Sed0004528 (gene) Chayote v1

Overview
NameSed0004528
Typegene
OrganismSechium edule (Chayote v1)
DescriptiontRNA (guanine(26)-N(2))-dimethyltransferase
LocationLG03: 1652755 .. 1659975 (-)
RNA-Seq ExpressionSed0004528
SyntenySed0004528
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACAGCGAATAAAATATATCGTGCACAGAATTTTAGGGAAGTTTCAATTGTGAATCTTCAATCTCTGAAAATTCTCCCACAAAGTGTTTGGAGCTACAGAATCGGAGGCATTGGAGCTTTTTCAGGGGAGTGGGATTTTGCTTCGACCCATTGAAACCAAAAATTCTCCCACAAACTCTGTTTTTTGTTTGCATGTGAGAGCTGCAACAATGGCGACAGATCTTAGTGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCCAAGAATGAAGTCTTTTTCAATAAGACCCAGGTGTGCATTTTTACTGCAAATATGGTTTTCTGGAGTTTTGGACTTTGGGTATTGTTCTTTAGGGGAGGGAAAAAGAATCTACTTATGTTTGTTTATAGCATTTTAAGTTTGAGGGTGGTTATTGATTGAGGAATTATTTAAGATTTGGCTTGTGTTCTTATGGAAGTGAGAAAGATTGGGAGAAGAGGTATTGTTATGAAAATGGTAAACAACAACCAAAAATTTAAGAGCGAGAGACAAAGAACATACATATTTATGTTATTACTAACTTTGTCTTAGCCATGTCTGTTACAGAGGAGAGAAACCGTTTGATTTAGGAGATAAGAACACAAAAGATTTGAAAAATGAGTAAGGTTTTTAAATTTTAAGAGAGTGTATATATTGATTACTACTTGCTGACTGACTGGACAATGAGACTCCACCAATGAGTAGTCGTAGTGCAACATAGTTCAAAGCATTTCACAGGTAGCTTGGTGAAATGAATAGGCTTTGTAATTGTTCTTACTATCTTGAAACTTATCATGTTTGTGTATTTAAGTGACTGGTATTTGATGAAGAAACTAATGCATACAATTTATTCGAGTCGGGAAAGTATAGTGTGGTATGGTAAATTTCTGAAAATGAGTGGTTTTTGGATTCTCCTCTTCGATTTTTTTGCTCTACCTAGCTTTAAGCTTTTCTCATTGTAGAATCTTGACTCTTCTCCACTGTAATTCTCAGGTTAACAACAGAGACATATCCATAGCTGTCCTTAGGGCGTATATATCAATATACAAGCAGGAACATGAAGCAAAACTATCAAGAAGAGCGAAATCATATCAAAAGATTTCTGAAAACAATGGTTCTGGAATTGTCATGGAAGAAACATCTAAAGAATCTGAAACAAATGACGAAAAGAGAAATACAGGTTGTGCCATTCATGAAAAAACATCAAATGAATGTGGGGGTCCAATGTTGTCAGAAGAACGAAAAGATTTCACAGAGGGAAAAGAACAACTAGATGCAAAGCCTCTTAGAGTTCTTGAGGTTATTTTGATTTCATTGATCATTTTCTTAGGTGCATGTGCATAATTTAATTGAACTTTATTGTTCAATAAAGTGCAGTGTATGCATTTCGATTTAGGGATATGCTAATGCCTTGGTATTAGCCCCATTTTTGAGTAATATAGAGTCTATCTATTATAACCGTTATGAGTTGGTCTAGCGATCAAATAAGGGTCTTGTAAATCATAAAGTGTATAGAGGTTATAGTTCAATCTATAGTGACCACCTATCTAGGAATTAATTTCTTATGAGTTATCTTGACAACCAAATGTATAGGATTAGGTGATTGTCCAGTGAGAACAGTCAATGTGCGTATAAGTTGGGCCTGTATGCTCATAGATATATGAAAAAAAGTAATACAAAATCTATAATCTGTCTTTCCCCTACAAGGGCAACCAAATTCTGGAATGAGACAACTGGAAGGACCTTCTAGAGATGCTTAATAAGTTCGGCAACAATTTATTAATTAGCAGATGATAGACGAAGAGGCCTAAAGTTTTCACCTCTCTTATTATAATTTTATGAGCAAGACTGACTGAATTCATTAGTAGTAAATTCAAAATTGTATCTCTTATGTTAAAATTATATAGATGCATAAAAAATATATAATTTTGTTTGTGTTTGCTTCTCTTTTGGGATGTGCCTGATATTTGGTGATATGTTGTCCATTCAATACTTTATGAACAATTCTTATTTTAAATTTGAAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGAATTGGTCAAGTTGTAGCGCTAGACAATGATAAAGGTAATTTTTTATTTTGAATTATTGGTAATATAAAAAAGTTCCTCTTAACAAATATGTTTACTTGGCCATTCTTGAGGTGGTTATCAACGAAAAAGACCAACTTAAATAATGAAATTATCGGCCTTACTCTGTTAAAGGGCAGAAATATAGTATCTTGTCTGATAATTGATAATATTAGTTCCTTTAAAAATAAAAATAAAAAAATTCTCCTTTTCAGAAATGAAGTACATTGTTTATTTATTATATAATAATAATAATAATAATAATAATAATCCAAACTTTCATTGAGATGGTAAAGAAAGAACACACAAAAGGAACAAGGGCGATAATGAAGCGCTCTCTTCTACTAGGTATTTAACAATTGTAATCAGGAATGTCACCTTTAGTTTATCTACCCCCTAATATCAAAGGGAACTTTGCCTTGTACAAGCAGAGGAGGTTAGGGCCCATGCATGTGAGTGATTGTTAATGGCCAAAATGACTTGAAGAAAATGACACTTTGACATAGGTAATTTTGATCATTCTTAAATCGCTTCCAAACACGCTCTAAGTGTTAATAGTTACCCTCCTTTTATACGAATTGATTTATGTATTCATGGAAAGCATGTATTAATGTCTGAGACTGTCAGTGGATCCTTAGAGAGGGAATTTATCCATTTTACTAAGCTTTCAATAAGATATCAAGTCTTATCAATATCTATATTGATATTCCATTTAGGTGTTTCATTATAGTTTTAGTTACTAACTTACTAACTAAACTTGTTAACGATTAGATTGTTTTTTCAACAGCATCGGTTGAAGCTTGTCAGAGAAATATCAAGTTTAATGGTTCAGTTGCGTGTGCGAAAGTGGAATCACATCTTACTGATGCTCGTGTGTATATGCTCACTCACCCAAAAGAATTTGATGTGGTATGTATGCATGGGTTCCGATATGTAGGTTAGCTATTTAAAAATACATACAGGGAAGAGACTACTGAAATGCATTTTCTGAATTTCTTTTTCCTTGTTATGGTTTAGAATGGTAACGACTTAAATAATTTTATACAAAGTAATGATACGAGCACTGATGTGGATATAAGATTGAAGCTTTTATGCAGCTTATTTGAATAATTTCAGGTTGACCTAGATCCTTACGGTTCGCCTTCTGTTTTCTTGGACTCCGCTGTTCAATCAGTTGTTGATGGTGGCATGTTGATGTGCACAGCAACTGATATGGCAGTGCTTTGTGGGGGAAATGGAGAAGTTTGCTATTCAAAGTAAGGATTTTTTACAGTCCTCATTTGTCATTTTTTTAATGTTCTATTGCCATAGACTATGTGTTCTGAAAATTGCTGATCGATTCAGATATGGTTCCTATCCCTTGAGAGGAAAATATTGTCACGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGGTATACCTATCTCCTGCGCATTCCAAAAGTTTTCTCGATTAATTATTTTATCACCTTTTCTTTTCTTTTTTGGTTTAACATGCTGTACAAGATCATTCTATTTGAGTAAAAACATGCGGGATAGGGTCAAAGAGCTTACACATTGTCACATATTGCTTTGAAGAGCACAAACATGCGGGATAGATCATTCTTTATCTTTTAGTTAATTTGAGATCATTCACATAGTGCTTCACCTGCCTCTTCATTCTAGTCTCAGAACATGCAAGCCTCTTTAACTGGAATTTAATGTGCAAATTTCTGGTCTCGCTAACAAGCAGAAGGAGTTTATAAACTTCCTTAAGACCCCAACTTCTGGTGTCTTATGCATTATTTTTGTTCTAAATTCTTTGCAGAGCCATGCAAATAGATACAAGCGGTATATTGTTCCCGTGCTATCTGTCCAGATGGACTTCTATGTTCGAGTTTTTGTGCGTGTTTACACGTAAGTTGATTACTCCTTGAGTTTACTGGGTTATATATTGCACCCTTTTTTTTGTCATAAGTATTTATATGAAGATGGTTTAGTTTCCTTTGATTGTTCACACTTGGGGAAATAATTGGCTGCAGTTCTGCAAGTGCAATGAAAAGTACTCCACTTAAGCTCTCATATGTTTATCAGTGCATTGGTTGCGACTCCTTCCATCTCCAGCAGATCGGGAGAACTGTCTCCAAGGTATTTTTTTTCATTACGATCTGGTATTTTTGCTGGTTATAATCACATAAATGAATTTACTTTTATTATCCAGTGTAGTTTCTCAAGTTTCTCTTGCTTATTTATACACATCCCCTGCCCCCCTTTTCTTTTCAACAACTAGCTTTCTTTGAGTCAAATGAAAGTAAAAACAAGCAGCCCACTAGAACACAGCAGTCAGCCAAAAGTGGAGAGCACTGAAAGCAAATTACGACAATGGACCCTAACTTTTGGTAGAAAAAGGTTTTGAGCTATGAGCGGAAAGGAAGCCATTAAAATGCATCAATCCCCACACATCTTCCCAATACTTGTCTAGTCCCATGATCATTTTTCCAAATGATGTCCTTCATCTCTCGAATGCTTGGGATTTTTAACAAGATAAATTTCAAAAGCCTGTCTCACGAACTACTCATGCAAAAGGGATTACCATGGATGTGACTAATATCCTCTGCATCAAACTCACTTACACACCAAGTTTGATCGATAACAAACATAGAGGTTCTTGGCAGGAGGTCAACTTCTACGAGCTAAGAAAAGAGCTGATTTTTTCATGAAAATATCTCCTGATCTACTTGAGATCTTTCTTTAGGCTTTGGACTACAGAACCCCATTCTCACGACTGGCTTGTTTGTTTTGATGTAAAATCATACTTGCTTATAAGAGGTTGGAAAGAGCGAAATAGGAGGATTTTAGAGATTAAGTAGATGGTAATTAATAAGGTTTAGGAACATTTTTGGGGGGATTTGAACCCATGATCTATTGGTGATAAGTACATACTTCATGCCAGTTGAGCTATACTCCTATTGGCCCAAGGTTTAGGAAACATTTATTTTCAAATTTCTATGCATTTTCGCTAGTAGTATTTTATTTTCCCTTTTTCTTTTGGTTCAGGATTGAGTGTGTTAGTGTTCCTTTTTCTTTTTCCTCGCCCTATTTAGGCAGACTCGACGCTTTCTGTGAGCTTCTTTGCTCTGTATTAGGATCAATTTCTTATCATGTATATTTTTTTCAATATTACAACATTGTGTTTCTCCTCAAGGAAAAAGGAAAAGAATCTGGTTGATGTAAGCTATCAATTTTGTTTTGACACTCGTGATGAACCAATCAATATATTCTAATGCTTCTTTGCTCTTAATATTGTATATTTACTTCTGATAGGAAATTAATCCTTGTTTGAAGTAGTAATTTCCTTCATTTTGAATTTGAATAGCTTCTTGACGTTTCATCTATACTTTTGCTTTCAGAACAACAGCGTCAGGCATTTACCTGGTTTTGGTCCTTCTGTTTCTCAAGAGTGCAGTGATTGTGGGAAGAAGTTCAACATGGGTGGACCTATCTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATGCTAGCAGATGTGAAGTCTAGGAAGGACTCTTATCCTGCATATGATCGTATATCTGCTGTATTGACAACGATTTCCGAGGTGTGTTTTTATGTGTGAATCTGGCTTCATAACTCCATATCATGCAGTCAGTTTGGCTAAAAAAACATTCATTGCAGCCAAGGTTTAAAATATCGATAATTCATCCCAGACATTCTAATTCATATTGCTGTTATGAGTTGATGAGTGTATTTCCTCATGTGCAGCATATGTTAACCGACTTTTTAACTGTGCAGGAATTGATGGATGTTCCCTTATTTTTGAGTTTACACAACCTCTGTGGAACATTAAAGTGCACATCTCCGTCGGCTGTAAATTTCCGTTCTGCAGTTATTAATGCCGGATATCGCATATCGGGCACTCATGTGAATCCTTTAGGACTGAAATCAGATGCTCCCATGGATGTTATTTGGGACATAATGCGTTGCTGGGTAAGACATTTTGAATTGTACAGGTCATTACATGTACTTCGACGTGGTACTGTAAAGCTTACTTACATGTATCAAGATATCATTGATCCTTTTTCTTGAAGGTCAAAAATCATCCCGTAAAAGCTCAACCAGAAGATCAGCCAGGAAGCATCATTCTTGCCAAGAAACCGGTTCTTGAAGTAAGTTTCTTTTCGTGGTAACATTAAGTTGCAATGTTTTTGCCCCTTGTTTTTTATTGTCAAATATGTTTTTATGTCCATGGACTGTTTGGGTTACCATCTGAGATGACCTTGTACATTCAATCACATAAAGCGAATGGGCTGTAGCTGATGTACTTTTGCCAATTCTTATTTCTTGGCAGGCTAATTTTGCCAGAGCTTTGGCATCTCTTAGCAAGGCACAAGCAAAGAAAGTTGCACGCTTCCTTCCGAATCCTGAAAGACATTGGGGCCCAAAGCTGAGAGCGGGTCGTCAAATCACGAGTAAGCACGTCTCTCTACTGGGTGCAGATGCAGTAAATGGAGCTCTTAACCATGCTGACTCAGAAGAGCCTAATGCAAAACGCCCCAAGGTAGAAGAAGAGCCAGCTTCCAATCAGTAAAAGTTTTTCTAATCTCATATTTTCTAAGTTATGCAATATTTAGTTTTTGACCCCACCCCACATATGAAATGCTTGTTTGTGGTCTCTTCTGTAGAAATATTAGCTTCTTTTTTTTTTATTTTTTTTTTATTTTGAGGTAGGCCTTTTCAAAATATACCAATTTTCTTCATCTCTAGAAAGTTTAGATTTCTGTCGAGAGAGACCAAGATTTTTCCTTTCCAACTTATCTTTTTTTAGTTCAACAACAATTGAGGTTAGAGGGATTTGAACTTATGACTTCTTGATCATGGACACACTTATGTCGATTGGCTATTGCTCTTGTTAGTAAAGCAAATATTAGGTTTTGAAGCTTAGGGTTGGAACATTCAGATTTGTGTTCTTGTTTTTTCTGAGTGAAAATTCTGTCTATTATATATATACGTACAAAGATTGGTGTTTGAATTATGTAAAGGAATGTGTTTTTCAAAGGGAACTCGGTC

mRNA sequence

AAAACAGCGAATAAAATATATCGTGCACAGAATTTTAGGGAAGTTTCAATTGTGAATCTTCAATCTCTGAAAATTCTCCCACAAAGTGTTTGGAGCTACAGAATCGGAGGCATTGGAGCTTTTTCAGGGGAGTGGGATTTTGCTTCGACCCATTGAAACCAAAAATTCTCCCACAAACTCTGTTTTTTGTTTGCATGTGAGAGCTGCAACAATGGCGACAGATCTTAGTGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCCAAGAATGAAGTCTTTTTCAATAAGACCCAGGTTAACAACAGAGACATATCCATAGCTGTCCTTAGGGCGTATATATCAATATACAAGCAGGAACATGAAGCAAAACTATCAAGAAGAGCGAAATCATATCAAAAGATTTCTGAAAACAATGGTTCTGGAATTGTCATGGAAGAAACATCTAAAGAATCTGAAACAAATGACGAAAAGAGAAATACAGGTTGTGCCATTCATGAAAAAACATCAAATGAATGTGGGGGTCCAATGTTGTCAGAAGAACGAAAAGATTTCACAGAGGGAAAAGAACAACTAGATGCAAAGCCTCTTAGAGTTCTTGAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGAATTGGTCAAGTTGTAGCGCTAGACAATGATAAAGCATCGGTTGAAGCTTGTCAGAGAAATATCAAGTTTAATGGTTCAGTTGCGTGTGCGAAAGTGGAATCACATCTTACTGATGCTCGTGTGTATATGCTCACTCACCCAAAAGAATTTGATGTGGTTGACCTAGATCCTTACGGTTCGCCTTCTGTTTTCTTGGACTCCGCTGTTCAATCAGTTGTTGATGGTGGCATGTTGATGTGCACAGCAACTGATATGGCAGTGCTTTGTGGGGGAAATGGAGAAGTTTGCTATTCAAAATATGGTTCCTATCCCTTGAGAGGAAAATATTGTCACGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGAGCCATGCAAATAGATACAAGCGGTATATTGTTCCCGTGCTATCTGTCCAGATGGACTTCTATGTTCGAGTTTTTGTGCGTGTTTACACTTCTGCAAGTGCAATGAAAAGTACTCCACTTAAGCTCTCATATGTTTATCAGTGCATTGGTTGCGACTCCTTCCATCTCCAGCAGATCGGGAGAACTGTCTCCAAGAACAACAGCGTCAGGCATTTACCTGGTTTTGGTCCTTCTGTTTCTCAAGAGTGCAGTGATTGTGGGAAGAAGTTCAACATGGGTGGACCTATCTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATGCTAGCAGATGTGAAGTCTAGGAAGGACTCTTATCCTGCATATGATCGTATATCTGCTGTATTGACAACGATTTCCGAGGAATTGATGGATGTTCCCTTATTTTTGAGTTTACACAACCTCTGTGGAACATTAAAGTGCACATCTCCGTCGGCTGTAAATTTCCGTTCTGCAGTTATTAATGCCGGATATCGCATATCGGGCACTCATGTGAATCCTTTAGGACTGAAATCAGATGCTCCCATGGATGTTATTTGGGACATAATGCGTTGCTGGGTCAAAAATCATCCCGTAAAAGCTCAACCAGAAGATCAGCCAGGAAGCATCATTCTTGCCAAGAAACCGGTTCTTGAAGCTAATTTTGCCAGAGCTTTGGCATCTCTTAGCAAGGCACAAGCAAAGAAAGTTGCACGCTTCCTTCCGAATCCTGAAAGACATTGGGGCCCAAAGCTGAGAGCGGGTCGTCAAATCACGAGTAAGCACGTCTCTCTACTGGGTGCAGATGCAGTAAATGGAGCTCTTAACCATGCTGACTCAGAAGAGCCTAATGCAAAACGCCCCAAGGTAGAAGAAGAGCCAGCTTCCAATCAGTAAAAGTTTTTCTAATCTCATATTTTCTAAGTTATGCAATATTTAGTTTTTGACCCCACCCCACATATGAAATGCTTGTTTGTGGTCTCTTCTGTAGAAATATTAGCTTCTTTTTTTTTTATTTTTTTTTTATTTTGAGGTAGGCCTTTTCAAAATATACCAATTTTCTTCATCTCTAGAAAGTTTAGATTTCTGTCGAGAGAGACCAAGATTTTTCCTTTCCAACTTATCTTTTTTTAGTTCAACAACAATTGAGGTTAGAGGGATTTGAACTTATGACTTCTTGATCATGGACACACTTATGTCGATTGGCTATTGCTCTTGTTAGTAAAGCAAATATTAGGTTTTGAAGCTTAGGGTTGGAACATTCAGATTTGTGTTCTTGTTTTTTCTGAGTGAAAATTCTGTCTATTATATATATACGTACAAAGATTGGTGTTTGAATTATGTAAAGGAATGTGTTTTTCAAAGGGAACTCGGTC

Coding sequence (CDS)

ATGGCGACAGATCTTAGTGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCCAAGAATGAAGTCTTTTTCAATAAGACCCAGGTTAACAACAGAGACATATCCATAGCTGTCCTTAGGGCGTATATATCAATATACAAGCAGGAACATGAAGCAAAACTATCAAGAAGAGCGAAATCATATCAAAAGATTTCTGAAAACAATGGTTCTGGAATTGTCATGGAAGAAACATCTAAAGAATCTGAAACAAATGACGAAAAGAGAAATACAGGTTGTGCCATTCATGAAAAAACATCAAATGAATGTGGGGGTCCAATGTTGTCAGAAGAACGAAAAGATTTCACAGAGGGAAAAGAACAACTAGATGCAAAGCCTCTTAGAGTTCTTGAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGAATTGGTCAAGTTGTAGCGCTAGACAATGATAAAGCATCGGTTGAAGCTTGTCAGAGAAATATCAAGTTTAATGGTTCAGTTGCGTGTGCGAAAGTGGAATCACATCTTACTGATGCTCGTGTGTATATGCTCACTCACCCAAAAGAATTTGATGTGGTTGACCTAGATCCTTACGGTTCGCCTTCTGTTTTCTTGGACTCCGCTGTTCAATCAGTTGTTGATGGTGGCATGTTGATGTGCACAGCAACTGATATGGCAGTGCTTTGTGGGGGAAATGGAGAAGTTTGCTATTCAAAATATGGTTCCTATCCCTTGAGAGGAAAATATTGTCACGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGAGCCATGCAAATAGATACAAGCGGTATATTGTTCCCGTGCTATCTGTCCAGATGGACTTCTATGTTCGAGTTTTTGTGCGTGTTTACACTTCTGCAAGTGCAATGAAAAGTACTCCACTTAAGCTCTCATATGTTTATCAGTGCATTGGTTGCGACTCCTTCCATCTCCAGCAGATCGGGAGAACTGTCTCCAAGAACAACAGCGTCAGGCATTTACCTGGTTTTGGTCCTTCTGTTTCTCAAGAGTGCAGTGATTGTGGGAAGAAGTTCAACATGGGTGGACCTATCTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATGCTAGCAGATGTGAAGTCTAGGAAGGACTCTTATCCTGCATATGATCGTATATCTGCTGTATTGACAACGATTTCCGAGGAATTGATGGATGTTCCCTTATTTTTGAGTTTACACAACCTCTGTGGAACATTAAAGTGCACATCTCCGTCGGCTGTAAATTTCCGTTCTGCAGTTATTAATGCCGGATATCGCATATCGGGCACTCATGTGAATCCTTTAGGACTGAAATCAGATGCTCCCATGGATGTTATTTGGGACATAATGCGTTGCTGGGTCAAAAATCATCCCGTAAAAGCTCAACCAGAAGATCAGCCAGGAAGCATCATTCTTGCCAAGAAACCGGTTCTTGAAGCTAATTTTGCCAGAGCTTTGGCATCTCTTAGCAAGGCACAAGCAAAGAAAGTTGCACGCTTCCTTCCGAATCCTGAAAGACATTGGGGCCCAAAGCTGAGAGCGGGTCGTCAAATCACGAGTAAGCACGTCTCTCTACTGGGTGCAGATGCAGTAAATGGAGCTCTTAACCATGCTGACTCAGAAGAGCCTAATGCAAAACGCCCCAAGGTAGAAGAAGAGCCAGCTTCCAATCAGTAA

Protein sequence

MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSRRAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTEGKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVACAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSDCGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFLSLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ
Homology
BLAST of Sed0004528 vs. NCBI nr
Match: XP_038890035.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida])

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 535/581 (92.08%), Postives = 558/581 (96.04%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR +I+  KQEHEAKLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRLFITKRKQEHEAKLSK 60

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           RAKS QK++ENNGS  VMEE S ES TNDEK +T C  HEKTSNECG PMLSEE+K+  E
Sbjct: 61  RAKSGQKVTENNGSETVMEEASNESATNDEKCHTDCGAHEKTSNECGSPMLSEEQKEVAE 120

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
           GKEQ D KP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHPKEFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VR+YTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV+QECSD
Sbjct: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
           ITSKH+SLLGA+AVNGAL+H DSEEPNAKRPKVEEEPA NQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEEEPAPNQ 581

BLAST of Sed0004528 vs. NCBI nr
Match: XP_023545495.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1073.9 bits (2776), Expect = 4.5e-310
Identity = 535/581 (92.08%), Postives = 557/581 (95.87%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRA+IS +KQEHEAK+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKHKQEHEAKISR 60

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           RAKS QKISENNGS IVM+E S ES TNDEK NT C  HEK SNECG PML EE+K+  E
Sbjct: 61  RAKSAQKISENNGSEIVMDEASNESATNDEKCNTDCDAHEKLSNECGSPMLPEEQKENVE 120

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
           GKE+ DAKP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADV+S KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVRSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
           ITSKH+SLLGADAVNGAL+HADSEEPNAKR KVEE+PA NQ
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581

BLAST of Sed0004528 vs. NCBI nr
Match: KAG6602539.1 (putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1071.2 bits (2769), Expect = 3.1e-309
Identity = 534/581 (91.91%), Postives = 555/581 (95.52%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRA+IS  KQEHE K+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           RAKS QKISENNGS IVMEE S ES TNDEK NT C  HEK SNECG P+L EE+K+  E
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLPEEQKENVE 120

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
           GKE+ DAKP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
           ITSKH+SLLGADAVNGAL+HADSEEPNAKR KVEE+PA NQ
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581

BLAST of Sed0004528 vs. NCBI nr
Match: XP_022954530.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita moschata])

HSP 1 Score: 1069.3 bits (2764), Expect = 1.2e-308
Identity = 533/581 (91.74%), Postives = 555/581 (95.52%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRA+IS  KQEHE K+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           RAKS QKISENNGS IVMEE S ES TNDEK NT C  HEK SNECG P+LSEE+K+   
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLSEEQKENVA 120

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
           GKE+ DAKP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWV+SMLADVKS KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVSSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
           ITSKH+SLLGADAVNGAL+HADSEEPNAKR KVEE+PA NQ
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581

BLAST of Sed0004528 vs. NCBI nr
Match: XP_022990611.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita maxima])

HSP 1 Score: 1065.4 bits (2754), Expect = 1.7e-307
Identity = 532/581 (91.57%), Postives = 553/581 (95.18%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRA+IS  KQEHE K+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           RAKS QKISENNGS IVMEE S ES TNDEK NT C   EK SNECG PML E +K+  E
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAREKLSNECGSPMLPEGQKENVE 120

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
           GKE+ DAKP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
           ITSKH+SLLGA+AVNGAL+HADSEEPNAKR KVEE+PA NQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581

BLAST of Sed0004528 vs. ExPASy Swiss-Prot
Match: Q9SRU7 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g02320 PE=2 SV=3)

HSP 1 Score: 851.7 bits (2199), Expect = 5.0e-246
Identity = 429/580 (73.97%), Postives = 483/580 (83.28%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR ++S  KQEHEAK S+
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           R +   K+ E + S     E SKE   ++   N G     + ++E G   +S++    TE
Sbjct: 61  RTRPASKVIEKDAS-----EASKEETPSENGMNNG---DHEVASEDGPSSVSKDPAKTTE 120

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
                + KP +VLEALSASGLRALRYARE+EGIGQVVALDND ASVEACQRNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESH TDARV+MLTHPKEFDVVDLDPYGSPS+FLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VRVYTSASAMK+TPLKLSYVYQCIGCDSFHLQ +GR++ KNNSVR+LP  GP V Q+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAP+HD EWVTS+L  VKS KD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC TLKC SPSA  FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           P+KAQ  +QPGS+IL+K+P  E +F+R + SLSKAQAKKVARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASN 581
           ITSKHVSL+G +AVNG   H        K    E EP  N
Sbjct: 541 ITSKHVSLIGHEAVNG---HLSQHHEELKEEDEEAEPEDN 569

BLAST of Sed0004528 vs. ExPASy Swiss-Prot
Match: Q9LFU5 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g15810 PE=2 SV=3)

HSP 1 Score: 841.3 bits (2172), Expect = 6.7e-243
Identity = 427/577 (74.00%), Postives = 488/577 (84.58%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLRA+I   KQEHEA LS+
Sbjct: 1   METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 60

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           RA+S  K+ E + S    EET  E+  ++ K N     HE T+ +  GP   E  K   E
Sbjct: 61  RARSSGKVVEKDVSETSKEETPTENGDDNGKTN---GEHEVTTQD--GP--KEAAKTAYE 120

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
              + + KP RVLEALSASGLRALRYAREVEGIGQVVALDND ASVEACQRNIKFNG ++
Sbjct: 121 SARR-ELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESHLTDARV+ML+HPK+FDVVDLDPYG+PS+FLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 181 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VRVYTSASAMK+TPLKLSYVYQCIGCDSFHLQ +GR++ KNNSVR+LPG GP V Q+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAPIHDQEWV S+L  VKS KD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 420

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLH+L  TLKCTSPSA  FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 421 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           P+K Q  + PGS+IL+K+P  +A+F+R + SLSKAQAKKVARFLPNPE+HWGPK+RAGR 
Sbjct: 481 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 540

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEP 578
           ITSKHVSLLG +AVNG LN+   E  + +  + EEEP
Sbjct: 541 ITSKHVSLLGHEAVNGHLNNNHKEAGDEEEEEEEEEP 569

BLAST of Sed0004528 vs. ExPASy Swiss-Prot
Match: Q3TX08 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE=1 SV=2)

HSP 1 Score: 394.8 bits (1013), Expect = 1.7e-108
Identity = 215/533 (40.34%), Postives = 320/533 (60.04%), Query Frame = 0

Query: 11  IKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSRRAKSYQKISE 70
           + EG A+I+  + NEVF+N  Q  NRD++ AV+  +  I+       L  +    +   E
Sbjct: 58  VTEGAAKIVFPSANEVFYNPVQEFNRDLTCAVITEFARIH-------LGAKGIQIKVPGE 117

Query: 71  NNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTEGKEQLDAKPL 130
            +   I ++ + +E ET  +  N       +T+                    ++  + L
Sbjct: 118 KDSEKIAVDLSDQEEETAGKNENLAPGDWPRTA-----------------AVGEICEEGL 177

Query: 131 RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVACAKVESHLTD 190
           RVLE L+ASGLR++R+A EV G+  VVA D    +VE   RN++ NG      V+ +  D
Sbjct: 178 RVLEGLAASGLRSIRFALEVPGLQSVVANDASARAVELMHRNVELNG--VAHLVQPNQAD 237

Query: 191 ARVYMLTH---PKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEV 250
           AR+ M  H   P+ FDV+DLDPYGSP+ FLD+AVQ+V DGG+L  T TDMAVL G +GE 
Sbjct: 238 ARMLMYQHQKAPERFDVIDLDPYGSPAPFLDAAVQAVSDGGLLCVTCTDMAVLAGNSGET 297

Query: 251 CYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYTSA 310
           CYSKYG+  L+ + CHEMALRI+L  ++ HAN Y+RYIVP+LS+  DFY+RVFVRV+T  
Sbjct: 298 CYSKYGAMALKSRACHEMALRIVLHSLDLHANCYQRYIVPLLSISADFYIRVFVRVFTGQ 357

Query: 311 SAMKSTPLKLSYVYQCIGCDSFHLQQIGR-TVSKNNSVRHLPGFGPSVSQECSDCGKKFN 370
           + +KS+  K + V+QC+GC +F+LQ++G+ +      ++     GP V+ EC  CG++  
Sbjct: 358 AKVKSSASKQALVFQCVGCGAFYLQRLGKASGDPGGRIKFSAACGPPVTPECEHCGQRHQ 417

Query: 371 MGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFLSLHNLC 430
           +GGP+W+ PIHD ++V  +L  V +    +    RI  VL+ ++EEL DVPL+ +L  L 
Sbjct: 418 LGGPMWAEPIHDLDFVGRVLDAVTTNPGRFHTSMRIQGVLSVVTEELPDVPLYYTLDQLS 477

Query: 431 GTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKAQ- 490
            T+ C +P  +  RSA+++AG+R+S +H     +K+DAP + +WDIMRCW K  PVK + 
Sbjct: 478 STIHCNTPRLLQLRSALLHAGFRVSLSHACKNAVKTDAPPEALWDIMRCWEKECPVKRER 537

Query: 491 -PEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRA 538
             E  P   ILA +P L+ANF     +   ++ + + RF  NPE +WGP+ RA
Sbjct: 538 LSESSPAFRILAVEPRLKANFNIREDANPSSRQRGLKRFQANPEANWGPRPRA 564

BLAST of Sed0004528 vs. ExPASy Swiss-Prot
Match: Q9NXH9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=1 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 2.3e-105
Identity = 210/535 (39.25%), Postives = 316/535 (59.07%), Query Frame = 0

Query: 9   TIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSRRAKSYQKI 68
           T + EG A+I   + NEVF+N  Q  NRD++ AV+  +  I       +L  +    +  
Sbjct: 55  TTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARI-------QLGAKGIQIKVP 114

Query: 69  SENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTEGKEQLDAK 128
            E +   +V++ + +E E  + K +   A  ++      G +  E               
Sbjct: 115 GEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEE--------------- 174

Query: 129 PLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVACAKVESHL 188
            L VLE L+ASGLR++R+A EV G+  VVA D    +V+  +RN++ N       V+   
Sbjct: 175 GLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLND--VAHLVQPSQ 234

Query: 189 TDARVYMLTHPK---EFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNG 248
            DAR+ M  H +    FDV+DLDPYGSP+ FLD+AVQ+V +GG+L  T TDMAVL G +G
Sbjct: 235 ADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSG 294

Query: 249 EVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYT 308
           E CYSKYG+  L+ + CHEMALRI+L  ++  AN Y+R++VP+LS+  DFYVRVFVRV+T
Sbjct: 295 ETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFT 354

Query: 309 SASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVS-KNNSVRHLPGFGPSVSQECSDCGKK 368
             + +K++  K + V+QC+GC +FHLQ++G+     +   +     GP V+ EC  CG++
Sbjct: 355 GQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQR 414

Query: 369 FNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFLSLHN 428
             +GGP+W+ PIHD ++V  +L  V +    +   +RI  VL+ I+EEL DVPL+ +L  
Sbjct: 415 HQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQ 474

Query: 429 LCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKA 488
           L  T+ C +PS +  RSA+++A +R+S +H     +K+DAP   +WDIMRCW K  PVK 
Sbjct: 475 LSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKR 534

Query: 489 Q--PEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRA 538
           +   E  P   IL+ +P L+ANF     +   ++ + + RF  NPE +WGP+ RA
Sbjct: 535 ERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRA 565

BLAST of Sed0004528 vs. ExPASy Swiss-Prot
Match: Q9VK89 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster OX=7227 GN=CG6388 PE=2 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 3.7e-100
Identity = 218/540 (40.37%), Postives = 305/540 (56.48%), Query Frame = 0

Query: 10  IIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSRRAKSYQKIS 69
           +I+E  AEI+  +   VF+N  Q  NRD+SIA L  Y                   Q+++
Sbjct: 19  VIRERNAEIV--SGGNVFYNPVQEFNRDLSIAALNVY------------------RQRLT 78

Query: 70  ENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTEGKEQLDAKP 129
           +      + ++  K  E  D          EKT+     P + E    + +G        
Sbjct: 79  KERSEKALKKQRKKVKEQED----------EKTTPVPEDPPVYEAGTRYEDG-------- 138

Query: 130 LRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVACAKVESHLT 189
           LR+LEAL+A+GLR++RYA+E+ G+ Q+VA D  + +V +   NI+ N      +      
Sbjct: 139 LRILEALAATGLRSIRYAQEIAGVRQIVANDLSRQAVASINTNIRHNKVEELIEPSHSDA 198

Query: 190 DARVYMLTHP-KEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVC 249
              +Y+ T P K FD VDLDPYG P+ FLD A+Q +VDGG+L+ TATDMAVL G   E C
Sbjct: 199 MTLMYLSTQPEKRFDAVDLDPYGCPNRFLDGAMQCLVDGGLLLVTATDMAVLAGNAPEAC 258

Query: 250 YSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYTSAS 309
           Y KYGS PLR K CHEMALRILL CIESHANRY +YI P+LS+  DFY+R+FVRVY   +
Sbjct: 259 YVKYGSVPLRMKCCHEMALRILLHCIESHANRYGKYIEPLLSISADFYIRIFVRVYVGQA 318

Query: 310 AMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLP-------GFGPSVSQECSDC 369
             K +  K S++YQC GC++F LQ +G  ++K N     P         GP+V+ +C  C
Sbjct: 319 QCKLSMSKQSWIYQCTGCETFTLQPLG--ITKPNPTAGNPQQLKFGIPTGPAVNSQCEHC 378

Query: 370 GKKFNMGGPIWSAPIHDQEWVTSMLADV-KSRKDSYPAYDRISAVLTTISEELMDVPLFL 429
           G + ++GGPIWSAPIH+ E+V  +L  V ++   S     RI  VL+ + EEL DVPL+ 
Sbjct: 379 GHRHHLGGPIWSAPIHNPEFVQDLLTAVQETTLQSLGTQRRIVGVLSMVQEELQDVPLYY 438

Query: 430 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 489
           +   LC  LK      + FRSA+++AGYR+S +H +   LK++AP  V+WDI+R W K H
Sbjct: 439 TPDKLCCVLKLEIVPMLKFRSAILHAGYRVSYSHASKNSLKTNAPPAVLWDILRSWSKRH 498

Query: 490 PVKAQP--EDQPGSIILAKKPVLEANFARALASLS-KAQAKKVARFLPNPERHWGPKLRA 538
           PV  +      P + IL+K+      F       + K++   ++RF  NP  HWGP  RA
Sbjct: 499 PVNPERMIPGSPLAAILSKECTAVYEFDELHPEANPKSRKSALSRFQENPTPHWGPGTRA 518

BLAST of Sed0004528 vs. ExPASy TrEMBL
Match: A0A6J1GSN6 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456770 PE=3 SV=1)

HSP 1 Score: 1069.3 bits (2764), Expect = 5.6e-309
Identity = 533/581 (91.74%), Postives = 555/581 (95.52%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRA+IS  KQEHE K+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           RAKS QKISENNGS IVMEE S ES TNDEK NT C  HEK SNECG P+LSEE+K+   
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLSEEQKENVA 120

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
           GKE+ DAKP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWV+SMLADVKS KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVSSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
           ITSKH+SLLGADAVNGAL+HADSEEPNAKR KVEE+PA NQ
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581

BLAST of Sed0004528 vs. ExPASy TrEMBL
Match: A0A6J1JNF2 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111487439 PE=3 SV=1)

HSP 1 Score: 1065.4 bits (2754), Expect = 8.1e-308
Identity = 532/581 (91.57%), Postives = 553/581 (95.18%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRA+IS  KQEHE K+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           RAKS QKISENNGS IVMEE S ES TNDEK NT C   EK SNECG PML E +K+  E
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAREKLSNECGSPMLPEGQKENVE 120

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
           GKE+ DAKP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
           ITSKH+SLLGA+AVNGAL+HADSEEPNAKR KVEE+PA NQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581

BLAST of Sed0004528 vs. ExPASy TrEMBL
Match: A0A1S3CA23 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498546 PE=3 SV=1)

HSP 1 Score: 1059.7 bits (2739), Expect = 4.5e-306
Identity = 527/581 (90.71%), Postives = 551/581 (94.84%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR++I+  KQEHEAKLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           R+KS QK+SENNGS    EE    S TN+EK +T C  HEK SNECG PMLSEE+K+  E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
           GKEQ   KP+RVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACQRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV+QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
           ITSKH+SLLGA+AVNGAL+H DSEEPNAKRPKVEEEPA NQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEEEPAPNQ 581

BLAST of Sed0004528 vs. ExPASy TrEMBL
Match: A0A0A0KVU4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G641600 PE=3 SV=1)

HSP 1 Score: 1058.1 bits (2735), Expect = 1.3e-305
Identity = 526/581 (90.53%), Postives = 551/581 (94.84%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR++I+  KQEHEAKLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           R+KS QK+SENNGS    EE   ES TNDEK +T C  HEK SNECG PMLSEE+K+  E
Sbjct: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
           GKEQ   KP+RVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV+QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GSIILAK+PVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
           ITSKH+SLLGA+AVNGAL+H DSEEPNAKR KVEEEPA NQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 581

BLAST of Sed0004528 vs. ExPASy TrEMBL
Match: A0A5A7T7H3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002310 PE=3 SV=1)

HSP 1 Score: 1055.8 bits (2729), Expect = 6.4e-305
Identity = 525/581 (90.36%), Postives = 549/581 (94.49%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           M TDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR++I+  KQEHEAKLS+
Sbjct: 1   MTTDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           R+KS QK+SENNGS    EE    S TN+EK +T C  HEK S ECG PMLSEE+K+  E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSTECGSPMLSEEQKEIVE 120

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
           GKEQ   KP+RVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACQRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV+QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
           ITSKH+SLLGA+AVNGAL+H DSEEPNAKRPKVEEEPA NQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEEEPAPNQ 581

BLAST of Sed0004528 vs. TAIR 10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 851.7 bits (2199), Expect = 3.5e-247
Identity = 429/580 (73.97%), Postives = 483/580 (83.28%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR ++S  KQEHEAK S+
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           R +   K+ E + S     E SKE   ++   N G     + ++E G   +S++    TE
Sbjct: 61  RTRPASKVIEKDAS-----EASKEETPSENGMNNG---DHEVASEDGPSSVSKDPAKTTE 120

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
                + KP +VLEALSASGLRALRYARE+EGIGQVVALDND ASVEACQRNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESH TDARV+MLTHPKEFDVVDLDPYGSPS+FLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VRVYTSASAMK+TPLKLSYVYQCIGCDSFHLQ +GR++ KNNSVR+LP  GP V Q+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAP+HD EWVTS+L  VKS KD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC TLKC SPSA  FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           P+KAQ  +QPGS+IL+K+P  E +F+R + SLSKAQAKKVARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASN 581
           ITSKHVSL+G +AVNG   H        K    E EP  N
Sbjct: 541 ITSKHVSLIGHEAVNG---HLSQHHEELKEEDEEAEPEDN 569

BLAST of Sed0004528 vs. TAIR 10
Match: AT5G15810.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 841.3 bits (2172), Expect = 4.8e-244
Identity = 427/577 (74.00%), Postives = 488/577 (84.58%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
           M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLRA+I   KQEHEA LS+
Sbjct: 99  METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 158

Query: 61  RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
           RA+S  K+ E + S    EET  E+  ++ K N     HE T+ +  GP   E  K   E
Sbjct: 159 RARSSGKVVEKDVSETSKEETPTENGDDNGKTN---GEHEVTTQD--GP--KEAAKTAYE 218

Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
              + + KP RVLEALSASGLRALRYAREVEGIGQVVALDND ASVEACQRNIKFNG ++
Sbjct: 219 SARR-ELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 278

Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESHLTDARV+ML+HPK+FDVVDLDPYG+PS+FLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 279 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 338

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 339 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 398

Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
           VRVYTSASAMK+TPLKLSYVYQCIGCDSFHLQ +GR++ KNNSVR+LPG GP V Q+C+ 
Sbjct: 399 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 458

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAPIHDQEWV S+L  VKS KD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 459 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 518

Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLH+L  TLKCTSPSA  FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 519 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 578

Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
           P+K Q  + PGS+IL+K+P  +A+F+R + SLSKAQAKKVARFLPNPE+HWGPK+RAGR 
Sbjct: 579 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 638

Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEP 578
           ITSKHVSLLG +AVNG LN+   E  + +  + EEEP
Sbjct: 639 ITSKHVSLLGHEAVNGHLNNNHKEAGDEEEEEEEEEP 667

BLAST of Sed0004528 vs. TAIR 10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 92.4 bits (228), Expect = 1.3e-18
Identity = 87/356 (24.44%), Postives = 145/356 (40.73%), Query Frame = 0

Query: 130 LRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVACAKVESHLT 189
           LRVL+A+   G+R+LRY  E E    V+A D +  +      N+        +KVE    
Sbjct: 90  LRVLDAMCGCGIRSLRYLVEAEA-DFVMANDANDDNRRVITDNL--------SKVERGTG 149

Query: 190 DARVYMLTH-------------PKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATD 249
           D R +++TH                FD++D+D +GS S FL  A  ++  GG+L  T+TD
Sbjct: 150 DERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDSSFLRDAFNALRLGGLLYLTSTD 209

Query: 250 MAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFY 309
                G       + YG++     + +E+ LR+L+      A     ++ P+ S    ++
Sbjct: 210 GYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGAVREAALLGYHVTPLFS-YYSYH 269

Query: 310 VRVFVRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQ 369
             VF  +               +V  C  C   H  +                    +  
Sbjct: 270 GPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLRFDEL--------------GLMGC 329

Query: 370 ECSD--CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS----RKDSYPAYDRISAVLTTIS 429
            CSD        + GP+W  P+HD  +VT ML   K      + +    D++ +++   S
Sbjct: 330 PCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLLSIMIEES 389

Query: 430 EELMDVPLFLSLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM 467
           +  +  P +  +  +    K  SP      SA++  GY  S +H+ P  LK+D PM
Sbjct: 390 DPRLP-PGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDCPM 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890035.10.0e+0092.08probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida][more]
XP_023545495.14.5e-31092.08probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita pepo subsp. pe... [more]
KAG6602539.13.1e-30991.91putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyr... [more]
XP_022954530.11.2e-30891.74probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita moschata][more]
XP_022990611.11.7e-30791.57probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SRU75.0e-24673.97Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana O... [more]
Q9LFU56.7e-24374.00Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana O... [more]
Q3TX081.7e-10840.34tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE... [more]
Q9NXH92.3e-10539.25tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=... [more]
Q9VK893.7e-10040.37Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster ... [more]
Match NameE-valueIdentityDescription
A0A6J1GSN65.6e-30991.74tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JNF28.1e-30891.57tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A1S3CA234.5e-30690.71tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498... [more]
A0A0A0KVU41.3e-30590.53tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A5A7T7H36.4e-30590.36tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946... [more]
Match NameE-valueIdentityDescription
AT3G02320.13.5e-24773.97N2,N2-dimethylguanosine tRNA methyltransferase [more]
AT5G15810.14.8e-24474.00N2,N2-dimethylguanosine tRNA methyltransferase [more]
AT3G56330.11.3e-1824.44N2,N2-dimethylguanosine tRNA methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042296tRNA methyltransferase, Trm1, C-terminalGENE3D3.30.56.70coord: 417..475
e-value: 1.6E-21
score: 77.7
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 8..415
e-value: 2.3E-124
score: 416.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 557..581
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 563..581
NoneNo IPR availablePANTHERPTHR10631:SF12TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE 1-RELATEDcoord: 1..563
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 131..233
e-value: 6.35817E-4
score: 37.7947
IPR002905tRNA methyltransferase, Trm1TIGRFAMTIGR00308TIGR00308coord: 11..475
e-value: 1.8E-97
score: 325.1
IPR002905tRNA methyltransferase, Trm1PFAMPF02005TRMcoord: 12..474
e-value: 1.6E-120
score: 402.8
IPR002905tRNA methyltransferase, Trm1PANTHERPTHR10631N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASEcoord: 1..563
IPR002905tRNA methyltransferase, Trm1PROSITEPS51626SAM_MT_TRM1coord: 9..473
score: 137.59079
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 8..475

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0004528.1Sed0004528.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002940 tRNA N2-guanine methylation
biological_process GO:0008033 tRNA processing
cellular_component GO:0005634 nucleus
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004809 tRNA (guanine-N2-)-methyltransferase activity
molecular_function GO:0003723 RNA binding