Homology
BLAST of Sed0004528 vs. NCBI nr
Match:
XP_038890035.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida])
HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 535/581 (92.08%), Postives = 558/581 (96.04%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR +I+ KQEHEAKLS+
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRLFITKRKQEHEAKLSK 60
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
RAKS QK++ENNGS VMEE S ES TNDEK +T C HEKTSNECG PMLSEE+K+ E
Sbjct: 61 RAKSGQKVTENNGSETVMEEASNESATNDEKCHTDCGAHEKTSNECGSPMLSEEQKEVAE 120
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
GKEQ D KP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
CAKVESHLTDARVYMLTHPKEFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VR+YTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV+QECSD
Sbjct: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
ITSKH+SLLGA+AVNGAL+H DSEEPNAKRPKVEEEPA NQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEEEPAPNQ 581
BLAST of Sed0004528 vs. NCBI nr
Match:
XP_023545495.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1073.9 bits (2776), Expect = 4.5e-310
Identity = 535/581 (92.08%), Postives = 557/581 (95.87%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRA+IS +KQEHEAK+SR
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKHKQEHEAKISR 60
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
RAKS QKISENNGS IVM+E S ES TNDEK NT C HEK SNECG PML EE+K+ E
Sbjct: 61 RAKSAQKISENNGSEIVMDEASNESATNDEKCNTDCDAHEKLSNECGSPMLPEEQKENVE 120
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
GKE+ DAKP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
CAKVESHLTDARVYMLTHP EFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADV+S KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVRSMKDTYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
ITSKH+SLLGADAVNGAL+HADSEEPNAKR KVEE+PA NQ
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581
BLAST of Sed0004528 vs. NCBI nr
Match:
KAG6602539.1 (putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1071.2 bits (2769), Expect = 3.1e-309
Identity = 534/581 (91.91%), Postives = 555/581 (95.52%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRA+IS KQEHE K+SR
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
RAKS QKISENNGS IVMEE S ES TNDEK NT C HEK SNECG P+L EE+K+ E
Sbjct: 61 RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLPEEQKENVE 120
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
GKE+ DAKP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
CAKVESHLTDARVYMLTHP EFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
ITSKH+SLLGADAVNGAL+HADSEEPNAKR KVEE+PA NQ
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581
BLAST of Sed0004528 vs. NCBI nr
Match:
XP_022954530.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita moschata])
HSP 1 Score: 1069.3 bits (2764), Expect = 1.2e-308
Identity = 533/581 (91.74%), Postives = 555/581 (95.52%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRA+IS KQEHE K+SR
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
RAKS QKISENNGS IVMEE S ES TNDEK NT C HEK SNECG P+LSEE+K+
Sbjct: 61 RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLSEEQKENVA 120
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
GKE+ DAKP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
CAKVESHLTDARVYMLTHP EFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWV+SMLADVKS KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVSSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
ITSKH+SLLGADAVNGAL+HADSEEPNAKR KVEE+PA NQ
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581
BLAST of Sed0004528 vs. NCBI nr
Match:
XP_022990611.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita maxima])
HSP 1 Score: 1065.4 bits (2754), Expect = 1.7e-307
Identity = 532/581 (91.57%), Postives = 553/581 (95.18%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRA+IS KQEHE K+SR
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
RAKS QKISENNGS IVMEE S ES TNDEK NT C EK SNECG PML E +K+ E
Sbjct: 61 RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAREKLSNECGSPMLPEGQKENVE 120
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
GKE+ DAKP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
CAKVESHLTDARVYMLTHP EFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
ITSKH+SLLGA+AVNGAL+HADSEEPNAKR KVEE+PA NQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581
BLAST of Sed0004528 vs. ExPASy Swiss-Prot
Match:
Q9SRU7 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g02320 PE=2 SV=3)
HSP 1 Score: 851.7 bits (2199), Expect = 5.0e-246
Identity = 429/580 (73.97%), Postives = 483/580 (83.28%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR ++S KQEHEAK S+
Sbjct: 1 METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
R + K+ E + S E SKE ++ N G + ++E G +S++ TE
Sbjct: 61 RTRPASKVIEKDAS-----EASKEETPSENGMNNG---DHEVASEDGPSSVSKDPAKTTE 120
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
+ KP +VLEALSASGLRALRYARE+EGIGQVVALDND ASVEACQRNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
+KVESH TDARV+MLTHPKEFDVVDLDPYGSPS+FLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VRVYTSASAMK+TPLKLSYVYQCIGCDSFHLQ +GR++ KNNSVR+LP GP V Q+C+
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKK+NMGGPIWSAP+HD EWVTS+L VKS KD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLC TLKC SPSA FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
P+KAQ +QPGS+IL+K+P E +F+R + SLSKAQAKKVARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASN 581
ITSKHVSL+G +AVNG H K E EP N
Sbjct: 541 ITSKHVSLIGHEAVNG---HLSQHHEELKEEDEEAEPEDN 569
BLAST of Sed0004528 vs. ExPASy Swiss-Prot
Match:
Q9LFU5 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g15810 PE=2 SV=3)
HSP 1 Score: 841.3 bits (2172), Expect = 6.7e-243
Identity = 427/577 (74.00%), Postives = 488/577 (84.58%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLRA+I KQEHEA LS+
Sbjct: 1 METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 60
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
RA+S K+ E + S EET E+ ++ K N HE T+ + GP E K E
Sbjct: 61 RARSSGKVVEKDVSETSKEETPTENGDDNGKTN---GEHEVTTQD--GP--KEAAKTAYE 120
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
+ + KP RVLEALSASGLRALRYAREVEGIGQVVALDND ASVEACQRNIKFNG ++
Sbjct: 121 SARR-ELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
+KVESHLTDARV+ML+HPK+FDVVDLDPYG+PS+FLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 181 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VRVYTSASAMK+TPLKLSYVYQCIGCDSFHLQ +GR++ KNNSVR+LPG GP V Q+C+
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKK+NMGGPIWSAPIHDQEWV S+L VKS KD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 420
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLH+L TLKCTSPSA FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 421 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 480
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
P+K Q + PGS+IL+K+P +A+F+R + SLSKAQAKKVARFLPNPE+HWGPK+RAGR
Sbjct: 481 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 540
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEP 578
ITSKHVSLLG +AVNG LN+ E + + + EEEP
Sbjct: 541 ITSKHVSLLGHEAVNGHLNNNHKEAGDEEEEEEEEEP 569
BLAST of Sed0004528 vs. ExPASy Swiss-Prot
Match:
Q3TX08 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE=1 SV=2)
HSP 1 Score: 394.8 bits (1013), Expect = 1.7e-108
Identity = 215/533 (40.34%), Postives = 320/533 (60.04%), Query Frame = 0
Query: 11 IKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSRRAKSYQKISE 70
+ EG A+I+ + NEVF+N Q NRD++ AV+ + I+ L + + E
Sbjct: 58 VTEGAAKIVFPSANEVFYNPVQEFNRDLTCAVITEFARIH-------LGAKGIQIKVPGE 117
Query: 71 NNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTEGKEQLDAKPL 130
+ I ++ + +E ET + N +T+ ++ + L
Sbjct: 118 KDSEKIAVDLSDQEEETAGKNENLAPGDWPRTA-----------------AVGEICEEGL 177
Query: 131 RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVACAKVESHLTD 190
RVLE L+ASGLR++R+A EV G+ VVA D +VE RN++ NG V+ + D
Sbjct: 178 RVLEGLAASGLRSIRFALEVPGLQSVVANDASARAVELMHRNVELNG--VAHLVQPNQAD 237
Query: 191 ARVYMLTH---PKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEV 250
AR+ M H P+ FDV+DLDPYGSP+ FLD+AVQ+V DGG+L T TDMAVL G +GE
Sbjct: 238 ARMLMYQHQKAPERFDVIDLDPYGSPAPFLDAAVQAVSDGGLLCVTCTDMAVLAGNSGET 297
Query: 251 CYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYTSA 310
CYSKYG+ L+ + CHEMALRI+L ++ HAN Y+RYIVP+LS+ DFY+RVFVRV+T
Sbjct: 298 CYSKYGAMALKSRACHEMALRIVLHSLDLHANCYQRYIVPLLSISADFYIRVFVRVFTGQ 357
Query: 311 SAMKSTPLKLSYVYQCIGCDSFHLQQIGR-TVSKNNSVRHLPGFGPSVSQECSDCGKKFN 370
+ +KS+ K + V+QC+GC +F+LQ++G+ + ++ GP V+ EC CG++
Sbjct: 358 AKVKSSASKQALVFQCVGCGAFYLQRLGKASGDPGGRIKFSAACGPPVTPECEHCGQRHQ 417
Query: 371 MGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFLSLHNLC 430
+GGP+W+ PIHD ++V +L V + + RI VL+ ++EEL DVPL+ +L L
Sbjct: 418 LGGPMWAEPIHDLDFVGRVLDAVTTNPGRFHTSMRIQGVLSVVTEELPDVPLYYTLDQLS 477
Query: 431 GTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKAQ- 490
T+ C +P + RSA+++AG+R+S +H +K+DAP + +WDIMRCW K PVK +
Sbjct: 478 STIHCNTPRLLQLRSALLHAGFRVSLSHACKNAVKTDAPPEALWDIMRCWEKECPVKRER 537
Query: 491 -PEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRA 538
E P ILA +P L+ANF + ++ + + RF NPE +WGP+ RA
Sbjct: 538 LSESSPAFRILAVEPRLKANFNIREDANPSSRQRGLKRFQANPEANWGPRPRA 564
BLAST of Sed0004528 vs. ExPASy Swiss-Prot
Match:
Q9NXH9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=1 SV=1)
HSP 1 Score: 384.4 bits (986), Expect = 2.3e-105
Identity = 210/535 (39.25%), Postives = 316/535 (59.07%), Query Frame = 0
Query: 9 TIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSRRAKSYQKI 68
T + EG A+I + NEVF+N Q NRD++ AV+ + I +L + +
Sbjct: 55 TTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARI-------QLGAKGIQIKVP 114
Query: 69 SENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTEGKEQLDAK 128
E + +V++ + +E E + K + A ++ G + E
Sbjct: 115 GEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEE--------------- 174
Query: 129 PLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVACAKVESHL 188
L VLE L+ASGLR++R+A EV G+ VVA D +V+ +RN++ N V+
Sbjct: 175 GLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLND--VAHLVQPSQ 234
Query: 189 TDARVYMLTHPK---EFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNG 248
DAR+ M H + FDV+DLDPYGSP+ FLD+AVQ+V +GG+L T TDMAVL G +G
Sbjct: 235 ADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSG 294
Query: 249 EVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYT 308
E CYSKYG+ L+ + CHEMALRI+L ++ AN Y+R++VP+LS+ DFYVRVFVRV+T
Sbjct: 295 ETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFT 354
Query: 309 SASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVS-KNNSVRHLPGFGPSVSQECSDCGKK 368
+ +K++ K + V+QC+GC +FHLQ++G+ + + GP V+ EC CG++
Sbjct: 355 GQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQR 414
Query: 369 FNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFLSLHN 428
+GGP+W+ PIHD ++V +L V + + +RI VL+ I+EEL DVPL+ +L
Sbjct: 415 HQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQ 474
Query: 429 LCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKA 488
L T+ C +PS + RSA+++A +R+S +H +K+DAP +WDIMRCW K PVK
Sbjct: 475 LSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKR 534
Query: 489 Q--PEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRA 538
+ E P IL+ +P L+ANF + ++ + + RF NPE +WGP+ RA
Sbjct: 535 ERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRA 565
BLAST of Sed0004528 vs. ExPASy Swiss-Prot
Match:
Q9VK89 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster OX=7227 GN=CG6388 PE=2 SV=1)
HSP 1 Score: 367.1 bits (941), Expect = 3.7e-100
Identity = 218/540 (40.37%), Postives = 305/540 (56.48%), Query Frame = 0
Query: 10 IIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSRRAKSYQKIS 69
+I+E AEI+ + VF+N Q NRD+SIA L Y Q+++
Sbjct: 19 VIRERNAEIV--SGGNVFYNPVQEFNRDLSIAALNVY------------------RQRLT 78
Query: 70 ENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTEGKEQLDAKP 129
+ + ++ K E D EKT+ P + E + +G
Sbjct: 79 KERSEKALKKQRKKVKEQED----------EKTTPVPEDPPVYEAGTRYEDG-------- 138
Query: 130 LRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVACAKVESHLT 189
LR+LEAL+A+GLR++RYA+E+ G+ Q+VA D + +V + NI+ N +
Sbjct: 139 LRILEALAATGLRSIRYAQEIAGVRQIVANDLSRQAVASINTNIRHNKVEELIEPSHSDA 198
Query: 190 DARVYMLTHP-KEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVC 249
+Y+ T P K FD VDLDPYG P+ FLD A+Q +VDGG+L+ TATDMAVL G E C
Sbjct: 199 MTLMYLSTQPEKRFDAVDLDPYGCPNRFLDGAMQCLVDGGLLLVTATDMAVLAGNAPEAC 258
Query: 250 YSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYTSAS 309
Y KYGS PLR K CHEMALRILL CIESHANRY +YI P+LS+ DFY+R+FVRVY +
Sbjct: 259 YVKYGSVPLRMKCCHEMALRILLHCIESHANRYGKYIEPLLSISADFYIRIFVRVYVGQA 318
Query: 310 AMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLP-------GFGPSVSQECSDC 369
K + K S++YQC GC++F LQ +G ++K N P GP+V+ +C C
Sbjct: 319 QCKLSMSKQSWIYQCTGCETFTLQPLG--ITKPNPTAGNPQQLKFGIPTGPAVNSQCEHC 378
Query: 370 GKKFNMGGPIWSAPIHDQEWVTSMLADV-KSRKDSYPAYDRISAVLTTISEELMDVPLFL 429
G + ++GGPIWSAPIH+ E+V +L V ++ S RI VL+ + EEL DVPL+
Sbjct: 379 GHRHHLGGPIWSAPIHNPEFVQDLLTAVQETTLQSLGTQRRIVGVLSMVQEELQDVPLYY 438
Query: 430 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 489
+ LC LK + FRSA+++AGYR+S +H + LK++AP V+WDI+R W K H
Sbjct: 439 TPDKLCCVLKLEIVPMLKFRSAILHAGYRVSYSHASKNSLKTNAPPAVLWDILRSWSKRH 498
Query: 490 PVKAQP--EDQPGSIILAKKPVLEANFARALASLS-KAQAKKVARFLPNPERHWGPKLRA 538
PV + P + IL+K+ F + K++ ++RF NP HWGP RA
Sbjct: 499 PVNPERMIPGSPLAAILSKECTAVYEFDELHPEANPKSRKSALSRFQENPTPHWGPGTRA 518
BLAST of Sed0004528 vs. ExPASy TrEMBL
Match:
A0A6J1GSN6 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456770 PE=3 SV=1)
HSP 1 Score: 1069.3 bits (2764), Expect = 5.6e-309
Identity = 533/581 (91.74%), Postives = 555/581 (95.52%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRA+IS KQEHE K+SR
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
RAKS QKISENNGS IVMEE S ES TNDEK NT C HEK SNECG P+LSEE+K+
Sbjct: 61 RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLSEEQKENVA 120
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
GKE+ DAKP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
CAKVESHLTDARVYMLTHP EFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWV+SMLADVKS KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVSSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
ITSKH+SLLGADAVNGAL+HADSEEPNAKR KVEE+PA NQ
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581
BLAST of Sed0004528 vs. ExPASy TrEMBL
Match:
A0A6J1JNF2 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111487439 PE=3 SV=1)
HSP 1 Score: 1065.4 bits (2754), Expect = 8.1e-308
Identity = 532/581 (91.57%), Postives = 553/581 (95.18%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRA+IS KQEHE K+SR
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
RAKS QKISENNGS IVMEE S ES TNDEK NT C EK SNECG PML E +K+ E
Sbjct: 61 RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAREKLSNECGSPMLPEGQKENVE 120
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
GKE+ DAKP+RVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEAC+RNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
CAKVESHLTDARVYMLTHP EFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKKVARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
ITSKH+SLLGA+AVNGAL+HADSEEPNAKR KVEE+PA NQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRSKVEEDPAPNQ 581
BLAST of Sed0004528 vs. ExPASy TrEMBL
Match:
A0A1S3CA23 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498546 PE=3 SV=1)
HSP 1 Score: 1059.7 bits (2739), Expect = 4.5e-306
Identity = 527/581 (90.71%), Postives = 551/581 (94.84%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR++I+ KQEHEAKLS+
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
R+KS QK+SENNGS EE S TN+EK +T C HEK SNECG PMLSEE+K+ E
Sbjct: 61 RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSNECGSPMLSEEQKEIVE 120
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
GKEQ KP+RVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACQRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
C KVESHLTDARVYMLTHPKEFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV+QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
ITSKH+SLLGA+AVNGAL+H DSEEPNAKRPKVEEEPA NQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEEEPAPNQ 581
BLAST of Sed0004528 vs. ExPASy TrEMBL
Match:
A0A0A0KVU4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G641600 PE=3 SV=1)
HSP 1 Score: 1058.1 bits (2735), Expect = 1.3e-305
Identity = 526/581 (90.53%), Postives = 551/581 (94.84%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR++I+ KQEHEAKLS+
Sbjct: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
R+KS QK+SENNGS EE ES TNDEK +T C HEK SNECG PMLSEE+K+ E
Sbjct: 61 RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
GKEQ KP+RVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
C+KVESHLTDARVYMLTHPKEFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV+QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVK QPEDQ GSIILAK+PVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
ITSKH+SLLGA+AVNGAL+H DSEEPNAKR KVEEEPA NQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKVEEEPAPNQ 581
BLAST of Sed0004528 vs. ExPASy TrEMBL
Match:
A0A5A7T7H3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002310 PE=3 SV=1)
HSP 1 Score: 1055.8 bits (2729), Expect = 6.4e-305
Identity = 525/581 (90.36%), Postives = 549/581 (94.49%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
M TDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR++I+ KQEHEAKLS+
Sbjct: 1 MTTDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
R+KS QK+SENNGS EE S TN+EK +T C HEK S ECG PMLSEE+K+ E
Sbjct: 61 RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSTECGSPMLSEEQKEIVE 120
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
GKEQ KP+RVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACQRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
C KVESHLTDARVYMLTHPKEFD+VDLDPYGSPS+FLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VRVYTSASAMK+TPLKLSYVYQC GCDSFHLQQIGR+VSKNNSVR+LPGFGPSV+QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLC TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
PVKAQPEDQPGSIILAK+PVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASNQ 582
ITSKH+SLLGA+AVNGAL+H DSEEPNAKRPKVEEEPA NQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKVEEEPAPNQ 581
BLAST of Sed0004528 vs. TAIR 10
Match:
AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )
HSP 1 Score: 851.7 bits (2199), Expect = 3.5e-247
Identity = 429/580 (73.97%), Postives = 483/580 (83.28%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR ++S KQEHEAK S+
Sbjct: 1 METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
R + K+ E + S E SKE ++ N G + ++E G +S++ TE
Sbjct: 61 RTRPASKVIEKDAS-----EASKEETPSENGMNNG---DHEVASEDGPSSVSKDPAKTTE 120
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
+ KP +VLEALSASGLRALRYARE+EGIGQVVALDND ASVEACQRNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
+KVESH TDARV+MLTHPKEFDVVDLDPYGSPS+FLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VRVYTSASAMK+TPLKLSYVYQCIGCDSFHLQ +GR++ KNNSVR+LP GP V Q+C+
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKK+NMGGPIWSAP+HD EWVTS+L VKS KD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLHNLC TLKC SPSA FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
P+KAQ +QPGS+IL+K+P E +F+R + SLSKAQAKKVARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEPASN 581
ITSKHVSL+G +AVNG H K E EP N
Sbjct: 541 ITSKHVSLIGHEAVNG---HLSQHHEELKEEDEEAEPEDN 569
BLAST of Sed0004528 vs. TAIR 10
Match:
AT5G15810.1 (N2,N2-dimethylguanosine tRNA methyltransferase )
HSP 1 Score: 841.3 bits (2172), Expect = 4.8e-244
Identity = 427/577 (74.00%), Postives = 488/577 (84.58%), Query Frame = 0
Query: 1 MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAYISIYKQEHEAKLSR 60
M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLRA+I KQEHEA LS+
Sbjct: 99 METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 158
Query: 61 RAKSYQKISENNGSGIVMEETSKESETNDEKRNTGCAIHEKTSNECGGPMLSEERKDFTE 120
RA+S K+ E + S EET E+ ++ K N HE T+ + GP E K E
Sbjct: 159 RARSSGKVVEKDVSETSKEETPTENGDDNGKTN---GEHEVTTQD--GP--KEAAKTAYE 218
Query: 121 GKEQLDAKPLRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVA 180
+ + KP RVLEALSASGLRALRYAREVEGIGQVVALDND ASVEACQRNIKFNG ++
Sbjct: 219 SARR-ELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 278
Query: 181 CAKVESHLTDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVL 240
+KVESHLTDARV+ML+HPK+FDVVDLDPYG+PS+FLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 279 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 338
Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 339 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 398
Query: 301 VRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQECSD 360
VRVYTSASAMK+TPLKLSYVYQCIGCDSFHLQ +GR++ KNNSVR+LPG GP V Q+C+
Sbjct: 399 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 458
Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
CGKK+NMGGPIWSAPIHDQEWV S+L VKS KD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 459 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 518
Query: 421 SLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
SLH+L TLKCTSPSA FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 519 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 578
Query: 481 PVKAQPEDQPGSIILAKKPVLEANFARALASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540
P+K Q + PGS+IL+K+P +A+F+R + SLSKAQAKKVARFLPNPE+HWGPK+RAGR
Sbjct: 579 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 638
Query: 541 ITSKHVSLLGADAVNGALNHADSEEPNAKRPKVEEEP 578
ITSKHVSLLG +AVNG LN+ E + + + EEEP
Sbjct: 639 ITSKHVSLLGHEAVNGHLNNNHKEAGDEEEEEEEEEP 667
BLAST of Sed0004528 vs. TAIR 10
Match:
AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )
HSP 1 Score: 92.4 bits (228), Expect = 1.3e-18
Identity = 87/356 (24.44%), Postives = 145/356 (40.73%), Query Frame = 0
Query: 130 LRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACQRNIKFNGSVACAKVESHLT 189
LRVL+A+ G+R+LRY E E V+A D + + N+ +KVE
Sbjct: 90 LRVLDAMCGCGIRSLRYLVEAEA-DFVMANDANDDNRRVITDNL--------SKVERGTG 149
Query: 190 DARVYMLTH-------------PKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATD 249
D R +++TH FD++D+D +GS S FL A ++ GG+L T+TD
Sbjct: 150 DERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDSSFLRDAFNALRLGGLLYLTSTD 209
Query: 250 MAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFY 309
G + YG++ + +E+ LR+L+ A ++ P+ S ++
Sbjct: 210 GYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGAVREAALLGYHVTPLFS-YYSYH 269
Query: 310 VRVFVRVYTSASAMKSTPLKLSYVYQCIGCDSFHLQQIGRTVSKNNSVRHLPGFGPSVSQ 369
VF + +V C C H + +
Sbjct: 270 GPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLRFDEL--------------GLMGC 329
Query: 370 ECSD--CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS----RKDSYPAYDRISAVLTTIS 429
CSD + GP+W P+HD +VT ML K + + D++ +++ S
Sbjct: 330 PCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLLSIMIEES 389
Query: 430 EELMDVPLFLSLHNLCGTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM 467
+ + P + + + K SP SA++ GY S +H+ P LK+D PM
Sbjct: 390 DPRLP-PGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDCPM 420
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038890035.1 | 0.0e+00 | 92.08 | probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida] | [more] |
XP_023545495.1 | 4.5e-310 | 92.08 | probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita pepo subsp. pe... | [more] |
KAG6602539.1 | 3.1e-309 | 91.91 | putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyr... | [more] |
XP_022954530.1 | 1.2e-308 | 91.74 | probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita moschata] | [more] |
XP_022990611.1 | 1.7e-307 | 91.57 | probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
Q9SRU7 | 5.0e-246 | 73.97 | Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana O... | [more] |
Q9LFU5 | 6.7e-243 | 74.00 | Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana O... | [more] |
Q3TX08 | 1.7e-108 | 40.34 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE... | [more] |
Q9NXH9 | 2.3e-105 | 39.25 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=... | [more] |
Q9VK89 | 3.7e-100 | 40.37 | Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1GSN6 | 5.6e-309 | 91.74 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... | [more] |
A0A6J1JNF2 | 8.1e-308 | 91.57 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11... | [more] |
A0A1S3CA23 | 4.5e-306 | 90.71 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498... | [more] |
A0A0A0KVU4 | 1.3e-305 | 90.53 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... | [more] |
A0A5A7T7H3 | 6.4e-305 | 90.36 | tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946... | [more] |
Match Name | E-value | Identity | Description | |
AT3G02320.1 | 3.5e-247 | 73.97 | N2,N2-dimethylguanosine tRNA methyltransferase | [more] |
AT5G15810.1 | 4.8e-244 | 74.00 | N2,N2-dimethylguanosine tRNA methyltransferase | [more] |
AT3G56330.1 | 1.3e-18 | 24.44 | N2,N2-dimethylguanosine tRNA methyltransferase | [more] |