Sed0004264 (gene) Chayote v1

Overview
NameSed0004264
Typegene
OrganismSechium edule (Chayote v1)
DescriptionATP-dependent 6-phosphofructokinase
LocationLG10: 3474429 .. 3477988 (+)
RNA-Seq ExpressionSed0004264
SyntenySed0004264
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTGCCAAGTTTTTTATTCCACAACTTTACTTTAATTACAACAAGCCACCTCAAACCTCAACACTTTCACAATATTCTCTCAATTTTCCACTCACAAAAATCTCTCCACAATGGCCGCCGCCGCCGCCGCCGCCGAAATCTCCGTCGCAAAACTGCCCCACCTGACCGACACGTTCCCGGAGCTCCGAACCCGAACCCGACCCAACCCGCTTCACAGCAACCAGTTCTTCCACCCAATGGACGGCTTCTACGTGGCCCCCACCGACGTGGTCCTCCGCCAGATCGCCTTCGACCTCACCGCCTTCCCCGGCCACGTGGCGTACCACCGGGCGGGCCCTCGTCGGTCGGTTTATTTCGACCCGGACGACGTCCGGGCCGCGATTGTCACGTGCGGGGGGCTGTGCCCGGGGCTCAACACGGTGATCCGAGAGCTGGTGGTGGGGCTGTGGGACCTCTACGGGGTGCGCCGGATTGATGGCATTGTGGCCGGTTATAGAGGGTTTTACTCGACCGATTCGGTCGAGTTGAACCCGGTCATGGTTCGCGATTGGCACAAGAGGGGCGGTTCGGCTCTTGAAACCTCCCGTGGTGGATTTGATCTCAGGAAAATCGTTGATGCTTTGCAAGATCGTGGTTTCAATCAGGTTGGTTCTTGCGCTCTTTTCAACTTTATTTGTTTTTTTTTTCCTTGGTAGCGGTGGATGAGATTTATATAAAAAAAAAATAGTACATATTAAGGGAGAGGGATAAATACCGATTCGTGGGACCACATATGTTATGACCGTAGAGATGTCTACGGGGTGGGGATGGGGTGACTTTACCGCCCCTTCCTCACTACCCATTTCGAAAGTAAGCTGAAAATTTTGTAGAGAATGGGGCGGGCATTCACTGTTGTAAATAACGGGGATTGAGTTTTCAACAGAATTATTTCCCATTTAACTCTTCATTTCCTATTTTTACTTTTTTCAAACTTTTTTTTTATTTTTAACTTAATCGACTAAATCTTAGATAATTGCTTTAATATTCAAAGAAATGTTTATTTATTTTGAATTAAATAATTAATTAAAGAATCCAAATAAATATTTTTACTACATTACTTATATAAATGGGGATGGGAATAAATTTTCCATGCCCATCCCCATTCAGTTCGTGGGAAATTTTTCATCCCCACTTACTCCTCTCTATTCAGGGATTTACTGCCCTCATAGGGGCTGATCCCCATGGGTCCAGTTTCGTGAGAAAAATGAACATCTCCAAATACTAGTTTTGAATTGGTTTACGACAAAACAATGAAACGTCACTGAACCATTTGGTAGATTTATATAATTTTTTAAAACACTGATAAACATTCTTTTTTTTTTGGATAAGAAACAATTTCATTAACATATGAAATTAAGAGTTACAAGGATAAGGGGGAATTACAAAAGAACTCTCCTTACTAATAGTAAGACTAGTAAAACTATAATCATGAAATAGAGGAGAGAGTTTACACCAAGAAATAACAGTAACAAACACCGATAAACATTCAATGACGTGATATTTAAATTCGGTAAGTTTTAAAGCTTTAGTATAGTTTAATTGGCCAACTAGAGCACTAGAAATCAAGAGGGTGATGGGTGAAAGAGTATAGTTTAAATGGGAAAGATTGTTCAAATCCTCTCCTTTTGTCTTGATCTCTTCGACATTTTCTTATATGAGAACTTTGCACAGAATTTTTTGAGTGAAAAATTGGTGGAATTTTGGCTTTTAACTTTTAATTTTATTACTCATTCATTAATGAATTTGTAGCATGACTTTTCTAACTAGGGTAGAGTGATCCAGATATTTTGTAATTCCTAGGAAATATGAGGTCGTCTCACTTCATAACCTACGAAATGGTGTCACAAGTCAATTCCAAATTGGATTTTAATTATTCCTTGATTAAACTAAATATCAAGTTTAATGACTATGATATTATATTAAATTATTATGAAGTTTCGTCTAAAAACTAATTGTTAATAAATAAGTAATCTACCTATTTTATAAGTACCTGAACTCGTAACAACAAGAGATCACATTAAATCAGTAATCTTGAAAAATTGCTATGTTTACCGAATGTGTCAGTAGCGTAATGGTTTCATGTTTGGTTGTAAACTAGTTCAACACCAGCTCACAAGTTCAATCTTGAAAATCGGTATTTGTCTCCCTCCCCTGATATGTATTCCAAAAAAAAAACTTCTAATTTATAGGATCTTGGTGTTGATGACAATTTACTCTAATTTTGCAACAATTTAGTGCTCTAGTTGATGATCTGGGTGTTGTAAAGCTAAACATGGCATATGTTTTCAAAAAAAGAAAAAGAAAGCTAAACGTGGCGGGAAAACGAGACTTATGTCTTACATTTCAGCAAAAAAAGGAGAAAATTATCGTTGTCAAGCAGTTGTAGCAAGCCATTAGGAGTTTTAATGGAGATTCCTTGCAAATGAATTAGGTCTACATAATCGGAGGAGATGGCACAATGCGGGGCGCCGTCAAGATATTCGACGAAATACGTCGTCGAAACCTTCAAATTTCCATCACCGGAATCCCCAAAACCGTCGACAACGACATCGGAATCATCGATCGATCATTCGGATTCCAAACCGCCGTCGAAATGGCGCAACAAGCAATCAACGCCGCTCACGTAGAGGCCGAGAGCGCGGTGAACGGCATAGGTTTAGTGAAATTGATGGGCCGAAGCACCGGTCACATTGCTCTTCACGCCACATTGAGCAGCCGAGACGTCGATTGCTGTTTGATTCCTGAAATGGAGTTCTATCTGGAAGGCGATGGCGGTTTGTTTCCGTTTCTCGATCGGAAGCTGAAGGAGAATGGACATGCGGTTCTGGTGGTTGCGGAGGGAGCAGGGCAAGATATGATACCGAGATCTGAGGCGCAAATGGAAGAGAGGGATGAATCGGGGAATCCAGTGTTCTTGGACGTCGGCGGGTGGTTGAGGACGGAGCTTAAGAATTGGTGGGCGAGGACGCATCCTGGTGAGTTATTTACTGTAAAATATATAGATCCTACTTATATGATTCGTGCTGTGCCTGCTAATGCTACAGATAACTTGTATTGTACATTGCTTGCTCATTCTGCTATTCATGGGGTAATGGCTGGTTATACTGGATTTGTTCCTGGTCCTATTAATGGAAACTATTCCTATATTCCATTGAAAGAGGTGGCTGAAGCAAAGAATGAAGTTGATGCAAGAGATCATAAATGGGCATGGGTTAGATCTGTCACTAATCAACCTGATTTTGTGATAATTAGTTAACTGTTTGATTTGAGAAATCAGAGTTCATGTGGGGTTTTTAGTTTAAATAAATTTTAGGATTGTGTGTATATTTGTAGCTTAACCTTAACTTTTGAAATCGAAGTACTTGTTTGGTTGCTCAAATTTTTTTCAGGAAATGATATGGGCATTGCATAATACACTTTTGTTTTGTACACACAAATCTCAATGATCAAACCAGAACACAATCTGAGATATGATTTATCTAGCAATACAGAAAAAGTAGAATTACGGAACCGTCGACAAC

mRNA sequence

TCTGCCAAGTTTTTTATTCCACAACTTTACTTTAATTACAACAAGCCACCTCAAACCTCAACACTTTCACAATATTCTCTCAATTTTCCACTCACAAAAATCTCTCCACAATGGCCGCCGCCGCCGCCGCCGCCGAAATCTCCGTCGCAAAACTGCCCCACCTGACCGACACGTTCCCGGAGCTCCGAACCCGAACCCGACCCAACCCGCTTCACAGCAACCAGTTCTTCCACCCAATGGACGGCTTCTACGTGGCCCCCACCGACGTGGTCCTCCGCCAGATCGCCTTCGACCTCACCGCCTTCCCCGGCCACGTGGCGTACCACCGGGCGGGCCCTCGTCGGTCGGTTTATTTCGACCCGGACGACGTCCGGGCCGCGATTGTCACGTGCGGGGGGCTGTGCCCGGGGCTCAACACGGTGATCCGAGAGCTGGTGGTGGGGCTGTGGGACCTCTACGGGGTGCGCCGGATTGATGGCATTGTGGCCGGTTATAGAGGGTTTTACTCGACCGATTCGGTCGAGTTGAACCCGGTCATGGTTCGCGATTGGCACAAGAGGGGCGGTTCGGCTCTTGAAACCTCCCGTGGTGGATTTGATCTCAGGAAAATCGTTGATGCTTTGCAAGATCGTGGTTTCAATCAGGTCTACATAATCGGAGGAGATGGCACAATGCGGGGCGCCGTCAAGATATTCGACGAAATACGTCGTCGAAACCTTCAAATTTCCATCACCGGAATCCCCAAAACCGTCGACAACGACATCGGAATCATCGATCGATCATTCGGATTCCAAACCGCCGTCGAAATGGCGCAACAAGCAATCAACGCCGCTCACGTAGAGGCCGAGAGCGCGGTGAACGGCATAGGTTTAGTGAAATTGATGGGCCGAAGCACCGGTCACATTGCTCTTCACGCCACATTGAGCAGCCGAGACGTCGATTGCTGTTTGATTCCTGAAATGGAGTTCTATCTGGAAGGCGATGGCGGTTTGTTTCCGTTTCTCGATCGGAAGCTGAAGGAGAATGGACATGCGGTTCTGGTGGTTGCGGAGGGAGCAGGGCAAGATATGATACCGAGATCTGAGGCGCAAATGGAAGAGAGGGATGAATCGGGGAATCCAGTGTTCTTGGACGTCGGCGGGTGGTTGAGGACGGAGCTTAAGAATTGGTGGGCGAGGACGCATCCTGGTGAGTTATTTACTGTAAAATATATAGATCCTACTTATATGATTCGTGCTGTGCCTGCTAATGCTACAGATAACTTGTATTGTACATTGCTTGCTCATTCTGCTATTCATGGGGTAATGGCTGGTTATACTGGATTTGTTCCTGGTCCTATTAATGGAAACTATTCCTATATTCCATTGAAAGAGGTGGCTGAAGCAAAGAATGAAGTTGATGCAAGAGATCATAAATGGGCATGGGTTAGATCTGTCACTAATCAACCTGATTTTGTGATAATTAGTTAACTGTTTGATTTGAGAAATCAGAGTTCATGTGGGGTTTTTAGTTTAAATAAATTTTAGGATTGTGTGTATATTTGTAGCTTAACCTTAACTTTTGAAATCGAAGTACTTGTTTGGTTGCTCAAATTTTTTTCAGGAAATGATATGGGCATTGCATAATACACTTTTGTTTTGTACACACAAATCTCAATGATCAAACCAGAACACAATCTGAGATATGATTTATCTAGCAATACAGAAAAAGTAGAATTACGGAACCGTCGACAAC

Coding sequence (CDS)

ATGGCCGCCGCCGCCGCCGCCGCCGAAATCTCCGTCGCAAAACTGCCCCACCTGACCGACACGTTCCCGGAGCTCCGAACCCGAACCCGACCCAACCCGCTTCACAGCAACCAGTTCTTCCACCCAATGGACGGCTTCTACGTGGCCCCCACCGACGTGGTCCTCCGCCAGATCGCCTTCGACCTCACCGCCTTCCCCGGCCACGTGGCGTACCACCGGGCGGGCCCTCGTCGGTCGGTTTATTTCGACCCGGACGACGTCCGGGCCGCGATTGTCACGTGCGGGGGGCTGTGCCCGGGGCTCAACACGGTGATCCGAGAGCTGGTGGTGGGGCTGTGGGACCTCTACGGGGTGCGCCGGATTGATGGCATTGTGGCCGGTTATAGAGGGTTTTACTCGACCGATTCGGTCGAGTTGAACCCGGTCATGGTTCGCGATTGGCACAAGAGGGGCGGTTCGGCTCTTGAAACCTCCCGTGGTGGATTTGATCTCAGGAAAATCGTTGATGCTTTGCAAGATCGTGGTTTCAATCAGGTCTACATAATCGGAGGAGATGGCACAATGCGGGGCGCCGTCAAGATATTCGACGAAATACGTCGTCGAAACCTTCAAATTTCCATCACCGGAATCCCCAAAACCGTCGACAACGACATCGGAATCATCGATCGATCATTCGGATTCCAAACCGCCGTCGAAATGGCGCAACAAGCAATCAACGCCGCTCACGTAGAGGCCGAGAGCGCGGTGAACGGCATAGGTTTAGTGAAATTGATGGGCCGAAGCACCGGTCACATTGCTCTTCACGCCACATTGAGCAGCCGAGACGTCGATTGCTGTTTGATTCCTGAAATGGAGTTCTATCTGGAAGGCGATGGCGGTTTGTTTCCGTTTCTCGATCGGAAGCTGAAGGAGAATGGACATGCGGTTCTGGTGGTTGCGGAGGGAGCAGGGCAAGATATGATACCGAGATCTGAGGCGCAAATGGAAGAGAGGGATGAATCGGGGAATCCAGTGTTCTTGGACGTCGGCGGGTGGTTGAGGACGGAGCTTAAGAATTGGTGGGCGAGGACGCATCCTGGTGAGTTATTTACTGTAAAATATATAGATCCTACTTATATGATTCGTGCTGTGCCTGCTAATGCTACAGATAACTTGTATTGTACATTGCTTGCTCATTCTGCTATTCATGGGGTAATGGCTGGTTATACTGGATTTGTTCCTGGTCCTATTAATGGAAACTATTCCTATATTCCATTGAAAGAGGTGGCTGAAGCAAAGAATGAAGTTGATGCAAGAGATCATAAATGGGCATGGGTTAGATCTGTCACTAATCAACCTGATTTTGTGATAATTAGTTAA

Protein sequence

MAAAAAAAEISVAKLPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLTAFPGHVAYHRAGPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIVAGYRGFYSTDSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGDGTMRGAVKIFDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKENGHAVLVVAEGAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTVKYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEAKNEVDARDHKWAWVRSVTNQPDFVIIS
Homology
BLAST of Sed0004264 vs. NCBI nr
Match: XP_038881531.1 (ATP-dependent 6-phosphofructokinase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 808.9 bits (2088), Expect = 2.2e-230
Identity = 385/442 (87.10%), Postives = 413/442 (93.44%), Query Frame = 0

Query: 10  ISVAKLPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLTA--FPG 69
           I+VA LPHLTD FP+L TRT  NPL +NQFFHP+DGFY + TDVVLR+IAFDL+    P 
Sbjct: 13  IAVADLPHLTDDFPDLETRT--NPLQTNQFFHPIDGFYASSTDVVLRRIAFDLSTKESPA 72

Query: 70  HVAYHRAGPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIVAG 129
           HVAYHRAGPRRSVYF+P+ VRAAIVTCGGLCPG+NTVIRELVVGLW+LYGVRRI GI+AG
Sbjct: 73  HVAYHRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRRIYGILAG 132

Query: 130 YRGFYSTDSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGDGT 189
           YRGFYST+ VELN  +V +WHKRGG+AL TSRGGFDLRKIVD +Q  GFNQVYIIGGDGT
Sbjct: 133 YRGFYSTEPVELNLKLVDNWHKRGGTALRTSRGGFDLRKIVDTIQHHGFNQVYIIGGDGT 192

Query: 190 MRGAVKIFDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAES 249
           MRGAVKIFDEIRRRNLQ+++TGIPKTVDNDIGI+DRSFGFQTAVEMAQQAI+AAHVEAES
Sbjct: 193 MRGAVKIFDEIRRRNLQVAVTGIPKTVDNDIGIVDRSFGFQTAVEMAQQAISAAHVEAES 252

Query: 250 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKENGH 309
           AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEGDGGLFPFL+R+LKENGH
Sbjct: 253 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGDGGLFPFLERRLKENGH 312

Query: 310 AVLVVAEGAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTVKY 369
           AVLVVAEGAGQ MIPR+EA+ EERDESGNPVFLDVGGWL+TELKNWWARTHPGELFTVKY
Sbjct: 313 AVLVVAEGAGQSMIPRTEAEKEERDESGNPVFLDVGGWLKTELKNWWARTHPGELFTVKY 372

Query: 370 IDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEAKN 429
           IDPTYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPINGNYSYIPLKEVAEAKN
Sbjct: 373 IDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKN 432

Query: 430 EVDARDHKWAWVRSVTNQPDFV 450
           EV+ RDHKWAWVRSVTNQPDFV
Sbjct: 433 EVNTRDHKWAWVRSVTNQPDFV 452

BLAST of Sed0004264 vs. NCBI nr
Match: XP_023544772.1 (ATP-dependent 6-phosphofructokinase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 805.4 bits (2079), Expect = 2.4e-229
Identity = 388/456 (85.09%), Postives = 422/456 (92.54%), Query Frame = 0

Query: 3   AAAAAAE-------ISVAKLPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVL 62
           A AAAAE       I+VA LPHLTD FP L++RT  NPL +NQFFHP+DGFY   TDVVL
Sbjct: 2   ATAAAAEIADRHPPIAVASLPHLTDHFPTLQSRT--NPLQNNQFFHPIDGFYATSTDVVL 61

Query: 63  RQIAFDLT--AFPGHVAYHRAGPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLW 122
           R+IAFDL+    PGHVAY+RAGPRRSVYF+P+ VRAAIVTCGGLCPG+NTVIRELVVGLW
Sbjct: 62  RRIAFDLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLW 121

Query: 123 DLYGVRRIDGIVAGYRGFYSTDSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQD 182
           +LYGVR+I GIVAGYRGFYS++ VEL+  +V DWHKRGG+AL TSRGGFDLR+IVDA+QD
Sbjct: 122 ELYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQD 181

Query: 183 RGFNQVYIIGGDGTMRGAVKIFDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEM 242
           RGFNQVYIIGGDGTMRGAVKIF+E+ RRNLQ+++TGIPKTVDNDIGIIDRSFGFQTAVEM
Sbjct: 182 RGFNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEM 241

Query: 243 AQQAINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGG 302
           AQQA++AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGG
Sbjct: 242 AQQAVSAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGG 301

Query: 303 LFPFLDRKLKENGHAVLVVAEGAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNW 362
           LFPFL+R+LKENGHAVLVVAEGAGQD IPR+EAQ EERDESGNPVFLDVGGWL+TELK+W
Sbjct: 302 LFPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDW 361

Query: 363 WARTHPGELFTVKYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGN 422
           WARTHPGELFTVKYIDPTYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPINGN
Sbjct: 362 WARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGN 421

Query: 423 YSYIPLKEVAEAKNEVDARDHKWAWVRSVTNQPDFV 450
           YSYIPLK+VAEAKNEV+ RDHKWAWVRSVTNQPDFV
Sbjct: 422 YSYIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFV 455

BLAST of Sed0004264 vs. NCBI nr
Match: KAG6603845.1 (ATP-dependent 6-phosphofructokinase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034027.1 ATP-dependent 6-phosphofructokinase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 804.3 bits (2076), Expect = 5.4e-229
Identity = 388/456 (85.09%), Postives = 421/456 (92.32%), Query Frame = 0

Query: 3   AAAAAAE-------ISVAKLPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVL 62
           A  AAAE       I+VA LPHLTD FP L++RT  NPL +NQFFHP+DGFY   TDVVL
Sbjct: 2   ATTAAAEIADRHPPIAVASLPHLTDHFPTLQSRT--NPLQNNQFFHPIDGFYATSTDVVL 61

Query: 63  RQIAFDLT--AFPGHVAYHRAGPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLW 122
           R+IAFDL+    PGHVAY+RAGPRRSVYF+P+ VRAAIVTCGGLCPG+NTVIRELVVGLW
Sbjct: 62  RRIAFDLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLW 121

Query: 123 DLYGVRRIDGIVAGYRGFYSTDSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQD 182
           +LYGVR+I GIVAGYRGFYS++ VEL+  +V DWHKRGG+AL TSRGGFDLR+IVDA+QD
Sbjct: 122 ELYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQD 181

Query: 183 RGFNQVYIIGGDGTMRGAVKIFDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEM 242
           RGFNQVYIIGGDGTMRGAVKIF+E+ RRNLQ+++TGIPKTVDNDIGIIDRSFGFQTAVEM
Sbjct: 182 RGFNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEM 241

Query: 243 AQQAINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGG 302
           AQQAI+AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGG
Sbjct: 242 AQQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGG 301

Query: 303 LFPFLDRKLKENGHAVLVVAEGAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNW 362
           LFPFL+R+LKENGHAVLVVAEGAGQD IPR+EAQ EERDESGNPVFLDVGGWL+TELK+W
Sbjct: 302 LFPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDW 361

Query: 363 WARTHPGELFTVKYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGN 422
           WARTHPGELFTVKYIDPTYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPINGN
Sbjct: 362 WARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGN 421

Query: 423 YSYIPLKEVAEAKNEVDARDHKWAWVRSVTNQPDFV 450
           YSYIPLK+VAEAKNEV+ RDHKWAWVRSVTNQPDFV
Sbjct: 422 YSYIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFV 455

BLAST of Sed0004264 vs. NCBI nr
Match: XP_022977934.1 (ATP-dependent 6-phosphofructokinase 2 [Cucurbita maxima])

HSP 1 Score: 802.7 bits (2072), Expect = 1.6e-228
Identity = 388/458 (84.72%), Postives = 421/458 (91.92%), Query Frame = 0

Query: 1   MAAAAAAAEIS-------VAKLPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDV 60
           MA A AAAEIS       VA LPHLTD FP L++RT  NPL +NQFFHP+DGFY   TDV
Sbjct: 1   MATATAAAEISDRHPPIAVASLPHLTDHFPTLQSRT--NPLQNNQFFHPIDGFYATSTDV 60

Query: 61  VLRQIAFDLT--AFPGHVAYHRAGPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVG 120
           VLR+IAFDL+    P HVAY+RAGPRRSVYF+P+ VRAAIVTCGGLCPG+NTVIRELVVG
Sbjct: 61  VLRRIAFDLSRKPSPAHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVG 120

Query: 121 LWDLYGVRRIDGIVAGYRGFYSTDSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDAL 180
           LW+LYGVR+I GIVAGYRGFYS++ VEL+  +V DWHKRGG+AL TSRGGFDLR+IVDA+
Sbjct: 121 LWELYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAI 180

Query: 181 QDRGFNQVYIIGGDGTMRGAVKIFDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAV 240
           QDRGFNQVYIIGGDGTMRGAVKIF+E+ RR LQ+++TGIPKTVDNDIGIIDRSFGFQTAV
Sbjct: 181 QDRGFNQVYIIGGDGTMRGAVKIFEEVCRRELQVAVTGIPKTVDNDIGIIDRSFGFQTAV 240

Query: 241 EMAQQAINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGD 300
           EMAQQAI+AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGD
Sbjct: 241 EMAQQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGD 300

Query: 301 GGLFPFLDRKLKENGHAVLVVAEGAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELK 360
           GGLFPFL+R+LKENGHAVLVVAEGAGQD IPR+EAQ EERDESGNPVFLDVGGWL+TELK
Sbjct: 301 GGLFPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELK 360

Query: 361 NWWARTHPGELFTVKYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPIN 420
           +WWARTHPGELFTVKYIDPTYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPIN
Sbjct: 361 DWWARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPIN 420

Query: 421 GNYSYIPLKEVAEAKNEVDARDHKWAWVRSVTNQPDFV 450
           GNYSYIPLK+VAEAKNEV+ +DHKWAWVRSVTNQPDFV
Sbjct: 421 GNYSYIPLKDVAEAKNEVNTKDHKWAWVRSVTNQPDFV 456

BLAST of Sed0004264 vs. NCBI nr
Match: XP_022950529.1 (ATP-dependent 6-phosphofructokinase 2 [Cucurbita moschata])

HSP 1 Score: 802.4 bits (2071), Expect = 2.1e-228
Identity = 387/455 (85.05%), Postives = 420/455 (92.31%), Query Frame = 0

Query: 4   AAAAAE-------ISVAKLPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLR 63
           A AAAE       I+VA LPHLTD FP L++RT  NPL +NQFFHP+DGFY   TDVVLR
Sbjct: 2   ATAAAEIADRHPPIAVASLPHLTDHFPTLQSRT--NPLQNNQFFHPIDGFYATSTDVVLR 61

Query: 64  QIAFDLT--AFPGHVAYHRAGPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWD 123
           +IAFDL+    P HVAY+RAGPRRSVYF+P+ VRAAIVTCGGLCPG+NTVIRELVVGLW+
Sbjct: 62  RIAFDLSRKPSPAHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWE 121

Query: 124 LYGVRRIDGIVAGYRGFYSTDSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDR 183
           LYGVR+I GIVAGYRGFYS++ VEL+  +V DWHKRGG+AL TSRGGFDLR+IVDA+QDR
Sbjct: 122 LYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDR 181

Query: 184 GFNQVYIIGGDGTMRGAVKIFDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMA 243
           GFNQVYIIGGDGTMRGAVKIF+E+ RRNLQ+++TGIPKTVDNDIGIIDRSFGFQTAVEMA
Sbjct: 182 GFNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMA 241

Query: 244 QQAINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGL 303
           QQAI+AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGL
Sbjct: 242 QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGL 301

Query: 304 FPFLDRKLKENGHAVLVVAEGAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWW 363
           FPFL+R+LKENGHAVLVVAEGAGQD IPR+EAQ EERDESGNPVFLDVGGWL+TELK+WW
Sbjct: 302 FPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWW 361

Query: 364 ARTHPGELFTVKYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNY 423
           ARTHPGELFTVKYIDPTYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPINGNY
Sbjct: 362 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNY 421

Query: 424 SYIPLKEVAEAKNEVDARDHKWAWVRSVTNQPDFV 450
           SYIPLK+VAEAKNEV+ RDHKWAWVRSVTNQPDFV
Sbjct: 422 SYIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFV 454

BLAST of Sed0004264 vs. ExPASy Swiss-Prot
Match: Q9FIK0 (ATP-dependent 6-phosphofructokinase 2 OS=Arabidopsis thaliana OX=3702 GN=PFK2 PE=1 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 4.5e-202
Identity = 335/443 (75.62%), Postives = 380/443 (85.78%), Query Frame = 0

Query: 7   AAEISVAKLPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLTAF- 66
           AAE S+ KLP L+        R R NPL  N +FHP +GFY+ P+DV+L Q+A+D +A  
Sbjct: 2   AAETSIRKLPSLSG------LRHRRNPLEDNPYFHPSNGFYITPSDVILAQVAYDHSAHS 61

Query: 67  PGHVAYHRAGPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIV 126
              VAYHRAGPRR + ++P  V+AAIVTCGGLCPG+NTVIRELVVGLW+LYGVR I GI 
Sbjct: 62  QSRVAYHRAGPRREIMYEPSAVKAAIVTCGGLCPGMNTVIRELVVGLWELYGVREIYGIP 121

Query: 127 AGYRGFYSTDSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGD 186
           AGYRGFYS  +V+L+P  V DWHK+GG+ L TSRGGF L+KIVDA+   G+NQVYIIGGD
Sbjct: 122 AGYRGFYSMKAVKLDPKAVHDWHKKGGTVLATSRGGFHLQKIVDAIHLNGYNQVYIIGGD 181

Query: 187 GTMRGAVKIFDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEA 246
           GTMRGAV+IF EI  R L++ IT IPKTVDND+GIIDRSFGFQTAVEMAQ+AI+AAHVEA
Sbjct: 182 GTMRGAVEIFKEISLRKLEVGITVIPKTVDNDVGIIDRSFGFQTAVEMAQEAISAAHVEA 241

Query: 247 ESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKEN 306
           ESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM+FYLEG GGLF FL+++LKE 
Sbjct: 242 ESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMDFYLEGKGGLFEFLEKRLKER 301

Query: 307 GHAVLVVAEGAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTV 366
           GHAVLVVAEGAGQ+MIPR+E+Q +ERDESGN VFLDVG W ++ LK WW R HP ELFTV
Sbjct: 302 GHAVLVVAEGAGQEMIPRNESQKQERDESGNAVFLDVGVWFKSVLKAWWEREHPDELFTV 361

Query: 367 KYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEA 426
           KYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNY+YIPL+EVA+ 
Sbjct: 362 KYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYAYIPLEEVAQT 421

Query: 427 KNEVDARDHKWAWVRSVTNQPDF 449
           KN+V+ RDHKWAWVRSVTNQPDF
Sbjct: 422 KNQVNTRDHKWAWVRSVTNQPDF 438

BLAST of Sed0004264 vs. ExPASy Swiss-Prot
Match: Q94AA4 (ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE=1 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 3.0e-145
Identity = 252/435 (57.93%), Postives = 320/435 (73.56%), Query Frame = 0

Query: 15  LPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLTAFPGHVAYHRA 74
           +PHL+D  P L   T PNPL  N  +  +  ++V   D V ++I       P  + + RA
Sbjct: 23  VPHLSDYLPGL--PTYPNPLQDNPAYSVVKQYFVDADDSVPQKIVVHKDG-PRGIHFRRA 82

Query: 75  GPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIVAGYRGFYST 134
           GPR+ VYF+ D+V A IVTCGGLCPGLNTVIRE+V  L  +YGV+RI GI  GYRGFY+ 
Sbjct: 83  GPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGGYRGFYAK 142

Query: 135 DSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGDGTMRGAVKI 194
           ++V L+  +V D HKRGG+ L TSRGG D  KIVD++QDRG NQVYIIGGDGT RGA  I
Sbjct: 143 NTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGINQVYIIGGDGTQRGASVI 202

Query: 195 FDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIGL 254
           F+EIRRR L++++ GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVEAES  NGIG+
Sbjct: 203 FEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESIENGIGV 262

Query: 255 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKENGHAVLVVAE 314
           VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG+GGLF +++++LKE+GH VLV+AE
Sbjct: 263 VKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGHMVLVIAE 322

Query: 315 GAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTVKYIDPTYMI 374
           GAGQD++ +S   M  +D SGN +  DVG WL   +K+ +       +  +KYIDPTYMI
Sbjct: 323 GAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDHF--NQKKMVMNLKYIDPTYMI 382

Query: 375 RAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEAKNEVDARDH 434
           RAVP+NA+DN+YCTLLA SA+HG MAGYTG++ G +NG  +YIP   + E +N V   D 
Sbjct: 383 RAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTYIPFYRITEKQNHVVITDR 442

Query: 435 KWAWVRSVTNQPDFV 450
            WA + S TNQP F+
Sbjct: 443 MWARLLSSTNQPSFL 452

BLAST of Sed0004264 vs. ExPASy Swiss-Prot
Match: Q9FKG3 (ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PFK4 PE=1 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.1e-144
Identity = 246/435 (56.55%), Postives = 318/435 (73.10%), Query Frame = 0

Query: 15  LPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLTAFPGHVAYHRA 74
           +PHLT   P+L   + PNPL  +Q +  +   +V+  DVV + I     +  G V + RA
Sbjct: 74  VPHLTKFLPDL--PSYPNPLKESQAYAIVKRTFVSSEDVVAQNIVVQKGSKRG-VHFRRA 133

Query: 75  GPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIVAGYRGFYST 134
           GPR  VYF  D+V+A IVTCGGLCPG+NTVIRE+V GL ++YGV  I GI  GYRGFYS 
Sbjct: 134 GPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGGYRGFYSK 193

Query: 135 DSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGDGTMRGAVKI 194
           +++ L P +V D HKRGG+ L+TSRGG D  KIVD +QDRG NQVYIIGG GT +GA KI
Sbjct: 194 NTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGTQKGAEKI 253

Query: 195 FDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIGL 254
           ++E+ RR LQ++++GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVE ES  NG+G+
Sbjct: 254 YEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVESVENGVGI 313

Query: 255 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKENGHAVLVVAE 314
           VKLMGR +G IA+ ATL++RDVDCCLIPE  F+LEG GGLF F++ +LKEN H V+V+AE
Sbjct: 314 VKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRHMVIVIAE 373

Query: 315 GAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTVKYIDPTYMI 374
           GAGQD + +S    E +D SGN + LDVG WL  ++K+ +       +  +KYIDPTYMI
Sbjct: 374 GAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRK-MMINMKYIDPTYMI 433

Query: 375 RAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEAKNEVDARDH 434
           RA+P+NA+DN+YCTLLA SA+HG MAGY+GF  GP+N  ++YIP+ +V E  N V   D 
Sbjct: 434 RAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTNTVKLTDR 493

Query: 435 KWAWVRSVTNQPDFV 450
            WA + + TNQP F+
Sbjct: 494 MWARLLASTNQPSFL 504

BLAST of Sed0004264 vs. ExPASy Swiss-Prot
Match: Q9M076 (ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE=1 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 4.3e-144
Identity = 250/435 (57.47%), Postives = 320/435 (73.56%), Query Frame = 0

Query: 15  LPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLTAFPGHVAYHRA 74
           +PHL+D   +L   T PNPL SN  +  +  ++V   D V  +I     + P    + RA
Sbjct: 24  VPHLSDYILDL--PTYPNPLQSNAAYSVVRQYFVDEDDTVQEKIVVHKDS-PRGTHFRRA 83

Query: 75  GPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIVAGYRGFYST 134
           GPR+ VYF P DVRA IVTCGGLCPGLNTVIRE+V GL  +YGV  + G+  G+RGFYS 
Sbjct: 84  GPRQKVYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHFMYGVTEVIGVDCGFRGFYSK 143

Query: 135 DSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGDGTMRGAVKI 194
           ++V L P  V D HKRGG+ L TSRGG D  KIVD +QDR  NQVYIIGGDGT +GA  I
Sbjct: 144 NTVALTPKTVSDIHKRGGTILGTSRGGHDTSKIVDNIQDREINQVYIIGGDGTQKGANAI 203

Query: 195 FDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIGL 254
           + EIRRR L++++ GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVEA S  NGIG+
Sbjct: 204 YKEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEATSVENGIGI 263

Query: 255 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKENGHAVLVVAE 314
           VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG GGL+ F+ ++L+ENGH V+V+AE
Sbjct: 264 VKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGKGGLYEFIAKRLRENGHMVIVIAE 323

Query: 315 GAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTVKYIDPTYMI 374
           GAGQD++  S   +E++D SGN +  DVG W+  ++K ++A+ +  ++ T+KYIDPTYMI
Sbjct: 324 GAGQDLVAES---IEQQDASGNKLLKDVGLWMSLKIKEYFAKHNVMDI-TLKYIDPTYMI 383

Query: 375 RAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEAKNEVDARDH 434
           RA+PANA+DN+Y TLLA SA+HG MAGYTGFV G +NG ++YIP   + E +N+V   D 
Sbjct: 384 RAIPANASDNVYSTLLAQSAVHGAMAGYTGFVSGLVNGRHTYIPFNRITERQNKVVITDR 443

Query: 435 KWAWVRSVTNQPDFV 450
            WA + S TNQP F+
Sbjct: 444 MWARMLSSTNQPSFM 451

BLAST of Sed0004264 vs. ExPASy Swiss-Prot
Match: Q9C5J7 (ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE=1 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 7.3e-144
Identity = 250/435 (57.47%), Postives = 318/435 (73.10%), Query Frame = 0

Query: 15  LPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLTAFPGHVAYHRA 74
           +PHL D  P+L   T PNPL  N  +  +  ++V   D V  ++       P  V + RA
Sbjct: 23  VPHLIDYLPDL--PTYPNPLQDNPAYSVVKQYFVHADDSVPEKVVVHKDG-PRGVHFRRA 82

Query: 75  GPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIVAGYRGFYST 134
           GPR+ VYF+ D+V A IVTCGGLCPGLNTVIRE+V  L  +YGV+RI GI  GYRGFY+ 
Sbjct: 83  GPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGGYRGFYAK 142

Query: 135 DSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGDGTMRGAVKI 194
           +++ LN  +V D HKRGG+ + TSRGG D  KIVD++QDRG NQVYIIGGDGT RGA  I
Sbjct: 143 NTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGTQRGASVI 202

Query: 195 FDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIGL 254
           F+EIRRR L++++ GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVEAES  NGIG 
Sbjct: 203 FEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESNENGIGF 262

Query: 255 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKENGHAVLVVAE 314
           VKLMGR +G+IA++ATL+SRDVDCCLIPE  FYLEG+GGLF F++R+LK++GH V+V+AE
Sbjct: 263 VKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGHMVIVLAE 322

Query: 315 GAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTVKYIDPTYMI 374
           GAGQD++ +S  +    D SGN +  DVG WL   +K+ + +     +  +KYIDPTYMI
Sbjct: 323 GAGQDLMCKS-MESTPMDASGNKLLKDVGLWLSQSIKDHFKKNK--MVMNLKYIDPTYMI 382

Query: 375 RAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEAKNEVDARDH 434
           RAVP+NA+DN+YCTLLA SA+HG MAGYTG+  G +NG  +YIP   + E +N V   D 
Sbjct: 383 RAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQNNVVITDR 442

Query: 435 KWAWVRSVTNQPDFV 450
            WA + S TNQP F+
Sbjct: 443 MWARLLSSTNQPSFL 451

BLAST of Sed0004264 vs. ExPASy TrEMBL
Match: A0A6J1ISR0 (ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111478077 PE=3 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 7.6e-229
Identity = 388/458 (84.72%), Postives = 421/458 (91.92%), Query Frame = 0

Query: 1   MAAAAAAAEIS-------VAKLPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDV 60
           MA A AAAEIS       VA LPHLTD FP L++RT  NPL +NQFFHP+DGFY   TDV
Sbjct: 1   MATATAAAEISDRHPPIAVASLPHLTDHFPTLQSRT--NPLQNNQFFHPIDGFYATSTDV 60

Query: 61  VLRQIAFDLT--AFPGHVAYHRAGPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVG 120
           VLR+IAFDL+    P HVAY+RAGPRRSVYF+P+ VRAAIVTCGGLCPG+NTVIRELVVG
Sbjct: 61  VLRRIAFDLSRKPSPAHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVG 120

Query: 121 LWDLYGVRRIDGIVAGYRGFYSTDSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDAL 180
           LW+LYGVR+I GIVAGYRGFYS++ VEL+  +V DWHKRGG+AL TSRGGFDLR+IVDA+
Sbjct: 121 LWELYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAI 180

Query: 181 QDRGFNQVYIIGGDGTMRGAVKIFDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAV 240
           QDRGFNQVYIIGGDGTMRGAVKIF+E+ RR LQ+++TGIPKTVDNDIGIIDRSFGFQTAV
Sbjct: 181 QDRGFNQVYIIGGDGTMRGAVKIFEEVCRRELQVAVTGIPKTVDNDIGIIDRSFGFQTAV 240

Query: 241 EMAQQAINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGD 300
           EMAQQAI+AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGD
Sbjct: 241 EMAQQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGD 300

Query: 301 GGLFPFLDRKLKENGHAVLVVAEGAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELK 360
           GGLFPFL+R+LKENGHAVLVVAEGAGQD IPR+EAQ EERDESGNPVFLDVGGWL+TELK
Sbjct: 301 GGLFPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELK 360

Query: 361 NWWARTHPGELFTVKYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPIN 420
           +WWARTHPGELFTVKYIDPTYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPIN
Sbjct: 361 DWWARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPIN 420

Query: 421 GNYSYIPLKEVAEAKNEVDARDHKWAWVRSVTNQPDFV 450
           GNYSYIPLK+VAEAKNEV+ +DHKWAWVRSVTNQPDFV
Sbjct: 421 GNYSYIPLKDVAEAKNEVNTKDHKWAWVRSVTNQPDFV 456

BLAST of Sed0004264 vs. ExPASy TrEMBL
Match: A0A6J1GG28 (ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC111453612 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 1.0e-228
Identity = 387/455 (85.05%), Postives = 420/455 (92.31%), Query Frame = 0

Query: 4   AAAAAE-------ISVAKLPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLR 63
           A AAAE       I+VA LPHLTD FP L++RT  NPL +NQFFHP+DGFY   TDVVLR
Sbjct: 2   ATAAAEIADRHPPIAVASLPHLTDHFPTLQSRT--NPLQNNQFFHPIDGFYATSTDVVLR 61

Query: 64  QIAFDLT--AFPGHVAYHRAGPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWD 123
           +IAFDL+    P HVAY+RAGPRRSVYF+P+ VRAAIVTCGGLCPG+NTVIRELVVGLW+
Sbjct: 62  RIAFDLSRKPSPAHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWE 121

Query: 124 LYGVRRIDGIVAGYRGFYSTDSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDR 183
           LYGVR+I GIVAGYRGFYS++ VEL+  +V DWHKRGG+AL TSRGGFDLR+IVDA+QDR
Sbjct: 122 LYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDR 181

Query: 184 GFNQVYIIGGDGTMRGAVKIFDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMA 243
           GFNQVYIIGGDGTMRGAVKIF+E+ RRNLQ+++TGIPKTVDNDIGIIDRSFGFQTAVEMA
Sbjct: 182 GFNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMA 241

Query: 244 QQAINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGL 303
           QQAI+AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGL
Sbjct: 242 QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGL 301

Query: 304 FPFLDRKLKENGHAVLVVAEGAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWW 363
           FPFL+R+LKENGHAVLVVAEGAGQD IPR+EAQ EERDESGNPVFLDVGGWL+TELK+WW
Sbjct: 302 FPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWW 361

Query: 364 ARTHPGELFTVKYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNY 423
           ARTHPGELFTVKYIDPTYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPINGNY
Sbjct: 362 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNY 421

Query: 424 SYIPLKEVAEAKNEVDARDHKWAWVRSVTNQPDFV 450
           SYIPLK+VAEAKNEV+ RDHKWAWVRSVTNQPDFV
Sbjct: 422 SYIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFV 454

BLAST of Sed0004264 vs. ExPASy TrEMBL
Match: A0A0A0KL11 (ATP-dependent 6-phosphofructokinase OS=Cucumis sativus OX=3659 GN=PFK PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 8.4e-228
Identity = 379/442 (85.75%), Postives = 411/442 (92.99%), Query Frame = 0

Query: 10  ISVAKLPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLT--AFPG 69
           I+VA LPHLTD FP L+TRT  NPL +NQFFHP+DGFY   TD+VLR+IAFDL+    P 
Sbjct: 13  ITVADLPHLTDNFPNLKTRT--NPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGDTSPA 72

Query: 70  HVAYHRAGPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIVAG 129
           HVAY RAGPRR VYF+P+ VRAAIVTCGGLCPG+NTVIRELVVGLW+LYGVR+I GIVAG
Sbjct: 73  HVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIYGIVAG 132

Query: 130 YRGFYSTDSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGDGT 189
           YRGFYST+ VELN  +V +WHKRGG+AL+TSRGGFDL+KIVDA+++ GFNQVYI GGDGT
Sbjct: 133 YRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQVYITGGDGT 192

Query: 190 MRGAVKIFDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAES 249
           MRGAVKIF+E+RRRNLQ+++TGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAES
Sbjct: 193 MRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAES 252

Query: 250 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKENGH 309
           AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG+GGLFPFL+R+LKENGH
Sbjct: 253 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFLERRLKENGH 312

Query: 310 AVLVVAEGAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTVKY 369
           AVLVVAEGAGQ MIPR+EAQ EERDESGNPVFLDVGGWL+TELKNWWA THPGELFTVKY
Sbjct: 313 AVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTHPGELFTVKY 372

Query: 370 IDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEAKN 429
           IDPTYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPINGNYSYIPLKEVAEAKN
Sbjct: 373 IDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKN 432

Query: 430 EVDARDHKWAWVRSVTNQPDFV 450
           EV+ RDHKWAWVRSVTNQPDFV
Sbjct: 433 EVNTRDHKWAWVRSVTNQPDFV 452

BLAST of Sed0004264 vs. ExPASy TrEMBL
Match: A0A1S3B325 (ATP-dependent 6-phosphofructokinase OS=Cucumis melo OX=3656 GN=LOC103485214 PE=3 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.4e-227
Identity = 380/442 (85.97%), Postives = 410/442 (92.76%), Query Frame = 0

Query: 10  ISVAKLPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLT--AFPG 69
           I+VA LPHLTD FP L TRT  NPL +NQFFHP+DGFY   TD+VLR+IAFDL+    P 
Sbjct: 13  IAVADLPHLTDNFPNLETRT--NPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGNTSPA 72

Query: 70  HVAYHRAGPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIVAG 129
           HVAY RAGPRR+VYF+P+ VRAAIVTCGGLCPG+NTVIRELVVGLW+LYGVRRI GIVAG
Sbjct: 73  HVAYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRRIYGIVAG 132

Query: 130 YRGFYSTDSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGDGT 189
           YRGFYS + VELN  +V +WHKRGG+AL TSRGGFDL+KIVDA+++ GFNQVYI GGDGT
Sbjct: 133 YRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGFNQVYITGGDGT 192

Query: 190 MRGAVKIFDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAES 249
           MRGAVKIF+EIRRRNLQ+++TGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAES
Sbjct: 193 MRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAES 252

Query: 250 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKENGH 309
           AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEG+GGLFPFL+++LKENGH
Sbjct: 253 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFPFLEQRLKENGH 312

Query: 310 AVLVVAEGAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTVKY 369
           AVLVVAEGAGQ MIPR+EAQ EERDESGNPVFLDVGGWL+TELKNWW RTHPGELFTVKY
Sbjct: 313 AVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWERTHPGELFTVKY 372

Query: 370 IDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEAKN 429
           IDPTYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPINGNYSYIPLKEVAEAKN
Sbjct: 373 IDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKN 432

Query: 430 EVDARDHKWAWVRSVTNQPDFV 450
           EV+ RDHKWAWVRSVTNQPDFV
Sbjct: 433 EVNTRDHKWAWVRSVTNQPDFV 452

BLAST of Sed0004264 vs. ExPASy TrEMBL
Match: A0A5D3CPV9 (ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PFK PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 5.5e-227
Identity = 379/442 (85.75%), Postives = 409/442 (92.53%), Query Frame = 0

Query: 10  ISVAKLPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLT--AFPG 69
           I+VA LPHLTD FP L TRT  NPL +NQFFHP+DGFY   TD+VLR+IAFDL+    P 
Sbjct: 13  IAVADLPHLTDNFPNLETRT--NPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGNTSPA 72

Query: 70  HVAYHRAGPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIVAG 129
           HVAY RAGPRR+VYF+P+ VRAAIVTCGGLCPG+NTVIRELVVGLW+LYGVRRI GIVAG
Sbjct: 73  HVAYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRRIYGIVAG 132

Query: 130 YRGFYSTDSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGDGT 189
           YRGFYS + VELN  +V +WHKRGG+AL TSRGGFDL+KIVDA+++ GFNQVYI GGDGT
Sbjct: 133 YRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGFNQVYITGGDGT 192

Query: 190 MRGAVKIFDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAES 249
           MRGAVKIF+EIRRRNLQ+++TGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAES
Sbjct: 193 MRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAES 252

Query: 250 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKENGH 309
           AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEG+GGLFPFL+++LKENGH
Sbjct: 253 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFPFLEQRLKENGH 312

Query: 310 AVLVVAEGAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTVKY 369
           AVLVVAEGAGQ MIPR+EAQ EERDESGNPVFLDVGGWL+TELKNWW RTHPGELFTVKY
Sbjct: 313 AVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWERTHPGELFTVKY 372

Query: 370 IDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEAKN 429
           IDPTYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPINGNYSYIPLKEVAEAKN
Sbjct: 373 IDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKN 432

Query: 430 EVDARDHKWAWVRSVTNQPDFV 450
           EV+ RDHKWAWVRSV NQPDFV
Sbjct: 433 EVNTRDHKWAWVRSVANQPDFV 452

BLAST of Sed0004264 vs. TAIR 10
Match: AT5G47810.1 (phosphofructokinase 2 )

HSP 1 Score: 705.3 bits (1819), Expect = 3.2e-203
Identity = 335/443 (75.62%), Postives = 380/443 (85.78%), Query Frame = 0

Query: 7   AAEISVAKLPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLTAF- 66
           AAE S+ KLP L+        R R NPL  N +FHP +GFY+ P+DV+L Q+A+D +A  
Sbjct: 2   AAETSIRKLPSLSG------LRHRRNPLEDNPYFHPSNGFYITPSDVILAQVAYDHSAHS 61

Query: 67  PGHVAYHRAGPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIV 126
              VAYHRAGPRR + ++P  V+AAIVTCGGLCPG+NTVIRELVVGLW+LYGVR I GI 
Sbjct: 62  QSRVAYHRAGPRREIMYEPSAVKAAIVTCGGLCPGMNTVIRELVVGLWELYGVREIYGIP 121

Query: 127 AGYRGFYSTDSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGD 186
           AGYRGFYS  +V+L+P  V DWHK+GG+ L TSRGGF L+KIVDA+   G+NQVYIIGGD
Sbjct: 122 AGYRGFYSMKAVKLDPKAVHDWHKKGGTVLATSRGGFHLQKIVDAIHLNGYNQVYIIGGD 181

Query: 187 GTMRGAVKIFDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEA 246
           GTMRGAV+IF EI  R L++ IT IPKTVDND+GIIDRSFGFQTAVEMAQ+AI+AAHVEA
Sbjct: 182 GTMRGAVEIFKEISLRKLEVGITVIPKTVDNDVGIIDRSFGFQTAVEMAQEAISAAHVEA 241

Query: 247 ESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKEN 306
           ESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM+FYLEG GGLF FL+++LKE 
Sbjct: 242 ESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMDFYLEGKGGLFEFLEKRLKER 301

Query: 307 GHAVLVVAEGAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTV 366
           GHAVLVVAEGAGQ+MIPR+E+Q +ERDESGN VFLDVG W ++ LK WW R HP ELFTV
Sbjct: 302 GHAVLVVAEGAGQEMIPRNESQKQERDESGNAVFLDVGVWFKSVLKAWWEREHPDELFTV 361

Query: 367 KYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEA 426
           KYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNY+YIPL+EVA+ 
Sbjct: 362 KYIDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYAYIPLEEVAQT 421

Query: 427 KNEVDARDHKWAWVRSVTNQPDF 449
           KN+V+ RDHKWAWVRSVTNQPDF
Sbjct: 422 KNQVNTRDHKWAWVRSVTNQPDF 438

BLAST of Sed0004264 vs. TAIR 10
Match: AT4G26270.1 (phosphofructokinase 3 )

HSP 1 Score: 516.5 bits (1329), Expect = 2.1e-146
Identity = 252/435 (57.93%), Postives = 320/435 (73.56%), Query Frame = 0

Query: 15  LPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLTAFPGHVAYHRA 74
           +PHL+D  P L   T PNPL  N  +  +  ++V   D V ++I       P  + + RA
Sbjct: 23  VPHLSDYLPGL--PTYPNPLQDNPAYSVVKQYFVDADDSVPQKIVVHKDG-PRGIHFRRA 82

Query: 75  GPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIVAGYRGFYST 134
           GPR+ VYF+ D+V A IVTCGGLCPGLNTVIRE+V  L  +YGV+RI GI  GYRGFY+ 
Sbjct: 83  GPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGGYRGFYAK 142

Query: 135 DSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGDGTMRGAVKI 194
           ++V L+  +V D HKRGG+ L TSRGG D  KIVD++QDRG NQVYIIGGDGT RGA  I
Sbjct: 143 NTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGINQVYIIGGDGTQRGASVI 202

Query: 195 FDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIGL 254
           F+EIRRR L++++ GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVEAES  NGIG+
Sbjct: 203 FEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESIENGIGV 262

Query: 255 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKENGHAVLVVAE 314
           VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG+GGLF +++++LKE+GH VLV+AE
Sbjct: 263 VKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGHMVLVIAE 322

Query: 315 GAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTVKYIDPTYMI 374
           GAGQD++ +S   M  +D SGN +  DVG WL   +K+ +       +  +KYIDPTYMI
Sbjct: 323 GAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDHF--NQKKMVMNLKYIDPTYMI 382

Query: 375 RAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEAKNEVDARDH 434
           RAVP+NA+DN+YCTLLA SA+HG MAGYTG++ G +NG  +YIP   + E +N V   D 
Sbjct: 383 RAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTYIPFYRITEKQNHVVITDR 442

Query: 435 KWAWVRSVTNQPDFV 450
            WA + S TNQP F+
Sbjct: 443 MWARLLSSTNQPSFL 452

BLAST of Sed0004264 vs. TAIR 10
Match: AT5G61580.1 (phosphofructokinase 4 )

HSP 1 Score: 514.6 bits (1324), Expect = 8.0e-146
Identity = 246/435 (56.55%), Postives = 318/435 (73.10%), Query Frame = 0

Query: 15  LPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLTAFPGHVAYHRA 74
           +PHLT   P+L   + PNPL  +Q +  +   +V+  DVV + I     +  G V + RA
Sbjct: 74  VPHLTKFLPDL--PSYPNPLKESQAYAIVKRTFVSSEDVVAQNIVVQKGSKRG-VHFRRA 133

Query: 75  GPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIVAGYRGFYST 134
           GPR  VYF  D+V+A IVTCGGLCPG+NTVIRE+V GL ++YGV  I GI  GYRGFYS 
Sbjct: 134 GPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGGYRGFYSK 193

Query: 135 DSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGDGTMRGAVKI 194
           +++ L P +V D HKRGG+ L+TSRGG D  KIVD +QDRG NQVYIIGG GT +GA KI
Sbjct: 194 NTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGTQKGAEKI 253

Query: 195 FDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIGL 254
           ++E+ RR LQ++++GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVE ES  NG+G+
Sbjct: 254 YEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVESVENGVGI 313

Query: 255 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKENGHAVLVVAE 314
           VKLMGR +G IA+ ATL++RDVDCCLIPE  F+LEG GGLF F++ +LKEN H V+V+AE
Sbjct: 314 VKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRHMVIVIAE 373

Query: 315 GAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTVKYIDPTYMI 374
           GAGQD + +S    E +D SGN + LDVG WL  ++K+ +       +  +KYIDPTYMI
Sbjct: 374 GAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRK-MMINMKYIDPTYMI 433

Query: 375 RAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEAKNEVDARDH 434
           RA+P+NA+DN+YCTLLA SA+HG MAGY+GF  GP+N  ++YIP+ +V E  N V   D 
Sbjct: 434 RAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTNTVKLTDR 493

Query: 435 KWAWVRSVTNQPDFV 450
            WA + + TNQP F+
Sbjct: 494 MWARLLASTNQPSFL 504

BLAST of Sed0004264 vs. TAIR 10
Match: AT4G32840.1 (phosphofructokinase 6 )

HSP 1 Score: 512.7 bits (1319), Expect = 3.0e-145
Identity = 250/435 (57.47%), Postives = 320/435 (73.56%), Query Frame = 0

Query: 15  LPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLTAFPGHVAYHRA 74
           +PHL+D   +L   T PNPL SN  +  +  ++V   D V  +I     + P    + RA
Sbjct: 24  VPHLSDYILDL--PTYPNPLQSNAAYSVVRQYFVDEDDTVQEKIVVHKDS-PRGTHFRRA 83

Query: 75  GPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIVAGYRGFYST 134
           GPR+ VYF P DVRA IVTCGGLCPGLNTVIRE+V GL  +YGV  + G+  G+RGFYS 
Sbjct: 84  GPRQKVYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHFMYGVTEVIGVDCGFRGFYSK 143

Query: 135 DSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGDGTMRGAVKI 194
           ++V L P  V D HKRGG+ L TSRGG D  KIVD +QDR  NQVYIIGGDGT +GA  I
Sbjct: 144 NTVALTPKTVSDIHKRGGTILGTSRGGHDTSKIVDNIQDREINQVYIIGGDGTQKGANAI 203

Query: 195 FDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIGL 254
           + EIRRR L++++ GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVEA S  NGIG+
Sbjct: 204 YKEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEATSVENGIGI 263

Query: 255 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKENGHAVLVVAE 314
           VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG GGL+ F+ ++L+ENGH V+V+AE
Sbjct: 264 VKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGKGGLYEFIAKRLRENGHMVIVIAE 323

Query: 315 GAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTVKYIDPTYMI 374
           GAGQD++  S   +E++D SGN +  DVG W+  ++K ++A+ +  ++ T+KYIDPTYMI
Sbjct: 324 GAGQDLVAES---IEQQDASGNKLLKDVGLWMSLKIKEYFAKHNVMDI-TLKYIDPTYMI 383

Query: 375 RAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEAKNEVDARDH 434
           RA+PANA+DN+Y TLLA SA+HG MAGYTGFV G +NG ++YIP   + E +N+V   D 
Sbjct: 384 RAIPANASDNVYSTLLAQSAVHGAMAGYTGFVSGLVNGRHTYIPFNRITERQNKVVITDR 443

Query: 435 KWAWVRSVTNQPDFV 450
            WA + S TNQP F+
Sbjct: 444 MWARMLSSTNQPSFM 451

BLAST of Sed0004264 vs. TAIR 10
Match: AT5G56630.1 (phosphofructokinase 7 )

HSP 1 Score: 511.9 bits (1317), Expect = 5.2e-145
Identity = 250/435 (57.47%), Postives = 318/435 (73.10%), Query Frame = 0

Query: 15  LPHLTDTFPELRTRTRPNPLHSNQFFHPMDGFYVAPTDVVLRQIAFDLTAFPGHVAYHRA 74
           +PHL D  P+L   T PNPL  N  +  +  ++V   D V  ++       P  V + RA
Sbjct: 23  VPHLIDYLPDL--PTYPNPLQDNPAYSVVKQYFVHADDSVPEKVVVHKDG-PRGVHFRRA 82

Query: 75  GPRRSVYFDPDDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRRIDGIVAGYRGFYST 134
           GPR+ VYF+ D+V A IVTCGGLCPGLNTVIRE+V  L  +YGV+RI GI  GYRGFY+ 
Sbjct: 83  GPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGGYRGFYAK 142

Query: 135 DSVELNPVMVRDWHKRGGSALETSRGGFDLRKIVDALQDRGFNQVYIIGGDGTMRGAVKI 194
           +++ LN  +V D HKRGG+ + TSRGG D  KIVD++QDRG NQVYIIGGDGT RGA  I
Sbjct: 143 NTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGTQRGASVI 202

Query: 195 FDEIRRRNLQISITGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIGL 254
           F+EIRRR L++++ GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVEAES  NGIG 
Sbjct: 203 FEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESNENGIGF 262

Query: 255 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLDRKLKENGHAVLVVAE 314
           VKLMGR +G+IA++ATL+SRDVDCCLIPE  FYLEG+GGLF F++R+LK++GH V+V+AE
Sbjct: 263 VKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGHMVIVLAE 322

Query: 315 GAGQDMIPRSEAQMEERDESGNPVFLDVGGWLRTELKNWWARTHPGELFTVKYIDPTYMI 374
           GAGQD++ +S  +    D SGN +  DVG WL   +K+ + +     +  +KYIDPTYMI
Sbjct: 323 GAGQDLMCKS-MESTPMDASGNKLLKDVGLWLSQSIKDHFKKNK--MVMNLKYIDPTYMI 382

Query: 375 RAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYSYIPLKEVAEAKNEVDARDH 434
           RAVP+NA+DN+YCTLLA SA+HG MAGYTG+  G +NG  +YIP   + E +N V   D 
Sbjct: 383 RAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQNNVVITDR 442

Query: 435 KWAWVRSVTNQPDFV 450
            WA + S TNQP F+
Sbjct: 443 MWARLLSSTNQPSFL 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038881531.12.2e-23087.10ATP-dependent 6-phosphofructokinase 2 isoform X1 [Benincasa hispida][more]
XP_023544772.12.4e-22985.09ATP-dependent 6-phosphofructokinase 2 [Cucurbita pepo subsp. pepo][more]
KAG6603845.15.4e-22985.09ATP-dependent 6-phosphofructokinase 2, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022977934.11.6e-22884.72ATP-dependent 6-phosphofructokinase 2 [Cucurbita maxima][more]
XP_022950529.12.1e-22885.05ATP-dependent 6-phosphofructokinase 2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FIK04.5e-20275.62ATP-dependent 6-phosphofructokinase 2 OS=Arabidopsis thaliana OX=3702 GN=PFK2 PE... [more]
Q94AA43.0e-14557.93ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE... [more]
Q9FKG31.1e-14456.55ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q9M0764.3e-14457.47ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE... [more]
Q9C5J77.3e-14457.47ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1ISR07.6e-22984.72ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111478077 ... [more]
A0A6J1GG281.0e-22885.05ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC11145361... [more]
A0A0A0KL118.4e-22885.75ATP-dependent 6-phosphofructokinase OS=Cucumis sativus OX=3659 GN=PFK PE=3 SV=1[more]
A0A1S3B3251.4e-22785.97ATP-dependent 6-phosphofructokinase OS=Cucumis melo OX=3656 GN=LOC103485214 PE=3... [more]
A0A5D3CPV95.5e-22785.75ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PF... [more]
Match NameE-valueIdentityDescription
AT5G47810.13.2e-20375.62phosphofructokinase 2 [more]
AT4G26270.12.1e-14657.93phosphofructokinase 3 [more]
AT5G61580.18.0e-14656.55phosphofructokinase 4 [more]
AT4G32840.13.0e-14557.47phosphofructokinase 6 [more]
AT5G56630.15.2e-14557.47phosphofructokinase 7 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022953ATP-dependent 6-phosphofructokinasePRINTSPR00476PHFRCTKINASEcoord: 226..244
score: 26.32
coord: 208..225
score: 36.18
coord: 304..316
score: 37.28
coord: 175..191
score: 43.89
coord: 91..110
score: 41.92
coord: 247..263
score: 28.51
IPR012004Pyrophosphate-dependent phosphofructokinase TP0108-typePIRSFPIRSF000534ATP_PFK_TP0108coord: 12..451
e-value: 1.6E-172
score: 572.0
IPR012004Pyrophosphate-dependent phosphofructokinase TP0108-typeHAMAPMF_01981Phosphofructokinase_II_Xcoord: 15..449
score: 52.128403
IPR000023Phosphofructokinase domainPFAMPF00365PFKcoord: 89..396
e-value: 3.6E-54
score: 184.0
NoneNo IPR availableGENE3D3.40.50.450coord: 83..272
e-value: 1.1E-55
score: 189.9
NoneNo IPR availablePANTHERPTHR45770:SF9ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 2coord: 5..449
NoneNo IPR availablePANTHERPTHR45770FAMILY NOT NAMEDcoord: 5..449
IPR035966Phosphofructokinase superfamilySUPERFAMILY53784Phosphofructokinasecoord: 30..449

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0004264.1Sed0004264.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006002 fructose 6-phosphate metabolic process
biological_process GO:0061615 glycolytic process through fructose-6-phosphate
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003872 6-phosphofructokinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0046872 metal ion binding