Sed0004566 (gene) Chayote v1

BLAST of Sed0004566 vs. NCBI nr
Match: XP_038899064.1 (polygalacturonase At1g48100-like [Benincasa hispida])

HSP 1 Score: 898.7 bits (2321), Expect = 2.2e-257
Identity = 427/470 (90.85%), Postives = 444/470 (94.47%), Query Frame = 0

Query: 1   MSSFVLSIAFWFSFIFCLL-FSSQARWHHHTKLKHSHHHKHSYI--------SPSPSPSP 60
           MSSFVLS A WFSFI CLL  SSQARWHHHTK KHSHHHKHSYI        SPSPS SP
Sbjct: 1   MSSFVLSFAIWFSFISCLLSSSSQARWHHHTKQKHSHHHKHSYISHPPSLSPSPSPSDSP 60

Query: 61  LPDDGDYVGETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPYSFSFMIQ 120
            PDDGDY+ ETN+FDVRAFGAIG+GV DDT AFKMAWDTACQSDDSAIMLVPY +SFMIQ
Sbjct: 61  -PDDGDYINETNIFDVRAFGAIGNGVVDDTEAFKMAWDTACQSDDSAIMLVPYGYSFMIQ 120

Query: 121 STIFTGPCHGHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQ 180
           STIFTGPCHGH+MLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDG+GQ
Sbjct: 121 STIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQ 180

Query: 181 KWWDLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVH 240
           KWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVH
Sbjct: 181 KWWDLPCKPHKGRNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVH 240

Query: 241 IDSIHILSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSH 300
           IDSIHI SPGLSPNTDGIHIENTNGV IYNSVIYNGDDCVSIGSGSYD+DIRNITCGPSH
Sbjct: 241 IDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDLDIRNITCGPSH 300

Query: 301 GISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRN 360
           GISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRN
Sbjct: 301 GISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRN 360

Query: 361 PIIIDQYYCLTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEI 420
           PIIIDQ+YCLTKDC+NQTSAVS+SDI+Y+NIKGTYDIRSPPMHFACSDSVPCTNLTLSEI
Sbjct: 361 PIIIDQFYCLTKDCINQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEI 420

Query: 421 ELLPAQGDIVLDPFCWNAYGDLETLTIPPVSCLQEGPPSSIKLSNENEYC 462
           ELLP+QGDIVLDPFCWNAYGDL+TLTIPPVSCLQEG PSSI L+N+NEYC
Sbjct: 421 ELLPSQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 469

BLAST of Sed0004566 vs. NCBI nr
Match: XP_022992012.1 (polygalacturonase At1g48100 [Cucurbita maxima])

HSP 1 Score: 889.0 bits (2296), Expect = 1.7e-254
Identity = 411/461 (89.15%), Postives = 438/461 (95.01%), Query Frame = 0

Query: 1   MSSFVLSIAFWFSFIFCLLFSSQARWHHHTKLKHSHHHKHSYISPSPSPSPLPDDGDYVG 60
           MS+FVLS++FW +F+ CLL SSQARWHHH+K KHSHHHK SYIS  P+PSP P DGDY+ 
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60

Query: 61  ETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPYSFSFMIQSTIFTGPCH 120
           ETN+FDVR FGA+GDGVADDT AFKMAWDTACQSDD+A++LVPY +SFMIQSTIFTGPCH
Sbjct: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDDAAVVLVPYGYSFMIQSTIFTGPCH 120

Query: 121 GHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWWDLPCKP 180
           GH+ML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DG+GQKWWDLPCKP
Sbjct: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180

Query: 181 HKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHILSP 240
           HKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCKNVHI+SIHI SP
Sbjct: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240

Query: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300
           GLSPNTDGIHIENTN VGIYNS+IYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV
Sbjct: 241 GLSPNTDGIHIENTNDVGIYNSIIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300

Query: 301 RNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYC 360
           RNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPIIIDQ+YC
Sbjct: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360

Query: 361 LTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDI 420
           LTKDC NQTSAVSISDIEY+NIKGTYDIRS PMH ACSD+VPCTNLTLSEIELLPAQGDI
Sbjct: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420

Query: 421 VLDPFCWNAYGDLETLTIPPVSCLQEGPPSSIKLSNENEYC 462
           VLDPFCWNAYGDL+TLTIPPVSCLQEG PSSIKL+N+NEYC
Sbjct: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of Sed0004566 vs. NCBI nr
Match: KAG7014289.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 888.6 bits (2295), Expect = 2.2e-254
Identity = 412/461 (89.37%), Postives = 438/461 (95.01%), Query Frame = 0

Query: 1   MSSFVLSIAFWFSFIFCLLFSSQARWHHHTKLKHSHHHKHSYISPSPSPSPLPDDGDYVG 60
           MS+FVLS++FW +F+ CLL SSQARWHHH+K KHSHHHK SYIS  P+PSP P DGDY+ 
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60

Query: 61  ETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPYSFSFMIQSTIFTGPCH 120
           ETN+FDVR FGAIGDGVADDT AFKMAWD ACQSDD+A++LVPY +SFMIQSTIFTGPCH
Sbjct: 61  ETNIFDVRGFGAIGDGVADDTEAFKMAWDMACQSDDAAVVLVPYGYSFMIQSTIFTGPCH 120

Query: 121 GHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWWDLPCKP 180
           GH+ML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DG+G+KWWDLPCKP
Sbjct: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGKKWWDLPCKP 180

Query: 181 HKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHILSP 240
           HKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCKNVHI+SIHI SP
Sbjct: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240

Query: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300
           GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV
Sbjct: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300

Query: 301 RNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYC 360
           RNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPIIIDQ+YC
Sbjct: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360

Query: 361 LTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDI 420
           LTKDC NQTSAVSISDIEY+NIKGTYDIRS PMH ACSD+VPCTNLTLSEIELLPAQGDI
Sbjct: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420

Query: 421 VLDPFCWNAYGDLETLTIPPVSCLQEGPPSSIKLSNENEYC 462
           VLDPFCWNAYGDL+TLTIPPVSCLQEG PSSIKL+N+NEYC
Sbjct: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of Sed0004566 vs. NCBI nr
Match: XP_022953772.1 (polygalacturonase At1g48100 [Cucurbita moschata])

HSP 1 Score: 888.3 bits (2294), Expect = 2.9e-254
Identity = 411/461 (89.15%), Postives = 437/461 (94.79%), Query Frame = 0

Query: 1   MSSFVLSIAFWFSFIFCLLFSSQARWHHHTKLKHSHHHKHSYISPSPSPSPLPDDGDYVG 60
           MS+FVLS++FW +F+ CLL SSQARWHHH+K KHSHHHK SYIS  P+PSP P DGDY+ 
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60

Query: 61  ETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPYSFSFMIQSTIFTGPCH 120
           ETN+FDVR FGA+GDGVADDT AFKMAWD ACQSDD+A++LVPY +SFMIQSTIFTGPCH
Sbjct: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDMACQSDDAAVVLVPYGYSFMIQSTIFTGPCH 120

Query: 121 GHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWWDLPCKP 180
           GH+ML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DG+GQKWWDLPCKP
Sbjct: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180

Query: 181 HKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHILSP 240
           HKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCKNVHI+SIHI SP
Sbjct: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240

Query: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300
           G SPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV
Sbjct: 241 GFSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300

Query: 301 RNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYC 360
           RNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPIIIDQ+YC
Sbjct: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360

Query: 361 LTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDI 420
           LTKDC NQTSAVSISDIEY+NIKGTYDIRS PMH ACSD+VPCTNLTLSEIELLPAQGDI
Sbjct: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420

Query: 421 VLDPFCWNAYGDLETLTIPPVSCLQEGPPSSIKLSNENEYC 462
           VLDPFCWNAYGDL+TLTIPPVSCLQEG PSSIKL+N+NEYC
Sbjct: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of Sed0004566 vs. NCBI nr
Match: KAG6575738.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 887.9 bits (2293), Expect = 3.8e-254
Identity = 411/461 (89.15%), Postives = 438/461 (95.01%), Query Frame = 0

Query: 1   MSSFVLSIAFWFSFIFCLLFSSQARWHHHTKLKHSHHHKHSYISPSPSPSPLPDDGDYVG 60
           MS+FVLS++FW +F+ CLL SSQARWHHH+K KHSHHHK SYIS  P+PSP P DGDY+ 
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60

Query: 61  ETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPYSFSFMIQSTIFTGPCH 120
           ETN+FDVR FGA+GDGVADDT AFKMAWD ACQSD++A++LVPY +SFMIQSTIFTGPCH
Sbjct: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDMACQSDNAAVVLVPYGYSFMIQSTIFTGPCH 120

Query: 121 GHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWWDLPCKP 180
           GH+ML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DG+GQKWWDLPCKP
Sbjct: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180

Query: 181 HKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHILSP 240
           HKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCKNVHI+SIHI SP
Sbjct: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240

Query: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300
           GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV
Sbjct: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300

Query: 301 RNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYC 360
           RNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPIIIDQ+YC
Sbjct: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360

Query: 361 LTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDI 420
           LTKDC NQTSAVSISDIEY+NIKGTYDIRS PMH ACSD+VPCTNLTLSEIELLPAQGDI
Sbjct: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420

Query: 421 VLDPFCWNAYGDLETLTIPPVSCLQEGPPSSIKLSNENEYC 462
           VLDPFCWNAYGDL+TLTIPPVSCLQEG PSSIKL+N+NEYC
Sbjct: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of Sed0004566 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 1.8e-161
Identity = 257/417 (61.63%), Postives = 321/417 (76.98%), Query Frame = 0

Query: 45  PSPSPSPLPDDGDYVGETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPY 104
           P PSP P P D D      VFDV +FGA+GDG  DDT AF+ AW  AC + +S ++L P 
Sbjct: 63  PYPSPDPAPGDSD---SGCVFDVTSFGAVGDGSCDDTAAFQDAWKAAC-AVESGVVLAPE 122

Query: 105 SFSFMIQSTIFTGPCHGHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGG 164
              F I STIF+GPC   ++ ++DG LMPPDGP+ WP+  ++ QWLVFYR+DG +  G G
Sbjct: 123 GGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKG 182

Query: 165 VIDGKGQKWWDLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRF 224
            ++G GQKWWDLPCKPH+G +G++  GPC SP  IRFFMS+N+ V+GL+I NSPQFH +F
Sbjct: 183 TVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKF 242

Query: 225 DGCKNVHIDSIHILSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRN 284
           DGC+ V I+ I I SP LSPNTDGIH+ NT  VGIYNSV+ NGDDC+SIG+G  DVDI+ 
Sbjct: 243 DGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQG 302

Query: 285 ITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNI 344
           +TCGPSHGISIGSLGV NS+ACV+NITVR++VI+ SDNG+R+KTWQGG G+V  L F NI
Sbjct: 303 VTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENI 362

Query: 345 HMDNVRNPIIIDQYYCLTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCT 404
            M+NV N II+DQYYC +KDC N+TSAV + D+EY NIKGTYD+RSPP+HFACSD+V CT
Sbjct: 363 QMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACT 422

Query: 405 NLTLSEIELLPAQGDIVLDPFCWNAYGDLETLTIPPVSCLQEGPPSSIKLSNENEYC 462
           N+T+SE+ELLP +G++V DPFCWNAYG  ETLTIPP+ CL +G P   +  + N  C
Sbjct: 423 NITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

BLAST of Sed0004566 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 8.6e-92
Identity = 167/379 (44.06%), Postives = 231/379 (60.95%), Query Frame = 0

Query: 70  FGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPYSFSFMIQSTIFTGPCHGHVMLRVDG 129
           FGA G+GV DDT AF  AW TAC S     +LVP +++ +++    +GPC   + L++ G
Sbjct: 58  FGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARLTLQISG 117

Query: 130 TLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWWDLPCKPHKGLNGTTL 189
           T++ P+ PD W +  +RR+WL F+ +  +++EGGG ++G GQ+WW+  CK H   N    
Sbjct: 118 TIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK-HNHSN---- 177

Query: 190 PGPC-DSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHILSPGLSPNTDG 249
             PC  +P A+ F    N+ V+ L + +S Q H     C+ V I  + +++P  SPNTDG
Sbjct: 178 --PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDG 237

Query: 250 IHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRACVT 309
           IHI  + G+ I N+ +  GDDC+SI   S  + I NI CGP HGISIGSLG   S   V 
Sbjct: 238 IHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVR 297

Query: 310 NITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYC-LTKDCMN 369
           +ITV  ++I  + NGVRIKTWQGG G V  + F NI M+NV NPIIIDQYYC   K C N
Sbjct: 298 DITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCAN 357

Query: 370 QTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIVLDPFCW 429
           QTSA+SI +I + +++GT       +  +CSDS PC N+ L +I+L P+ GD   + FCW
Sbjct: 358 QTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCW 417

Query: 430 NAYGDLETLTIPPVSCLQE 447
            AYG       PP  CL +
Sbjct: 418 EAYGSSSGQVYPP-PCLSD 426

BLAST of Sed0004566 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 8.6e-84
Identity = 167/399 (41.85%), Postives = 230/399 (57.64%), Query Frame = 0

Query: 63  NVFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPYSFSFMIQSTIFTGPCHGH 122
           N+F+V  +GA+GDG  D T AF  AW  AC+   SA++LVP +  F++ +  F GPC  H
Sbjct: 57  NIFNVEKYGAVGDGKHDCTEAFSTAWQAACKK-PSAMLLVPGNKKFVVNNLFFNGPCQPH 116

Query: 123 VMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWWDLPCKPHK 182
              +VDG +     P +W  N   R WL F ++ G +L G GVIDG+G++WW   C   K
Sbjct: 117 FTFKVDGIIAAYQNPASWKNN---RIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQC---K 176

Query: 183 GLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHILSPGL 242
            +NG  +    D P AI+F  S+ L +QGLK+ NSP+FH  F  C+ V I  I I +P  
Sbjct: 177 WVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRD 236

Query: 243 SPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRN 302
           SPNTDGI I  +    +  + I  GDDCV+IG+GS ++ I ++ CGP HGISIGSLG  N
Sbjct: 237 SPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGREN 296

Query: 303 SRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYCLT 362
           SRA V+ + V  +    + NG+RIKTWQGG G    + + N+ M N  NPI+I+Q+YC +
Sbjct: 297 SRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTS 356

Query: 363 KD-CMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIV 422
              C NQ SAV I D+ Y NI+GT    +  +   CSDS+PC ++ LS+I L    G I 
Sbjct: 357 ASACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKIA 416

Query: 423 LDPFCWNAYGDLETLTIPPVSCLQEGPPSSIKLSNENEY 461
                 NA G      IP  +C    P +  K S  +++
Sbjct: 417 -SCLNDNANGYFSGHVIP--ACKNLSPSAKRKESKSHKH 444

BLAST of Sed0004566 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 5.2e-81
Identity = 160/384 (41.67%), Postives = 224/384 (58.33%), Query Frame = 0

Query: 66  DVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPYSFSFMIQSTIFTGPCHGHVML 125
           +V  FGA GDG  DDT AF+ AW  AC S  SA++LVP   +++++   F+GPC   + +
Sbjct: 91  NVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKK-NYLVRPISFSGPCKSGLTM 150

Query: 126 RVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWWDLPCKPHKGLN 185
           ++ GT+   D    + K+   R WLVF  +  + +EGGG I+G G+ WW   CK +K L 
Sbjct: 151 QIYGTIEASDDRSDYRKDG--RHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKAL- 210

Query: 186 GTTLPGPC-DSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHILSPGLSP 245
                 PC D+P A+ F+ S ++ V+ LKI N+ Q H  FD C NV   ++ + +P  SP
Sbjct: 211 ------PCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSP 270

Query: 246 NTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSR 305
           NTDGIH+  T  + I + VI  GDDC+SI +GS  V + +ITCGP HGISIGSLG  NS 
Sbjct: 271 NTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSE 330

Query: 306 ACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYC-LTK 365
           A V+++ V  + +  + NGVRIKTWQGG G+   + F N+ M NV NPIIIDQ YC   K
Sbjct: 331 AHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDK 390

Query: 366 DCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIVLD 425
            C  Q+SAV + ++ Y NIKGT    +  + F CS   PC  + L +++ L  +G     
Sbjct: 391 PCQEQSSAVQVKNVVYQNIKGTC-ASNVAITFDCSKRFPCQGIVLEDVD-LEIEGGAAAK 450

Query: 426 PFCWNAYGDLETLTIPPVSCLQEG 448
             C N   +L    +    C +EG
Sbjct: 451 ALCNNV--ELSETGVVSPHCQEEG 459

BLAST of Sed0004566 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 6.8e-81
Identity = 166/398 (41.71%), Postives = 231/398 (58.04%), Query Frame = 0

Query: 64  VFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPYSFSFMIQSTIFTGPCHGHV 123
           VF+V  +GA+GDG  D T AF   W+ AC+   SA++LVP +  F + + +F GPC  H+
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKK-ASAVLLVPANKKFFVNNLVFRGPCQPHL 117

Query: 124 MLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWWDLPCKPHKG 183
             +VDGT++    P  W KN+  + WL F ++   +L G GVIDG+GQ+WW   CK    
Sbjct: 118 SFKVDGTIVAQPDPARW-KNS--KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV--- 177

Query: 184 LNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHILSPGLS 243
           +NG T+    + P AI+   S ++TV+ L + NSP+FH  F  C+ V I  + I +P  S
Sbjct: 178 VNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDS 237

Query: 244 PNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNS 303
           PNTDGI I  +    I   VI  GDDC++IG+GS ++ I+++ CGP HGISIGSLG  NS
Sbjct: 238 PNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNS 297

Query: 304 RACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYCLTK 363
           RA V+++ V  +    + NG+RIKTWQGG G    +T+ N+ M N  NPI+I+Q+YC + 
Sbjct: 298 RAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSA 357

Query: 364 D-CMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGD--I 423
             C NQ SAV I  + Y NI GT    +  +   CSDSVPCT + LS + L    G    
Sbjct: 358 SACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSLKLTSGKPAS 417

Query: 424 VLDPFCWNAYGDLETLTIPPVSCLQEGP-PSSIKLSNE 458
            +D    NA G      IP    L+ GP P   +L  +
Sbjct: 418 CVDK---NARGFYSGRLIPTCKNLRPGPSPKEFELQQQ 444

BLAST of Sed0004566 vs. ExPASy TrEMBL
Match: A0A6J1JNI5 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111488489 PE=3 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 8.3e-255
Identity = 411/461 (89.15%), Postives = 438/461 (95.01%), Query Frame = 0

Query: 1   MSSFVLSIAFWFSFIFCLLFSSQARWHHHTKLKHSHHHKHSYISPSPSPSPLPDDGDYVG 60
           MS+FVLS++FW +F+ CLL SSQARWHHH+K KHSHHHK SYIS  P+PSP P DGDY+ 
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60

Query: 61  ETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPYSFSFMIQSTIFTGPCH 120
           ETN+FDVR FGA+GDGVADDT AFKMAWDTACQSDD+A++LVPY +SFMIQSTIFTGPCH
Sbjct: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDDAAVVLVPYGYSFMIQSTIFTGPCH 120

Query: 121 GHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWWDLPCKP 180
           GH+ML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DG+GQKWWDLPCKP
Sbjct: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180

Query: 181 HKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHILSP 240
           HKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCKNVHI+SIHI SP
Sbjct: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240

Query: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300
           GLSPNTDGIHIENTN VGIYNS+IYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV
Sbjct: 241 GLSPNTDGIHIENTNDVGIYNSIIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300

Query: 301 RNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYC 360
           RNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPIIIDQ+YC
Sbjct: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360

Query: 361 LTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDI 420
           LTKDC NQTSAVSISDIEY+NIKGTYDIRS PMH ACSD+VPCTNLTLSEIELLPAQGDI
Sbjct: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420

Query: 421 VLDPFCWNAYGDLETLTIPPVSCLQEGPPSSIKLSNENEYC 462
           VLDPFCWNAYGDL+TLTIPPVSCLQEG PSSIKL+N+NEYC
Sbjct: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of Sed0004566 vs. ExPASy TrEMBL
Match: A0A6J1GP58 (polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111456199 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.4e-254
Identity = 411/461 (89.15%), Postives = 437/461 (94.79%), Query Frame = 0

Query: 1   MSSFVLSIAFWFSFIFCLLFSSQARWHHHTKLKHSHHHKHSYISPSPSPSPLPDDGDYVG 60
           MS+FVLS++FW +F+ CLL SSQARWHHH+K KHSHHHK SYIS  P+PSP P DGDY+ 
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60

Query: 61  ETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPYSFSFMIQSTIFTGPCH 120
           ETN+FDVR FGA+GDGVADDT AFKMAWD ACQSDD+A++LVPY +SFMIQSTIFTGPCH
Sbjct: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDMACQSDDAAVVLVPYGYSFMIQSTIFTGPCH 120

Query: 121 GHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWWDLPCKP 180
           GH+ML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DG+GQKWWDLPCKP
Sbjct: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180

Query: 181 HKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHILSP 240
           HKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCKNVHI+SIHI SP
Sbjct: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240

Query: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300
           G SPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV
Sbjct: 241 GFSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300

Query: 301 RNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYC 360
           RNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPIIIDQ+YC
Sbjct: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360

Query: 361 LTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDI 420
           LTKDC NQTSAVSISDIEY+NIKGTYDIRS PMH ACSD+VPCTNLTLSEIELLPAQGDI
Sbjct: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420

Query: 421 VLDPFCWNAYGDLETLTIPPVSCLQEGPPSSIKLSNENEYC 462
           VLDPFCWNAYGDL+TLTIPPVSCLQEG PSSIKL+N+NEYC
Sbjct: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of Sed0004566 vs. ExPASy TrEMBL
Match: A0A6J1CXU8 (polygalacturonase At1g48100 OS=Momordica charantia OX=3673 GN=LOC111015443 PE=3 SV=1)

HSP 1 Score: 882.5 bits (2279), Expect = 7.7e-253
Identity = 415/466 (89.06%), Postives = 436/466 (93.56%), Query Frame = 0

Query: 1   MSSFVLSIAFWFSFIFCLLFSSQARWHHHTKLKHSHHHKHSYISPSPSPSP-----LPDD 60
           M+SFVLS+AFW SFI CL+ SSQ RWHH  K KHSHHHKHSYIS  PSP P      PD+
Sbjct: 1   MASFVLSLAFWCSFICCLV-SSQGRWHHPPKQKHSHHHKHSYISQPPSPPPPPPPDSPDE 60

Query: 61  GDYVGETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPYSFSFMIQSTIF 120
           GDYV E +VFDVRAFGAIGDG ADDT AFKMAWDTACQSDD A++LVPYSFSFMIQSTIF
Sbjct: 61  GDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIF 120

Query: 121 TGPCHGHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWWD 180
           TGPC GH+MLRVDGTLMPPDGPDAWPKN S RQWLVFYRIDGMSLEGGGVIDG+GQKWWD
Sbjct: 121 TGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWD 180

Query: 181 LPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSI 240
           LPCKPHKG+NGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSI
Sbjct: 181 LPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSI 240

Query: 241 HILSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISI 300
           HI SPGLSPNTDGIHIENTNGV IYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISI
Sbjct: 241 HIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISI 300

Query: 301 GSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIII 360
           GSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRG+TFSN+HMDNVRNPIII
Sbjct: 301 GSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIII 360

Query: 361 DQYYCLTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLP 420
           DQ+YCL+KDC NQTSAVS+SDI+YSNIKGTYDIRSPPMH ACSDSVPCTNLTLSEIELLP
Sbjct: 361 DQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLP 420

Query: 421 AQGDIVLDPFCWNAYGDLETLTIPPVSCLQEGPPSSIKLSNENEYC 462
           AQGDIVLDP+CWNAYGDL+TLTIPP+SCLQEG PSSI L+N+NEYC
Sbjct: 421 AQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465

BLAST of Sed0004566 vs. ExPASy TrEMBL
Match: A0A5D3BFC7 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00080 PE=3 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.2e-252
Identity = 417/467 (89.29%), Postives = 437/467 (93.58%), Query Frame = 0

Query: 1   MSSFV-LSIAFWFSFIFCLLFSSQARWHHHTKLKHSHHHKHSYISP----SPSPSPLPDD 60
           MSSFV LS A W SF+ CLL SSQARWHHHTK KHSHHH+HSYIS     SPSPS  PDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GDYVGETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQS-DDSAIMLVPYSFSFMIQSTI 120
           GDY+ +TN+FDVRAFGAIGDG+ DDT AFKMAWDTACQS DDSA+MLVPY FSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWW 180
           FTGPCHG++MLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVIDG+GQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDS 240
           DLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHILSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGIS 300
           IHI SPGLSPNTDGIHIENTNGV IYNSVI NGDDCVSIGSGSYD+DIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQYYCLTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420
           IDQ+YCLTKDC NQTSAVS+SDI+Y+NIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLETLTIPPVSCLQEGPPSSIKLSNENEYC 462
           PAQGDIVLDPFCWNAYGDL+TLTIPPVSCLQEG PSSI  +N+NEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Sed0004566 vs. ExPASy TrEMBL
Match: A0A1S3CFX8 (polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 6.5e-252
Identity = 416/467 (89.08%), Postives = 436/467 (93.36%), Query Frame = 0

Query: 1   MSSFV-LSIAFWFSFIFCLLFSSQARWHHHTKLKHSHHHKHSYISP----SPSPSPLPDD 60
           MSSFV LS A W SF+ CLL SSQARWHHHTK KHSHHH+H YIS     SPSPS  PDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHGYISHPPSLSPSPSDSPDD 60

Query: 61  GDYVGETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQS-DDSAIMLVPYSFSFMIQSTI 120
           GDY+ +TN+FDVRAFGAIGDG+ DDT AFKMAWDTACQS DDSA+MLVPY FSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWW 180
           FTGPCHG++MLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVIDG+GQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDS 240
           DLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHILSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGIS 300
           IHI SPGLSPNTDGIHIENTNGV IYNSVI NGDDCVSIGSGSYD+DIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQYYCLTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420
           IDQ+YCLTKDC NQTSAVS+SDI+Y+NIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLETLTIPPVSCLQEGPPSSIKLSNENEYC 462
           PAQGDIVLDPFCWNAYGDL+TLTIPPVSCLQEG PSSI  +N+NEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Sed0004566 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 645.6 bits (1664), Expect = 3.1e-185
Identity = 303/468 (64.74%), Postives = 370/468 (79.06%), Query Frame = 0

Query: 11  WFSFIFCLLFSSQARWHHH-TKLKHSHHHKHSY--------------------ISPSPSP 70
           + + I  L+  S+AR HHH  K KH++H+ HS                      SP+PS 
Sbjct: 14  FMTLILTLMSLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAPSL 73

Query: 71  SPLPDDGDYVGETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDS--AIMLVPYSFS 130
            P P D        +++VR +GA+GDG  DDT AFK AWD++C ++++  +++LVPY ++
Sbjct: 74  PPSPSDDPADDNNGIYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLLVPYGYT 133

Query: 131 FMIQSTIFTGPCHGHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVID 190
           FMIQSTIFTGPC  +   +VDGT++ PDGP++WP N S+RQWLVFYR++GM+L+G GVID
Sbjct: 134 FMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKGEGVID 193

Query: 191 GKGQKWWDLPCKPHKGLNGTTL-PGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDG 250
           G+GQKWWDLPCKPH+ +N + +  GPCDSPIA+RFFMSSNL V+GL+I NSPQFHFRFDG
Sbjct: 194 GRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFRFDG 253

Query: 251 CKNVHIDSIHILSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNIT 310
           C+ VH++S+HI +P LSPNTDGIHIEN+N V IYNS+I NGDDCVSIGSGSYDVDIRN+T
Sbjct: 254 CQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDVDIRNLT 313

Query: 311 CGP-SHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIH 370
           CGP  HGISIGSLG  NSRACV+NITVRDSVIK SDNGVRIKTWQGG G+V G+TF+NIH
Sbjct: 314 CGPGGHGISIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGVTFNNIH 373

Query: 371 MDNVRNPIIIDQYYCLTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTN 430
           +D+VRNPIIIDQYYC+TKDC N+TSAV +SDI Y  IKGTYDIRSPPMHF CSD+VPCTN
Sbjct: 374 VDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSDAVPCTN 433

Query: 431 LTLSEIELLPAQGDIVLDPFCWNAYGDLETLTIPPVSCLQEGPPSSIK 454
           LTLS+IELLPA+G+IVLDPFCWNAYG  E L+IPPV CL   PP  ++
Sbjct: 434 LTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGLQ 481

BLAST of Sed0004566 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 584.3 bits (1505), Expect = 8.4e-167
Identity = 273/441 (61.90%), Postives = 342/441 (77.55%), Query Frame = 0

Query: 18  LLFSSQARWHHHTKLKHSHHHKHSYISPSPS-PSPLPDDGDYVGETNVFDVRAFGAIGDG 77
           L + ++AR HH      S        SP+P+ PS  P     +    V+DVR +GA+G+G
Sbjct: 24  LSYGTEARLHHQASQPPSP-------SPNPNDPSKSPSRSQDLDHEVVYDVRKYGAVGNG 83

Query: 78  VADDTGAFKMAWDTAC---QSDDSAIMLVPYSFSFMIQSTIFTGPCHGHVMLRVDGTLMP 137
           VADDT +FK AWD+AC   +++ ++++ VPY F+FMI+STIFTGPC  +   +VDGT++P
Sbjct: 84  VADDTVSFKTAWDSACSNNKNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYFQVDGTIVP 143

Query: 138 PDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGKGQKWWDLPCKPH-KGLNGTTLPGP 197
            DGP +WP + ++RQWL FYRI+GM+L+G GVIDG+GQ WWDLPCKPH + +N T L GP
Sbjct: 144 RDGPKSWPSSLNKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPCKPHQQNVNKTKLAGP 203

Query: 198 CDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHILSPGLSPNTDGIHIE 257
           C+SP A+RFFMSSN+ V+GL I NSPQ H + DGC  VHI+S+ I+SP  SPNTDGIHIE
Sbjct: 204 CESPAALRFFMSSNVIVKGLSIKNSPQVHLKLDGCHVVHINSLRIISPPASPNTDGIHIE 263

Query: 258 NTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGP-SHGISIGSLGVRNSRACVTNIT 317
           N+N V IYNSVI NGDDCVSIG G+YD+DIRNITCGP  HGISIGSLG +NS ACV+N+T
Sbjct: 264 NSNSVEIYNSVISNGDDCVSIGPGAYDIDIRNITCGPGGHGISIGSLGEKNSHACVSNVT 323

Query: 318 VRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYCLTKDCMNQTSA 377
           VRDS IK S+NGVRIKTWQGG G+V G+TF NIH+D VRNPIIIDQYYC TK C N+TSA
Sbjct: 324 VRDSFIKFSENGVRIKTWQGGSGSVSGVTFDNIHVDTVRNPIIIDQYYCTTKSCANKTSA 383

Query: 378 VSISDIEYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIVLDPFCWNAYG 437
           V ++DI Y +IKGTYDIRSPPMHF CS++VPCTNLTLS IELLP++ DIV+ PFCWNAYG
Sbjct: 384 VFVNDIVYQSIKGTYDIRSPPMHFGCSNNVPCTNLTLSNIELLPSKEDIVVGPFCWNAYG 443

Query: 438 DLETLTIPPVSCLQEGPPSSI 453
             +  ++P +SCL+  P +S+
Sbjct: 444 ITDEFSVPLISCLKSNPSTSL 457

BLAST of Sed0004566 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 570.5 bits (1469), Expect = 1.3e-162
Identity = 257/417 (61.63%), Postives = 321/417 (76.98%), Query Frame = 0

Query: 45  PSPSPSPLPDDGDYVGETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIMLVPY 104
           P PSP P P D D      VFDV +FGA+GDG  DDT AF+ AW  AC + +S ++L P 
Sbjct: 63  PYPSPDPAPGDSD---SGCVFDVTSFGAVGDGSCDDTAAFQDAWKAAC-AVESGVVLAPE 122

Query: 105 SFSFMIQSTIFTGPCHGHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGG 164
              F I STIF+GPC   ++ ++DG LMPPDGP+ WP+  ++ QWLVFYR+DG +  G G
Sbjct: 123 GGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKG 182

Query: 165 VIDGKGQKWWDLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRF 224
            ++G GQKWWDLPCKPH+G +G++  GPC SP  IRFFMS+N+ V+GL+I NSPQFH +F
Sbjct: 183 TVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKF 242

Query: 225 DGCKNVHIDSIHILSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRN 284
           DGC+ V I+ I I SP LSPNTDGIH+ NT  VGIYNSV+ NGDDC+SIG+G  DVDI+ 
Sbjct: 243 DGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQG 302

Query: 285 ITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNI 344
           +TCGPSHGISIGSLGV NS+ACV+NITVR++VI+ SDNG+R+KTWQGG G+V  L F NI
Sbjct: 303 VTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENI 362

Query: 345 HMDNVRNPIIIDQYYCLTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSDSVPCT 404
            M+NV N II+DQYYC +KDC N+TSAV + D+EY NIKGTYD+RSPP+HFACSD+V CT
Sbjct: 363 QMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACT 422

Query: 405 NLTLSEIELLPAQGDIVLDPFCWNAYGDLETLTIPPVSCLQEGPPSSIKLSNENEYC 462
           N+T+SE+ELLP +G++V DPFCWNAYG  ETLTIPP+ CL +G P   +  + N  C
Sbjct: 423 NITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

BLAST of Sed0004566 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 570.1 bits (1468), Expect = 1.6e-162
Identity = 259/410 (63.17%), Postives = 329/410 (80.24%), Query Frame = 0

Query: 42  YISPSPSPSPLPDDGDYVGETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQS--DDSAI 101
           Y+SPSP+P+P  +D D +  T VFDV +FGAIGD   DDT AFKMAWD AC S    SA+
Sbjct: 26  YLSPSPAPNPAYNDNDNIAPT-VFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSAL 85

Query: 102 MLVPYSFSFMIQSTIFTGPCHGHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMS 161
           +LVPY+F F+++ T F GPC  +++L++DG ++ PDGP +WP N  +RQW++FYR++G+S
Sbjct: 86  LLVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSN-YQRQWMMFYRVNGLS 145

Query: 162 LEGGGVIDGKGQKWWDLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQ 221
           ++G GVI+G+GQKWW+LPCKPHKGLNGTT  GPCDSP+AIR F SS + +QG+   NS Q
Sbjct: 146 IQGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQ 205

Query: 222 FHFRFDGCKNVHIDSIHILSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYD 281
           FH RFD C +V +DS+ I +P  SPNTDGIHIENT+ V I NS+I NGDDC+SIG+G ++
Sbjct: 206 FHVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFN 265

Query: 282 VDIRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGL 341
           VDI+N+TCGPSHGISIGSLGV NS+A V+NITV +S I  SDNGVRIKTWQGG G+V  +
Sbjct: 266 VDIKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRI 325

Query: 342 TFSNIHMDNVRNPIIIDQYYCLTKDCMNQTSAVSISDIEYSNIKGTYDIRSPPMHFACSD 401
            FSNI M NVRNPI+IDQYYC T +C NQTSAV ISD+ Y+NIKGTYD+RSPP+HF CSD
Sbjct: 326 VFSNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSD 385

Query: 402 SVPCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLETLTIPPVSCLQEGPP 450
           SVPCTNLTL+E++L P++G  + +PFCWNAYG ++ +T+PPV CL + PP
Sbjct: 386 SVPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

BLAST of Sed0004566 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 379.0 bits (972), Expect = 5.3e-105
Identity = 189/445 (42.47%), Postives = 279/445 (62.70%), Query Frame = 0

Query: 42  YISPSPSPSPLPDDGDYVGETNVFDVRAFGAIGDGVADDTGAFKMAWDTACQSDDSAIML 101
           ++ P P+P PL      V ++  F+V  FGA GDG++DDT AF+ AW +AC+ + S  M+
Sbjct: 104 HVQPPPTPLPLQP----VEDSQQFNVLDFGAKGDGMSDDTQAFEAAWASACKVEAST-MI 163

Query: 102 VPYSFSFMIQSTIFTGP-CHGHVMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSL 161
           +P  + F++    F+GP C  +++ +++G ++ P   ++W        W+ F ++ G+++
Sbjct: 164 IPPDYIFLVGPISFSGPYCQANIVFQLEGMIVAPTDTESW--GGGLMWWIEFTKLSGITI 223

Query: 162 EGGGVIDGKGQKWW------DLPC----KPHKGLNGTTL---PGPCDS----------PI 221
           +G GVIDG+G  WW      D P     K    LN +     P P  S          P 
Sbjct: 224 QGNGVIDGRGTVWWQQDYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELNWRMPSIKPT 283

Query: 222 AIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHILSPGLSPNTDGIHIENTNGV 281
           A+RF+ S ++TV G+ I NSPQ H +FD C  V +  +++ SPG SPNTDGIH++NT  V
Sbjct: 284 ALRFYGSIDVTVTGITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDV 343

Query: 282 GIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVI 341
            I+ + +  GDDC+SI +G  +V + N+ CGP HGISIGSLG  +++ACV+NITVRD V+
Sbjct: 344 MIHTTTLACGDDCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACVSNITVRDVVM 403

Query: 342 KVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYCLTKDCMNQTSAVSISDI 401
             +  GVRIKTWQGG G+V+G+ FSNI +  V+ PI+IDQ+YC    CMN TSAVS+  +
Sbjct: 404 HNTMTGVRIKTWQGGIGSVKGILFSNIQLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGV 463

Query: 402 EYSNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDI-VLDPFCWNAYGDLETL 461
            Y  I+GTY ++  P+HFACSDS PC ++ LS IEL P Q    + DPFCW  +G+L + 
Sbjct: 464 TYEKIRGTYTVK--PVHFACSDSFPCIDVQLSGIELKPVQLQYHMYDPFCWKTFGELNSA 523