAACAATATCATTGGTACAGTTGAGTGAGAACCGAATATAACTTTCGTTTTACAAAGAGCCTCAGCGTCGAAGAATGTTGCTAAAGCAAATGAGGTGATATTCAATCTAATTCGTACAAGTCGAACTACAGAGTTCTTGCATCATTGGCTTTCTGTCTGCATTCTTCCTTGATATGTATCTCCTCATTGAAGCAGAGATTTGAATGAGCTCTTGTGACCGCAACAGTCTCGAACAAAAATATCTACAGAGTCGGAATCGGAAATCAAACAACTTGCTAAGTTTCGTCTGGAATTTTGAGAGGGTGGCAAATGGAAGAAGAAGAAGAAGCATGTATGTCTAAGGCGTACGAGTTTGTGCAAAGCCCTTTGGAAATGCATCTTCTAGAGCCATTTATTGATAGAATTCATGTACAATCCTGAAGAAAAATGTTGAGAATGAAAAAAATGTATGGTTTAAATTATTTTTTTTTAATTAATTATGCATATACTATTTATTGATTGAAGTTTCAAATAGTAAATAATGAAGTAC

Coding sequence (CDS)

ATGGCCCTCTTCGTCGCCCTTCAACTCTTCTCATTTCCCCACAAACCCTTCACCAAATTCCCAAAACCCTTTTGTTTCCGAACAATCGAACCGATCGGCAGCAGCATGGCAGAGGCGGAGCCATCAAAGCCGGTGGCCGGAAACAGCCCAGCAACCGGAGAAAACTACCCGGTGCCTCTCTCGCCGCCATTGCCGGCCATATCCAAGAACTTGGAGCTCGCTAGAGCCATGGCCGCCTCCTCTAAGTCCAGTCTCTTTTCTTTATCCGCAAGCGATGTTATATACGAGGACGAGTGGCTCATTGTCGTCAATAAGCCGCAAGGAGTCTATTGTGAAAGCGTTTTGGCCTCTGCTCATCGTTTTCTCGGTGATTCCGCCATGGCTAGTCTGCAGAGTGGAGAGATCAAGAACAGTTTTCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACCAGTGGGGTGATGGTTATAACTAAGTCGCATAAAGTCGCTTCTAAATTAGTGAAGGCGTTTACTGACCACAAGGTTTCAAAATCATATATTGCCTTCTGCGTTGGCTCTGCTCCAAAATGGAAAAAAATCAACATCAAATCTGGTCATGGAAGGTCAAAGTTCGGGGTATGGCGTGTTTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCGTAGTCAGAGACATGGAAACCCATTTTGAAGTATTGTCAGTAAATGGACAAAGAAGCACCATGGAGGAACTTCCAGACTCTAGTAAAGATCAAGAAGAAACAATTGTAGTCCAATCGAAATCTCTGATAGATGTTGACGCTCAGAAGCACGAGATGCTGATTCGAGCACGACCTCGAAGTGGACGGACTCATCAAATCCGTCTGCATTGCCAATATCTCGGAACTCCCATCGTAGGAGATGTGAAATACGAGGGTGTTACAGAGTGGAATGGAAATTTTTTTGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTGTTACAGGTATTCCTCTCAAACTGCAGGCTCCTCTACCATTATGGGCCAGCCAAGCATGGCAGCCCTCGCAACAAGAGGTGAACAATATCATTGGTACAGTTGAGTGA

Protein sequence

MALFVALQLFSFPHKPFTKFPKPFCFRTIEPIGSSMAEAEPSKPVAGNSPATGENYPVPLSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDEWLIVVNKPQGVYCESVLASAHRFLGDSAMASLQSGEIKNSFPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETHFEVLSVNGQRSTMEELPDSSKDQEETIVVQSKSLIDVDAQKHEMLIRARPRSGRTHQIRLHCQYLGTPIVGDVKYEGVTEWNGNFFDSHELHAESLYFVHPVTGIPLKLQAPLPLWASQAWQPSQQEVNNIIGTVE
Homology
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BLAST of Sed0005688 vs. NCBI nr
Match: KAG7021056.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 580.9 bits (1496), Expect = 7.9e-162
Identity = 305/368 (82.88%), Postives = 320/368 (86.96%), Query Frame = 0 

Query: 1   MALFVALQ-LFSFPHKPFTKFPKPFCFRTIEPIGSSMAEAEPSKPVAGNSPATGENYPVP 60
           MAL V +Q  FS PHK F KFPKPF F    P+ +SMA++  SKPV      TGENYPVP
Sbjct: 6   MALVVTIQPPFSIPHKSFAKFPKPFRFHRFRPVTNSMADS--SKPV------TGENYPVP 65

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDEWLIVVNKPQGVYCESVLASAH 120
           LSPPLPAISKNLELARAMAA SKSSLFSLSA DVIYEDEWLI VNKPQGVYCESVLAS  
Sbjct: 66  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKPQGVYCESVLASVP 125

Query: 121 RFLGDSAMASLQSGEIKNSFPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 180
           RFLGDSAMA  QSGEIK S PELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK
Sbjct: 126 RFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 185

Query: 181 SYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETHFEVLSV 240
           SYIAFCVGSAPKWKKI +KSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET FEVLSV
Sbjct: 186 SYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSV 245

Query: 241 NGQRSTMEELPDSSKD----QEETIVVQSKSLIDVDAQKHEMLIRARPRSGRTHQIRLHC 300
           NGQ +T EELP+S KD    +EETIV QSKSLID+DA+K E+LIRARPRSGRTHQIRLH 
Sbjct: 246 NGQ-NTKEELPESRKDEEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHS 305

Query: 301 QYLGTPIVGDVKYEGVTEWNGNFFDSHELHAESLYFVHPVTGIPLKLQAPLPLWASQAWQ 360
           QYLG PIVGDVKYEGVTEWNG  +DSHELHAESLYFVHPVTGIPLKLQAPLP WA+QA Q
Sbjct: 306 QYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQALQ 364

Query: 361 PSQQEVNN 364
           P QQEVN+
Sbjct: 366 PQQQEVNS 364

[Close]
BLAST of Sed0005688 vs. NCBI nr
Match: KAG6586224.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 574.7 bits (1480), Expect = 5.7e-160
Identity = 303/370 (81.89%), Postives = 319/370 (86.22%), Query Frame = 0 

Query: 1   MALFVALQL-FSFPHKPFTKFPKPFCFRTIEPIGSSMAEAEPSKPVAGNSPATGENYPVP 60
           MAL V LQ  FS P+K F KFPKPF F    P+ +SMA++  SKPV      TGENYPVP
Sbjct: 1   MALVVTLQAPFSIPNKSFAKFPKPFRFHRFRPVTNSMADS--SKPV------TGENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDEWLIVVNKPQGVYCESVLASAH 120
           LSPPLPAISKNLELARAMAA SKSSLFSLSA DV+YEDEWLI VNKPQGVYCESVLAS  
Sbjct: 61  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVVYEDEWLIAVNKPQGVYCESVLASVP 120

Query: 121 RFLGDSAMASLQSGEIKNSFPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 180
            FLGDSAMA  QSGEIK S PELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK
Sbjct: 121 CFLGDSAMAGEQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 180

Query: 181 SYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETHFEVLSV 240
           SYIAFCVGSAPKWKKI +KSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET FEVLSV
Sbjct: 181 SYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSV 240

Query: 241 NGQRSTMEELPDSSKD------QEETIVVQSKSLIDVDAQKHEMLIRARPRSGRTHQIRL 300
           NGQ +T EELP+S KD      +EETIV QSKSLID+DA+K E+LIRARPRSGRTHQIRL
Sbjct: 241 NGQ-NTKEELPESRKDEEEEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRL 300

Query: 301 HCQYLGTPIVGDVKYEGVTEWNGNFFDSHELHAESLYFVHPVTGIPLKLQAPLPLWASQA 360
           H QYLG PIVGDVKYEGVTEWNG  +DSHELHAESLYFVHPVTGIPLKLQAPLP WA+QA
Sbjct: 301 HSQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQA 360

Query: 361 WQPSQQEVNN 364
            QP QQEVN+
Sbjct: 361 LQPQQQEVNS 361

[Close]
BLAST of Sed0005688 vs. NCBI nr
Match: XP_038889468.1 (RNA pseudouridine synthase 1 [Benincasa hispida])

HSP 1 Score: 573.2 bits (1476), Expect = 1.7e-159
Identity = 293/364 (80.49%), Postives = 316/364 (86.81%), Query Frame = 0 

Query: 1   MALFVALQ-LFSFPHKPFTKFPKPFCFRTIEPIGSSMAEAEPSKPVAGNSPATGENYPVP 60
           MAL ++LQ  FS PHK FT+FPKPFC    + I  SMA++  S+PV GN P+T ENYPVP
Sbjct: 1   MALLISLQPTFSTPHKTFTEFPKPFCSGRFKSISKSMADS--SEPVTGNCPSTRENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDEWLIVVNKPQGVYCESVLASAH 120
           LSPPLPAISKNLELARAMAASSKSSLF+LSA+DVIYEDEWLI VNKPQG+YCESVLA+  
Sbjct: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120

Query: 121 RFLGDSAMASLQSGEIKNSFPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 180
           R L DSAMA      IK S PELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSK
Sbjct: 121 RLLSDSAMAG-----IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 180

Query: 181 SYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETHFEVLSV 240
           SYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET+FEVLS+
Sbjct: 181 SYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSI 240

Query: 241 NGQRSTMEELPDSSKDQEETIVVQSKSLIDVDAQKHEMLIRARPRSGRTHQIRLHCQYLG 300
           NGQ + MEE     KD EET+VVQ+KSL+D+D QK E+LIRARPRSGRTHQIRLHCQYLG
Sbjct: 241 NGQ-NAMEEFQKFGKD-EETVVVQTKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLG 300

Query: 301 TPIVGDVKYEGVTEWNGNFFDSHELHAESLYFVHPVTGIPLKLQAPLPLWASQAWQPSQQ 360
            PI GDVKYEGVTEWNG  +D HELHAESLYFVHP+TGIPLKLQAPLP WASQAWQP QQ
Sbjct: 301 IPIRGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWASQAWQPQQQ 355

Query: 361 EVNN 364
           EVN+
Sbjct: 361 EVNS 355

[Close]
BLAST of Sed0005688 vs. NCBI nr
Match: XP_022156345.1 (RNA pseudouridine synthase 1 isoform X2 [Momordica charantia])

HSP 1 Score: 557.8 bits (1436), Expect = 7.2e-155
Identity = 281/325 (86.46%), Postives = 299/325 (92.00%), Query Frame = 0 

Query: 39  AEPSKPVAGNSPATGENYPVPLSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDE 98
           A+PS PV GNSPATG+NYP+PLSPPLPAISKNLELARAMAASSKSSLF+LSA+DVI+EDE
Sbjct: 2   ADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFEDE 61

Query: 99  WLIVVNKPQGVYCESVLASAHRFLGDSAMASLQSGEIKNSFPELHLANRLDRDTSGVMVI 158
           WLI VNKPQGVYCESVLAS  R LGDSAMA  QSG I+ S PELHLANRLDRDTSGVMVI
Sbjct: 62  WLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGIQISLPELHLANRLDRDTSGVMVI 121

Query: 159 TKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVG 218
           TKSHKVASKLVKAFTDH VSKSYIAFCVGSAPKWKKIN+KSGHGRSKFGVWRVYAAADVG
Sbjct: 122 TKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVG 181

Query: 219 RSLPGGSVVRDMETHFEVLSVNGQRSTMEELPDSSKDQEETIVVQSKSLIDVDAQKHEML 278
           RSLPGGSVVRDMET+FEVLSVNGQ STM+ELP+  KD+EETIVVQSKSLID+DA K E+L
Sbjct: 182 RSLPGGSVVRDMETYFEVLSVNGQ-STMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 241

Query: 279 IRARPRSGRTHQIRLHCQYLGTPIVGDVKYEGVTEWNGNFFDSHELHAESLYFVHPVTGI 338
           IRARPRSGRTHQIRLHCQYLG PI GDVKYEGV EWNGN +DSHELHAESL+FVHPVTGI
Sbjct: 242 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 301

Query: 339 PLKLQAPLPLWASQAWQPSQQEVNN 364
           PLKLQAPLP WASQA    QQEV++
Sbjct: 302 PLKLQAPLPSWASQALLSQQQEVSS 325

[Close]
BLAST of Sed0005688 vs. NCBI nr
Match: XP_022156344.1 (RNA pseudouridine synthase 1 isoform X1 [Momordica charantia])

HSP 1 Score: 550.4 bits (1417), Expect = 1.2e-152
Identity = 281/335 (83.88%), Postives = 299/335 (89.25%), Query Frame = 0 

Query: 39  AEPSKPVAGNSPATGENYPVPLSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDE 98
           A+PS PV GNSPATG+NYP+PLSPPLPAISKNLELARAMAASSKSSLF+LSA+DVI+EDE
Sbjct: 2   ADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFEDE 61

Query: 99  WLIVVNKPQGVYCESVLASAHRFLGDSAMASLQSG----------EIKNSFPELHLANRL 158
           WLI VNKPQGVYCESVLAS  R LGDSAMA  QSG           I+ S PELHLANRL
Sbjct: 62  WLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGTTTLCLCLARIQISLPELHLANRL 121

Query: 159 DRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGV 218
           DRDTSGVMVITKSHKVASKLVKAFTDH VSKSYIAFCVGSAPKWKKIN+KSGHGRSKFGV
Sbjct: 122 DRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGV 181

Query: 219 WRVYAAADVGRSLPGGSVVRDMETHFEVLSVNGQRSTMEELPDSSKDQEETIVVQSKSLI 278
           WRVYAAADVGRSLPGGSVVRDMET+FEVLSVNGQ STM+ELP+  KD+EETIVVQSKSLI
Sbjct: 182 WRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQ-STMDELPEVRKDEEETIVVQSKSLI 241

Query: 279 DVDAQKHEMLIRARPRSGRTHQIRLHCQYLGTPIVGDVKYEGVTEWNGNFFDSHELHAES 338
           D+DA K E+LIRARPRSGRTHQIRLHCQYLG PI GDVKYEGV EWNGN +DSHELHAES
Sbjct: 242 DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES 301

Query: 339 LYFVHPVTGIPLKLQAPLPLWASQAWQPSQQEVNN 364
           L+FVHPVTGIPLKLQAPLP WASQA    QQEV++
Sbjct: 302 LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSS 335

[Close]
BLAST of Sed0005688 vs. ExPASy Swiss-Prot
Match: Q7XA65 (RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.3e-95
Identity = 178/299 (59.53%), Postives = 218/299 (72.91%), Query Frame = 0 

Query: 55  NYPVPLSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDEWLIVVNKPQGVYCESV 114
           NYP P+S P P ISK++EL RAM ASSKSSLF+L+  D++YEDE+L+ VNKP+GVYCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 115 LASAHRFLGDSAMASLQSGEIKNSFPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTD 174
           L SA + + DS+              E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+
Sbjct: 99  LRSAPQIVLDSS-------------SEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTE 158

Query: 175 HKVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETHF 234
           HK+ KSYIA C+GS+P W+++ + SGHGRSK G WRVYAA DVGR LPGGS VRDMET F
Sbjct: 159 HKIRKSYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTF 218

Query: 235 EVLSVNGQRSTMEELPDSSKDQEETIVVQSKSLIDVDAQKHE--MLIRARPRSGRTHQIR 294
           EV+SVN  ++   EL    +D    IV + +  +       +  +++RA PRSGRTHQIR
Sbjct: 219 EVVSVNSVKNESCEL----EDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIR 278

Query: 295 LHCQYLGTPIVGDVKYEGVTEWNGNFFDSHELHAESLYFVHPVTGIPLKLQAPLPLWAS 352
           LHCQYLG PI GDVKY GV EWNG  F+ HELHAE L   HPVTG  + ++APLP WA+
Sbjct: 279 LHCQYLGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]
BLAST of Sed0005688 vs. ExPASy Swiss-Prot
Match: Q2QNM3 (RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0560500 PE=2 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 5.1e-95
Identity = 183/310 (59.03%), Postives = 227/310 (73.23%), Query Frame = 0 

Query: 49  SPATGENYPVPLSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDEWLIVVNKPQG 108
           S ATGE YPVP+SPP PA SK++EL RAM AS++S+ +  S++ V++EDEWL VV+KP G
Sbjct: 45  SAATGE-YPVPVSPPYPAASKDVELRRAMTASARSAAY--SSAPVVFEDEWLAVVDKPAG 104

Query: 109 VYCESVLAS---AHRFLGDSAMASLQSGEIKNSFPELHLANRLDRDTSGVMVITKSHKVA 168
           VYC+++L++   +   LGD A           + P LHLANRLDRDTSG+MVITK +KVA
Sbjct: 105 VYCDALLSALPCSAATLGDEA-----------TKPNLHLANRLDRDTSGLMVITKCNKVA 164

Query: 169 SKLVKAFTDHKVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGS 228
            KLVKAFT+HKV K+Y+A C+G  P W+KI I SGHGRSK G WRVYA +DVGRSLPGGS
Sbjct: 165 GKLVKAFTEHKVKKTYLALCIGYPPAWEKIKICSGHGRSKHGAWRVYAMSDVGRSLPGGS 224

Query: 229 VVRDMETHFEVLSVNGQRSTMEELPDSSKDQEETIVVQSKS---LIDVDAQKHEMLIRAR 288
           VVRDM T FEVL +NG +    E  +   D+ E+I VQ K+     D D +   +L+RA 
Sbjct: 225 VVRDMSTRFEVLGING-KGQFREPSNFEVDETESITVQEKAADLTSDGDEKNSIILVRAY 284

Query: 289 PRSGRTHQIRLHCQYLGTPIVGDVKYEGVTEWNGNFFDSHELHAESLYFVHPVTGIPLKL 348
           P+SGRTHQIRLHCQYLG PI GDVKY GV EWNG  +D H LHAESL FVHPVTG+P+  
Sbjct: 285 PQSGRTHQIRLHCQYLGFPIRGDVKYSGVIEWNGVDYDGHALHAESLSFVHPVTGLPVTF 339

Query: 349 QAPLPLWASQ 353
           ++PLP WA++
Sbjct: 345 RSPLPSWANE 339

[Close]
BLAST of Sed0005688 vs. ExPASy Swiss-Prot
Match: Q8PHN2 (Ribosomal large subunit pseudouridine synthase D OS=Xanthomonas axonopodis pv. citri (strain 306) OX=190486 GN=rluD PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 3.0e-18
Identity = 77/266 (28.95%), Postives = 115/266 (43.23%), Query Frame = 0 

Query: 93  VIYEDEWLIVVNKPQGVYCESVLASAHRFLGDSAMASLQSGEIKNSFPELHLANRLDRDT 152
           V+YEDE ++V++KP G+       +  R L     A L       S P   + +RLD+DT
Sbjct: 92  VLYEDEQVLVIDKPAGLVVHPGAGNPDRTL---VNALLFRDPALASVPRAGVVHRLDKDT 151

Query: 153 SGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKINIK-SGHGRSKFGVWRV 212
           SGVMV+ ++ +  + LV+  +  +V + Y+A  VG+       N     H R +  +   
Sbjct: 152 SGVMVVARTVQAQTALVEQLSAREVHRQYLAVVVGALVSGGTANAPIDRHPRDRLKM--- 211

Query: 213 YAAADVGRSLPGGSVVRDMETHFEVLSVNGQRSTMEELPDSSKDQEETIVVQSKSLIDVD 272
            A  D G         RD  TH+ +       + +E                        
Sbjct: 212 -AVRDDG---------RDAVTHYRLRERFRAHTALE------------------------ 271

Query: 273 AQKHEMLIRARPRSGRTHQIRLHCQYLGTPIVGDVKYEGVTEWNGNFFDS--HE------ 332
                     R  +GRTHQIR+H  +L +PIVGD  Y G  +      D   HE      
Sbjct: 272 ---------CRLETGRTHQIRVHMAHLKSPIVGDPLYGGALKLPKGATDELVHELRTFKR 308

Query: 333 --LHAESLYFVHPVTGIPLKLQAPLP 348
             LHAE+L F+HPV+G P++  AP+P
Sbjct: 332 QALHAETLEFLHPVSGEPVRASAPVP 308

[Close]
BLAST of Sed0005688 vs. ExPASy Swiss-Prot
Match: Q87S65 (Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) OX=223926 GN=rluD PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 9.5e-17
Identity = 73/267 (27.34%), Postives = 111/267 (41.57%), Query Frame = 0 

Query: 92  DVIYEDEWLIVVNKPQGVYCESVLASAHRFLGDSAMASLQSGEIKNSFPELHLANRLDRD 151
           D++YED+ +IV+NKP+      V   A    G    A L         P   + +RLD+D
Sbjct: 85  DIVYEDDDIIVINKPRDFV---VHPGAGTPDGTVLNALLHHYPDIAEVPRAGIVHRLDKD 144

Query: 152 TSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRV 211
           T+G+MV+ K+    ++LV+A     +++ Y A  +G      K++   G   +K     +
Sbjct: 145 TTGLMVVAKTVPAQTRLVRALQKRNITREYEAIAIGRMTAGGKVDQPIGRHSTK---RTL 204

Query: 212 YAAADVGRSLPGGSVVRDMETHFEVLSVNGQRSTMEELPDSSKDQEETIVVQSKSLIDVD 271
            A A +G         +   TH+ V                                  +
Sbjct: 205 MAVAPLG---------KPAVTHYRV---------------------------------AE 264

Query: 272 AQKHEMLIRARPRSGRTHQIRLHCQYLGTPIVGDVKYEG-----------VTEWNGNFFD 331
             +    IR R  +GRTHQIR+H  YL  P++GD  Y G           +T+     FD
Sbjct: 265 HFREHTRIRLRLETGRTHQIRVHMSYLQHPLLGDTAYGGRARIPTGASQELTDMIRG-FD 302

Query: 332 SHELHAESLYFVHPVTGIPLKLQAPLP 348
              LHA  L F HP+TG  L+  AP+P
Sbjct: 325 RQALHAVMLRFEHPITGEELEFHAPVP 302

[Close]
BLAST of Sed0005688 vs. ExPASy Swiss-Prot
Match: Q8DEV0 (Ribosomal large subunit pseudouridine synthase D OS=Vibrio vulnificus (strain CMCP6) OX=216895 GN=rluD PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 9.5e-17
Identity = 73/267 (27.34%), Postives = 111/267 (41.57%), Query Frame = 0 

Query: 92  DVIYEDEWLIVVNKPQGVYCESVLASAHRFLGDSAMASLQSGEIKNSFPELHLANRLDRD 151
           D++YED+ ++V+NKP+      V   A    G    A L         P   + +RLD+D
Sbjct: 85  DIVYEDDDILVINKPRDFV---VHPGAGTPDGTVLNALLFHCPEVAEVPRAGIVHRLDKD 144

Query: 152 TSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRV 211
           T+G+MV+ K+    ++LV+A     +++ Y A  +G      K++   G   +K     +
Sbjct: 145 TTGLMVVAKTVPAQTRLVRALQKRNITREYEAIAIGRMTAGGKVDKPIGRHATK---RTL 204

Query: 212 YAAADVGRSLPGGSVVRDMETHFEVLSVNGQRSTMEELPDSSKDQEETIVVQSKSLIDVD 271
            A + +G         +   THF V                                  +
Sbjct: 205 MAVSPMG---------KPAVTHFRV---------------------------------AE 264

Query: 272 AQKHEMLIRARPRSGRTHQIRLHCQYLGTPIVGDVKY-----------EGVTEWNGNFFD 331
             +    IR R  +GRTHQIR+H  YL  P++GD  Y           E VT    N FD
Sbjct: 265 HFREHTRIRLRLETGRTHQIRVHMSYLQHPLLGDAAYGGRARIPAGASEDVTAMIRN-FD 302

Query: 332 SHELHAESLYFVHPVTGIPLKLQAPLP 348
              LHA  L F HP+TG  ++  AP+P
Sbjct: 325 RQALHAVMLRFEHPITGEEMEFHAPVP 302

[Close]
BLAST of Sed0005688 vs. ExPASy TrEMBL
Match: A0A6J1DQ12 (RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 3.5e-155
Identity = 281/325 (86.46%), Postives = 299/325 (92.00%), Query Frame = 0 

Query: 39  AEPSKPVAGNSPATGENYPVPLSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDE 98
           A+PS PV GNSPATG+NYP+PLSPPLPAISKNLELARAMAASSKSSLF+LSA+DVI+EDE
Sbjct: 2   ADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFEDE 61

Query: 99  WLIVVNKPQGVYCESVLASAHRFLGDSAMASLQSGEIKNSFPELHLANRLDRDTSGVMVI 158
           WLI VNKPQGVYCESVLAS  R LGDSAMA  QSG I+ S PELHLANRLDRDTSGVMVI
Sbjct: 62  WLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGIQISLPELHLANRLDRDTSGVMVI 121

Query: 159 TKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVG 218
           TKSHKVASKLVKAFTDH VSKSYIAFCVGSAPKWKKIN+KSGHGRSKFGVWRVYAAADVG
Sbjct: 122 TKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVG 181

Query: 219 RSLPGGSVVRDMETHFEVLSVNGQRSTMEELPDSSKDQEETIVVQSKSLIDVDAQKHEML 278
           RSLPGGSVVRDMET+FEVLSVNGQ STM+ELP+  KD+EETIVVQSKSLID+DA K E+L
Sbjct: 182 RSLPGGSVVRDMETYFEVLSVNGQ-STMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 241

Query: 279 IRARPRSGRTHQIRLHCQYLGTPIVGDVKYEGVTEWNGNFFDSHELHAESLYFVHPVTGI 338
           IRARPRSGRTHQIRLHCQYLG PI GDVKYEGV EWNGN +DSHELHAESL+FVHPVTGI
Sbjct: 242 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 301

Query: 339 PLKLQAPLPLWASQAWQPSQQEVNN 364
           PLKLQAPLP WASQA    QQEV++
Sbjct: 302 PLKLQAPLPSWASQALLSQQQEVSS 325

[Close]
BLAST of Sed0005688 vs. ExPASy TrEMBL
Match: A0A6J1DT56 (RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 5.6e-153
Identity = 281/335 (83.88%), Postives = 299/335 (89.25%), Query Frame = 0 

Query: 39  AEPSKPVAGNSPATGENYPVPLSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDE 98
           A+PS PV GNSPATG+NYP+PLSPPLPAISKNLELARAMAASSKSSLF+LSA+DVI+EDE
Sbjct: 2   ADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFEDE 61

Query: 99  WLIVVNKPQGVYCESVLASAHRFLGDSAMASLQSG----------EIKNSFPELHLANRL 158
           WLI VNKPQGVYCESVLAS  R LGDSAMA  QSG           I+ S PELHLANRL
Sbjct: 62  WLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGTTTLCLCLARIQISLPELHLANRL 121

Query: 159 DRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGV 218
           DRDTSGVMVITKSHKVASKLVKAFTDH VSKSYIAFCVGSAPKWKKIN+KSGHGRSKFGV
Sbjct: 122 DRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGV 181

Query: 219 WRVYAAADVGRSLPGGSVVRDMETHFEVLSVNGQRSTMEELPDSSKDQEETIVVQSKSLI 278
           WRVYAAADVGRSLPGGSVVRDMET+FEVLSVNGQ STM+ELP+  KD+EETIVVQSKSLI
Sbjct: 182 WRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQ-STMDELPEVRKDEEETIVVQSKSLI 241

Query: 279 DVDAQKHEMLIRARPRSGRTHQIRLHCQYLGTPIVGDVKYEGVTEWNGNFFDSHELHAES 338
           D+DA K E+LIRARPRSGRTHQIRLHCQYLG PI GDVKYEGV EWNGN +DSHELHAES
Sbjct: 242 DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES 301

Query: 339 LYFVHPVTGIPLKLQAPLPLWASQAWQPSQQEVNN 364
           L+FVHPVTGIPLKLQAPLP WASQA    QQEV++
Sbjct: 302 LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSS 335

[Close]
BLAST of Sed0005688 vs. ExPASy TrEMBL
Match: A0A0A0LJY6 (PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G033960 PE=4 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 3.6e-152
Identity = 281/366 (76.78%), Postives = 312/366 (85.25%), Query Frame = 0 

Query: 1   MALFVALQ-LFSFPHKPFTKFP--KPFCFRTIEPIGSSMAEAEPSKPVAGNSPATGENYP 60
           MALF++L   FS   K FT+FP  K FCF   + + +SM ++  S+PV GN P+T ENYP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDS--SEPVTGNCPSTAENYP 60

Query: 61  VPLSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDEWLIVVNKPQGVYCESVLAS 120
           VPLSPPLPAISKNLELARAM ASSKSSL++LSA+DVIYEDEWLI VNKPQG+YCE+VLA+
Sbjct: 61  VPLSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAA 120

Query: 121 AHRFLGDSAMASLQSGEIKNSFPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKV 180
             R LGDSA A      IK S PELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH V
Sbjct: 121 VPRLLGDSANAG-----IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTV 180

Query: 181 SKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETHFEVL 240
           SKSYIAFCVG++PKWKKIN+KSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET+FEVL
Sbjct: 181 SKSYIAFCVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVL 240

Query: 241 SVNGQRSTMEELPDSSKDQEETIVVQSKSLIDVDAQKHEMLIRARPRSGRTHQIRLHCQY 300
           SVNG ++TMEEL    + +EETIVV +KSL+D+D+ K E+LIRARPRSGRTHQIRLHCQY
Sbjct: 241 SVNG-KNTMEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQY 300

Query: 301 LGTPIVGDVKYEGVTEWNGNFFDSHELHAESLYFVHPVTGIPLKLQAPLPLWASQAWQPS 360
           LG PI GDVKYEGVTEWN   +DSHELHAESLYFVHPVTGIPLKLQAPLP WASQA QP 
Sbjct: 301 LGIPIRGDVKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQ 358

Query: 361 QQEVNN 364
           Q EVN+
Sbjct: 361 QHEVNS 358

[Close]
BLAST of Sed0005688 vs. ExPASy TrEMBL
Match: A0A6J1FDF4 (RNA pseudouridine synthase 1 OS=Cucurbita moschata OX=3662 GN=LOC111444360 PE=4 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 1.8e-151
Identity = 279/319 (87.46%), Postives = 290/319 (90.91%), Query Frame = 0 

Query: 48  NSPATGENYPVPLSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDEWLIVVNKPQ 107
           + P TGENYPVPLSPPLPAISKNLELARAMAA SKSSLFSLSA DVIYEDEWLI VNKPQ
Sbjct: 5   SKPVTGENYPVPLSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKPQ 64

Query: 108 GVYCESVLASAHRFLGDSAMASLQSGEIKNSFPELHLANRLDRDTSGVMVITKSHKVASK 167
           GVYCESVLAS  RFLGDSAMA  QSGEIK S PELHLANRLDRDTSGVMVITKSHKVASK
Sbjct: 65  GVYCESVLASVPRFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASK 124

Query: 168 LVKAFTDHKVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVV 227
           LVKAFTDHKVSKSYIAFCVGSAPKWKKI +KSGHGRSKFGVWRVYAAADVGRSLPGGSVV
Sbjct: 125 LVKAFTDHKVSKSYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVV 184

Query: 228 RDMETHFEVLSVNGQRSTMEELPDSSKD---QEETIVVQSKSLIDVDAQKHEMLIRARPR 287
           RDMET FEVLSVNGQ +T EE P+S KD   +EETIV QSKSLID+DA+K E+LIRARPR
Sbjct: 185 RDMETSFEVLSVNGQ-NTKEEPPESRKDEEEEEETIVAQSKSLIDIDARKDEILIRARPR 244

Query: 288 SGRTHQIRLHCQYLGTPIVGDVKYEGVTEWNGNFFDSHELHAESLYFVHPVTGIPLKLQA 347
           SGRTHQIRLH QYLG PIVGDVKYEGVTEWNG  +DSHELHAESLYFVHPVTGIPLKLQA
Sbjct: 245 SGRTHQIRLHSQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQA 304

Query: 348 PLPLWASQAWQPSQQEVNN 364
           PLP WA+QA QP QQEVN+
Sbjct: 305 PLPSWATQALQPQQQEVNS 322

[Close]
BLAST of Sed0005688 vs. ExPASy TrEMBL
Match: A0A6J1HPV5 (RNA pseudouridine synthase 1 OS=Cucurbita maxima OX=3661 GN=LOC111465599 PE=4 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 8.9e-151
Identity = 277/319 (86.83%), Postives = 289/319 (90.60%), Query Frame = 0 

Query: 48  NSPATGENYPVPLSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDEWLIVVNKPQ 107
           + P TGENYPVPLSPPLPAISKNLELARAMAA SKSSLFSLSA DVIYEDEWLI VNK Q
Sbjct: 5   SKPVTGENYPVPLSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKTQ 64

Query: 108 GVYCESVLASAHRFLGDSAMASLQSGEIKNSFPELHLANRLDRDTSGVMVITKSHKVASK 167
           GVYCESVL S  RFLGDSAMA  QSGEIK S PELHLANRLDRDTSGVMVITKSHKVASK
Sbjct: 65  GVYCESVLGSVPRFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASK 124

Query: 168 LVKAFTDHKVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVV 227
           LVKAFTDHKVSKSYIAFCVGSAPKWKKI +KSGHGRSKFGVWRVYAAADVGRSLPGGSVV
Sbjct: 125 LVKAFTDHKVSKSYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVV 184

Query: 228 RDMETHFEVLSVNGQRSTMEELPDSSKD---QEETIVVQSKSLIDVDAQKHEMLIRARPR 287
           RDMET FEVLSVNGQ ST EELP+S KD   +EETIV QSKSLID+DA+K E+LIRARPR
Sbjct: 185 RDMETSFEVLSVNGQ-STKEELPESRKDEEEEEETIVAQSKSLIDIDARKDEILIRARPR 244

Query: 288 SGRTHQIRLHCQYLGTPIVGDVKYEGVTEWNGNFFDSHELHAESLYFVHPVTGIPLKLQA 347
           SGRTHQIRLH QYLG PIVGDVKYEGVTEWNG  +DSHELHAESLYFVHP+TGIPLKLQA
Sbjct: 245 SGRTHQIRLHSQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPITGIPLKLQA 304

Query: 348 PLPLWASQAWQPSQQEVNN 364
           PLP WA+QA QP QQE+N+
Sbjct: 305 PLPSWATQALQPQQQEMNS 322

[Close]
BLAST of Sed0005688 vs. TAIR 10
Match: AT1G56345.1 (Pseudouridine synthase family protein )

HSP 1 Score: 351.3 bits (900), Expect = 9.5e-97
Identity = 178/299 (59.53%), Postives = 218/299 (72.91%), Query Frame = 0 

Query: 55  NYPVPLSPPLPAISKNLELARAMAASSKSSLFSLSASDVIYEDEWLIVVNKPQGVYCESV 114
           NYP P+S P P ISK++EL RAM ASSKSSLF+L+  D++YEDE+L+ VNKP+GVYCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 115 LASAHRFLGDSAMASLQSGEIKNSFPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTD 174
           L SA + + DS+              E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+
Sbjct: 99  LRSAPQIVLDSS-------------SEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTE 158

Query: 175 HKVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETHF 234
           HK+ KSYIA C+GS+P W+++ + SGHGRSK G WRVYAA DVGR LPGGS VRDMET F
Sbjct: 159 HKIRKSYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTF 218

Query: 235 EVLSVNGQRSTMEELPDSSKDQEETIVVQSKSLIDVDAQKHE--MLIRARPRSGRTHQIR 294
           EV+SVN  ++   EL    +D    IV + +  +       +  +++RA PRSGRTHQIR
Sbjct: 219 EVVSVNSVKNESCEL----EDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIR 278

Query: 295 LHCQYLGTPIVGDVKYEGVTEWNGNFFDSHELHAESLYFVHPVTGIPLKLQAPLPLWAS 352
           LHCQYLG PI GDVKY GV EWNG  F+ HELHAE L   HPVTG  + ++APLP WA+
Sbjct: 279 LHCQYLGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]
BLAST of Sed0005688 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 70.5 bits (171), Expect = 3.3e-12
Identity = 75/306 (24.51%), Postives = 112/306 (36.60%), Query Frame = 0 

Query: 92  DVIYEDEWLIVVNK------------PQGVYCESVL------------------ASAHRF 151
           D++YED+ ++VVNK            P G     +L                  +    F
Sbjct: 149 DIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETF 208

Query: 152 LGDSAMASLQSGEIKNSFPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSY 211
             D  M +  S    +  P   + +RLD+ T+G++V+ K     + L + F  H + + Y
Sbjct: 209 SDDEEMTTSPSSYAASVRP--GIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVY 268

Query: 212 IAFCVG-SAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETHFEVLSVN 271
           ++   G  +P   +I I  G   S     R+  AA     +PGG              V 
Sbjct: 269 VSLTTGVPSPPQGRIEIPIGRDSSN----RIRMAA-----IPGG--------------VR 328

Query: 272 GQRSTMEELPDSSKDQEETIVVQSKSLIDVDAQKHEMLIRARPRSGRTHQIRLHCQYLGT 331
           G R+                      +I+  A     L+  R  +GRTHQIR H +Y+G 
Sbjct: 329 GGRARH--------------AASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGV 388

Query: 332 PIVGDVKYEGVTEWNGNFF-------DSHE------------LHAESLYFVHPVTGIPLK 348
           P++GD  Y G      +         D  E            LHA  L F HP TG  +K
Sbjct: 389 PLLGDEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVK 415

[Close]
BLAST of Sed0005688 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 60.1 bits (144), Expect = 4.4e-09
Identity = 60/227 (26.43%), Postives = 98/227 (43.17%), Query Frame = 0 

Query: 92  DVIYEDEWLIVVNKPQGVYC--------ESVLASAHRFLG--DSAMASLQSGEIKNSFPE 151
           +V+YED+ LI +NKP G+           +VL       G  DS + S +S       P 
Sbjct: 98  EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRES-------PH 157

Query: 152 LHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKINIKSGH 211
               +RL R TSG+++  K+    +KL   F +           VGS      ++ + G 
Sbjct: 158 PVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEG-------TSLVGSG----NLDQECGT 217

Query: 212 GRSKFGVWRVYAAADVGRSLPGGSVVRDMETHFEVLSVNGQRSTMEELPDSSKDQEETIV 271
           GR           + + R+L  G V  D     + + V       + L  +S + +    
Sbjct: 218 GRK---------LSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAF- 277

Query: 272 VQSKSLIDVDAQKHEMLIRARPRSGRTHQIRLHCQYLGTPIVGDVKY 309
                +++ D +K+  L++   +SGR HQIR+H  Y+G P+VGD  Y
Sbjct: 278 -SKVFVLERDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY 295

[Close]
BLAST of Sed0005688 vs. TAIR 10
Match: AT1G78910.1 (Pseudouridine synthase family protein )

HSP 1 Score: 57.4 bits (137), Expect = 2.8e-08
Identity = 64/254 (25.20%), Postives = 108/254 (42.52%), Query Frame = 0 

Query: 93  VIYEDEWLIVVNKPQGVYCESVLASAHRFLGDSAMASLQSGEIKNSFPELHLANRLDRDT 152
           V ++D  +IV+NKP  +  +  +   H  +   A A+L  G  +       L +RLDR+T
Sbjct: 181 VRFKDSAIIVLNKPPKLPVKGNV-PIHNSMDALAAAALSFGNDEGP----RLVHRLDRET 240

Query: 153 SGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVY 212
           SG++V+ ++ +    L   F+D+K   S       S   W K         +   +++ Y
Sbjct: 241 SGLLVMGRTKESIDYLHSVFSDYKGRNS-------SCKAWNK---------ACEAMYQQY 300

Query: 213 AAADVGRSLPGGSVVRDMETHFEVLSVNGQRSTMEELPDSSKDQEETIVVQSKSLIDVDA 272
            A  +G       ++    +  +VL  +G+   +     S  +  +  + + K L     
Sbjct: 301 WALVIGSPKEKEGLISAPLS--KVLLDDGKTDRVVLAQGSGFEASQDAITEYKVL--GPK 360

Query: 273 QKHEMLIRARPRSGRTHQIRLHC-QYLGTPIVGDVKYEGVTEWNGNFFDSHELHAESLYF 332
                 +  RP + R HQ+R+HC + LGTPIVGD KY         +F            
Sbjct: 361 INGCSWVELRPITSRKHQLRVHCAEALGTPIVGDYKY--------GWFVHKRWKQMPQVD 401

Query: 333 VHPVTGIPLKLQAP 346
           + P TG P KL+ P
Sbjct: 421 IEPTTGKPYKLRRP 401

[Close]
BLAST of Sed0005688 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.4e-07
Identity = 58/229 (25.33%), Postives = 97/229 (42.36%), Query Frame = 0 

Query: 92  DVIYEDEWLIVVNKPQGVYC--------ESVLASAHRFLG--DSAMASLQSGEIKNSFPE 151
           +V+YED+ LI +NKP G+           +VL       G  DS + S +S       P 
Sbjct: 128 EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRES-------PH 187

Query: 152 LHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKINIKSGH 211
               +RL R TSG+++  K+    +KL   F +           VGS      ++ + G 
Sbjct: 188 PVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEG-------TSLVGSG----NLDQECGT 247

Query: 212 GRSKFGVWRVYAAADVGRSLPGGSVVRDMETHFEVLSVNGQRSTMEELPDSSKDQEETIV 271
           GR           + + R+L  G V  D     + + V       + L  +S + +    
Sbjct: 248 GRK---------LSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAF- 307

Query: 272 VQSKSLIDVDAQKHEMLIRARPRSGRTHQIRLHCQYLGTPIVGDVKYEG 311
                +++ D +K+  L++   +SGR HQIR+H  Y+G P+V    + G
Sbjct: 308 -SKVFVLERDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVEMTMFTG 327

The following BLAST results are available for this feature:
BLAST of Sed0005688 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7021056.17.9e-16282.88RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
KAG6586224.15.7e-16081.89RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038889468.11.7e-15980.49RNA pseudouridine synthase 1 [Benincasa hispida][more]
XP_022156345.17.2e-15586.46RNA pseudouridine synthase 1 isoform X2 [Momordica charantia][more]
XP_022156344.11.2e-15283.88RNA pseudouridine synthase 1 isoform X1 [Momordica charantia][more]
BLAST of Sed0005688 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q7XA651.3e-9559.53RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 S... [more]
Q2QNM35.1e-9559.03RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g05... [more]
Q8PHN23.0e-1828.95Ribosomal large subunit pseudouridine synthase D OS=Xanthomonas axonopodis pv. c... [more]
Q87S659.5e-1727.34Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus sero... [more]
Q8DEV09.5e-1727.34Ribosomal large subunit pseudouridine synthase D OS=Vibrio vulnificus (strain CM... [more]
BLAST of Sed0005688 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DQ123.5e-15586.46RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1DT565.6e-15383.88RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A0A0LJY63.6e-15276.78PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G03... [more]
A0A6J1FDF41.8e-15187.46RNA pseudouridine synthase 1 OS=Cucurbita moschata OX=3662 GN=LOC111444360 PE=4 ... [more]
A0A6J1HPV58.9e-15186.83RNA pseudouridine synthase 1 OS=Cucurbita maxima OX=3661 GN=LOC111465599 PE=4 SV... [more]
BLAST of Sed0005688 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G56345.19.5e-9759.53Pseudouridine synthase family protein [more]
AT1G76050.23.3e-1224.51Pseudouridine synthase family protein [more]
AT3G52260.24.4e-0926.43Pseudouridine synthase family protein [more]
AT1G78910.12.8e-0825.20Pseudouridine synthase family protein [more]
AT3G52260.31.4e-0725.33Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 87..348
e-value: 9.0E-49
score: 168.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 37..57
NoneNo IPR availablePANTHERPTHR21600:SF47RNA PSEUDOURIDINE SYNTHASE 1coord: 57..357
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 57..357
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 100..296
e-value: 1.0E-24
score: 87.5
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 100..331
e-value: 2.10892E-44
score: 149.795
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 145..159
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 91..356

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0005688.1Sed0005688.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding