Homology
BLAST of Sed0003769 vs. NCBI nr
Match:
XP_022152631.1 (DNA topoisomerase 2 isoform X1 [Momordica charantia])
HSP 1 Score: 944.1 bits (2439), Expect = 5.4e-271
Identity = 480/643 (74.65%), Postives = 509/643 (79.16%), Query Frame = 0
Query: 1 MPAEKKRPMQNSAAANGDPIPGGSNKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLW 60
M EKKRP+QNS AN D P G KTIEEMYQKKTQ+EHILLRPDTYVGS+E HTQ LW
Sbjct: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENGKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGD 120
VYEN KMVHR VTYVPGLYKIF+EILVNAADNKQRDPSMDSVKV IDVE N ISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
Query: 121 GVPVEVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETAD 180
GVPVE+H EEKVY KKTTGGRNG GAKLTNIFST+F IETAD
Sbjct: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRLKKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
GKR KKYKQVF DNMG KSEP+ITKCK +NWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VVDLAGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPPKRYHVKPNERWEIVV 300
V+D+AGCLGKTVKVELNGQR+PV+SFQDYVDLYL S STF TEPPKRYH+KPNERWEIVV
Sbjct: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
Query: 301 ALSEGQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFWI 360
LSEGQF QV+FVN IATIKGGTHVDYITNQIANYV+NVVNKKNKNANVKAHNV SY W+
Sbjct: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FVNALIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKN--------------------- 420
F+NALIDNPAFDSQTKETLTIR S+FGSGC+LTPEFLKN
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
Query: 421 -----DG----------------------RTRCS--------------------GRDFYS 480
DG +C+ GRDFY
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHD 540
VFPLRG +LNVR SS++I +NKEIQ IKQILGLQ GKEYN+ K LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKENLR 557
GSHIKGLLINFIH FWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKE+LR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
BLAST of Sed0003769 vs. NCBI nr
Match:
XP_022152632.1 (DNA topoisomerase 2 isoform X2 [Momordica charantia])
HSP 1 Score: 944.1 bits (2439), Expect = 5.4e-271
Identity = 480/643 (74.65%), Postives = 509/643 (79.16%), Query Frame = 0
Query: 1 MPAEKKRPMQNSAAANGDPIPGGSNKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLW 60
M EKKRP+QNS AN D P G KTIEEMYQKKTQ+EHILLRPDTYVGS+E HTQ LW
Sbjct: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENGKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGD 120
VYEN KMVHR VTYVPGLYKIF+EILVNAADNKQRDPSMDSVKV IDVE N ISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
Query: 121 GVPVEVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETAD 180
GVPVE+H EEKVY KKTTGGRNG GAKLTNIFST+F IETAD
Sbjct: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRLKKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
GKR KKYKQVF DNMG KSEP+ITKCK +NWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VVDLAGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPPKRYHVKPNERWEIVV 300
V+D+AGCLGKTVKVELNGQR+PV+SFQDYVDLYL S STF TEPPKRYH+KPNERWEIVV
Sbjct: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
Query: 301 ALSEGQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFWI 360
LSEGQF QV+FVN IATIKGGTHVDYITNQIANYV+NVVNKKNKNANVKAHNV SY W+
Sbjct: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FVNALIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKN--------------------- 420
F+NALIDNPAFDSQTKETLTIR S+FGSGC+LTPEFLKN
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
Query: 421 -----DG----------------------RTRCS--------------------GRDFYS 480
DG +C+ GRDFY
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHD 540
VFPLRG +LNVR SS++I +NKEIQ IKQILGLQ GKEYN+ K LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKENLR 557
GSHIKGLLINFIH FWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKE+LR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
BLAST of Sed0003769 vs. NCBI nr
Match:
XP_023527826.1 (DNA topoisomerase 2 isoform X6 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 939.9 bits (2428), Expect = 1.0e-269
Identity = 474/643 (73.72%), Postives = 512/643 (79.63%), Query Frame = 0
Query: 1 MPAEKKRPMQNSAAANGDPIPGGSNKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLW 60
M EKKRP+QNS AAN D PGG KTIEEMYQKKTQ+EHILLRPDTYVGS+E HTQ LW
Sbjct: 1 MAVEKKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENGKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGD 120
+YEN KMVHR VTYVPGLYKIF+EILVNAADNKQRDPSMDS+KV ID E N+ISVFNNGD
Sbjct: 61 IYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120
Query: 121 GVPVEVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETAD 180
GVPVEVH EEKVY KKTTGGRNG GAKLTNIFST+F IETAD
Sbjct: 121 GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRLKKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
G+RLKKYKQVF+DNMG KSEPIITKCK +NWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VVDLAGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPPKRYHVKPNERWEIVV 300
V+DLAGCLGKTVKVELNGQR+PV+SFQDYVD YL S + F TEPPKRYH++PNERWEIVV
Sbjct: 241 VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300
Query: 301 ALSEGQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFWI 360
LSEGQF QV+FVNGIATIKGGTHVDYITNQIANYV+ VVNKKNKNANVKAHNV SY W+
Sbjct: 301 TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FVNALIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKN--------------------- 420
F+NALIDNPAFDSQTKETLTIR S+FGS C+LTP+FLKN
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420
Query: 421 -----DG----------------------RTRCS--------------------GRDFYS 480
DG +C+ GRDFY
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHD 540
VFPLRG +LNVR SS++I +NKEIQ IKQILGLQ GKEYN+AK+LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKENLR 557
GSHIKGLLINFIH FWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK+NLR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600
BLAST of Sed0003769 vs. NCBI nr
Match:
XP_023527825.1 (DNA topoisomerase 2 isoform X5 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 939.9 bits (2428), Expect = 1.0e-269
Identity = 474/643 (73.72%), Postives = 512/643 (79.63%), Query Frame = 0
Query: 1 MPAEKKRPMQNSAAANGDPIPGGSNKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLW 60
M EKKRP+QNS AAN D PGG KTIEEMYQKKTQ+EHILLRPDTYVGS+E HTQ LW
Sbjct: 1 MAVEKKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENGKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGD 120
+YEN KMVHR VTYVPGLYKIF+EILVNAADNKQRDPSMDS+KV ID E N+ISVFNNGD
Sbjct: 61 IYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120
Query: 121 GVPVEVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETAD 180
GVPVEVH EEKVY KKTTGGRNG GAKLTNIFST+F IETAD
Sbjct: 121 GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRLKKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
G+RLKKYKQVF+DNMG KSEPIITKCK +NWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VVDLAGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPPKRYHVKPNERWEIVV 300
V+DLAGCLGKTVKVELNGQR+PV+SFQDYVD YL S + F TEPPKRYH++PNERWEIVV
Sbjct: 241 VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300
Query: 301 ALSEGQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFWI 360
LSEGQF QV+FVNGIATIKGGTHVDYITNQIANYV+ VVNKKNKNANVKAHNV SY W+
Sbjct: 301 TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FVNALIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKN--------------------- 420
F+NALIDNPAFDSQTKETLTIR S+FGS C+LTP+FLKN
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420
Query: 421 -----DG----------------------RTRCS--------------------GRDFYS 480
DG +C+ GRDFY
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHD 540
VFPLRG +LNVR SS++I +NKEIQ IKQILGLQ GKEYN+AK+LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKENLR 557
GSHIKGLLINFIH FWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK+NLR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600
BLAST of Sed0003769 vs. NCBI nr
Match:
XP_023527823.1 (DNA topoisomerase 2 isoform X3 [Cucurbita pepo subsp. pepo] >XP_023527824.1 DNA topoisomerase 2 isoform X4 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 939.9 bits (2428), Expect = 1.0e-269
Identity = 474/643 (73.72%), Postives = 512/643 (79.63%), Query Frame = 0
Query: 1 MPAEKKRPMQNSAAANGDPIPGGSNKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLW 60
M EKKRP+QNS AAN D PGG KTIEEMYQKKTQ+EHILLRPDTYVGS+E HTQ LW
Sbjct: 1 MAVEKKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENGKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGD 120
+YEN KMVHR VTYVPGLYKIF+EILVNAADNKQRDPSMDS+KV ID E N+ISVFNNGD
Sbjct: 61 IYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120
Query: 121 GVPVEVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETAD 180
GVPVEVH EEKVY KKTTGGRNG GAKLTNIFST+F IETAD
Sbjct: 121 GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRLKKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
G+RLKKYKQVF+DNMG KSEPIITKCK +NWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VVDLAGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPPKRYHVKPNERWEIVV 300
V+DLAGCLGKTVKVELNGQR+PV+SFQDYVD YL S + F TEPPKRYH++PNERWEIVV
Sbjct: 241 VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300
Query: 301 ALSEGQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFWI 360
LSEGQF QV+FVNGIATIKGGTHVDYITNQIANYV+ VVNKKNKNANVKAHNV SY W+
Sbjct: 301 TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FVNALIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKN--------------------- 420
F+NALIDNPAFDSQTKETLTIR S+FGS C+LTP+FLKN
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420
Query: 421 -----DG----------------------RTRCS--------------------GRDFYS 480
DG +C+ GRDFY
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHD 540
VFPLRG +LNVR SS++I +NKEIQ IKQILGLQ GKEYN+AK+LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKENLR 557
GSHIKGLLINFIH FWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK+NLR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600
BLAST of Sed0003769 vs. ExPASy Swiss-Prot
Match:
O24308 (DNA topoisomerase 2 OS=Pisum sativum OX=3888 GN=TOP2 PE=2 SV=1)
HSP 1 Score: 773.9 bits (1997), Expect = 1.3e-222
Identity = 394/637 (61.85%), Postives = 460/637 (72.21%), Query Frame = 0
Query: 5 KKRPMQNSAAANGDPIPGGSNKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLWVYEN 64
+KRP+Q S AAN IP +KTIEEMYQKKTQ+EHILLRPDTYVGS+E HTQ LWVYEN
Sbjct: 2 EKRPLQTSNAAN---IP---SKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYEN 61
Query: 65 GKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGDGVPV 124
+MVHR V+YVPGLYKIF+EILVNAADNKQRDPSMDS+KV ID E N +SV+NNGDGVPV
Sbjct: 62 DEMVHRAVSYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPV 121
Query: 125 EVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETADGKRL 184
E+H EEKVY KKTTGGRNG GAKLTNIFST+F IETADG+RL
Sbjct: 122 EIHQEEKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETADGRRL 181
Query: 185 KKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKRVVDL 244
KKYKQVF++NMG+K EP+ITKCKA+ENWTKVTFKPDL KF M +LE+DVVALMKKRV+D+
Sbjct: 182 KKYKQVFSNNMGTKCEPVITKCKASENWTKVTFKPDLEKFKMAYLEEDVVALMKKRVLDM 241
Query: 245 AGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPPKRYHVKPNERWEIVVALSE 304
AGC GKTVKVELNG I +SF+DY DL+L P R H K +RWEI ++LS+
Sbjct: 242 AGCFGKTVKVELNGTLIRFKSFRDYADLFLKCAEKSKPMPLPRIHAKVGDRWEICISLSD 301
Query: 305 GQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFWIFVNA 364
GQF QV+FVN IATIKGGTHVDYITNQI Y++N VNKK K+ANVKAH V ++ W+FVN+
Sbjct: 302 GQFQQVSFVNSIATIKGGTHVDYITNQITTYIMNKVNKKKKDANVKAHTVKNHLWVFVNS 361
Query: 365 LIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKN------------------------- 424
LIDNPAFDSQTKETLT R ++FGS CD+ LK+
Sbjct: 362 LIDNPAFDSQTKETLTTRQASFGSKCDVPESMLKDVEKSGIVDTLLSWADFKQSKDLKKT 421
Query: 425 DG---------------------RTRCS--------------------GRDFYSVFPLRG 484
DG +C+ GRD Y VFPLRG
Sbjct: 422 DGTKTQRLRGCKAEDANEAGGRNSEKCTLILTEGDSAKALAMAGLSVVGRDHYGVFPLRG 481
Query: 485 VILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHDGSHIKG 544
+LNVR SS++I N+EIQ IK+ILGLQ KEY + KSLRYGHLMIM DQDHDGSHIKG
Sbjct: 482 KLLNVREASSKQIMDNEEIQNIKKILGLQQNKEYTNVKSLRYGHLMIMADQDHDGSHIKG 541
Query: 545 LLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKENLRGNSSGW 557
LLINFIH FWPSLL+VPSFLVEF TP+++A+H N T+ SFY+MPEYE+WKE L +++ W
Sbjct: 542 LLINFIHSFWPSLLKVPSFLVEFTTPVIRASHPNKTITSFYSMPEYEAWKERLGNSATSW 601
BLAST of Sed0003769 vs. ExPASy Swiss-Prot
Match:
P30182 (DNA topoisomerase 2 OS=Arabidopsis thaliana OX=3702 GN=TOP2 PE=2 SV=2)
HSP 1 Score: 768.8 bits (1984), Expect = 4.1e-221
Identity = 401/644 (62.27%), Postives = 466/644 (72.36%), Query Frame = 0
Query: 6 KRPMQNSAAANGDPIPGGS-----NKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLW 65
K P+QNS AAN P S KTIEEMYQKK+Q+EHILLRPDTY+GS+E HTQTLW
Sbjct: 4 KLPLQNSNAANVAKAPAKSRAAAGGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLW 63
Query: 66 VYENGKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGD 125
VYE +MV R VTYVPGLYKIF+EILVNAADNKQRD MDSV+V IDVE N ISV N+G
Sbjct: 64 VYEKDEMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGA 123
Query: 126 GVPVEVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETAD 185
GVPVE+H EE +Y KKTTGGRNG GAKLTNIFST+F IETAD
Sbjct: 124 GVPVEIHQEEGIYVPEMIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETAD 183
Query: 186 GKRLKKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKR 245
GKRLKKYKQVF +NMG KSEP+ITKC +ENWTKVTFKPDL KFNMT LEDDVVALM KR
Sbjct: 184 GKRLKKYKQVFENNMGKKSEPVITKCNKSENWTKVTFKPDLKKFNMTELEDDVVALMSKR 243
Query: 246 VVDLAGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPP-KRYHVKPNERWEIV 305
V D+AGCLGK+VKVELNG++IPV+SF DYVDLYL++ + TE P R K N+RWE+
Sbjct: 244 VFDIAGCLGKSVKVELNGKQIPVKSFTDYVDLYLSAANKSRTEDPLPRLTEKVNDRWEVC 303
Query: 306 VALSEGQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFW 365
V+LSEGQF QV+FVN IATIKGGTHVDY+T+QI N++V VNKKNKNANVKAHNV ++ W
Sbjct: 304 VSLSEGQFQQVSFVNSIATIKGGTHVDYVTSQITNHIVAAVNKKNKNANVKAHNVKNHLW 363
Query: 366 IFVNALIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKNDGRT---------------- 425
+FVNALIDNPAFDSQTKETLT+R S+FGS C+L+ +FLK G++
Sbjct: 364 VFVNALIDNPAFDSQTKETLTLRQSSFGSKCELSEDFLKKVGKSGVVENLLSWADFKQNK 423
Query: 426 ------------------------------RCS--------------------GRDFYSV 485
C+ G ++ V
Sbjct: 424 DLKKSDGAKTGRVLVEKLDDAAEAGGKNSRLCTLILTEGDSAKSLALAGRSVLGNNYCGV 483
Query: 486 FPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHDG 545
FPLRG +LNVR S+ +I NKEI+ +K+ILGL+ +Y + SLRYG +MIMTDQDHDG
Sbjct: 484 FPLRGKLLNVREASTTQITNNKEIENLKKILGLKQNMKYENVNSLRYGQMMIMTDQDHDG 543
Query: 546 SHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGT--VLSFYTMPEYESWKENL 557
SHIKGLLINFIH FWPSLL+VPSFLVEFITPIVKAT R GT VLSFY+MPEYE WKE+L
Sbjct: 544 SHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKAT-RKGTKKVLSFYSMPEYEEWKESL 603
BLAST of Sed0003769 vs. ExPASy Swiss-Prot
Match:
P08096 (DNA topoisomerase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top2 PE=1 SV=2)
HSP 1 Score: 575.5 bits (1482), Expect = 6.6e-163
Identity = 299/623 (47.99%), Postives = 396/623 (63.56%), Query Frame = 0
Query: 22 GGSNKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLWVY--ENGKMVHRCVTYVPGLY 81
G N + YQ+ T EH+L RPDTY+GS+E T +WV+ E K+ ++ VTYVPGLY
Sbjct: 68 GNGNSNVSTQYQRLTPREHVLRRPDTYIGSIEPTTSEMWVFDSEKNKLDYKAVTYVPGLY 127
Query: 82 KIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGDGVPVEVHLEEKVY------ 141
KIF+EI+VNAADNK RDP+M+++KV +D E N IS++NNG G+P+E+H +EK+Y
Sbjct: 128 KIFDEIIVNAADNKVRDPNMNTLKVTLDPEANVISIYNNGKGIPIEIHDKEKIYIPELIF 187
Query: 142 -------------KKTTGGRNGLGAKLTNIFSTQFFIETADGKRLKKYKQVFNDNMGSKS 201
KK TGGRNG GAKL NIFST+F +ETAD +R+KKYKQ + DNM KS
Sbjct: 188 GNLLTSSNYDDNQKKVTGGRNGYGAKLCNIFSTEFVVETADKERMKKYKQTWYDNMSRKS 247
Query: 202 EPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKRVVDLAGCLGKTVKVELNGQ 261
EP+IT K + +TK+TFKPDLAKF M ++DD+V+++K+R+ D+AG + +T KV LN +
Sbjct: 248 EPVITSLKKPDEYTKITFKPDLAKFGMDKIDDDMVSIIKRRIYDMAGTVRET-KVYLNNE 307
Query: 262 RIPVRSFQDYVDLYLNSVSTFPTEPPKRYHVKPNERWEIVVALSEGQFHQVTFVNGIATI 321
RI + F+ YV++YL S + EPP+ + N+RW++ A+S+GQF QV+FVN I+TI
Sbjct: 308 RISISGFKKYVEMYLASDTKPDEEPPRVIYEHVNDRWDVAFAVSDGQFKQVSFVNNISTI 367
Query: 322 KGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFWIFVNALIDNPAFDSQTKETL 381
+GGTHV+Y+ N+I + + VV K+NK A VKA + +Y +FVN I+NP+FDSQTKETL
Sbjct: 368 RGGTHVNYVANKIVDAIDEVVKKENKKAPVKAFQIKNYVQVFVNCQIENPSFDSQTKETL 427
Query: 382 TIRHSNFGSGCDLTPEFL-------------------------KNDG--RTRCS------ 441
T + S FGS C L+ +FL K DG R+R +
Sbjct: 428 TTKVSAFGSQCTLSDKFLKAIKKSSVVEEVLKFATAKADQQLSKGDGGLRSRITGLTKLE 487
Query: 442 ----------------------------------GRDFYSVFPLRGVILNVRVKSSEEIA 501
GRD+Y VFPLRG +LNVR S +I
Sbjct: 488 DANKAGTKESHKCVLILTEGDSAKSLAVSGLSVVGRDYYGVFPLRGKLLNVREASHSQIL 547
Query: 502 KNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHDGSHIKGLLINFIHYFWPSLL 557
NKEIQ IK+I+G H K Y D K LRYGHLMIMTDQDHDGSHIKGL+IN++ +PSLL
Sbjct: 548 NNKEIQAIKKIMGFTHKKTYTDVKGLRYGHLMIMTDQDHDGSHIKGLIINYLESSYPSLL 607
BLAST of Sed0003769 vs. ExPASy Swiss-Prot
Match:
Q55BP5 (Probable DNA topoisomerase 2 OS=Dictyostelium discoideum OX=44689 GN=top2 PE=3 SV=1)
HSP 1 Score: 545.0 bits (1403), Expect = 9.6e-154
Identity = 295/621 (47.50%), Postives = 386/621 (62.16%), Query Frame = 0
Query: 26 KTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLWVYENGKMVHRCVTYVPGLYKIFNEI 85
K+IEE+YQKK ++ ILLRPDTY+GS E + LWV+E+ +MVHR V++VPG+YKIF+EI
Sbjct: 101 KSIEEIYQKKELLDQILLRPDTYIGSTERQEEELWVWEDKRMVHRKVSFVPGIYKIFDEI 160
Query: 86 LVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGDGVPVEVHLEEKVY------------ 145
LVNAADNKQR+ +M +KV ID E +ISV N+G G+P++VH E K+Y
Sbjct: 161 LVNAADNKQREGNMKFIKVVIDREKGFISVTNDGAGIPIQVHSEHKIYIPELIFGNLLTS 220
Query: 146 -------KKTTGGRNGLGAKLTNIFSTQFFIETADGKRLKKYKQVFNDNMGSKSEPIITK 205
K+ TGGRNG GAKL NIFST+F +E AD K K YKQ F +NM SK +P IT
Sbjct: 221 SHYDDSEKRLTGGRNGFGAKLANIFSTKFIVECADSKSKKLYKQTFTNNMRSKEDPKITS 280
Query: 206 CKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKRVVDLAGCLGKTVKVELNGQRIPVRS 265
+ ++TK+TF P+L +F M +DD+VAL+ KRV D+AGC T+KV LN + + +RS
Sbjct: 281 YQNKTDYTKITFYPELDRFGMVEFDDDLVALLSKRVYDIAGC-NPTLKVSLNDEELGIRS 340
Query: 266 FQDYVDLYLNSVSTFPTE--PPKRYHVKPNERWEIVVALS-EGQFHQVTFVNGIATIKGG 325
F+ Y++LY FP E PK Y+ K ++RWE+ V LS E QF+QV+FVN I T+KGG
Sbjct: 341 FEKYINLY------FPDEENAPKVYYEKVSDRWELAVTLSKESQFNQVSFVNSICTVKGG 400
Query: 326 THVDYITNQIANYVVNVVNKKNK-NANVKAHNVNSYFWIFVNALIDNPAFDSQTKETLTI 385
THV + + I + VNKKNK +A++K + ++ +FVN LI+NP FDSQTKETLT
Sbjct: 401 THVTHALSNIVTAIQEQVNKKNKGSADIKPAFIKNHLSVFVNCLIENPHFDSQTKETLTS 460
Query: 386 RHSNFGSGCDLTPEFLKN------------------------------------------ 445
+ S+FGS C+ + +F+K
Sbjct: 461 KISSFGSRCEPSEKFIKRVLDSKSGIVASILEFAQFKDQSALKRSTSSGGKKGKVSIPKL 520
Query: 446 -----DGRTR---CS--------------------GRDFYSVFPLRGVILNVRVKSSEEI 505
G T+ C+ G+D Y FPL+G +LNVR +S+ ++
Sbjct: 521 DDANLAGGTKSEDCTLILTEGDSAKSLATAGISVVGKDHYGAFPLKGKLLNVRDQST-KV 580
Query: 506 AKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHDGSHIKGLLINFIHYFWPSL 554
N EI I ILGL++GK Y LRYGHLMIMTDQDHDGSHIKGL+IN +H+FWP+L
Sbjct: 581 INNDEINNIVTILGLKYGKVYETLSDLRYGHLMIMTDQDHDGSHIKGLIINMVHHFWPTL 640
BLAST of Sed0003769 vs. ExPASy Swiss-Prot
Match:
O46374 (DNA topoisomerase 2-alpha OS=Sus scrofa OX=9823 GN=TOP2A PE=2 SV=1)
HSP 1 Score: 532.3 bits (1370), Expect = 6.4e-150
Identity = 304/622 (48.87%), Postives = 384/622 (61.74%), Query Frame = 0
Query: 27 TIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLWVY-ENGKMVHRCVTYVPGLYKIFNEI 86
+IE +YQKKTQ+EHILLRPDTY+GSVE+ TQ +WVY E+ + +R VT+VPGLYKIF+EI
Sbjct: 29 SIERIYQKKTQLEHILLRPDTYIGSVESVTQQMWVYDEDIGINYREVTFVPGLYKIFDEI 88
Query: 87 LVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGDGVPVEVHLEEKVY------------ 146
LVNAADNKQRDP M ++V ID E N IS++NNG G+PV H EK+Y
Sbjct: 89 LVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTS 148
Query: 147 -------KKTTGGRNGLGAKLTNIFSTQFFIETADGKRLKKYKQVFNDNMGSKSEPIITK 206
KK TGGRNG GAKL NIFST+F +ETA + K +KQ + DNMG E +
Sbjct: 149 SNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKP 208
Query: 207 CKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKRVVDLAGCLGKTVKVELNGQRIPVRS 266
E++T +TF PDL+KF M L+ D+VALM +R D+AG K VKV LNG ++PV+
Sbjct: 209 FNG-EDYTCITFHPDLSKFKMQSLDKDIVALMVRRAYDIAGST-KDVKVFLNGNKLPVKG 268
Query: 267 FQDYVDLYLNSVSTFPTEPPKRYHVKPNERWEIVVALSEGQFHQVTFVNGIATIKGGTHV 326
F+ YVDLYL P K H + N RWE+ + +SE F Q++FVN IAT KGG HV
Sbjct: 269 FRSYVDLYLKDKVDETGNPLKIIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHV 328
Query: 327 DYITNQIANYVVNVVNKKNK-NANVKAHNVNSYFWIFVNALIDNPAFDSQTKETLTIRHS 386
DY+ +QI +V+VV KKNK VKAH V ++ WIFVNALI+NP FDSQTKE +T++
Sbjct: 329 DYVADQIVAKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVK 388
Query: 387 NFGSGCDLTPEFLK--------------------------------------------ND 446
+FGS C L+ +F+K ND
Sbjct: 389 SFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDAND 448
Query: 447 --GR--TRCS--------------------GRDFYSVFPLRGVILNVRVKSSEEIAKNKE 506
GR T C+ GRD Y VFPLRG ILNVR S ++I +N E
Sbjct: 449 AGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAE 508
Query: 507 IQEIKQILGLQHGKEYND---AKSLRYGHLMIMTDQDHDGSHIKGLLINFIHYFWPSLLR 557
I I +I+GLQ+ K Y D K+LRYG +MIMTDQD DGSHIKGLLINFIH+ WPSLLR
Sbjct: 509 INNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLR 568
BLAST of Sed0003769 vs. ExPASy TrEMBL
Match:
A0A6J1DGT3 (DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1)
HSP 1 Score: 944.1 bits (2439), Expect = 2.6e-271
Identity = 480/643 (74.65%), Postives = 509/643 (79.16%), Query Frame = 0
Query: 1 MPAEKKRPMQNSAAANGDPIPGGSNKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLW 60
M EKKRP+QNS AN D P G KTIEEMYQKKTQ+EHILLRPDTYVGS+E HTQ LW
Sbjct: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENGKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGD 120
VYEN KMVHR VTYVPGLYKIF+EILVNAADNKQRDPSMDSVKV IDVE N ISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
Query: 121 GVPVEVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETAD 180
GVPVE+H EEKVY KKTTGGRNG GAKLTNIFST+F IETAD
Sbjct: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRLKKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
GKR KKYKQVF DNMG KSEP+ITKCK +NWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VVDLAGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPPKRYHVKPNERWEIVV 300
V+D+AGCLGKTVKVELNGQR+PV+SFQDYVDLYL S STF TEPPKRYH+KPNERWEIVV
Sbjct: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
Query: 301 ALSEGQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFWI 360
LSEGQF QV+FVN IATIKGGTHVDYITNQIANYV+NVVNKKNKNANVKAHNV SY W+
Sbjct: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FVNALIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKN--------------------- 420
F+NALIDNPAFDSQTKETLTIR S+FGSGC+LTPEFLKN
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
Query: 421 -----DG----------------------RTRCS--------------------GRDFYS 480
DG +C+ GRDFY
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHD 540
VFPLRG +LNVR SS++I +NKEIQ IKQILGLQ GKEYN+ K LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKENLR 557
GSHIKGLLINFIH FWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKE+LR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
BLAST of Sed0003769 vs. ExPASy TrEMBL
Match:
A0A6J1DIB5 (DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1)
HSP 1 Score: 944.1 bits (2439), Expect = 2.6e-271
Identity = 480/643 (74.65%), Postives = 509/643 (79.16%), Query Frame = 0
Query: 1 MPAEKKRPMQNSAAANGDPIPGGSNKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLW 60
M EKKRP+QNS AN D P G KTIEEMYQKKTQ+EHILLRPDTYVGS+E HTQ LW
Sbjct: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENGKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGD 120
VYEN KMVHR VTYVPGLYKIF+EILVNAADNKQRDPSMDSVKV IDVE N ISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
Query: 121 GVPVEVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETAD 180
GVPVE+H EEKVY KKTTGGRNG GAKLTNIFST+F IETAD
Sbjct: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRLKKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
GKR KKYKQVF DNMG KSEP+ITKCK +NWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VVDLAGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPPKRYHVKPNERWEIVV 300
V+D+AGCLGKTVKVELNGQR+PV+SFQDYVDLYL S STF TEPPKRYH+KPNERWEIVV
Sbjct: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
Query: 301 ALSEGQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFWI 360
LSEGQF QV+FVN IATIKGGTHVDYITNQIANYV+NVVNKKNKNANVKAHNV SY W+
Sbjct: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FVNALIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKN--------------------- 420
F+NALIDNPAFDSQTKETLTIR S+FGSGC+LTPEFLKN
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
Query: 421 -----DG----------------------RTRCS--------------------GRDFYS 480
DG +C+ GRDFY
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHD 540
VFPLRG +LNVR SS++I +NKEIQ IKQILGLQ GKEYN+ K LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKENLR 557
GSHIKGLLINFIH FWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKE+LR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
BLAST of Sed0003769 vs. ExPASy TrEMBL
Match:
A0A6J1F9E0 (DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1)
HSP 1 Score: 937.9 bits (2423), Expect = 1.9e-269
Identity = 474/643 (73.72%), Postives = 511/643 (79.47%), Query Frame = 0
Query: 1 MPAEKKRPMQNSAAANGDPIPGGSNKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLW 60
M EKKRP+QNS AAN D P G KTIEEMYQKKTQ+EHILLRPDTYVGS+E HTQ LW
Sbjct: 1 MAVEKKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENGKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGD 120
VYEN KMVHR VTYVPGLYKIF+EILVNAADNKQRDPSMDS+KV ID E N+ISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120
Query: 121 GVPVEVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETAD 180
GVPVEVH EEKVY KKTTGGRNG GAKLTNIFST+F IETAD
Sbjct: 121 GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRLKKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
G+RLKKYKQVF+DNMG KSEPIITKCK +NWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VVDLAGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPPKRYHVKPNERWEIVV 300
V+DLAGCLGKTVKVELNGQR+PV+SFQDYVD YL S + F TEPPKRYH++PNERWEIVV
Sbjct: 241 VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300
Query: 301 ALSEGQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFWI 360
LSEGQF QV+FVNGIATIKGGTHVDYITNQIANYV+ VVNKKNKNANVKAHNV SY W+
Sbjct: 301 TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FVNALIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKN--------------------- 420
F+NALIDNPAFDSQTKETLTIR S+FGS C+LTP+FLKN
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420
Query: 421 -----DG----------------------RTRCS--------------------GRDFYS 480
DG +C+ GRDFY
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHD 540
VFPLRG +LNVR SS++I +NKEIQ IKQILGLQ GKEYN+AK+LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKENLR 557
GSHIKGLLINFIH FWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK+NLR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600
BLAST of Sed0003769 vs. ExPASy TrEMBL
Match:
A0A6J1F3D6 (DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1)
HSP 1 Score: 937.9 bits (2423), Expect = 1.9e-269
Identity = 474/643 (73.72%), Postives = 511/643 (79.47%), Query Frame = 0
Query: 1 MPAEKKRPMQNSAAANGDPIPGGSNKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLW 60
M EKKRP+QNS AAN D P G KTIEEMYQKKTQ+EHILLRPDTYVGS+E HTQ LW
Sbjct: 1 MAVEKKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENGKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGD 120
VYEN KMVHR VTYVPGLYKIF+EILVNAADNKQRDPSMDS+KV ID E N+ISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120
Query: 121 GVPVEVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETAD 180
GVPVEVH EEKVY KKTTGGRNG GAKLTNIFST+F IETAD
Sbjct: 121 GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRLKKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
G+RLKKYKQVF+DNMG KSEPIITKCK +NWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VVDLAGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPPKRYHVKPNERWEIVV 300
V+DLAGCLGKTVKVELNGQR+PV+SFQDYVD YL S + F TEPPKRYH++PNERWEIVV
Sbjct: 241 VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300
Query: 301 ALSEGQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFWI 360
LSEGQF QV+FVNGIATIKGGTHVDYITNQIANYV+ VVNKKNKNANVKAHNV SY W+
Sbjct: 301 TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FVNALIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKN--------------------- 420
F+NALIDNPAFDSQTKETLTIR S+FGS C+LTP+FLKN
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420
Query: 421 -----DG----------------------RTRCS--------------------GRDFYS 480
DG +C+ GRDFY
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHD 540
VFPLRG +LNVR SS++I +NKEIQ IKQILGLQ GKEYN+AK+LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKENLR 557
GSHIKGLLINFIH FWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK+NLR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600
BLAST of Sed0003769 vs. ExPASy TrEMBL
Match:
A0A6J1J555 (DNA topoisomerase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481402 PE=3 SV=1)
HSP 1 Score: 932.6 bits (2409), Expect = 7.9e-268
Identity = 471/641 (73.48%), Postives = 509/641 (79.41%), Query Frame = 0
Query: 3 AEKKRPMQNSAAANGDPIPGGSNKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLWVY 62
A +KRP+QNS AAN D P G KTIEEMYQKKTQ+EHILLRPDTYVGS+E HTQ LW+Y
Sbjct: 2 AVEKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIY 61
Query: 63 ENGKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGDGV 122
EN KMVHR VTYVPGLYKIF+EILVNAADNKQRDPSMDS+KV ID E N ISVFNNGDGV
Sbjct: 62 ENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNCISVFNNGDGV 121
Query: 123 PVEVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETADGK 182
PVEVH EEKVY KKTTGGRNG GAKLTNIFST+F IETADG+
Sbjct: 122 PVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETADGR 181
Query: 183 RLKKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKRVV 242
RLKKYKQVF+DNMG KSEPIITKCK +NWTKVTFKPDLAKFNMTHLEDDVVALMKKRV+
Sbjct: 182 RLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKRVL 241
Query: 243 DLAGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPPKRYHVKPNERWEIVVAL 302
DLAGCLGKTVKVELNGQR+PV+SFQDYVD YL S + F TEPPKRYH++PNERWEIVV L
Sbjct: 242 DLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVVTL 301
Query: 303 SEGQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFWIFV 362
SEGQF QV+FVNGIATIKGGTHVDYITNQIANYV+ VVNKKNKNANVKAHNV SY W+F+
Sbjct: 302 SEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWVFI 361
Query: 363 NALIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKN----------------------- 422
NALIDNPAFDSQTKETLTIR S+FGS C+LTP+FLKN
Sbjct: 362 NALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSKDL 421
Query: 423 ---DG----------------------RTRCS--------------------GRDFYSVF 482
DG +C+ GRDFY VF
Sbjct: 422 KKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYGVF 481
Query: 483 PLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHDGS 542
PLRG +LNVR SS++I +NKEIQ IKQILGLQ GKEYN+AK+LRYGHLMIMTDQDHDGS
Sbjct: 482 PLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHDGS 541
Query: 543 HIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKENLRGN 557
HIKGLLINFIH FWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK+NLRGN
Sbjct: 542 HIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLRGN 601
BLAST of Sed0003769 vs. TAIR 10
Match:
AT3G23890.1 (topoisomerase II )
HSP 1 Score: 768.8 bits (1984), Expect = 2.9e-222
Identity = 401/644 (62.27%), Postives = 466/644 (72.36%), Query Frame = 0
Query: 6 KRPMQNSAAANGDPIPGGS-----NKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLW 65
K P+QNS AAN P S KTIEEMYQKK+Q+EHILLRPDTY+GS+E HTQTLW
Sbjct: 4 KLPLQNSNAANVAKAPAKSRAAAGGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLW 63
Query: 66 VYENGKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGD 125
VYE +MV R VTYVPGLYKIF+EILVNAADNKQRD MDSV+V IDVE N ISV N+G
Sbjct: 64 VYEKDEMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGA 123
Query: 126 GVPVEVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETAD 185
GVPVE+H EE +Y KKTTGGRNG GAKLTNIFST+F IETAD
Sbjct: 124 GVPVEIHQEEGIYVPEMIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETAD 183
Query: 186 GKRLKKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKR 245
GKRLKKYKQVF +NMG KSEP+ITKC +ENWTKVTFKPDL KFNMT LEDDVVALM KR
Sbjct: 184 GKRLKKYKQVFENNMGKKSEPVITKCNKSENWTKVTFKPDLKKFNMTELEDDVVALMSKR 243
Query: 246 VVDLAGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPP-KRYHVKPNERWEIV 305
V D+AGCLGK+VKVELNG++IPV+SF DYVDLYL++ + TE P R K N+RWE+
Sbjct: 244 VFDIAGCLGKSVKVELNGKQIPVKSFTDYVDLYLSAANKSRTEDPLPRLTEKVNDRWEVC 303
Query: 306 VALSEGQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFW 365
V+LSEGQF QV+FVN IATIKGGTHVDY+T+QI N++V VNKKNKNANVKAHNV ++ W
Sbjct: 304 VSLSEGQFQQVSFVNSIATIKGGTHVDYVTSQITNHIVAAVNKKNKNANVKAHNVKNHLW 363
Query: 366 IFVNALIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKNDGRT---------------- 425
+FVNALIDNPAFDSQTKETLT+R S+FGS C+L+ +FLK G++
Sbjct: 364 VFVNALIDNPAFDSQTKETLTLRQSSFGSKCELSEDFLKKVGKSGVVENLLSWADFKQNK 423
Query: 426 ------------------------------RCS--------------------GRDFYSV 485
C+ G ++ V
Sbjct: 424 DLKKSDGAKTGRVLVEKLDDAAEAGGKNSRLCTLILTEGDSAKSLALAGRSVLGNNYCGV 483
Query: 486 FPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHDG 545
FPLRG +LNVR S+ +I NKEI+ +K+ILGL+ +Y + SLRYG +MIMTDQDHDG
Sbjct: 484 FPLRGKLLNVREASTTQITNNKEIENLKKILGLKQNMKYENVNSLRYGQMMIMTDQDHDG 543
Query: 546 SHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGT--VLSFYTMPEYESWKENL 557
SHIKGLLINFIH FWPSLL+VPSFLVEFITPIVKAT R GT VLSFY+MPEYE WKE+L
Sbjct: 544 SHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKAT-RKGTKKVLSFYSMPEYEEWKESL 603
BLAST of Sed0003769 vs. TAIR 10
Match:
AT3G23890.2 (topoisomerase II )
HSP 1 Score: 768.8 bits (1984), Expect = 2.9e-222
Identity = 401/644 (62.27%), Postives = 466/644 (72.36%), Query Frame = 0
Query: 6 KRPMQNSAAANGDPIPGGS-----NKTIEEMYQKKTQVEHILLRPDTYVGSVENHTQTLW 65
K P+QNS AAN P S KTIEEMYQKK+Q+EHILLRPDTY+GS+E HTQTLW
Sbjct: 4 KLPLQNSNAANVAKAPAKSRAAAGGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLW 63
Query: 66 VYENGKMVHRCVTYVPGLYKIFNEILVNAADNKQRDPSMDSVKVEIDVEGNWISVFNNGD 125
VYE +MV R VTYVPGLYKIF+EILVNAADNKQRD MDSV+V IDVE N ISV N+G
Sbjct: 64 VYEKDEMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGA 123
Query: 126 GVPVEVHLEEKVY-------------------KKTTGGRNGLGAKLTNIFSTQFFIETAD 185
GVPVE+H EE +Y KKTTGGRNG GAKLTNIFST+F IETAD
Sbjct: 124 GVPVEIHQEEGIYVPEMIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETAD 183
Query: 186 GKRLKKYKQVFNDNMGSKSEPIITKCKATENWTKVTFKPDLAKFNMTHLEDDVVALMKKR 245
GKRLKKYKQVF +NMG KSEP+ITKC +ENWTKVTFKPDL KFNMT LEDDVVALM KR
Sbjct: 184 GKRLKKYKQVFENNMGKKSEPVITKCNKSENWTKVTFKPDLKKFNMTELEDDVVALMSKR 243
Query: 246 VVDLAGCLGKTVKVELNGQRIPVRSFQDYVDLYLNSVSTFPTEPP-KRYHVKPNERWEIV 305
V D+AGCLGK+VKVELNG++IPV+SF DYVDLYL++ + TE P R K N+RWE+
Sbjct: 244 VFDIAGCLGKSVKVELNGKQIPVKSFTDYVDLYLSAANKSRTEDPLPRLTEKVNDRWEVC 303
Query: 306 VALSEGQFHQVTFVNGIATIKGGTHVDYITNQIANYVVNVVNKKNKNANVKAHNVNSYFW 365
V+LSEGQF QV+FVN IATIKGGTHVDY+T+QI N++V VNKKNKNANVKAHNV ++ W
Sbjct: 304 VSLSEGQFQQVSFVNSIATIKGGTHVDYVTSQITNHIVAAVNKKNKNANVKAHNVKNHLW 363
Query: 366 IFVNALIDNPAFDSQTKETLTIRHSNFGSGCDLTPEFLKNDGRT---------------- 425
+FVNALIDNPAFDSQTKETLT+R S+FGS C+L+ +FLK G++
Sbjct: 364 VFVNALIDNPAFDSQTKETLTLRQSSFGSKCELSEDFLKKVGKSGVVENLLSWADFKQNK 423
Query: 426 ------------------------------RCS--------------------GRDFYSV 485
C+ G ++ V
Sbjct: 424 DLKKSDGAKTGRVLVEKLDDAAEAGGKNSRLCTLILTEGDSAKSLALAGRSVLGNNYCGV 483
Query: 486 FPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSLRYGHLMIMTDQDHDG 545
FPLRG +LNVR S+ +I NKEI+ +K+ILGL+ +Y + SLRYG +MIMTDQDHDG
Sbjct: 484 FPLRGKLLNVREASTTQITNNKEIENLKKILGLKQNMKYENVNSLRYGQMMIMTDQDHDG 543
Query: 546 SHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGT--VLSFYTMPEYESWKENL 557
SHIKGLLINFIH FWPSLL+VPSFLVEFITPIVKAT R GT VLSFY+MPEYE WKE+L
Sbjct: 544 SHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKAT-RKGTKKVLSFYSMPEYEEWKESL 603
BLAST of Sed0003769 vs. TAIR 10
Match:
AT5G04130.1 (DNA GYRASE B2 )
HSP 1 Score: 70.9 bits (172), Expect = 3.8e-12
Identity = 53/151 (35.10%), Postives = 79/151 (52.32%), Query Frame = 0
Query: 382 GRTRCSGRD--FYSVFPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAK-- 441
G + GRD F ++ PLRG ILN+ K + KN+EIQ + ILGL G + D K
Sbjct: 524 GGSAKQGRDRRFQAILPLRGKILNIERKDEAAMYKNEEIQNL--ILGLGLGVKGEDFKKE 583
Query: 442 SLRYGHLMIMTDQDHDGSHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGTVL 501
+LRY ++I+TD D DG+HI+ LL+ F + +L V + P+ K R
Sbjct: 584 NLRYHKIIILTDADVDGAHIRTLLLTFFFRYQRALFDAGCIYVG-VPPLFK-VERGKNAQ 643
Query: 502 SFYTMPEYESWKENLRGNSSGWSIKYYKGLG 529
Y + + N N+S ++I+ +KGLG
Sbjct: 644 YCYDDADLKKITSNFPANAS-YNIQRFKGLG 669
BLAST of Sed0003769 vs. TAIR 10
Match:
AT3G10270.1 (DNA GYRASE B1 )
HSP 1 Score: 67.8 bits (164), Expect = 3.2e-11
Identity = 50/149 (33.56%), Postives = 77/149 (51.68%), Query Frame = 0
Query: 382 GRTRCSGRD--FYSVFPLRGVILNVRVKSSEEIAKNKEIQEIKQILGLQHGKEYNDAKSL 441
G + GRD F ++ PLRG ILN+ K + KN+EIQ + LGL E + ++L
Sbjct: 564 GGSAKQGRDRRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFNKENL 623
Query: 442 RYGHLMIMTDQDHDGSHIKGLLINFIHYFWPSLLRVPSFLVEFITPIVKATHRNGTVLSF 501
RY ++I+TD D DG+HI+ LL+ F + +L V + P+ K R
Sbjct: 624 RYHKIIILTDADVDGAHIRTLLLTFFFRYQRALFDAGCIYVG-VPPLFK-VERGKQAHYC 683
Query: 502 YTMPEYESWKENLRGNSSGWSIKYYKGLG 529
Y + + GN+S ++I+ +KGLG
Sbjct: 684 YDDAALKKITASFPGNAS-YNIQRFKGLG 709
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022152631.1 | 5.4e-271 | 74.65 | DNA topoisomerase 2 isoform X1 [Momordica charantia] | [more] |
| XP_022152632.1 | 5.4e-271 | 74.65 | DNA topoisomerase 2 isoform X2 [Momordica charantia] | [more] |
| XP_023527826.1 | 1.0e-269 | 73.72 | DNA topoisomerase 2 isoform X6 [Cucurbita pepo subsp. pepo] | [more] |
| XP_023527825.1 | 1.0e-269 | 73.72 | DNA topoisomerase 2 isoform X5 [Cucurbita pepo subsp. pepo] | [more] |
| XP_023527823.1 | 1.0e-269 | 73.72 | DNA topoisomerase 2 isoform X3 [Cucurbita pepo subsp. pepo] >XP_023527824.1 DNA ... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| O24308 | 1.3e-222 | 61.85 | DNA topoisomerase 2 OS=Pisum sativum OX=3888 GN=TOP2 PE=2 SV=1 | [more] |
| P30182 | 4.1e-221 | 62.27 | DNA topoisomerase 2 OS=Arabidopsis thaliana OX=3702 GN=TOP2 PE=2 SV=2 | [more] |
| P08096 | 6.6e-163 | 47.99 | DNA topoisomerase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28... | [more] |
| Q55BP5 | 9.6e-154 | 47.50 | Probable DNA topoisomerase 2 OS=Dictyostelium discoideum OX=44689 GN=top2 PE=3 S... | [more] |
| O46374 | 6.4e-150 | 48.87 | DNA topoisomerase 2-alpha OS=Sus scrofa OX=9823 GN=TOP2A PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DGT3 | 2.6e-271 | 74.65 | DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1 | [more] |
| A0A6J1DIB5 | 2.6e-271 | 74.65 | DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1 | [more] |
| A0A6J1F9E0 | 1.9e-269 | 73.72 | DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1 | [more] |
| A0A6J1F3D6 | 1.9e-269 | 73.72 | DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1 | [more] |
| A0A6J1J555 | 7.9e-268 | 73.48 | DNA topoisomerase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481402 PE=3 SV=1 | [more] |
