Sed0004505 (gene) Chayote v1

Overview


Name	Sed0004505
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Cytochrome p450 76c2
Location	LG01: 65522747 .. 65525485 (-)
RNA-Seq Expression	Sed0004505
Synteny	Sed0004505

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAATTACCGCCAACTTAGACATTCTTTCATTTCCCCTTCTTCTTCTTCTTTTTCTACCTCTAATCTTCATTCTTCTCAAAATCTTCAATCCTCCATCGCCAAACAACCTTCCTCCCGGTCCACGCCCATGGCCGATCCTCGGAAACCTCCATCAACTCGGCCGCAACGCCCACATTTCACTAACCAACCTCGCCAAAATCCACGGCCCACTCGTGTCCCTCAAGCTAGGCTCCCAGCCCCTCGTGGTCGCCTCCTCCCCGGTCGCGGCCGCCGCGGTGCTCAAAACCGAAGACCGCCTCCTCTCGGCTCGATACATTCTCCAAATGACTCCAAACCTATCTCTCCACAACAACTCCTCCTTAGTATTCTCCCCACATTGCAACGACCACTGGAAAAGCTTACGATCAATCTGCAGAGCCAATTTGTTCAATGCCAAATCCATCGAATCGCAGGCCGGTTTGCGAGCGAGGAAGATCGACGAAATGGTGGAGTTCTTGGACTCGAAACAGGGGAGTGTTGTGGAGATTAAGGATTTTGTGTTCACCACTGTTTTCAACACCATTTCTAATCTGATCTTTTCGAGAGATTTGTTGGGGTTTTGTGGCGATGGGTTTCATGGGATTAAGGTTCCTTTTAAGAAGATGATGGATTTGGGGTTGACTCCGAATTTGGCGGATTTGTATCCATGGTTGAGACGGTTTGATCTTCAGGGGCTTCGAAGAAAAGCTCGTGTTTATAAAAACGAAGTTGATTCTTCGTGGGAAAGTTTGATTCAAGAACGAAGAAGAATTCATCTTGAAAATGGGTCCATTGTTGGTGATTTCTTGGATGTTATGATCCAAAGTGGATTTAGTGATGAACAAATCTCTCACTTGGCTGTGGTAAGTGTGTAAATTAACCAATCTTTGTTTTGTGGTTTACATAGGTTTGGTCAAATGGGATTTTTTTTAACCATTATGAAGTTTTTGAGTTGGTAAATCCACTCAATCCTTCCAATTTTCAAATCAGATCATTGTTGGTTGATATAGCTAAAGTGTGCTATATTGTTTTATTATAAAGATTTTTTTGAAAATCTCTACATTTTCGATGTGGGACTGGTTGATGGTGCTACATTCAGAACCATCATCTTGAATTAAATCTTAATTTATGGCTCAAAATAACCTATTGAATTTTACTCACTTAATTAAATTCGAATGTGTGGATCAAATATTTGATCATTTTTTTTTATCAGATTTCAATTTGCGAACCAAATATTAAATTGAAGCTCTTCTACCATTATGAATCATGTTAAAACACCAATCAACCTAAAAAAATTGGGTTGATTATGATTGAAGATAATTCAACTTAGTATGAACATAGGTCCTCTTGGATTTGAATTCCTCTCAAGATATTTCTTCTTCCGTAAATAATAATTTTCACCCTTCTAAAAAGAATATTAGTTTTCATAAGATATTTCTCAATATAGTATAATTCAATTGTTCGGTGATTTAACATAATTGAATTGATGGTTAAAATTTCGAAGTGTGCAGGGATTATTTTATAGATCCAAAGGTTAAGATGATTTCAAAATTGGAGGAGGTTTTCTTGGTCGGGTTAGAATATGCATCTCGTTCTTTATTTTGACATACTACATAATTATCTTCGGCTAGGATGTTTGACTGAGCATCATTTATTATCAAGACCTCTATGAATTTTTACAATTTTCTTGAGTGAGTTGGCATTGGATTGAAAGACATGTGTTCAAAGGAATTGATGGAGCCAAGATTTTCTTACAAAAGTTTTTACGAGTTTTTCTTATGGGAAAGGTTTATTAATTTTACGGGATTGATTGCTTTCCACCTACTTTCATGAGGTCATATACTTAATTACAAAATTCAATATTGAAAGTGTTGAAAATAACTTTTAGATATATTATCTTGAAGTTTTGTTACTTCTTGTTCGTTGCACGAAAGACTTTTCTCTTTGATAGAATATTCTTTCCTGACTTTTCTTTCTTCGGTCTCAATCGGGGTTGGGGTGAGTCCGATTTAATCTGTGTAGTTGTTCTCCATCATGTTTGTTTGTTTGTTTAATCTTCATTATATCAAAATTTGAACGAATGTATATATGAATTGCAGGAGTTATTATCAGCAGGAACGGACACAACGACAACAACAATCGAATGGGCACTAACAGAGCTACTCAAAAACAGAGCCGCACTAAGCAAAGCACAGCGAGAACTGAAAGCGGCAACCACAAAACAAAAACCCACCCTCGAAGAATCCCAAATCCCGAAGCTCCAATATCTCCAAAGCTGCATAAAAGAAGCACTGAGACTCCACCCACCGGCCCCGTTTCTCCTCCCACGCACAGCTCCACAGGCCTGCAATCTCATGGGCTACACGATTCCGAAAAACGCCCTGATTTTCGTAAACGTTTGGGGGATTGGAAGGGACCCTTCGGTCTGGGAGGACTGTGGGTCGTTCCATCCGGACAGATTTGCTGATTGTGATGCGGGTTTTAATAATGGGTTTGATTTTGGGTATTTGCCGTTCGGCGGTGGAAGGAGGATGTGCCCTGGGCTTGCCATGGCGGCCGTGCAGGTGCCTTTGATTTTAGGGACTTTGGTCCATTGCTTTGATTGGGTGTGCCCTAAGGGTGGGGATTTGGGGCATTTGGGTTTGGAGGGGAGGCTTGGGGTTACCTTGCAGAAGGATGTGCCTCTCCAACTTGTTCCTAGAAGGAGGATCTTGTGA

mRNA sequence

ATGGCAATTACCGCCAACTTAGACATTCTTTCATTTCCCCTTCTTCTTCTTCTTTTTCTACCTCTAATCTTCATTCTTCTCAAAATCTTCAATCCTCCATCGCCAAACAACCTTCCTCCCGGTCCACGCCCATGGCCGATCCTCGGAAACCTCCATCAACTCGGCCGCAACGCCCACATTTCACTAACCAACCTCGCCAAAATCCACGGCCCACTCGTGTCCCTCAAGCTAGGCTCCCAGCCCCTCGTGGTCGCCTCCTCCCCGGTCGCGGCCGCCGCGGTGCTCAAAACCGAAGACCGCCTCCTCTCGGCTCGATACATTCTCCAAATGACTCCAAACCTATCTCTCCACAACAACTCCTCCTTAGTATTCTCCCCACATTGCAACGACCACTGGAAAAGCTTACGATCAATCTGCAGAGCCAATTTGTTCAATGCCAAATCCATCGAATCGCAGGCCGGTTTGCGAGCGAGGAAGATCGACGAAATGATTAAGGATTTTGTGTTCACCACTGTTTTCAACACCATTTCTAATCTGATCTTTTCGAGAGATTTGTTGGGGTTTTGTGGCGATGGGTTTCATGGGATTAAGGTTCCTTTTAAGAAGATGATGGATTTGGGGTTGACTCCGAATTTGGCGGATTTGTATCCATGGTTGAGACGGTTTGATCTTCAGGGGCTTCGAAGAAAAGCTCGTGTTTATAAAAACGAAGTTGATTCTTCGTGGGAAAGTTTGATTCAAGAACGAAGAAGAATTCATCTTGAAAATGGGTCCATTGTTGGTGATTTCTTGGATGTTATGATCCAAAGTGGATTTAGTGATGAACAAATCTCTCACTTGGCTGTGGAGTTATTATCAGCAGGAACGGACACAACGACAACAACAATCGAATGGGCACTAACAGAGCTACTCAAAAACAGAGCCGCACTAAGCAAAGCACAGCGAGAACTGAAAGCGGCAACCACAAAACAAAAACCCACCCTCGAAGAATCCCAAATCCCGAAGCTCCAATATCTCCAAAGCTGCATAAAAGAAGCACTGAGACTCCACCCACCGGCCCCGTTTCTCCTCCCACGCACAGCTCCACAGGCCTGCAATCTCATGGGCTACACGATTCCGAAAAACGCCCTGATTTTCGTAAACGTTTGGGGGATTGGAAGGGACCCTTCGGTCTGGGAGGACTGTGGGTCGTTCCATCCGGACAGATTTGCTGATTGTGATGCGGGTTTTAATAATGGGTTTGATTTTGGGTATTTGCCGTTCGGCGGTGGAAGGAGGATGTGCCCTGGGCTTGCCATGGCGGCCGTGCAGGTGCCTTTGATTTTAGGGACTTTGGTCCATTGCTTTGATTGGGTGTGCCCTAAGGGTGGGGATTTGGGGCATTTGGGTTTGGAGGGGAGGCTTGGGGTTACCTTGCAGAAGGATGTGCCTCTCCAACTTGTTCCTAGAAGGAGGATCTTGTGA

Coding sequence (CDS)

ATGGCAATTACCGCCAACTTAGACATTCTTTCATTTCCCCTTCTTCTTCTTCTTTTTCTACCTCTAATCTTCATTCTTCTCAAAATCTTCAATCCTCCATCGCCAAACAACCTTCCTCCCGGTCCACGCCCATGGCCGATCCTCGGAAACCTCCATCAACTCGGCCGCAACGCCCACATTTCACTAACCAACCTCGCCAAAATCCACGGCCCACTCGTGTCCCTCAAGCTAGGCTCCCAGCCCCTCGTGGTCGCCTCCTCCCCGGTCGCGGCCGCCGCGGTGCTCAAAACCGAAGACCGCCTCCTCTCGGCTCGATACATTCTCCAAATGACTCCAAACCTATCTCTCCACAACAACTCCTCCTTAGTATTCTCCCCACATTGCAACGACCACTGGAAAAGCTTACGATCAATCTGCAGAGCCAATTTGTTCAATGCCAAATCCATCGAATCGCAGGCCGGTTTGCGAGCGAGGAAGATCGACGAAATGATTAAGGATTTTGTGTTCACCACTGTTTTCAACACCATTTCTAATCTGATCTTTTCGAGAGATTTGTTGGGGTTTTGTGGCGATGGGTTTCATGGGATTAAGGTTCCTTTTAAGAAGATGATGGATTTGGGGTTGACTCCGAATTTGGCGGATTTGTATCCATGGTTGAGACGGTTTGATCTTCAGGGGCTTCGAAGAAAAGCTCGTGTTTATAAAAACGAAGTTGATTCTTCGTGGGAAAGTTTGATTCAAGAACGAAGAAGAATTCATCTTGAAAATGGGTCCATTGTTGGTGATTTCTTGGATGTTATGATCCAAAGTGGATTTAGTGATGAACAAATCTCTCACTTGGCTGTGGAGTTATTATCAGCAGGAACGGACACAACGACAACAACAATCGAATGGGCACTAACAGAGCTACTCAAAAACAGAGCCGCACTAAGCAAAGCACAGCGAGAACTGAAAGCGGCAACCACAAAACAAAAACCCACCCTCGAAGAATCCCAAATCCCGAAGCTCCAATATCTCCAAAGCTGCATAAAAGAAGCACTGAGACTCCACCCACCGGCCCCGTTTCTCCTCCCACGCACAGCTCCACAGGCCTGCAATCTCATGGGCTACACGATTCCGAAAAACGCCCTGATTTTCGTAAACGTTTGGGGGATTGGAAGGGACCCTTCGGTCTGGGAGGACTGTGGGTCGTTCCATCCGGACAGATTTGCTGATTGTGATGCGGGTTTTAATAATGGGTTTGATTTTGGGTATTTGCCGTTCGGCGGTGGAAGGAGGATGTGCCCTGGGCTTGCCATGGCGGCCGTGCAGGTGCCTTTGATTTTAGGGACTTTGGTCCATTGCTTTGATTGGGTGTGCCCTAAGGGTGGGGATTTGGGGCATTTGGGTTTGGAGGGGAGGCTTGGGGTTACCTTGCAGAAGGATGTGCCTCTCCAACTTGTTCCTAGAAGGAGGATCTTGTGA

Protein sequence

MAITANLDILSFPLLLLLFLPLIFILLKIFNPPSPNNLPPGPRPWPILGNLHQLGRNAHISLTNLAKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFSPHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEMIKDFVFTTVFNTISNLIFSRDLLGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRKARVYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTDTTTTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLHPPAPFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGFNNGFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRLGVTLQKDVPLQLVPRRRIL

Homology

[Close]

BLAST of Sed0004505 vs. NCBI nr
Match: XP_031741123.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 619.4 bits (1596), Expect = 2.7e-173
Identity = 324/497 (65.19%), Postives = 384/497 (77.26%), Query Frame = 0


Query: 9   ILSFPLL---LLLFLPLIFILLKIFNPPSPNN--LPPGPRPWPILGNLHQLGRNAHISLT 68
           I SF +L   LLL +P IFI+LK    PS     LPPGP PWPILGNL Q+G+NAHIS+T
Sbjct: 3   IPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISIT 62

Query: 69  NLAKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLV 128
             A I+GPL+SLKLG+Q LVVASSP  A AVLKT DR LS RYILQMTP+ +L+N  SLV
Sbjct: 63  QFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLV 122

Query: 129 FSPHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEM-------------IKDFVFT 188
           FSP C DHWK+LRSIC+ANLF AK+IESQA LR +K+ E+             IKDFVFT
Sbjct: 123 FSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFT 182

Query: 189 TVFNTISNLIFSRDLLGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRK 248
           +VFNT+SNLIFS DLL F GDGF+GIK  FKKMMDLGLTPNLAD YP LRRFDLQGLR+K
Sbjct: 183 SVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKK 242

Query: 249 ARVYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTD 308
           A +Y NEVDS W  LI+ERR IH + GS   DFLDV+IQSGFSD+QI++L  ELLSAGTD
Sbjct: 243 AAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAGTD 302

Query: 309 TTTTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLH 368
           TTTTT+EWA++E+LK++  L+K ++E+K   TK+ P ++ES I KLQYL SC+KE LRLH
Sbjct: 303 TTTTTVEWAMSEILKDKGILNKVRQEIK-KVTKESP-IDESNISKLQYLWSCVKETLRLH 362

Query: 369 PPAPFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGF 428
           PPAPFLLPR AP  C +MGY+IPK+++IFVNVWGIGRDPS+WED  +F+P+RF D   G 
Sbjct: 363 PPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERF-DVGCGN 422

Query: 429 N---NGFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGR 485
           N    G+D+ YLPFGGGRR+CPGL MA VQVPLIL TL+H F+W  P G DL  L L GR
Sbjct: 423 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 482

[Close]

BLAST of Sed0004505 vs. NCBI nr
Match: KAE8647950.1 (hypothetical protein Csa_000545 [Cucumis sativus])

HSP 1 Score: 618.2 bits (1593), Expect = 5.9e-173
Identity = 322/500 (64.40%), Postives = 383/500 (76.60%), Query Frame = 0


Query: 9   ILSFPLL---LLLFLPLIFILLKIFNPPSPNN--LPPGPRPWPILGNLHQLGRNAHISLT 68
           I SF +L   LLL +P IFI+LK    PS     LPPGP PWPILGNL Q+G+NAHIS+T
Sbjct: 3   IPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISIT 62

Query: 69  NLAKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLV 128
             A I+GPL+SLKLG+Q LVVASSP  A AVLKT DR LS RYILQMTP+ +L+N  SLV
Sbjct: 63  QFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLV 122

Query: 129 FSPHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEM-------------IKDFVFT 188
           FSP C DHWK+LRSIC+ANLF AK+IESQA LR +K+ E+             IKDFVFT
Sbjct: 123 FSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFT 182

Query: 189 TVFNTISNLIFSRDLLGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRK 248
           +VFNT+SNLIFS DLL F GDGF+GIK  FKKMMDLGLTPNLAD YP LRRFDLQGLR+K
Sbjct: 183 SVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKK 242

Query: 249 ARVYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTD 308
           A +Y NEVDS W  LI+ERR IH + GS   DFLDV+IQSGFSD+QI++L  ELL A TD
Sbjct: 243 AAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLFAATD 302

Query: 309 TTTTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLH 368
           TTTTT+EWA++E+LK++  L+KA+ E+K    K+ P ++ES I KLQYL SC+KE LRLH
Sbjct: 303 TTTTTVEWAMSEILKDKDILNKAREEIK-KVAKESPAIDESNISKLQYLWSCVKETLRLH 362

Query: 369 PPAPFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGF 428
           PP PFLLPR AP  C +MGY+IPK+++IFVNVWGIGRDPS+WED  +F+P+RF D   G 
Sbjct: 363 PPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERF-DVGCGN 422

Query: 429 N---NGFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGR 488
           N    G+D+ YLPFGGGRR+CPGL MA VQVPLIL TL+H F+W  P G DL  L L GR
Sbjct: 423 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 482

[Close]

BLAST of Sed0004505 vs. NCBI nr
Match: XP_008467223.1 (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])

HSP 1 Score: 618.2 bits (1593), Expect = 5.9e-173
Identity = 317/499 (63.53%), Postives = 383/499 (76.75%), Query Frame = 0


Query: 9   ILSFPLL---LLLFLPLIFILLKIFNPPSPNNLPPGPRPWPILGNLHQLGRNAHISLTNL 68
           I SF +L   LLL LP+  IL  I +P S   LPPGP PWPILGNL Q+G+NAHIS+T  
Sbjct: 3   IPSFSILLPSLLLLLPIFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQF 62

Query: 69  AKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFS 128
           A I+GPL+SLKLG+Q L+VASSP AA AVLKT+DRLLSARYI QMTP+ +LH+  SLVFS
Sbjct: 63  ANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFS 122

Query: 129 PHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEM-------------IKDFVFTTV 188
           P C D WK+LRS+C+ NLF AK+IESQA LR +K+ E+             +KDFVFT+V
Sbjct: 123 PECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSV 182

Query: 189 FNTISNLIFSRDLLGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRKAR 248
           FNT+SNLIFS DLL F GDGF GIK PFKKMMDLGLTPNLAD YP LRRFDLQGL+ KA 
Sbjct: 183 FNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKAD 242

Query: 249 VYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTDTT 308
           +YKNEVDS W  LI+ERR IH + GS   DFLDV+IQSGF D+QI+ L +ELLSAGTDTT
Sbjct: 243 IYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTT 302

Query: 309 TTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLHPP 368
           TTT+EWA++E+LK++  L+K + E+K  T + +P ++ES I +LQYL  C+KE LRLHPP
Sbjct: 303 TTTVEWAMSEILKDKDILNKVREEMKKVTKENRP-IDESNISELQYLWRCVKETLRLHPP 362

Query: 369 APFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGFNN 428
           APFLLPR AP  C +MGY+IPK++++FVNVWGIGRDPSVWED  +F+P RF D   G +N
Sbjct: 363 APFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRF-DVGCGNDN 422

Query: 429 ----GFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRL 488
               G+D+ YLPFGGGRR+CPGL MA VQVPLIL TL+H F+W  P   DL  L L GRL
Sbjct: 423 VDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRL 482

[Close]

BLAST of Sed0004505 vs. NCBI nr
Match: XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 617.5 bits (1591), Expect = 1.0e-172
Identity = 316/493 (64.10%), Postives = 382/493 (77.48%), Query Frame = 0


Query: 7   LDILSFPLLLLLFLPLIFILLKIFNPPSPN-NLPPGPRPWPILGNLHQLGRNAHISLTNL 66
           ++I SF  LLLL LPLIFI+L+  +  S    LPPGP+PWPILGNL Q+G+NAHIS+T  
Sbjct: 1   MEIASFSFLLLL-LPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQF 60

Query: 67  AKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFS 126
           + I+GPL+SLKLG+Q LVVASSP AAAA+LKT+DRLLSARYI QMTP+  LH+N SL+FS
Sbjct: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFS 120

Query: 127 PHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEM-------------IKDFVFTTV 186
           P C D WK+LRSICRA LF A++IESQA LR +K++EM             IK+ VF TV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITV 180

Query: 187 FNTISNLIFSRDLLGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRKAR 246
           FNT+SN IFSRDL+GF GDGF+GIK PFK M+DLGL PNLAD YP LR FDLQGLR+KA 
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAA 240

Query: 247 VYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTDTT 306
           +YKN+VDSSWE LI+ERR IH +  S   DFLDV+IQSGF+DEQIS+L VE+LSAGTDTT
Sbjct: 241 IYKNQVDSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTT 300

Query: 307 TTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLHPP 366
           TTT+EWA+ ELLKNR  +++ + E K A       ++ES++ +L YL  C+KE LRLHPP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPP 360

Query: 367 APFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGFNN 426
           APFLLPR AP+AC LMGY++PK+ +IFVNVWGIGRDPS+WED  SF P+RF  CDA    
Sbjct: 361 APFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADL-K 420

Query: 427 GFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRLGVTL 486
           G D+ +LPFGGGRR+CPGL MAAVQVPLIL TL+H F+W  P   D   L L GRLGVTL
Sbjct: 421 GHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTL 480

[Close]

BLAST of Sed0004505 vs. NCBI nr
Match: KAA0055170.1 (putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. makuwa])

HSP 1 Score: 617.5 bits (1591), Expect = 1.0e-172
Identity = 316/499 (63.33%), Postives = 383/499 (76.75%), Query Frame = 0


Query: 9   ILSFPLL---LLLFLPLIFILLKIFNPPSPNNLPPGPRPWPILGNLHQLGRNAHISLTNL 68
           I SF +L   LLL LP+  IL  I +P S   LPPGP PWPILGNL Q+G+NAHIS+T  
Sbjct: 3   IPSFSILLPSLLLLLPIFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQF 62

Query: 69  AKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFS 128
           A I+GPL+SLKLG+Q L+VASSP AA AVLKT+DRLLSARYI QMTP+ +LH+  SLVFS
Sbjct: 63  ANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFS 122

Query: 129 PHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEM-------------IKDFVFTTV 188
           P C D WK+LRS+C+ NLF AK+IESQA LR +K+ E+             +KDFVFT+V
Sbjct: 123 PECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSV 182

Query: 189 FNTISNLIFSRDLLGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRKAR 248
           FNT+SNLIFS DL+ F GDGF GIK PFKKMMDLGLTPNLAD YP LRRFDLQGL+ KA 
Sbjct: 183 FNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKAD 242

Query: 249 VYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTDTT 308
           +YKNEVDS W  LI+ERR IH + GS   DFLDV+IQSGF D+QI+ L +ELLSAGTDTT
Sbjct: 243 IYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTT 302

Query: 309 TTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLHPP 368
           TTT+EWA++E+LK++  L+K + E+K  T + +P ++ES I +LQYL  C+KE LRLHPP
Sbjct: 303 TTTVEWAMSEILKDKDILNKVREEMKKVTKENRP-IDESNISELQYLWRCVKETLRLHPP 362

Query: 369 APFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGFNN 428
           APFLLPR AP  C +MGY+IPK++++FVNVWGIGRDPSVWED  +F+P RF D   G +N
Sbjct: 363 APFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRF-DVGCGNDN 422

Query: 429 ----GFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRL 488
               G+D+ YLPFGGGRR+CPGL MA VQVPLIL TL+H F+W  P   DL  L L GRL
Sbjct: 423 VDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRL 482

[Close]

BLAST of Sed0004505 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.3e-103
Identity = 209/484 (43.18%), Postives = 294/484 (60.74%), Query Frame = 0


Query: 17  LLFLPLIFILLKIFNPPSPNNLPPGPRPWPILGNLHQLGRNAHISLTNLAKIHGPLVSLK 76
           L+ + +   L  IF   SP  LPPGP+PWPI+GNL QLG   H     LA+ +G L SLK
Sbjct: 2   LVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLK 61

Query: 77  LGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFSPHCNDHWKSLR 136
           LGS+ +VVAS+P+AA+ +LKT DR+LS RY+ Q +  +  H  +S+V+S  CN+ WK LR
Sbjct: 62  LGSETVVVASTPLAASEILKTHDRVLSGRYVFQ-SFRVKEHVENSIVWS-ECNETWKKLR 121

Query: 137 SICRANLFNAKSIESQAGLRARKIDEMIK-------------DFVFTTVFNTISNLIFSR 196
            +CR  LF  K IESQA +R  K  EM++             + VF T+ N   NLIFS+
Sbjct: 122 KVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQ 181

Query: 197 DL--LGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRKARVYKNEVDSS 256
           ++  LG    G   +K    +M++LG + N AD +P+L +FDL G  +        + S 
Sbjct: 182 NIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSV 241

Query: 257 WESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTDTTTTTIEWALT 316
           W ++++ER+     N S   DF+++++ SG  D+QI+ L +E+  AGT+T+ +TIEWAL+
Sbjct: 242 WGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 301

Query: 317 ELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLHPPAPFLLPRTA 376
           EL KN    +  + EL +   K+   ++ES IP + YLQ+ +KE LRLHP  P LLPR A
Sbjct: 302 ELTKNPQVTANMRLELLSVVGKR--PVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRA 361

Query: 377 PQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGFNNGFDFGYLPF 436
            + C ++ YTIPK   I VN WGIGRDP  W D   F P+RF +    F  G DF  +PF
Sbjct: 362 LETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDF-KGNDFELIPF 421

Query: 437 GGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRLGVTLQKDVPLQLV 486
           G GRR+CPG+ +A   + LI+ +LV  FDW  PKG D   L +E + G+TLQK+ PL +V
Sbjct: 422 GAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIV 480

[Close]

BLAST of Sed0004505 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 2.0e-102
Identity = 202/478 (42.26%), Postives = 293/478 (61.30%), Query Frame = 0


Query: 25  ILLKIFNPPSPNNLPPGPRPWPILGNLHQLGRNAHISLTNLAKIHGPLVSLKLGSQPLVV 84
           IL  +F      NLPPGP+PWPI+GNL QLG   H     LA+ +G + +LK+G++ +VV
Sbjct: 15  ILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVV 74

Query: 85  ASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFSPHCNDHWKSLRSICRANLF 144
           AS+  AA+ +LKT DR+LSARY+ Q +  +  H  +S+V+S  C + WK+LR +CR  LF
Sbjct: 75  ASTSSAASEILKTHDRILSARYVFQ-SFRVKGHVENSIVWS-DCTETWKNLRKVCRTELF 134

Query: 145 NAKSIESQAGLRARKIDEMIK-------------DFVFTTVFNTISNLIFSRDL--LGFC 204
             K IESQA +R +K +EM++             + +F T+ N   NLIFS+++  LG  
Sbjct: 135 TQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDP 194

Query: 205 GDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRKARVYKNEVDSSWESLIQER 264
             G    K    +M++LG + N AD +P L +FDL G R++       + + W +++QER
Sbjct: 195 NSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQER 254

Query: 265 RRIHLENG-SIVGDFLDVMIQSGFSDEQISHLAVELLSAGTDTTTTTIEWALTELLKNRA 324
           +     +G     DF+DV + SG +D QI+ L +EL  AGT+T+ +TIEWA+TEL KN  
Sbjct: 255 KLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPK 314

Query: 325 ALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLHPPAPFLLPRTAPQACNLM 384
             +K + E++  T   + +++ES  P L YL++ +KE LRLHPP P LLPR A + C ++
Sbjct: 315 ITAKIRSEIQ--TVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTIL 374

Query: 385 GYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGFNNGFDFGYLPFGGGRRMC 444
            YTIPK+  I VN WGIGRDP  W D  +F P+RF +    F  G DF  +PFG GRR+C
Sbjct: 375 NYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDF-RGNDFSLIPFGAGRRIC 434

Query: 445 PGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRLGVTLQKDVPLQLVPRRRI 487
           PGL +A   + L++ T V   DW  P G  + HL +E + G+TLQK+ PL +VP+ R+
Sbjct: 435 PGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKSRV 487

[Close]

BLAST of Sed0004505 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 3.3e-102
Identity = 214/494 (43.32%), Postives = 296/494 (59.92%), Query Frame = 0


Query: 7   LDILSFPLLLLLFLPLIFILLKIFNPPSPNNLPPGPRPWPILGNLHQLGRNAHISLTNLA 66
           +++LS  ++   FL  +F +L+      P NLPPGPRP PI+GNL QLG   H     LA
Sbjct: 1   MEVLSIAIVSFSFLLFLFFILR---DSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLA 60

Query: 67  KIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFSP 126
           + +G L SLKLGSQ +VVASSP AAA +LKT D++LS RY+ Q +  +  H  +S+V+S 
Sbjct: 61  QKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQ-SFRVKEHVENSIVWS- 120

Query: 127 HCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEMIK-------------DFVFTTVF 186
            CND+WK LR +CR  LF  K IESQ+ +R  K  EM+K             + VF T+ 
Sbjct: 121 ECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLV 180

Query: 187 NTISNLIFSRDL--LGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRKA 246
           N   NLIFS+D+  L     G   +K    K++D+G + N AD +P + + DL G RR  
Sbjct: 181 NIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAV 240

Query: 247 RVYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTDT 306
                ++   W  +++ERR    +      DF+DV++ +G  D++I+ L +EL  AGT+T
Sbjct: 241 AEVLQQIYDVWGVMLKERR--GTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTET 300

Query: 307 TTTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLHP 366
           + +TIEWA+TEL K    +SK + EL         T++ES +P L YLQ+ +KE LRLHP
Sbjct: 301 SASTIEWAITELTKKPLVVSKIRLEL--VNVVGDNTVKESDLPHLPYLQAFVKETLRLHP 360

Query: 367 PAPFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGFN 426
           P P LLPR A + C +M YTIPK   I VN W IGRDP  W+D  +F P+RF   D  + 
Sbjct: 361 PTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDY- 420

Query: 427 NGFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRLGVT 486
            G DF  +PFGGGRR+CPGL +A+    LI+ TLV  F+W  P+G     L ++ + G+T
Sbjct: 421 KGNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLT 480

[Close]

BLAST of Sed0004505 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 2.2e-101
Identity = 203/496 (40.93%), Postives = 304/496 (61.29%), Query Frame = 0


Query: 7   LDILSFPLLLLLFLPLIFILLKIFNPPSPNNLPPGPRPWPILGNLHQLGRNAHISLTNLA 66
           +++++  L+ ++   ++++L   F      NLPPGP+PWPI+GNL QLG   H     LA
Sbjct: 1   MEVVTVALIAVIISSILYLL---FGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELA 60

Query: 67  KIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFSP 126
           + +G + +LK+G++ +VVAS+  AA+ +LKT DR+LSARY+ Q +  +  H  +S+V+S 
Sbjct: 61  QTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQ-SFRVKGHVENSIVWS- 120

Query: 127 HCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEMIK-------------DFVFTTVF 186
            C + WK+LR +CR  LF  K IESQA +R +K +EM++             + +F T+ 
Sbjct: 121 DCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLV 180

Query: 187 NTISNLIFSRDL--LGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRKA 246
           N   NLIFS+++  LG    G    K    +M++LG + N AD +P L +FDL G R++ 
Sbjct: 181 NIFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEV 240

Query: 247 RVYKNEVDSSWESLIQERRRIHLENG-SIVGDFLDVMIQSGFSDEQISHLAVELLSAGTD 306
                 + + W +++QER+     +G     DF+DV + SG +D QI+ L +EL  AGT+
Sbjct: 241 AECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTE 300

Query: 307 TTTTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLH 366
           T+ +TIEWA+TEL KN    +K + EL+  T   + +++ES  P L YL++ +KE LRLH
Sbjct: 301 TSASTIEWAMTELTKNPKITAKLRSELQ--TVVGERSVKESDFPNLPYLEATVKETLRLH 360

Query: 367 PPAPFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGF 426
           PP P LLPR A + C ++ YTIPK+  I VN WGIGRDP  W D  +F P+RF +    F
Sbjct: 361 PPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDF 420

Query: 427 NNGFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRLGV 486
             G DF  +PFG GRR+CPGL +A   + L++ T V   DW  P G  + HL +E + G+
Sbjct: 421 -RGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGL 480

[Close]

BLAST of Sed0004505 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.9e-97
Identity = 205/495 (41.41%), Postives = 301/495 (60.81%), Query Frame = 0


Query: 10  LSFPLLLLLFLPLIFILLKIFNPPSPNNLPPGPRPWPILGNLHQLGRNA----HISLTNL 69
           L   L  L+ + L+FILL     P   NLPPGP PWP++GNL  L  N     HI+L N+
Sbjct: 3   LQIALFSLIPVILVFILLL---KPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANM 62

Query: 70  AKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFS 129
           A+ HGP++ L LG+QP V+AS+  AA  +LKT DR+ SAR+I +M+  L  H   SLV+S
Sbjct: 63  ARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYSLVWS 122

Query: 130 PHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEM-------------IKDFVFTTV 189
             C D+WK LR I R  +F+ K +++Q+ +R +K+ E+             I  FVF T+
Sbjct: 123 -DCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTL 182

Query: 190 FNTISNLIFSRDLLGFCGDGFH--GIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRK 249
            N + N++FS+D+  + GD     GI+   ++M+ +G  PN+A+ YP L   DLQGL++K
Sbjct: 183 LNILGNVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKK 242

Query: 250 ARVYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTD 309
                  V   WE  ++ER+    E      D LDV++ + F+D QI+ L +E    G++
Sbjct: 243 CDERFIRVMKMWEGTVKERKANRNEESK---DMLDVLLANDFNDAQINALFLETFGPGSE 302

Query: 310 TTTTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLH 369
           T++ TIEW + EL+K+   ++K ++EL         T++ES +P+L YLQ+CIKEA+RLH
Sbjct: 303 TSSATIEWVIAELIKSPKEMAKVRKELNEVVGTS--TIKESDLPQLPYLQACIKEAMRLH 362

Query: 370 PPAPFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGF 429
           P APFLLPR A + C +MGYTIPKN+ + VN + IGRDP  W+D  +F P+RF + D  F
Sbjct: 363 PAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDF 422

Query: 430 NNGFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRLGV 486
            +G  + ++PFG GRR C G+ +A   +PLI+G+LVH +D+  P G     L +   L +
Sbjct: 423 -HGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSL 482

[Close]

BLAST of Sed0004505 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 2.9e-173
Identity = 317/499 (63.53%), Postives = 383/499 (76.75%), Query Frame = 0


Query: 9   ILSFPLL---LLLFLPLIFILLKIFNPPSPNNLPPGPRPWPILGNLHQLGRNAHISLTNL 68
           I SF +L   LLL LP+  IL  I +P S   LPPGP PWPILGNL Q+G+NAHIS+T  
Sbjct: 3   IPSFSILLPSLLLLLPIFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQF 62

Query: 69  AKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFS 128
           A I+GPL+SLKLG+Q L+VASSP AA AVLKT+DRLLSARYI QMTP+ +LH+  SLVFS
Sbjct: 63  ANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFS 122

Query: 129 PHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEM-------------IKDFVFTTV 188
           P C D WK+LRS+C+ NLF AK+IESQA LR +K+ E+             +KDFVFT+V
Sbjct: 123 PECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSV 182

Query: 189 FNTISNLIFSRDLLGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRKAR 248
           FNT+SNLIFS DLL F GDGF GIK PFKKMMDLGLTPNLAD YP LRRFDLQGL+ KA 
Sbjct: 183 FNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKAD 242

Query: 249 VYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTDTT 308
           +YKNEVDS W  LI+ERR IH + GS   DFLDV+IQSGF D+QI+ L +ELLSAGTDTT
Sbjct: 243 IYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTT 302

Query: 309 TTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLHPP 368
           TTT+EWA++E+LK++  L+K + E+K  T + +P ++ES I +LQYL  C+KE LRLHPP
Sbjct: 303 TTTVEWAMSEILKDKDILNKVREEMKKVTKENRP-IDESNISELQYLWRCVKETLRLHPP 362

Query: 369 APFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGFNN 428
           APFLLPR AP  C +MGY+IPK++++FVNVWGIGRDPSVWED  +F+P RF D   G +N
Sbjct: 363 APFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRF-DVGCGNDN 422

Query: 429 ----GFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRL 488
               G+D+ YLPFGGGRR+CPGL MA VQVPLIL TL+H F+W  P   DL  L L GRL
Sbjct: 423 VDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRL 482

[Close]

BLAST of Sed0004505 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 4.9e-173
Identity = 316/499 (63.33%), Postives = 383/499 (76.75%), Query Frame = 0


Query: 9   ILSFPLL---LLLFLPLIFILLKIFNPPSPNNLPPGPRPWPILGNLHQLGRNAHISLTNL 68
           I SF +L   LLL LP+  IL  I +P S   LPPGP PWPILGNL Q+G+NAHIS+T  
Sbjct: 3   IPSFSILLPSLLLLLPIFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQF 62

Query: 69  AKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFS 128
           A I+GPL+SLKLG+Q L+VASSP AA AVLKT+DRLLSARYI QMTP+ +LH+  SLVFS
Sbjct: 63  ANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFS 122

Query: 129 PHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEM-------------IKDFVFTTV 188
           P C D WK+LRS+C+ NLF AK+IESQA LR +K+ E+             +KDFVFT+V
Sbjct: 123 PECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSV 182

Query: 189 FNTISNLIFSRDLLGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRKAR 248
           FNT+SNLIFS DL+ F GDGF GIK PFKKMMDLGLTPNLAD YP LRRFDLQGL+ KA 
Sbjct: 183 FNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKAD 242

Query: 249 VYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTDTT 308
           +YKNEVDS W  LI+ERR IH + GS   DFLDV+IQSGF D+QI+ L +ELLSAGTDTT
Sbjct: 243 IYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTT 302

Query: 309 TTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLHPP 368
           TTT+EWA++E+LK++  L+K + E+K  T + +P ++ES I +LQYL  C+KE LRLHPP
Sbjct: 303 TTTVEWAMSEILKDKDILNKVREEMKKVTKENRP-IDESNISELQYLWRCVKETLRLHPP 362

Query: 369 APFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGFNN 428
           APFLLPR AP  C +MGY+IPK++++FVNVWGIGRDPSVWED  +F+P RF D   G +N
Sbjct: 363 APFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRF-DVGCGNDN 422

Query: 429 ----GFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRL 488
               G+D+ YLPFGGGRR+CPGL MA VQVPLIL TL+H F+W  P   DL  L L GRL
Sbjct: 423 VDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRL 482

[Close]

BLAST of Sed0004505 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 4.9e-173
Identity = 316/493 (64.10%), Postives = 382/493 (77.48%), Query Frame = 0


Query: 7   LDILSFPLLLLLFLPLIFILLKIFNPPSPN-NLPPGPRPWPILGNLHQLGRNAHISLTNL 66
           ++I SF  LLLL LPLIFI+L+  +  S    LPPGP+PWPILGNL Q+G+NAHIS+T  
Sbjct: 1   MEIASFSFLLLL-LPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQF 60

Query: 67  AKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFS 126
           + I+GPL+SLKLG+Q LVVASSP AAAA+LKT+DRLLSARYI QMTP+  LH+N SL+FS
Sbjct: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFS 120

Query: 127 PHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEM-------------IKDFVFTTV 186
           P C D WK+LRSICRA LF A++IESQA LR +K++EM             IK+ VF TV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITV 180

Query: 187 FNTISNLIFSRDLLGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRKAR 246
           FNT+SN IFSRDL+GF GDGF+GIK PFK M+DLGL PNLAD YP LR FDLQGLR+KA 
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAA 240

Query: 247 VYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTDTT 306
           +YKN+VDSSWE LI+ERR IH +  S   DFLDV+IQSGF+DEQIS+L VE+LSAGTDTT
Sbjct: 241 IYKNQVDSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTT 300

Query: 307 TTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLHPP 366
           TTT+EWA+ ELLKNR  +++ + E K A       ++ES++ +L YL  C+KE LRLHPP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPP 360

Query: 367 APFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGFNN 426
           APFLLPR AP+AC LMGY++PK+ +IFVNVWGIGRDPS+WED  SF P+RF  CDA    
Sbjct: 361 APFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADL-K 420

Query: 427 GFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRLGVTL 486
           G D+ +LPFGGGRR+CPGL MAAVQVPLIL TL+H F+W  P   D   L L GRLGVTL
Sbjct: 421 GHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTL 480

[Close]

BLAST of Sed0004505 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.5e-169
Identity = 310/493 (62.88%), Postives = 379/493 (76.88%), Query Frame = 0


Query: 7   LDILSFPLLLLLFLPLIFILLK-IFNPPSPNNLPPGPRPWPILGNLHQLGRNAHISLTNL 66
           ++I SF  LLLL LPLIF +L+ I +      LPPGP PWPILGNL Q+G+NAHIS+T  
Sbjct: 1   MEIASFSFLLLL-LPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQF 60

Query: 67  AKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFS 126
           +  +GPL+SLKLG+Q LVVASSP AAAA+LKT+DRLLSARYI QMTP+  LH+N SL+FS
Sbjct: 61  SHFYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFS 120

Query: 127 PHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEM-------------IKDFVFTTV 186
           P C D WK+LRSICRA LF A++IESQA LR +K+++M             IK+ VF TV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITV 180

Query: 187 FNTISNLIFSRDLLGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRKAR 246
           FNT+SN IFSRDL+GF GDGF+GIK  FK ++DLGL PNLAD YP LR FDLQGLR+KA 
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAA 240

Query: 247 VYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTDTT 306
           +YKN+VDSSWE LI+ERR IH  + S   DFLDV+I+SGF+DEQIS+L VE+LSAGTDTT
Sbjct: 241 IYKNQVDSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTT 300

Query: 307 TTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLHPP 366
           TTT+EWA+ ELLKNR  +++ + E K A       ++ES++ +L YL  C+KE LRLHPP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPP 360

Query: 367 APFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGFNN 426
           APFLLPR AP+AC LMGY++PK+ +IFVNVWGIGRDPS+WED GSF P+RF   DA    
Sbjct: 361 APFLLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADL-K 420

Query: 427 GFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRLGVTL 486
           G D+ +LPFGGGRR+CPGL MAAVQVPLIL TL+H F+W  P   D   L L GRLGVTL
Sbjct: 421 GHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTL 480

[Close]

BLAST of Sed0004505 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 2.5e-169
Identity = 308/494 (62.35%), Postives = 379/494 (76.72%), Query Frame = 0


Query: 7   LDILSFPLLLLLFLPLIFILLKIFNPPSPN-NLPPGPRPWPILGNLHQLGRNAHISLTNL 66
           ++I SF  LLL  LPLIF +LK  +  S     PPGP+PWP+LGNL Q+G+NAHIS+T  
Sbjct: 1   MEIASFSFLLL--LPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQF 60

Query: 67  AKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFS 126
           + I+GPL+SLKLG+Q LVVASSP AAAA+LKT+DRLLSARYI QM P+ +LH+N SLVFS
Sbjct: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120

Query: 127 PHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEM-------------IKDFVFTTV 186
           P C D WK+LRSICRA LF A++IESQA LR +K++EM             IK+FVF TV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180

Query: 187 FNTISNLIFSRDLLGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDLQGLRRKAR 246
           FNT+SN IFSRDL+GF GDGF+GI+  FK ++D  L PNLAD YP LR FDLQGLR+KA 
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAA 240

Query: 247 VYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFSDEQISHLAVELLSAGTDTT 306
           +YKN++DSSWE LI+ERR IH     +  DFLDV+IQSGF+DEQI++L VE+LSAGTDTT
Sbjct: 241 IYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTT 300

Query: 307 TTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRLHPP 366
           TTT+EWA+ ELLKNR  +++ + E K A       ++ES++ +L YL  C+KE LRLHPP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGD--AIDESKLSELHYLWKCVKETLRLHPP 360

Query: 367 APFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRFADCDAGFNN 426
           APFLLPR AP+AC LMGY++PK+ +I VNVWGIGRDPS+WED  SF P+RF  CDA    
Sbjct: 361 APFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADL-K 420

Query: 427 GFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGRLGVTL 486
           G D+ +LPFGGGRR+CPGL MAAVQVPLIL TL+H F+W  P   D   L L+GRLGVTL
Sbjct: 421 GHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTL 480

[Close]

BLAST of Sed0004505 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 315.5 bits (807), Expect = 7.7e-86
Identity = 184/495 (37.17%), Postives = 270/495 (54.55%), Query Frame = 0


Query: 22  LIFILLKIFNPPSPNNLPPGPRPWPILGNLHQL-GRNAHISLTNLAKIHGPLVSLKLGSQ 81
           LI++    +       LPPGP PWP++GN+ QL G   H SLT L++ HGP+++L++GS 
Sbjct: 20  LIYVTCLFYTKRCRTRLPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSM 79

Query: 82  PLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFSPHCNDHWKSLRSICR 141
             VV SS   A  + K  D  L+ R I +        ++ SL+ + +   +W+ LR +C 
Sbjct: 80  LTVVISSSEVAREIFKKHDAALAGRKIYEAMKG-GKSSDGSLITAQY-GAYWRMLRRLCT 139

Query: 142 ANLFNAKSIESQAGLRARKIDEMIK----------------DFVFTTVFNTISNLIFSRD 201
              F  + +++ + +R+R +D+M++                 + F   FN I NL+FSRD
Sbjct: 140 TQFFVTRRLDAMSDVRSRCVDQMLRFVEEGGQNGTKTIDVGRYFFLMAFNLIGNLMFSRD 199

Query: 202 LLGFCGDGFHGIKVPFK--KMMDLGLTPNLADLYPWLRRFDLQGLRRKARVYKNEVDSSW 261
           LL    D   G +  +   K+M+    PN+AD +P LR  D QG+RRK + +  +     
Sbjct: 200 LLD--PDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFEIA 259

Query: 262 ESLIQERRRIHLENGS--IVGDFLDVMIQ-----------SGFSDEQISHLAVELLSAGT 321
              I+ER  +     S     D+LDV+++           S FS   I+ +  E+ +AGT
Sbjct: 260 GEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGT 319

Query: 322 DTTTTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPKLQYLQSCIKEALRL 381
           DTTT+T+EWAL ELL N   L+K Q EL+         L+E  +P L YL + I E LRL
Sbjct: 320 DTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLRL 379

Query: 382 HPPAPFLLPRTAPQACNLMG-YTIPKNALIFVNVWGIGRDPSVWEDCGSFHPDRF-ADCD 441
           HPP PFL+P  A   C++   YTIPK   + VNVW IGRDP  W D   F P+RF +D D
Sbjct: 380 HPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFISDPD 439

Query: 442 AGFNNGFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPKGGDLGHLGLEGR 483
           A    G D+ +LPFG GRRMCP L +A+  +PL +G++V  FDW    G +   + +  R
Sbjct: 440 ARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGER 499

[Close]

BLAST of Sed0004505 vs. TAIR 10
Match: AT3G52970.2 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 310.1 bits (793), Expect = 3.2e-84
Identity = 184/509 (36.15%), Postives = 270/509 (53.05%), Query Frame = 0


Query: 22  LIFILLKIFNPPSPNNLPPGPRPWPILGNLHQL-GRNAHISLTNLAKIHGPLVSLKLGSQ 81
           LI++    +       LPPGP PWP++GN+ QL G   H SLT L++ HGP+++L++GS 
Sbjct: 20  LIYVTCLFYTKRCRTRLPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSM 79

Query: 82  PLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNSSLVFSPHCNDHWKSLRSICR 141
             VV SS   A  + K  D  L+ R I +        ++ SL+ + +   +W+ LR +C 
Sbjct: 80  LTVVISSSEVAREIFKKHDAALAGRKIYEAMKG-GKSSDGSLITAQY-GAYWRMLRRLCT 139

Query: 142 ANLFNAKSIESQAGLRARKIDEMIK------------------------------DFVFT 201
              F  + +++ + +R+R +D+M++                               + F 
Sbjct: 140 TQFFVTRRLDAMSDVRSRCVDQMLRFVEEGGQNGMYYTINNIMTCIICTKTIDVGRYFFL 199

Query: 202 TVFNTISNLIFSRDLLGFCGDGFHGIKVPFK--KMMDLGLTPNLADLYPWLRRFDLQGLR 261
             FN I NL+FSRDLL    D   G +  +   K+M+    PN+AD +P LR  D QG+R
Sbjct: 200 MAFNLIGNLMFSRDLLD--PDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFLDPQGIR 259

Query: 262 RKARVYKNEVDSSWESLIQERRRIHLENGS--IVGDFLDVMIQ-----------SGFSDE 321
           RK + +  +        I+ER  +     S     D+LDV+++           S FS  
Sbjct: 260 RKTQFHVEKAFEIAGEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSR 319

Query: 322 QISHLAVELLSAGTDTTTTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPK 381
            I+ +  E+ +AGTDTTT+T+EWAL ELL N   L+K Q EL+         L+E  +P 
Sbjct: 320 DINVIVFEMFTAGTDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQKLQEEDLPN 379

Query: 382 LQYLQSCIKEALRLHPPAPFLLPRTAPQACNLMG-YTIPKNALIFVNVWGIGRDPSVWED 441
           L YL + I E LRLHPP PFL+P  A   C++   YTIPK   + VNVW IGRDP  W D
Sbjct: 380 LPYLSAVIMETLRLHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIGRDPKTWID 439

Query: 442 CGSFHPDRF-ADCDAGFNNGFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVC 483
              F P+RF +D DA    G D+ +LPFG GRRMCP L +A+  +PL +G++V  FDW  
Sbjct: 440 PIMFKPERFISDPDARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWAL 499

[Close]

BLAST of Sed0004505 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 302.0 bits (772), Expect = 8.8e-82
Identity = 188/510 (36.86%), Postives = 276/510 (54.12%), Query Frame = 0


Query: 7   LDILSFPLLLLLFLPLIFILLKIFNPPS------PNNLPPGPRPWPILGNLHQLGRNAHI 66
           +DI+S   LLLLF  ++   L      S         LPPGP   PI+GN+H +G++ H 
Sbjct: 1   MDIISGQALLLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHR 60

Query: 67  SLTNLAKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSLHNNS 126
           S   L+K +GP++SLKLGS   VV +SP AA  VL+T D++LSAR       +++ H ++
Sbjct: 61  SFAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAVRSIN-HQDA 120

Query: 127 SLVFSPHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEM---------------IK 186
           SLV+ P  +  W+ LR +    L + + IE+   LR  K+ E+               I 
Sbjct: 121 SLVWLPSSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDIS 180

Query: 187 DFVFTTVFNTISNLIFSRDLLGF-CGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRRFDL 246
              F T  N ISN++FS DL  +      +G++     +MD   TP+ A+ +P+LR  DL
Sbjct: 181 RVAFITTLNIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLDL 240

Query: 247 QG--------LRRKARVYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVM-IQSGFSDE 306
           QG          R  RV++  +D+       +     +     V + LD    +S  S  
Sbjct: 241 QGNVKTFKVCTERLVRVFRGFIDAKIAEKSSQNNPKDVSKNDFVDNLLDYKGDESELSIS 300

Query: 307 QISHLAVELLSAGTDTTTTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEESQIPK 366
            I HL +++ +AGTDT+++T+EWA+TELLKN   ++KAQ E+      Q   +EES I K
Sbjct: 301 DIEHLLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEID-CVIGQNGIVEESDISK 360

Query: 367 LQYLQSCIKEALRLHPPAPFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPSVWEDC 426
           L YLQ+ +KE  RLH P P L+PR A     ++G+ + K+  + VNVW IGRDPSVW++ 
Sbjct: 361 LPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNP 420

Query: 427 GSFHPDRFADCDAGFNNGFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFDWVCPK 486
             F P+RF   D     G D+   PFG GRR+CPG+ +A   V L+L +L++ FDW  PK
Sbjct: 421 SQFEPERFLGKDMDV-RGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPK 480

[Close]

BLAST of Sed0004505 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 295.4 bits (755), Expect = 8.2e-80
Identity = 187/515 (36.31%), Postives = 273/515 (53.01%), Query Frame = 0


Query: 7   LDILSFPLLLLLFLPLIFILLKIFNPPSPNN------LPPGPRPWPILGNLHQLGRNAHI 66
           +DI+    L  LF  ++   +  F    P +       PPGP   PI+GN+H +GRN H 
Sbjct: 1   MDIIFEQALFPLFCFVLSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHH 60

Query: 67  SLTNLAKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSL---- 126
           S  +L+K +GP++SLK GS   VV +SP AA  VL+T D++LS+R     TP  S+    
Sbjct: 61  SFADLSKTYGPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSIN 120

Query: 127 HNNSSLVFSPHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEM------------- 186
           H+  S+V+ P  +  W+ LR +    LF+ + IE+   LR  K+ E+             
Sbjct: 121 HDKVSVVWLPPSSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEA 180

Query: 187 --IKDFVFTTVFNTISNLIFSRDLLGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRR 246
             I    F T  N ISN++FS DL  +  +     +     +M+    P+ A+ +P+L  
Sbjct: 181 VDISRATFITALNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGF 240

Query: 247 FDLQGLRRKARVYKNEVDSSWESLIQER---RRIHLENGSIV--GDFLDVMIQSGFSDE- 306
            DLQG R+  +     +   +   I  +   + +   N   V   DF+DV++     DE 
Sbjct: 241 LDLQGNRKTLKACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEA 300

Query: 307 -----QISHLAVELLSAGTDTTTTTIEWALTELLKNRAALSKAQRELKAATTKQKPTLEE 366
                 I HL ++L  AGTDT ++T+EWA+ ELL+N   + KAQ E+      QK  +EE
Sbjct: 301 ELNTNDIVHLLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEID-CVIGQKGVVEE 360

Query: 367 SQIPKLQYLQSCIKEALRLHPPAPFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGRDPS 426
           S I  L YLQ+ +KE  RLHP AP L+PR A     ++G+ +PK+  +FVNVW IGRDP+
Sbjct: 361 SDISALPYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPN 420

Query: 427 VWEDCGSFHPDRFADCDAGFNNGFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVHCFD 486
           VWE+   F P+RF   D     G D+   PFG GRR+CPGL +A   VPL+L +L++ FD
Sbjct: 421 VWENSSRFKPERFLGKDIDL-RGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFD 480

[Close]

BLAST of Sed0004505 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 282.7 bits (722), Expect = 5.5e-76
Identity = 185/518 (35.71%), Postives = 276/518 (53.28%), Query Frame = 0


Query: 7   LDILSFPLLLLLFLPLIFILLKIFNPPSPNN------LPPGPRPWPILGNLHQLGRNAHI 66
           +DI+S   L LLF  +    L      S  +      LPPGP   PI+GN+HQ+G+N H 
Sbjct: 1   MDIISGQALFLLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHS 60

Query: 67  SLTNLAKIHGPLVSLKLGSQPLVVASSPVAAAAVLKTEDRLLSARYILQMTPNLSL---- 126
           S  +LAKI+GP++SLK G    VV +SP AA  VL+T D++LS R       N S+    
Sbjct: 61  SFADLAKIYGPIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGR-----KSNDSIRCFG 120

Query: 127 HNNSSLVFSPHCNDHWKSLRSICRANLFNAKSIESQAGLRARKIDEMI---------KDF 186
           H   S+++ P  +  W+ LR +    +F+ +  E+   LR +K+ E++         K+ 
Sbjct: 121 HEEVSVIWLPPSSARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEA 180

Query: 187 V------FTTVFNTISNLIFSRDLLGFCGDGFHGIKVPFKKMMDLGLTPNLADLYPWLRR 246
           V      +TTV N ISN++FS DL  +     +  +      M+    P+ A+ +P++  
Sbjct: 181 VDISRASYTTVLNIISNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGF 240

Query: 247 FDLQGLR--------RKARVYKNEVDSSWESLIQERRRIHLENGSIVGDFLDVMIQSGFS 306
            DLQG R        R  RV++  +D+     I E+   +        DFLD ++     
Sbjct: 241 LDLQGNRKAMRGLTERLFRVFRGFMDAK----IAEKSLGNYSKDVSNRDFLDSLLILNEG 300

Query: 307 DE------QISHLAVELLSAGTDTTTTTIEWALTELLKNRAALSKAQRELKAATTKQKPT 366
           DE       I HL +++ +AGTDT+++T+EWA+ ELL+N   + KAQ E+      Q   
Sbjct: 301 DEAELDNNDIEHLLLDMFTAGTDTSSSTLEWAMAELLRNPKTMVKAQAEMD-RVLGQNSV 360

Query: 367 LEESQIPKLQYLQSCIKEALRLHPPAPFLLPRTAPQACNLMGYTIPKNALIFVNVWGIGR 426
           ++ES I  L YLQ+ +KE  RLHP AP L+PR A     ++G+ +PK+  + VNVW IGR
Sbjct: 361 VQESDISGLPYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGR 420

Query: 427 DPSVWEDCGSFHPDRFADCDAGFNNGFDFGYLPFGGGRRMCPGLAMAAVQVPLILGTLVH 486
           DPSVWE+   F P+RF   D     G D+   PFGGGRR+CPGL +A   V L+L +L++
Sbjct: 421 DPSVWENPSQFEPERFMGKDIDV-KGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLY 480

The following BLAST results are available for this feature:

BLAST of Sed0004505 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031741123.1	2.7e-173	65.19	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus]	[more]
KAE8647950.1	5.9e-173	64.40	hypothetical protein Csa_000545 [Cucumis sativus]	[more]
XP_008467223.1	5.9e-173	63.53	PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis mel...	[more]
XP_022928791.1	1.0e-172	64.10	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata]	[more]
KAA0055170.1	1.0e-172	63.33	putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. maku...	[more]

BLAST of Sed0004505 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SP06	2.3e-103	43.18	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum...	[more]
O64899	2.0e-102	42.26	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif...	[more]
Q9FXW4	3.3e-102	43.32	Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3...	[more]
O64900	2.2e-101	40.93	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3...	[more]
A8CDR5	1.9e-97	41.41	Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1	[more]

BLAST of Sed0004505 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CT14	2.9e-173	63.53	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656...	[more]
A0A5A7UIU3	4.9e-173	63.33	Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma...	[more]
A0A6J1ELA6	4.9e-173	64.10	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O...	[more]
A0A6J1I4V7	1.5e-169	62.88	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=...	[more]
A0A6J1ER52	2.5e-169	62.35	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O...	[more]

BLAST of Sed0004505 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52970.1	7.7e-86	37.17	cytochrome P450, family 76, subfamily G, polypeptide 1	[more]
AT3G52970.2	3.2e-84	36.15	cytochrome P450, family 76, subfamily G, polypeptide 1	[more]
AT2G45560.1	8.8e-82	36.86	cytochrome P450, family 76, subfamily C, polypeptide 1	[more]
AT2G45570.1	8.2e-80	36.31	cytochrome P450, family 76, subfamily C, polypeptide 2	[more]
AT2G45550.1	5.5e-76	35.71	cytochrome P450, family 76, subfamily C, polypeptide 4	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 340..358 score: 34.95 coord: 66..85 score: 34.63 coord: 296..322 score: 24.47 coord: 428..451 score: 29.61 coord: 276..293 score: 31.04 coord: 381..405 score: 33.8 coord: 418..428 score: 54.73
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 428..439 score: 28.33 coord: 341..352 score: 40.76 coord: 419..428 score: 59.82 coord: 287..304 score: 43.18
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 39..457 e-value: 4.2E-84 score: 282.9
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 32..483 e-value: 2.5E-106 score: 358.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 39..485
None	No IPR available	PANTHER	PTHR47956:SF23	(S)-N-METHYLCOCLAURINE 3'-HYDROXYLASE ISOZYME 1-LIKE	coord: 14..483
None	No IPR available	PANTHER	PTHR47956	CYTOCHROME P450 71B11-RELATED	coord: 14..483
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 421..430

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0004505.1	Sed0004505.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen