Sed0004437 (gene) Chayote v1

Overview


Name	Sed0004437
Type	gene
Organism	Sechium edule (Chayote v1)
Description	aspartic proteinase PCS1
Location	LG02: 44554772 .. 44556692 (+)
RNA-Seq Expression	Sed0004437
Synteny	Sed0004437

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGGTAATTTATTGGTTTTTCTTTTTAATATTTGAAATTTATACTGTTGGATCTTCGCAGGAGAGAAGTTTCTGAAAATATTTTCAAAGATTATCAGAACACTCTGTTCTCAAAACTTCCAAAGATTTCTTCACTCTGTTTTTCCAACTGCTCCAACTGTTTACCACTCCCAATTTTCCAACAAACAAAAACAAAACAAAACAAAAATGAGGGATTATTGTATAAATTTCAATCCCTCAAACTACAAATCTCTCAAATCTTCTTTCCTTTTCTTCTTCACTTCTCTGATTTTGGTCTTTCAAAACCCAAGACTCTGTTTCTCGTTAAACCCAGCCCTGCTTTTGCCTCTCAAAACCCAAGTAATTCCGCCAGAATCTTTCCGGCGATCTCCGGACAAGCTCCCTTTCCGGCACAACATCAGCCTTACCGTCTCATTGACGGTCGGATCGCCGCCGCAGAATGTCACAATGGTCATCGACACCGGCAGCGAACTCTCATGGCTCCGCTGCAACAGATCACAAAATTCTTCAGCTTCTTCTTCGACATTCCACCCGGTCCGGTCCACCTCGTACGCTCCGATCCCATGTTCCTCGCCCACCTGCACGAACCAAACCCGGGATTTTCCGATTCCGGCATCGTGTGATTCCGATCAGCTCTGTCACGCCACTCTCTCCTACGCCGACGCCTCTTCTTCAGAAGGAAATCTCGCCGCCGATACGTTTTATTTCGGAAATTCCGGAATTCCCAATGTGATTTTCGGTTGTATGGATTCGATTTTCAGTTCCAACAATGAAGAGGATTCCAAGAACACCGGATTAATGGGTATGAATCGTGGATCTTTATCTTTCGTTTCTCAAATGGGTTTCCCCAAATTCTCATATTGCATATCGGAATATGATTTTTCTGGTTTGTTACTTCTCGGAGATGCCAATTTCTCCTGGTTAGCTCCGTTGAATTACACTCCACTCATCGAAATGTCGACGCCGTTGCCGTATTTCGATCGAGTCGCTTACACGGTTCAGCTCGAAGGGATCAAAGTTTCGCACAAACTAATTCCGATACCGGAATCGGTTTTCGAACCGGATCACACAGGCGCCGGTCAGACAATGGTCGATTCCGGCACCCAGTTCACTTTCCTTCTCGGACCGGCTTACACCGCGCTCCGAGACGAGTTCCTGAACAAAACCGCCGGTTCGCTTCGGGTTTTCGAGGATCCGAATTTCGTTTTCCAAGGCGCAATGGACCTTTGCTACCGGGTTCCGATGACCCAAACCCGGCTCCCGCCGCTGCCGTCGGTGACGCTGGTGTTTAGCGGCGCCGAGATGACGGTCACCGGCGACCGGATTCTGTACCGGGTGCCCGGCGAAACAAGAGGGAGCGATTCGATTCATTGCTTCACGTTCGGAAATTCGGATCTTCTCGGCGTTGAAGCGTTCGTGATCGGCCATCTTCATCAACAAAACGTGTGGATGGAATTCGATCTCAAGAAATCTCGGATCGGGTTGGCGAAGATTCGGTGCGATTTGGCGGGTCAGAAATTGGGAATGGGCCTGTGAAGTGACCCGGGCCCATTTGATTGAAGCCTTTTAACTTAGCACGTGATGTTGCTTAAAATCTTACGGGTCTAGTTGTGTAGTTGAGGTCCATGCCATTATGATGGTCCTTCAGGTTTTAAATACCGTCCGTATGTGGGTCCCTTACCATCTTGTATATTTTTAAATATAAATTTATACAAAAGTGAGTTTGATCCGACGGGTGTAGTGAGTTTGAGTGAGCTGTGATGTCGCCACGTGTACGAAGTAAGAATAATTTTTCTTTTATTTTCTTCTTTTCCGCTCACCTTGTACAATACTTCACGAGTTTTAATGTAATGAAAATATATACATATATTATGCAAAATGATTTTTTTTTTTTTAACGAA

mRNA sequence

GGGGTAATTTATTGGTTTTTCTTTTTAATATTTGAAATTTATACTGTTGGATCTTCGCAGGAGAGAAGTTTCTGAAAATATTTTCAAAGATTATCAGAACACTCTGTTCTCAAAACTTCCAAAGATTTCTTCACTCTGTTTTTCCAACTGCTCCAACTGTTTACCACTCCCAATTTTCCAACAAACAAAAACAAAACAAAACAAAAATGAGGGATTATTGTATAAATTTCAATCCCTCAAACTACAAATCTCTCAAATCTTCTTTCCTTTTCTTCTTCACTTCTCTGATTTTGGTCTTTCAAAACCCAAGACTCTGTTTCTCGTTAAACCCAGCCCTGCTTTTGCCTCTCAAAACCCAAGTAATTCCGCCAGAATCTTTCCGGCGATCTCCGGACAAGCTCCCTTTCCGGCACAACATCAGCCTTACCGTCTCATTGACGGTCGGATCGCCGCCGCAGAATGTCACAATGGTCATCGACACCGGCAGCGAACTCTCATGGCTCCGCTGCAACAGATCACAAAATTCTTCAGCTTCTTCTTCGACATTCCACCCGGTCCGGTCCACCTCGTACGCTCCGATCCCATGTTCCTCGCCCACCTGCACGAACCAAACCCGGGATTTTCCGATTCCGGCATCGTGTGATTCCGATCAGCTCTGTCACGCCACTCTCTCCTACGCCGACGCCTCTTCTTCAGAAGGAAATCTCGCCGCCGATACGTTTTATTTCGGAAATTCCGGAATTCCCAATGTGATTTTCGGTTGTATGGATTCGATTTTCAGTTCCAACAATGAAGAGGATTCCAAGAACACCGGATTAATGGGTATGAATCGTGGATCTTTATCTTTCGTTTCTCAAATGGGTTTCCCCAAATTCTCATATTGCATATCGGAATATGATTTTTCTGGTTTGTTACTTCTCGGAGATGCCAATTTCTCCTGGTTAGCTCCGTTGAATTACACTCCACTCATCGAAATGTCGACGCCGTTGCCGTATTTCGATCGAGTCGCTTACACGGTTCAGCTCGAAGGGATCAAAGTTTCGCACAAACTAATTCCGATACCGGAATCGGTTTTCGAACCGGATCACACAGGCGCCGGTCAGACAATGGTCGATTCCGGCACCCAGTTCACTTTCCTTCTCGGACCGGCTTACACCGCGCTCCGAGACGAGTTCCTGAACAAAACCGCCGGTTCGCTTCGGGTTTTCGAGGATCCGAATTTCGTTTTCCAAGGCGCAATGGACCTTTGCTACCGGGTTCCGATGACCCAAACCCGGCTCCCGCCGCTGCCGTCGGTGACGCTGGTGTTTAGCGGCGCCGAGATGACGGTCACCGGCGACCGGATTCTGTACCGGGTGCCCGGCGAAACAAGAGGGAGCGATTCGATTCATTGCTTCACGTTCGGAAATTCGGATCTTCTCGGCGTTGAAGCGTTCGTGATCGGCCATCTTCATCAACAAAACGTGTGGATGGAATTCGATCTCAAGAAATCTCGGATCGGGTTGGCGAAGATTCGGTGCGATTTGGCGGGTCAGAAATTGGGAATGGGCCTGTGAAGTGACCCGGGCCCATTTGATTGAAGCCTTTTAACTTAGCACGTGATGTTGCTTAAAATCTTACGGGTCTAGTTGTGTAGTTGAGGTCCATGCCATTATGATGGTCCTTCAGGTTTTAAATACCGTCCGTATGTGGGTCCCTTACCATCTTGTATATTTTTAAATATAAATTTATACAAAAGTGAGTTTGATCCGACGGGTGTAGTGAGTTTGAGTGAGCTGTGATGTCGCCACGTGTACGAAGTAAGAATAATTTTTCTTTTATTTTCTTCTTTTCCGCTCACCTTGTACAATACTTCACGAGTTTTAATGTAATGAAAATATATACATATATTATGCAAAATGATTTTTTTTTTTTTAACGAA

Coding sequence (CDS)

ATGAGGGATTATTGTATAAATTTCAATCCCTCAAACTACAAATCTCTCAAATCTTCTTTCCTTTTCTTCTTCACTTCTCTGATTTTGGTCTTTCAAAACCCAAGACTCTGTTTCTCGTTAAACCCAGCCCTGCTTTTGCCTCTCAAAACCCAAGTAATTCCGCCAGAATCTTTCCGGCGATCTCCGGACAAGCTCCCTTTCCGGCACAACATCAGCCTTACCGTCTCATTGACGGTCGGATCGCCGCCGCAGAATGTCACAATGGTCATCGACACCGGCAGCGAACTCTCATGGCTCCGCTGCAACAGATCACAAAATTCTTCAGCTTCTTCTTCGACATTCCACCCGGTCCGGTCCACCTCGTACGCTCCGATCCCATGTTCCTCGCCCACCTGCACGAACCAAACCCGGGATTTTCCGATTCCGGCATCGTGTGATTCCGATCAGCTCTGTCACGCCACTCTCTCCTACGCCGACGCCTCTTCTTCAGAAGGAAATCTCGCCGCCGATACGTTTTATTTCGGAAATTCCGGAATTCCCAATGTGATTTTCGGTTGTATGGATTCGATTTTCAGTTCCAACAATGAAGAGGATTCCAAGAACACCGGATTAATGGGTATGAATCGTGGATCTTTATCTTTCGTTTCTCAAATGGGTTTCCCCAAATTCTCATATTGCATATCGGAATATGATTTTTCTGGTTTGTTACTTCTCGGAGATGCCAATTTCTCCTGGTTAGCTCCGTTGAATTACACTCCACTCATCGAAATGTCGACGCCGTTGCCGTATTTCGATCGAGTCGCTTACACGGTTCAGCTCGAAGGGATCAAAGTTTCGCACAAACTAATTCCGATACCGGAATCGGTTTTCGAACCGGATCACACAGGCGCCGGTCAGACAATGGTCGATTCCGGCACCCAGTTCACTTTCCTTCTCGGACCGGCTTACACCGCGCTCCGAGACGAGTTCCTGAACAAAACCGCCGGTTCGCTTCGGGTTTTCGAGGATCCGAATTTCGTTTTCCAAGGCGCAATGGACCTTTGCTACCGGGTTCCGATGACCCAAACCCGGCTCCCGCCGCTGCCGTCGGTGACGCTGGTGTTTAGCGGCGCCGAGATGACGGTCACCGGCGACCGGATTCTGTACCGGGTGCCCGGCGAAACAAGAGGGAGCGATTCGATTCATTGCTTCACGTTCGGAAATTCGGATCTTCTCGGCGTTGAAGCGTTCGTGATCGGCCATCTTCATCAACAAAACGTGTGGATGGAATTCGATCTCAAGAAATCTCGGATCGGGTTGGCGAAGATTCGGTGCGATTTGGCGGGTCAGAAATTGGGAATGGGCCTGTGA

Protein sequence

MRDYCINFNPSNYKSLKSSFLFFFTSLILVFQNPRLCFSLNPALLLPLKTQVIPPESFRRSPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQNSSASSSTFHPVRSTSYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYFGNSGIPNVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLPPLPSVTLVFSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSRIGLAKIRCDLAGQKLGMGL

Homology

[Close]

BLAST of Sed0004437 vs. NCBI nr
Match: XP_038905417.1 (aspartic proteinase PCS1 [Benincasa hispida])

HSP 1 Score: 827.0 bits (2135), Expect = 7.7e-236
Identity = 404/449 (89.98%), Postives = 423/449 (94.21%), Query Frame = 0


Query: 1   MRDYCINFNPSNYKSLKSSFLFFFTSLILVFQNPRLCFSLNPALLLPLKTQVIPPESFRR 60
           MRDYC  FN S+ K LK  F+FFF +L  VFQN +LC SLNPAL+LPLKTQVIPPES RR
Sbjct: 1   MRDYCFAFNSSSNKFLKYCFIFFFCTLFCVFQNSKLCSSLNPALVLPLKTQVIPPESVRR 60

Query: 61  SPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQNSSASSSTFHPVRST 120
           SPDKLPFRHNISLTVSLTVG+PPQNVTMVIDTGSELSWL CNRSQNSS+SSSTF+PVRS+
Sbjct: 61  SPDKLPFRHNISLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVRSS 120

Query: 121 SYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYFGNSGIP 180
           SY PIPCSS TCT+QTRDFPI  SCDS+Q CHATLSYADASSSEGNLA DTFY GNSGIP
Sbjct: 121 SYTPIPCSSSTCTDQTRDFPIRPSCDSNQFCHATLSYADASSSEGNLATDTFYIGNSGIP 180

Query: 181 NVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240
           NV+FGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD
Sbjct: 181 NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240

Query: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPDHTGAGQT 300
           ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKL+PIPESVFEPDHTGAGQT
Sbjct: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQT 300

Query: 301 MVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLPP 360
           MVDSGTQFTFLLGPAY ALRDEF+N+TAGS+RV EDPNFVFQGAMDLCYRVP+ QTRLPP
Sbjct: 301 MVDSGTQFTFLLGPAYAALRDEFVNQTAGSIRVLEDPNFVFQGAMDLCYRVPINQTRLPP 360

Query: 361 LPSVTLVFSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQNV 420
           LPSVTLVF GAEMTVTGDRILYRVPGE RG+DSIHCFTFGNSDLLGVEAFVIGHLHQQNV
Sbjct: 361 LPSVTLVFRGAEMTVTGDRILYRVPGERRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNV 420

Query: 421 WMEFDLKKSRIGLAKIRCDLAGQKLGMGL 450
           WMEFDLKKSRIGLA+IRCDLAGQKLGMGL
Sbjct: 421 WMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Sed0004437 vs. NCBI nr
Match: XP_022983486.1 (aspartic proteinase PCS1 [Cucurbita maxima])

HSP 1 Score: 826.2 bits (2133), Expect = 1.3e-235
Identity = 407/449 (90.65%), Postives = 421/449 (93.76%), Query Frame = 0


Query: 1   MRDYCINFNPSNYKSLKSSFLFFFTSLILVFQNPRLCFSLNPALLLPLKTQVIPPESFRR 60
           MRDYCI FN SN+K LKS F FF   L  VFQN  LC SLNPALLLPLKTQVIPPES RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSFFPFFLCILFSVFQNLILCSSLNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQNSSASSSTFHPVRST 120
           SPDKLPFRHN+SLTVSLTVG+PPQNVTMVIDTGSELSWL CNRSQNSS+SSSTF+P  S+
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPAGSS 120

Query: 121 SYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYFGNSGIP 180
           SY PIPCSS TCT+QTRDFPIPASCDS+ LCHATLSYADASSSEG LA DTFY GNSGI 
Sbjct: 121 SYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGIS 180

Query: 181 NVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240
           NV+FGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD
Sbjct: 181 NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240

Query: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPDHTGAGQT 300
           ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKL+PIPESVFEPDHTGAGQT
Sbjct: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQT 300

Query: 301 MVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLPP 360
           MVDSGTQFTFLLGPAYTALRDEFLN+TAGS+RVFED NFVFQGAMDLCYRVPM QTRLPP
Sbjct: 301 MVDSGTQFTFLLGPAYTALRDEFLNRTAGSIRVFEDSNFVFQGAMDLCYRVPMNQTRLPP 360

Query: 361 LPSVTLVFSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQNV 420
           LPSVTLVF GAEMTVTGDRILYRVPGE RG+DSIHCFTFGNSDLLGVEAFVIGHLHQQNV
Sbjct: 361 LPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNV 420

Query: 421 WMEFDLKKSRIGLAKIRCDLAGQKLGMGL 450
           WMEFDLKKSRIGLA+IRCDLAGQKLGMGL
Sbjct: 421 WMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Sed0004437 vs. NCBI nr
Match: XP_023528618.1 (aspartic proteinase PCS1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 818.5 bits (2113), Expect = 2.8e-233
Identity = 405/450 (90.00%), Postives = 420/450 (93.33%), Query Frame = 0


Query: 1   MRDYCINFNPSNYKSLKSSFLFFFTSLILVFQNPRLCFSLNPALLLPLKTQVIPPESFRR 60
           MRDYCI FN SN+K LKS F FF  +L  VFQN  LC S NPALLLPLKTQVIPPES RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQN-SSASSSTFHPVRS 120
           SPDKLPFRHN+SLTVSLTVG+PPQNVTMVIDTGSELSWL CNRSQN SS+SSSTF+P  S
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120

Query: 121 TSYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYFGNSGI 180
           +SY PIPCSS TCT+QTRDFPIPASCDS+ LCHATLSYADASSSEG LA DTFY GNSGI
Sbjct: 121 SSYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 PNVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
            NV+FGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEM+TPLPYFDRVAYTVQLEGIKVSHKL+PIPESVFEPDHTGAGQ
Sbjct: 241 DANFSWLAPLNYTPLIEMTTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RVFED NFVFQGAMDLCYRVPM QTRLP
Sbjct: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360

Query: 361 PLPSVTLVFSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVF GAEMTVTGDRILYRVPGE RG+DSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420

Query: 421 VWMEFDLKKSRIGLAKIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLA+IRCDLAGQKLGMGL
Sbjct: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450

[Close]

BLAST of Sed0004437 vs. NCBI nr
Match: XP_023528617.1 (aspartic proteinase PCS1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 818.5 bits (2113), Expect = 2.8e-233
Identity = 405/450 (90.00%), Postives = 420/450 (93.33%), Query Frame = 0


Query: 1   MRDYCINFNPSNYKSLKSSFLFFFTSLILVFQNPRLCFSLNPALLLPLKTQVIPPESFRR 60
           MRDYCI FN SN+K LKS F FF  +L  VFQN  LC S NPALLLPLKTQVIPPES RR
Sbjct: 55  MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 114

Query: 61  SPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQN-SSASSSTFHPVRS 120
           SPDKLPFRHN+SLTVSLTVG+PPQNVTMVIDTGSELSWL CNRSQN SS+SSSTF+P  S
Sbjct: 115 SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 174

Query: 121 TSYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYFGNSGI 180
           +SY PIPCSS TCT+QTRDFPIPASCDS+ LCHATLSYADASSSEG LA DTFY GNSGI
Sbjct: 175 SSYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 234

Query: 181 PNVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
            NV+FGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 235 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 294

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEM+TPLPYFDRVAYTVQLEGIKVSHKL+PIPESVFEPDHTGAGQ
Sbjct: 295 DANFSWLAPLNYTPLIEMTTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 354

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RVFED NFVFQGAMDLCYRVPM QTRLP
Sbjct: 355 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 414

Query: 361 PLPSVTLVFSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVF GAEMTVTGDRILYRVPGE RG+DSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 415 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 474

Query: 421 VWMEFDLKKSRIGLAKIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLA+IRCDLAGQKLGMGL
Sbjct: 475 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 504

[Close]

BLAST of Sed0004437 vs. NCBI nr
Match: XP_022935018.1 (aspartic proteinase PCS1 [Cucurbita moschata] >KAG6580532.1 Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 818.1 bits (2112), Expect = 3.6e-233
Identity = 405/450 (90.00%), Postives = 420/450 (93.33%), Query Frame = 0


Query: 1   MRDYCINFNPSNYKSLKSSFLFFFTSLILVFQNPRLCFSLNPALLLPLKTQVIPPESFRR 60
           MRDYCI FN SN+K LKS F FF  +L  VFQN  LC S NPALLLPLKTQVIPPES RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQN-SSASSSTFHPVRS 120
           SPDKLPFRHN+SLTVSLTVG+PPQNVTMVIDTGSELSWL CNRSQN SS+SSSTF+P  S
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120

Query: 121 TSYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYFGNSGI 180
           +SY PIPCSS TCT++TRDFPIPASCDS+ LCHATLSYADASSSEG LA DTFY GNSGI
Sbjct: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 PNVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
            NV+FGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKL+PIPESVFEPDHTGAGQ
Sbjct: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RVFED NFVFQGAMDLCYRVPM QTRLP
Sbjct: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360

Query: 361 PLPSVTLVFSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVF GAEMTVTGDRILYRVPGE RG+DSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420

Query: 421 VWMEFDLKKSRIGLAKIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLA+IRCDLAGQKLGMGL
Sbjct: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450

[Close]

BLAST of Sed0004437 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 1.8e-166
Identity = 289/415 (69.64%), Postives = 346/415 (83.37%), Query Frame = 0


Query: 39  SLNPALLLPLKTQVIPPESFRRSPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSW 98
           S +  L+LPLKT++ P +   R  DKL F HN++LTV+LTVG+PPQN++MVIDTGSELSW
Sbjct: 41  SSSQTLVLPLKTRITPTD--HRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELSW 100

Query: 99  LRCNRSQNSSASSSTFHPVRSTSYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYA 158
           LRCNRS N +  ++ F P RS+SY+PIPCSSPTC  +TRDF IPASCDSD+LCHATLSYA
Sbjct: 101 LRCNRSSNPNPVNN-FDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSYA 160

Query: 159 DASSSEGNLAADTFYFGNS-GIPNVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQ 218
           DASSSEGNLAA+ F+FGNS    N+IFGCM S+  S+ EED+K TGL+GMNRGSLSF+SQ
Sbjct: 161 DASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQ 220

Query: 219 MGFPKFSYCIS-EYDFSGLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGI 278
           MGFPKFSYCIS   DF G LLLGD+NF+WL PLNYTPLI +STPLPYFDRVAYTVQL GI
Sbjct: 221 MGFPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGI 280

Query: 279 KVSHKLIPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFED 338
           KV+ KL+PIP+SV  PDHTGAGQTMVDSGTQFTFLLGP YTALR  FLN+T G L V+ED
Sbjct: 281 KVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYED 340

Query: 339 PNFVFQGAMDLCYRVPMTQTR---LPPLPSVTLVFSGAEMTVTGDRILYRVPGETRGSDS 398
           P+FVFQG MDLCYR+   + R   L  LP+V+LVF GAE+ V+G  +LYRVP  T G+DS
Sbjct: 341 PDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGNDS 400

Query: 399 IHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSRIGLAKIRCDLAGQKLGMG 449
           ++CFTFGNSDL+G+EA+VIGH HQQN+W+EFDL++SRIGLA + CD++GQ+LG+G
Sbjct: 401 VYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

[Close]

BLAST of Sed0004437 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.6e-37
Identity = 116/370 (31.35%), Postives = 187/370 (50.54%), Query Frame = 0


Query: 75  VSLTVGSPPQNVTMVIDTGSELSWLRCNR-SQNSSASSSTFHPVRSTSYAPIPCSSPTCT 134
           ++L++G+P Q  + ++DTGS+L W +C   +Q  + S+  F+P  S+S++ +PCSS  C 
Sbjct: 97  MNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLC- 156

Query: 135 NQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYFGNSGIPNVIFGCMDSIFSS 194
            Q    P   +C S+  C  T  Y D S ++G++  +T  FG+  IPN+ FGC       
Sbjct: 157 -QALSSP---TC-SNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGC-----GE 216

Query: 195 NNE--EDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFS--GLLLLGDANFSWLAPL 254
           NN+        GL+GM RG LS  SQ+   KFSYC++    S    LLLG    S  A  
Sbjct: 217 NNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGS 276

Query: 255 NYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFE-PDHTGAGQTMVDSGTQF 314
             T LI+ S+ +P F    Y + L G+ V    +PI  S F    + G G  ++DSGT  
Sbjct: 277 PNTTLIQ-SSQIPTF----YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTL 336

Query: 315 TFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLPPLPSVTLVF 374
           T+ +  AY ++R EF+++   +L V    +  F    DLC++ P   + L  +P+  + F
Sbjct: 337 TYFVNNAYQSVRQEFISQI--NLPVVNGSSSGF----DLCFQTPSDPSNL-QIPTFVMHF 396

Query: 375 SGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKK 434
            G ++ +  +            S+ + C   G+S   G+  F  G++ QQN+ + +D   
Sbjct: 397 DGGDLELPSENYF------ISPSNGLICLAMGSSS-QGMSIF--GNIQQQNMLVVYDTGN 434

Query: 435 SRIGLAKIRC 439
           S +  A  +C
Sbjct: 457 SVVSFASAQC 434

[Close]

BLAST of Sed0004437 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 4.2e-35
Identity = 102/367 (27.79%), Postives = 176/367 (47.96%), Query Frame = 0


Query: 75  VSLTVGSPPQNVTMVIDTGSELSWLRCNR-SQNSSASSSTFHPVRSTSYAPIPCSSPTCT 134
           +++ +G+P  + + ++DTGS+L W +C   +Q  S  +  F+P  S+S++ +PC S  C 
Sbjct: 98  MNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQ 157

Query: 135 NQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYFGNSGIPNVIFGCMDSIFSS 194
           +      +P+   ++  C  T  Y D S+++G +A +TF F  S +PN+ FGC +    +
Sbjct: 158 D------LPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGCGE---DN 217

Query: 195 NNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFS--GLLLLGDANFSWLAPLNY 254
                    GL+GM  G LS  SQ+G  +FSYC++ Y  S    L LG A          
Sbjct: 218 QGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPS 277

Query: 255 TPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPDHTGAGQTMVDSGTQFTFL 314
           T LI  S    Y     Y + L+GI V    + IP S F+    G G  ++DSGT  T+L
Sbjct: 278 TTLIHSSLNPTY-----YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYL 337

Query: 315 LGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLPPLPSVTLVFSGA 374
              AY A+   F ++   +L   ++ +      +  C++ P   + +  +P +++ F G 
Sbjct: 338 PQDAYNAVAQAFTDQI--NLPTVDESS----SGLSTCFQQPSDGSTV-QVPEISMQFDGG 397

Query: 375 EMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSRI 434
            + +    IL         ++ + C   G+S  LG+  F  G++ QQ   + +DL+   +
Sbjct: 398 VLNLGEQNIL------ISPAEGVICLAMGSSSQLGISIF--GNIQQQETQVLYDLQNLAV 435

Query: 435 GLAKIRC 439
                +C
Sbjct: 458 SFVPTQC 435

[Close]

BLAST of Sed0004437 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 4.7e-34
Identity = 121/371 (32.61%), Postives = 182/371 (49.06%), Query Frame = 0


Query: 77  LTVGSPPQNVTMVIDTGSELSWLRCNRSQNS-SASSSTFHPVRSTSYAPIPCSSPTCTNQ 136
           L VG+P + V MV+DTGS++ WL+C   +   S S   F P +S +YA IPCSSP C   
Sbjct: 146 LGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRL 205

Query: 137 TRDFPIPASCDS-DQLCHATLSYADASSSEGNLAADTFYFGNSGIPNVIFGCMDSIFSSN 196
                  A C++  + C   +SY D S + G+ + +T  F  + +  V  GC       N
Sbjct: 206 D-----SAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGC----GHDN 265

Query: 197 NEEDSKNTGLMGMNRGSLSFVSQMGF---PKFSYCISEYDFS---GLLLLGDANFSWLAP 256
                   GL+G+ +G LSF  Q G     KFSYC+ +   S     ++ G+A  S +A 
Sbjct: 266 EGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIA- 325

Query: 257 LNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIP-IPESVFEPDHTGAGQTMVDSGTQ 316
             +TPL+      P  D   Y V L GI V    +P +  S+F+ D  G G  ++DSGT 
Sbjct: 326 -RFTPLLSN----PKLD-TFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTS 385

Query: 317 FTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLPPLPSVTLV 376
            T L+ PAY A+RD F     G+  +   P+F      D C+   ++      +P+V L 
Sbjct: 386 VTRLIRPAYIAMRDAF---RVGAKTLKRAPDF---SLFDTCF--DLSNMNEVKVPTVVLH 445

Query: 377 FSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLK 436
           F GA++++      Y +P +T G     CF F  + + G+   +IG++ QQ   + +DL 
Sbjct: 446 FRGADVSLPATN--YLIPVDTNGK---FCFAFAGT-MGGLS--IIGNIQQQGFRVVYDLA 484

Query: 437 KSRIGLAKIRC 439
            SR+G A   C
Sbjct: 506 SSRVGFAPGGC 484

[Close]

BLAST of Sed0004437 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 2.0e-32
Identity = 121/385 (31.43%), Postives = 178/385 (46.23%), Query Frame = 0


Query: 72  SLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQNSSASSSTFHPVRSTSYAPIPCSSPT 131
           S  V   +GSP Q + + +DT ++ +W  C+    +  SSS F P  S+SYA +PCSS  
Sbjct: 78  SYVVRAGLGSPSQQLLLALDTSADATWAHCSPC-GTCPSSSLFAPANSSSYASLPCSSSW 137

Query: 132 C-TNQTRDFPIPASCDSDQLCHATL-------SYADASSSEGNLAADTFYFGNSGIPNVI 191
           C   Q +  P P          ATL        +ADA S +  LA+DT   G   IPN  
Sbjct: 138 CPLFQGQACPAPQGGGDAAPPPATLPTCAFSKPFADA-SFQAALASDTLRLGKDAIPNYT 197

Query: 192 FGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGF---PKFSYCISEYD---FSGLLL 251
           FGC+ S+  +    +    GL+G+ RG ++ +SQ G      FSYC+  Y    FSG L 
Sbjct: 198 FGCVSSV--TGPTTNMPRQGLLGLGRGPMALLSQAGSLYNGVFSYCLPSYRSYYFSGSLR 257

Query: 252 LGDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPD-HTG 311
           LG A       + YTP++      P+   + Y V + G+ V H  + +P   F  D  TG
Sbjct: 258 LG-AGGGQPRSVRYTPMLRN----PHRSSL-YYVNVTGLSVGHAWVKVPAGSFAFDAATG 317

Query: 312 AGQTMVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQT 371
           AG T+VDSGT  T    P Y ALR+EF  + A          +   GA D C+     + 
Sbjct: 318 AG-TVVDSGTVITRWTAPVYAALREEFRRQVAA------PSGYTSLGAFDTCFNT--DEV 377

Query: 372 RLPPLPSVTL-VFSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNS-DLLGVEAFVIGH 431
                P+VT+ +  G ++ +  +  L         +  + C     +   +     VI +
Sbjct: 378 AAGGAPAVTVHMDGGVDLALPMENTLIH-----SSATPLACLAMAEAPQNVNSVVNVIAN 437

Query: 432 LHQQNVWMEFDLKKSRIGLAKIRCD 440
           L QQN+ + FD+  SR+G AK  C+
Sbjct: 438 LQQQNIRVVFDVANSRVGFAKESCN 438

[Close]

BLAST of Sed0004437 vs. ExPASy TrEMBL
Match: A0A6J1J2F5 (aspartic proteinase PCS1 OS=Cucurbita maxima OX=3661 GN=LOC111482077 PE=3 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 6.4e-236
Identity = 407/449 (90.65%), Postives = 421/449 (93.76%), Query Frame = 0


Query: 1   MRDYCINFNPSNYKSLKSSFLFFFTSLILVFQNPRLCFSLNPALLLPLKTQVIPPESFRR 60
           MRDYCI FN SN+K LKS F FF   L  VFQN  LC SLNPALLLPLKTQVIPPES RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSFFPFFLCILFSVFQNLILCSSLNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQNSSASSSTFHPVRST 120
           SPDKLPFRHN+SLTVSLTVG+PPQNVTMVIDTGSELSWL CNRSQNSS+SSSTF+P  S+
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPAGSS 120

Query: 121 SYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYFGNSGIP 180
           SY PIPCSS TCT+QTRDFPIPASCDS+ LCHATLSYADASSSEG LA DTFY GNSGI 
Sbjct: 121 SYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGIS 180

Query: 181 NVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240
           NV+FGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD
Sbjct: 181 NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240

Query: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPDHTGAGQT 300
           ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKL+PIPESVFEPDHTGAGQT
Sbjct: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQT 300

Query: 301 MVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLPP 360
           MVDSGTQFTFLLGPAYTALRDEFLN+TAGS+RVFED NFVFQGAMDLCYRVPM QTRLPP
Sbjct: 301 MVDSGTQFTFLLGPAYTALRDEFLNRTAGSIRVFEDSNFVFQGAMDLCYRVPMNQTRLPP 360

Query: 361 LPSVTLVFSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQNV 420
           LPSVTLVF GAEMTVTGDRILYRVPGE RG+DSIHCFTFGNSDLLGVEAFVIGHLHQQNV
Sbjct: 361 LPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNV 420

Query: 421 WMEFDLKKSRIGLAKIRCDLAGQKLGMGL 450
           WMEFDLKKSRIGLA+IRCDLAGQKLGMGL
Sbjct: 421 WMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Sed0004437 vs. ExPASy TrEMBL
Match: A0A6J1F9C1 (aspartic proteinase PCS1 OS=Cucurbita moschata OX=3662 GN=LOC111442007 PE=3 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 1.7e-233
Identity = 405/450 (90.00%), Postives = 420/450 (93.33%), Query Frame = 0


Query: 1   MRDYCINFNPSNYKSLKSSFLFFFTSLILVFQNPRLCFSLNPALLLPLKTQVIPPESFRR 60
           MRDYCI FN SN+K LKS F FF  +L  VFQN  LC S NPALLLPLKTQVIPPES RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQN-SSASSSTFHPVRS 120
           SPDKLPFRHN+SLTVSLTVG+PPQNVTMVIDTGSELSWL CNRSQN SS+SSSTF+P  S
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120

Query: 121 TSYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYFGNSGI 180
           +SY PIPCSS TCT++TRDFPIPASCDS+ LCHATLSYADASSSEG LA DTFY GNSGI
Sbjct: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 PNVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
            NV+FGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKL+PIPESVFEPDHTGAGQ
Sbjct: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RVFED NFVFQGAMDLCYRVPM QTRLP
Sbjct: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360

Query: 361 PLPSVTLVFSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVF GAEMTVTGDRILYRVPGE RG+DSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420

Query: 421 VWMEFDLKKSRIGLAKIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLA+IRCDLAGQKLGMGL
Sbjct: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450

[Close]

BLAST of Sed0004437 vs. ExPASy TrEMBL
Match: A0A6J1F3C7 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111441767 PE=3 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 1.7e-233
Identity = 405/450 (90.00%), Postives = 420/450 (93.33%), Query Frame = 0


Query: 1   MRDYCINFNPSNYKSLKSSFLFFFTSLILVFQNPRLCFSLNPALLLPLKTQVIPPESFRR 60
           MRDYCI FN SN+K LKS F FF  +L  VFQN  LC S NPALLLPLKTQVIPPES RR
Sbjct: 186 MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 245

Query: 61  SPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQN-SSASSSTFHPVRS 120
           SPDKLPFRHN+SLTVSLTVG+PPQNVTMVIDTGSELSWL CNRSQN SS+SSSTF+P  S
Sbjct: 246 SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 305

Query: 121 TSYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYFGNSGI 180
           +SY PIPCSS TCT++TRDFPIPASCDS+ LCHATLSYADASSSEG LA DTFY GNSGI
Sbjct: 306 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 365

Query: 181 PNVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
            NV+FGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 366 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 425

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKL+PIPESVFEPDHTGAGQ
Sbjct: 426 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 485

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RVFED NFVFQGAMDLCYRVPM QTRLP
Sbjct: 486 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 545

Query: 361 PLPSVTLVFSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420
           PLPSVTLVF GAEMTVTGDRILYRVPGE RG+DSIHCFTFGNSDLLGVEAFVIGHLHQQN
Sbjct: 546 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 605

Query: 421 VWMEFDLKKSRIGLAKIRCDLAGQKLGMGL 450
           VWMEFDLKKSRIGLA+IRCDLAGQKLGMGL
Sbjct: 606 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 635

[Close]

BLAST of Sed0004437 vs. ExPASy TrEMBL
Match: A0A0A0LAI7 (Aspartic proteinase nepenthesin-2 OS=Cucumis sativus OX=3659 GN=Csa_3G734110 PE=3 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 2.5e-232
Identity = 401/449 (89.31%), Postives = 420/449 (93.54%), Query Frame = 0


Query: 1   MRDYCINFNPSNYKSLKSSFLFFFTSLILVFQNPRLCFSLNPALLLPLKTQVIPPESFRR 60
           MRDYC  FN S+ K LKS FLFFF +L  VF +  LC SLNPAL+LPLKTQVIPPES RR
Sbjct: 1   MRDYCFAFNFSSVKFLKSCFLFFFCTLFSVFHSIHLCSSLNPALVLPLKTQVIPPESVRR 60

Query: 61  SPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQNSSASSSTFHPVRST 120
           SPDKLPFRHNISLTVSLTVG+PPQNVTMVIDTGSELSWL CN SQNSS+SSSTF+PV S+
Sbjct: 61  SPDKLPFRHNISLTVSLTVGTPPQNVTMVIDTGSELSWLHCNTSQNSSSSSSTFNPVWSS 120

Query: 121 SYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYFGNSGIP 180
           SY+PIPCSS TCT+QTRDFPI  SCDS+Q CHATLSYADASSSEGNLA DTFY G+SGIP
Sbjct: 121 SYSPIPCSSSTCTDQTRDFPIRPSCDSNQFCHATLSYADASSSEGNLATDTFYIGSSGIP 180

Query: 181 NVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240
           NV+FGCMDSIFSSN+EEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD
Sbjct: 181 NVVFGCMDSIFSSNSEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240

Query: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPDHTGAGQT 300
           ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKV+HKL+PIPESVFEPDHTGAGQT
Sbjct: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVAHKLLPIPESVFEPDHTGAGQT 300

Query: 301 MVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLPP 360
           MVDSGTQFTFLLGPAYTALRD FLNKTAGSLRV+ED NFVFQGAMDLCYRVP  QTRLPP
Sbjct: 301 MVDSGTQFTFLLGPAYTALRDHFLNKTAGSLRVYEDSNFVFQGAMDLCYRVPTNQTRLPP 360

Query: 361 LPSVTLVFSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQNV 420
           LPSVTLVF GAEMTVTGDRILYRVPGE RG+DSIHCFTFGNSDLLGVEAFVIGHLHQQNV
Sbjct: 361 LPSVTLVFRGAEMTVTGDRILYRVPGERRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNV 420

Query: 421 WMEFDLKKSRIGLAKIRCDLAGQKLGMGL 450
           WMEFDLKKSRIGLA+IRCDLAGQKLGMGL
Sbjct: 421 WMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Sed0004437 vs. ExPASy TrEMBL
Match: A0A6J1G928 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111452070 PE=3 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 4.3e-232
Identity = 396/449 (88.20%), Postives = 421/449 (93.76%), Query Frame = 0


Query: 1   MRDYCINFNPSNYKSLKSSFLFFFTSLILVFQNPRLCFSLNPALLLPLKTQVIPPESFRR 60
           MRDYCI+ NP N   LK  F FF  SL+ VFQNP LCFSLNPAL+LPLKTQVIPPES  R
Sbjct: 1   MRDYCISLNPLNNPFLKYCFSFFLISLVFVFQNPLLCFSLNPALILPLKTQVIPPESVPR 60

Query: 61  SPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQNSSASSSTFHPVRST 120
           SPDKLPFRHN+SLTVSLTVG+PPQNVTMVIDTGSELSWL CNRSQNSS+SSSTF+PV+S+
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVQSS 120

Query: 121 SYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYFGNSGIP 180
           SY PIPCSS TCT+QTRDFPIPASCDS+QLCHATLSYADASSSEGNLAADTFY GNSGIP
Sbjct: 121 SYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGIP 180

Query: 181 NVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240
           NV+FGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDF GLLLLGD
Sbjct: 181 NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFPGLLLLGD 240

Query: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPDHTGAGQT 300
           ANFSWLAPLNYTP+I++STPLPYFDRVAYTVQL+GIKVS+KL+PIPES FEPDHTGAGQT
Sbjct: 241 ANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESAFEPDHTGAGQT 300

Query: 301 MVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLPP 360
           MVDSGTQFTFLLG AYT LRDEFLN+TAGSLRV EDPNFVFQGAMDLCYRVP  QTRLPP
Sbjct: 301 MVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVSEDPNFVFQGAMDLCYRVPTNQTRLPP 360

Query: 361 LPSVTLVFSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQNV 420
           LP+VTLVF GAEMTVTGDRILYRV GE RG+D+I+CFTFGNSDLLGVEA+VIGHLHQQNV
Sbjct: 361 LPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAYVIGHLHQQNV 420

Query: 421 WMEFDLKKSRIGLAKIRCDLAGQKLGMGL 450
           WMEFDLKKSRIGLA+IRCDLAGQKLGMGL
Sbjct: 421 WMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Sed0004437 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 587.0 bits (1512), Expect = 1.3e-167
Identity = 289/415 (69.64%), Postives = 346/415 (83.37%), Query Frame = 0


Query: 39  SLNPALLLPLKTQVIPPESFRRSPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSW 98
           S +  L+LPLKT++ P +   R  DKL F HN++LTV+LTVG+PPQN++MVIDTGSELSW
Sbjct: 41  SSSQTLVLPLKTRITPTD--HRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELSW 100

Query: 99  LRCNRSQNSSASSSTFHPVRSTSYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYA 158
           LRCNRS N +  ++ F P RS+SY+PIPCSSPTC  +TRDF IPASCDSD+LCHATLSYA
Sbjct: 101 LRCNRSSNPNPVNN-FDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSYA 160

Query: 159 DASSSEGNLAADTFYFGNS-GIPNVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQ 218
           DASSSEGNLAA+ F+FGNS    N+IFGCM S+  S+ EED+K TGL+GMNRGSLSF+SQ
Sbjct: 161 DASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQ 220

Query: 219 MGFPKFSYCIS-EYDFSGLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGI 278
           MGFPKFSYCIS   DF G LLLGD+NF+WL PLNYTPLI +STPLPYFDRVAYTVQL GI
Sbjct: 221 MGFPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGI 280

Query: 279 KVSHKLIPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFED 338
           KV+ KL+PIP+SV  PDHTGAGQTMVDSGTQFTFLLGP YTALR  FLN+T G L V+ED
Sbjct: 281 KVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYED 340

Query: 339 PNFVFQGAMDLCYRVPMTQTR---LPPLPSVTLVFSGAEMTVTGDRILYRVPGETRGSDS 398
           P+FVFQG MDLCYR+   + R   L  LP+V+LVF GAE+ V+G  +LYRVP  T G+DS
Sbjct: 341 PDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGNDS 400

Query: 399 IHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLKKSRIGLAKIRCDLAGQKLGMG 449
           ++CFTFGNSDL+G+EA+VIGH HQQN+W+EFDL++SRIGLA + CD++GQ+LG+G
Sbjct: 401 VYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

[Close]

BLAST of Sed0004437 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 540.0 bits (1390), Expect = 1.8e-153
Identity = 273/443 (61.63%), Postives = 336/443 (75.85%), Query Frame = 0


Query: 11  SNYKSLKSSFLFFFTSLILVFQNPRLC--FSLNPALLLPLKTQVIPPESFRRSPDKLPFR 70
           S+  SL  S  F   S++L+      C   S N  LL  LKTQ +P    + S DKL FR
Sbjct: 5   SSSSSLSLSKNFLRISVLLLIFPLTFCKTSSTNQTLLFSLKTQKLP----QSSSDKLSFR 64

Query: 71  HNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQNSSASSSTFHPVRSTSYAPIPCS 130
           HN++LTV+L VG PPQN++MV+DTGSELSWL C +S N     S F+PV S++Y+P+PCS
Sbjct: 65  HNVTLTVTLAVGDPPQNISMVLDTGSELSWLHCKKSPN---LGSVFNPVSSSTYSPVPCS 124

Query: 131 SPTCTNQTRDFPIPASCD-SDQLCHATLSYADASSSEGNLAADTFYFGNSGIPNVIFGCM 190
           SP C  +TRD PIPASCD    LCH  +SYADA+S EGNLA +TF  G+   P  +FGCM
Sbjct: 125 SPICRTRTRDLPIPASCDPKTHLCHVAISYADATSIEGNLAHETFVIGSVTRPGTLFGCM 184

Query: 191 DSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLA 250
           DS  SSN+EED+K+TGLMGMNRGSLSFV+Q+GF KFSYCIS  D SG LLLGDA++SWL 
Sbjct: 185 DSGLSSNSEEDAKSTGLMGMNRGSLSFVNQLGFSKFSYCISGSDSSGFLLLGDASYSWLG 244

Query: 251 PLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIPIPESVFEPDHTGAGQTMVDSGTQ 310
           P+ YTPL+  STPLPYFDRVAYTVQLEGI+V  K++ +P+SVF PDHTGAGQTMVDSGTQ
Sbjct: 245 PIQYTPLVLQSTPLPYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQ 304

Query: 311 FTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRV-PMTQTRLPPLPSVTL 370
           FTFL+GP YTAL++EF+ +T   LR+ +DP+FVFQG MDLCY+V   T+     LP V+L
Sbjct: 305 FTFLMGPVYTALKNEFITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNFSGLPMVSL 364

Query: 371 VFSGAEMTVTGDRILYRVPGE-TRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFD 430
           +F GAEM+V+G ++LYRV G  + G + ++CFTFGNSDLLG+EAFVIGH HQQNVWMEFD
Sbjct: 365 MFRGAEMSVSGQKLLYRVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFD 424

Query: 431 LKKSRIGLA-KIRCDLAGQKLGM 448
           L KSR+G A  +RCDLA Q+LG+
Sbjct: 425 LAKSRVGFAGNVRCDLASQRLGL 440

[Close]

BLAST of Sed0004437 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 258.5 bits (659), Expect = 1.0e-68
Identity = 152/398 (38.19%), Postives = 223/398 (56.03%), Query Frame = 0


Query: 54  PPESFRRSPDKLPFRHNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQNSSASSST 113
           PP +FR       F+++++L +SL +G+PPQ   MV+DTGS+LSW++C+R +      ++
Sbjct: 58  PPYNFRSR-----FKYSMALIISLPIGTPPQAQQMVLDTGSQLSWIQCHRKKLPPKPKTS 117

Query: 114 FHPVRSTSYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFY 173
           F P  S+S++ +PCS P C  +  DF +P SCDS++LCH +  YAD + +EGNL  +   
Sbjct: 118 FDPSLSSSFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKIT 177

Query: 174 FGNSGI-PNVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISE--- 233
           F N+ I P +I GC          E S + G++GMNRG LSFVSQ    KFSYCI     
Sbjct: 178 FSNTEITPPLILGCA--------TESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSN 237

Query: 234 ---YDFSGLLLLGD----ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKL 293
              +  +G   LGD      F +++ L +      S  +P  D +AYTV + GI+   K 
Sbjct: 238 RPGFTPTGSFYLGDNPNSHGFKYVSLLTFPE----SQRMPNLDPLAYTVPMIGIRFGLKK 297

Query: 294 IPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQ 353
           + I  SVF PD  G+GQTMVDSG++FT L+  AY  +R E + +    L+      +V+ 
Sbjct: 298 LNISGSVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRVGRRLK----KGYVYG 357

Query: 354 GAMDLCY--RVPMTQTRLPPLPSVTLVFSGAEMTVTGDRILYRVPGETRGSDSIHCFTFG 413
           G  D+C+   V M    +  L  V +   G E+ V  +R+L  V G       IHC   G
Sbjct: 358 GTADMCFDGNVAMIPRLIGDL--VFVFTRGVEILVPKERVLVNVGG------GIHCVGIG 417

Query: 414 NSDLLGVEAFVIGHLHQQNVWMEFDLKKSRIGLAKIRC 439
            S +LG  + +IG++HQQN+W+EFD+   R+G AK  C
Sbjct: 418 RSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFAKADC 426

[Close]

BLAST of Sed0004437 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 239.2 bits (609), Expect = 6.4e-63
Identity = 154/458 (33.62%), Postives = 240/458 (52.40%), Query Frame = 0


Query: 16  LKSSFLFFFTSLILVFQNPRLCFSLNPALLLPLKTQVIPP----ESFRR--------SPD 75
           LK  ++FFF        +  L +S + +L  PL +  + P     SF+         SP 
Sbjct: 10  LKLLYIFFF-----FCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPP 69

Query: 76  KLPF------RHNISLTVSLTVGSPPQNVTMVIDTGSELSWLRCNRSQNSS---ASSSTF 135
             P+      +++++L +SL +G+P Q+  +V+DTGS+LSW++C+  +        +++F
Sbjct: 70  SSPYTFRSNIKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPTTSF 129

Query: 136 HPVRSTSYAPIPCSSPTCTNQTRDFPIPASCDSDQLCHATLSYADASSSEGNLAADTFYF 195
            P  S+S++ +PCS P C  +  DF +P SCDS++LCH +  YAD + +EGNL  + F F
Sbjct: 130 DPSLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTF 189

Query: 196 GNS-GIPNVIFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYD-- 255
            NS   P +I GC         +E +   G++GMN G LSF+SQ    KFSYCI      
Sbjct: 190 SNSQTTPPLILGCA--------KESTDEKGILGMNLGRLSFISQAKISKFSYCIPTRSNR 249

Query: 256 ----FSGLLLLGD----ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLI 315
                +G   LGD      F +++ L +      S  +P  D +AYTV L+GI++  K +
Sbjct: 250 PGLASTGSFYLGDNPNSRGFKYVSLLTF----PQSQRMPNLDPLAYTVPLQGIRIGQKRL 309

Query: 316 PIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQG 375
            IP SVF PD  G+GQTMVDSG++FT L+  AY  +++E +      L+      +V+  
Sbjct: 310 NIPGSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRLK----KGYVYGS 369

Query: 376 AMDLCYRVPMTQTRLPPLPSVTLVFS-GAEMTVTGDRILYRVPGETRGSDSIHCFTFGNS 435
             D+C+    +      +  +   F  G E+ V    +L  V G       IHC   G S
Sbjct: 370 TADMCFDGNHSMEIGRLIGDLVFEFGRGVEILVEKQSLLVNVGG------GIHCVGIGRS 429

Query: 436 DLLGVEAFVIGHLHQQNVWMEFDLKKSRIGLAKIRCDL 441
            +LG  + +IG++HQQN+W+EFD+   R+G +K  C L
Sbjct: 430 SMLGAASNIIGNVHQQNLWVEFDVTNRRVGFSKAECRL 440

[Close]

BLAST of Sed0004437 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 147.1 bits (370), Expect = 3.3e-35
Identity = 121/371 (32.61%), Postives = 182/371 (49.06%), Query Frame = 0


Query: 77  LTVGSPPQNVTMVIDTGSELSWLRCNRSQNS-SASSSTFHPVRSTSYAPIPCSSPTCTNQ 136
           L VG+P + V MV+DTGS++ WL+C   +   S S   F P +S +YA IPCSSP C   
Sbjct: 146 LGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRL 205

Query: 137 TRDFPIPASCDS-DQLCHATLSYADASSSEGNLAADTFYFGNSGIPNVIFGCMDSIFSSN 196
                  A C++  + C   +SY D S + G+ + +T  F  + +  V  GC       N
Sbjct: 206 D-----SAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGC----GHDN 265

Query: 197 NEEDSKNTGLMGMNRGSLSFVSQMGF---PKFSYCISEYDFS---GLLLLGDANFSWLAP 256
                   GL+G+ +G LSF  Q G     KFSYC+ +   S     ++ G+A  S +A 
Sbjct: 266 EGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIA- 325

Query: 257 LNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLIP-IPESVFEPDHTGAGQTMVDSGTQ 316
             +TPL+      P  D   Y V L GI V    +P +  S+F+ D  G G  ++DSGT 
Sbjct: 326 -RFTPLLSN----PKLD-TFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTS 385

Query: 317 FTFLLGPAYTALRDEFLNKTAGSLRVFEDPNFVFQGAMDLCYRVPMTQTRLPPLPSVTLV 376
            T L+ PAY A+RD F     G+  +   P+F      D C+   ++      +P+V L 
Sbjct: 386 VTRLIRPAYIAMRDAF---RVGAKTLKRAPDF---SLFDTCF--DLSNMNEVKVPTVVLH 445

Query: 377 FSGAEMTVTGDRILYRVPGETRGSDSIHCFTFGNSDLLGVEAFVIGHLHQQNVWMEFDLK 436
           F GA++++      Y +P +T G     CF F  + + G+   +IG++ QQ   + +DL 
Sbjct: 446 FRGADVSLPATN--YLIPVDTNGK---FCFAFAGT-MGGLS--IIGNIQQQGFRVVYDLA 484

Query: 437 KSRIGLAKIRC 439
            SR+G A   C
Sbjct: 506 SSRVGFAPGGC 484

The following BLAST results are available for this feature:

BLAST of Sed0004437 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905417.1	7.7e-236	89.98	aspartic proteinase PCS1 [Benincasa hispida]	[more]
XP_022983486.1	1.3e-235	90.65	aspartic proteinase PCS1 [Cucurbita maxima]	[more]
XP_023528618.1	2.8e-233	90.00	aspartic proteinase PCS1 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023528617.1	2.8e-233	90.00	aspartic proteinase PCS1 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022935018.1	3.6e-233	90.00	aspartic proteinase PCS1 [Cucurbita moschata] >KAG6580532.1 Aspartic proteinase ...	[more]

BLAST of Sed0004437 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZL3	1.8e-166	69.64	Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1	[more]
Q766C3	1.6e-37	31.35	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	4.2e-35	27.79	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LNJ3	4.7e-34	32.61	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q6F4N5	2.0e-32	31.43	Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1	[more]

BLAST of Sed0004437 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J2F5	6.4e-236	90.65	aspartic proteinase PCS1 OS=Cucurbita maxima OX=3661 GN=LOC111482077 PE=3 SV=1	[more]
A0A6J1F9C1	1.7e-233	90.00	aspartic proteinase PCS1 OS=Cucurbita moschata OX=3662 GN=LOC111442007 PE=3 SV=1	[more]
A0A6J1F3C7	1.7e-233	90.00	Laccase OS=Cucurbita moschata OX=3662 GN=LOC111441767 PE=3 SV=1	[more]
A0A0A0LAI7	2.5e-232	89.31	Aspartic proteinase nepenthesin-2 OS=Cucumis sativus OX=3659 GN=Csa_3G734110 PE=...	[more]
A0A6J1G928	4.3e-232	88.20	aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111452070 PE=3...	[more]

BLAST of Sed0004437 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G02190.1	1.3e-167	69.64	Eukaryotic aspartyl protease family protein	[more]
AT2G39710.1	1.8e-153	61.63	Eukaryotic aspartyl protease family protein	[more]
AT1G66180.1	1.0e-68	38.19	Eukaryotic aspartyl protease family protein	[more]
AT5G37540.1	6.4e-63	33.62	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	3.3e-35	32.61	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 410..425 score: 18.31 coord: 300..311 score: 32.91 coord: 79..99 score: 43.59
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR47965	ASPARTYL PROTEASE-RELATED	coord: 20..447
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 67..240 e-value: 1.1E-35 score: 125.3
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 244..441 e-value: 6.6E-43 score: 148.4
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 74..438
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 75..240 e-value: 1.6E-39 score: 135.9
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 268..434 e-value: 4.3E-36 score: 124.1
None	No IPR available	PANTHER	PTHR47965:SF64	ASPARTIC PROTEINASE PCS1	coord: 20..447
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 88..99
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 73..434 score: 34.564198
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 74..438 e-value: 1.54458E-69 score: 219.827

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0004437.1	Sed0004437.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046274	lignin catabolic process
biological_process	GO:0012501	programmed cell death
biological_process	GO:0006508	proteolysis
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0052716	hydroquinone:oxygen oxidoreductase activity