Sed0004515 (gene) Chayote v1

Overview
NameSed0004515
Typegene
OrganismSechium edule (Chayote v1)
DescriptiontRNA (Guanine-N(7)-)-methyltransferase
LocationLG12: 1566852 .. 1570017 (+)
RNA-Seq ExpressionSed0004515
SyntenySed0004515
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCGTCACTTTGCGTCCAATTACAAACTTGCTCGCAGCTCCGTTGCATTGCTGCTGCAACGGCGGCGGCGGCGGTGGCCCCGTCGTCGAGGGTCGCCACTGCAAGCCGAGCCTTATCCCCGCTCCTTAAACGACCATTTCTTCGAAACTTCTGTTCACCATCCGCCTCTTCCGCCGACCTTTGCGATCGACAACAACTCCGGAGTCCGGAGCTGGTGGCGTTGGAATATGCAGACCTTAATTTCTCTCCCAAGATCTTTCAGGTTTGTTCTCTGTTCATAAATTTCACCTCTAATTCCTGTAACGTTGGTGAGTAATTGAAAATACGCATTTGACTTAGGGTTTTGGAATTGCAGGATTCGGGTCACGTAAGAATCAGGCAGCATGTTAATCCTCTTAGTTCTTCTTTCTCTGTATGTGATTTGAATTTTTGTGTAATTTTGTAAGAAACTACGGCTTTGAAATTTTAGGTTTGTGTTGCTTAATTGAAGGAAGGGCTCTGCCTCTTTTGCCTTTCAGGTCCCTACACCAGCACCCAATTGGGATGAAGTTTTCAAGGATCCCACATTGCCTCTTATGGTTGATATAGGAAGTGGTGAGTTGAGTTGAATCATTCCCTATTATATTACCTCTTTATATATCTGTAATTTCCATATTGCTACTTGTTTAGTGTCCGAGCAAGTCTATGCGTATTTTAACTTTAGGAGATAAATTTTGGTTCCCTATCACATTGGGATGAACCCATAATCCTGAATGTTGGTTCTTCTATGGTGATTATTATTGTCAGTCTTCTATTCATTATCCTCCTGCTTTGGTTGATATAGGAAGTGGCGAGTTGAGTTGAATTTTCCCCTATTATATGCCTCTTTACATACCTGTGTTCTCCATAATTGCTACGTGTTTCGTGTTCGAGCTAGATTATGGGTACTTCAATTTTATGAGATAAATGTTGGTTCTCCTATGGTGATTATGAATGTCAGTCTCTTATTCATTATCCTCTTGCTCTGTCTAAAAAGAGGACAGTCATGAACGAATCTTTAGAAGACATTTATTTAGGATCCTTCATTTTCTTATTCTTGGTTTGATGATGTTCGTTGAACCAGCATTGATTAGAAGGTATACTAACTGAGTAAACAAAAGGGTAGTTTGGCAAGTGAAAAAACTGTATCGAACTACTTGAAAAGGAAATATTATCCTCAAGCAACATACTGATTACTGCATGTAGTGATCTTTATTTTGCGCTATAACTCAAATTTCAATTTGATATGCATAATAAAGCAGCTCTAGTTATAAAATCAATATGGTATCTGTGGAGCAAAGTTGCTATTAAACAACAACAGCTATGTATGACTGTGAGCTATCCACCATGATTTGTGTACTTTGGGGCTAGGTAGCAGTGGTTAAGTTAAACAATGTGAATGAGGACTCGGAAAATGTTCAGAGGAAGAATACTATTGATAAAGATATTATTTCAAATGCTATCCCTGTACACATGTTAGAAAATTATTTTTCTGATACGCGTATTGATCTACGTTGCATTGTTACTTAGTGATGTTAATAAAATAGGTAAAATCCTTGATTATCCCCCAACATTGGTGCAGTCTCATTACATTCTTTTTGTGGAATTTATGAGGCTGTTTTATTCTTTTTTTGCCCATCAAGAATCTAACAGAACAGGTATTCATATTACCAGGTAGTGGCAGATTTCTCTTGTGGCTTGCAAAAAAACATCTTGATTCAAGAAATTTTTTGGGTCTCGAAATACGACAGAAAGTATGGGACCAAAAACTCACTTGTGACACACACGTTACTTGGTTTGAACCATTTGATCCTAATTCCATCGGAGTCTTATATAGTTTATTGGTTCCTTTTGTGCTCTTGCAGCTGGTCAAGCGTGCTGATGTCTGGGTCAAAGAGTTGGGATTTAGCAACGTGTAAGGATCTTAACTTCATATCAGGTAGTGAAAAAACAAGACCATCGTTTTGCAATTGATGAATTAGTAATGAACTTCTTCTTAAACTTGAATGCAGACACTTCATGTTTGCAAATGCTACAATTTCCTTTGAACAGATTATATCTTCATATCCTGGACCCCTGATTCTGGTTTCCATACTGGTTAGTGCAACAATTCATAGACTCTATATTGCCATTTTTTTCAGTCCCCCCATATAAATGAATAAAATTCAACCCGAGCTTGCTTGTTGTTTCGATTCGATTAATATCGACAATGGTAGAACTCACTCGTGTTTAATCTCATCTCAGTGCCCAGATCCTCATTTCAAGAAACGGCATCATAAGCGAAGGGTTGTACAGAAGCCTTTAATAGAATCTATTTTAGATAGTTTAATGCCTGGAGGGCAGGTATATCATCTGGTTATCCATTGCATTAAAATTTTTTGGTTGAATTACAAGTTGTTTTTGTGAATTTTAGAAAAAACATCTAAACCATCATGGAGTGCCCAATGATTAAAAGGGTCTTGGTAAGTTACGAAATGATTGGTTTAATCTATGATGGTCACCTACTTAGAAATGAATTTTATACTGATTTTTTCGACATCTAAATGCTATAAGGTTAGATGGTATGACTAATTATTATTGACGTACGCACAAATTAGCTCGGACAGTCATTAATTGAAAAGAAAAAAAAAACATTGTATGCATCAAAGACTTATTAAATATAAAGCTAAATACAAGACTTATTAGATACAAATTAAAATGTCAAAGAACTTATTAGAAGGCTTACAAAATTCTAGAATTTACTAGACAAAAATGGAAAGTTTTAAGAATGTATTAGATATTTTTGGAGTTTCTAAATCTAGATTGTTTTTTTTTTTTTTTGCCTACCCTTTCTGCGTGTGTTGTTTCAATTTGATGCTGTTTTCTCTTTAGAACATTGCTATCTGATGATGGAAATTTTGTTCTATATGATTCGACAGATTTTCATACAATCTGATGTGCTTGAAGTGGCAGTTGACATGAGACTTTGGTTCGATACTGAAACCGATATTCTCAAACACATCGACGTGCTCGACCCGAGTGCTTTGTGTGATGAAGATGGATGGCTGTTGAAAAATCCAATGGGAATAAGGACTGAAAGAGAAGTTCACGCGGAATCAGAGGGTGCGAAAATTTATAGAAGAATGTATCAAAAGTTTGCATAA

mRNA sequence

ATGGCTTCGTCACTTTGCGTCCAATTACAAACTTGCTCGCAGCTCCGTTGCATTGCTGCTGCAACGGCGGCGGCGGCGGTGGCCCCGTCGTCGAGGGTCGCCACTGCAAGCCGAGCCTTATCCCCGCTCCTTAAACGACCATTTCTTCGAAACTTCTGTTCACCATCCGCCTCTTCCGCCGACCTTTGCGATCGACAACAACTCCGGAGTCCGGAGCTGGTGGCGTTGGAATATGCAGACCTTAATTTCTCTCCCAAGATCTTTCAGGATTCGGGTCACGTAAGAATCAGGCAGCATGTTAATCCTCTTAGTTCTTCTTTCTCTGTCCCTACACCAGCACCCAATTGGGATGAAGTTTTCAAGGATCCCACATTGCCTCTTATGGTTGATATAGGAAGTGGTAGTGGCAGATTTCTCTTGTGGCTTGCAAAAAAACATCTTGATTCAAGAAATTTTTTGGGTCTCGAAATACGACAGAAACTGGTCAAGCGTGCTGATGTCTGGGTCAAAGAGTTGGGATTTAGCAACGTACACTTCATGTTTGCAAATGCTACAATTTCCTTTGAACAGATTATATCTTCATATCCTGGACCCCTGATTCTGGTTTCCATACTGTGCCCAGATCCTCATTTCAAGAAACGGCATCATAAGCGAAGGGTTGTACAGAAGCCTTTAATAGAATCTATTTTAGATAGTTTAATGCCTGGAGGGCAGATTTTCATACAATCTGATGTGCTTGAAGTGGCAGTTGACATGAGACTTTGGTTCGATACTGAAACCGATATTCTCAAACACATCGACGTGCTCGACCCGAGTGCTTTGTGTGATGAAGATGGATGGCTGTTGAAAAATCCAATGGGAATAAGGACTGAAAGAGAAGTTCACGCGGAATCAGAGGGTGCGAAAATTTATAGAAGAATGTATCAAAAGTTTGCATAA

Coding sequence (CDS)

ATGGCTTCGTCACTTTGCGTCCAATTACAAACTTGCTCGCAGCTCCGTTGCATTGCTGCTGCAACGGCGGCGGCGGCGGTGGCCCCGTCGTCGAGGGTCGCCACTGCAAGCCGAGCCTTATCCCCGCTCCTTAAACGACCATTTCTTCGAAACTTCTGTTCACCATCCGCCTCTTCCGCCGACCTTTGCGATCGACAACAACTCCGGAGTCCGGAGCTGGTGGCGTTGGAATATGCAGACCTTAATTTCTCTCCCAAGATCTTTCAGGATTCGGGTCACGTAAGAATCAGGCAGCATGTTAATCCTCTTAGTTCTTCTTTCTCTGTCCCTACACCAGCACCCAATTGGGATGAAGTTTTCAAGGATCCCACATTGCCTCTTATGGTTGATATAGGAAGTGGTAGTGGCAGATTTCTCTTGTGGCTTGCAAAAAAACATCTTGATTCAAGAAATTTTTTGGGTCTCGAAATACGACAGAAACTGGTCAAGCGTGCTGATGTCTGGGTCAAAGAGTTGGGATTTAGCAACGTACACTTCATGTTTGCAAATGCTACAATTTCCTTTGAACAGATTATATCTTCATATCCTGGACCCCTGATTCTGGTTTCCATACTGTGCCCAGATCCTCATTTCAAGAAACGGCATCATAAGCGAAGGGTTGTACAGAAGCCTTTAATAGAATCTATTTTAGATAGTTTAATGCCTGGAGGGCAGATTTTCATACAATCTGATGTGCTTGAAGTGGCAGTTGACATGAGACTTTGGTTCGATACTGAAACCGATATTCTCAAACACATCGACGTGCTCGACCCGAGTGCTTTGTGTGATGAAGATGGATGGCTGTTGAAAAATCCAATGGGAATAAGGACTGAAAGAGAAGTTCACGCGGAATCAGAGGGTGCGAAAATTTATAGAAGAATGTATCAAAAGTTTGCATAA

Protein sequence

MASSLCVQLQTCSQLRCIAAATAAAAVAPSSRVATASRALSPLLKRPFLRNFCSPSASSADLCDRQQLRSPELVALEYADLNFSPKIFQDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFSNVHFMFANATISFEQIISSYPGPLILVSILCPDPHFKKRHHKRRVVQKPLIESILDSLMPGGQIFIQSDVLEVAVDMRLWFDTETDILKHIDVLDPSALCDEDGWLLKNPMGIRTEREVHAESEGAKIYRRMYQKFA
Homology
BLAST of Sed0004515 vs. NCBI nr
Match: XP_023527130.1 (uncharacterized protein LOC111790461 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 555.8 bits (1431), Expect = 2.3e-154
Identity = 274/311 (88.10%), Postives = 294/311 (94.53%), Query Frame = 0

Query: 1   MASSLCVQLQTCSQLRCIAAATAAAAVAPSSRVATASRALSPLLKRPFLRNFCSPSASSA 60
           MASSLC QL TCSQLRCIAAA AAA  APSS VATA+R LS LLKRPFL++FC PS SSA
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAVAAPSS-VATANRTLSLLLKRPFLQSFCLPSGSSA 60

Query: 61  DLCDRQQLRSPELVALEYADLNFSPKIFQDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120
           +LC+RQQLRSPELVALEYADLN S KIF+DSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF
Sbjct: 61  ELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120

Query: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFSNVHFM 180
           KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGF+NVHFM
Sbjct: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFM 180

Query: 181 FANATISFEQIISSYPGPLILVSILCPDPHFKKRHHKRRVVQKPLIESILDSLMPGGQIF 240
           FANATISF+QIISSYPGPL+LVSILCPDPHFKKRHHKRRV+QKPL++SI+DSLMPGGQ+F
Sbjct: 181 FANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVF 240

Query: 241 IQSDVLEVAVDMRLWFDTETDILKHIDVLDPSALCDEDGWLLKNPMGIRTEREVHAESEG 300
           IQSDVLEVAVDMRLWFD+  D+LKH+DVLD S LCD+DGWLLKNPMGIRTERE+HAESEG
Sbjct: 241 IQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKDGWLLKNPMGIRTEREIHAESEG 300

Query: 301 AKIYRRMYQKF 312
           A IYRRMYQK+
Sbjct: 301 ANIYRRMYQKY 310

BLAST of Sed0004515 vs. NCBI nr
Match: KAG7018929.1 (trmB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 555.1 bits (1429), Expect = 3.9e-154
Identity = 274/311 (88.10%), Postives = 295/311 (94.86%), Query Frame = 0

Query: 1   MASSLCVQLQTCSQLRCIAAATAAAAVAPSSRVATASRALSPLLKRPFLRNFCSPSASSA 60
           MASSLC QL TCSQLRCIAAA AAAA APSS VATA+R LS L+KRPFL++FC PS SSA
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSS-VATANRTLSLLVKRPFLQSFCLPSGSSA 60

Query: 61  DLCDRQQLRSPELVALEYADLNFSPKIFQDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120
           +LC+RQQLRSPELVALEYADLN S KIF+DSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF
Sbjct: 61  ELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120

Query: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFSNVHFM 180
           KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGF+NVHFM
Sbjct: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFM 180

Query: 181 FANATISFEQIISSYPGPLILVSILCPDPHFKKRHHKRRVVQKPLIESILDSLMPGGQIF 240
           FANATISF+QIISSYPGPL+LVSILCPDPHFKKRHHKRRV+QKPL++SI+DSLMPGGQ+F
Sbjct: 181 FANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVF 240

Query: 241 IQSDVLEVAVDMRLWFDTETDILKHIDVLDPSALCDEDGWLLKNPMGIRTEREVHAESEG 300
           IQSDVLEVAVDMRLWFD+  D+LKH+DVLD S LCD++GWLLKNPMGIRTEREVHAESEG
Sbjct: 241 IQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEG 300

Query: 301 AKIYRRMYQKF 312
           A IYRRMYQK+
Sbjct: 301 ANIYRRMYQKY 310

BLAST of Sed0004515 vs. NCBI nr
Match: XP_022924717.1 (uncharacterized protein LOC111432133 [Cucurbita moschata])

HSP 1 Score: 551.6 bits (1420), Expect = 4.4e-153
Identity = 272/311 (87.46%), Postives = 294/311 (94.53%), Query Frame = 0

Query: 1   MASSLCVQLQTCSQLRCIAAATAAAAVAPSSRVATASRALSPLLKRPFLRNFCSPSASSA 60
           MASSLC QL TCSQLRCIAAA AAAA APSS VATA+R LS L+KRPFL++FC PS SSA
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSS-VATANRTLSLLVKRPFLQSFCLPSGSSA 60

Query: 61  DLCDRQQLRSPELVALEYADLNFSPKIFQDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120
           +LC+RQQLRSPELVALEYADLN S KIF+DSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF
Sbjct: 61  ELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120

Query: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFSNVHFM 180
           KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGF+NVHFM
Sbjct: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFM 180

Query: 181 FANATISFEQIISSYPGPLILVSILCPDPHFKKRHHKRRVVQKPLIESILDSLMPGGQIF 240
           FANATISF+QIISSYPGPL+LVSILCPDPHFKKRHHKRRV+QKPL++SI+DSLMPGGQ+F
Sbjct: 181 FANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVF 240

Query: 241 IQSDVLEVAVDMRLWFDTETDILKHIDVLDPSALCDEDGWLLKNPMGIRTEREVHAESEG 300
           IQSDVLEVAVDMRLWFD+  ++LKH+DV D S LCD+DGWLLKNPMGIRTERE+HAESEG
Sbjct: 241 IQSDVLEVAVDMRLWFDSVPNVLKHVDVHDQSLLCDKDGWLLKNPMGIRTEREIHAESEG 300

Query: 301 AKIYRRMYQKF 312
           A IYRRMYQK+
Sbjct: 301 ANIYRRMYQKY 310

BLAST of Sed0004515 vs. NCBI nr
Match: KAG6582546.1 (hypothetical protein SDJN03_22548, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 549.7 bits (1415), Expect = 1.7e-152
Identity = 272/311 (87.46%), Postives = 293/311 (94.21%), Query Frame = 0

Query: 1   MASSLCVQLQTCSQLRCIAAATAAAAVAPSSRVATASRALSPLLKRPFLRNFCSPSASSA 60
           MASSLC QL TCSQLRCIAAA AAAA APSS VATA+R LS L+KRPFL++FC PS SSA
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSS-VATANRTLSLLVKRPFLQSFCLPSDSSA 60

Query: 61  DLCDRQQLRSPELVALEYADLNFSPKIFQDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120
           +LC+RQQLRSPELVALEYADLN S KIF+DSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF
Sbjct: 61  ELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120

Query: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFSNVHFM 180
           KDP LPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGF+NVHFM
Sbjct: 121 KDPKLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFM 180

Query: 181 FANATISFEQIISSYPGPLILVSILCPDPHFKKRHHKRRVVQKPLIESILDSLMPGGQIF 240
           FANATISF+QIISSYPGPL+LVSILCPDPHFKKRHHKRRV+QKPL++SI+DSLMPGGQ+F
Sbjct: 181 FANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVF 240

Query: 241 IQSDVLEVAVDMRLWFDTETDILKHIDVLDPSALCDEDGWLLKNPMGIRTEREVHAESEG 300
           IQSDVLEVAVDMRLWFD+  ++LKH DVLD S LCD+DGWLLKNPMGIRTERE+HAESEG
Sbjct: 241 IQSDVLEVAVDMRLWFDSVPNVLKHADVLDQSLLCDKDGWLLKNPMGIRTEREIHAESEG 300

Query: 301 AKIYRRMYQKF 312
           A IYRRMYQK+
Sbjct: 301 ANIYRRMYQKY 310

BLAST of Sed0004515 vs. NCBI nr
Match: XP_022980361.1 (uncharacterized protein LOC111479752 [Cucurbita maxima])

HSP 1 Score: 549.7 bits (1415), Expect = 1.7e-152
Identity = 271/311 (87.14%), Postives = 292/311 (93.89%), Query Frame = 0

Query: 1   MASSLCVQLQTCSQLRCIAAATAAAAVAPSSRVATASRALSPLLKRPFLRNFCSPSASSA 60
           MASSLC QL TCSQLRCIAAA AAAA APSS VATA+R LS L+KRPFL +FC PS SSA
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSS-VATANRTLSLLVKRPFLHSFCLPSGSSA 60

Query: 61  DLCDRQQLRSPELVALEYADLNFSPKIFQDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120
           +LC+RQQLRSPELVALEYADLN S KIF+DSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF
Sbjct: 61  ELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120

Query: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFSNVHFM 180
           KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGF+NVHFM
Sbjct: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFM 180

Query: 181 FANATISFEQIISSYPGPLILVSILCPDPHFKKRHHKRRVVQKPLIESILDSLMPGGQIF 240
           FANATISF+QIISSYPGPL+LVSILCPDPHFKKRHHKRRV+QKPL++SI+DSL PGGQ+F
Sbjct: 181 FANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLKPGGQVF 240

Query: 241 IQSDVLEVAVDMRLWFDTETDILKHIDVLDPSALCDEDGWLLKNPMGIRTEREVHAESEG 300
           IQSDVLEVAVDMRLWFD+  ++LKH+DVLD S LCD+DGWLL NPMGIRTERE+HAESEG
Sbjct: 241 IQSDVLEVAVDMRLWFDSVPNVLKHVDVLDQSLLCDKDGWLLNNPMGIRTEREIHAESEG 300

Query: 301 AKIYRRMYQKF 312
           A IYRRMYQK+
Sbjct: 301 ANIYRRMYQKY 310

BLAST of Sed0004515 vs. ExPASy Swiss-Prot
Match: Q2JJQ0 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=trmB PE=3 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.4e-45
Identity = 102/214 (47.66%), Postives = 131/214 (61.21%), Query Frame = 0

Query: 92  GHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRN 151
           GH R+RQHVNPL + +  P P P+W+ V++    P  +DIG+GSGRFLL +A++  D  N
Sbjct: 35  GH-RVRQHVNPLQAQYQQPAPPPHWERVYRRLGQPFHLDIGTGSGRFLLRIAQEQPD-WN 94

Query: 152 FLGLEIRQKLVKRADVWVKELGFSNVHFMFANATISFEQIISSYPGPLILVSILCPDPHF 211
           FLG+EIRQ LV+RA+ W  ELG  NVHF+FAN  +S   + +  PG L  V+IL PDP F
Sbjct: 95  FLGVEIRQALVERANAWRDELGLDNVHFLFANINVSLRHLFA--PGDLSRVTILFPDPWF 154

Query: 212 KKRHHKRRVVQKPLIESILDSLMPGGQIFIQSDVLEVAVDMRLWFDTETDILKHIDVLDP 271
           KKRHHKRR+VQ  L+  +   L PG  +F+QSD+ EVA +M   F      L+H    DP
Sbjct: 155 KKRHHKRRIVQPQLVADLALLLRPGSPVFLQSDIQEVAEEMAARF------LEHPQFWDP 214

Query: 272 SALCDEDGWLLKNPMGIRTEREVHAESEGAKIYR 306
                  G L  NP+GI TERE         IYR
Sbjct: 215 -----HQGPLDSNPLGIPTEREWQCLQLDLPIYR 233

BLAST of Sed0004515 vs. ExPASy Swiss-Prot
Match: Q2JRH1 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=trmB PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 9.1e-45
Identity = 102/211 (48.34%), Postives = 130/211 (61.61%), Query Frame = 0

Query: 95  RIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLG 154
           R+RQHVNPL + +  PTP P+W+ V++    PL +DIGSGSGRFLL +A++  D  NFLG
Sbjct: 34  RLRQHVNPLQAQYQQPTPPPHWERVYRRLGQPLHLDIGSGSGRFLLRMAQEQPD-WNFLG 93

Query: 155 LEIRQKLVKRADVWVKELGFSNVHFMFANATISFEQIISSYPGPLILVSILCPDPHFKKR 214
           +EIRQ LV+RA+ W  ELG  NVHF+FAN  +S   + +  PG L  V+I  PDP FKKR
Sbjct: 94  VEIRQPLVERANAWRDELGLDNVHFLFANINVSLRHLFA--PGDLSRVTIQFPDPWFKKR 153

Query: 215 HHKRRVVQKPLIESILDSLMPGGQIFIQSDVLEVAVDMRLWFDTETDILKHIDVLDPSAL 274
           HHKRR+VQ  L+  +   L PG  +F+QSDV EVA +M   F      L+H    +P   
Sbjct: 154 HHKRRIVQPRLVADLALLLQPGSPVFLQSDVREVAEEMVDRF------LEHPQFWNP--- 213

Query: 275 CDEDGWLLKNPMGIRTEREVHAESEGAKIYR 306
               G +  NP GI TERE      G  IYR
Sbjct: 214 --YQGPIDDNPFGIPTEREWQCLQLGLPIYR 230

BLAST of Sed0004515 vs. ExPASy Swiss-Prot
Match: P73161 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=trmB PE=3 SV=2)

HSP 1 Score: 167.5 bits (423), Expect = 2.3e-40
Identity = 92/218 (42.20%), Postives = 129/218 (59.17%), Query Frame = 0

Query: 94  VRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFL 153
           VRIRQHVNPLS  +      P+W  V+ D   PL +DIG   GRF L +A++H D  NFL
Sbjct: 4   VRIRQHVNPLSEKYRQVLACPDWATVYDDVQRPLHLDIGCARGRFPLKMAQQHPD-WNFL 63

Query: 154 GLEIRQKLVKRADVWVKELGFSNVHFMFANATISFEQIISSYPGPLILVSILCPDPHFKK 213
           G+EIRQ LV  A+     LG  N+H++F N  +  E+  S++P  L  VSI  PDP FK+
Sbjct: 64  GVEIRQPLVLEANETGDRLGLKNLHYLFGNINVEPEKFFSAFPPTLQRVSIQFPDPWFKQ 123

Query: 214 RHHKRRVVQKPLIESILDSLMPGGQIFIQSDVLEVAVDMRLWF-DTETDILKHIDVLDPS 273
           RH+KRRV Q  L+ +I ++L PGG++ +QSDV  VA DMR  F + +  +  H       
Sbjct: 124 RHNKRRVAQPELVTAIANALPPGGEVLLQSDVEPVAQDMRDRFAENDNFVFTH------- 183

Query: 274 ALCDEDGWLLKNPMGIRTEREVHAESEGAKIYRRMYQK 311
               +  WL +NP+G+ TERE+   +    +YR + Q+
Sbjct: 184 ----DTPWLAENPLGVPTEREIACFNLQRPVYRCLLQR 209

BLAST of Sed0004515 vs. ExPASy Swiss-Prot
Match: P72546 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=trmB PE=3 SV=2)

HSP 1 Score: 163.3 bits (412), Expect = 4.4e-39
Identity = 91/216 (42.13%), Postives = 129/216 (59.72%), Query Frame = 0

Query: 94  VRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFL 153
           VR+RQHVNPLS  F V T  P+W +V+ D   PL +DIG   GRFLL +A       N+L
Sbjct: 4   VRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQ-PEWNYL 63

Query: 154 GLEIRQKLVKRADVWVKELGFSNVHFMFANATISFEQIISSYP-GPLILVSILCPDPHFK 213
           GLEIR+ LV  A+   +E   +N+++ F+NA +  E ++ S P G L  VSI  PDP FK
Sbjct: 64  GLEIREPLVDEANAIARERELTNLYYHFSNANLDLEPLLRSLPTGILQRVSIQFPDPWFK 123

Query: 214 KRHHKRRVVQKPLIESILDSLMPGGQIFIQSDVLEVAVDMRLWFDTETDILKHIDVLDPS 273
           KRH KRRVVQ  L++++  +L  G ++F+QSDVLEV  +M   F  E    +    LD  
Sbjct: 124 KRHQKRRVVQPELVQALATALPAGAEVFLQSDVLEVQAEMCEHFAAEPRFQR--TCLD-- 183

Query: 274 ALCDEDGWLLKNPMGIRTEREVHAESEGAKIYRRMY 309
                  WL +NP+ + TERE+  +++   +YR ++
Sbjct: 184 -------WLPENPLPVPTEREIAVQNKQLPVYRALF 207

BLAST of Sed0004515 vs. ExPASy Swiss-Prot
Match: Q5N2X5 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=trmB PE=3 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 4.4e-39
Identity = 91/216 (42.13%), Postives = 129/216 (59.72%), Query Frame = 0

Query: 94  VRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFL 153
           VR+RQHVNPLS  F V T  P+W +V+ D   PL +DIG   GRFLL +A       N+L
Sbjct: 4   VRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQ-PEWNYL 63

Query: 154 GLEIRQKLVKRADVWVKELGFSNVHFMFANATISFEQIISSYP-GPLILVSILCPDPHFK 213
           GLEIR+ LV  A+   +E   +N+++ F+NA +  E ++ S P G L  VSI  PDP FK
Sbjct: 64  GLEIREPLVDEANAIARERELTNLYYHFSNANLDLEPLLRSLPTGILQRVSIQFPDPWFK 123

Query: 214 KRHHKRRVVQKPLIESILDSLMPGGQIFIQSDVLEVAVDMRLWFDTETDILKHIDVLDPS 273
           KRH KRRVVQ  L++++  +L  G ++F+QSDVLEV  +M   F  E    +    LD  
Sbjct: 124 KRHQKRRVVQPELVQALATALPAGAEVFLQSDVLEVQAEMCEHFAAEPRFQR--TCLD-- 183

Query: 274 ALCDEDGWLLKNPMGIRTEREVHAESEGAKIYRRMY 309
                  WL +NP+ + TERE+  +++   +YR ++
Sbjct: 184 -------WLPENPLPVPTEREIAVQNKQLPVYRALF 207

BLAST of Sed0004515 vs. ExPASy TrEMBL
Match: A0A6J1E9S1 (uncharacterized protein LOC111432133 OS=Cucurbita moschata OX=3662 GN=LOC111432133 PE=4 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.1e-153
Identity = 272/311 (87.46%), Postives = 294/311 (94.53%), Query Frame = 0

Query: 1   MASSLCVQLQTCSQLRCIAAATAAAAVAPSSRVATASRALSPLLKRPFLRNFCSPSASSA 60
           MASSLC QL TCSQLRCIAAA AAAA APSS VATA+R LS L+KRPFL++FC PS SSA
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSS-VATANRTLSLLVKRPFLQSFCLPSGSSA 60

Query: 61  DLCDRQQLRSPELVALEYADLNFSPKIFQDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120
           +LC+RQQLRSPELVALEYADLN S KIF+DSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF
Sbjct: 61  ELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120

Query: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFSNVHFM 180
           KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGF+NVHFM
Sbjct: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFM 180

Query: 181 FANATISFEQIISSYPGPLILVSILCPDPHFKKRHHKRRVVQKPLIESILDSLMPGGQIF 240
           FANATISF+QIISSYPGPL+LVSILCPDPHFKKRHHKRRV+QKPL++SI+DSLMPGGQ+F
Sbjct: 181 FANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVF 240

Query: 241 IQSDVLEVAVDMRLWFDTETDILKHIDVLDPSALCDEDGWLLKNPMGIRTEREVHAESEG 300
           IQSDVLEVAVDMRLWFD+  ++LKH+DV D S LCD+DGWLLKNPMGIRTERE+HAESEG
Sbjct: 241 IQSDVLEVAVDMRLWFDSVPNVLKHVDVHDQSLLCDKDGWLLKNPMGIRTEREIHAESEG 300

Query: 301 AKIYRRMYQKF 312
           A IYRRMYQK+
Sbjct: 301 ANIYRRMYQKY 310

BLAST of Sed0004515 vs. ExPASy TrEMBL
Match: A0A6J1ITE1 (uncharacterized protein LOC111479752 OS=Cucurbita maxima OX=3661 GN=LOC111479752 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.0e-153
Identity = 271/311 (87.14%), Postives = 292/311 (93.89%), Query Frame = 0

Query: 1   MASSLCVQLQTCSQLRCIAAATAAAAVAPSSRVATASRALSPLLKRPFLRNFCSPSASSA 60
           MASSLC QL TCSQLRCIAAA AAAA APSS VATA+R LS L+KRPFL +FC PS SSA
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSS-VATANRTLSLLVKRPFLHSFCLPSGSSA 60

Query: 61  DLCDRQQLRSPELVALEYADLNFSPKIFQDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120
           +LC+RQQLRSPELVALEYADLN S KIF+DSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF
Sbjct: 61  ELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120

Query: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFSNVHFM 180
           KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGF+NVHFM
Sbjct: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFM 180

Query: 181 FANATISFEQIISSYPGPLILVSILCPDPHFKKRHHKRRVVQKPLIESILDSLMPGGQIF 240
           FANATISF+QIISSYPGPL+LVSILCPDPHFKKRHHKRRV+QKPL++SI+DSL PGGQ+F
Sbjct: 181 FANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLKPGGQVF 240

Query: 241 IQSDVLEVAVDMRLWFDTETDILKHIDVLDPSALCDEDGWLLKNPMGIRTEREVHAESEG 300
           IQSDVLEVAVDMRLWFD+  ++LKH+DVLD S LCD+DGWLL NPMGIRTERE+HAESEG
Sbjct: 241 IQSDVLEVAVDMRLWFDSVPNVLKHVDVLDQSLLCDKDGWLLNNPMGIRTEREIHAESEG 300

Query: 301 AKIYRRMYQKF 312
           A IYRRMYQK+
Sbjct: 301 ANIYRRMYQKY 310

BLAST of Sed0004515 vs. ExPASy TrEMBL
Match: A0A6J1CZT2 (uncharacterized protein LOC111015991 OS=Momordica charantia OX=3673 GN=LOC111015991 PE=4 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.3e-142
Identity = 259/313 (82.75%), Postives = 279/313 (89.14%), Query Frame = 0

Query: 1   MASSLCVQLQTCSQLRCIAAATAAAAVAPSSRVATASRALSPL--LKRPFLRNFCSPSAS 60
           MA SLC QL TCSQL CIAAA AA   APSS VATA+R LSP   L+RPFL +FC     
Sbjct: 1   MAPSLCFQLHTCSQLGCIAAAKAA---APSS-VATANRTLSPFLRLRRPFLHSFCVRLGP 60

Query: 61  SADLCDRQQLRSPELVALEYADLNFSPKIFQDSGHVRIRQHVNPLSSSFSVPTPAPNWDE 120
           SA++ + Q LRSPELVALEYADLN   +IF+DSGHVRIRQHVNPLSSSF+VP PAPNW+E
Sbjct: 61  SAEVFEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNE 120

Query: 121 VFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFSNVH 180
           VFKDPTLPLMVDIGSGSGRFLLWLAK+HLD RNFLGLEIR KLVKRAD+WVKELG SNVH
Sbjct: 121 VFKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVH 180

Query: 181 FMFANATISFEQIISSYPGPLILVSILCPDPHFKKRHHKRRVVQKPLIESILDSLMPGGQ 240
           FMFANATISF+QIISSYPGPL+LVSILCPDPHFKKRHHKRRVVQKPL+ESI+DSL PGGQ
Sbjct: 181 FMFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQ 240

Query: 241 IFIQSDVLEVAVDMRLWFDTETDILKHIDVLDPSALCDEDGWLLKNPMGIRTEREVHAES 300
           + IQSDVLEVAVDMRLWFD+E D+LKHIDVLDPS LCDEDGWLL NPMGIRTERE+HAES
Sbjct: 241 VLIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAES 300

Query: 301 EGAKIYRRMYQKF 312
           EGAKIYRRMYQKF
Sbjct: 301 EGAKIYRRMYQKF 309

BLAST of Sed0004515 vs. ExPASy TrEMBL
Match: A0A1S3AVT2 (tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 PE=4 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 2.4e-141
Identity = 256/310 (82.58%), Postives = 276/310 (89.03%), Query Frame = 0

Query: 1   MASSLCVQLQTCSQLRCIAAATAAAAVAPSSRVATASRALSPLLKRPFLRNFCSPSASSA 60
           MASSLC+ LQTCS LRC+AAA  AA     S VATA++ LS   KRPFLRNF  PS SSA
Sbjct: 1   MASSLCLHLQTCSHLRCLAAAKPAA----PSVVATANKILS---KRPFLRNFSLPSGSSA 60

Query: 61  DLCDRQQLRSPELVALEYADLNFSPKIFQDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120
           DLC+RQ LRS ELVALEYADLN S K+ +D GHVRIRQHVNPLSSSFSVPTPAPNWDEVF
Sbjct: 61  DLCERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120

Query: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFSNVHFM 180
           +DPTLPLMVDIGSGSGRFLLWLAK HL+SRNFLGLEIRQKLVKRA  WVKELGFSNVHFM
Sbjct: 121 RDPTLPLMVDIGSGSGRFLLWLAKSHLESRNFLGLEIRQKLVKRAGFWVKELGFSNVHFM 180

Query: 181 FANATISFEQIISSYPGPLILVSILCPDPHFKKRHHKRRVVQKPLIESILDSLMPGGQIF 240
           FANATISF+QIISSYPGPL+LVSILCPDPHFKKRHHKRRV+QKPL+ESI+DSLMPGGQ+F
Sbjct: 181 FANATISFKQIISSYPGPLLLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVF 240

Query: 241 IQSDVLEVAVDMRLWFDTETDILKHIDVLDPSALCDEDGWLLKNPMGIRTEREVHAESEG 300
           IQSDVLEVA DMRLWFD+E DIL+HID +D S LCD+DGWLL NPMGIRTERE+HAESEG
Sbjct: 241 IQSDVLEVAADMRLWFDSEVDILEHIDKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEG 300

Query: 301 AKIYRRMYQK 311
           A IYRRMY K
Sbjct: 301 ANIYRRMYSK 303

BLAST of Sed0004515 vs. ExPASy TrEMBL
Match: A0A5A7U1F8 (tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003710 PE=4 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 4.1e-141
Identity = 255/310 (82.26%), Postives = 276/310 (89.03%), Query Frame = 0

Query: 1   MASSLCVQLQTCSQLRCIAAATAAAAVAPSSRVATASRALSPLLKRPFLRNFCSPSASSA 60
           MASSLC+ LQTCS LRC+ AA  AA     S VATA++ LS   KRPFLRNF  PS SSA
Sbjct: 1   MASSLCLHLQTCSHLRCLGAAKPAA----PSVVATANKILS---KRPFLRNFSLPSGSSA 60

Query: 61  DLCDRQQLRSPELVALEYADLNFSPKIFQDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120
           DLC+RQ LRS ELVALEYADLN S K+ +D GHVRIRQHVNPLSSSFSVPTPAPNWDEVF
Sbjct: 61  DLCERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVF 120

Query: 121 KDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFSNVHFM 180
           +DPTLPLMVDIGSGSGRFLLWLAK+HL+SRNFLGLEIRQKLVKRA  WVKELGFSNVHFM
Sbjct: 121 RDPTLPLMVDIGSGSGRFLLWLAKRHLESRNFLGLEIRQKLVKRAGFWVKELGFSNVHFM 180

Query: 181 FANATISFEQIISSYPGPLILVSILCPDPHFKKRHHKRRVVQKPLIESILDSLMPGGQIF 240
           FANATISF+QIISSYPGPL+LVSILCPDPHFKKRHHKRRV+QKPL+ESI+DSLMPGGQ+F
Sbjct: 181 FANATISFKQIISSYPGPLLLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVF 240

Query: 241 IQSDVLEVAVDMRLWFDTETDILKHIDVLDPSALCDEDGWLLKNPMGIRTEREVHAESEG 300
           IQSDVLEVA DMRLWFD+E DIL+HID +D S LCD+DGWLL NPMGIRTERE+HAESEG
Sbjct: 241 IQSDVLEVAADMRLWFDSEVDILEHIDKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEG 300

Query: 301 AKIYRRMYQK 311
           A IYRRMY K
Sbjct: 301 ANIYRRMYSK 303

BLAST of Sed0004515 vs. TAIR 10
Match: AT5G17660.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 375.9 bits (964), Expect = 3.1e-104
Identity = 184/265 (69.43%), Postives = 217/265 (81.89%), Query Frame = 0

Query: 46  RPFLRNFCSPSASSADLCDRQQLRSPELVALEYADLNFSPKIFQDSGHVRIRQHVNPLSS 105
           R F     S SAS++   D + LRS ELV LEYA+LN + KI ++ GHVRIRQHVNPLSS
Sbjct: 46  RAFNAVSASSSASTSVCVDFKDLRSNELVELEYAELNLNHKISEEVGHVRIRQHVNPLSS 105

Query: 106 SFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRA 165
           SFS P P P WDEV+KDP+LPLMVDIGSGSGRFLLW A K+++SRN+LGLEIRQKLVKRA
Sbjct: 106 SFSKPAPVPVWDEVYKDPSLPLMVDIGSGSGRFLLWQANKNVESRNYLGLEIRQKLVKRA 165

Query: 166 DVWVKELGFSNVHFMFANATISFEQIISSYPGPLILVSILCPDPHFKKRHHKRRVVQKPL 225
           + WV ELG SNVHF+FANA +SFE +ISSYPGPL +VSILCPDPHFKKRH KRRVVQKPL
Sbjct: 166 NFWVNELGLSNVHFIFANAMVSFEHLISSYPGPLEIVSILCPDPHFKKRHQKRRVVQKPL 225

Query: 226 IESILDSLMPGGQIFIQSDVLEVAVDMRLWFDTETDILKHIDVLDPSALCDEDGWLLKNP 285
           + SIL +L PGG+IF+QSDVL+VA DMR   D E+++L+H+D +D      EDGWL +NP
Sbjct: 226 VNSILQNLKPGGKIFVQSDVLDVAQDMRDQLDEESNVLQHMDTVD-----TEDGWLTENP 285

Query: 286 MGIRTEREVHAESEGAKIYRRMYQK 311
           MGIRTERE+HAE EGA+IYRR+YQK
Sbjct: 286 MGIRTEREIHAEFEGARIYRRLYQK 305

BLAST of Sed0004515 vs. TAIR 10
Match: AT5G24840.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 63.9 bits (154), Expect = 2.6e-10
Identity = 50/175 (28.57%), Postives = 78/175 (44.57%), Query Frame = 0

Query: 95  RIRQHVNPLSSS-FSVPTPAPNWDEVFKDPTL----------PLMVDIGSGSGRFLLWLA 154
           R R H NPLS S F +P    + D     P                DIG G G  L+ LA
Sbjct: 23  RARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNKFIKKVEFADIGCGFGGLLISLA 82

Query: 155 KKHLDSRNFLGLEIRQKLVKRADVWVKELGFSNVHFMFANATISFEQIISSYP-----GP 214
               D+   +G+E+R K+ +     +  L  ++    + N ++     +   P     G 
Sbjct: 83  TLFPDTL-MIGMELRDKVTEYVKERILALRRTSSEGQYENISVVRTNSMKYIPNYFEKGQ 142

Query: 215 LILVSILCPDPHFKKRHHKRRVVQKPLIESILDSLMPGGQIFIQSDVLEVAVDMR 254
           L  +  L PDPHFK+++H+RRV+   L++     L  GG I+  +DV E+   M+
Sbjct: 143 LSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRAGGIIYTITDVEELGEWMK 196

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023527130.12.3e-15488.10uncharacterized protein LOC111790461 [Cucurbita pepo subsp. pepo][more]
KAG7018929.13.9e-15488.10trmB [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022924717.14.4e-15387.46uncharacterized protein LOC111432133 [Cucurbita moschata][more]
KAG6582546.11.7e-15287.46hypothetical protein SDJN03_22548, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022980361.11.7e-15287.14uncharacterized protein LOC111479752 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q2JJQ01.4e-4547.66tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-... [more]
Q2JRH19.1e-4548.34tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX... [more]
P731612.3e-4042.20tRNA (guanine-N(7)-)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / K... [more]
P725464.4e-3942.13tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 79... [more]
Q5N2X54.4e-3942.13tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 /... [more]
Match NameE-valueIdentityDescription
A0A6J1E9S12.1e-15387.46uncharacterized protein LOC111432133 OS=Cucurbita moschata OX=3662 GN=LOC1114321... [more]
A0A6J1ITE18.0e-15387.14uncharacterized protein LOC111479752 OS=Cucurbita maxima OX=3661 GN=LOC111479752... [more]
A0A6J1CZT21.3e-14282.75uncharacterized protein LOC111015991 OS=Momordica charantia OX=3673 GN=LOC111015... [more]
A0A1S3AVT22.4e-14182.58tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 P... [more]
A0A5A7U1F84.1e-14182.26tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT5G17660.13.1e-10469.43tRNA (guanine-N-7) methyltransferase [more]
AT5G24840.12.6e-1028.57tRNA (guanine-N-7) methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePFAMPF02390Methyltransf_4coord: 126..305
e-value: 4.7E-32
score: 110.8
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePANTHERPTHR234173-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASEcoord: 50..310
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePROSITEPS51625SAM_MT_TRMBcoord: 84..310
score: 53.22908
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 95..312
e-value: 4.5E-53
score: 181.5
NoneNo IPR availablePANTHERPTHR23417:SF21TRNA (GUANINE-N(7)-)-METHYLTRANSFERASEcoord: 50..310
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 129..242
e-value: 1.45424E-4
score: 38.5651
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 126..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0004515.1Sed0004515.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0106004 tRNA (guanine-N7)-methylation
biological_process GO:0006400 tRNA modification
cellular_component GO:0043527 tRNA methyltransferase complex
molecular_function GO:0008176 tRNA (guanine-N7-)-methyltransferase activity