Sed0004658 (gene) Chayote v1

BLAST of Sed0004658 vs. NCBI nr
Match: XP_023540992.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023540993.1 protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023540994.1 protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 963.8 bits (2490), Expect = 6.8e-277
Identity = 491/578 (84.95%), Postives = 529/578 (91.52%), Query Frame = 0

Query: 1   MASPNPSTGNRFWQQLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MA+ NPST +  W Q L GRWFS+FASILIMSVSGATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGLIGGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCL 120
           LSFFKDLGGNVG+IGGLINEVAPPWVVL IGAVMNLFGYTMIW+AVT RIP PQIWHMCL
Sbjct: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKS 180
           YICIGANSQTFANTGALVTSVKNFP+SRGSVLGLLKGFVGLSGAILTQ+YHAFYG+NSKS
Sbjct: 121 YICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKS 180

Query: 181 LILLIAWLPATVSVSFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMVLIILQNRLQ 240
           LILLIAWLPA VSV+FL FVR I DLR+PNEL+VF HILYISL LAGSLMVLIILQNRL+
Sbjct: 181 LILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLK 240

Query: 241 FRQMEYAGSAIVVVALLLLPLAVVYREELSIFKSKMENTISQLELAIASRQPPLSA---- 300
           F QMEYAGSAIVV+ALLLLPLA+VYREELS++K+K+E+ +SQLELA   + PPL++    
Sbjct: 241 FHQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPP 300

Query: 301 APPTSPAGSCIENTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIG 360
           APP+ P+GSC ENTFKPPNRGEDYTIPQA+FS+DMIIL  ATICGVGGTLTAIDNLGQIG
Sbjct: 301 APPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIG 360

Query: 361 ESLGYPSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLL 420
           ESL YPS STTTFISLVSIWNYLGRVVAGF SEFLWKKY+VPRPLI FV +ILSCVGHLL
Sbjct: 361 ESLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLL 420

Query: 421 IAFGVPNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNV 480
           IAFGVPNSLYF+SIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAY+LNV
Sbjct: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480

Query: 481 RVAGRLYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVR 540
           RVAG LYD EA RQM AAGRRR IGEDL+C+GVECYRKAFLIITAATV GAFVSL+LVVR
Sbjct: 481 RVAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVR 540

Query: 541 TWKFYKGDIYRKFRAEEGEDAEAKMAAPTNKAAAKMES 575
           TWKFYKGDIYRKFR E GE+ E +MAAPTN A AK ++
Sbjct: 541 TWKFYKGDIYRKFREEAGEEVEVQMAAPTNSAVAKSQT 578

BLAST of Sed0004658 vs. NCBI nr
Match: XP_022947808.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 963.8 bits (2490), Expect = 6.8e-277
Identity = 491/578 (84.95%), Postives = 529/578 (91.52%), Query Frame = 0

Query: 1   MASPNPSTGNRFWQQLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MA+ NPST +  W Q L GRWFS+FASILIMSVSGATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGLIGGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCL 120
           LSFFKDLGGNVG+IGGLINEVAPPWVVL IGAVMNLFGYTMIW+AVT RIP PQIWHMCL
Sbjct: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKS 180
           YICIGANSQTFANTGALVTSVKNFP+SRGSVLGLLKGFVGLSGAILTQ+YHAFYG+NSKS
Sbjct: 121 YICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKS 180

Query: 181 LILLIAWLPATVSVSFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMVLIILQNRLQ 240
           LILLIAWLPA VSV+FL FVR I DLR+PNEL+VF HILYISL LAGSLMVLIILQNRL+
Sbjct: 181 LILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLK 240

Query: 241 FRQMEYAGSAIVVVALLLLPLAVVYREELSIFKSKMENTISQLELAIASRQPPLSA---- 300
           F QMEYAGSAIVV+ALLLLPLA+VYREELS++K+K+E+ +SQLELA   + PPL++    
Sbjct: 241 FHQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPV 300

Query: 301 APPTSPAGSCIENTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIG 360
           APP+ P+GSC ENTFKPPNRGEDYTIPQA+FS+DMIIL  ATICGVGGTLTAIDNLGQIG
Sbjct: 301 APPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIG 360

Query: 361 ESLGYPSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLL 420
           ESL YPS STTTFISLVSIWNYLGRVVAGF SEFLWKKY+VPRPLI FV +ILSCVGHLL
Sbjct: 361 ESLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLL 420

Query: 421 IAFGVPNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNV 480
           IAFGVPNSLYF+SIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAY+LNV
Sbjct: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480

Query: 481 RVAGRLYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVR 540
           RVAG LYD EA RQM AAGRRR IGEDL+C+GVECYRKAFLIITAATV GAFVSL+LVVR
Sbjct: 481 RVAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVR 540

Query: 541 TWKFYKGDIYRKFRAEEGEDAEAKMAAPTNKAAAKMES 575
           TWKFYKGDIYRKFR E GE+ E +MAAPTN A AK ++
Sbjct: 541 TWKFYKGDIYRKFREEAGEEVEVQMAAPTNSAVAKSQT 578

BLAST of Sed0004658 vs. NCBI nr
Match: KAG6596491.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 962.6 bits (2487), Expect = 1.5e-276
Identity = 490/578 (84.78%), Postives = 529/578 (91.52%), Query Frame = 0

Query: 1   MASPNPSTGNRFWQQLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MA+ NPST +  W Q L GRWFS+FASILIMSVSGATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGLIGGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCL 120
           LSFFKDLGGNVG+IGGLINEVAPPWVVL IGAVMNLFGYTMIW+AVT RIP PQIWHMCL
Sbjct: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKS 180
           YICIGANSQTFANTGALVTSVKNFP+SRGSVLGLLKGFVGLSGAILTQ+YHAFYG+NSKS
Sbjct: 121 YICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKS 180

Query: 181 LILLIAWLPATVSVSFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMVLIILQNRLQ 240
           LILLIAWLPA VSV+FL FVR I DLR+PNEL+VF HILYISL LAGSLMVLIILQNRL+
Sbjct: 181 LILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLK 240

Query: 241 FRQMEYAGSAIVVVALLLLPLAVVYREELSIFKSKMENTISQLELAIASRQPPLSA---- 300
           F QMEYAGSAIVV+ALLLLPLA+VYREELS++K+K+E+ +SQLELA   + PPL++    
Sbjct: 241 FHQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPL 300

Query: 301 APPTSPAGSCIENTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIG 360
           APP+ P+GSC ENTFKPPNRGEDYTIPQA+FS+DMIIL  ATICGVGGTLTAIDNLGQIG
Sbjct: 301 APPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIG 360

Query: 361 ESLGYPSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLL 420
           ESL YPS STTTFISLVSIWNYLGRVVAGF SEFLWKKY+VPRPLI FV +ILSCVGHLL
Sbjct: 361 ESLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLL 420

Query: 421 IAFGVPNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNV 480
           IAFGVPNSLYF+SIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAY+LNV
Sbjct: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480

Query: 481 RVAGRLYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVR 540
           +VAG LYD EA RQM AAGRRR IGEDL+C+GVECYRKAFLIITAATV GAFVSL+LVVR
Sbjct: 481 KVAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVR 540

Query: 541 TWKFYKGDIYRKFRAEEGEDAEAKMAAPTNKAAAKMES 575
           TWKFYKGDIYRKFR E GE+ E +MAAPTN A AK ++
Sbjct: 541 TWKFYKGDIYRKFREEAGEEVEVQMAAPTNSAVAKSQT 578

BLAST of Sed0004658 vs. NCBI nr
Match: XP_023005202.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 958.0 bits (2475), Expect = 3.7e-275
Identity = 489/578 (84.60%), Postives = 528/578 (91.35%), Query Frame = 0

Query: 1   MASPNPSTGNRFWQQLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MA+ NPST +  W Q L GRWFS+FASILIMSVSGATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGLIGGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCL 120
           LSFFKDLGGNVG+IGGLINEVAPPWVVL IGAVMNLFGYTMIW+AVT RIP PQIWHMCL
Sbjct: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKS 180
           YICIGANSQTFANTGALVTSVKNFP+SRGSVLGLLKGFVGLSGAILTQ+YHAFYG+NSKS
Sbjct: 121 YICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKS 180

Query: 181 LILLIAWLPATVSVSFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMVLIILQNRLQ 240
           LILLIAWLPA VSV+FL FVR I DLR+PNEL+VF HILYISL LAGSLMVLIILQNRL+
Sbjct: 181 LILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLK 240

Query: 241 FRQMEYAGSAIVVVALLLLPLAVVYREELSIFKSKMENTISQLELAIASRQPPLSA---- 300
           F QMEYAGSAIVV+ALLLLPLA+VYREELS++K+K+E+ ISQLELA   + PPL++    
Sbjct: 241 FHQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPISQLELASQPQPPPLTSAAPV 300

Query: 301 APPTSPAGSCIENTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIG 360
           APP+ P+GSC ENTFKPPNRGEDYTI QA+FS+DMIIL  ATICGVGGTLTAIDNLGQIG
Sbjct: 301 APPSPPSGSCFENTFKPPNRGEDYTILQAVFSIDMIILFIATICGVGGTLTAIDNLGQIG 360

Query: 361 ESLGYPSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLL 420
           ESL YPS STTTFISLVSIWNYLGRVVAGF SEFLWKKY+VPRPLI FV +ILSCVGHLL
Sbjct: 361 ESLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLL 420

Query: 421 IAFGVPNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNV 480
           IAFGVPNSLYF+SIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAY+LNV
Sbjct: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480

Query: 481 RVAGRLYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVR 540
           RVAG LYD EA RQM AAGRRR IGEDL+C+GVECYRKAFLIITAATV GAFVSL+LVVR
Sbjct: 481 RVAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVR 540

Query: 541 TWKFYKGDIYRKFRAEEGEDAEAKMAAPTNKAAAKMES 575
           TWKFYKGDIY+KFR + GE+ E +MAAPTN A AK ++
Sbjct: 541 TWKFYKGDIYQKFREDSGEEVEVQMAAPTNSAVAKSQT 578

BLAST of Sed0004658 vs. NCBI nr
Match: XP_023522337.1 (protein NUCLEAR FUSION DEFECTIVE 4-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 942.2 bits (2434), Expect = 2.1e-270
Identity = 479/556 (86.15%), Postives = 513/556 (92.27%), Query Frame = 0

Query: 1   MASPNPSTGNRFWQQLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MA+ NPST +  W Q L GRWFS+FASILIMSVSGATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGLIGGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCL 120
           LSFFKDLGGNVG+IGGLINEVAPPWVVL IGAVMNLFGYTMIW+AVT RIP PQIWHMCL
Sbjct: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKS 180
           YICIGANSQTFANTGALVTSVKNFP+SRGSVLGLLKGFVGLSGAILTQ+YHAFYG+NSKS
Sbjct: 121 YICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKS 180

Query: 181 LILLIAWLPATVSVSFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMVLIILQNRLQ 240
           LILLIAWLPA VSV+FL FVR I DLR+PNEL+VF HILYISL LAGSLMVLIILQNRL+
Sbjct: 181 LILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLK 240

Query: 241 FRQMEYAGSAIVVVALLLLPLAVVYREELSIFKSKMENTISQLELAIASRQPPLSA---- 300
           F QMEYAGSAIVV+ALLLLPLA+VYREELS++K+K+E+ +SQLELA   + PPL++    
Sbjct: 241 FHQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPP 300

Query: 301 APPTSPAGSCIENTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIG 360
           APP+ P+GSC ENTFKPPNRGEDYTIPQA+FS+DMIIL  ATICGVGGTLTAIDNLGQIG
Sbjct: 301 APPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIG 360

Query: 361 ESLGYPSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLL 420
           ESL YPS STTTFISLVSIWNYLGRVVAGF SEFLWKKY+VPRPLI FV +ILSCVGHLL
Sbjct: 361 ESLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLL 420

Query: 421 IAFGVPNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNV 480
           IAFGVPNSLYF+SIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAY+LNV
Sbjct: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480

Query: 481 RVAGRLYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVR 540
           RVAG LYD EA RQM AAGRRR IGEDL+C+GVECYRKAFLIITAATV GAFVSL+LVVR
Sbjct: 481 RVAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVR 540

Query: 541 TWKFYKGDIYRKFRAE 553
           TWKFYKGDIYRKFR E
Sbjct: 541 TWKFYKGDIYRKFREE 556

BLAST of Sed0004658 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 9.0e-30
Identity = 133/542 (24.54%), Postives = 251/542 (46.31%), Query Frame = 0

Query: 17  LFGRWFS---IFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGL 76
           +FGRW     + A+I I + +G  + F  YSS +KS L   Q  LN L+   DLG   G 
Sbjct: 37  MFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGW 96

Query: 77  IGGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCLYIC--IGANSQTF 136
             G+     P  VVL   A M   GY + WL +T  I  P   +  +++C  +   S  +
Sbjct: 97  SSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP---YSLVFLCCLLAGLSICW 156

Query: 137 ANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKSLILLIAWLPAT 196
            NT   +  +++FP +R   L L   F G+S A+ +  ++A   S+S   +LL + +P  
Sbjct: 157 FNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLV 216

Query: 197 VSVSFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMV----LIILQNRLQFRQMEYA 256
           VS + L  V     L    +     H  ++   L    ++    L++  +     ++ + 
Sbjct: 217 VSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFI 276

Query: 257 GSAIVVVALLLLPLAVVYREE-LSIFKSKMENTISQLEL----AIASRQPPLSAAPPTSP 316
           G+ +++V  L  PL V  R+  L +  +++ +  S   +     + +++  +S+      
Sbjct: 277 GAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEH 336

Query: 317 AGSCIE-NTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIGESLGY 376
            G+  E NT +    G++++    I  ++  +   A  CG    L   +NLGQI +SLG 
Sbjct: 337 MGTAKEGNTVR---LGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG- 396

Query: 377 PSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLLIAF-- 436
              ++TT +++ S +++ GR+++  + +F+ K++R+ R     + ++ + +   L+A   
Sbjct: 397 --QNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSS 456

Query: 437 GVPNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNVRVA 496
               +L  A+ +IG   G  +    +I S++FG       +N+     PIG+ +L   +A
Sbjct: 457 SQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIA 516

Query: 497 GRLYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVRTWK 542
             +Y+  A   +        + + + C+G +CY K F+     ++ G   SL L +RT  
Sbjct: 517 ASIYEANASPDITPI-----VSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKP 562

BLAST of Sed0004658 vs. ExPASy TrEMBL
Match: A0A6J1G7M8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451560 PE=4 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.3e-277
Identity = 491/578 (84.95%), Postives = 529/578 (91.52%), Query Frame = 0

Query: 1   MASPNPSTGNRFWQQLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MA+ NPST +  W Q L GRWFS+FASILIMSVSGATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGLIGGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCL 120
           LSFFKDLGGNVG+IGGLINEVAPPWVVL IGAVMNLFGYTMIW+AVT RIP PQIWHMCL
Sbjct: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKS 180
           YICIGANSQTFANTGALVTSVKNFP+SRGSVLGLLKGFVGLSGAILTQ+YHAFYG+NSKS
Sbjct: 121 YICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKS 180

Query: 181 LILLIAWLPATVSVSFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMVLIILQNRLQ 240
           LILLIAWLPA VSV+FL FVR I DLR+PNEL+VF HILYISL LAGSLMVLIILQNRL+
Sbjct: 181 LILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLK 240

Query: 241 FRQMEYAGSAIVVVALLLLPLAVVYREELSIFKSKMENTISQLELAIASRQPPLSA---- 300
           F QMEYAGSAIVV+ALLLLPLA+VYREELS++K+K+E+ +SQLELA   + PPL++    
Sbjct: 241 FHQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPV 300

Query: 301 APPTSPAGSCIENTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIG 360
           APP+ P+GSC ENTFKPPNRGEDYTIPQA+FS+DMIIL  ATICGVGGTLTAIDNLGQIG
Sbjct: 301 APPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIG 360

Query: 361 ESLGYPSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLL 420
           ESL YPS STTTFISLVSIWNYLGRVVAGF SEFLWKKY+VPRPLI FV +ILSCVGHLL
Sbjct: 361 ESLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLL 420

Query: 421 IAFGVPNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNV 480
           IAFGVPNSLYF+SIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAY+LNV
Sbjct: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480

Query: 481 RVAGRLYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVR 540
           RVAG LYD EA RQM AAGRRR IGEDL+C+GVECYRKAFLIITAATV GAFVSL+LVVR
Sbjct: 481 RVAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVR 540

Query: 541 TWKFYKGDIYRKFRAEEGEDAEAKMAAPTNKAAAKMES 575
           TWKFYKGDIYRKFR E GE+ E +MAAPTN A AK ++
Sbjct: 541 TWKFYKGDIYRKFREEAGEEVEVQMAAPTNSAVAKSQT 578

BLAST of Sed0004658 vs. ExPASy TrEMBL
Match: A0A6J1KSH4 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111498302 PE=4 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 1.8e-275
Identity = 489/578 (84.60%), Postives = 528/578 (91.35%), Query Frame = 0

Query: 1   MASPNPSTGNRFWQQLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNL 60
           MA+ NPST +  W Q L GRWFS+FASILIMSVSGATYMFGLYSSDIKSSL YDQTTLNL
Sbjct: 1   MAASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNL 60

Query: 61  LSFFKDLGGNVGLIGGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCL 120
           LSFFKDLGGNVG+IGGLINEVAPPWVVL IGAVMNLFGYTMIW+AVT RIP PQIWHMCL
Sbjct: 61  LSFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCL 120

Query: 121 YICIGANSQTFANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKS 180
           YICIGANSQTFANTGALVTSVKNFP+SRGSVLGLLKGFVGLSGAILTQ+YHAFYG+NSKS
Sbjct: 121 YICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKS 180

Query: 181 LILLIAWLPATVSVSFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMVLIILQNRLQ 240
           LILLIAWLPA VSV+FL FVR I DLR+PNEL+VF HILYISL LAGSLMVLIILQNRL+
Sbjct: 181 LILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLK 240

Query: 241 FRQMEYAGSAIVVVALLLLPLAVVYREELSIFKSKMENTISQLELAIASRQPPLSA---- 300
           F QMEYAGSAIVV+ALLLLPLA+VYREELS++K+K+E+ ISQLELA   + PPL++    
Sbjct: 241 FHQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPISQLELASQPQPPPLTSAAPV 300

Query: 301 APPTSPAGSCIENTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIG 360
           APP+ P+GSC ENTFKPPNRGEDYTI QA+FS+DMIIL  ATICGVGGTLTAIDNLGQIG
Sbjct: 301 APPSPPSGSCFENTFKPPNRGEDYTILQAVFSIDMIILFIATICGVGGTLTAIDNLGQIG 360

Query: 361 ESLGYPSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLL 420
           ESL YPS STTTFISLVSIWNYLGRVVAGF SEFLWKKY+VPRPLI FV +ILSCVGHLL
Sbjct: 361 ESLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLL 420

Query: 421 IAFGVPNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNV 480
           IAFGVPNSLYF+SIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAY+LNV
Sbjct: 421 IAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNV 480

Query: 481 RVAGRLYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVR 540
           RVAG LYD EA RQM AAGRRR IGEDL+C+GVECYRKAFLIITAATV GAFVSL+LVVR
Sbjct: 481 RVAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVR 540

Query: 541 TWKFYKGDIYRKFRAEEGEDAEAKMAAPTNKAAAKMES 575
           TWKFYKGDIY+KFR + GE+ E +MAAPTN A AK ++
Sbjct: 541 TWKFYKGDIYQKFREDSGEEVEVQMAAPTNSAVAKSQT 578

BLAST of Sed0004658 vs. ExPASy TrEMBL
Match: A0A5A7TKH4 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002830 PE=4 SV=1)

HSP 1 Score: 915.2 bits (2364), Expect = 1.3e-262
Identity = 463/569 (81.37%), Postives = 511/569 (89.81%), Query Frame = 0

Query: 2   ASPNPSTGNRFWQQLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLL 61
           ++ NP++ N FW Q LFGRWFS+FASILIMSV+GATYMF LYSSDIKSSL YDQTTLNLL
Sbjct: 4   STSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLL 63

Query: 62  SFFKDLGGNVGLIGGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCLY 121
           SFFKDLGGNVG+I GLINEVAP WVVL IGAVMNLFGYTMIWLAVT RIP PQIWHMCLY
Sbjct: 64  SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY 123

Query: 122 ICIGANSQTFANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKSL 181
           IC+GANSQTFANTGALVT VKNFP+SRGSVLGLLKGFVGLSGAIL+Q++HAFYG++SKSL
Sbjct: 124 ICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSL 183

Query: 182 ILLIAWLPATVSVSFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMVLIILQNRLQF 241
           ILLI WLPA VSV FL FVR I DLR+PNE+ VF HILYISL LAG+LMVLIILQ+ L+F
Sbjct: 184 ILLIGWLPAAVSVVFLRFVRIIKDLRQPNEVNVFYHILYISLSLAGTLMVLIILQSLLRF 243

Query: 242 RQMEYAGSAIVVVALLLLPLAVVYREELSIFKSKMENTISQLELAIASRQPPLSA----A 301
           +Q++Y GSAIVV+ LLLLPLA+V+REELS++KSK+ N I QLELA     PPL++    A
Sbjct: 244 QQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTVPLA 303

Query: 302 PPTSPAGSCIENTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIGE 361
           P + P+ SC +N FKPPNRGEDYTIPQAIFS+DMIIL  ATICGVGGTLTAIDNLGQIGE
Sbjct: 304 PSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGE 363

Query: 362 SLGYPSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLLI 421
           SL YPSHSTTTFISLVSIWNYLGRVV+GF SE+ WKKY+VPRPL LFV +ILSC+GHLLI
Sbjct: 364 SLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLI 423

Query: 422 AFGVPNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNVR 481
           AFGVPNSLYF+SIIIGFCFGAQWPLIFAIISEIFGLKYYATLYN+GG ASPIGAY++NVR
Sbjct: 424 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVR 483

Query: 482 VAGRLYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVRT 541
           VAG LYD EA+RQM AAGR R+ GEDLSC+GVECYRKAFLIITAATVFGA VSL+LVVRT
Sbjct: 484 VAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRT 543

Query: 542 WKFYKGDIYRKFRAEEGEDAEAKMAAPTN 567
           WKFYKGDIYR+FRAEEGED E KMAAPTN
Sbjct: 544 WKFYKGDIYRRFRAEEGEDIEMKMAAPTN 572

BLAST of Sed0004658 vs. ExPASy TrEMBL
Match: A0A5A7TKY5 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002840 PE=4 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.9e-253
Identity = 447/569 (78.56%), Postives = 497/569 (87.35%), Query Frame = 0

Query: 7   STGNRFWQQLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLLSFFKD 66
           ++GN FW  LLFGRWFS+FASILIMSVSGATYMFGLYSS IKSSLAYDQTTLNLLSFFKD
Sbjct: 3   TSGNPFWYHLLFGRWFSVFASILIMSVSGATYMFGLYSSHIKSSLAYDQTTLNLLSFFKD 62

Query: 67  LGGNVGLIGGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCLYICIGA 126
           LG N+G+I GL+NEVAP WVVL IG VMNLFGYTMIWLAVT RIPNPQIWHMCLYICIGA
Sbjct: 63  LGANIGVISGLVNEVAPAWVVLLIGVVMNLFGYTMIWLAVTNRIPNPQIWHMCLYICIGA 122

Query: 127 NSQTFANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKSLILLIA 186
           NSQTFANT AL+T VKNFP+SRGS+LGL KGFVGLSGAIL+Q++HAFYG+NSKSLI LIA
Sbjct: 123 NSQTFANTAALITCVKNFPESRGSILGLFKGFVGLSGAILSQLFHAFYGNNSKSLIFLIA 182

Query: 187 WLPATVSVSFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMVLIILQNRLQFRQMEY 246
           WLP+ VSV    FVR I DLR+PNELKVF H+LYISL LAGSLMV IILQNRL+F+Q+ Y
Sbjct: 183 WLPSAVSVILHRFVRIIKDLRQPNELKVFYHVLYISLGLAGSLMVCIILQNRLRFQQIHY 242

Query: 247 AGSAIVVVALLLLPLAVVYREELSIFKSKMENTISQLELAIASRQPPLSAAPPTSPAGSC 306
            GSAIVV+ LLLLPLA+V+REEL I++SK++N I QLELA     PP    PP  P+ SC
Sbjct: 243 VGSAIVVIVLLLLPLAIVFREELRIWRSKIKNPIPQLELASQLPPPP----PPNPPSDSC 302

Query: 307 IENTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIGESLGYPSHST 366
            +N F PPNRGEDYTIPQAIFS+DMIIL  ATICGVGGTLTAIDNLGQIGESL YPSHST
Sbjct: 303 FKNMFNPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYPSHST 362

Query: 367 TTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLLIAFGVPNSLY 426
           TTFISLVSIWNYLGRVV+GF SE+ WKKY+VPRPL LFV +ILSC+GHLLIAFGVPNSLY
Sbjct: 363 TTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVPNSLY 422

Query: 427 FASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNVRVAGRLYDEE 486
           F+SIIIGFCFGAQWPLIFAIISEIFGLKYYATLYN+GG ASPIGAY++NVRVAG LYD E
Sbjct: 423 FSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHLYDME 482

Query: 487 ARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVRTWKFYKGDIY 546
           A RQM AAGR R+ GEDLSC+GVECY+KAFLIIT +TV G  VSL+LVVRTWKFYK DIY
Sbjct: 483 AERQMEAAGRLRKEGEDLSCLGVECYKKAFLIITGSTVLGGLVSLILVVRTWKFYKDDIY 542

Query: 547 RKFRAEEGEDAEAKMAAPTNKAAAKMESV 576
           R+F+ +EGED E K+AAPTN +    ++V
Sbjct: 543 RRFKEKEGEDIEIKIAAPTNNSLTAAKTV 567

BLAST of Sed0004658 vs. ExPASy TrEMBL
Match: A0A0A0LEA4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G742600 PE=4 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 5.6e-253
Identity = 452/569 (79.44%), Postives = 497/569 (87.35%), Query Frame = 0

Query: 2   ASPNPSTGNRFWQQLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLL 61
           +S NP++ N FW Q LFGRWFS+ ASILIMSV+GATYMF LYSSDIKSSL YDQTTLNLL
Sbjct: 4   SSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLL 63

Query: 62  SFFKDLGGNVGLIGGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCLY 121
           SFFKDLGGNVG+I GL NEVAP WVVL IGAVMNLFGYTMIWLAVTKRIP PQIWHMCLY
Sbjct: 64  SFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLY 123

Query: 122 ICIGANSQTFANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKSL 181
           I IGANSQTFANTGALVT VKNFP+SRGSVLGLLKGFVGLSGAIL+Q+Y AFYG+N +SL
Sbjct: 124 ITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESL 183

Query: 182 ILLIAWLPATVSVSFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMVLIILQNRLQF 241
           ILLIAWLPA VSV  L FVR I DLR+PNELKVF H LYISL LAG+LMVLIILQ+ L+F
Sbjct: 184 ILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRF 243

Query: 242 RQMEYAGSAIVVVALLLLPLAVVYREELSIFKSKMENTISQLELAIASRQPPL----SAA 301
           +Q++Y GSAIVV+ LLLLPL +V+REELS++KSK+ + + QLE A     PPL    S A
Sbjct: 244 QQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSLA 303

Query: 302 PPTSPAGSCIENTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIGE 361
           P + P+ SC +N F PP+RGEDYTIPQAIFS+D+IIL  ATICGVGGTLTAIDNLGQIGE
Sbjct: 304 PSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGE 363

Query: 362 SLGYPSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLLI 421
           SLGY SHS TTFISLVSIWNYLGRVV+GF SE+ WKKY+VPRPL LF  +ILSCVGHLLI
Sbjct: 364 SLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 423

Query: 422 AFGVPNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNVR 481
           AFGVPNSLYF+SI+IGFCFGAQWPLI+AIISEIFGLKYYATL ++ G ASPIGAY+LNVR
Sbjct: 424 AFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVR 483

Query: 482 VAGRLYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVRT 541
           VAG LYD EA+RQM A GRRR IGEDLSC+GVECYRKAFLIITAATVFGA VSL+LVVRT
Sbjct: 484 VAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRT 543

Query: 542 WKFYKGDIYRKFRAEEGEDAEAKMAAPTN 567
           WKFYKGDIYRKFR  EGED E KMAAPTN
Sbjct: 544 WKFYKGDIYRKFREVEGEDIEMKMAAPTN 572

BLAST of Sed0004658 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 682.2 bits (1759), Expect = 3.7e-196
Identity = 352/563 (62.52%), Postives = 425/563 (75.49%), Query Frame = 0

Query: 15  QLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGLI 74
           Q+L GRWF  F S+LIMS +GATYMFG+YS DIK +L YDQTTLNLLSFFKDLG NVG++
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 75  GGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCLYICIGANSQTFANT 134
            GL+NEV PPW +L IGA++N FGY MIWLAVT+RI  PQ+WHMCLYIC+GANSQ+FANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 135 GALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKSLILLIAWLPATVSV 194
           G+LVT VKNFP+SRG VLG+LKG+VGLSGAI+TQ+Y AFYG ++K LIL+I WLPA VS 
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVSF 195

Query: 195 SFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMVLIILQNRLQFRQMEYAGSAIVVV 254
           +FL  +R +   R+ NELKVF + LYISL LA  LMV+II+     F Q E+ GSA VV+
Sbjct: 196 AFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVI 255

Query: 255 ALLLLPLAVVYREELSIFKSKMENTISQLELAIASRQPPLSAAPPTSPAG---------- 314
            LLLLP+ VV  EE  ++K K         + + + +P L ++      G          
Sbjct: 256 VLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEKV 315

Query: 315 ---SCIENTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIGESLGY 374
              SC    F PP RG+DYTI QA+FSVDM+IL  ATICGVGGTLTAIDNLGQIG SLGY
Sbjct: 316 KTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLGY 375

Query: 375 PSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLLIAFGV 434
           P  S +TF+SLVSIWNY GRVV+G  SE    KY+ PRPL+L +V++LSC GHLLIAF V
Sbjct: 376 PKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNV 435

Query: 435 PNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNVRVAGR 494
           P  LY AS+IIGFCFGAQWPL+FAIISEIFGLKYY+TLYN G VASPIG+Y+LNVRVAG 
Sbjct: 436 PGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAGY 495

Query: 495 LYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVRTWKFY 554
           LYD EA +Q  A G+ R  G+DL+C+G  C++ +F+II A T+FG  VS+VLV+RT KFY
Sbjct: 496 LYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKFY 555

Query: 555 KGDIYRKFRAEEGEDAEAKMAAP 565
           K DIY+KFR E+   AE +MAAP
Sbjct: 556 KSDIYKKFR-EKALAAEMEMAAP 577

BLAST of Sed0004658 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 625.9 bits (1613), Expect = 3.1e-179
Identity = 325/569 (57.12%), Postives = 413/569 (72.58%), Query Frame = 0

Query: 11  RFWQQLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLLSFFKDLGGN 70
           RF      GRWF +FAS LIM+ +GATY+FG YS DIKS+L YDQTTLNLL FFKDLG N
Sbjct: 8   RFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGAN 67

Query: 71  VGLIGGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCLYICIGANSQT 130
           VG++ GLI EV P W VL IG+ MN  GY MIWL VT ++  P++W MCLYICIGANSQ 
Sbjct: 68  VGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQN 127

Query: 131 FANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKSLILLIAWLPA 190
           FANTGALVT VKNFP+SRG +LGLLKG+VGLSGAI TQ+Y A YG +SKSLILLIAWLPA
Sbjct: 128 FANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPA 187

Query: 191 TVSVSFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMVLIILQNRLQFRQMEYAGSA 250
            VS+ F+  +R+   +R+ NEL VF   LYIS+FLA  LM + I + ++ F +  YA SA
Sbjct: 188 AVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASA 247

Query: 251 IVVVALLLLPLAVVYREELSIF-----------KSKMENTISQLELAIASRQPPLSAAPP 310
            +  ALL +PL V  ++EL ++           + K+E    +L+L    +         
Sbjct: 248 TICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDL---DQDKAAKVNGE 307

Query: 311 TSPAGSCIENTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIGESL 370
                SC    F PP RGEDYTI QA+ S DMIIL  AT CG+G +LTA+DNLGQIGESL
Sbjct: 308 EKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESL 367

Query: 371 GYPSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLLIAF 430
           GYP+H+ ++F+SLVSIWNY GRV +GF SE+L  KY++PRPL++ +V++LSC GHLLIAF
Sbjct: 368 GYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAF 427

Query: 431 GVPNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNVRVA 490
            VP S+Y ASI++GF FGAQ PL+FAIISE+FGLKYY+TL+N G +ASP+G+Y+LNVRV 
Sbjct: 428 PVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVT 487

Query: 491 GRLYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVRTWK 550
           G LYD+EA +Q+ A G  R+  +DL+C+G +CY+  FLI+ A T FGA VSL L +RT +
Sbjct: 488 GMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTRE 547

Query: 551 FYKGDIYRKFRAEEGEDAEAKMAAPTNKA 569
           FYKGDIY+KFR  E  ++E+++   + KA
Sbjct: 548 FYKGDIYKKFR--ESPESESELVPDSRKA 571

BLAST of Sed0004658 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 333.2 bits (853), Expect = 4.2e-91
Identity = 206/581 (35.46%), Postives = 312/581 (53.70%), Query Frame = 0

Query: 7   STGNRFWQQLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLLSFFKD 66
           ST    +   +  RW    A++ I S +G  Y+FG  S  IKSSL Y+Q  L+ L   KD
Sbjct: 3   STTREKFVSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKD 62

Query: 67  LGGNVGLIGGLINEVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCLYICIGA 126
           LG +VG I G ++E+ P W  L +GAV NL GY  +WL VT R P   +W MC+ I +G 
Sbjct: 63  LGDSVGFIAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGN 122

Query: 127 NSQTFANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKSLILLIA 186
           N +T+ NTGALV+ V+NFP+SRG V+G+LKGF GL GAI++Q+Y   + SN  SLIL++A
Sbjct: 123 NGETYFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVA 182

Query: 187 WLPATVSVSFLCFVRKINDLR--RPNELKVFLHILYISLFLAGSLMVLIILQNRLQFRQM 246
             PA V V  + F+R +   +  RP +   F  I  + L LA  LM ++++Q+ +     
Sbjct: 183 VTPAVVVVCLMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHN 242

Query: 247 EYAGSAIVVVALLLLPLAVVYREELSIFKSKMENTISQLELAIASRQPP--------LSA 306
                 IV+  +L++P+ V          ++ ++TI +  +     Q P        LS 
Sbjct: 243 VITVFTIVLFVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSE 302

Query: 307 APPTSP---------------------------AGSCIENTFKPPNRGEDYTIPQAIFSV 366
                P                            G+   N  + P+RGED+T+ QA+   
Sbjct: 303 VEDEKPKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKA 362

Query: 367 DMIILITATICGVGGTLTAIDNLGQIGESLGYPSHSTTTFISLVSIWNYLGRVVAGFSSE 426
           D  ++  + + G G  LT IDNLGQ+ +SLGY   +T   +S++SIWN+LGR+  G+ SE
Sbjct: 363 DFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGY--DNTHVLVSMISIWNFLGRIGGGYFSE 422

Query: 427 FLWKKYRVPRPLILFVVVILSCVGHLLIAFGVPNSLYFASIIIGFCFGAQWPLIFAIISE 486
            + + Y  PRP+ + V  ++  VGH+  A+G P ++Y  +++IG  +GA W ++ A  SE
Sbjct: 423 LVVRDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASE 482

Query: 487 IFGLKYYATLYNLGGVASPIGAYVLNVRVAGRLYDEEARRQMAAAGRRREIGEDLSCVGV 546
           +FGLK +  LYN   +A+P G+ V +  +A  +YD EA RQ  A G   +  + L C G 
Sbjct: 483 LFGLKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQ--AHGSVFDPDDALRCNGS 542

Query: 547 ECYRKAFLIITAATVFGAFVSLVLVVRTWKFYKGDIYRKFR 551
            C+    LI++   +    +S++LV RT   Y   +Y K R
Sbjct: 543 ICFFLTSLIMSGFCIIACMLSMILVRRTKSVYT-HLYGKTR 578

BLAST of Sed0004658 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 325.9 bits (834), Expect = 6.7e-89
Identity = 197/544 (36.21%), Postives = 307/544 (56.43%), Query Frame = 0

Query: 15  QLLFGRWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGLI 74
           ++L  +W ++ ASI I   SGA+Y FG+YS+ +KS+ +YDQ+TL+ +S FKD+G N G+ 
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 75  GGLINEVAP----------------PWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHM 134
            GL+   A                 PWVVLA+GA+    GY +IW +VT  I  P +  M
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 135 CLYICIGANSQTFANTGALVTSVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNS 194
           CL++ + A SQTF NT  +V++V+NF    G+ +G++KGF+GLSGAIL Q+Y      + 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDP 181

Query: 195 KSLILLIAWLPATVSVSFLCFVRKINDLRRPNELKVFLHILYISLFLAGSLMVLIILQNR 254
            S ILL+A  P  +S+  +  VR I +    ++ K    +  +SL +A  LM++IIL+N 
Sbjct: 182 ASFILLLAVTPTVLSLLVMPLVR-IYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNT 241

Query: 255 LQFRQMEYAGSAIVVVALLLLPLAVVYREELSIFKSKMENTISQLELAIASRQPPLSAAP 314
                     + + ++ +L LPL +  R +    +  ME T+      + S     ++  
Sbjct: 242 FGLSSWANIVTLVCLLVMLALPLLIARRAQ----RDGMEKTVPHDYSPLISSPKATTSGN 301

Query: 315 PTSPAGSCIENTFKPPNRGEDYTIPQAIFSVDMIILITATICGVGGTLTAIDNLGQIGES 374
            +S   S +E         E+  + QA+  +   +L  A ICG+G  L+ I+N+ QIGES
Sbjct: 302 QSSEGDSKVE-----AGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGES 361

Query: 375 LGYPSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRVPRPLILFVVVILSCVGHLLIA 434
           L Y S    + +SL SIWN+LGR  AG++S+ L  K   PRPL++   +    +GHL+IA
Sbjct: 362 LRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIA 421

Query: 435 FGVPNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYVLNVRV 494
            G   +LY  S+I+G C+G+QW L+  I SE+FG+++  T++N   VASPIG+Y+ +VR+
Sbjct: 422 SGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRL 481

Query: 495 AGRLYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFLIITAATVFGAFVSLVLVVRTW 543
            G +YD+ A             GE  +C G  C+R +F+I+ +   FG  V++VL  RT 
Sbjct: 482 IGYIYDKTAS------------GEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTK 523

BLAST of Sed0004658 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 322.4 bits (825), Expect = 7.4e-88
Identity = 197/573 (34.38%), Postives = 306/573 (53.40%), Query Frame = 0

Query: 20  RWFSIFASILIMSVSGATYMFGLYSSDIKSSLAYDQTTLNLLSFFKDLGGNVGLIGGLIN 79
           RW    A++ I S +G  Y+FG  S  IKSSL Y+Q  L+ L   KDLG +VG + G ++
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAGTLS 75

Query: 80  EVAPPWVVLAIGAVMNLFGYTMIWLAVTKRIPNPQIWHMCLYICIGANSQTFANTGALVT 139
           E+ P W  L +G+V NL GY  +WL VT R P   +W MC+ I +G N +T+ NT ALV+
Sbjct: 76  EILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYFNTAALVS 135

Query: 140 SVKNFPQSRGSVLGLLKGFVGLSGAILTQVYHAFYGSNSKSLILLIAWLPATVSVSFLCF 199
            V+NFP+SRG V+G+LKGF GL GAIL+QVY   + S+  SLI ++A  P+ V V  + F
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPSVVVVPLMFF 195

Query: 200 VRKINDLR--RPNELKVFLHILYISLFLAGSLMVLIILQNRLQFRQMEYAGSAIVVVALL 259
           +R +   R  R ++   F  I  + + LA  LM ++++++ +           +V+ A+L
Sbjct: 196 IRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAFTVVLFAIL 255

Query: 260 LLPLAVVYREELSIFKSKMENTISQLELAIASRQPPLSAAPP------------------ 319
           L+P+ +          +   +T+ +  L     Q P  +  P                  
Sbjct: 256 LVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSEVEDEKPKE 315

Query: 320 ----------------------TSPAGSCIENTFKPPNRGEDYTIPQAIFSVDMIILITA 379
                                  +  G+      + P+RGED+T+ QA+   D  ++  +
Sbjct: 316 VDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKADFWLIFFS 375

Query: 380 TICGVGGTLTAIDNLGQIGESLGYPSHSTTTFISLVSIWNYLGRVVAGFSSEFLWKKYRV 439
            + G G  LT IDNLGQ+ +SLGY   +T  F+S++SIWN+LGR+  G+ SE + + Y  
Sbjct: 376 LLLGSGSGLTVIDNLGQMSQSLGY--DNTHVFVSMISIWNFLGRIGGGYFSELIVRDYAY 435

Query: 440 PRPLILFVVVILSCVGHLLIAFGVPNSLYFASIIIGFCFGAQWPLIFAIISEIFGLKYYA 499
           PRP+ + V  ++  VGH+  A+G P +++  +++IG  +GA W ++ A  SE+FGLK + 
Sbjct: 436 PRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASELFGLKKFG 495

Query: 500 TLYNLGGVASPIGAYVLNVRVAGRLYDEEARRQMAAAGRRREIGEDLSCVGVECYRKAFL 551
            LYN   +A+P G+ V +  +A  +YD EA RQ  A G      + L C G  CY    L
Sbjct: 496 ALYNFLTLANPAGSLVFSGLIASSIYDREAERQ--AQGSLFNPDDVLRCRGSICYFLTSL 555