Sed0005287 (gene) Chayote v1

BLAST of Sed0005287 vs. NCBI nr
Match: KAG6588921.1 (E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 514.6 bits (1324), Expect = 7.3e-142
Identity = 302/388 (77.84%), Postives = 326/388 (84.02%), Query Frame = 0

Query: 1   MVQEESTASASEPELETVESKPLEIGSDADCFLSPRFKSAAAMAGWDEEALLMASLVVDD 60
           MVQE ST SASEPE ETV SKPLEIG+D   + SPRFKSAAAMAGWDEEALL+ASLVV+D
Sbjct: 1   MVQEGSTTSASEPERETVGSKPLEIGNDGAGYFSPRFKSAAAMAGWDEEALLIASLVVED 60

Query: 61  TPDREFRHKKRSVLQCRSPPSGSRRKRRTSSSLISIPVAVLDLEEEESTVKDKAPKQ-EE 120
           TPDREFR KKR VLQC+SP SGSRRKRR S++LISIP AVLDLEEEESTVKD APKQ +E
Sbjct: 61  TPDREFRQKKRLVLQCKSPLSGSRRKRRASANLISIPAAVLDLEEEESTVKDDAPKQDQE 120

Query: 121 KAEAEPKKSYPLVEQKAGSSSSSSSPCSTLPCMDKLREELSCAICLEICFEPSTTPCGHS 180
            AEA+PKKS  L EQKAG+SSSSS+ CSTLPCMDKLREELSCAICLEICFEPSTTPCGHS
Sbjct: 121 TAEADPKKSDSLDEQKAGASSSSST-CSTLPCMDKLREELSCAICLEICFEPSTTPCGHS 180

Query: 181 FCKKCLRSAAEKCGKKCPKCRQLISNGRSCTINTVLWNTIQLLFPKEVETRKEAKEGSSH 240
           FCKKCLRSAA+KCGKKCPKCRQLISNGRSCT+NTVLWNTIQLLFP+EVE RKEAK G S 
Sbjct: 181 FCKKCLRSAADKCGKKCPKCRQLISNGRSCTVNTVLWNTIQLLFPREVEARKEAKGGCSS 240

Query: 241 QKKKNQDPEKA--------NTRPTGTSSRHGSSSSSRRRGEITVRDEDGESISRMTRRAA 300
             KK QD EKA        N R T TSSRHGS+SS RR  EI  RDEDGES  RMT++A 
Sbjct: 241 HDKKIQDTEKAFYSSLSNHNMRATRTSSRHGSTSSRRR--EIMGRDEDGESNGRMTQQAP 300

Query: 301 LSND-ESRV-TRTHHRSIRPVRTITRDADVRSSSRRGTPDQDQDAALALRLQREEFMEAF 360
            SN   SRV +R HHRS+RPVR  TRDADV  S RRGTPDQDQ+AALALRL+REEFMEAF
Sbjct: 301 FSNAVSSRVQSRMHHRSVRPVRRTTRDADV--SRRRGTPDQDQEAALALRLRREEFMEAF 360

Query: 361 RDTPVQTRSSLSLARANLRAM-ARAAVN 377
           RD  VQTRSS+SLARANLRA+ AR A+N
Sbjct: 361 RDNQVQTRSSVSLARANLRALAARVAIN 383

BLAST of Sed0005287 vs. NCBI nr
Match: XP_022928318.1 (LON peptidase N-terminal domain and RING finger protein 3-like [Cucurbita moschata])

HSP 1 Score: 512.3 bits (1318), Expect = 3.6e-141
Identity = 299/382 (78.27%), Postives = 322/382 (84.29%), Query Frame = 0

Query: 1   MVQEESTASASEPELETVESKPLEIGSDADCFLSPRFKSAAAMAGWDEEALLMASLVVDD 60
           MVQE ST SASEPE ETV SKPLEIG+D   + SPRFKSAAAMAGWDEEALL+ASLVV+D
Sbjct: 1   MVQEGSTTSASEPERETVGSKPLEIGNDGAGYFSPRFKSAAAMAGWDEEALLIASLVVED 60

Query: 61  TPDREFRHKKRSVLQCRSPPSGSRRKRRTSSSLISIPVAVLDLEEEESTVKDKAPKQ-EE 120
           TPDREFR KKR VLQC+SP SGSRRKRR S++LISIP AVLDLEEEESTVKD APKQ +E
Sbjct: 61  TPDREFRQKKRLVLQCKSPLSGSRRKRRASANLISIPAAVLDLEEEESTVKDDAPKQDQE 120

Query: 121 KAEAEPKKSYPLVEQKAGSSSSSSSPCSTLPCMDKLREELSCAICLEICFEPSTTPCGHS 180
            AEA+PKKS  L EQKAG+SSSSS+ CSTLPCMDKLREELSCAICLEICFEPSTTPCGHS
Sbjct: 121 TAEADPKKSDSLDEQKAGASSSSST-CSTLPCMDKLREELSCAICLEICFEPSTTPCGHS 180

Query: 181 FCKKCLRSAAEKCGKKCPKCRQLISNGRSCTINTVLWNTIQLLFPKEVETRKEAKEGSSH 240
           FCKKCLRSAA+KCGKKCPKCRQLISNGRSCT+NTVLWNTIQLLFP+EVE RKEAK G S 
Sbjct: 181 FCKKCLRSAADKCGKKCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVEARKEAKGGCSS 240

Query: 241 QKKKNQDPEKA--------NTRPTGTSSRHGSSSSSRRRGEITVRDEDGESISRMTRRAA 300
             KK QD EKA        N R T TSSRHGS+SS RR  EI  RDEDGES  RM ++A 
Sbjct: 241 HDKKIQDTEKAFYSSSSNHNMRATRTSSRHGSTSSRRR--EIMGRDEDGESNGRMMQQAP 300

Query: 301 LSND-ESRV-TRTHHRSIRPVRTITRDADVRSSSRRGTPDQDQDAALALRLQREEFMEAF 360
            SN   SRV +R HHRS+RPVRT TRDADV  S RRGTPDQDQ+AALALRL+REEFMEAF
Sbjct: 301 FSNAVSSRVQSRMHHRSVRPVRTTTRDADV--SRRRGTPDQDQEAALALRLRREEFMEAF 360

Query: 361 RDTPVQTRSSLSLARANLRAMA 372
           RD  VQTRSS+SLARANLRA+A
Sbjct: 361 RDNQVQTRSSVSLARANLRALA 377

BLAST of Sed0005287 vs. NCBI nr
Match: XP_023531716.1 (E3 ubiquitin-protein ligase RNF168-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 509.2 bits (1310), Expect = 3.1e-140
Identity = 305/393 (77.61%), Postives = 327/393 (83.21%), Query Frame = 0

Query: 1   MVQEESTASASEPELETVESKPLEIGSDADCFLSPRFKSAAAMAGWDEEALLMASLVVDD 60
           MVQE ST SASEPE ET+ SKPLEIG+D + + SPRFKSAAAMAGWDEEALL+ASLVV+D
Sbjct: 1   MVQEGSTTSASEPERETIGSKPLEIGNDGESYFSPRFKSAAAMAGWDEEALLIASLVVED 60

Query: 61  TPDREFRHKKRSVLQCRSPPSGSRRKRRTSSSLISIPVAVLDLEEEESTVKDKAPKQE-E 120
           TPDREFR KKR VLQC+SP SGSRRKRR S++LISIP AVLDLEEEESTVKD APKQE E
Sbjct: 61  TPDREFRQKKRLVLQCKSPLSGSRRKRRDSANLISIPAAVLDLEEEESTVKDDAPKQEQE 120

Query: 121 KAEAEPKKSYPLVEQKAG--SSSSSSSPCSTLPCMDKLREELSCAICLEICFEPSTTPCG 180
            AEA+PKKS  L EQKAG  SSSSSSS CSTLPCMDKLREELSCAICLEICFEPSTTPCG
Sbjct: 121 TAEADPKKSDSLDEQKAGASSSSSSSSTCSTLPCMDKLREELSCAICLEICFEPSTTPCG 180

Query: 181 HSFCKKCLRSAAEKCGKKCPKCRQLISNGRSCTINTVLWNTIQLLFPKEVETRKEAKEGS 240
           HSFCKKCLRSAA+KCGKKCPKCRQLISNGRSCT+NTVLWNTIQLLFP+EVE RKEAK G 
Sbjct: 181 HSFCKKCLRSAADKCGKKCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVEARKEAKGGC 240

Query: 241 SHQKKKNQDPEKA---------NTRPTGTSSRHGSSSSSRRRGEITVRDEDGESISRMTR 300
           S   KK QD EKA         NTR T TSSRHGSS S RR  EIT RDED ES  RM +
Sbjct: 241 SSHDKKIQDTEKAIYNSSLQNHNTRATRTSSRHGSSISRRR--EITGRDEDDESNGRMMQ 300

Query: 301 RAALSND-ESRV-TRTHHRSIRPVRTITRDADVRSSSRRGTPDQDQDAALALRLQREEFM 360
           +A  SN   SRV +R HHRS+RPVRT TRDADV  S RRGTPDQDQ+AALALRL+REEFM
Sbjct: 301 QAPFSNAVSSRVQSRMHHRSVRPVRTTTRDADV--SRRRGTPDQDQEAALALRLRREEFM 360

Query: 361 EAFRD-TPVQTR-SSLSLARANLRAM-ARAAVN 377
           EAFRD   VQTR SS+SLARANLRA+ AR A+N
Sbjct: 361 EAFRDNNQVQTRSSSVSLARANLRALAARVAIN 389

BLAST of Sed0005287 vs. NCBI nr
Match: KAG7017808.1 (Tripartite motif-containing protein 65, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 506.5 bits (1303), Expect = 2.0e-139
Identity = 299/397 (75.31%), Postives = 330/397 (83.12%), Query Frame = 0

Query: 1   MVQEESTASASEPELETVESKPLEIGSDADCFLSPRFKSAAAMAGWDEEALLMASLVVDD 60
           MVQE STASASEPE   +ES+PLE G+DAD F SPRFKSAAAMAGWDEEALL+ASLVVDD
Sbjct: 1   MVQEGSTASASEPERRAIESRPLESGNDADSFFSPRFKSAAAMAGWDEEALLIASLVVDD 60

Query: 61  TPDREFRHKKRSVLQCRSPPSGSRRKRRTSSSLISIPVAVLDLEEEESTVKDKAPKQE-E 120
           TP+REF+ KKRSVL C+SP SGSRRKRRTS+S+ISIPVAVLDL+ EE TV+D A KQ+ E
Sbjct: 61  TPEREFQQKKRSVLHCKSPTSGSRRKRRTSTSIISIPVAVLDLDAEEPTVRDDATKQDPE 120

Query: 121 KAEAEPKKSYPLVEQKAGSSSSSSSPCSTLPCMDKLREELSCAICLEICFEPSTTPCGHS 180
            AEAEPKKS  L+EQKAG SS S   CSTLPC+DKLREELSCAICLEICFEPSTTPCGHS
Sbjct: 121 TAEAEPKKSNSLIEQKAGDSSLS---CSTLPCIDKLREELSCAICLEICFEPSTTPCGHS 180

Query: 181 FCKKCLRSAAEKCGKKCPKCRQLISNGRSCTINTVLWNTIQLLFPKEVETRKEAKEGSSH 240
           FCKKCLRSAA++CGK+CPKCRQLISNGRSCT+NTVLWNTIQLLFP+EVE RK   +  S 
Sbjct: 181 FCKKCLRSAADRCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVEARKVVAKECSS 240

Query: 241 QKKKNQDPEKA--------NTRPTGTSSRHGSSSSSRRRGEITVR-DEDGESISRMTRRA 300
            +KK QDPEKA        NT+ T TSSR G  SSSRRRGEITVR DEDGE  +R+ ++A
Sbjct: 241 PEKKVQDPEKAFYTSLQNHNTQATDTSSRRG--SSSRRRGEITVRDDEDGERDNRVMQQA 300

Query: 301 ALSNDESRV-TRTHHRSIRPVRTITRDADVRSSSRRGTPDQDQDAALALRLQREEFMEAF 360
           A SN ESRV +R   RSIR +R  TRD+DVR SSRRGTPDQDQDAALALRLQREEFMEAF
Sbjct: 301 ASSNAESRVPSRMQRRSIRSMRIATRDSDVR-SSRRGTPDQDQDAALALRLQREEFMEAF 360

Query: 361 RDTPVQT-RSSLSLARANLRAMARAAVNLRVNRHSDL 386
           RDTPVQT RSSLSLARANLRAMA  A    V+RH +L
Sbjct: 361 RDTPVQTRRSSLSLARANLRAMASRAA---VSRHRNL 388

BLAST of Sed0005287 vs. NCBI nr
Match: KAG6581080.1 (Tripartite motif-containing protein 65, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 504.2 bits (1297), Expect = 9.9e-139
Identity = 298/397 (75.06%), Postives = 329/397 (82.87%), Query Frame = 0

Query: 1   MVQEESTASASEPELETVESKPLEIGSDADCFLSPRFKSAAAMAGWDEEALLMASLVVDD 60
           MVQE STASASEPE   +ES+PLE G+DAD F SPRFKSAAAMAGWDEEALL+ASLVVDD
Sbjct: 1   MVQEGSTASASEPERRAIESRPLESGNDADSFFSPRFKSAAAMAGWDEEALLIASLVVDD 60

Query: 61  TPDREFRHKKRSVLQCRSPPSGSRRKRRTSSSLISIPVAVLDLEEEESTVKDKAPKQE-E 120
           TP+REF+ KKRSVL C+SP SGSRRKRRTS+S+ISIPVAVLDL+ EE TV+D A KQ+ E
Sbjct: 61  TPEREFQQKKRSVLHCKSPTSGSRRKRRTSTSIISIPVAVLDLDAEEPTVRDDATKQDPE 120

Query: 121 KAEAEPKKSYPLVEQKAGSSSSSSSPCSTLPCMDKLREELSCAICLEICFEPSTTPCGHS 180
            AEAEPKKS  L+EQKAG SS S   CSTLPC+DKLREELSCAICLEICFEPSTTPCGHS
Sbjct: 121 TAEAEPKKSNSLIEQKAGDSSLS---CSTLPCIDKLREELSCAICLEICFEPSTTPCGHS 180

Query: 181 FCKKCLRSAAEKCGKKCPKCRQLISNGRSCTINTVLWNTIQLLFPKEVETRKEAKEGSSH 240
           FCKKCLRSAA++CGK+CPKCRQLISNGRSCT+NTVLWNTIQLLFP+EVE RK   +  S 
Sbjct: 181 FCKKCLRSAADRCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVEARKVVAKECSS 240

Query: 241 QKKKNQDPEKA--------NTRPTGTSSRHGSSSSSRRRGEITVR-DEDGESISRMTRRA 300
            +KK QDPEKA        NT+ T TSSR G  SSSRRRGEITVR DEDGE  +R+ ++A
Sbjct: 241 PEKKVQDPEKAFYTSLQNHNTQATDTSSRRG--SSSRRRGEITVRDDEDGERDNRVMQQA 300

Query: 301 ALSNDESRV-TRTHHRSIRPVRTITRDADVRSSSRRGTPDQDQDAALALRLQREEFMEAF 360
           A SN ESRV +R   RSIR +R  TRD+DVR SSRRGTPDQDQDAALALRLQREEFMEAF
Sbjct: 301 ASSNAESRVPSRMQRRSIRSMRIATRDSDVR-SSRRGTPDQDQDAALALRLQREEFMEAF 360

Query: 361 RDTPVQ-TRSSLSLARANLRAMARAAVNLRVNRHSDL 386
           RDTPVQ  RSSLSLARANLRAMA  A    V+RH +L
Sbjct: 361 RDTPVQPRRSSLSLARANLRAMASRAA---VSRHRNL 388

BLAST of Sed0005287 vs. ExPASy Swiss-Prot
Match: Q0IIM1 (E3 ubiquitin-protein ligase RNF168 OS=Bos taurus OX=9913 GN=RNF168 PE=2 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 1.4e-07
Identity = 62/212 (29.25%), Postives = 93/212 (43.87%), Query Frame = 0

Query: 158 ELSCAICLEICFEPSTTPCGHSFCKKCLRSAAEKCGKKCPKCRQLISNG---RSCT---I 217
           E  C IC+EI FEP T PC H+ CK C  S  EK    CP CR+ +S+    RS T   +
Sbjct: 13  ECQCQICVEILFEPVTLPCNHTLCKPCFESTVEKASLCCPFCRRRVSSWARYRSRTNSLV 72

Query: 218 NTVLWNTIQLLFPKEVETRKEAKEGSSHQKKKNQDPEKANTRPTGTSSRHGSSSSSRRRG 277
           N  LW  IQ  +PKE + R   +E  S +   +  P +  ++P                G
Sbjct: 73  NMELWEIIQKHYPKECKLRASGQE--SKEIVDDYQPVRLLSKP----------------G 132

Query: 278 EITVRDEDGESISRM-TRRAALSNDESRVTRTHHRSI--RPVRTITRDADVRSSSRRGTP 337
           E+  R E  E IS++   R A   +E++ +  + + +         R A+ R        
Sbjct: 133 EL--RREYEEEISKVEAERRACEEEENKASEEYIQKLLAEEEEEEKRQAEKRHREMEEQL 192

Query: 338 DQDQDAALALRLQREEFME-AFRDTPVQTRSS 360
             D++ A  L L    F E +   +P+ +R S
Sbjct: 193 KSDEELARRLSLDINNFCEGSVLASPLNSRKS 204

BLAST of Sed0005287 vs. ExPASy Swiss-Prot
Match: Q92021 (Nuclear factor 7, brain OS=Xenopus laevis OX=8355 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.9e-07
Identity = 39/106 (36.79%), Postives = 59/106 (55.66%), Query Frame = 0

Query: 113 KAPKQEEKAEAEPKKSYPLVEQKAGSSSSSSSPCSTLPCMDKLREELSCAICLEICFEPS 172
           K P++ +  E EP+     VE+K  S ++SS     L       EEL+C +C+E+  +P 
Sbjct: 102 KTPQKRKIEEPEPEPKKAKVEEKDASKNASS-----LGAAGDFAEELTCPLCVELFKDPV 161

Query: 173 TTPCGHSFCKKCLRSAAE-KCGKKCPKCRQLISNGRSCTINTVLWN 218
              CGH+FC+ C+  A E +    CP+CR+ I++ R  TIN VL N
Sbjct: 162 MVACGHNFCRSCIDKAWEGQSSFACPECRESITD-RKYTINRVLAN 201

BLAST of Sed0005287 vs. ExPASy Swiss-Prot
Match: Q8IYW5 (E3 ubiquitin-protein ligase RNF168 OS=Homo sapiens OX=9606 GN=RNF168 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 5.5e-07
Identity = 55/209 (26.32%), Postives = 87/209 (41.63%), Query Frame = 0

Query: 158 ELSCAICLEICFEPSTTPCGHSFCKKCLRSAAEKCGKKCPKCRQLISN------GRSCTI 217
           E  C IC+EI  EP T PC H+ CK C +S  EK    CP CR+ +S+       R+  +
Sbjct: 13  ECQCGICMEILVEPVTLPCNHTLCKPCFQSTVEKASLCCPFCRRRVSSWTRYHTRRNSLV 72

Query: 218 NTVLWNTIQLLFPKEVETRKEAKEGSSHQKKKNQDPEKANTRPTGTSSRHGSSSSSRRRG 277
           N  LW  IQ  +P+E + R   +E  S +   +  P +  ++P G   R      S+   
Sbjct: 73  NVELWTIIQKHYPRECKLRASGQE--SEEVADDYQPVRLLSKP-GELRREYEEEISKVAA 132

Query: 278 EITVRDEDGESISRMTRRAALSNDESRVTRTHHRSIRPVRTITRDADVRSSSRRGTPDQD 337
           E    +E+    S    +  L+ +E                  R A+ R  +       D
Sbjct: 133 ERRASEEEENKASEEYIQRLLAEEEEEE--------------KRQAEKRRRAMEEQLKSD 192

Query: 338 QDAALALRLQREEFME-AFRDTPVQTRSS 360
           ++ A  L +    F E +   +P+ +R S
Sbjct: 193 EELARKLSIDINNFCEGSISASPLNSRKS 204

BLAST of Sed0005287 vs. ExPASy Swiss-Prot
Match: Q6PJ69 (Tripartite motif-containing protein 65 OS=Homo sapiens OX=9606 GN=TRIM65 PE=1 SV=3)

HSP 1 Score: 57.0 bits (136), Expect = 5.5e-07
Identity = 51/189 (26.98%), Postives = 79/189 (41.80%), Query Frame = 0

Query: 155 LREELSCAICLEICFEPSTTPCGHSFCKKCLRSAAEKCGKKCPKCRQLISNGRSCTINTV 214
           L E+L+CAICL +  +P T PCGH+FC  C+R   ++CGK CP+CR+   +G     N  
Sbjct: 6   LEEKLTCAICLGLYQDPVTLPCGHNFCGACIRDWWDRCGKACPECREPFPDGAELRRNVA 65

Query: 215 LWNTIQLLFPKEVETRKEAKEGSSHQKKKNQDPEKANTRPTGTSSRHGSSSSSRRRGEIT 274
           L   ++++           + G +     +  P      P     RHG      R  E+ 
Sbjct: 66  LSGVLEVV-----------RAGPARDPGPDPGP---GPDPAARCPRHG------RPLELF 125

Query: 275 VRDEDGESISRMTRRAALSNDESRVTRTHHRSIRPVRTITRDADVRSSSRRGTPDQDQDA 334
            R E          R   S    R  R H R++     + R+A +R+S         Q  
Sbjct: 126 CRTEG---------RCVCSVCTVRECRLHERALLDAERLKREAQLRASLEVTQQQATQAE 165

Query: 335 ALALRLQRE 344
              L L+++
Sbjct: 186 GQLLELRKQ 165

BLAST of Sed0005287 vs. ExPASy Swiss-Prot
Match: Q9D4H7 (LON peptidase N-terminal domain and RING finger protein 3 OS=Mus musculus OX=10090 GN=Lonrf3 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.6e-06
Identity = 43/136 (31.62%), Postives = 66/136 (48.53%), Query Frame = 0

Query: 105 EEESTVKDKAPKQEEKAE--AEPKKSYPLVEQKAGSSSSSSSPCSTLPCMDKLREELSCA 164
           +E++ + +K  KQ+  AE  A+P  S PL     GS  +S               +L C+
Sbjct: 423 QEDTELPNKVSKQDFPAEQGAKPDLSNPL-----GSFDAS---------------DLECS 482

Query: 165 ICLEICFEPSTTPCGHSFCKKCLRSAAEKCGKKCPKCRQLISN---GRSCTINTVLWNTI 224
           +C+ + +EP TTPCGH+FC KCL    +    KCP C+ ++      R  + N +L   I
Sbjct: 483 LCMRLFYEPVTTPCGHTFCLKCLERCLDH-NAKCPLCKDVLLQCLPSRKYSKNVILEELI 537

Query: 225 QLLFPKEVETRKEAKE 236
               P+E + RK   E
Sbjct: 543 ATFLPEEFKERKRLYE 537

BLAST of Sed0005287 vs. ExPASy TrEMBL
Match: A0A6J1ENN5 (LON peptidase N-terminal domain and RING finger protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111435179 PE=4 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.8e-141
Identity = 299/382 (78.27%), Postives = 322/382 (84.29%), Query Frame = 0

Query: 1   MVQEESTASASEPELETVESKPLEIGSDADCFLSPRFKSAAAMAGWDEEALLMASLVVDD 60
           MVQE ST SASEPE ETV SKPLEIG+D   + SPRFKSAAAMAGWDEEALL+ASLVV+D
Sbjct: 1   MVQEGSTTSASEPERETVGSKPLEIGNDGAGYFSPRFKSAAAMAGWDEEALLIASLVVED 60

Query: 61  TPDREFRHKKRSVLQCRSPPSGSRRKRRTSSSLISIPVAVLDLEEEESTVKDKAPKQ-EE 120
           TPDREFR KKR VLQC+SP SGSRRKRR S++LISIP AVLDLEEEESTVKD APKQ +E
Sbjct: 61  TPDREFRQKKRLVLQCKSPLSGSRRKRRASANLISIPAAVLDLEEEESTVKDDAPKQDQE 120

Query: 121 KAEAEPKKSYPLVEQKAGSSSSSSSPCSTLPCMDKLREELSCAICLEICFEPSTTPCGHS 180
            AEA+PKKS  L EQKAG+SSSSS+ CSTLPCMDKLREELSCAICLEICFEPSTTPCGHS
Sbjct: 121 TAEADPKKSDSLDEQKAGASSSSST-CSTLPCMDKLREELSCAICLEICFEPSTTPCGHS 180

Query: 181 FCKKCLRSAAEKCGKKCPKCRQLISNGRSCTINTVLWNTIQLLFPKEVETRKEAKEGSSH 240
           FCKKCLRSAA+KCGKKCPKCRQLISNGRSCT+NTVLWNTIQLLFP+EVE RKEAK G S 
Sbjct: 181 FCKKCLRSAADKCGKKCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVEARKEAKGGCSS 240

Query: 241 QKKKNQDPEKA--------NTRPTGTSSRHGSSSSSRRRGEITVRDEDGESISRMTRRAA 300
             KK QD EKA        N R T TSSRHGS+SS RR  EI  RDEDGES  RM ++A 
Sbjct: 241 HDKKIQDTEKAFYSSSSNHNMRATRTSSRHGSTSSRRR--EIMGRDEDGESNGRMMQQAP 300

Query: 301 LSND-ESRV-TRTHHRSIRPVRTITRDADVRSSSRRGTPDQDQDAALALRLQREEFMEAF 360
            SN   SRV +R HHRS+RPVRT TRDADV  S RRGTPDQDQ+AALALRL+REEFMEAF
Sbjct: 301 FSNAVSSRVQSRMHHRSVRPVRTTTRDADV--SRRRGTPDQDQEAALALRLRREEFMEAF 360

Query: 361 RDTPVQTRSSLSLARANLRAMA 372
           RD  VQTRSS+SLARANLRA+A
Sbjct: 361 RDNQVQTRSSVSLARANLRALA 377

BLAST of Sed0005287 vs. ExPASy TrEMBL
Match: A0A6J1F9B4 (E3 ubiquitin-protein ligase ORTHRUS 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441989 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 6.2e-139
Identity = 301/398 (75.63%), Postives = 331/398 (83.17%), Query Frame = 0

Query: 1   MVQEESTASASEPELETVESKPLEIGSDADCFLSPRFKSAAAMAGWDEEALLMASLVVDD 60
           MVQE S ASASEPE   +ES+PLE G+DAD F SPRFKSAAAMAGWDEEALL+ASLVVDD
Sbjct: 1   MVQEGSRASASEPERRAIESRPLESGNDADSFFSPRFKSAAAMAGWDEEALLIASLVVDD 60

Query: 61  TPDREFRHKKRSVLQCRSPPSGSRRKRRTSSSLISIPVAVLDLEEEESTVKDKAPKQE-E 120
           TP+REF+ KKRSVL C+SP SGSRRKRRTS+S+ISIPVAVLDL+ EE TV+D A KQ+ E
Sbjct: 61  TPEREFQQKKRSVLHCKSPTSGSRRKRRTSTSIISIPVAVLDLDAEEPTVRDDATKQDPE 120

Query: 121 KAEAEPKKSYPLVEQKAGSSSSSSSPCSTLPCMDKLREELSCAICLEICFEPSTTPCGHS 180
            AEAEPKKS  L+EQKAG SS S   CSTLPC+DKLREELSCAICLEICFEPSTTPCGHS
Sbjct: 121 TAEAEPKKSNSLIEQKAGDSSLS---CSTLPCIDKLREELSCAICLEICFEPSTTPCGHS 180

Query: 181 FCKKCLRSAAEKCGKKCPKCRQLISNGRSCTINTVLWNTIQLLFPKEVETRK-EAKEGSS 240
           FCKKCLRSAA++CGK+CPKCRQLISNGRSCT+NTVLWNTIQLLFP+EVE RK  AKE SS
Sbjct: 181 FCKKCLRSAADRCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPQEVEARKVVAKECSS 240

Query: 241 HQKKKNQDPEKA--------NTRPTGTSSRHGSSSSSRRRGEITVR-DEDGESISRMTRR 300
           H+KK  QDPE A        NT+ T TSSR G  SSSRRRGEITVR DEDGE  +R+ ++
Sbjct: 241 HEKKV-QDPENAFYSSLQNHNTQATDTSSRRG--SSSRRRGEITVRDDEDGERDNRVMQQ 300

Query: 301 AALSNDESRV-TRTHHRSIRPVRTITRDADVRSSSRRGTPDQDQDAALALRLQREEFMEA 360
           AA  N ESRV +R H +SIR +R  TRDADV  SSRRGTPDQDQDAALALRLQREEFMEA
Sbjct: 301 AASLNAESRVPSRMHRKSIRSMRMTTRDADV--SSRRGTPDQDQDAALALRLQREEFMEA 360

Query: 361 FRDTPVQT-RSSLSLARANLRAMARAAVNLRVNRHSDL 386
           FRDTPVQT RSSLSLARANLRAMA  A    V+RH +L
Sbjct: 361 FRDTPVQTRRSSLSLARANLRAMASRAA---VSRHQNL 387

BLAST of Sed0005287 vs. ExPASy TrEMBL
Match: A0A0A0LBY2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G592120 PE=4 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.7e-136
Identity = 297/397 (74.81%), Postives = 331/397 (83.38%), Query Frame = 0

Query: 1   MVQEESTASASEPELETVESKPLEIGSDADCFLSPRFKSAAAMAGWDEEALLMASLVVDD 60
           MV E STASAS       ESKPL I +DAD FLSPRFKSAAAMAGWDEEALL+ASLVVDD
Sbjct: 1   MVSEGSTASAS-------ESKPLGIANDADSFLSPRFKSAAAMAGWDEEALLIASLVVDD 60

Query: 61  TPDREFRHKKRSVLQCRSPPSGSRRKRRTSSSLISIPVAVLDLEEEESTVKDKAPKQEEK 120
           TP+REF+ KKRSVLQ +SP SGSRRKRRT +S+ISIPVAVLDL+E E TVKD  PKQE K
Sbjct: 61  TPEREFQQKKRSVLQRKSPASGSRRKRRTLTSIISIPVAVLDLDETEPTVKDDGPKQEPK 120

Query: 121 -AEAEPKKSYPLVEQKAGSSSSSSSPCSTLPCMDKLREELSCAICLEICFEPSTTPCGHS 180
            AEAE KKS  +VEQKA +SS +S   STLPCMDKLREELSCAICLEICFEPSTTPCGHS
Sbjct: 121 SAEAEAKKSDSMVEQKADASSLTS---STLPCMDKLREELSCAICLEICFEPSTTPCGHS 180

Query: 181 FCKKCLRSAAEKCGKKCPKCRQLISNGRSCTINTVLWNTIQLLFPKEVETRKEAKEGSSH 240
           FCKKCLRSAA+KCGK+CPKCRQLISNGRSCT+NTVLWNTIQLLFPKEVE RKEAKE +S 
Sbjct: 181 FCKKCLRSAADKCGKRCPKCRQLISNGRSCTVNTVLWNTIQLLFPKEVEARKEAKECNS- 240

Query: 241 QKKKNQDPEKA--------NTRPTGTSSRHGSSSSSRRRGEITVRDEDGESISRMTRRAA 300
           ++KK QDPEKA        +TRP GTSSRH   +S+RRRGEITV++E+GE  SR+T+RA 
Sbjct: 241 REKKIQDPEKAFYSSLQNYDTRPIGTSSRH---ASTRRRGEITVQEENGEWDSRVTQRAV 300

Query: 301 LSNDESRV-TRTHHRSIRPVRTITRDADVRSSSRRGTPDQDQDAALALRLQREEFMEAFR 360
            SN ESRV +R H RSIRP+R  TR  DVR SSRRGTPDQDQDAALALRLQREEF+EAFR
Sbjct: 301 SSNAESRVQSRMHRRSIRPMRMATRGVDVR-SSRRGTPDQDQDAALALRLQREEFLEAFR 360

Query: 361 D-TPVQTRSSLSLARANLRAMA-RAAVNLRVNRHSDL 386
           D T V TRSSLSL RAN+RA+A RAA+NLR++ H +L
Sbjct: 361 DTTQVHTRSSLSLTRANIRAIASRAAINLRISGHQNL 382

BLAST of Sed0005287 vs. ExPASy TrEMBL
Match: A0A5A7URU5 (LON peptidase N-terminal domain and RING finger protein 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold108G00490 PE=4 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 6.5e-136
Identity = 294/383 (76.76%), Postives = 320/383 (83.55%), Query Frame = 0

Query: 17  TVESKPLEIGSDADCFLSPRFKSAAAMAGWDEEALLMASLVVDDTPDREFRHKKRSVLQC 76
           T E KPL I +DAD FLSPRFKSAAAMAGWDEEALL+ASLVVDDTP+REF+ KKRS LQ 
Sbjct: 12  TSELKPLGIANDADSFLSPRFKSAAAMAGWDEEALLIASLVVDDTPEREFQQKKRSDLQR 71

Query: 77  RSP--PSGSRRKRRTSSSLISIPVAVLDLEEEESTVKDKAPKQEEK-AEAEPKKSYPLVE 136
           +SP   S SRRKRRT +S+ISIPV VLDLEEEE  VKD  PKQE K AEAE KK   +VE
Sbjct: 72  KSPASASASRRKRRTLTSIISIPVTVLDLEEEEPAVKDDGPKQEPKYAEAEAKKCDSMVE 131

Query: 137 QKAGSSSSSSSPCSTLPCMDKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAAEKCG 196
           QKA +SS SS   STLPCMDKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAA+KCG
Sbjct: 132 QKADASSLSS---STLPCMDKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAADKCG 191

Query: 197 KKCPKCRQLISNGRSCTINTVLWNTIQLLFPKEVETRKEAKEGSSHQKKKNQDPEKA--- 256
           K+CPKCRQLISNGRSCT+NTVLWNTIQLLFPKEVE RKE KE +SH+ KKNQDPEKA   
Sbjct: 192 KRCPKCRQLISNGRSCTVNTVLWNTIQLLFPKEVEARKEVKECNSHE-KKNQDPEKAFYS 251

Query: 257 -----NTRPTGTSSRHGSSSSSRRRGEITVRDEDGESISRMTRRAALSNDESRV-TRTHH 316
                NTRPTGTSSRH +S+SSRRR EITV +EDGE  SRMT+RAA SN ESRV +R H 
Sbjct: 252 SLQNYNTRPTGTSSRH-ASNSSRRRAEITVGEEDGEWDSRMTQRAASSNAESRVQSRMHR 311

Query: 317 RSIRPVRTITRDADVRSSSRRGTPDQDQDAALALRLQREEFMEAFRD-TPVQTRSSLSLA 376
           RSIRP+R  TR ADVR SSRRGTPDQDQDAALALRLQREEF+EAFRD T V TRSSLSL 
Sbjct: 312 RSIRPMRMATRGADVR-SSRRGTPDQDQDAALALRLQREEFLEAFRDTTQVHTRSSLSLT 371

Query: 377 RANLRAMA-RAAVNLRVNRHSDL 386
           RAN+RA+A RAA+NLR+  H +L
Sbjct: 372 RANIRAIASRAAINLRIGGHQNL 388

BLAST of Sed0005287 vs. ExPASy TrEMBL
Match: A0A1S3BPD3 (LON peptidase N-terminal domain and RING finger protein 3-like OS=Cucumis melo OX=3656 GN=LOC103491762 PE=4 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 2.5e-135
Identity = 293/383 (76.50%), Postives = 320/383 (83.55%), Query Frame = 0

Query: 17  TVESKPLEIGSDADCFLSPRFKSAAAMAGWDEEALLMASLVVDDTPDREFRHKKRSVLQC 76
           T E KPL I +DAD FLSPRFKSAAAMAGWDEEALL+ASLVVDDTP+REF+ KKRS LQ 
Sbjct: 12  TSELKPLGIANDADSFLSPRFKSAAAMAGWDEEALLIASLVVDDTPEREFQQKKRSDLQR 71

Query: 77  RSP--PSGSRRKRRTSSSLISIPVAVLDLEEEESTVKDKAPKQEEK-AEAEPKKSYPLVE 136
           +SP   S SRRKRRT +S+ISIPV VLDLEEEE  VKD  PKQE K AEAE KK   +VE
Sbjct: 72  KSPASASASRRKRRTLTSIISIPVTVLDLEEEEPAVKDDGPKQEPKYAEAEAKKCDSMVE 131

Query: 137 QKAGSSSSSSSPCSTLPCMDKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAAEKCG 196
           QKA +SS SS   STLPCMDKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAA+KCG
Sbjct: 132 QKADASSLSS---STLPCMDKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAADKCG 191

Query: 197 KKCPKCRQLISNGRSCTINTVLWNTIQLLFPKEVETRKEAKEGSSHQKKKNQDPEKA--- 256
           K+CPKCRQLISNGRSCT+NTVLWNTIQLLFPKEVE RKE KE +SH+ KKNQDPEKA   
Sbjct: 192 KRCPKCRQLISNGRSCTVNTVLWNTIQLLFPKEVEARKEVKECNSHE-KKNQDPEKAFYS 251

Query: 257 -----NTRPTGTSSRHGSSSSSRRRGEITVRDEDGESISRMTRRAALSNDESRV-TRTHH 316
                NTRPTGTSSRH +S+SSRRR EITV +EDGE  SRMT+RAA SN ESRV +R H 
Sbjct: 252 SLQNYNTRPTGTSSRH-ASNSSRRRAEITVGEEDGEWDSRMTQRAASSNAESRVQSRMHR 311

Query: 317 RSIRPVRTITRDADVRSSSRRGTPDQDQDAALALRLQREEFMEAFRD-TPVQTRSSLSLA 376
           RSIRP+R  TR ADVR SSRRGTPDQDQDAALALRLQREEF+EAFRD T V TRSSLSL 
Sbjct: 312 RSIRPMRMATRGADVR-SSRRGTPDQDQDAALALRLQREEFLEAFRDTTQVHTRSSLSLT 371

Query: 377 RANLRAMA-RAAVNLRVNRHSDL 386
           RAN+RA+A RAA+N R++ H +L
Sbjct: 372 RANIRAIASRAAINRRISGHQNL 388

BLAST of Sed0005287 vs. TAIR 10
Match: AT3G27330.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 260.0 bits (663), Expect = 3.0e-69
Identity = 180/360 (50.00%), Postives = 221/360 (61.39%), Query Frame = 0

Query: 26  GSDADCFLSPRFKSAAAMAGWDEEALLMASLVVDDTPDREF--RHKKRSVLQCRSPPSG- 85
           G +A+ F+SPRFKSAAAMAGWDEE L++AS VVDDTP+R    R ++ ++L   +PPSG 
Sbjct: 590 GENAEIFISPRFKSAAAMAGWDEEDLIIASFVVDDTPERSSSKRRRRSNLLFKSTPPSGS 649

Query: 86  SRRKRRTSSSLISIPVAVLD---LEEEESTVKDKAPKQEEKAEAEPKKSYPLVEQKAGSS 145
           SRR +R   SL+ +PV  LD     EEE + + K  K+E + E +        E+K    
Sbjct: 650 SRRNQRVKQSLVPLPVIDLDKVIRHEEEKSAEKKKKKREMETETKE-------EKKIDKD 709

Query: 146 SSSSSPCSTLPCMDKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAAEKCGKKCPKC 205
             S S    LPC+DKLR+ELSCAICLEICFEPSTT CGHSFCKKCLRSAA+KCG+KCPKC
Sbjct: 710 EKSLSDHVVLPCIDKLRDELSCAICLEICFEPSTTTCGHSFCKKCLRSAADKCGRKCPKC 769

Query: 206 RQLISNGRSCTINTVLWNTIQLLFPKEVETRKEAKEGSSHQKKKNQDPEKANTRPTGTSS 265
           RQLI NGR CT+NTVLWNTIQLLFPKEVE ++ A                AN +   T S
Sbjct: 770 RQLIGNGRYCTVNTVLWNTIQLLFPKEVEAQRAA---------------SANLKGKETPS 829

Query: 266 RHGSSSSSRRRGEITVRDEDGESISRMTRRAALSNDESRVTRTHHRSIRPVRTITRDADV 325
               +   R R   T   ++     R+ R      D SR+  +  RS R  R        
Sbjct: 830 PRNPNQRLRSRNRETALFQE-----RLQR-----EDISRLLVSEERSERSERR------R 889

Query: 326 RSSSRRGTPDQDQDAALALRLQREEFMEAFRDTPVQTRSS----LSLARANLRAMARAAV 376
           RS+S R   DQ++DAALA+RLQR+EF  AF  T  +T SS    LS ARANL+AMA  AV
Sbjct: 890 RSTSMR--LDQNRDAALAMRLQRQEFASAFGATTAETSSSSVSTLSRARANLQAMASRAV 909

BLAST of Sed0005287 vs. TAIR 10
Match: AT1G18660.4 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 74.7 bits (182), Expect = 1.8e-13
Identity = 34/89 (38.20%), Postives = 55/89 (61.80%), Query Frame = 0

Query: 152 MDKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAAEKCGKKCPKCRQLI-SNGRSCT 211
           M +  ++  C +CL++ +EP+TTPCGH+FC+ CL  + ++ G KCP CR +I    R+C 
Sbjct: 187 MAERSDDFDCTVCLKLLYEPATTPCGHTFCRSCLFQSMDR-GNKCPLCRTVIFMTPRTCA 246

Query: 212 INTVLWNTIQLLFPKEVETRKEAKEGSSH 240
           ++  L N I+  FP+E   RK  ++   H
Sbjct: 247 VSVTLNNIIEKNFPEEYAERKSEQDTLVH 274

BLAST of Sed0005287 vs. TAIR 10
Match: AT1G18660.2 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 74.7 bits (182), Expect = 1.8e-13
Identity = 34/89 (38.20%), Postives = 55/89 (61.80%), Query Frame = 0

Query: 152 MDKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAAEKCGKKCPKCRQLI-SNGRSCT 211
           M +  ++  C +CL++ +EP+TTPCGH+FC+ CL  + ++ G KCP CR +I    R+C 
Sbjct: 187 MAERSDDFDCTVCLKLLYEPATTPCGHTFCRSCLFQSMDR-GNKCPLCRTVIFMTPRTCA 246

Query: 212 INTVLWNTIQLLFPKEVETRKEAKEGSSH 240
           ++  L N I+  FP+E   RK  ++   H
Sbjct: 247 VSVTLNNIIEKNFPEEYAERKSEQDTLVH 274

BLAST of Sed0005287 vs. TAIR 10
Match: AT1G18660.3 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 74.7 bits (182), Expect = 1.8e-13
Identity = 34/89 (38.20%), Postives = 55/89 (61.80%), Query Frame = 0

Query: 152 MDKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAAEKCGKKCPKCRQLI-SNGRSCT 211
           M +  ++  C +CL++ +EP+TTPCGH+FC+ CL  + ++ G KCP CR +I    R+C 
Sbjct: 187 MAERSDDFDCTVCLKLLYEPATTPCGHTFCRSCLFQSMDR-GNKCPLCRTVIFMTPRTCA 246

Query: 212 INTVLWNTIQLLFPKEVETRKEAKEGSSH 240
           ++  L N I+  FP+E   RK  ++   H
Sbjct: 247 VSVTLNNIIEKNFPEEYAERKSEQDTLVH 274

BLAST of Sed0005287 vs. TAIR 10
Match: AT1G18660.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 74.7 bits (182), Expect = 1.8e-13
Identity = 34/89 (38.20%), Postives = 55/89 (61.80%), Query Frame = 0

Query: 152 MDKLREELSCAICLEICFEPSTTPCGHSFCKKCLRSAAEKCGKKCPKCRQLI-SNGRSCT 211
           M +  ++  C +CL++ +EP+TTPCGH+FC+ CL  + ++ G KCP CR +I    R+C 
Sbjct: 187 MAERSDDFDCTVCLKLLYEPATTPCGHTFCRSCLFQSMDR-GNKCPLCRTVIFMTPRTCA 246

Query: 212 INTVLWNTIQLLFPKEVETRKEAKEGSSH 240
           ++  L N I+  FP+E   RK  ++   H
Sbjct: 247 VSVTLNNIIEKNFPEEYAERKSEQDTLVH 274