Sed0005304 (gene) Chayote v1

Overview
NameSed0005304
Typegene
OrganismSechium edule (Chayote v1)
Descriptionbifunctional TH2 protein, mitochondrial-like
LocationLG08: 25851181 .. 25854719 (-)
RNA-Seq ExpressionSed0005304
SyntenySed0005304
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAAAGAAAAAGGGGAATTGGTCTATCAAAACTTTCCCTCCCTCGTTCCCTTTTCACACATCCATTCCCAAATACAGCCGCCGTCTCATCCATCCCTTTCCGATGCTCTCTCCGATCGTCTCCAAAATCCCACTCAACACCGCTTTTCTCCTCTCCAATTCCCGATTCCCGATCGCTGCCGCTCTCCGATCCCTCTCTTCCGGCTCCGCTCACCGATCTCCGCCTCCTCATTCGACCATGATTCCGCCTCTCGACTCCCAACTCCCCCTCCCTCAGAGACTGTGGAATAACTGCCGCAGGGAGTCCATATTATCGATGTACACCCCTTTCTGCCTTGCTCTCGCTTCCGGAACCCTCCATATCGACGCATTTCGCCGCTATATCGACCAGGATGTTCATTTCCTCAACGCGTTTGGCCGAGCGTCAGTTCCTTTTCTTTTTTCTTTTTTATGTTGGTTATTGTGTTGTTGGATGGTGCCGATGGTTTGATTTCAATTGTGATCTCTGTTTCTTAAATTTAGGTCTGTTTTTCTGCTGCCAAATATTTTGCAATTAATTTATAGGGCCTGGAGTTTTCTTTTTGATCGGCTTCAGAATCTAGACCCCTTTAATTTAATTCAAAAAATTTCCGGTGCCTGGCCTAGGGACGGACGCGGTTACCTTGATTCAAAAAAAAAAAATTTAATTCAAAAAATTAGTTGGGAAGACTTGTATGGGCGAAATTTTCATGATGACCACAGCAAGGAAGAGAAATAGTTTCAGCAATATATTCCACTGTGCTTTAATTTTTGTTTCAAAGATTTCTTGAGTTTGAATACTTTTGATGTGTTGTTGCTGCAAACGTTGTTCTATTGAGAGAAGTAGAATTAGTTTCTTTTTGGTGAAATTAAGAAGTATTGGATCATGAAACTGAGAATTGGGGTCGTGTTCTAGTTTTAAAGATGTGATCCGTCTGTGTTTTCTAAGATTTTTCTTAGAATCCAATGGAAGAATGTAAATGCCCTTATTTTATTCTAATCTAGGTATGAATTAGCTGCTGAATGTGCTGATGATGATGAGGCAAGACATTCAATCAATGAGCTGAGAAATGCAGTGACTGAAGAACTGAAAATGCATGCTTCATTTGTTAAGGTAGAAGGGCATCATTATTTTTCATTGTAAACTTCCACGATCGCCTTCGTTCTCATCGATTTCCTCACATTTATCGCTTTTATTTGAAGAACAAAGCCTTTTATAACCTAGTATTTTAATGGAATTCGGCCCATTTTGATCTGTTTTCATCTTTTTATTGTATTGGTAAGTTATATGACTCTATTGGCTTGGTGAATGAACTTTAAGGAATGGGGTGCTGAGGAAGGAATAGAATCTCATGTCAGCCCTGCTACTCTCAAATACACAGAGTTTTTGTTGGCAACGGCATCTGGCAAGATTGAAGGGTCTGAAGGTCTTGGTAATCTTGCAACCCCTTTCGAAAGAACAAAGCTTGCTGCTTATACTCTTGGTGCCATGACGCCTTGCATGAGACTGTATGCCTATTTAGCTACGGAGTTTAAGGGAGCTCTTGGTGCTGACTATGGTGATCATCCTTACAAGACTTGGATTGAAAACTACGCATCTAAAGGCTTTGAGGTTTTTTATTTTGAGTTGTGAAGTTTCTCTCTTTGTCTTGTATTGAAGATGGATTACTTAAATGGCTTGCCATATTTATTTTCAATTCAGGAAGCAGCTGAGAGAACCGAAGACGTGCTTGAGAAACTGTCTGCTACTTTGACCGGTGAAGAGCTTGACACCATCGAGAAGCTTTATCACCAAGCTATGAAACTCGAGCTAGAATTTTTCTACTCTCAACCTGTTCCCCATAAGACTGTAGTTCCTCTAATTAAAGATCATAATCCTGCAGAGGATCGGTTGGTATTCTTTTCTGATTTTGATTTGACGTGTACTATGGTCGATTCATCTGCCATTCTGGCAGAACTTGCAATAATAAGAGCTCCAAAACTCGATCAGAATCAACCTTCTGAAGACCAAGTTACCCGGATGTCATCAGCCGATCTAAAAAAATCATGGGGTGTTATTTCTAGGCAGTACACAGAAGAGTATGAAGACTGCATCAAACAAATCACGCCCCCTCAAACAGGTAGTTAGTAACATGGGTTTTCTTTAATCCAATGATTTTTGTGAATATAATTGCTAGTCAGTATTTTATTATTAATATCTTGAATTTTTGGGCCTTGTAGAGGAATTTAAGTATGAAACTCTGTGTACCGCTCTTGAGCAGCTTTCTGATTTTGAGAAAAAGGCAAATAGCAGAGTTATAGAGTCTGGAGTGCTTAAGAACTTAAATTTCGAAGATATAAGACGAGCTGGTGAACATCTTCTTATCCAAGATGGTTGTTTCAATTTCTTTGGAACGGCTAATAAAAATGAAAATTTGAATGTTGCTATCAACATAGTGTCTTATTGTTGGTGCGGGGATTTCATCAGGTCGTCTCTTAAGTCAGGTACATTTCTTTACTCTCTTTAAATAGATGTGGAGATGACGATTGCTGGGTTTTTTAGATGAATTAAAAACTGTTATATTTGTTAAGCCTGTCATATTCAATTCCTTCCAAGCTTTCTAATTTCTTTTTGCAATTTTGGGAGAGAAATAATAATAATTGTTGAACTAGCATGTTCGGTTATTATACAAAGAATCATGATTTTGTTTTATATTTTTGTCAGGTGGTTTACTTAATCAAGTGACTTTACATGCCAATGAATTTATCTTTGAAGAATCGGTTTCTACGGGTGATTTAGTTAGGAAGGTAGAATCCCCGATTGAAAAAGTCGATGTTTTTCGAAAAACCTTGGAGAGCTACGACAATGATAGAAAGAACTTCACAATATATGTCGGAGACTCGATTGGTGACTTGCTTTGCCTACTGGAAGCAGATGTAGGAATTGTTATTGGGTCAAGTTCCAGTCTAAAAAGATTGGCTACGCAGTTCGGAGTTTCTTTCGTTCCTCTGTACCAACAAGTAGTAAGAAAACAGAAAGCTCTTACCGAAGAGACAAGATGCAGTTGGAAAGGATTACCTGGTGTCCTTTACACTGTCAATACTTGGGCTGAAATTCATGCTTTTGTTCTTGGATGCTAGAGCCAAGCTTTTTTTTTTTGTGGTTCTCATTCATCTCAACTCGTTTGGTTTTCGAGACTGTTTTTGTATTGTCGATAGTAAAGAAGATACAAGTTGATGGTGAATTTTATAGGGCCAACAACATCGTTCTGCTGTCGTCCTTCCTTCCGTACTTGGGAAGCTTTCCTGTATTACATGGTAAGTTTAGAATTATCTTGTAAAATTTTGAATCAGGTTATTGTGAGGAAGGATTGAGAGCAATTATTTGGAAAGATAAATTGTCCATATAAAACATTTCAATCTCTGTATCCATGGATTGATAGTGATAGGTGCTGTTCTATATTTTGAGATGAATGGCAGTTTACACAGGGTGTTGTAAATTTTACTACCTAATATTATGAATGAAATGCACAAAACTTTTG

mRNA sequence

GAAAAAGAAAAAGGGGAATTGGTCTATCAAAACTTTCCCTCCCTCGTTCCCTTTTCACACATCCATTCCCAAATACAGCCGCCGTCTCATCCATCCCTTTCCGATGCTCTCTCCGATCGTCTCCAAAATCCCACTCAACACCGCTTTTCTCCTCTCCAATTCCCGATTCCCGATCGCTGCCGCTCTCCGATCCCTCTCTTCCGGCTCCGCTCACCGATCTCCGCCTCCTCATTCGACCATGATTCCGCCTCTCGACTCCCAACTCCCCCTCCCTCAGAGACTGTGGAATAACTGCCGCAGGGAGTCCATATTATCGATGTACACCCCTTTCTGCCTTGCTCTCGCTTCCGGAACCCTCCATATCGACGCATTTCGCCGCTATATCGACCAGGATGTTCATTTCCTCAACGCGTTTGGCCGAGCGTATGAATTAGCTGCTGAATGTGCTGATGATGATGAGGCAAGACATTCAATCAATGAGCTGAGAAATGCAGTGACTGAAGAACTGAAAATGCATGCTTCATTTGTTAAGGAATGGGGTGCTGAGGAAGGAATAGAATCTCATGTCAGCCCTGCTACTCTCAAATACACAGAGTTTTTGTTGGCAACGGCATCTGGCAAGATTGAAGGGTCTGAAGGTCTTGGTAATCTTGCAACCCCTTTCGAAAGAACAAAGCTTGCTGCTTATACTCTTGGTGCCATGACGCCTTGCATGAGACTGTATGCCTATTTAGCTACGGAGTTTAAGGGAGCTCTTGGTGCTGACTATGGTGATCATCCTTACAAGACTTGGATTGAAAACTACGCATCTAAAGGCTTTGAGGAAGCAGCTGAGAGAACCGAAGACGTGCTTGAGAAACTGTCTGCTACTTTGACCGGTGAAGAGCTTGACACCATCGAGAAGCTTTATCACCAAGCTATGAAACTCGAGCTAGAATTTTTCTACTCTCAACCTGTTCCCCATAAGACTGTAGTTCCTCTAATTAAAGATCATAATCCTGCAGAGGATCGGTTGGTATTCTTTTCTGATTTTGATTTGACGTGTACTATGGTCGATTCATCTGCCATTCTGGCAGAACTTGCAATAATAAGAGCTCCAAAACTCGATCAGAATCAACCTTCTGAAGACCAAGTTACCCGGATGTCATCAGCCGATCTAAAAAAATCATGGGGTGTTATTTCTAGGCAGTACACAGAAGAGTATGAAGACTGCATCAAACAAATCACGCCCCCTCAAACAGAGGAATTTAAGTATGAAACTCTGTGTACCGCTCTTGAGCAGCTTTCTGATTTTGAGAAAAAGGCAAATAGCAGAGTTATAGAGTCTGGAGTGCTTAAGAACTTAAATTTCGAAGATATAAGACGAGCTGGTGAACATCTTCTTATCCAAGATGGTTGTTTCAATTTCTTTGGAACGGCTAATAAAAATGAAAATTTGAATGTTGCTATCAACATAGTGTCTTATTGTTGGTGCGGGGATTTCATCAGGTCGTCTCTTAAGTCAGGTGGTTTACTTAATCAAGTGACTTTACATGCCAATGAATTTATCTTTGAAGAATCGGTTTCTACGGGTGATTTAGTTAGGAAGGTAGAATCCCCGATTGAAAAAGTCGATGTTTTTCGAAAAACCTTGGAGAGCTACGACAATGATAGAAAGAACTTCACAATATATGTCGGAGACTCGATTGGTGACTTGCTTTGCCTACTGGAAGCAGATGTAGGAATTGTTATTGGGTCAAGTTCCAGTCTAAAAAGATTGGCTACGCAGTTCGGAGTTTCTTTCGTTCCTCTGTACCAACAAGTAGTAAGAAAACAGAAAGCTCTTACCGAAGAGACAAGATGCAGTTGGAAAGGATTACCTGGTGTCCTTTACACTGTCAATACTTGGGCTGAAATTCATGCTTTTGTTCTTGGATGCTAGAGCCAAGCTTTTTTTTTTTGTGGTTCTCATTCATCTCAACTCGTTTGGTTTTCGAGACTGTTTTTGTATTGTCGATAGTAAAGAAGATACAAGTTGATGGTGAATTTTATAGGGCCAACAACATCGTTCTGCTGTCGTCCTTCCTTCCGTACTTGGGAAGCTTTCCTGTATTACATGGTAAGTTTAGAATTATCTTGTAAAATTTTGAATCAGGTTATTGTGAGGAAGGATTGAGAGCAATTATTTGGAAAGATAAATTGTCCATATAAAACATTTCAATCTCTGTATCCATGGATTGATAGTGATAGGTGCTGTTCTATATTTTGAGATGAATGGCAGTTTACACAGGGTGTTGTAAATTTTACTACCTAATATTATGAATGAAATGCACAAAACTTTTG

Coding sequence (CDS)

ATGCTCTCTCCGATCGTCTCCAAAATCCCACTCAACACCGCTTTTCTCCTCTCCAATTCCCGATTCCCGATCGCTGCCGCTCTCCGATCCCTCTCTTCCGGCTCCGCTCACCGATCTCCGCCTCCTCATTCGACCATGATTCCGCCTCTCGACTCCCAACTCCCCCTCCCTCAGAGACTGTGGAATAACTGCCGCAGGGAGTCCATATTATCGATGTACACCCCTTTCTGCCTTGCTCTCGCTTCCGGAACCCTCCATATCGACGCATTTCGCCGCTATATCGACCAGGATGTTCATTTCCTCAACGCGTTTGGCCGAGCGTATGAATTAGCTGCTGAATGTGCTGATGATGATGAGGCAAGACATTCAATCAATGAGCTGAGAAATGCAGTGACTGAAGAACTGAAAATGCATGCTTCATTTGTTAAGGAATGGGGTGCTGAGGAAGGAATAGAATCTCATGTCAGCCCTGCTACTCTCAAATACACAGAGTTTTTGTTGGCAACGGCATCTGGCAAGATTGAAGGGTCTGAAGGTCTTGGTAATCTTGCAACCCCTTTCGAAAGAACAAAGCTTGCTGCTTATACTCTTGGTGCCATGACGCCTTGCATGAGACTGTATGCCTATTTAGCTACGGAGTTTAAGGGAGCTCTTGGTGCTGACTATGGTGATCATCCTTACAAGACTTGGATTGAAAACTACGCATCTAAAGGCTTTGAGGAAGCAGCTGAGAGAACCGAAGACGTGCTTGAGAAACTGTCTGCTACTTTGACCGGTGAAGAGCTTGACACCATCGAGAAGCTTTATCACCAAGCTATGAAACTCGAGCTAGAATTTTTCTACTCTCAACCTGTTCCCCATAAGACTGTAGTTCCTCTAATTAAAGATCATAATCCTGCAGAGGATCGGTTGGTATTCTTTTCTGATTTTGATTTGACGTGTACTATGGTCGATTCATCTGCCATTCTGGCAGAACTTGCAATAATAAGAGCTCCAAAACTCGATCAGAATCAACCTTCTGAAGACCAAGTTACCCGGATGTCATCAGCCGATCTAAAAAAATCATGGGGTGTTATTTCTAGGCAGTACACAGAAGAGTATGAAGACTGCATCAAACAAATCACGCCCCCTCAAACAGAGGAATTTAAGTATGAAACTCTGTGTACCGCTCTTGAGCAGCTTTCTGATTTTGAGAAAAAGGCAAATAGCAGAGTTATAGAGTCTGGAGTGCTTAAGAACTTAAATTTCGAAGATATAAGACGAGCTGGTGAACATCTTCTTATCCAAGATGGTTGTTTCAATTTCTTTGGAACGGCTAATAAAAATGAAAATTTGAATGTTGCTATCAACATAGTGTCTTATTGTTGGTGCGGGGATTTCATCAGGTCGTCTCTTAAGTCAGGTGGTTTACTTAATCAAGTGACTTTACATGCCAATGAATTTATCTTTGAAGAATCGGTTTCTACGGGTGATTTAGTTAGGAAGGTAGAATCCCCGATTGAAAAAGTCGATGTTTTTCGAAAAACCTTGGAGAGCTACGACAATGATAGAAAGAACTTCACAATATATGTCGGAGACTCGATTGGTGACTTGCTTTGCCTACTGGAAGCAGATGTAGGAATTGTTATTGGGTCAAGTTCCAGTCTAAAAAGATTGGCTACGCAGTTCGGAGTTTCTTTCGTTCCTCTGTACCAACAAGTAGTAAGAAAACAGAAAGCTCTTACCGAAGAGACAAGATGCAGTTGGAAAGGATTACCTGGTGTCCTTTACACTGTCAATACTTGGGCTGAAATTCATGCTTTTGTTCTTGGATGCTAG

Protein sequence

MLSPIVSKIPLNTAFLLSNSRFPIAAALRSLSSGSAHRSPPPHSTMIPPLDSQLPLPQRLWNNCRRESILSMYTPFCLALASGTLHIDAFRRYIDQDVHFLNAFGRAYELAAECADDDEARHSINELRNAVTEELKMHASFVKEWGAEEGIESHVSPATLKYTEFLLATASGKIEGSEGLGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYGDHPYKTWIENYASKGFEEAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFFYSQPVPHKTVVPLIKDHNPAEDRLVFFSDFDLTCTMVDSSAILAELAIIRAPKLDQNQPSEDQVTRMSSADLKKSWGVISRQYTEEYEDCIKQITPPQTEEFKYETLCTALEQLSDFEKKANSRVIESGVLKNLNFEDIRRAGEHLLIQDGCFNFFGTANKNENLNVAINIVSYCWCGDFIRSSLKSGGLLNQVTLHANEFIFEESVSTGDLVRKVESPIEKVDVFRKTLESYDNDRKNFTIYVGDSIGDLLCLLEADVGIVIGSSSSLKRLATQFGVSFVPLYQQVVRKQKALTEETRCSWKGLPGVLYTVNTWAEIHAFVLGC
Homology
BLAST of Sed0005304 vs. NCBI nr
Match: XP_038880589.1 (bifunctional TH2 protein, mitochondrial [Benincasa hispida])

HSP 1 Score: 958.7 bits (2477), Expect = 2.3e-275
Identity = 481/612 (78.59%), Postives = 532/612 (86.93%), Query Frame = 0

Query: 1   MLSPIVSKIPLNTAFLLSNSRFPI----AAALRSLSSGSAHRSP---PPHSTMIPPLDSQ 60
           M S  +SK P        NSRFPI    +AA RS S  SA  SP   PP   MI  +DS 
Sbjct: 1   MRSLFISKFP-------PNSRFPIPIAVSAAFRSRSPPSATPSPPEMPPRLAMISHVDSD 60

Query: 61  LPLPQRLWNNCRRESILSMYTPFCLALASGTLHIDAFRRYIDQDVHFLNAFGRAYELAAE 120
            PL +RLWN CRRESILSMYTPFC+ LASG L+ID FR YIDQDVHFL AFGRAYELAAE
Sbjct: 61  GPLARRLWNKCRRESILSMYTPFCVCLASGALNIDTFRHYIDQDVHFLKAFGRAYELAAE 120

Query: 121 CADDDEARHSINELRNAVTEELKMHASFVKEWGAEEGIESHVSPATLKYTEFLLATASGK 180
           CADDD+A+HSINELR AV+ ELKMHASFVKEW AE+G ES V+PAT+KYT+FLLATASGK
Sbjct: 121 CADDDDAKHSINELRKAVSVELKMHASFVKEWAAEDGKESPVNPATVKYTDFLLATASGK 180

Query: 181 IEGSEGLGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYGDHPYKTWIEN 240
           IEG++ LGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGAL AD+GDHPYKTWIEN
Sbjct: 181 IEGAKALGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALRADHGDHPYKTWIEN 240

Query: 241 YASKGFEEAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFFYSQPVPHKTVVPL 300
           Y+SKGFE AAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLE EFF SQP+  +TVVPL
Sbjct: 241 YSSKGFEAAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLEQEFFCSQPISQRTVVPL 300

Query: 301 IKDHNPAEDRLVFFSDFDLTCTMVDSSAILAELAIIRAPKLDQNQPSEDQVTRMSSADLK 360
           IKDHNPAEDRLV FSDFDLTCT+VDSSAILAE+AI+RAPK DQ QP +  +TRMSSADL+
Sbjct: 301 IKDHNPAEDRLVLFSDFDLTCTVVDSSAILAEIAIVRAPKPDQIQPEDQVITRMSSADLR 360

Query: 361 KSWGVISRQYTEEYEDCIKQITPPQTEEFKYETLCTALEQLSDFEKKANSRVIESGVLKN 420
            +WGVISRQYTEEYEDCI +I PP+TEEFK+E LCTALEQLSDFEK+AN+RVIESGVLK 
Sbjct: 361 NTWGVISRQYTEEYEDCIDKILPPKTEEFKFEDLCTALEQLSDFEKRANNRVIESGVLKG 420

Query: 421 LNFEDIRRAGEHLLIQDGCFNFFGTANKNENLNVAINIVSYCWCGDFIRSSLKSGGLLNQ 480
           LNFEDIRRAGEHL+IQDGCFNFFGTA K+ENLNV ++I+SYCWC D IRSS  SGGLL Q
Sbjct: 421 LNFEDIRRAGEHLIIQDGCFNFFGTACKSENLNVGLHILSYCWCADLIRSSFNSGGLLTQ 480

Query: 481 VTLHANEFIFEESVSTGDLVRKVESPIEKVDVFRKTLESYDNDRKNFTIYVGDSIGDLLC 540
            T+HANEF FEE+VSTGDLVRK+ESP++K++ FRK LE+Y ND+KN T+YVGDSIGDLLC
Sbjct: 481 ATIHANEFAFEEAVSTGDLVRKIESPLDKLNAFRKILENYGNDKKNLTVYVGDSIGDLLC 540

Query: 541 LLEADVGIVIGSSSSLKRLATQFGVSFVPLYQQVVRKQKALTEETRCSWKGLPGVLYTVN 600
           LLEAD+GIVIGSS+SL+RLAT+FGVSFVPLY  VVRKQK LTEETR SWKGL GVLYTVN
Sbjct: 541 LLEADIGIVIGSSASLRRLATRFGVSFVPLYPSVVRKQKELTEETRRSWKGLSGVLYTVN 600

Query: 601 TWAEIHAFVLGC 606
           +WAEIHAFVLGC
Sbjct: 601 SWAEIHAFVLGC 605

BLAST of Sed0005304 vs. NCBI nr
Match: KAA0038327.1 (putative aminopyrimidine aminohydrolase [Cucumis melo var. makuwa] >TYJ97070.1 putative aminopyrimidine aminohydrolase [Cucumis melo var. makuwa])

HSP 1 Score: 956.4 bits (2471), Expect = 1.1e-274
Identity = 476/616 (77.27%), Postives = 531/616 (86.20%), Query Frame = 0

Query: 1   MLSPIVSKIPLNTAFLLSNSRFP-IAAALRSLSSGSAHRSP----------PPHSTMIPP 60
           M +  + K PL++ F+  NS FP +  A RSLS  S HRSP          PP   MI  
Sbjct: 1   MRTLFIPKFPLSSLFVFPNSPFPFLIDAFRSLSFHSLHRSPSSAASSSPTMPPRLAMISH 60

Query: 61  LDSQLPLPQRLWNNCRRESILSMYTPFCLALASGTLHIDAFRRYIDQDVHFLNAFGRAYE 120
           +DS  PL +RLWN CRRESILSMYTPFC+ LA GTL+ID FR YIDQDVHFL AF RAYE
Sbjct: 61  VDSDGPLARRLWNKCRRESILSMYTPFCVCLACGTLNIDTFRHYIDQDVHFLKAFARAYE 120

Query: 121 LAAECADDDEARHSINELRNAVTEELKMHASFVKEWGAEEGIESHVSPATLKYTEFLLAT 180
           LAAECADDD+A+HSINELR AV+ ELKMHASFVKEW A +G ES V+PAT+KYT+FLLAT
Sbjct: 121 LAAECADDDDAKHSINELRKAVSVELKMHASFVKEWAAADGKESPVNPATIKYTDFLLAT 180

Query: 181 ASGKIEGSEGLGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYGDHPYKT 240
           ASGKIEG+EGLGNLATPFERTKLAAY LGAMTPCMRLYAYLATEFKG LGA +GDHPYKT
Sbjct: 181 ASGKIEGAEGLGNLATPFERTKLAAYALGAMTPCMRLYAYLATEFKGVLGALHGDHPYKT 240

Query: 241 WIENYASKGFEEAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFFYSQPVPHKT 300
           WIENYASKGFEEAAE+TEDVLEKLSATLTGEELDTIEKLYHQAMKLE EFF SQPV  KT
Sbjct: 241 WIENYASKGFEEAAEKTEDVLEKLSATLTGEELDTIEKLYHQAMKLEQEFFCSQPVSQKT 300

Query: 301 VVPLIKDHNPAEDRLVFFSDFDLTCTMVDSSAILAELAIIRAPKLDQNQPSEDQVTRMSS 360
           V+PLIKDHNPAEDRLV FSDFDLTCT+VDSSAILAE+AI+RAPK DQ QP +   TRMSS
Sbjct: 301 VLPLIKDHNPAEDRLVLFSDFDLTCTVVDSSAILAEIAIVRAPKPDQIQPEDQPFTRMSS 360

Query: 361 ADLKKSWGVISRQYTEEYEDCIKQITPPQTEEFKYETLCTALEQLSDFEKKANSRVIESG 420
           ADL+ +WGVISRQYTEEYE+CI ++ PP+T EFK+E LCTALEQLSDFEK+AN+RV+ESG
Sbjct: 361 ADLRNTWGVISRQYTEEYEECIDKVMPPKTVEFKFEDLCTALEQLSDFEKRANNRVVESG 420

Query: 421 VLKNLNFEDIRRAGEHLLIQDGCFNFFGTANKNENLNVAINIVSYCWCGDFIRSSLKSGG 480
           VLK LNFEDIRRAGEHL+IQDGCFNFFGTA K+ENLNV ++I+SYCWC D IRSS  SGG
Sbjct: 421 VLKGLNFEDIRRAGEHLIIQDGCFNFFGTACKSENLNVGVHILSYCWCADLIRSSFNSGG 480

Query: 481 LLNQVTLHANEFIFEESVSTGDLVRKVESPIEKVDVFRKTLESYDNDRKNFTIYVGDSIG 540
           LL QVT+HANEF FEE+VSTGDLVR+VESP++KV+ FRK LE+Y NDR N T+Y+GDSIG
Sbjct: 481 LLTQVTIHANEFAFEEAVSTGDLVRRVESPLDKVNAFRKILENYGNDRNNLTVYIGDSIG 540

Query: 541 DLLCLLEADVGIVIGSSSSLKRLATQFGVSFVPLYQQVVRKQKALTEETRCSWKGLPGVL 600
           DLLCLLEAD+GIVIGSS+SL+RLATQFGVSFVPLY  VVRKQK LTE++R SWKGL GVL
Sbjct: 541 DLLCLLEADIGIVIGSSASLRRLATQFGVSFVPLYPSVVRKQKDLTEDSRRSWKGLSGVL 600

Query: 601 YTVNTWAEIHAFVLGC 606
           YTVN+WAEIHAFVLGC
Sbjct: 601 YTVNSWAEIHAFVLGC 616

BLAST of Sed0005304 vs. NCBI nr
Match: XP_008443738.1 (PREDICTED: probable aminopyrimidine aminohydrolase, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 954.9 bits (2467), Expect = 3.3e-274
Identity = 475/616 (77.11%), Postives = 531/616 (86.20%), Query Frame = 0

Query: 1   MLSPIVSKIPLNTAFLLSNSRFP-IAAALRSLSSGSAHRSP----------PPHSTMIPP 60
           M +  + K PL++ F+  NS FP +  A RSLS  S HRSP          PP   MI  
Sbjct: 1   MRTLFIPKFPLSSLFVFPNSPFPFLIDAFRSLSFHSLHRSPSSAASSSPTMPPRLAMISH 60

Query: 61  LDSQLPLPQRLWNNCRRESILSMYTPFCLALASGTLHIDAFRRYIDQDVHFLNAFGRAYE 120
           +DS  PL +RLWN CRRESILSMYTPFC+ LA GTL+ID FR YIDQDVHFL AF RAYE
Sbjct: 61  VDSDGPLARRLWNKCRRESILSMYTPFCVCLACGTLNIDTFRHYIDQDVHFLKAFARAYE 120

Query: 121 LAAECADDDEARHSINELRNAVTEELKMHASFVKEWGAEEGIESHVSPATLKYTEFLLAT 180
           LAAECADDD+A+HSINELR AV+ ELKMHASFVKEW A +G ES V+PAT+KYT+FLLAT
Sbjct: 121 LAAECADDDDAKHSINELRKAVSVELKMHASFVKEWAAADGKESPVNPATIKYTDFLLAT 180

Query: 181 ASGKIEGSEGLGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYGDHPYKT 240
           ASGKIEG+EGLGNLATPFERTKLAAY LGAMTPCMRLYAYLATEFKG LGA +GDHPYKT
Sbjct: 181 ASGKIEGAEGLGNLATPFERTKLAAYALGAMTPCMRLYAYLATEFKGVLGALHGDHPYKT 240

Query: 241 WIENYASKGFEEAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFFYSQPVPHKT 300
           WIENYASKGFEEAAE+TEDVLEKLSATLTGEELDTIEKLYHQAMKLE EFF SQPV  KT
Sbjct: 241 WIENYASKGFEEAAEKTEDVLEKLSATLTGEELDTIEKLYHQAMKLEQEFFCSQPVSQKT 300

Query: 301 VVPLIKDHNPAEDRLVFFSDFDLTCTMVDSSAILAELAIIRAPKLDQNQPSEDQVTRMSS 360
           V+PLIKDHNPAEDRLV FSDFDLTCT+VDSSAILAE+AI+RAPK DQ QP +   TRMSS
Sbjct: 301 VLPLIKDHNPAEDRLVLFSDFDLTCTVVDSSAILAEIAIVRAPKPDQIQPEDQPFTRMSS 360

Query: 361 ADLKKSWGVISRQYTEEYEDCIKQITPPQTEEFKYETLCTALEQLSDFEKKANSRVIESG 420
           ADL+ +WGVISRQYTEEYE+CI ++ PP+T EFK+E LCTALEQLSDFEK+AN+RV+ESG
Sbjct: 361 ADLRNTWGVISRQYTEEYEECIDKVMPPKTVEFKFEDLCTALEQLSDFEKRANNRVVESG 420

Query: 421 VLKNLNFEDIRRAGEHLLIQDGCFNFFGTANKNENLNVAINIVSYCWCGDFIRSSLKSGG 480
           VLK LNFEDIRRAGEHL+IQDGCFNFFGTA K+ENLNV ++I+SYCWC D IRSS  SGG
Sbjct: 421 VLKGLNFEDIRRAGEHLIIQDGCFNFFGTACKSENLNVGVHILSYCWCADLIRSSFNSGG 480

Query: 481 LLNQVTLHANEFIFEESVSTGDLVRKVESPIEKVDVFRKTLESYDNDRKNFTIYVGDSIG 540
           LL QVT+HANEF FEE+VSTGDLVR+VESP++KV+ FRK LE+Y NDR N T+Y+GDSIG
Sbjct: 481 LLTQVTIHANEFAFEEAVSTGDLVRRVESPLDKVNAFRKILENYGNDRNNLTVYIGDSIG 540

Query: 541 DLLCLLEADVGIVIGSSSSLKRLATQFGVSFVPLYQQVVRKQKALTEETRCSWKGLPGVL 600
           DLLCLLEAD+GIVIGSS+SL+RLAT+FGVSFVPLY  VVRKQK LTE++R SWKGL GVL
Sbjct: 541 DLLCLLEADIGIVIGSSASLRRLATRFGVSFVPLYPSVVRKQKDLTEDSRRSWKGLSGVL 600

Query: 601 YTVNTWAEIHAFVLGC 606
           YTVN+WAEIHAFVLGC
Sbjct: 601 YTVNSWAEIHAFVLGC 616

BLAST of Sed0005304 vs. NCBI nr
Match: XP_023515856.1 (bifunctional TH2 protein, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 953.0 bits (2462), Expect = 1.3e-273
Identity = 475/609 (78.00%), Postives = 532/609 (87.36%), Query Frame = 0

Query: 1   MLSPIVSKIPLNTAFLLSNSRFPI-AAALRSLSSGSAHRSP---PPHSTMIPPLDSQLPL 60
           M S  +SKI  N+AFLLSN RF I  AALRSL+  S HRSP   PP   M+  +DS+ PL
Sbjct: 1   MRSLFISKISPNSAFLLSNFRFRIPIAALRSLTFSSFHRSPPPMPPRMAMVSHVDSEGPL 60

Query: 61  PQRLWNNCRRESILSMYTPFCLALASGTLHIDAFRRYIDQDVHFLNAFGRAYELAAECAD 120
            +RLWN CRRESI SMYTPFC+ LA GTL +D FR  +DQDVHFL AF +AYELAAECAD
Sbjct: 61  ARRLWNKCRRESIFSMYTPFCVCLACGTLDVDTFRHVVDQDVHFLKAFAQAYELAAECAD 120

Query: 121 DDEARHSINELRNAVTEELKMHASFVKEWGAEEGIESHVSPATLKYTEFLLATASGKIEG 180
           DD+A+HSINELR AV  +LK HASFVKEW AE G ES ++PAT+KYT+F+LATA GKIEG
Sbjct: 121 DDDAKHSINELRKAVMVDLKRHASFVKEWAAEGGKESPINPATVKYTDFVLATAFGKIEG 180

Query: 181 SEGLGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYGDHPYKTWIENYAS 240
            +GLGNLATPFERTKLAAYTLGA+TPCMRLYAYLATEFKGALGAD+G+H YKTWIENYAS
Sbjct: 181 DKGLGNLATPFERTKLAAYTLGAITPCMRLYAYLATEFKGALGADHGNHLYKTWIENYAS 240

Query: 241 KGFEEAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFFYSQPVPHKTVVPLIKD 300
           K FEE+A RTED+LEKLSATLTGEELDTIEKLYHQAMKLE EF+ SQPV  KTVVPLIKD
Sbjct: 241 KDFEESAARTEDILEKLSATLTGEELDTIEKLYHQAMKLEQEFYCSQPVSQKTVVPLIKD 300

Query: 301 HNPAEDRLVFFSDFDLTCTMVDSSAILAELAIIRAPKLDQNQPSEDQVTRMSSADLKKSW 360
           HNPA +RLV FSDFDLTCT+VDSSAILAE+A++RAPK DQ QP +DQ+TRMSSADL+ +W
Sbjct: 301 HNPATERLVLFSDFDLTCTVVDSSAILAEIAMVRAPKPDQTQP-DDQITRMSSADLRNTW 360

Query: 361 GVISRQYTEEYEDCIKQITPPQTEEFKYETLCTALEQLSDFEKKANSRVIESGVLKNLNF 420
           GVISRQYTEEYE+CI +ITPP TEEFKYE LCTALEQLSDFEK+AN+RV+ESGVLK LNF
Sbjct: 361 GVISRQYTEEYEECIDKITPPATEEFKYEDLCTALEQLSDFEKRANNRVVESGVLKGLNF 420

Query: 421 EDIRRAGEHLLIQDGCFNFFGTANKNENLNVAINIVSYCWCGDFIRSSLKSGGLLNQVTL 480
           EDIRRAGE L+IQDGCFNFF TA K+ENLNV ++I+SYCWC D IRSS  SGGLL QVT+
Sbjct: 421 EDIRRAGERLIIQDGCFNFFRTATKSENLNVGVHILSYCWCADLIRSSFNSGGLLTQVTI 480

Query: 481 HANEFIFEESVSTGDLVRKVESPIEKVDVFRKTLESYDNDRKNFTIYVGDSIGDLLCLLE 540
           HANEF FEE+VSTGDLVRKVESPI+KV+ FRK LE+Y NDRKN T+YVGDSIGDLLCLLE
Sbjct: 481 HANEFAFEEAVSTGDLVRKVESPIDKVNSFRKILENYGNDRKNLTVYVGDSIGDLLCLLE 540

Query: 541 ADVGIVIGSSSSLKRLATQFGVSFVPLYQQVVRKQKALTEETRCSWKGLPGVLYTVNTWA 600
           ADVGIV+G+SSSL+RLATQFGVSFVPLY  VVRKQK+LTE+T CSWKGL GVLYTVN+WA
Sbjct: 541 ADVGIVVGTSSSLRRLATQFGVSFVPLYPSVVRKQKSLTEDTECSWKGLSGVLYTVNSWA 600

Query: 601 EIHAFVLGC 606
           EIHAFVLGC
Sbjct: 601 EIHAFVLGC 608

BLAST of Sed0005304 vs. NCBI nr
Match: XP_022988051.1 (bifunctional TH2 protein, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 951.8 bits (2459), Expect = 2.8e-273
Identity = 474/609 (77.83%), Postives = 530/609 (87.03%), Query Frame = 0

Query: 1   MLSPIVSKIPLNTAFLLSNSRFPI-AAALRSLSSGSAHRSP---PPHSTMIPPLDSQLPL 60
           M S  +SKI  N+AFLLSN RF I  AALRSL+  S HRSP   PP   M+  +DS  PL
Sbjct: 1   MRSLFISKISPNSAFLLSNFRFRIPIAALRSLTFSSFHRSPPPMPPRMAMVSHVDSDGPL 60

Query: 61  PQRLWNNCRRESILSMYTPFCLALASGTLHIDAFRRYIDQDVHFLNAFGRAYELAAECAD 120
            +RLWN CRRESI SMYTPFC+ LA GTL +D FR  +DQDVHFL AF +AYELAAECAD
Sbjct: 61  ARRLWNKCRRESIFSMYTPFCVCLACGTLDVDTFRHVVDQDVHFLKAFAQAYELAAECAD 120

Query: 121 DDEARHSINELRNAVTEELKMHASFVKEWGAEEGIESHVSPATLKYTEFLLATASGKIEG 180
           DD+A+HSINELR AV  +LK HASFVKEW AE G ES ++PAT+KYT+F+LATA GKIEG
Sbjct: 121 DDDAKHSINELRKAVMVDLKRHASFVKEWAAEGGKESPINPATVKYTDFVLATAFGKIEG 180

Query: 181 SEGLGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYGDHPYKTWIENYAS 240
            +GLGNLATPFERTKLAAYTLGA+TPCMRLYAYLATEFKGALGAD+G+H YKTWIENYAS
Sbjct: 181 DKGLGNLATPFERTKLAAYTLGAITPCMRLYAYLATEFKGALGADHGNHLYKTWIENYAS 240

Query: 241 KGFEEAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFFYSQPVPHKTVVPLIKD 300
           K FEE+A RTED+LEKLSATLTGEELDTIEKLYHQAMKLE EF+ SQ V  KTVVPLIKD
Sbjct: 241 KDFEESAARTEDILEKLSATLTGEELDTIEKLYHQAMKLEQEFYCSQTVSQKTVVPLIKD 300

Query: 301 HNPAEDRLVFFSDFDLTCTMVDSSAILAELAIIRAPKLDQNQPSEDQVTRMSSADLKKSW 360
           HNPA DRLV FSDFDLTCT+VDSSAILAE+A++RAPK DQ QP +DQ+TRMSSADL+ +W
Sbjct: 301 HNPATDRLVLFSDFDLTCTVVDSSAILAEIAMVRAPKPDQTQP-DDQITRMSSADLRNTW 360

Query: 361 GVISRQYTEEYEDCIKQITPPQTEEFKYETLCTALEQLSDFEKKANSRVIESGVLKNLNF 420
           GVISRQYTEEYE+CI +ITPP TEEFKYE LCTALEQLSDFEK+AN+RV+ESGVLK LNF
Sbjct: 361 GVISRQYTEEYEECIDKITPPATEEFKYEDLCTALEQLSDFEKRANNRVVESGVLKGLNF 420

Query: 421 EDIRRAGEHLLIQDGCFNFFGTANKNENLNVAINIVSYCWCGDFIRSSLKSGGLLNQVTL 480
           EDIRRAGE L+IQDGCFNFF TA K+ENLNV ++I+SYCWC D IRSS  SGGLL QVT+
Sbjct: 421 EDIRRAGERLIIQDGCFNFFRTATKSENLNVGVHILSYCWCADLIRSSFNSGGLLTQVTI 480

Query: 481 HANEFIFEESVSTGDLVRKVESPIEKVDVFRKTLESYDNDRKNFTIYVGDSIGDLLCLLE 540
           HANEF FEE+VSTGDLVRKVESP++KV+ FRK LE+Y NDRKN T+Y+GDSIGDLLCLLE
Sbjct: 481 HANEFAFEEAVSTGDLVRKVESPVDKVNAFRKILENYGNDRKNLTVYIGDSIGDLLCLLE 540

Query: 541 ADVGIVIGSSSSLKRLATQFGVSFVPLYQQVVRKQKALTEETRCSWKGLPGVLYTVNTWA 600
           ADVGIV+G+SSSL+RLATQFGVSFVPLY  VVRKQK+LTEET CSWKGL GVLYTVN+WA
Sbjct: 541 ADVGIVVGTSSSLRRLATQFGVSFVPLYPSVVRKQKSLTEETECSWKGLSGVLYTVNSWA 600

Query: 601 EIHAFVLGC 606
           EIHAFVLGC
Sbjct: 601 EIHAFVLGC 608

BLAST of Sed0005304 vs. ExPASy Swiss-Prot
Match: F4KFT7 (Bifunctional TH2 protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=TH2 PE=1 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 4.3e-192
Identity = 337/566 (59.54%), Postives = 438/566 (77.39%), Query Frame = 0

Query: 44  STMIPPLDSQLPLPQRLWNNCRRESILSMYTPFCLALASGTLHIDAFRRYIDQDVHFLNA 103
           S  IPP  S+  LP +LW    RE + S+Y+PF + LA+G L ID FR+YI QDVHFL A
Sbjct: 55  SLSIPP-TSEEALPGKLWIKFNRECLFSIYSPFAVCLAAGNLKIDTFRQYIAQDVHFLKA 114

Query: 104 FGRAYELAAECADDDEARHSINELRNAVTEELKMHASFVKEWGAEEGIESHVSPATLKYT 163
           F  AYELAA+CADDD+ + +I++LR +V EELKMH SFV++W  +   E  V+ ATL+YT
Sbjct: 115 FAHAYELAADCADDDDDKLAISDLRKSVMEELKMHDSFVQDWDLDINKEVSVNSATLRYT 174

Query: 164 EFLLATASGKIEGSEGLGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYG 223
           EFLLATASGK+EG +  G L TPFE+TK+AAYTLGA+TPCMRLYA+L  EF   L     
Sbjct: 175 EFLLATASGKVEGCKAPGMLDTPFEKTKVAAYTLGAVTPCMRLYAFLGKEFGSLLDLSDV 234

Query: 224 DHPYKTWIENYASKGFEEAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFFYSQ 283
           +HPYK WI+NY+S  F+ +A++TED+LEKLS ++TGEELD IEKLY QAMKLE+EFF++Q
Sbjct: 235 NHPYKKWIDNYSSDAFQASAKQTEDLLEKLSVSMTGEELDIIEKLYQQAMKLEVEFFHAQ 294

Query: 284 PVPHKTVVPLIKDHNPAEDRLVFFSDFDLTCTMVDSSAILAELAIIRAPKLDQNQPSEDQ 343
           P+   T+VPL+K+H  ++D LV FSDFDLTCT+VDSSAILAE+AI+ APK D+   S  Q
Sbjct: 295 PLAQPTIVPLLKNH--SKDDLVIFSDFDLTCTVVDSSAILAEIAIVTAPK-DEQSRSGQQ 354

Query: 344 VTRMSSADLKKSWGVISRQYTEEYEDCIKQI-TPPQTEEFKYETLCTALEQLSDFEKKAN 403
           + RM S+DLK +W ++S+QYTE YE+CI+ I    + ++F YE LC ALEQLSDFEK+AN
Sbjct: 355 IHRMLSSDLKNTWNLLSKQYTEHYEECIESILNKKKADKFDYEGLCKALEQLSDFEKEAN 414

Query: 404 SRVIESGVLKNLNFEDIRRAGEHLLIQDGCFNFFGTANKNENLNVAINIVSYCWCGDFIR 463
           +RVIESGVLK LN EDI+RAGE L++QDGC N F    K ENLN  ++++SYCWCGD IR
Sbjct: 415 NRVIESGVLKGLNLEDIKRAGERLILQDGCINVFQKILKTENLNAELHVLSYCWCGDLIR 474

Query: 464 SSLKSGGLLNQVTLHANEFIFEESVSTGDLVRKVESPIEKVDVFRKTLES--YDNDRKNF 523
           ++  +GG ++ V +HANEF FEES+STG++ RKVESPI K   F+  L++   +N++K+F
Sbjct: 475 AAFSAGG-VDAVEVHANEFTFEESISTGEIERKVESPINKAQQFKSILQNRKNENNKKSF 534

Query: 524 -TIYVGDSIGDLLCLLEADVGIVIGSSSSLKRLATQFGVSFVPLYQQVVRKQKALTEETR 583
            ++Y+GDS+GDLLCLLEAD+GIV+ SSSSL+R+ + FGVSFVPL+  +V+KQK  TEE+ 
Sbjct: 535 LSVYIGDSVGDLLCLLEADIGIVVSSSSSLRRVGSHFGVSFVPLFSGIVQKQKQHTEESS 594

Query: 584 CS-WKGLPGVLYTVNTWAEIHAFVLG 605
            S WKGL G LYTV++WAEIH+F LG
Sbjct: 595 SSAWKGLSGTLYTVSSWAEIHSFALG 615

BLAST of Sed0005304 vs. ExPASy Swiss-Prot
Match: P44659 (Uncharacterized protein HI_0358 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=HI_0358 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.3e-14
Identity = 60/210 (28.57%), Postives = 102/210 (48.57%), Query Frame = 0

Query: 76  FCLALASGTLHIDAFRRYIDQDVHFLNAFGRAYELAAECADDDEARHSINELRNAVTEEL 135
           F   LA GTL    F+ Y+ QD  +L  + RA+ L    A +     +  +    + +E+
Sbjct: 21  FVQQLAKGTLPKACFQHYLKQDYLYLFHYSRAFALGVFKAKNFAEMETPRKTLEILCQEI 80

Query: 136 KMHASFVKEWG--AEEGIESHVSPATLKYTEFLLATASGKIEGSEGLGNLATPFERTKLA 195
           ++H ++ +EWG   +E   +  S A + YT +LL             G+LA  +      
Sbjct: 81  QLHLNYCREWGISEQEIFTTQESAACIAYTRYLLDCGM--------TGSLAELY------ 140

Query: 196 AYTLGAMTPCMRLYAYLA---TEFKGALGADYGDHPYKTWIENYASKGFEEAAERTEDVL 255
                A+TPC   YA +A   T+    L     ++PY+TWI+ YAS+ F++AA+ T D L
Sbjct: 141 ----AAVTPCALGYAQVARYITQHYPRL----PNNPYQTWIDTYASEEFQQAAQETVDFL 200

Query: 256 EKLSATLTGEELDTIEKLYHQAMKLELEFF 281
             L   L   +L  I++++  A ++E+ F+
Sbjct: 201 TALCKPLNPSQLAEIQQIFTTATRMEIAFW 208

BLAST of Sed0005304 vs. ExPASy Swiss-Prot
Match: Q8FTH8 (Thiamine biosynthesis multifunctional protein ThiED OS=Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) OX=196164 GN=thiED PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 6.8e-12
Identity = 67/255 (26.27%), Postives = 104/255 (40.78%), Query Frame = 0

Query: 38  RSPPPHSTMIPPLDSQLPLP---------QRLWNNCRRESILSMYTPFCLALASGTLHID 97
           R   P   +I P D+Q P P         + LW          + + F   L  GTL  +
Sbjct: 500 RMGAPSDGIITPSDTQSPAPALAPAGPYTRALWEATGDVLGEILDSGFIRGLGDGTLSRE 559

Query: 98  AFRRYIDQDVHFLNAFGRAYELAAECADDDEARHS-INELRNAVTEELKMHASFVKEWGA 157
            F  YIDQD H+L  + RA    +  A D  A+          +T E ++H +++ +  A
Sbjct: 560 EFLFYIDQDAHYLRQYSRALATLSSRAPDAPAQVDWATSAAECITVEAELHRTYLNKGLA 619

Query: 158 EEGIESHVSPATLKYTEFLLATASGKIEGSEGLGNLATPFERTKLAAYTLG--AMTPCMR 217
           E G+ S  SP T+ YT+FL+A                    R+    Y +G  A+ PC  
Sbjct: 620 ETGV-SAPSPVTMAYTDFLIA--------------------RSHADDYVVGAAAVLPCYW 679

Query: 218 LYAYLATEFKGALGADYGDHPYKTWIENYASKGFEEAAERTEDVLEKLSATLTGEELDTI 277
           LYA +          ++ +HPY  W++ Y+ +GF     +    +E   A    ++    
Sbjct: 680 LYAEIGLIL---AKQNHPEHPYTDWLDTYSGEGFLAGTVKAIARVEAAMAGAGPDQQRVA 730

Query: 278 EKLYHQAMKLELEFF 281
            + Y  A   E EFF
Sbjct: 740 AQTYLSACVHEREFF 730

BLAST of Sed0005304 vs. ExPASy Swiss-Prot
Match: Q08224 (Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=THI20 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.3e-07
Identity = 55/212 (25.94%), Postives = 88/212 (41.51%), Query Frame = 0

Query: 76  FCLALASGTLHIDAFRRYIDQDVHFLNAFGRAYELA---AECADDDEARHSINELRNAVT 135
           F   +A GTL    F+ +I+QD  +L  + R + +A   A C +D E    I      V 
Sbjct: 355 FVKKVADGTLERKKFQFFIEQDYAYLVDYARVHCIAGSKAPCLEDMEKELVI---VGGVR 414

Query: 136 EELKMHASFVKE-WGAEEG---IESHVSPATLKYTEFLLATASGKIEGSEGLGNLATPFE 195
            E+  H   +KE +G ++     +    PA   Y+ +                       
Sbjct: 415 TEMGQHEKRLKEVFGVKDPDYFQKIKRGPALRAYSRYF------------------NDVS 474

Query: 196 RTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYGDHPYKTWIENYASKGFEEAAERTED 255
           R       + ++TPC+  Y    T+ KG + A  G   Y  W E YAS  + EA +  E 
Sbjct: 475 RRGNWQELVASLTPCLMGYGEALTKMKGKVTAPEGS-VYHEWCETYASSWYREAMDEGEK 534

Query: 256 VLEKLSATLTGEELDTIEKLYHQAMKLELEFF 281
           +L  +  T   E+LDT+  +Y +  +LE  F+
Sbjct: 535 LLNHILETYPPEQLDTLVTIYAEVCELETNFW 544

BLAST of Sed0005304 vs. ExPASy Swiss-Prot
Match: O94266 (Putative hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBP8B7.18c PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 7.3e-06
Identity = 55/216 (25.46%), Postives = 92/216 (42.59%), Query Frame = 0

Query: 76  FCLALASGTLHIDAFRRYIDQDVHFLNAFGRAYELAAECADDD----EARHSINELRNAV 135
           F   LA GTL + AF+ Y+ QD  +L  F RAY L     +      EA  S+      V
Sbjct: 345 FTNMLAKGTLPLPAFQDYLKQDYLYLVNFARAYSLKGYKENTFPNILEAAQSVIH----V 404

Query: 136 TEELKMHASFVKEWGAE-EGIES-HVSPATLKYTEFLLATASGKIEGSEGLGNLATPFER 195
            EE ++H S    +G   + ++S   SPA   Y+ ++L T + +                
Sbjct: 405 IEEKELHVSMCSSYGVSLQDLKSCEESPACTAYSRYILDTGAAQ---------------- 464

Query: 196 TKLAAYTLGAMTPCMRLYAYLATE-----FKGALGADYGDHPYKTWIENYASKGFEEAAE 255
             +AA       PC+  Y  +A       F+   G      PY+ W++NY  + +  A  
Sbjct: 465 -DVAALDF-VQAPCLIGYYVIAARLMKEPFRNPQG------PYQKWVDNYFCEDYLSAVR 524

Query: 256 RTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFF 281
           R    +E++   L+ E +  + +++ +A K E  F+
Sbjct: 525 RGCRQIEEIVLKLSPERIQELIEIFIRATKFETLFW 532

BLAST of Sed0005304 vs. ExPASy TrEMBL
Match: A0A5A7T9M1 (Putative aminopyrimidine aminohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold506G001380 PE=4 SV=1)

HSP 1 Score: 956.4 bits (2471), Expect = 5.5e-275
Identity = 476/616 (77.27%), Postives = 531/616 (86.20%), Query Frame = 0

Query: 1   MLSPIVSKIPLNTAFLLSNSRFP-IAAALRSLSSGSAHRSP----------PPHSTMIPP 60
           M +  + K PL++ F+  NS FP +  A RSLS  S HRSP          PP   MI  
Sbjct: 1   MRTLFIPKFPLSSLFVFPNSPFPFLIDAFRSLSFHSLHRSPSSAASSSPTMPPRLAMISH 60

Query: 61  LDSQLPLPQRLWNNCRRESILSMYTPFCLALASGTLHIDAFRRYIDQDVHFLNAFGRAYE 120
           +DS  PL +RLWN CRRESILSMYTPFC+ LA GTL+ID FR YIDQDVHFL AF RAYE
Sbjct: 61  VDSDGPLARRLWNKCRRESILSMYTPFCVCLACGTLNIDTFRHYIDQDVHFLKAFARAYE 120

Query: 121 LAAECADDDEARHSINELRNAVTEELKMHASFVKEWGAEEGIESHVSPATLKYTEFLLAT 180
           LAAECADDD+A+HSINELR AV+ ELKMHASFVKEW A +G ES V+PAT+KYT+FLLAT
Sbjct: 121 LAAECADDDDAKHSINELRKAVSVELKMHASFVKEWAAADGKESPVNPATIKYTDFLLAT 180

Query: 181 ASGKIEGSEGLGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYGDHPYKT 240
           ASGKIEG+EGLGNLATPFERTKLAAY LGAMTPCMRLYAYLATEFKG LGA +GDHPYKT
Sbjct: 181 ASGKIEGAEGLGNLATPFERTKLAAYALGAMTPCMRLYAYLATEFKGVLGALHGDHPYKT 240

Query: 241 WIENYASKGFEEAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFFYSQPVPHKT 300
           WIENYASKGFEEAAE+TEDVLEKLSATLTGEELDTIEKLYHQAMKLE EFF SQPV  KT
Sbjct: 241 WIENYASKGFEEAAEKTEDVLEKLSATLTGEELDTIEKLYHQAMKLEQEFFCSQPVSQKT 300

Query: 301 VVPLIKDHNPAEDRLVFFSDFDLTCTMVDSSAILAELAIIRAPKLDQNQPSEDQVTRMSS 360
           V+PLIKDHNPAEDRLV FSDFDLTCT+VDSSAILAE+AI+RAPK DQ QP +   TRMSS
Sbjct: 301 VLPLIKDHNPAEDRLVLFSDFDLTCTVVDSSAILAEIAIVRAPKPDQIQPEDQPFTRMSS 360

Query: 361 ADLKKSWGVISRQYTEEYEDCIKQITPPQTEEFKYETLCTALEQLSDFEKKANSRVIESG 420
           ADL+ +WGVISRQYTEEYE+CI ++ PP+T EFK+E LCTALEQLSDFEK+AN+RV+ESG
Sbjct: 361 ADLRNTWGVISRQYTEEYEECIDKVMPPKTVEFKFEDLCTALEQLSDFEKRANNRVVESG 420

Query: 421 VLKNLNFEDIRRAGEHLLIQDGCFNFFGTANKNENLNVAINIVSYCWCGDFIRSSLKSGG 480
           VLK LNFEDIRRAGEHL+IQDGCFNFFGTA K+ENLNV ++I+SYCWC D IRSS  SGG
Sbjct: 421 VLKGLNFEDIRRAGEHLIIQDGCFNFFGTACKSENLNVGVHILSYCWCADLIRSSFNSGG 480

Query: 481 LLNQVTLHANEFIFEESVSTGDLVRKVESPIEKVDVFRKTLESYDNDRKNFTIYVGDSIG 540
           LL QVT+HANEF FEE+VSTGDLVR+VESP++KV+ FRK LE+Y NDR N T+Y+GDSIG
Sbjct: 481 LLTQVTIHANEFAFEEAVSTGDLVRRVESPLDKVNAFRKILENYGNDRNNLTVYIGDSIG 540

Query: 541 DLLCLLEADVGIVIGSSSSLKRLATQFGVSFVPLYQQVVRKQKALTEETRCSWKGLPGVL 600
           DLLCLLEAD+GIVIGSS+SL+RLATQFGVSFVPLY  VVRKQK LTE++R SWKGL GVL
Sbjct: 541 DLLCLLEADIGIVIGSSASLRRLATQFGVSFVPLYPSVVRKQKDLTEDSRRSWKGLSGVL 600

Query: 601 YTVNTWAEIHAFVLGC 606
           YTVN+WAEIHAFVLGC
Sbjct: 601 YTVNSWAEIHAFVLGC 616

BLAST of Sed0005304 vs. ExPASy TrEMBL
Match: A0A1S3B8T0 (probable aminopyrimidine aminohydrolase, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487252 PE=4 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.6e-274
Identity = 475/616 (77.11%), Postives = 531/616 (86.20%), Query Frame = 0

Query: 1   MLSPIVSKIPLNTAFLLSNSRFP-IAAALRSLSSGSAHRSP----------PPHSTMIPP 60
           M +  + K PL++ F+  NS FP +  A RSLS  S HRSP          PP   MI  
Sbjct: 1   MRTLFIPKFPLSSLFVFPNSPFPFLIDAFRSLSFHSLHRSPSSAASSSPTMPPRLAMISH 60

Query: 61  LDSQLPLPQRLWNNCRRESILSMYTPFCLALASGTLHIDAFRRYIDQDVHFLNAFGRAYE 120
           +DS  PL +RLWN CRRESILSMYTPFC+ LA GTL+ID FR YIDQDVHFL AF RAYE
Sbjct: 61  VDSDGPLARRLWNKCRRESILSMYTPFCVCLACGTLNIDTFRHYIDQDVHFLKAFARAYE 120

Query: 121 LAAECADDDEARHSINELRNAVTEELKMHASFVKEWGAEEGIESHVSPATLKYTEFLLAT 180
           LAAECADDD+A+HSINELR AV+ ELKMHASFVKEW A +G ES V+PAT+KYT+FLLAT
Sbjct: 121 LAAECADDDDAKHSINELRKAVSVELKMHASFVKEWAAADGKESPVNPATIKYTDFLLAT 180

Query: 181 ASGKIEGSEGLGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYGDHPYKT 240
           ASGKIEG+EGLGNLATPFERTKLAAY LGAMTPCMRLYAYLATEFKG LGA +GDHPYKT
Sbjct: 181 ASGKIEGAEGLGNLATPFERTKLAAYALGAMTPCMRLYAYLATEFKGVLGALHGDHPYKT 240

Query: 241 WIENYASKGFEEAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFFYSQPVPHKT 300
           WIENYASKGFEEAAE+TEDVLEKLSATLTGEELDTIEKLYHQAMKLE EFF SQPV  KT
Sbjct: 241 WIENYASKGFEEAAEKTEDVLEKLSATLTGEELDTIEKLYHQAMKLEQEFFCSQPVSQKT 300

Query: 301 VVPLIKDHNPAEDRLVFFSDFDLTCTMVDSSAILAELAIIRAPKLDQNQPSEDQVTRMSS 360
           V+PLIKDHNPAEDRLV FSDFDLTCT+VDSSAILAE+AI+RAPK DQ QP +   TRMSS
Sbjct: 301 VLPLIKDHNPAEDRLVLFSDFDLTCTVVDSSAILAEIAIVRAPKPDQIQPEDQPFTRMSS 360

Query: 361 ADLKKSWGVISRQYTEEYEDCIKQITPPQTEEFKYETLCTALEQLSDFEKKANSRVIESG 420
           ADL+ +WGVISRQYTEEYE+CI ++ PP+T EFK+E LCTALEQLSDFEK+AN+RV+ESG
Sbjct: 361 ADLRNTWGVISRQYTEEYEECIDKVMPPKTVEFKFEDLCTALEQLSDFEKRANNRVVESG 420

Query: 421 VLKNLNFEDIRRAGEHLLIQDGCFNFFGTANKNENLNVAINIVSYCWCGDFIRSSLKSGG 480
           VLK LNFEDIRRAGEHL+IQDGCFNFFGTA K+ENLNV ++I+SYCWC D IRSS  SGG
Sbjct: 421 VLKGLNFEDIRRAGEHLIIQDGCFNFFGTACKSENLNVGVHILSYCWCADLIRSSFNSGG 480

Query: 481 LLNQVTLHANEFIFEESVSTGDLVRKVESPIEKVDVFRKTLESYDNDRKNFTIYVGDSIG 540
           LL QVT+HANEF FEE+VSTGDLVR+VESP++KV+ FRK LE+Y NDR N T+Y+GDSIG
Sbjct: 481 LLTQVTIHANEFAFEEAVSTGDLVRRVESPLDKVNAFRKILENYGNDRNNLTVYIGDSIG 540

Query: 541 DLLCLLEADVGIVIGSSSSLKRLATQFGVSFVPLYQQVVRKQKALTEETRCSWKGLPGVL 600
           DLLCLLEAD+GIVIGSS+SL+RLAT+FGVSFVPLY  VVRKQK LTE++R SWKGL GVL
Sbjct: 541 DLLCLLEADIGIVIGSSASLRRLATRFGVSFVPLYPSVVRKQKDLTEDSRRSWKGLSGVL 600

Query: 601 YTVNTWAEIHAFVLGC 606
           YTVN+WAEIHAFVLGC
Sbjct: 601 YTVNSWAEIHAFVLGC 616

BLAST of Sed0005304 vs. ExPASy TrEMBL
Match: A0A6J1JG45 (bifunctional TH2 protein, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111485408 PE=4 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 1.4e-273
Identity = 474/609 (77.83%), Postives = 530/609 (87.03%), Query Frame = 0

Query: 1   MLSPIVSKIPLNTAFLLSNSRFPI-AAALRSLSSGSAHRSP---PPHSTMIPPLDSQLPL 60
           M S  +SKI  N+AFLLSN RF I  AALRSL+  S HRSP   PP   M+  +DS  PL
Sbjct: 1   MRSLFISKISPNSAFLLSNFRFRIPIAALRSLTFSSFHRSPPPMPPRMAMVSHVDSDGPL 60

Query: 61  PQRLWNNCRRESILSMYTPFCLALASGTLHIDAFRRYIDQDVHFLNAFGRAYELAAECAD 120
            +RLWN CRRESI SMYTPFC+ LA GTL +D FR  +DQDVHFL AF +AYELAAECAD
Sbjct: 61  ARRLWNKCRRESIFSMYTPFCVCLACGTLDVDTFRHVVDQDVHFLKAFAQAYELAAECAD 120

Query: 121 DDEARHSINELRNAVTEELKMHASFVKEWGAEEGIESHVSPATLKYTEFLLATASGKIEG 180
           DD+A+HSINELR AV  +LK HASFVKEW AE G ES ++PAT+KYT+F+LATA GKIEG
Sbjct: 121 DDDAKHSINELRKAVMVDLKRHASFVKEWAAEGGKESPINPATVKYTDFVLATAFGKIEG 180

Query: 181 SEGLGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYGDHPYKTWIENYAS 240
            +GLGNLATPFERTKLAAYTLGA+TPCMRLYAYLATEFKGALGAD+G+H YKTWIENYAS
Sbjct: 181 DKGLGNLATPFERTKLAAYTLGAITPCMRLYAYLATEFKGALGADHGNHLYKTWIENYAS 240

Query: 241 KGFEEAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFFYSQPVPHKTVVPLIKD 300
           K FEE+A RTED+LEKLSATLTGEELDTIEKLYHQAMKLE EF+ SQ V  KTVVPLIKD
Sbjct: 241 KDFEESAARTEDILEKLSATLTGEELDTIEKLYHQAMKLEQEFYCSQTVSQKTVVPLIKD 300

Query: 301 HNPAEDRLVFFSDFDLTCTMVDSSAILAELAIIRAPKLDQNQPSEDQVTRMSSADLKKSW 360
           HNPA DRLV FSDFDLTCT+VDSSAILAE+A++RAPK DQ QP +DQ+TRMSSADL+ +W
Sbjct: 301 HNPATDRLVLFSDFDLTCTVVDSSAILAEIAMVRAPKPDQTQP-DDQITRMSSADLRNTW 360

Query: 361 GVISRQYTEEYEDCIKQITPPQTEEFKYETLCTALEQLSDFEKKANSRVIESGVLKNLNF 420
           GVISRQYTEEYE+CI +ITPP TEEFKYE LCTALEQLSDFEK+AN+RV+ESGVLK LNF
Sbjct: 361 GVISRQYTEEYEECIDKITPPATEEFKYEDLCTALEQLSDFEKRANNRVVESGVLKGLNF 420

Query: 421 EDIRRAGEHLLIQDGCFNFFGTANKNENLNVAINIVSYCWCGDFIRSSLKSGGLLNQVTL 480
           EDIRRAGE L+IQDGCFNFF TA K+ENLNV ++I+SYCWC D IRSS  SGGLL QVT+
Sbjct: 421 EDIRRAGERLIIQDGCFNFFRTATKSENLNVGVHILSYCWCADLIRSSFNSGGLLTQVTI 480

Query: 481 HANEFIFEESVSTGDLVRKVESPIEKVDVFRKTLESYDNDRKNFTIYVGDSIGDLLCLLE 540
           HANEF FEE+VSTGDLVRKVESP++KV+ FRK LE+Y NDRKN T+Y+GDSIGDLLCLLE
Sbjct: 481 HANEFAFEEAVSTGDLVRKVESPVDKVNAFRKILENYGNDRKNLTVYIGDSIGDLLCLLE 540

Query: 541 ADVGIVIGSSSSLKRLATQFGVSFVPLYQQVVRKQKALTEETRCSWKGLPGVLYTVNTWA 600
           ADVGIV+G+SSSL+RLATQFGVSFVPLY  VVRKQK+LTEET CSWKGL GVLYTVN+WA
Sbjct: 541 ADVGIVVGTSSSLRRLATQFGVSFVPLYPSVVRKQKSLTEETECSWKGLSGVLYTVNSWA 600

Query: 601 EIHAFVLGC 606
           EIHAFVLGC
Sbjct: 601 EIHAFVLGC 608

BLAST of Sed0005304 vs. ExPASy TrEMBL
Match: A0A0A0LY72 (TENA_THI-4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G665890 PE=4 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 2.3e-273
Identity = 474/617 (76.82%), Postives = 530/617 (85.90%), Query Frame = 0

Query: 1   MLSPIVSKIPLNTAFLLSNSRFPI-AAALRSLSSGSAHRSP-----------PPHSTMIP 60
           M +  + K PLN+ FL SNS FP   AA RSLS  S HRSP           PP   MI 
Sbjct: 1   MRTLFIPKFPLNSLFLFSNSPFPFPIAAFRSLSFHSFHRSPSSAASSSSPTMPPRLAMIS 60

Query: 61  PLDSQLPLPQRLWNNCRRESILSMYTPFCLALASGTLHIDAFRRYIDQDVHFLNAFGRAY 120
            +DS  PL +RLWN CRRESILSMYTPFC+ LA GTL+ID FR YI QDVHFL AF RAY
Sbjct: 61  HVDSGGPLARRLWNKCRRESILSMYTPFCVCLACGTLNIDTFRHYIGQDVHFLKAFARAY 120

Query: 121 ELAAECADDDEARHSINELRNAVTEELKMHASFVKEWGAEEGIESHVSPATLKYTEFLLA 180
           ELAAECADDD+A+HSINELR AV+EELKMHASFVKEW A +G ES V+PAT+KYT+FLLA
Sbjct: 121 ELAAECADDDDAKHSINELRKAVSEELKMHASFVKEWTAADGKESPVNPATVKYTDFLLA 180

Query: 181 TASGKIEGSEGLGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYGDHPYK 240
           TASGKIEG+EGL NLATPFERTKLAAY LGAMTPCMRLYAYLA EFKG LGA +GDHPYK
Sbjct: 181 TASGKIEGAEGLANLATPFERTKLAAYALGAMTPCMRLYAYLAKEFKGVLGALHGDHPYK 240

Query: 241 TWIENYASKGFEEAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFFYSQPVPHK 300
           TWIENYASKGFEEAAERTEDVLEKL+ATLTGEELDTIEKLYHQAMKLE EFF SQPV  K
Sbjct: 241 TWIENYASKGFEEAAERTEDVLEKLAATLTGEELDTIEKLYHQAMKLEQEFFCSQPVSQK 300

Query: 301 TVVPLIKDHNPAEDRLVFFSDFDLTCTMVDSSAILAELAIIRAPKLDQNQPSEDQVTRMS 360
           TV+PLIKDHNPAEDRLV FSDFDLTCT+VDSSAILAE+AI+RAPK +Q QP +  +TRMS
Sbjct: 301 TVLPLIKDHNPAEDRLVLFSDFDLTCTVVDSSAILAEIAIVRAPKPEQIQPEDQPITRMS 360

Query: 361 SADLKKSWGVISRQYTEEYEDCIKQITPPQTEEFKYETLCTALEQLSDFEKKANSRVIES 420
           SADL+ +WGVISRQYTEEYE+CI ++ PP+TEEFK+E LCTALE LSDFEK+AN+RVIES
Sbjct: 361 SADLRNTWGVISRQYTEEYEECIDKVLPPKTEEFKFEDLCTALELLSDFEKRANNRVIES 420

Query: 421 GVLKNLNFEDIRRAGEHLLIQDGCFNFFGTANKNENLNVAINIVSYCWCGDFIRSSLKSG 480
           GVLK LNFEDIRRAGEHL+IQDGCFNFFGTA K+ENLNV ++I+SYCWC D IRSS  SG
Sbjct: 421 GVLKGLNFEDIRRAGEHLIIQDGCFNFFGTACKSENLNVGVHILSYCWCADLIRSSFNSG 480

Query: 481 GLLNQVTLHANEFIFEESVSTGDLVRKVESPIEKVDVFRKTLESYDNDRKNFTIYVGDSI 540
           GLL QVT+HANEF FEE+VSTGDLVR+VESP++KV  FRK LE+Y NDR N T+Y+GDSI
Sbjct: 481 GLLTQVTIHANEFAFEEAVSTGDLVRRVESPLDKVHAFRKVLENYGNDRNNLTVYIGDSI 540

Query: 541 GDLLCLLEADVGIVIGSSSSLKRLATQFGVSFVPLYQQVVRKQKALTEETRCSWKGLPGV 600
           GDLLCLLEAD+GIVIGSS+SL+RLAT+FGVSFVPLY  VVRKQK LT+++R SW+GL G+
Sbjct: 541 GDLLCLLEADIGIVIGSSASLRRLATRFGVSFVPLYPSVVRKQKDLTKDSRRSWRGLSGI 600

Query: 601 LYTVNTWAEIHAFVLGC 606
           LYTVN+WAEIHAFVLGC
Sbjct: 601 LYTVNSWAEIHAFVLGC 617

BLAST of Sed0005304 vs. ExPASy TrEMBL
Match: A0A6J1HD46 (bifunctional TH2 protein, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111461743 PE=4 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 2.6e-272
Identity = 472/609 (77.50%), Postives = 529/609 (86.86%), Query Frame = 0

Query: 1   MLSPIVSKIPLNTAFLLSNSRFPI-AAALRSLSSGSAHRSP---PPHSTMIPPLDSQLPL 60
           M S  +SKI  N+A LLSN RF I  AALRS +  S HRSP   PP   M+  +DS+ PL
Sbjct: 1   MRSLFISKISPNSACLLSNFRFRIPIAALRSFTFSSFHRSPPPMPPRMAMVSHVDSEGPL 60

Query: 61  PQRLWNNCRRESILSMYTPFCLALASGTLHIDAFRRYIDQDVHFLNAFGRAYELAAECAD 120
            +RLWN CRRESI SMYTPFC+ LA GTL +D FR  +DQDVHFL AF +AYELAAECAD
Sbjct: 61  ARRLWNKCRRESIFSMYTPFCVCLACGTLDVDTFRHVVDQDVHFLKAFAQAYELAAECAD 120

Query: 121 DDEARHSINELRNAVTEELKMHASFVKEWGAEEGIESHVSPATLKYTEFLLATASGKIEG 180
           DD+A+HSINELR AV  +LK HASFVKEW AE G ES ++PAT+KYT+F+LATA GKIEG
Sbjct: 121 DDDAKHSINELRKAVMVDLKRHASFVKEWAAEGGKESPINPATVKYTDFVLATALGKIEG 180

Query: 181 SEGLGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYGDHPYKTWIENYAS 240
            +GLGNLATPFERTKLAAYTLGA+TPCMRLYAYLATEFKGALGAD+G+H YKTWIENYAS
Sbjct: 181 DKGLGNLATPFERTKLAAYTLGAITPCMRLYAYLATEFKGALGADHGNHLYKTWIENYAS 240

Query: 241 KGFEEAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFFYSQPVPHKTVVPLIKD 300
           K FEE+A RTED+LEKLSATLTGEELDTIEKLYHQAMKLE EF+ SQPV  KTVVPLIKD
Sbjct: 241 KDFEESAARTEDILEKLSATLTGEELDTIEKLYHQAMKLEQEFYCSQPVSQKTVVPLIKD 300

Query: 301 HNPAEDRLVFFSDFDLTCTMVDSSAILAELAIIRAPKLDQNQPSEDQVTRMSSADLKKSW 360
           HNPA DRLV FSDFDLTCT+VDSSAILAE+A++RAPK DQ QP +DQ+TRMSSADL+ +W
Sbjct: 301 HNPATDRLVLFSDFDLTCTVVDSSAILAEIAMVRAPKPDQTQP-DDQITRMSSADLRNTW 360

Query: 361 GVISRQYTEEYEDCIKQITPPQTEEFKYETLCTALEQLSDFEKKANSRVIESGVLKNLNF 420
           GVISRQYTEEYE+CI +I PP TEEFKYE LCTALEQLSDFEK+AN+RV+ESGVLK LNF
Sbjct: 361 GVISRQYTEEYEECIDKIAPPATEEFKYEDLCTALEQLSDFEKRANNRVVESGVLKGLNF 420

Query: 421 EDIRRAGEHLLIQDGCFNFFGTANKNENLNVAINIVSYCWCGDFIRSSLKSGGLLNQVTL 480
           EDIRRAGE L+IQDGCFNFF TA K+ENLNV ++I+SYCWC D IRSS  SGGLL QVT+
Sbjct: 421 EDIRRAGERLIIQDGCFNFFRTATKSENLNVGVHILSYCWCADLIRSSFNSGGLLTQVTI 480

Query: 481 HANEFIFEESVSTGDLVRKVESPIEKVDVFRKTLESYDNDRKNFTIYVGDSIGDLLCLLE 540
           HANEF FEE+VSTGDLVRKVESPI+KV+ FRK LE+Y NDRKN T+Y+GDSIGDLLCLLE
Sbjct: 481 HANEFAFEEAVSTGDLVRKVESPIDKVNAFRKILENYGNDRKNSTVYIGDSIGDLLCLLE 540

Query: 541 ADVGIVIGSSSSLKRLATQFGVSFVPLYQQVVRKQKALTEETRCSWKGLPGVLYTVNTWA 600
           ADVGIV+G+SSSL+RLATQFGVSFVPLY  VVRKQ++LTEET CSWKGL GVLYTVN+WA
Sbjct: 541 ADVGIVVGTSSSLRRLATQFGVSFVPLYPSVVRKQRSLTEETECSWKGLSGVLYTVNSWA 600

Query: 601 EIHAFVLGC 606
           EIHAFVLGC
Sbjct: 601 EIHAFVLGC 608

BLAST of Sed0005304 vs. TAIR 10
Match: AT5G32470.1 (Haem oxygenase-like, multi-helical )

HSP 1 Score: 672.5 bits (1734), Expect = 3.1e-193
Identity = 337/566 (59.54%), Postives = 438/566 (77.39%), Query Frame = 0

Query: 44  STMIPPLDSQLPLPQRLWNNCRRESILSMYTPFCLALASGTLHIDAFRRYIDQDVHFLNA 103
           S  IPP  S+  LP +LW    RE + S+Y+PF + LA+G L ID FR+YI QDVHFL A
Sbjct: 55  SLSIPP-TSEEALPGKLWIKFNRECLFSIYSPFAVCLAAGNLKIDTFRQYIAQDVHFLKA 114

Query: 104 FGRAYELAAECADDDEARHSINELRNAVTEELKMHASFVKEWGAEEGIESHVSPATLKYT 163
           F  AYELAA+CADDD+ + +I++LR +V EELKMH SFV++W  +   E  V+ ATL+YT
Sbjct: 115 FAHAYELAADCADDDDDKLAISDLRKSVMEELKMHDSFVQDWDLDINKEVSVNSATLRYT 174

Query: 164 EFLLATASGKIEGSEGLGNLATPFERTKLAAYTLGAMTPCMRLYAYLATEFKGALGADYG 223
           EFLLATASGK+EG +  G L TPFE+TK+AAYTLGA+TPCMRLYA+L  EF   L     
Sbjct: 175 EFLLATASGKVEGCKAPGMLDTPFEKTKVAAYTLGAVTPCMRLYAFLGKEFGSLLDLSDV 234

Query: 224 DHPYKTWIENYASKGFEEAAERTEDVLEKLSATLTGEELDTIEKLYHQAMKLELEFFYSQ 283
           +HPYK WI+NY+S  F+ +A++TED+LEKLS ++TGEELD IEKLY QAMKLE+EFF++Q
Sbjct: 235 NHPYKKWIDNYSSDAFQASAKQTEDLLEKLSVSMTGEELDIIEKLYQQAMKLEVEFFHAQ 294

Query: 284 PVPHKTVVPLIKDHNPAEDRLVFFSDFDLTCTMVDSSAILAELAIIRAPKLDQNQPSEDQ 343
           P+   T+VPL+K+H  ++D LV FSDFDLTCT+VDSSAILAE+AI+ APK D+   S  Q
Sbjct: 295 PLAQPTIVPLLKNH--SKDDLVIFSDFDLTCTVVDSSAILAEIAIVTAPK-DEQSRSGQQ 354

Query: 344 VTRMSSADLKKSWGVISRQYTEEYEDCIKQI-TPPQTEEFKYETLCTALEQLSDFEKKAN 403
           + RM S+DLK +W ++S+QYTE YE+CI+ I    + ++F YE LC ALEQLSDFEK+AN
Sbjct: 355 IHRMLSSDLKNTWNLLSKQYTEHYEECIESILNKKKADKFDYEGLCKALEQLSDFEKEAN 414

Query: 404 SRVIESGVLKNLNFEDIRRAGEHLLIQDGCFNFFGTANKNENLNVAINIVSYCWCGDFIR 463
           +RVIESGVLK LN EDI+RAGE L++QDGC N F    K ENLN  ++++SYCWCGD IR
Sbjct: 415 NRVIESGVLKGLNLEDIKRAGERLILQDGCINVFQKILKTENLNAELHVLSYCWCGDLIR 474

Query: 464 SSLKSGGLLNQVTLHANEFIFEESVSTGDLVRKVESPIEKVDVFRKTLES--YDNDRKNF 523
           ++  +GG ++ V +HANEF FEES+STG++ RKVESPI K   F+  L++   +N++K+F
Sbjct: 475 AAFSAGG-VDAVEVHANEFTFEESISTGEIERKVESPINKAQQFKSILQNRKNENNKKSF 534

Query: 524 -TIYVGDSIGDLLCLLEADVGIVIGSSSSLKRLATQFGVSFVPLYQQVVRKQKALTEETR 583
            ++Y+GDS+GDLLCLLEAD+GIV+ SSSSL+R+ + FGVSFVPL+  +V+KQK  TEE+ 
Sbjct: 535 LSVYIGDSVGDLLCLLEADIGIVVSSSSSLRRVGSHFGVSFVPLFSGIVQKQKQHTEESS 594

Query: 584 CS-WKGLPGVLYTVNTWAEIHAFVLG 605
            S WKGL G LYTV++WAEIH+F LG
Sbjct: 595 SSAWKGLSGTLYTVSSWAEIHSFALG 615

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880589.12.3e-27578.59bifunctional TH2 protein, mitochondrial [Benincasa hispida][more]
KAA0038327.11.1e-27477.27putative aminopyrimidine aminohydrolase [Cucumis melo var. makuwa] >TYJ97070.1 p... [more]
XP_008443738.13.3e-27477.11PREDICTED: probable aminopyrimidine aminohydrolase, mitochondrial isoform X1 [Cu... [more]
XP_023515856.11.3e-27378.00bifunctional TH2 protein, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
XP_022988051.12.8e-27377.83bifunctional TH2 protein, mitochondrial-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
F4KFT74.3e-19259.54Bifunctional TH2 protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=TH2 P... [more]
P446593.3e-1428.57Uncharacterized protein HI_0358 OS=Haemophilus influenzae (strain ATCC 51907 / D... [more]
Q8FTH86.8e-1226.27Thiamine biosynthesis multifunctional protein ThiED OS=Corynebacterium efficiens... [more]
Q082242.3e-0725.94Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20 OS=Saccharomyces ce... [more]
O942667.3e-0625.46Putative hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 2 OS=Schizosacch... [more]
Match NameE-valueIdentityDescription
A0A5A7T9M15.5e-27577.27Putative aminopyrimidine aminohydrolase OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3B8T01.6e-27477.11probable aminopyrimidine aminohydrolase, mitochondrial isoform X1 OS=Cucumis mel... [more]
A0A6J1JG451.4e-27377.83bifunctional TH2 protein, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A0A0LY722.3e-27376.82TENA_THI-4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G665890 ... [more]
A0A6J1HD462.6e-27277.50bifunctional TH2 protein, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G32470.13.1e-19359.54Haem oxygenase-like, multi-helical [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 164..420
e-value: 1.9E-13
score: 52.6
IPR004305Thiaminase-2/PQQCPFAMPF03070TENA_THI-4coord: 54..141
e-value: 4.5E-10
score: 39.7
IPR016084Haem oxygenase-like, multi-helicalGENE3D1.20.910.10coord: 2..144
e-value: 1.7E-28
score: 102.0
IPR016084Haem oxygenase-like, multi-helicalSUPERFAMILY48613Heme oxygenase-likecoord: 9..142
NoneNo IPR availablePANTHERPTHR43198:SF2SI:CH1073-67J19.1-RELATEDcoord: 3..466
NoneNo IPR availablePANTHERPTHR43198BIFUNCTIONAL TH2 PROTEINcoord: 3..466
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 161..422

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0005304.1Sed0005304.1mRNA
Sed0005304.2Sed0005304.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
biological_process GO:0009228 thiamine biosynthetic process
cellular_component GO:0005829 cytosol
cellular_component GO:0005739 mitochondrion
molecular_function GO:0042131 thiamine phosphate phosphatase activity