Sed0004377 (gene) Chayote v1

BLAST of Sed0004377 vs. NCBI nr
Match: XP_023547692.1 (polygalacturonase QRT2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763.8 bits (1971), Expect = 8.0e-217
Identity = 371/454 (81.72%), Postives = 408/454 (89.87%), Query Frame = 0

Query: 1   MCRGFLGRSRLLLFLL-VVVFELLLTCSSSYDNVDPPRAISNG-----DEEHDEYSSEFS 60
           MCRG LGR   L FL+ +VVFE    CSSSY +VDPP  I+ G     D +H  YSS+FS
Sbjct: 1   MCRGLLGRRCRLPFLIAIVVFEFFSACSSSYADVDPPPLINRGDGKDRDHQHYGYSSKFS 60

Query: 61  SMLKTRLEKTSWSPMSSPEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPTER 120
           SML TRLEK + SP++S EIFNVDDYGAMGDGEDDS+AFKEAWKEACSSTNAIFLVP +R
Sbjct: 61  SMLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSQAFKEAWKEACSSTNAIFLVPNDR 120

Query: 121 TYHLKPITFPGPCNSPLVFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEGKGTING 180
           TYHLKPITF GPCNSP VF IEGTIKASPHISDYEKDRRHWI+F+++++FT+EGKGTING
Sbjct: 121 TYHLKPITFSGPCNSPFVFKIEGTIKASPHISDYEKDRRHWIVFENLAEFTVEGKGTING 180

Query: 181 NGRIWWQNSCKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNVKALN 240
           NGR WW NSCKV+KTLPC EAPTAVTFYQCTNLRVEGL FRN+QKMHLSFQKC+NVKALN
Sbjct: 181 NGRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCSNVKALN 240

Query: 241 LWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGIS 300
           LWIYAPGNSPNTDGIHVTATQ I+I+NCLIMTGDDCISIVSGSKNVRAKGITCGPGHGIS
Sbjct: 241 LWIYAPGNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGIS 300

Query: 301 IGSLGAGKSEAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVTNPII 360
           IGSLGAGKSEAEVSNVV+DTAKF GTSNGVRIKTWQGGKGYA+NIIF+NIVM+NVTNPII
Sbjct: 301 IGSLGAGKSEAEVSNVVVDTAKFKGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVTNPII 360

Query: 361 IDQNYCDQKDPCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQDINLV 420
           IDQNYCDQK+PCTQQA+AVQVSNV+YQNIRGTS S+VAV FDCSKS PC+ I+LQDI+LV
Sbjct: 361 IDQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCQEILLQDIDLV 420

Query: 421 HRGK-DEKPHEAEASCKNVKLKNRGMVSPQCNGR 448
           HR K D+KP +AEASCKN +LKNRG VSPQC GR
Sbjct: 421 HRDKADDKPTQAEASCKNARLKNRGRVSPQCPGR 454

BLAST of Sed0004377 vs. NCBI nr
Match: XP_038899303.1 (polygalacturonase QRT2-like [Benincasa hispida])

HSP 1 Score: 761.1 bits (1964), Expect = 5.2e-216
Identity = 374/455 (82.20%), Postives = 409/455 (89.89%), Query Frame = 0

Query: 2   CRGFLGRSRLLL---FLLVVVFELLLTCSSSYDNVDPPRAISNGD-EEHD----EYSSEF 61
           C+ F  R  LLL    + + + E   TCS SY ++DPP +IS+GD E+HD    EYSSEF
Sbjct: 4   CQLFSHRCCLLLLSFLITMAISEFFSTCSGSYADIDPPPSISSGDKEDHDQHYYEYSSEF 63

Query: 62  SSMLKTRLEKTSWSPMSSPEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPTE 121
           SSML+TRLEK + SP++S EIFNVDDYGAMGDGEDDSEAFKEAWK+ACSSTNAIFLVP +
Sbjct: 64  SSMLRTRLEKMAPSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKDACSSTNAIFLVPCD 123

Query: 122 RTYHLKPITFPGPCNSPLVFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEGKGTIN 181
           R YHLKPITF GPCNSPL+F IEGTIKASPHISDYEKDRRHWIIFQ+IS+F +EGKG IN
Sbjct: 124 RIYHLKPITFSGPCNSPLLFKIEGTIKASPHISDYEKDRRHWIIFQNISNFRVEGKGIIN 183

Query: 182 GNGRIWWQNSCKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNVKAL 241
           GNGR WW NSCKV+KTLPC EAPTAVTFY+CTNLRVEG+ FRN+Q+MHLSFQKCNNVKAL
Sbjct: 184 GNGRKWWSNSCKVNKTLPCKEAPTAVTFYECTNLRVEGIRFRNAQRMHLSFQKCNNVKAL 243

Query: 242 NLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGI 301
           NLWIYAPGNSPNTDGIHVT TQ IVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGI
Sbjct: 244 NLWIYAPGNSPNTDGIHVTGTQSIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGI 303

Query: 302 SIGSLGAGKSEAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVTNPI 361
           SIGSLGAGKSEAEVSNVV+DTAKFSGTSNGVRIKTWQGGKGYAQNIIF+NIVM+NVTNPI
Sbjct: 304 SIGSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMDNVTNPI 363

Query: 362 IIDQNYCDQKDPCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQDINL 421
           IIDQNYCDQK+PCTQQADAVQVSNV+YQNIRGTSAS+VAV FDCSKS PC+GI+LQDINL
Sbjct: 364 IIDQNYCDQKEPCTQQADAVQVSNVMYQNIRGTSASEVAVKFDCSKSFPCQGILLQDINL 423

Query: 422 VHRGK-DEKPHEAEASCKNVKLKNRGMVSPQCNGR 448
           V +GK D+K  +AEASCKNVKLKNRG VSPQC GR
Sbjct: 424 VRKGKNDDKSTQAEASCKNVKLKNRGRVSPQCAGR 458

BLAST of Sed0004377 vs. NCBI nr
Match: XP_004135935.1 (polygalacturonase QRT2 isoform X2 [Cucumis sativus] >KAE8646400.1 hypothetical protein Csa_016612 [Cucumis sativus])

HSP 1 Score: 760.8 bits (1963), Expect = 6.8e-216
Identity = 371/458 (81.00%), Postives = 413/458 (90.17%), Query Frame = 0

Query: 1   MCRGFLGRSRLL-----LFLLVVVFELLLTCSSSYDNVDPP-RAISNGDEE----HDEYS 60
           MCR  LGR   L     LF++VV+F++L TCS SY +VD P  +IS+GD+E    H +YS
Sbjct: 1   MCRPLLGRPCCLSLLTFLFVVVVIFDILSTCSGSYPDVDNPLPSISSGDKEVDGQHYKYS 60

Query: 61  SEFSSMLKTRLEKTSWSPMSSPEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLV 120
           SEFSSML+TRLEK + SP++SPEIFNVDDYGAMGDGEDDSEAFKE WK+ACSSTNAIFLV
Sbjct: 61  SEFSSMLRTRLEKMASSPLASPEIFNVDDYGAMGDGEDDSEAFKETWKDACSSTNAIFLV 120

Query: 121 PTERTYHLKPITFPGPCNSPLVFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEGKG 180
           P +R YHLKPITF GPCNSPL+F IEGTIKASPHISDYEKDRRHWIIFQ++    +EGKG
Sbjct: 121 PCDRVYHLKPITFTGPCNSPLLFKIEGTIKASPHISDYEKDRRHWIIFQNLLGLRVEGKG 180

Query: 181 TINGNGRIWWQNSCKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNV 240
            INGNGR WW NSCKV+KTLPC EAPTAVTF+QCTNLRVEGL FRN+Q+MHLSFQKCNNV
Sbjct: 181 IINGNGRKWWLNSCKVNKTLPCKEAPTAVTFFQCTNLRVEGLRFRNAQQMHLSFQKCNNV 240

Query: 241 KALNLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPG 300
           KALNLWI++PGNSPNTDGIHVT TQFIVIKNCLIMTGDDC+SIVSGSKNVRAKGITCGPG
Sbjct: 241 KALNLWIHSPGNSPNTDGIHVTGTQFIVIKNCLIMTGDDCLSIVSGSKNVRAKGITCGPG 300

Query: 301 HGISIGSLGAGKSEAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVT 360
           HGISIGSLGAGK+EAEVSNVV+DTAKFSGT+NGVRIKTWQGGKGYAQNIIF+NIVM+NVT
Sbjct: 301 HGISIGSLGAGKTEAEVSNVVVDTAKFSGTTNGVRIKTWQGGKGYAQNIIFQNIVMDNVT 360

Query: 361 NPIIIDQNYCDQKDPCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQD 420
           NPIIIDQNYCDQK+PCTQQADAV+VSNVVYQNIRGTSAS+VAV F+CSKS+PC+GI+LQD
Sbjct: 361 NPIIIDQNYCDQKEPCTQQADAVEVSNVVYQNIRGTSASEVAVKFNCSKSIPCQGILLQD 420

Query: 421 INLVHRGK-DEKPHEAEASCKNVKLKNRGMVSPQCNGR 448
           INLVH+GK D+K  +AEASCKNVKLKNRG VSP C GR
Sbjct: 421 INLVHKGKNDDKSAQAEASCKNVKLKNRGRVSPLCGGR 458

BLAST of Sed0004377 vs. NCBI nr
Match: XP_022953263.1 (polygalacturonase QRT2-like [Cucurbita moschata])

HSP 1 Score: 759.2 bits (1959), Expect = 2.0e-215
Identity = 372/458 (81.22%), Postives = 406/458 (88.65%), Query Frame = 0

Query: 1   MCRGFLGRSRLLLFLL-----VVVFELLLTCSSSYDNVDPPRAISNG-----DEEHDEYS 60
           MCRG LGR   LL LL     +VVF     CSSSY +VDPP  I  G     D +H  YS
Sbjct: 1   MCRGLLGRRCRLLLLLPFLVAIVVFGFFSVCSSSYADVDPPPLIIRGDGKDRDHQHYGYS 60

Query: 61  SEFSSMLKTRLEKTSWSPMSSPEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLV 120
           S+FSSML TRLEK + SP++S EIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLV
Sbjct: 61  SKFSSMLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLV 120

Query: 121 PTERTYHLKPITFPGPCNSPLVFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEGKG 180
           P +RTYHLKPITF GPCNSP  F IEGTIKASPHISDYEKDRRHWIIF+++S+FT+EGKG
Sbjct: 121 PIDRTYHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEGKG 180

Query: 181 TINGNGRIWWQNSCKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNV 240
           TINGNGR WW NSCKV+KTLPC EAPTAVTFYQCTNLRVEGL FRN+QKMHLSFQKC+NV
Sbjct: 181 TINGNGRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCSNV 240

Query: 241 KALNLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPG 300
           KALNLWIYAPGNSPNTDGIHVTATQ I+I+NCLIMTGDDCISIVSGSKNVRAKGITCGPG
Sbjct: 241 KALNLWIYAPGNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPG 300

Query: 301 HGISIGSLGAGKSEAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVT 360
           HGISIGSLGAGKSEAEVSNVV+DTAKF+GTSNGVRIKTWQGGKGYA+NIIF+NIVM+NVT
Sbjct: 301 HGISIGSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVT 360

Query: 361 NPIIIDQNYCDQKDPCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQD 420
           NPIIIDQNYCDQK+PCTQQA+AVQVSNV+YQNIRGTS S+VAV FDCS+S PC+ I+LQD
Sbjct: 361 NPIIIDQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSESFPCQEILLQD 420

Query: 421 INLVHRGK-DEKPHEAEASCKNVKLKNRGMVSPQCNGR 448
           I+LVHR K D+KP +AEASCKN +LKNRG VSPQC GR
Sbjct: 421 IDLVHRDKADDKPTQAEASCKNARLKNRGRVSPQCPGR 458

BLAST of Sed0004377 vs. NCBI nr
Match: XP_022991888.1 (polygalacturonase QRT2-like [Cucurbita maxima])

HSP 1 Score: 752.7 bits (1942), Expect = 1.8e-213
Identity = 371/460 (80.65%), Postives = 407/460 (88.48%), Query Frame = 0

Query: 1   MCRGFLG---RSRLLLFL----LVVVFELLLTCSSSYDNVDPPRAISNG-----DEEHDE 60
           MCRG LG   R RLLL L     +VVFE    CSSSY +VDPP  I+ G     D+++  
Sbjct: 1   MCRGLLGCRCRCRLLLLLPFLIAIVVFEFFSLCSSSYADVDPPLLINRGDGKDRDQQYHG 60

Query: 61  YSSEFSSMLKTRLEKTSWSPMSSPEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIF 120
           YSS+FSSML TRLEK + SP++S EIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIF
Sbjct: 61  YSSKFSSMLTTRLEKMAPSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIF 120

Query: 121 LVPTERTYHLKPITFPGPCNSPLVFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEG 180
           LVP +RTYHLKPITF GPCNSP  F IEGTIKASPHISDYEKDRRHWIIF+++++FT+EG
Sbjct: 121 LVPNDRTYHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLTEFTVEG 180

Query: 181 KGTINGNGRIWWQNSCKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCN 240
           KG INGNGR WW NSCKV+KTLPC EAPTAVTFYQCTNLRVEGL FRN+QKMHLSFQKC+
Sbjct: 181 KGIINGNGRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCS 240

Query: 241 NVKALNLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCG 300
           NVKALNLWIYAPGNSPNTDGIHVTATQ I+I+NCLIMTGDDCISIVSGSKNVRAKGITCG
Sbjct: 241 NVKALNLWIYAPGNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCG 300

Query: 301 PGHGISIGSLGAGKSEAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNN 360
           PGHGISIGSLGAGKSEAEVS+VV+DTAKF+GTSNGVRIKTWQGGKGYAQNIIF+NIVM+N
Sbjct: 301 PGHGISIGSLGAGKSEAEVSDVVVDTAKFTGTSNGVRIKTWQGGKGYAQNIIFQNIVMDN 360

Query: 361 VTNPIIIDQNYCDQKDPCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIIL 420
           VTNPIIIDQNYCDQK+PCTQQA+AVQVSNV+YQNIRGTS S+VAV FDCSKS PC  I+L
Sbjct: 361 VTNPIIIDQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCLEILL 420

Query: 421 QDINLVHRGK-DEKPHEAEASCKNVKLKNRGMVSPQCNGR 448
           QDI+LVHR K D+K  +AEASCKN +LKNRG VSPQC GR
Sbjct: 421 QDIDLVHRDKTDDKSTQAEASCKNARLKNRGRVSPQCPGR 460

BLAST of Sed0004377 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 8.3e-132
Identity = 222/376 (59.04%), Postives = 282/376 (75.00%), Query Frame = 0

Query: 69  MSSPEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPTERTYHLKPITFPGPCN 128
           + + +  NVDD+GA GDG DD++AF++AWK ACSST++  L+  ++ Y ++PI+F GPC 
Sbjct: 84  LDASKTVNVDDFGAKGDGRDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCK 143

Query: 129 SPLVFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEGKGTINGNGRIWWQNSCKVDK 188
           S L   I GTI+AS   SDY KD RHW++F  + +  +EG GTINGNG+IWWQNSCK +K
Sbjct: 144 SGLTMQIYGTIEASDDRSDYRKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNK 203

Query: 189 TLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNVKALNLWIYAPGNSPNTDG 248
            LPC +APTA+TFY+  ++ V+ L   N+Q++H+SF  C NV+A NL + AP NSPNTDG
Sbjct: 204 ALPCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTDG 263

Query: 249 IHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVS 308
           IHVT TQ I I +C+I TGDDCISIV+GS+ VR   ITCGPGHGISIGSLG G SEA VS
Sbjct: 264 IHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHVS 323

Query: 309 NVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVTNPIIIDQNYCDQKDPCTQ 368
           +VV++ AK  GT+NGVRIKTWQGG G A NI F+N+ M+NV NPIIIDQNYCDQ  PC +
Sbjct: 324 DVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQE 383

Query: 369 QADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQDINLVHRGKDEKPHEAEAS 428
           Q+ AVQV NVVYQNI+GT AS VA+ FDCSK  PC+GI+L+D++L    + E    A+A 
Sbjct: 384 QSSAVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDL----EIEGGAAAKAL 443

Query: 429 CKNVKLKNRGMVSPQC 445
           C NV+L   G+VSP C
Sbjct: 444 CNNVELSETGVVSPHC 455

BLAST of Sed0004377 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 465.3 bits (1196), Expect = 7.7e-130
Identity = 235/441 (53.29%), Postives = 311/441 (70.52%), Query Frame = 0

Query: 12  LLFLLVVVFELLLTCSSSY------DNVDPPRAISNGDEEHDEYSSEFSSMLKTRLEKTS 71
           ++ L V +   L +C SSY      D           + +H    +  +S LK R     
Sbjct: 5   IIILSVFLLTFLPSCFSSYPFNHRDDLFMSSNVYYETNRQHQHGHNTRNSHLKNR---HG 64

Query: 72  WSPMSSPEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLV-PTERTYHLKPITFP 131
           ++P SSP  FNV+ +GA  +G DDS+AF +AW+ ACSST  +++V P  R Y LK +TF 
Sbjct: 65  YAPRSSPRSFNVNTFGAKANGNDDSKAFMKAWEAACSSTGIVYIVAPKNRDYMLKAVTFS 124

Query: 132 GPCNSPL-VFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEGKGTINGNGRIWWQNS 191
           GPC S L +F I G I+A  + SDY K+RRHWI+F+++++  +EG G I+GNG IWW  S
Sbjct: 125 GPCKSSLIIFKIYGRIEAWENPSDY-KERRHWIVFENVNNLRVEGGGRIDGNGHIWWPKS 184

Query: 192 CKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNVKALNLWIYAPGNS 251
           CK++  LPC  APTAVTF +C NLRV  +   N+Q+MHL+FQ C NVKALNL + +P +S
Sbjct: 185 CKINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADS 244

Query: 252 PNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKS 311
           PNTDGIHV+ TQ I+I++ ++ TGDDCISIVSGS+NVRA GITCGPGHGISIGSLG   S
Sbjct: 245 PNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNS 304

Query: 312 EAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVTNPIIIDQNYCDQK 371
           EA VSNVV++ A   GT+NGVRIKTWQGG G A+NIIF++I+M NVTNPIII+Q+YCD+ 
Sbjct: 305 EAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRV 364

Query: 372 DPCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQDINLVHRGKDEKPH 431
           + C +Q  AVQVSNV+Y+NI+GTS+  +AV F CSK++PC GI +Q++ LV + + +   
Sbjct: 365 EACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQD--- 424

Query: 432 EAEASCKNVKLKNRGMVSPQC 445
            ++ASC NVKL  RG VSP C
Sbjct: 425 VSKASCSNVKLDTRGNVSPLC 438

BLAST of Sed0004377 vs. ExPASy Swiss-Prot
Match: P05117 (Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 7.7e-122
Identity = 226/440 (51.36%), Postives = 300/440 (68.18%), Query Frame = 0

Query: 12  LLFLLVVVFELLLTCSSSY--DNVDPPRAISNGDEEHDEYSSEFSSMLKTRLEKTS---W 71
           +L L+++    + TC S+   DN+      +  ++E       + S L   +E  +    
Sbjct: 8   ILLLIIIFASSISTCRSNVIDDNLFKQVYDNILEQEFAHDFQAYLSYLSKNIESNNNIDK 67

Query: 72  SPMSSPEIFNVDDYGAMGDGED-DSEAFKEAWKEACSSTNAI-FLVPTERTYHLKPITFP 131
              +  ++ NV  +GA GDG+  D+ AF++AW EACSS   + F+VP  + Y LK ITF 
Sbjct: 68  VDKNGIKVINVLSFGAKGDGKTYDNIAFEQAWNEACSSRTPVQFVVPKNKNYLLKQITFS 127

Query: 132 GPCNSPLVFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEGKGTINGNGRIWWQNSC 191
           GPC S +   I G+++AS  ISDY KDRR WI F  + +  + G GTINGNG++WW +SC
Sbjct: 128 GPCRSSISVKIFGSLEASSKISDY-KDRRLWIAFDSVQNLVVGGGGTINGNGQVWWPSSC 187

Query: 192 KVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNVKALNLWIYAPGNSP 251
           K++K+LPC +APTA+TF+ C NL+V  L  +N+Q++H+ F+ C NV A NL I A   SP
Sbjct: 188 KINKSLPCRDAPTALTFWNCKNLKVNNLKSKNAQQIHIKFESCTNVVASNLMINASAKSP 247

Query: 252 NTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSE 311
           NTDG+HV+ TQ+I I + +I TGDDCISIVSGS+NV+A  ITCGPGHGISIGSLG+G SE
Sbjct: 248 NTDGVHVSNTQYIQISDTIIGTGDDCISIVSGSQNVQATNITCGPGHGISIGSLGSGNSE 307

Query: 312 AEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVTNPIIIDQNYCDQKD 371
           A VSNV ++ AK  G  NGVRIKTWQGG G A NI F N+ M +V  PIIIDQNYCD+ +
Sbjct: 308 AYVSNVTVNEAKIIGAENGVRIKTWQGGSGQASNIKFLNVEMQDVKYPIIIDQNYCDRVE 367

Query: 372 PCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQDINLVHRGKDEKPHE 431
           PC QQ  AVQV NVVY+NI+GTSA++VA+ FDCS + PCEGII+++INLV  G+  KP  
Sbjct: 368 PCIQQFSAVQVKNVVYENIKGTSATKVAIKFDCSTNFPCEGIIMENINLV--GESGKP-- 427

Query: 432 AEASCKNVKLKNRGMVSPQC 445
           +EA+CKNV   N   V+P C
Sbjct: 428 SEATCKNVHFNNAEHVTPHC 442

BLAST of Sed0004377 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 2.3e-121
Identity = 237/443 (53.50%), Postives = 293/443 (66.14%), Query Frame = 0

Query: 11  LLLFLLVVVFELLLTCSSSYDNV---DPPRAISNGDEEHDEYS---------SEFSSML- 70
           + L  L++   +L  C  S   +   DP     NGD   D+ +          EFSS++ 
Sbjct: 1   MALTRLLLPISILWFCFYSSHTILQKDPLLICVNGDPGFDQRAYPTYFGPILDEFSSIMG 60

Query: 71  --KTRLEKTSWSPMSSPEI-----FNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLV 130
              + L    ++P+  PE       +VDD+GA GDG DD++AF++AWK+ACSS  ++ +V
Sbjct: 61  FEPSILSLERFNPVGGPETSPDTDISVDDFGARGDGTDDTKAFEKAWKDACSS-GSVLIV 120

Query: 131 PTERTYHLKPITFPGPCNSPLVFNIEGTIKASPHISDY-EKDRRHWIIFQDISDFTMEGK 190
           P  + Y LK ITF GPC S L   I GTI+AS   SD+   +R+ WI F+DIS+ T+EG 
Sbjct: 121 PENKNYLLKQITFSGPCKSDLRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGG 180

Query: 191 GTINGNGRIWWQNSCKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNN 250
           GT NGNG  WW +SCK  K+LPC  APTA+TF  C NL V  LS ++SQKMHLSF KC +
Sbjct: 181 GTSNGNGETWWDSSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQD 240

Query: 251 VKALNLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGP 310
           V A NL + AP +SPNTDGIH+T TQ I + N +I TGDDCISI SGSK V A  ITCGP
Sbjct: 241 VIASNLMVTAPEHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGP 300

Query: 311 GHGISIGSLGAGKSEAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNV 370
           GHGISIGSLG   SEA VS V++D      T+NG+RIKTWQGG G A+NI F+NIVM+NV
Sbjct: 301 GHGISIGSLGDRNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNV 360

Query: 371 TNPIIIDQNYCDQKDPCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQ 430
           TNPIIIDQ YCD KDPC +Q  AV+VSNV Y NIRGTSAS+VAV FDCSKS PC+G I+ 
Sbjct: 361 TNPIIIDQYYCDSKDPCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVG 420

Query: 431 DINLVHRGKDEKPHEAEASCKNV 433
           +INLV  G      E   SC N+
Sbjct: 421 NINLVGNG----GKETTMSCSNI 438

BLAST of Sed0004377 vs. ExPASy Swiss-Prot
Match: P48978 (Polygalacturonase OS=Malus domestica OX=3750 PE=2 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 3.7e-116
Identity = 211/371 (56.87%), Postives = 266/371 (71.70%), Query Frame = 0

Query: 76  NVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPTERTYHLKPITFPGPCNSPLVFNI 135
           +VDD+GA G+G DD++AF +AWK ACSS+ A+ LV  ++ Y ++PI F GPC S L   I
Sbjct: 100 SVDDFGAKGNGADDTQAFVKAWKAACSSSGAMVLVVPQKNYLVRPIEFSGPCKSQLTLQI 159

Query: 136 EGTIKASPHISDYEKDRRHWIIFQDISDFTMEGKGTINGNGRIWWQNSCKVDKTLPC-TE 195
            GTI+AS   S Y KD  HW+IF ++ +  + G GTINGNG IWW+NSCK+    PC T 
Sbjct: 160 YGTIEASEDRSIY-KDIDHWLIFDNVQNLLVVGPGTINGNGNIWWKNSCKIKPQPPCGTY 219

Query: 196 APTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNVKALNLWIYAPGNSPNTDGIHVTAT 255
           APTAVTF +C NL V+ L+ +++Q++H+ FQ C NV+A  L + AP +SPNTDGIHVT T
Sbjct: 220 APTAVTFNRCNNLVVKNLNIQDAQQIHVIFQNCINVQASCLTVTAPEDSPNTDGIHVTNT 279

Query: 256 QFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVSNVVIDT 315
           Q I I + +I TGDDCISIVSGS+ V+A  ITCGPGHGISIGSLG   SE  VS V ++ 
Sbjct: 280 QNITISSSVIGTGDDCISIVSGSQRVQATDITCGPGHGISIGSLGEDGSEDHVSGVFVNG 339

Query: 316 AKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVTNPIIIDQNYCDQK-DPCTQQADAV 375
           AK SGTSNG+RIKTW+GG G A NI+F+N+ MN+VTNPIIIDQNYCD K   C QQ  AV
Sbjct: 340 AKLSGTSNGLRIKTWKGGSGSATNIVFQNVQMNDVTNPIIIDQNYCDHKTKDCKQQKSAV 399

Query: 376 QVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQDINLVHRGKDEKPHEAEASCKNVK 435
           QV NV+YQNIRGTSAS  A+  +CS+SVPC+GI+LQ + L             A C NV+
Sbjct: 400 QVKNVLYQNIRGTSASGDAITLNCSQSVPCQGIVLQSVQL---------QNGRAECNNVQ 459

Query: 436 LKNRGMVSPQC 445
              +G+VSP+C
Sbjct: 460 PAYKGVVSPRC 460

BLAST of Sed0004377 vs. ExPASy TrEMBL
Match: A0A6J1GMX8 (polygalacturonase QRT2-like OS=Cucurbita moschata OX=3662 GN=LOC111455865 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 9.6e-216
Identity = 372/458 (81.22%), Postives = 406/458 (88.65%), Query Frame = 0

Query: 1   MCRGFLGRSRLLLFLL-----VVVFELLLTCSSSYDNVDPPRAISNG-----DEEHDEYS 60
           MCRG LGR   LL LL     +VVF     CSSSY +VDPP  I  G     D +H  YS
Sbjct: 1   MCRGLLGRRCRLLLLLPFLVAIVVFGFFSVCSSSYADVDPPPLIIRGDGKDRDHQHYGYS 60

Query: 61  SEFSSMLKTRLEKTSWSPMSSPEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLV 120
           S+FSSML TRLEK + SP++S EIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLV
Sbjct: 61  SKFSSMLTTRLEKMASSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLV 120

Query: 121 PTERTYHLKPITFPGPCNSPLVFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEGKG 180
           P +RTYHLKPITF GPCNSP  F IEGTIKASPHISDYEKDRRHWIIF+++S+FT+EGKG
Sbjct: 121 PIDRTYHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLSEFTVEGKG 180

Query: 181 TINGNGRIWWQNSCKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNV 240
           TINGNGR WW NSCKV+KTLPC EAPTAVTFYQCTNLRVEGL FRN+QKMHLSFQKC+NV
Sbjct: 181 TINGNGRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCSNV 240

Query: 241 KALNLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPG 300
           KALNLWIYAPGNSPNTDGIHVTATQ I+I+NCLIMTGDDCISIVSGSKNVRAKGITCGPG
Sbjct: 241 KALNLWIYAPGNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCGPG 300

Query: 301 HGISIGSLGAGKSEAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVT 360
           HGISIGSLGAGKSEAEVSNVV+DTAKF+GTSNGVRIKTWQGGKGYA+NIIF+NIVM+NVT
Sbjct: 301 HGISIGSLGAGKSEAEVSNVVVDTAKFTGTSNGVRIKTWQGGKGYAENIIFQNIVMDNVT 360

Query: 361 NPIIIDQNYCDQKDPCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQD 420
           NPIIIDQNYCDQK+PCTQQA+AVQVSNV+YQNIRGTS S+VAV FDCS+S PC+ I+LQD
Sbjct: 361 NPIIIDQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSESFPCQEILLQD 420

Query: 421 INLVHRGK-DEKPHEAEASCKNVKLKNRGMVSPQCNGR 448
           I+LVHR K D+KP +AEASCKN +LKNRG VSPQC GR
Sbjct: 421 IDLVHRDKADDKPTQAEASCKNARLKNRGRVSPQCPGR 458

BLAST of Sed0004377 vs. ExPASy TrEMBL
Match: A0A6J1JXJ9 (polygalacturonase QRT2-like OS=Cucurbita maxima OX=3661 GN=LOC111488393 PE=3 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 9.0e-214
Identity = 371/460 (80.65%), Postives = 407/460 (88.48%), Query Frame = 0

Query: 1   MCRGFLG---RSRLLLFL----LVVVFELLLTCSSSYDNVDPPRAISNG-----DEEHDE 60
           MCRG LG   R RLLL L     +VVFE    CSSSY +VDPP  I+ G     D+++  
Sbjct: 1   MCRGLLGCRCRCRLLLLLPFLIAIVVFEFFSLCSSSYADVDPPLLINRGDGKDRDQQYHG 60

Query: 61  YSSEFSSMLKTRLEKTSWSPMSSPEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIF 120
           YSS+FSSML TRLEK + SP++S EIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIF
Sbjct: 61  YSSKFSSMLTTRLEKMAPSPVASSEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIF 120

Query: 121 LVPTERTYHLKPITFPGPCNSPLVFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEG 180
           LVP +RTYHLKPITF GPCNSP  F IEGTIKASPHISDYEKDRRHWIIF+++++FT+EG
Sbjct: 121 LVPNDRTYHLKPITFSGPCNSPFAFKIEGTIKASPHISDYEKDRRHWIIFENLTEFTVEG 180

Query: 181 KGTINGNGRIWWQNSCKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCN 240
           KG INGNGR WW NSCKV+KTLPC EAPTAVTFYQCTNLRVEGL FRN+QKMHLSFQKC+
Sbjct: 181 KGIINGNGRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCS 240

Query: 241 NVKALNLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCG 300
           NVKALNLWIYAPGNSPNTDGIHVTATQ I+I+NCLIMTGDDCISIVSGSKNVRAKGITCG
Sbjct: 241 NVKALNLWIYAPGNSPNTDGIHVTATQIILIQNCLIMTGDDCISIVSGSKNVRAKGITCG 300

Query: 301 PGHGISIGSLGAGKSEAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNN 360
           PGHGISIGSLGAGKSEAEVS+VV+DTAKF+GTSNGVRIKTWQGGKGYAQNIIF+NIVM+N
Sbjct: 301 PGHGISIGSLGAGKSEAEVSDVVVDTAKFTGTSNGVRIKTWQGGKGYAQNIIFQNIVMDN 360

Query: 361 VTNPIIIDQNYCDQKDPCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIIL 420
           VTNPIIIDQNYCDQK+PCTQQA+AVQVSNV+YQNIRGTS S+VAV FDCSKS PC  I+L
Sbjct: 361 VTNPIIIDQNYCDQKEPCTQQAEAVQVSNVMYQNIRGTSGSEVAVKFDCSKSFPCLEILL 420

Query: 421 QDINLVHRGK-DEKPHEAEASCKNVKLKNRGMVSPQCNGR 448
           QDI+LVHR K D+K  +AEASCKN +LKNRG VSPQC GR
Sbjct: 421 QDIDLVHRDKTDDKSTQAEASCKNARLKNRGRVSPQCPGR 460

BLAST of Sed0004377 vs. ExPASy TrEMBL
Match: O81246 (Polygalacturonase OS=Cucumis melo OX=3656 GN=MPG3 PE=2 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 3.2e-211
Identity = 370/460 (80.43%), Postives = 404/460 (87.83%), Query Frame = 0

Query: 1   MCRGFLGRS----RLLLFLLVVV--FELLLTCSSSYDNVDPP-RAISNGDEE------HD 60
           MCR  LGR      LL FL VVV  F+   TC  SY +VD P  +IS+GD+E      ++
Sbjct: 1   MCRRLLGRRPCCLSLLTFLFVVVVNFDTFSTCFGSYPDVDNPLPSISSGDKEDYGQHYYE 60

Query: 61  EYSSEFSSMLKTRLEKTSWSPMSSPEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAI 120
             SSEFSSML+TRLEK   SP++SPEIFNVDDYGAMGDGEDD+EAFKE WK+ACSSTNAI
Sbjct: 61  HSSSEFSSMLRTRLEKMVSSPLASPEIFNVDDYGAMGDGEDDTEAFKETWKDACSSTNAI 120

Query: 121 FLVPTERTYHLKPITFPGPCNSPLVFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTME 180
           FLVP +R YHLKPITF GPCNSPL+F IEGT+KASPHISDYEKDRRHWIIFQ++    +E
Sbjct: 121 FLVPCDRVYHLKPITFSGPCNSPLLFQIEGTVKASPHISDYEKDRRHWIIFQNLLGLRVE 180

Query: 181 GKGTINGNGRIWWQNSCKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKC 240
           GKG INGNGR WW NSCKV+KTLPC EAPTAVTFYQCTNLRVEGL FRN+Q+M LSFQ+C
Sbjct: 181 GKGIINGNGRKWWLNSCKVNKTLPCKEAPTAVTFYQCTNLRVEGLRFRNAQQMLLSFQRC 240

Query: 241 NNVKALNLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITC 300
           NNVKALNLWIYAPGNSPNTDGIHVT TQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITC
Sbjct: 241 NNVKALNLWIYAPGNSPNTDGIHVTGTQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITC 300

Query: 301 GPGHGISIGSLGAGKSEAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMN 360
           GPGHGISIGSLGAGKSEAEVSNVV+DTAKFSGTSNGVRIKTWQGGKGYAQNIIF+NIVM+
Sbjct: 301 GPGHGISIGSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMD 360

Query: 361 NVTNPIIIDQNYCDQKDPCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGII 420
           NVTNPIII+QNYCDQK+PCTQQADAV VSNV+YQNIRGTSAS+VAV FDCSKSVPC+GI+
Sbjct: 361 NVTNPIIINQNYCDQKEPCTQQADAVAVSNVMYQNIRGTSASEVAVKFDCSKSVPCQGIL 420

Query: 421 LQDINLVHRGK-DEKPHEAEASCKNVKLKNRGMVSPQCNG 447
           LQDINLVH+GK D+K  +AEASCKNVK KNRG VSPQC G
Sbjct: 421 LQDINLVHKGKNDDKSAQAEASCKNVKWKNRGRVSPQCGG 460

BLAST of Sed0004377 vs. ExPASy TrEMBL
Match: A0A6J1CWK1 (polygalacturonase QRT2 OS=Momordica charantia OX=3673 GN=LOC111015052 PE=3 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 3.0e-209
Identity = 367/454 (80.84%), Postives = 401/454 (88.33%), Query Frame = 0

Query: 1   MCRGFLGRSRLLLFLLVVVFELLLTCSSSYDNVDPPRAISNGD----EEHDEYSSEF--S 60
           MCR    R  LLL LLV V    L  S+S+  VDPP + S+GD    + H EYSSE   S
Sbjct: 1   MCRRLRTRCLLLLPLLVTVLVSDL-YSTSHAAVDPPPSFSSGDGVDHDRHYEYSSELLDS 60

Query: 61  SMLKTRLEKTSWSPMSSPEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLVPTER 120
            +L T+LE+ + SP++SPEIFNVDD+GAMGDGEDDSEAFKEAWKEACSSTN I LVP ER
Sbjct: 61  FVLTTKLERGAPSPVASPEIFNVDDFGAMGDGEDDSEAFKEAWKEACSSTNGILLVPYER 120

Query: 121 TYHLKPITFPGPCNSPLVFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEGKGTING 180
           TYHLKPITF GPCNSPLVF I+GTIKASPH+SDYEKDRRHWI+FQ++ DFT++G GTING
Sbjct: 121 TYHLKPITFSGPCNSPLVFKIDGTIKASPHLSDYEKDRRHWIVFQNMEDFTVKGDGTING 180

Query: 181 NGRIWWQNSCKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNVKALN 240
           NGR WW NSCKV+KTLPC +APTAVTFYQCTNLRVEGL FRN+QKMHLSFQKCNNVKALN
Sbjct: 181 NGRKWWLNSCKVNKTLPCKDAPTAVTFYQCTNLRVEGLRFRNAQKMHLSFQKCNNVKALN 240

Query: 241 LWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGIS 300
           LWIYAPGNSPNTDGIHVT TQFIVI+NCLIMTGDDCISIVSGSKNVRAKGITCGPGHGIS
Sbjct: 241 LWIYAPGNSPNTDGIHVTGTQFIVIENCLIMTGDDCISIVSGSKNVRAKGITCGPGHGIS 300

Query: 301 IGSLGAGKSEAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVTNPII 360
           IGSLGAGKSEAEVSNVV+DTAKFSGTSNGVRIKTWQGGKGYAQNIIF+NIVMNNV+NPII
Sbjct: 301 IGSLGAGKSEAEVSNVVVDTAKFSGTSNGVRIKTWQGGKGYAQNIIFQNIVMNNVSNPII 360

Query: 361 IDQNYCDQKDPCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQDINLV 420
           IDQNYCD+K+ C QQADAVQVSNV Y+NIRGTSAS+VAV FDCSKS+PC+GI+LQD+NLV
Sbjct: 361 IDQNYCDRKERCKQQADAVQVSNVKYRNIRGTSASEVAVKFDCSKSLPCQGILLQDVNLV 420

Query: 421 HRGK-DEKPHEAEASCKNVKLKNRGMVSPQCNGR 448
           HRGK D K  EAEASCKNVKLKNRG VSPQC GR
Sbjct: 421 HRGKGDHKSAEAEASCKNVKLKNRGSVSPQCAGR 453

BLAST of Sed0004377 vs. ExPASy TrEMBL
Match: A0A0A0K6J9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G427620 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 7.6e-181
Identity = 312/384 (81.25%), Postives = 346/384 (90.10%), Query Frame = 0

Query: 1   MCRGFLGRSRLL-----LFLLVVVFELLLTCSSSYDNVDPP-RAISNGDEE----HDEYS 60
           MCR  LGR   L     LF++VV+F++L TCS SY +VD P  +IS+GD+E    H +YS
Sbjct: 1   MCRPLLGRPCCLSLLTFLFVVVVIFDILSTCSGSYPDVDNPLPSISSGDKEVDGQHYKYS 60

Query: 61  SEFSSMLKTRLEKTSWSPMSSPEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLV 120
           SEFSSML+TRLEK + SP++SPEIFNVDDYGAMGDGEDDSEAFKE WK+ACSSTNAIFLV
Sbjct: 61  SEFSSMLRTRLEKMASSPLASPEIFNVDDYGAMGDGEDDSEAFKETWKDACSSTNAIFLV 120

Query: 121 PTERTYHLKPITFPGPCNSPLVFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEGKG 180
           P +R YHLKPITF GPCNSPL+F IEGTIKASPHISDYEKDRRHWIIFQ++    +EGKG
Sbjct: 121 PCDRVYHLKPITFTGPCNSPLLFKIEGTIKASPHISDYEKDRRHWIIFQNLLGLRVEGKG 180

Query: 181 TINGNGRIWWQNSCKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNV 240
            INGNGR WW NSCKV+KTLPC EAPTAVTF+QCTNLRVEGL FRN+Q+MHLSFQKCNNV
Sbjct: 181 IINGNGRKWWLNSCKVNKTLPCKEAPTAVTFFQCTNLRVEGLRFRNAQQMHLSFQKCNNV 240

Query: 241 KALNLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPG 300
           KALNLWI++PGNSPNTDGIHVT TQFIVIKNCLIMTGDDC+SIVSGSKNVRAKGITCGPG
Sbjct: 241 KALNLWIHSPGNSPNTDGIHVTGTQFIVIKNCLIMTGDDCLSIVSGSKNVRAKGITCGPG 300

Query: 301 HGISIGSLGAGKSEAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVT 360
           HGISIGSLGAGK+EAEVSNVV+DTAKFSGT+NGVRIKTWQGGKGYAQNIIF+NIVM+NVT
Sbjct: 301 HGISIGSLGAGKTEAEVSNVVVDTAKFSGTTNGVRIKTWQGGKGYAQNIIFQNIVMDNVT 360

Query: 361 NPIIIDQNYCDQKDPCTQQADAVQ 375
           NPIIIDQNYCDQK+PCTQQADAV+
Sbjct: 361 NPIIIDQNYCDQKEPCTQQADAVE 384

BLAST of Sed0004377 vs. TAIR 10
Match: AT3G07970.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 465.3 bits (1196), Expect = 5.5e-131
Identity = 235/441 (53.29%), Postives = 311/441 (70.52%), Query Frame = 0

Query: 12  LLFLLVVVFELLLTCSSSY------DNVDPPRAISNGDEEHDEYSSEFSSMLKTRLEKTS 71
           ++ L V +   L +C SSY      D           + +H    +  +S LK R     
Sbjct: 5   IIILSVFLLTFLPSCFSSYPFNHRDDLFMSSNVYYETNRQHQHGHNTRNSHLKNR---HG 64

Query: 72  WSPMSSPEIFNVDDYGAMGDGEDDSEAFKEAWKEACSSTNAIFLV-PTERTYHLKPITFP 131
           ++P SSP  FNV+ +GA  +G DDS+AF +AW+ ACSST  +++V P  R Y LK +TF 
Sbjct: 65  YAPRSSPRSFNVNTFGAKANGNDDSKAFMKAWEAACSSTGIVYIVAPKNRDYMLKAVTFS 124

Query: 132 GPCNSPL-VFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEGKGTINGNGRIWWQNS 191
           GPC S L +F I G I+A  + SDY K+RRHWI+F+++++  +EG G I+GNG IWW  S
Sbjct: 125 GPCKSSLIIFKIYGRIEAWENPSDY-KERRHWIVFENVNNLRVEGGGRIDGNGHIWWPKS 184

Query: 192 CKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNVKALNLWIYAPGNS 251
           CK++  LPC  APTAVTF +C NLRV  +   N+Q+MHL+FQ C NVKALNL + +P +S
Sbjct: 185 CKINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADS 244

Query: 252 PNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKS 311
           PNTDGIHV+ TQ I+I++ ++ TGDDCISIVSGS+NVRA GITCGPGHGISIGSLG   S
Sbjct: 245 PNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNS 304

Query: 312 EAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVTNPIIIDQNYCDQK 371
           EA VSNVV++ A   GT+NGVRIKTWQGG G A+NIIF++I+M NVTNPIII+Q+YCD+ 
Sbjct: 305 EAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRV 364

Query: 372 DPCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQDINLVHRGKDEKPH 431
           + C +Q  AVQVSNV+Y+NI+GTS+  +AV F CSK++PC GI +Q++ LV + + +   
Sbjct: 365 EACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQD--- 424

Query: 432 EAEASCKNVKLKNRGMVSPQC 445
            ++ASC NVKL  RG VSP C
Sbjct: 425 VSKASCSNVKLDTRGNVSPLC 438

BLAST of Sed0004377 vs. TAIR 10
Match: AT3G57510.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 409.1 bits (1050), Expect = 4.7e-114
Identity = 224/446 (50.22%), Postives = 298/446 (66.82%), Query Frame = 0

Query: 11  LLLFLLVVVFELLLTCSSSYDNVDPPRAISNGDEEHDEYSSEFSSMLKTRLEKTSWSPMS 70
           L +FL V++  +L  C +   NVD           H++ S E  S+LK   +    S +S
Sbjct: 8   LAVFLCVLL--MLSLCKALSSNVDDGYG-------HEDGSFESDSLLKLNNDDV-LSLIS 67

Query: 71  SPE------IFNVDDYGAMGDGE-DDSEAFKEAWKEACSSTNAI--FLVPTERTYHLKPI 130
           S E        +V ++GA GDG+ DD++AFK+AWK+AC STN +  FLVP  +TY LK  
Sbjct: 68  SDETTLEASTVSVSNFGAKGDGKTDDTQAFKKAWKKAC-STNGVTTFLVPKGKTYLLKST 127

Query: 131 TFPGPCNSPLVFNIEGTIKASPHISDYEKDRRHWIIFQDISDFTMEG--KGTINGNGRIW 190
            F GPC S   F I GT+ AS   SDY KD+ HW+I +D+++ +++G   G INGNG+ W
Sbjct: 128 RFRGPCKSLRNFQILGTLSASTKRSDY-KDKNHWLILEDVNNLSIDGGSTGIINGNGKTW 187

Query: 191 WQNSCKVDKTLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNVKALNLWIYA 250
           WQNSCK+DK+ PCT+APTA+T Y   NL V+ L  +N+Q++ +S +KCN V+  N+ I A
Sbjct: 188 WQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITA 247

Query: 251 PGNSPNTDGIHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLG 310
           PG+SPNTDGIH+T TQ I + N  I TGDDCISI  G++N++   +TCGPGHGISIGSLG
Sbjct: 248 PGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIGSLG 307

Query: 311 AGKSEAEVSNVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVTNPIIIDQNY 370
              S+A VS + +D AKFS + NGVRIKT+QGG G A+NI F+NI M NV NPIIIDQ+Y
Sbjct: 308 DDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPIIIDQDY 367

Query: 371 CDQKDPCTQQADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQDINLVHRGKD 430
           CD KD C  Q  AVQV NVVY+NI GTSA+ VA+  +CS+  PC+GI+L+++        
Sbjct: 368 CD-KDKCEDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENV-------- 427

Query: 431 EKPHEAEASCKNVKLKNRGMVSPQCN 446
            K     ASCKN  +KN+G VSP+C+
Sbjct: 428 -KIKGGTASCKNANVKNQGTVSPKCS 431

BLAST of Sed0004377 vs. TAIR 10
Match: AT2G41850.1 (polygalacturonase abscission zone A. thaliana )

HSP 1 Score: 401.7 bits (1031), Expect = 7.5e-112
Identity = 211/416 (50.72%), Postives = 278/416 (66.83%), Query Frame = 0

Query: 39  ISNGDEEHDEYSSEFSSMLKTRLEKTSW-----SPMSSPEIFNVDDYGAMGDGE-DDSEA 98
           IS    +H     +  S++K R + T       + + +P   +V D+GA GDG+ DD++A
Sbjct: 27  ISPNVYDHSYKRFKSDSLIKRREDITGLRSFVRASLRTPTTVSVSDFGAKGDGKTDDTQA 86

Query: 99  FKEAWKEACSSTNAI-FLVPTERTYHLKPITFPGPCNSPLVFNIEGTIKASPHISDYEKD 158
           F  AWK+ACSS  A+  LVP   TY LK I   GPCNS L   I GT+ AS   SDY KD
Sbjct: 87  FVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSILTVQIFGTLSASQKRSDY-KD 146

Query: 159 RRHWIIFQDISDFTMEG--KGTINGNGRIWWQNSCKVDKTLPCTEAPTAVTFYQCTNLRV 218
              WI+F  +++ +++G   G ++GNG  WWQNSCK +K  PCT+APTA+TFY   +L V
Sbjct: 147 ISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIV 206

Query: 219 EGLSFRNSQKMHLSFQKCNNVKALNLWIYAPGNSPNTDGIHVTATQFIVIKNCLIMTGDD 278
           + L  RN+Q++ +S +KC+NV+  N+ + AP +SPNTDGIH+T TQ I +   +I TGDD
Sbjct: 207 KNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDD 266

Query: 279 CISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVSNVVIDTAKFSGTSNGVRIKTW 338
           CISI SGS+NV+   ITCGPGHGISIGSLG   S+A VS V +D AK SGT NGVRIKT+
Sbjct: 267 CISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTY 326

Query: 339 QGGKGYAQNIIFKNIVMNNVTNPIIIDQNYCDQKDPCTQQADAVQVSNVVYQNIRGTSAS 398
           QGG G A NIIF+NI M+NV NPIIIDQ+YCD K  CT +  AVQV NVVY++I GTSAS
Sbjct: 327 QGGSGTASNIIFQNIQMDNVKNPIIIDQDYCD-KSKCTTEKSAVQVKNVVYRDISGTSAS 386

Query: 399 QVAVNFDCSKSVPCEGIILQDINLVHRGKDEKPHEAEASCKNVKLKNRGMVSPQCN 446
           + A+ F+CSK+ PC+GI+L  +N+            +A+C N  + ++G V PQCN
Sbjct: 387 ENAITFNCSKNYPCQGIVLDRVNI---------KGGKATCTNANVVDKGAVLPQCN 431

BLAST of Sed0004377 vs. TAIR 10
Match: AT1G70500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 361.7 bits (927), Expect = 8.6e-100
Identity = 189/436 (43.35%), Postives = 265/436 (60.78%), Query Frame = 0

Query: 13  LFLLVVVFELLLTCSSSYDNVDPPRAISNGDEEHDEYSSEFSSMLKTRLEKTSW-SPMSS 72
           LF L++V     T    +  +D    + N D   D+   +      T  + +S+ S  S 
Sbjct: 9   LFALLMVASYGATGKMLFKELDILGKLENLDVPEDDIEDDV-----TFFDFSSFTSQYSG 68

Query: 73  PEIFNVDDYGAMGDG-EDDSEAFKEAWKEACSSTNAIFLVPTERTYHLKPITFPGPCNSP 132
             + NVD + A GDG  DD++AF  AW  ACS+ N++ LVP  R+Y +    F GPC   
Sbjct: 69  KNLVNVDSFNASGDGVSDDTQAFIRAWTMACSAPNSVLLVPQGRSYLVNATKFDGPCQEK 128

Query: 133 LVFNIEGTIKASPHISDYE-KDRRHWIIFQDISDFTMEGKGTINGNGRIWWQNSCKVDKT 192
           L+  I+GTI A    S ++ K  R+W+ F  +     +G G I+G+G  WW  SCK +K+
Sbjct: 129 LIIQIDGTIIAPDEPSQWDPKFPRNWLQFSKLQGVVFQGNGVIDGSGTKWWAASCKKNKS 188

Query: 193 LPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNVKALNLWIYAPGNSPNTDGI 252
            PC  APTA+T Y  +N+ V GL+ RNSQ+MHL  Q+   V+   + + +PG+SPNTDGI
Sbjct: 189 NPCVGAPTALTIYSSSNVYVRGLTIRNSQQMHLIIQRSTTVRISRVMVTSPGDSPNTDGI 248

Query: 253 HVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVSN 312
           H+TA+  +V+++  I TGDDC+SIV+GS  ++ K I CGPGHGISIGSLG G S+  V+ 
Sbjct: 249 HITASTDVVVQDSKISTGDDCVSIVNGSAKIKMKRIYCGPGHGISIGSLGQGHSKGTVTA 308

Query: 313 VVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVTNPIIIDQNYCDQKDPCTQQ 372
           VV++TA    T+NG+RIKTWQGG GY + + F+N+VM +V NPIIIDQ YCD    C  Q
Sbjct: 309 VVLETAFLKNTTNGLRIKTWQGGNGYVKGVRFENVVMQDVANPIIIDQFYCDSPSTCQNQ 368

Query: 373 ADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQDINLVHRGKDEKPHEAEASC 432
             AV +S ++Y+NI GT+ S  A+NF CS +VPC  I+L +INL   G D K    EA C
Sbjct: 369 TSAVHISEIMYRNITGTTKSSKAINFKCSDAVPCSHIVLNNINL--EGNDGK---VEAYC 428

Query: 433 KNVKLKNRGMVSPQCN 446
            + +    G+V P  +
Sbjct: 429 NSAEGFGYGVVHPSAD 434

BLAST of Sed0004377 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 357.1 bits (915), Expect = 2.1e-98
Identity = 186/405 (45.93%), Postives = 247/405 (60.99%), Query Frame = 0

Query: 12  LLFLLVVVFELLLTCSSSYDNVDPPRAISNGDEEHDEYSSEFSSMLKTRLEKTSWSPMSS 71
           LL LLVV   L LT +                      ++ F S+L+    ++  +   S
Sbjct: 12  LLLLLVVASSLALTAN----------------------ANSFESLLQLPRRQSRSTRPRS 71

Query: 72  PEIFNVDDYGAMGDG-EDDSEAFKEAWKEACSS-TNAIFLVPTERTYHLKPITFPGPCNS 131
             + +V ++GA G+G  DD++AF +AWK ACSS      LVP   T  L+PI   GPC +
Sbjct: 72  ERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKA 131

Query: 132 PLVFNIEGTIKASPHISDYE-KDRRHWIIFQDISDFTMEGKGTINGNGRIWWQNSCKVDK 191
            L   I GTI A      +E  +RR W+ F  +S  T+EG GT+NG G+ WW+ SCK + 
Sbjct: 132 RLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNH 191

Query: 192 TLPCTEAPTAVTFYQCTNLRVEGLSFRNSQKMHLSFQKCNNVKALNLWIYAPGNSPNTDG 251
           + PC  APTA+TF++C N+RVE L+  +SQ+MH++   C  V    L + AP  SPNTDG
Sbjct: 192 SNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDG 251

Query: 252 IHVTATQFIVIKNCLIMTGDDCISIVSGSKNVRAKGITCGPGHGISIGSLGAGKSEAEVS 311
           IH++ ++ IVI N  + TGDDCISIV  S  +    I CGPGHGISIGSLG  KS  EV 
Sbjct: 252 IHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVR 311

Query: 312 NVVIDTAKFSGTSNGVRIKTWQGGKGYAQNIIFKNIVMNNVTNPIIIDQNYCDQKDPCTQ 371
           ++ +DTA  S T+NGVRIKTWQGG G    IIF+NI MNNV+NPIIIDQ YCD + PC  
Sbjct: 312 DITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCAN 371

Query: 372 QADAVQVSNVVYQNIRGTSASQVAVNFDCSKSVPCEGIILQDINL 414
           Q  A+ + N+ + ++RGTSAS+ A+   CS S PC  I+LQDI+L
Sbjct: 372 QTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDL 394