Sed0005278 (gene) Chayote v1

Overview


Name	Sed0005278
Type	gene
Organism	Sechium edule (Chayote v1)
Description	protein NEN4-like
Location	LG10: 6537177 .. 6539202 (-)
RNA-Seq Expression	Sed0005278
Synteny	Sed0005278

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAACTAACAAAAACTTGAGCCCACAACTATTTTCCACAATACCAATGGAGGCTTCAAGTTCATTCAAGGAAGGTGCAGCTGAGATTGTCTTCTTCGACCTCGAAACCACGGTGCCAAATAGAGTTGGGCAGCGGTTTCGAGTATTGGAATTCGGTGCCATCGTTGTCTGCCCCTTGAAACTTGTGGAGCTTGAGAGCTTTACCACCCTCATCAGGCCAAAGGATTTATCGGTTGTGGCAGAGAGGTCTGGTCGACGTGAGGGGATAACGAGAGAAATCGTTGCAACTGCGCCATTGTTTGAGGAAGTTGCAGACAAGATATTCAGCATCTTGAATGGAAGGATATGGGCAGGTCATAACATTCAACGATTCGACTGCGTTCGTATTAGGGAGGCGTTCGCGGAGATTGGAAGGCCTGCTCCTAAGCCAGTTGGGATGGTTGATTCTTTAGGAGTCTTGACTCAGAAGTTTGGCAAGAGAGCTGGAAACATGAAGGTAATTAGATAACAACTTCAGCATTTTGCAAAATCCAAAGCCTGTAGATACATAACAATACATTAAGAATAGTTTTTGTCTCATATTTTGAGCTGTTTCATGTTTAGATGGCATCAATGGCTTCTTACTTTCAGCTGGGGCAGCAAAAACACAGGTATATAACAGATTAACTCCCTCTCCCTATGTCAGAGGCATACATGAATTAAGTTGGGATAAGAGAAATTTTTTGATTAATCCAACACCAAACCGTCTAGTCCTATATGTTAGAGCAACCCAACTTATATAAGTTGGACTATCAACTTTTTTTTTTAATTCGAATATATATATATATATATATATATATATATATATATATATTATTTATTTTTAATTTTTATATAAATCAAAATAATAATTTGTTCGATAGAATTGGTTAGACAGGATAGAGACTTTAGAATCGAGACTTTTTTAGACTCAATTCAACTATTTTTTTAATTAATAATAAAATTAAAAGAAATAAATGCATATATAATTACATTATAGGTGAAAAAAATATATTTTTTTAAATAATAATAAATTTGGATTAGACAAAAATTAATTTTAGGTTAACCCATAAATCAACCTTATTCATAATTTTTATTTTTTAAAATTTTAACTAAATCAAAATAAGTTGATAAAATTGAGTTGAGTTGATCTAATTTTTTGAATTGTCAATATTTTTAACATCCCTACCAAGCTCTATGTATGTTATACATACATCTCCTATGTTCACAAAAGTGCTTTGTGCAGGAGTCTTGATGATGTTCGTTTGAACTTGGAGGTCCTTAAGCATTGTGCTACCGTACTATTTCTGGTATGCGCGCAAAACGTTGGAGTTCAGCTGCTTATGCAGCTCGTTTGCACGCCTGAGTAATTGAAACGCAATTCCTCTTTGTCACTCATTTCTTATTTTGGTAATTTCTTTACTAGGAATCAACCCTCCCTAGCATATTGTCCGGGAAGTGGAAGACCTCATCCACCATAACAACTCGAAACCGAGCCAACGGGAGGCTGCATTGTAGGGAGGAAAGTAGCCGAAAATCTCCTCCGTCGTCGCCAGGATATCAACTCAGATCAGTTCCTTATTCAAGGGAAAGATTAGGAATTGCAAAGGTTCGTGGTTAAGGGTCTTTCTTTGAATGTCAATGATGCTAGCCTTCATATATTGATACTGAAAATGTGTTGATTTGAAACAGATGACAGCAAAGGTAAAGAATGTACTCTGCAGAGCTCAGGGGAGACAGCATTTCGATGTCTTACTCAAACATTCTCGATCGCTACTACGGTGACTCGAAACGGCCGAATTGACATTGTTTTGTGAGAGGGTGGAAGTTGGTTCATAGCATTGAAGAACATAAACAAACATGTATATAAGAGGAAATGATTATTTGAATGAAAGATTTACAACCTGTTTAGCATGGACAGAAAATGTTAAGAATTATATATCATCTTCTTGATGCTGTCATTTGGAGAGATATTCTTTGATGTAGAGATGGGAAAAATATAAGCTAC

mRNA sequence

CAAAACTAACAAAAACTTGAGCCCACAACTATTTTCCACAATACCAATGGAGGCTTCAAGTTCATTCAAGGAAGGTGCAGCTGAGATTGTCTTCTTCGACCTCGAAACCACGGTGCCAAATAGAGTTGGGCAGCGGTTTCGAGTATTGGAATTCGGTGCCATCGTTGTCTGCCCCTTGAAACTTGTGGAGCTTGAGAGCTTTACCACCCTCATCAGGCCAAAGGATTTATCGGTTGTGGCAGAGAGGTCTGGTCGACGTGAGGGGATAACGAGAGAAATCGTTGCAACTGCGCCATTGTTTGAGGAAGTTGCAGACAAGATATTCAGCATCTTGAATGGAAGGATATGGGCAGGTCATAACATTCAACGATTCGACTGCGTTCGTATTAGGGAGGCGTTCGCGGAGATTGGAAGGCCTGCTCCTAAGCCAGTTGGGATGGTTGATTCTTTAGGAGTCTTGACTCAGAAGTTTGGCAAGAGAGCTGGAAACATGAAGATGGCATCAATGGCTTCTTACTTTCAGCTGGGGCAGCAAAAACACAGGAGTCTTGATGATGTTCGTTTGAACTTGGAGGTCCTTAAGCATTGTGCTACCGTACTATTTCTGGAATCAACCCTCCCTAGCATATTGTCCGGGAAGTGGAAGACCTCATCCACCATAACAACTCGAAACCGAGCCAACGGGAGGCTGCATTGTAGGGAGGAAAGTAGCCGAAAATCTCCTCCGTCGTCGCCAGGATATCAACTCAGATCAGTTCCTTATTCAAGGGAAAGATTAGGAATTGCAAAGATGACAGCAAAGGTAAAGAATGTACTCTGCAGAGCTCAGGGGAGACAGCATTTCGATGTCTTACTCAAACATTCTCGATCGCTACTACGGTGACTCGAAACGGCCGAATTGACATTGTTTTGTGAGAGGGTGGAAGTTGGTTCATAGCATTGAAGAACATAAACAAACATGTATATAAGAGGAAATGATTATTTGAATGAAAGATTTACAACCTGTTTAGCATGGACAGAAAATGTTAAGAATTATATATCATCTTCTTGATGCTGTCATTTGGAGAGATATTCTTTGATGTAGAGATGGGAAAAATATAAGCTAC

Coding sequence (CDS)

ATGGAGGCTTCAAGTTCATTCAAGGAAGGTGCAGCTGAGATTGTCTTCTTCGACCTCGAAACCACGGTGCCAAATAGAGTTGGGCAGCGGTTTCGAGTATTGGAATTCGGTGCCATCGTTGTCTGCCCCTTGAAACTTGTGGAGCTTGAGAGCTTTACCACCCTCATCAGGCCAAAGGATTTATCGGTTGTGGCAGAGAGGTCTGGTCGACGTGAGGGGATAACGAGAGAAATCGTTGCAACTGCGCCATTGTTTGAGGAAGTTGCAGACAAGATATTCAGCATCTTGAATGGAAGGATATGGGCAGGTCATAACATTCAACGATTCGACTGCGTTCGTATTAGGGAGGCGTTCGCGGAGATTGGAAGGCCTGCTCCTAAGCCAGTTGGGATGGTTGATTCTTTAGGAGTCTTGACTCAGAAGTTTGGCAAGAGAGCTGGAAACATGAAGATGGCATCAATGGCTTCTTACTTTCAGCTGGGGCAGCAAAAACACAGGAGTCTTGATGATGTTCGTTTGAACTTGGAGGTCCTTAAGCATTGTGCTACCGTACTATTTCTGGAATCAACCCTCCCTAGCATATTGTCCGGGAAGTGGAAGACCTCATCCACCATAACAACTCGAAACCGAGCCAACGGGAGGCTGCATTGTAGGGAGGAAAGTAGCCGAAAATCTCCTCCGTCGTCGCCAGGATATCAACTCAGATCAGTTCCTTATTCAAGGGAAAGATTAGGAATTGCAAAGATGACAGCAAAGGTAAAGAATGTACTCTGCAGAGCTCAGGGGAGACAGCATTTCGATGTCTTACTCAAACATTCTCGATCGCTACTACGGTGA

Protein sequence

MEASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKDLSVVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKHCATVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSPGYQLRSVPYSRERLGIAKMTAKVKNVLCRAQGRQHFDVLLKHSRSLLR

Homology

[Close]

BLAST of Sed0005278 vs. NCBI nr
Match: XP_022133010.1 (protein NEN4 isoform X2 [Momordica charantia])

HSP 1 Score: 505.4 bits (1300), Expect = 3.2e-139
Identity = 254/278 (91.37%), Postives = 265/278 (95.32%), Query Frame = 0


Query: 1   MEASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKD 60
           MEASSS K+GAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRP D
Sbjct: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 61  LSVVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LS V  RS RR+GITRE VATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKH 180
           IGRPAP PVGMVDSLGVLT KFGKRAGNMKMAS+ASYFQLGQQKHRSLDDVR+NLEVLKH
Sbjct: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSPGYQLRSVPYS 240
           CATVLFLESTLPSIL GKWKTSST TTRNRANG+LHCREESSRKSPP+SPGYQLRSVPY+
Sbjct: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240

Query: 241 RERLGIAKMTAKVKNVLCRAQGRQHFDVLLKHSRSLLR 279
           RERLGI KMT KVKNVLCRAQG+Q+FD+LL+HSRSLLR
Sbjct: 241 RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 278

[Close]

BLAST of Sed0005278 vs. NCBI nr
Match: XP_023518830.1 (protein NEN4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 504.2 bits (1297), Expect = 7.1e-139
Identity = 258/278 (92.81%), Postives = 268/278 (96.40%), Query Frame = 0


Query: 1   MEASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKD 60
           MEASSSFKEG AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRP D
Sbjct: 1   MEASSSFKEG-AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 61  LSVVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LS VA RS RR+GITREIVATAPLFEEV+DKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVAARSSRRDGITREIVATAPLFEEVSDKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKH 180
           IGRPAP PVGMVDSLGVLTQKFGKRAGNMKMAS+ASYFQLGQQKHRSLDDVR+NLEVLKH
Sbjct: 121 IGRPAPMPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSPGYQLRSVPY- 240
           CATVLFLESTLPSIL GKWKTSSTITTRNRANG+LHCREE SRKSPPSSPGYQLRSVPY 
Sbjct: 181 CATVLFLESTLPSILQGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQLRSVPYA 240

Query: 241 SRERLGIAKMTAKVKNVLCRAQGRQHFDVLLKHSRSLL 278
           +RERLGIAKMTAKVKNV+CRAQG+Q F++LLKHSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCRAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of Sed0005278 vs. NCBI nr
Match: XP_022962945.1 (protein NEN4-like [Cucurbita moschata])

HSP 1 Score: 504.2 bits (1297), Expect = 7.1e-139
Identity = 258/278 (92.81%), Postives = 268/278 (96.40%), Query Frame = 0


Query: 1   MEASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKD 60
           MEASSSFKEG AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVEL+SFTTLIRP D
Sbjct: 1   MEASSSFKEG-AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELDSFTTLIRPTD 60

Query: 61  LSVVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LS VA RS RR+GITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVAARSSRRDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKH 180
           IGRPAP PVGMVDSLGVLTQKFGKRAGNMKMAS+ASYFQLGQQKHRSLDDVR+NLEVLKH
Sbjct: 121 IGRPAPMPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSPGYQLRSVPY- 240
           CATVLFLESTLPSIL GKWKTSSTITTRNRANG+LHCREE SRKSPPSSPGYQLRSVPY 
Sbjct: 181 CATVLFLESTLPSILQGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQLRSVPYA 240

Query: 241 SRERLGIAKMTAKVKNVLCRAQGRQHFDVLLKHSRSLL 278
           +RERLGIAKMTAKVKNV+CRAQG+Q F++LLKHSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCRAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of Sed0005278 vs. NCBI nr
Match: KAG7026830.1 (Protein NEN4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 502.7 bits (1293), Expect = 2.1e-138
Identity = 257/278 (92.45%), Postives = 267/278 (96.04%), Query Frame = 0


Query: 1   MEASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKD 60
           MEASSSFKEG AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVEL+SFTTLIRP D
Sbjct: 1   MEASSSFKEG-AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELDSFTTLIRPTD 60

Query: 61  LSVVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LS VA RS RR+GITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAF E
Sbjct: 61  LSAVAARSSRRDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFVE 120

Query: 121 IGRPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKH 180
           IGRPAP PVGMVDSLGVLTQKFGKRAGNMKMAS+ASYFQLGQQKHRSLDDVR+NLEVLKH
Sbjct: 121 IGRPAPMPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSPGYQLRSVPY- 240
           CATVLFLESTLPSIL GKWKTSSTITTRNRANG+LHCREE SRKSPPSSPGYQLRSVPY 
Sbjct: 181 CATVLFLESTLPSILEGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQLRSVPYA 240

Query: 241 SRERLGIAKMTAKVKNVLCRAQGRQHFDVLLKHSRSLL 278
           +RERLGIAKMTAKVKNV+CRAQG+Q F++LLKHSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCRAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of Sed0005278 vs. NCBI nr
Match: KAG6594865.1 (Protein NEN4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 501.5 bits (1290), Expect = 4.6e-138
Identity = 256/278 (92.09%), Postives = 267/278 (96.04%), Query Frame = 0


Query: 1   MEASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKD 60
           MEASSSF+EG AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVEL+SFTTLIRP D
Sbjct: 1   MEASSSFREG-AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELDSFTTLIRPTD 60

Query: 61  LSVVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LS VA RS RR+GITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAF E
Sbjct: 61  LSAVAARSSRRDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFVE 120

Query: 121 IGRPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKH 180
           IGRPAP PVGMVDSLGVLTQKFGKRAGNMKMAS+ASYFQLGQQKHRSLDDVR+NLEVLKH
Sbjct: 121 IGRPAPMPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSPGYQLRSVPY- 240
           CATVLFLESTLPSIL GKWKTSSTITTRNRANG+LHCREE SRKSPPSSPGYQLRSVPY 
Sbjct: 181 CATVLFLESTLPSILEGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQLRSVPYA 240

Query: 241 SRERLGIAKMTAKVKNVLCRAQGRQHFDVLLKHSRSLL 278
           +RERLGIAKMTAKVKNV+CRAQG+Q F++LLKHSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCRAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of Sed0005278 vs. ExPASy Swiss-Prot
Match: F4JJ23 (Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 4.4e-91
Identity = 176/268 (65.67%), Postives = 205/268 (76.49%), Query Frame = 0


Query: 13  EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKDLSVVAERSGRRE 72
           EIVFFDLET VPN+ GQ F +LEFGAI+VCP KL ELESFTTLI+PKDLSVV+ RS R +
Sbjct: 9   EIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRSD 68

Query: 73  GITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPKPVGMV 132
           GITR  V  AP FE+VA+KI  +LNGRIWAGHNI+RFDCVRI+EAFAEIG+ AP+P G++
Sbjct: 69  GITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGII 128

Query: 133 DSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKHCATVLFLESTLP 192
           DSLG+L+ KFGKRAGNMKMAS+A+YF LG QKHRSLDDVR+NLEVLKHCATVLFLESTLP
Sbjct: 129 DSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTLP 188

Query: 193 SILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSPGYQL--RSVPYSRERLGIAKMT 252
           + L GKW TSS I TR+R N                   YQ+  R++PYS+  L   KMT
Sbjct: 189 NHLEGKWHTSSKIMTRSRRN-------------------YQIAQRAMPYSKGSL--EKMT 248

Query: 253 AKVKNVLCRAQGRQHFDVLLKHSRSLLR 279
             VKN+L +AQG Q    L+ HS SLLR
Sbjct: 249 QNVKNLLSKAQGNQTLQSLINHSHSLLR 255

[Close]

BLAST of Sed0005278 vs. ExPASy Swiss-Prot
Match: Q9CA74 (Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 1.8e-68
Identity = 135/233 (57.94%), Postives = 172/233 (73.82%), Query Frame = 0


Query: 3   ASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKDLS 62
           ASS   +  +EI FFDLET VP + G+ F +LEFGAI+VCP +L EL S++TL+RP DLS
Sbjct: 2   ASSLGGDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLS 61

Query: 63  VVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIG 122
           +++  + RR GITR+ V +A  F E+ADK++ IL+GRIWAGHNI RFDCVRIREAFAEIG
Sbjct: 62  LISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIG 121

Query: 123 RPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKHCA 182
              P+P   +DSL +L+QKFGKRAG+MKMAS+A+YF LG Q HRSLDDVR+NLEV+K+CA
Sbjct: 122 LSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCA 181

Query: 183 TVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCR-----EESSRKSPPSSP 231
           TVLFLES++P IL+     S   + R R+NG+L          SS  SP + P
Sbjct: 182 TVLFLESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDP 234

[Close]

BLAST of Sed0005278 vs. ExPASy Swiss-Prot
Match: Q9FLR0 (Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.3e-68
Identity = 129/202 (63.86%), Postives = 159/202 (78.71%), Query Frame = 0


Query: 9   EGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKDLSVVAERS 68
           E  +EI FFD+ETTVP R GQRF +LEFG+I+VCP KL EL S+TTL++P DLS+++  S
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 69  GRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPKP 128
            R  GI R+ V  APLF ++AD ++ IL+GRIWAGHNI RFDC RIREAFAEIGR  P+P
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 129 VGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKHCATVLFLE 188
            G +DSLG+LTQKFG+RAG+MKMA++A YF LG Q HRSLDDVR+NLEVLK+CATVLFLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 189 STLPSILSGKWKTSSTITTRNR 211
           S+LP        +  TI++R R
Sbjct: 184 SSLPYAHVDNSVSPETISSRRR 204

[Close]

BLAST of Sed0005278 vs. ExPASy Swiss-Prot
Match: Q0V842 (Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.1e-65
Identity = 126/222 (56.76%), Postives = 167/222 (75.23%), Query Frame = 0


Query: 9   EGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKDLSVVAERS 68
           E  +EI FFD+ETT+P RVGQ + +LEFG+I+VCP KLVEL++++ L+RP +L+++  RS
Sbjct: 11  EDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRS 70

Query: 69  GRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPKP 128
            +  GI RE V +A  F ++AD ++ IL+GRIWAGHNI +FDC RIREAFAEIGR  P+P
Sbjct: 71  VKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEP 130

Query: 129 VGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKHCATVLFLE 188
            G +DSL +LTQ+FG+RAG+MKMA++A+YF LG Q HRSLDDVR+N EVLK+CATVLFLE
Sbjct: 131 KGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLE 190

Query: 189 STLPSILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSP 231
           S+LP  L     T++T  T +R       R  + +KSP  SP
Sbjct: 191 SSLPDELIENSVTTTTPETSSR-------RRRTIKKSPLQSP 225

[Close]

BLAST of Sed0005278 vs. ExPASy TrEMBL
Match: A0A6J1BU43 (protein NEN4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005707 PE=4 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.5e-139
Identity = 254/278 (91.37%), Postives = 265/278 (95.32%), Query Frame = 0


Query: 1   MEASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKD 60
           MEASSS K+GAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRP D
Sbjct: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 61  LSVVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LS V  RS RR+GITRE VATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKH 180
           IGRPAP PVGMVDSLGVLT KFGKRAGNMKMAS+ASYFQLGQQKHRSLDDVR+NLEVLKH
Sbjct: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSPGYQLRSVPYS 240
           CATVLFLESTLPSIL GKWKTSST TTRNRANG+LHCREESSRKSPP+SPGYQLRSVPY+
Sbjct: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240

Query: 241 RERLGIAKMTAKVKNVLCRAQGRQHFDVLLKHSRSLLR 279
           RERLGI KMT KVKNVLCRAQG+Q+FD+LL+HSRSLLR
Sbjct: 241 RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 278

[Close]

BLAST of Sed0005278 vs. ExPASy TrEMBL
Match: A0A6J1HGK3 (protein NEN4-like OS=Cucurbita moschata OX=3662 GN=LOC111463299 PE=4 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.4e-139
Identity = 258/278 (92.81%), Postives = 268/278 (96.40%), Query Frame = 0


Query: 1   MEASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKD 60
           MEASSSFKEG AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVEL+SFTTLIRP D
Sbjct: 1   MEASSSFKEG-AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELDSFTTLIRPTD 60

Query: 61  LSVVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LS VA RS RR+GITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVAARSSRRDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKH 180
           IGRPAP PVGMVDSLGVLTQKFGKRAGNMKMAS+ASYFQLGQQKHRSLDDVR+NLEVLKH
Sbjct: 121 IGRPAPMPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSPGYQLRSVPY- 240
           CATVLFLESTLPSIL GKWKTSSTITTRNRANG+LHCREE SRKSPPSSPGYQLRSVPY 
Sbjct: 181 CATVLFLESTLPSILQGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQLRSVPYA 240

Query: 241 SRERLGIAKMTAKVKNVLCRAQGRQHFDVLLKHSRSLL 278
           +RERLGIAKMTAKVKNV+CRAQG+Q F++LLKHSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCRAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of Sed0005278 vs. ExPASy TrEMBL
Match: A0A6J1BUQ8 (protein NEN4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005707 PE=4 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 8.5e-138
Identity = 254/282 (90.07%), Postives = 265/282 (93.97%), Query Frame = 0


Query: 1   MEASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKD 60
           MEASSS K+GAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRP D
Sbjct: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 61  LSVVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LS V  RS RR+GITRE VATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKH 180
           IGRPAP PVGMVDSLGVLT KFGKRAGNMKMAS+ASYFQLGQQKHRSLDDVR+NLEVLKH
Sbjct: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSPGYQLRSVPYS 240
           CATVLFLESTLPSIL GKWKTSST TTRNRANG+LHCREESSRKSPP+SPGYQLRSVPY+
Sbjct: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240

Query: 241 RERLGIAK----MTAKVKNVLCRAQGRQHFDVLLKHSRSLLR 279
           RERLGI K    MT KVKNVLCRAQG+Q+FD+LL+HSRSLLR
Sbjct: 241 RERLGIGKVQLQMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 282

[Close]

BLAST of Sed0005278 vs. ExPASy TrEMBL
Match: A0A6J1KYA7 (protein NEN4 OS=Cucurbita maxima OX=3661 GN=LOC111497460 PE=4 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.9e-137
Identity = 255/278 (91.73%), Postives = 265/278 (95.32%), Query Frame = 0


Query: 1   MEASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKD 60
           MEASSSFKEG AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRP D
Sbjct: 1   MEASSSFKEG-AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 61  LSVVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LS VA RS R +GITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVAARSSRHDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKH 180
           IG+PAP PVGMVDSLGVLTQKFGKRAGNMKMAS+ASYFQLGQQKHRSLDDVR+NLEVLKH
Sbjct: 121 IGKPAPNPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSPGYQLRSVPY- 240
           CATVLFLESTLPSIL GKWKTSSTITTRNRANG+LHCREE SRKSPPSSPGYQ RSVPY 
Sbjct: 181 CATVLFLESTLPSILQGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQARSVPYA 240

Query: 241 SRERLGIAKMTAKVKNVLCRAQGRQHFDVLLKHSRSLL 278
           +RERLGIAKMTAKVKNV+C AQG+Q F++LLKHSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCSAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of Sed0005278 vs. ExPASy TrEMBL
Match: A0A5D3CL58 (Protein NEN4 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002650 PE=4 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 4.4e-134
Identity = 247/278 (88.85%), Postives = 258/278 (92.81%), Query Frame = 0


Query: 1   MEASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKD 60
           MEAS SFKEGA EIVFFDLETTVP RVGQRFRVLEFGAIVVCP+KLVELESF TLI+P D
Sbjct: 5   MEASCSFKEGATEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFATLIKPTD 64

Query: 61  LSVVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LS VA RS R +GITRE VATAPLFE+VADKIFSILNGRIWAGHNI+RFDCVRI+EAFAE
Sbjct: 65  LSAVALRSSRPDGITREAVATAPLFEDVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAE 124

Query: 121 IGRPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKH 180
           IGRPAP PVGMVDSLGVLT KFGKRAGNMKMAS+ASYFQLGQQKHRSLDDVR+NLEVLKH
Sbjct: 125 IGRPAPTPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 184

Query: 181 CATVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSPGYQLRSVPYS 240
           CATVLFLESTLPSIL  KWKTS T TTRNRANG+LHCREESSRKSPPSSPGYQLRSVPYS
Sbjct: 185 CATVLFLESTLPSILQDKWKTSPTKTTRNRANGKLHCREESSRKSPPSSPGYQLRSVPYS 244

Query: 241 RERLGIAKMTAKVKNVLCRAQGRQHFDVLLKHSRSLLR 279
           RERLGI KMTAKVKNVL  AQG+QH +VLLKHSRSLLR
Sbjct: 245 RERLGIGKMTAKVKNVLYMAQGKQHLNVLLKHSRSLLR 282

[Close]

BLAST of Sed0005278 vs. TAIR 10
Match: AT4G39810.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 335.9 bits (860), Expect = 3.1e-92
Identity = 176/268 (65.67%), Postives = 205/268 (76.49%), Query Frame = 0


Query: 13  EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKDLSVVAERSGRRE 72
           EIVFFDLET VPN+ GQ F +LEFGAI+VCP KL ELESFTTLI+PKDLSVV+ RS R +
Sbjct: 9   EIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRSD 68

Query: 73  GITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPKPVGMV 132
           GITR  V  AP FE+VA+KI  +LNGRIWAGHNI+RFDCVRI+EAFAEIG+ AP+P G++
Sbjct: 69  GITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGII 128

Query: 133 DSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKHCATVLFLESTLP 192
           DSLG+L+ KFGKRAGNMKMAS+A+YF LG QKHRSLDDVR+NLEVLKHCATVLFLESTLP
Sbjct: 129 DSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTLP 188

Query: 193 SILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSPGYQL--RSVPYSRERLGIAKMT 252
           + L GKW TSS I TR+R N                   YQ+  R++PYS+  L   KMT
Sbjct: 189 NHLEGKWHTSSKIMTRSRRN-------------------YQIAQRAMPYSKGSL--EKMT 248

Query: 253 AKVKNVLCRAQGRQHFDVLLKHSRSLLR 279
             VKN+L +AQG Q    L+ HS SLLR
Sbjct: 249 QNVKNLLSKAQGNQTLQSLINHSHSLLR 255

[Close]

BLAST of Sed0005278 vs. TAIR 10
Match: AT1G74390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 260.8 bits (665), Expect = 1.3e-69
Identity = 135/233 (57.94%), Postives = 172/233 (73.82%), Query Frame = 0


Query: 3   ASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKDLS 62
           ASS   +  +EI FFDLET VP + G+ F +LEFGAI+VCP +L EL S++TL+RP DLS
Sbjct: 2   ASSLGGDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLS 61

Query: 63  VVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIG 122
           +++  + RR GITR+ V +A  F E+ADK++ IL+GRIWAGHNI RFDCVRIREAFAEIG
Sbjct: 62  LISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIG 121

Query: 123 RPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKHCA 182
              P+P   +DSL +L+QKFGKRAG+MKMAS+A+YF LG Q HRSLDDVR+NLEV+K+CA
Sbjct: 122 LSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCA 181

Query: 183 TVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCR-----EESSRKSPPSSP 231
           TVLFLES++P IL+     S   + R R+NG+L          SS  SP + P
Sbjct: 182 TVLFLESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDP 234

[Close]

BLAST of Sed0005278 vs. TAIR 10
Match: AT5G07710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 1.7e-69
Identity = 129/202 (63.86%), Postives = 159/202 (78.71%), Query Frame = 0


Query: 9   EGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKDLSVVAERS 68
           E  +EI FFD+ETTVP R GQRF +LEFG+I+VCP KL EL S+TTL++P DLS+++  S
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 69  GRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPKP 128
            R  GI R+ V  APLF ++AD ++ IL+GRIWAGHNI RFDC RIREAFAEIGR  P+P
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 129 VGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKHCATVLFLE 188
            G +DSLG+LTQKFG+RAG+MKMA++A YF LG Q HRSLDDVR+NLEVLK+CATVLFLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 189 STLPSILSGKWKTSSTITTRNR 211
           S+LP        +  TI++R R
Sbjct: 184 SSLPYAHVDNSVSPETISSRRR 204

[Close]

BLAST of Sed0005278 vs. TAIR 10
Match: AT5G61390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 7.8e-67
Identity = 126/222 (56.76%), Postives = 167/222 (75.23%), Query Frame = 0


Query: 9   EGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKDLSVVAERS 68
           E  +EI FFD+ETT+P RVGQ + +LEFG+I+VCP KLVEL++++ L+RP +L+++  RS
Sbjct: 11  EDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRS 70

Query: 69  GRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPKP 128
            +  GI RE V +A  F ++AD ++ IL+GRIWAGHNI +FDC RIREAFAEIGR  P+P
Sbjct: 71  VKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEP 130

Query: 129 VGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKHCATVLFLE 188
            G +DSL +LTQ+FG+RAG+MKMA++A+YF LG Q HRSLDDVR+N EVLK+CATVLFLE
Sbjct: 131 KGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLE 190

Query: 189 STLPSILSGKWKTSSTITTRNRANGRLHCREESSRKSPPSSP 231
           S+LP  L     T++T  T +R       R  + +KSP  SP
Sbjct: 191 SSLPDELIENSVTTTTPETSSR-------RRRTIKKSPLQSP 225

[Close]

BLAST of Sed0005278 vs. TAIR 10
Match: AT1G74390.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.3e-61
Identity = 126/233 (54.08%), Postives = 161/233 (69.10%), Query Frame = 0


Query: 3   ASSSFKEGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPKDLS 62
           ASS   +  +EI FFDLET VP + G+ F +LEFGAI+VCP +L EL S++TL+RP DLS
Sbjct: 2   ASSLGGDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLS 61

Query: 63  VVAERSGRREGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIG 122
           +++  + RR GITR+ V +A  F E+ADK++ IL+GRIWAGHNI RFDCVRIREAFAEIG
Sbjct: 62  LISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIG 121

Query: 123 RPAPKPVGMVDSLGVLTQKFGKRAGNMKMASMASYFQLGQQKHRSLDDVRLNLEVLKHCA 182
              P+P   +DSL +L+QKFGKRAG+MKMAS+A+YF LG Q HRSLDDVR+NLE      
Sbjct: 122 LSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLE------ 181

Query: 183 TVLFLESTLPSILSGKWKTSSTITTRNRANGRLHCR-----EESSRKSPPSSP 231
                ES++P IL+     S   + R R+NG+L          SS  SP + P
Sbjct: 182 -----ESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDP 223

The following BLAST results are available for this feature:

BLAST of Sed0005278 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133010.1	3.2e-139	91.37	protein NEN4 isoform X2 [Momordica charantia]	[more]
XP_023518830.1	7.1e-139	92.81	protein NEN4-like [Cucurbita pepo subsp. pepo]	[more]
XP_022962945.1	7.1e-139	92.81	protein NEN4-like [Cucurbita moschata]	[more]
KAG7026830.1	2.1e-138	92.45	Protein NEN4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6594865.1	4.6e-138	92.09	Protein NEN4, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Sed0005278 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JJ23	4.4e-91	65.67	Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1	[more]
Q9CA74	1.8e-68	57.94	Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1	[more]
Q9FLR0	2.3e-68	63.86	Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1	[more]
Q0V842	1.1e-65	56.76	Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1	[more]

BLAST of Sed0005278 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BU43	1.5e-139	91.37	protein NEN4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005707 PE=4 SV=1	[more]
A0A6J1HGK3	3.4e-139	92.81	protein NEN4-like OS=Cucurbita moschata OX=3662 GN=LOC111463299 PE=4 SV=1	[more]
A0A6J1BUQ8	8.5e-138	90.07	protein NEN4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005707 PE=4 SV=1	[more]
A0A6J1KYA7	1.9e-137	91.73	protein NEN4 OS=Cucurbita maxima OX=3661 GN=LOC111497460 PE=4 SV=1	[more]
A0A5D3CL58	4.4e-134	88.85	Protein NEN4 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of Sed0005278 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39810.1	3.1e-92	65.67	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G74390.1	1.3e-69	57.94	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G07710.1	1.7e-69	63.86	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G61390.1	7.8e-67	56.76	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G74390.2	1.3e-61	54.08	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013520	Exonuclease, RNase T/DNA polymerase III	SMART	SM00479	exoiiiendus	coord: 13..187 e-value: 1.9E-26 score: 103.9
IPR013520	Exonuclease, RNase T/DNA polymerase III	PFAM	PF00929	RNase_T	coord: 15..178 e-value: 8.3E-28 score: 98.0
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 6..185 e-value: 5.1E-30 score: 106.6
None	No IPR available	PANTHER	PTHR30231:SF26	PROTEIN NEN4	coord: 5..189
None	No IPR available	PANTHER	PTHR30231	DNA POLYMERASE III SUBUNIT EPSILON	coord: 5..189
None	No IPR available	CDD	cd06127	DEDDh	coord: 15..179 e-value: 1.67474E-24 score: 92.3636
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 14..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0005278.1	Sed0005278.1	mRNA
Sed0005278.2	Sed0005278.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding