Sed0003766 (gene) Chayote v1

Overview
NameSed0003766
Typegene
OrganismSechium edule (Chayote v1)
DescriptionEf-hand calcium binding protein
LocationLG14: 1417895 .. 1422703 (-)
RNA-Seq ExpressionSed0003766
SyntenySed0003766
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATTATGAAAAAATCATACCCTCATTGGTAATTTGGCATGAATGTCACTTTTGTAATAATATTCAAATTCATGTCGAGAATCACATCTTAAACCCTCCACCGTCAGAAGACTTATTACACAACTGCTTGGGATCAGCACCTTCAATTTCCATTTGCGTTTTTGTTACCAGAGATTAAAAAAAAAAAAAAAATGTCAGGTTACCCAAATCAGCCCGCAGGTTACGGTTACGGCTACGGCGCCGGCGGTGGCTACGGCGCCGGCGGTGGCTTCGGTGCTCCTCCACCGACGGCTCAGCCCTACGCTCCGGTGGCCGCCCCATACGGCGCACCGTCGGCACCGGGCGAGAAACCGCACAAGAACAAGCCCGATCAGTCTCACGGCGGAGCAGGCGGCGGTCATCAGTCTTACGGCGGAGCAGGCGGCGGTCATCAGTCTTACGGCGGATCAGGCGGTGGTCATCAGGCCTACGGCGGCGCAGGCGGCGGTTATCCACCGCCTTCTGCGTACGGAAACCCTTTCGCGTCTCTCATGCCGTCGGCGTTTCCGCCTGGTACAGATCCGAGCATCGTTGCGTGTTTTCAAGTCGCCGATCAGGATGGGAGCGGGTTTATTGACGATAAGGAGTTGCAAGGTGTTCTTTCTTCGTATAATCAGAAGTTTAGCATCAGAACTGTTCATCTTCTCATGTATCATTTCACGAACACGAACACGAGGAAGATCGGTAACCTCGCATTTCCTTTTCTCTATGGCCTTTTGTTGTTTGTACTAGCAATAGGAAATTTCTGTGAGTTTAGATTCTTAAATCAACAACAGTAATGATGAAAAAAAATGAAGAAATGAGAGCTTTTGAGAATCTTGAAGGCCCAGATTAGAGAAAAAAAGGCTAACAGTTTGGTGTTTGGATGAGGAGTTAGCTTGATGAGTAATTTTGGCTAGTTAGACATAGAAAGTTGCAATAAAAAACTCGTTTGGATGGAGTTAGGCAATAGTTTGTTTGTTTGGATGTGTATTTGAAATTAGTAGTTATTTTGAGAAGTTATAAATGAATGATTAGAAATTAGTGCATAAAAATGAGTTATTTTGGGTAGTTAGAAATGAAGGATTAAAAATGAGTTATTTTGAATAGTTAGAAATGAACGATTAAAAATTGGTGGTTAGAAATGAATAGTTATTTTGAATCGTTAGAAATTAATGATTAGAAATGAGTGGCTAGAAATGAATAGTTATTTTGAGTAGTTAGAAATGAATGATTAGAAATGAGTGGTTGGAAATGAGGAGTTATTTTTAGTATTAAAAATTAAAAATGAGGAGTTGTTTTGAGTACTTACAAAAAAATGCGTATGAATGTGGTTTTTTGAGGAATAGTTAGTAAATATTCATTTTAAATCTTCCATAAATTCAACAAACATACACCCCCTACTATTAGTTTCAACTACTTTTAACAATTATAAATTTATGTTTCTTAATTGAACGGATTGGAACAGGTCAGACATTTGGTGTTGAAACCATTTATGTGAATTGCATTATAATTGCTTTGAATTGTATTTTATGTTTTAGTGATTGTGATGTGATTTTCTCTTATTAGTTGATTAGTTCATGAAGTTCATAAATCATATCTTTGAGTTATTCTCTAAGATACTAAATTTTTTCTAATACTATTTCTATTTTAGACAAGGATAACTCAGTTTTATTTTAAGTTTGTTTGATCTGGTGTACCCAAAAGTTATTTTTAAAAAAATAAATTGAAACCTACTATTTGGACTTGTATTAGTCGTGGTGGTTGCACAAATTTAACTAGTTTCAAACTTGTGGTTAAAGTTATTGACTTTTCAATACTCGAAGCTTTTGGGTTATTATAATCTGTCAATTTGGAAGTATTTTATTTATTATTTTTTTTATAATTTATGCAAAACAGGGACGTTGTCTATTGAAATAGAGTATGTAGTTCAATTTCCATTACTTTATTGACATTTTTTATATATAGATTTGACATTGAGAGGTTGAAAACTCTGCCTTAGATTTGATATATGCTGTGTTGTAAGCTTTTCTATTGACATTTTTTATTGCATTTCTTTTCTGAAAAACAGGACCCAAGGAATTCATTGCTCTGTTCTATGGTCTTCAGAGCTGGAGGGTAAACTGAATATTTGATATTATTTTAGCCAACATTATCTGCTGGTGTAATTCTATGAAATAGAGTATTTAATGCATCTTTTTTCAGGGTATTTTTGAGAGATTCGATTCAGATAGAAGTGGAAGAATTGATTCGAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGGTTTGCAGTCTCACCGATGGTCTTGGACTTGCTGGTGTCCAAGTTTGACAAAAGTGGTGGAAAAAGCAAGGCCATTGAATATGATAATTTCATTGAGTACGTTTGTTTCCCTTGCTCTTGATTTGACATGACGCTTTTTTAAAGAAAAAATTAGAGATTTCATGTGATATTTCTAAGCTTCTGGGCTGTTTGTTTGACATATTTAGGCAGTATTCAGGTTAAGGAGTTATATTGAGAAGTTAAAAATTGAGAGTTGTGTCAAAAACTCGTTTGGATGTAGTTTTTGTGTAAAGGAGTTAGAGTCAAAACCTTGTTTGGATGTAGATTTAAAAATGAGTAGTTATTCTTAGTAGTTAAAAAAGAGATGATTTTAAAATGAATGGTTAAAAATGAGTTATTTTGATTGATATGAAAGAATGATTAAAAATAAGTGGTTAGAAATGATTGAGTAGTTATAAATGAATGATTAAAAATGAGAGTTATTTTTAAAAGTAAAAATGAGGAGAGTTAATAAAAACACGTTTGGATGTAAGTTTTCGAGGAGGAGTTAGTAAATCCTAAAACTAACTCCTACCTAAACATGTCGAACAAAATGCCCCTAAAGTTTTCATTGATCACTGTCCTGATTGTTTTTTCTTTTTCTAATGCAGGTGTTGTCTAACAGTCAAGGTGAGTTTTTGACTATATTTATTTTCATATATCTTTTTCATATTATAGTATATACTGTTTAGAAGTGGTGACGCCATCTTTGGAACCTGAACTTGATTTTATTCCATTTATGTTTTTTTTGTGAGAAAAAGCTAATTCATTAAAAAGCAACTCTGAGGCAAACCTCCATAGCAAGTACAATACCAGGCGAGGGGCAAACCCCCCCCCCCCCCCCAACACAAACCCTAAAAAACACACAAAAATAAAGGCTCCTCACAAACACCACAGCGCAAACATGGATACTAAGAAAATCATAACCACCAAACCACGGACATACCATCTGTGGACTTCAAAGAGTCCGATTGCAGCTGCCCAAAGAGTCCGATTTTATTCCATTTATGTTATGCTTATAAATACGTTGAATGCATGTTACAACTTACACTATTTTAACTCTAGTGGTTATAAAGTAATCACTTTTTTTTAATACCGTGAGAGTCGGATGGGCTAGGCCAGCTTATAAATATCGCATCTCAACTACTCTCATGAGAAAAATCGCCTAACCTTACAATATTTGGCTGTGAAGGAAACTTCTAGGAAGTTAATTCTTAGGTCACTTTGGATTGAATTCATAACTTCTTAGATTCCTAAAAGGCTAACATTGTTTATGAGTCATGACCATTTGGTCAACCCATGATGGTTATCAAGCAGTCACTTTCTGATAAGAACCAAATGTATATTTAAATAAAAGGTACTTGGATCCTCCCAATCTTGAGATCACTCTCAAGCCTTGGATACTCAATATCTCAATACTTTTCAATATCTCAATACTTTTCAATATCTCCCTTCTTTTCTATTCGCTCTTATTTGTAGTCTAATATTCTTATTCTACTTCTAATAGAATTGACCATTCCTTATCCTACTCTTAATAGGATTGACTATTCCTTATCCTACTCCTAAACGAGCCTTAACATATCTACTAAAATATCTAATGGTCAAACTAGCCGTTATGTGGGATTCTTATCAGCCCTCCCTACCCCTTCTACGGTACACTTACATGATATGATAAGGGGTCTATCACTTTCATCATTGAATTTCGCTTTGAGAAGCCGTTTGTATTTTCCCTATAACATGCAATGTTTCTTTTCTTTTTTTCACACGCTGGTATTATAAACTCCTTGTATGAACTCTTGACTCAATTGTCCAGCAACCTCAACATATAATCATTGTTGATTTTTGTGGCTACCCATAATTCATATGAACATTTTGATGTGGGGAGAATGATAGGCTTAAACTGAAGTGCATTTGATGTAAAAAAAAATTACAGGGACTGACTGAGAAATTCAAGGAAAAGGACACCACATACTCTGGGTCTGCAACATTCAGTTATGAGGCTTTCATGCTGACCGTCCTGCCTTTCCTCATCGCCTGAGGAGTTTTTCAGACTCCGTTCCGGCATGGGTGTTCACTGTTTCGTGTAAAGCTCGGGTTTTTTTTCGGTTTTTTCTTTTCGATATCCGTTGTGTTCGATAGAATCTATATCTTCTCTATGTAGTCTTATCCTTATCAACGCTCTTTGTTTTTGTTTATGTATAAGTTGGCTATCTCAAGTATACAATGGGAGTTCTATGAATGCCCAACGCTGTTAACTGTTTCCTTTCCGGCTTTACAATTTTGCATTTCACATTAAAATTCAAGTCAATTGTATAATACCAATGTGCATGTAATTATCGAGCATTCTTGTTCTCAAATGAACAAGAGTCGAATGGAATTAGTGATCGATCGTAATAAGAATTATGCGGAGGATCTTACCTTAAATATGTGATGACATCATTTTTAAGACTAGAATTTTAGTGCGGCG

mRNA sequence

CAATTATGAAAAAATCATACCCTCATTGGTAATTTGGCATGAATGTCACTTTTGTAATAATATTCAAATTCATGTCGAGAATCACATCTTAAACCCTCCACCGTCAGAAGACTTATTACACAACTGCTTGGGATCAGCACCTTCAATTTCCATTTGCGTTTTTGTTACCAGAGATTAAAAAAAAAAAAAAAATGTCAGGTTACCCAAATCAGCCCGCAGGTTACGGTTACGGCTACGGCGCCGGCGGTGGCTACGGCGCCGGCGGTGGCTTCGGTGCTCCTCCACCGACGGCTCAGCCCTACGCTCCGGTGGCCGCCCCATACGGCGCACCGTCGGCACCGGGCGAGAAACCGCACAAGAACAAGCCCGATCAGTCTCACGGCGGAGCAGGCGGCGGTCATCAGTCTTACGGCGGAGCAGGCGGCGGTCATCAGTCTTACGGCGGATCAGGCGGTGGTCATCAGGCCTACGGCGGCGCAGGCGGCGGTTATCCACCGCCTTCTGCGTACGGAAACCCTTTCGCGTCTCTCATGCCGTCGGCGTTTCCGCCTGGTACAGATCCGAGCATCGTTGCGTGTTTTCAAGTCGCCGATCAGGATGGGAGCGGGTTTATTGACGATAAGGAGTTGCAAGGTGTTCTTTCTTCGTATAATCAGAAGTTTAGCATCAGAACTGTTCATCTTCTCATGTATCATTTCACGAACACGAACACGAGGAAGATCGGACCCAAGGAATTCATTGCTCTGTTCTATGGTCTTCAGAGCTGGAGGGGTATTTTTGAGAGATTCGATTCAGATAGAAGTGGAAGAATTGATTCGAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGGTTTGCAGTCTCACCGATGGTCTTGGACTTGCTGGTGTCCAAGTTTGACAAAAGTGGTGGAAAAAGCAAGGCCATTGAATATGATAATTTCATTGAGTGTTGTCTAACAGTCAAGGGACTGACTGAGAAATTCAAGGAAAAGGACACCACATACTCTGGGTCTGCAACATTCAGTTATGAGGCTTTCATGCTGACCGTCCTGCCTTTCCTCATCGCCTGAGGAGTTTTTCAGACTCCGTTCCGGCATGGGTGTTCACTGTTTCGTGTAAAGCTCGGGTTTTTTTTCGGTTTTTTCTTTTCGATATCCGTTGTGTTCGATAGAATCTATATCTTCTCTATGTAGTCTTATCCTTATCAACGCTCTTTGTTTTTGTTTATGTATAAGTTGGCTATCTCAAGTATACAATGGGAGTTCTATGAATGCCCAACGCTGTTAACTGTTTCCTTTCCGGCTTTACAATTTTGCATTTCACATTAAAATTCAAGTCAATTGTATAATACCAATGTGCATGTAATTATCGAGCATTCTTGTTCTCAAATGAACAAGAGTCGAATGGAATTAGTGATCGATCGTAATAAGAATTATGCGGAGGATCTTACCTTAAATATGTGATGACATCATTTTTAAGACTAGAATTTTAGTGCGGCG

Coding sequence (CDS)

ATGTCAGGTTACCCAAATCAGCCCGCAGGTTACGGTTACGGCTACGGCGCCGGCGGTGGCTACGGCGCCGGCGGTGGCTTCGGTGCTCCTCCACCGACGGCTCAGCCCTACGCTCCGGTGGCCGCCCCATACGGCGCACCGTCGGCACCGGGCGAGAAACCGCACAAGAACAAGCCCGATCAGTCTCACGGCGGAGCAGGCGGCGGTCATCAGTCTTACGGCGGAGCAGGCGGCGGTCATCAGTCTTACGGCGGATCAGGCGGTGGTCATCAGGCCTACGGCGGCGCAGGCGGCGGTTATCCACCGCCTTCTGCGTACGGAAACCCTTTCGCGTCTCTCATGCCGTCGGCGTTTCCGCCTGGTACAGATCCGAGCATCGTTGCGTGTTTTCAAGTCGCCGATCAGGATGGGAGCGGGTTTATTGACGATAAGGAGTTGCAAGGTGTTCTTTCTTCGTATAATCAGAAGTTTAGCATCAGAACTGTTCATCTTCTCATGTATCATTTCACGAACACGAACACGAGGAAGATCGGACCCAAGGAATTCATTGCTCTGTTCTATGGTCTTCAGAGCTGGAGGGGTATTTTTGAGAGATTCGATTCAGATAGAAGTGGAAGAATTGATTCGAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGGTTTGCAGTCTCACCGATGGTCTTGGACTTGCTGGTGTCCAAGTTTGACAAAAGTGGTGGAAAAAGCAAGGCCATTGAATATGATAATTTCATTGAGTGTTGTCTAACAGTCAAGGGACTGACTGAGAAATTCAAGGAAAAGGACACCACATACTCTGGGTCTGCAACATTCAGTTATGAGGCTTTCATGCTGACCGTCCTGCCTTTCCTCATCGCCTGA

Protein sequence

MSGYPNQPAGYGYGYGAGGGYGAGGGFGAPPPTAQPYAPVAAPYGAPSAPGEKPHKNKPDQSHGGAGGGHQSYGGAGGGHQSYGGSGGGHQAYGGAGGGYPPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Homology
BLAST of Sed0003766 vs. NCBI nr
Match: XP_008464601.1 (PREDICTED: probable calcium-binding protein CML49 [Cucumis melo] >TYK04310.1 putative calcium-binding protein CML49 [Cucumis melo var. makuwa])

HSP 1 Score: 472.2 bits (1214), Expect = 3.2e-129
Identity = 256/312 (82.05%), Postives = 265/312 (84.94%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYGYGAGGGYGAGGGFGAPPPTA----------------QPYAPVAAPY 60
           MSGYPNQP+GYGYGY    GYG  GG+GAPPPTA                QPYAPVAAPY
Sbjct: 1   MSGYPNQPSGYGYGY----GYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPY 60

Query: 61  GAPSAP----GEKPHKNKPDQSHGGAGGGHQSYGGAGGGHQSYGGSGGGHQAYGGAGGGY 120
           G PSAP    GEKP K+KP+QS+GGAGGGHQSYGGA GG                 G GY
Sbjct: 61  GGPSAPYSAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGG-----------------GHGY 120

Query: 121 PPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIR 180
           PPPSAYGNPFASL+PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIR
Sbjct: 121 PPPSAYGNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIR 180

Query: 181 TVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALLSLG 240
           TVHLLMYHFTNTNTRKIGPKEFI+LFYGLQSWRGIFERFDSDRSG+IDSNELR+ALLSLG
Sbjct: 181 TVHLLMYHFTNTNTRKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLG 240

Query: 241 FAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE 293
           FAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE
Sbjct: 241 FAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE 291

BLAST of Sed0003766 vs. NCBI nr
Match: XP_022153892.1 (probable calcium-binding protein CML49 isoform X1 [Momordica charantia] >XP_022153893.1 probable calcium-binding protein CML49 isoform X1 [Momordica charantia] >XP_022153894.1 probable calcium-binding protein CML49 isoform X1 [Momordica charantia] >XP_022153895.1 probable calcium-binding protein CML49 isoform X1 [Momordica charantia])

HSP 1 Score: 470.7 bits (1210), Expect = 9.2e-129
Identity = 255/315 (80.95%), Postives = 266/315 (84.44%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYGYGAGGGYGAGGGFGAPPPTAQP------------------YAPVAA 60
           MSGYPNQP+GYGYGYG  G  G  GG+GAPPPTAQP                  YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60

Query: 61  PYGAPSAP-----GEKPHKNKPDQSHGGAGGGHQSYGGAGGGHQSYGGSGGGHQAYGGAG 120
           PYGAPSAP     G+KP K+KP          HQSYGGAGGGHQSYGG+GGGH       
Sbjct: 61  PYGAPSAPYGSSTGDKPPKDKP----------HQSYGGAGGGHQSYGGAGGGH------- 120

Query: 121 GGYPPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKF 180
             YPPP++YGNPFASL+PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKF
Sbjct: 121 -AYPPPNSYGNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKF 180

Query: 181 SIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALL 240
           SIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSG+IDSNELREALL
Sbjct: 181 SIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALL 240

Query: 241 SLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATF 293
           SLGFAVSP+VLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATF
Sbjct: 241 SLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATF 297

BLAST of Sed0003766 vs. NCBI nr
Match: XP_038883807.1 (probable calcium-binding protein CML50 [Benincasa hispida])

HSP 1 Score: 468.8 bits (1205), Expect = 3.5e-128
Identity = 256/314 (81.53%), Postives = 264/314 (84.08%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYGYGAGGGYGAGGGFGAPPPTA-----------------QPYAPVAAP 60
           MSGYPNQP+GYGYGY    GYG  GG+GAPPPTA                 QPYAPVAAP
Sbjct: 1   MSGYPNQPSGYGYGY----GYGGSGGYGAPPPTAQVYNSSAYGHPQPQQTQQPYAPVAAP 60

Query: 61  YGAP-----SAPGEKPHKNKPDQSHGGAGGGHQSYGGAGGGHQSYGGSGGGHQAYGGAGG 120
           YGAP     SAPGEKP K+KP+QS+GGAGGGHQSYGGAG G                 G 
Sbjct: 61  YGAPSAPYGSAPGEKPPKDKPNQSYGGAGGGHQSYGGAGSG-----------------GH 120

Query: 121 GYPPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFS 180
           GYPPPSAYGNPFASL+PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQ VLSSY+QKFS
Sbjct: 121 GYPPPSAYGNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQRVLSSYDQKFS 180

Query: 181 IRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALLS 240
           IRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSG+IDS ELREALLS
Sbjct: 181 IRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSKELREALLS 240

Query: 241 LGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFS 293
           LGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFS
Sbjct: 241 LGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFS 293

BLAST of Sed0003766 vs. NCBI nr
Match: XP_004142653.1 (probable calcium-binding protein CML49 [Cucumis sativus] >KGN54506.1 hypothetical protein Csa_011963 [Cucumis sativus])

HSP 1 Score: 465.3 bits (1196), Expect = 3.9e-127
Identity = 254/313 (81.15%), Postives = 263/313 (84.03%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYGYGAGGGYGAGGGFGAPPPTA----------------QPYAPVAAPY 60
           MSGYPNQP+GYGYGYG+ GGY      GAPPPTA                QPYAPVAAPY
Sbjct: 1   MSGYPNQPSGYGYGYGSTGGY------GAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPY 60

Query: 61  GAPSAP-----GEKPHKNKPDQSHGGAGGGHQSYGGAGGGHQSYGGSGGGHQAYGGAGGG 120
           G PSAP     GEKP K+KP+QS+GGAGGGHQSYGGA  G                 G G
Sbjct: 61  GVPSAPYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASG-----------------GHG 120

Query: 121 YPPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSI 180
           YPPPSAYG+PFASL+PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSI
Sbjct: 121 YPPPSAYGSPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSI 180

Query: 181 RTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALLSL 240
           RTVHLLMYHFTNTNTRKIGPKEFI+LFYGLQSWRGIFERFDSDRSG+IDSNELREALLSL
Sbjct: 181 RTVHLLMYHFTNTNTRKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSL 240

Query: 241 GFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSY 293
           GFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSY
Sbjct: 241 GFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSY 290

BLAST of Sed0003766 vs. NCBI nr
Match: XP_023518609.1 (probable calcium-binding protein CML49 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 462.2 bits (1188), Expect = 3.3e-126
Identity = 250/309 (80.91%), Postives = 256/309 (82.85%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYGYGAGGGYGAG------------GGFGAPPPTAQPYAPVAAPYGAPS 60
           MSGYPNQP+GYGYGYG  GGYGA             G    PP + QPYAPVAAPYGAPS
Sbjct: 1   MSGYPNQPSGYGYGYGGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYGAPS 60

Query: 61  A-----PGEKPHKNKPDQSHGGAGGGHQSYGGAGGGHQSYGGSGGGHQAYGGAGGGYPPP 120
           A     PGEKPHK+KP+QSHGG GG HQSYG                      G GYPPP
Sbjct: 61  APYGSNPGEKPHKDKPNQSHGGVGGSHQSYG----------------------GHGYPPP 120

Query: 121 SAYGNPFASLMPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVH 180
           SAYG+PFASL+PSAFPPGTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVH
Sbjct: 121 SAYGSPFASLLPSAFPPGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVH 180

Query: 181 LLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALLSLGFAV 240
           LLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRID NELREALLSLGFAV
Sbjct: 181 LLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAV 240

Query: 241 SPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFM 293
           SPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFM
Sbjct: 241 SPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFM 287

BLAST of Sed0003766 vs. ExPASy Swiss-Prot
Match: Q8W4L0 (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 PE=2 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 2.4e-95
Identity = 213/335 (63.58%), Postives = 238/335 (71.04%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYG---------YGAGGG----YGAGGGFGAPP-------PTAQPYAPV 60
           MSGYP    GYGYG         YG+ G     YG+ G    PP       P A PY   
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSASSPYAVPYGAQ 60

Query: 61  AAPYGAP------SAPGE--KPHKNKP------DQSHGGAG----GGHQSYGGAGGGHQ- 120
            APYGAP      S PG+  KPHK KP        S GG G     G   YGG GG  Q 
Sbjct: 61  PAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQ 120

Query: 121 -SYGGSGGGHQAYGGAGGGY---PPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDG 180
             +GG  GG     G GGGY   PP ++YG+PFASL+PSAFPPGTDP+IVACFQ AD+D 
Sbjct: 121 SGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDN 180

Query: 181 SGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFE 240
           SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ LQ+WR IFE
Sbjct: 181 SGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNWRSIFE 240

Query: 241 RFDSDRSGRIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTV 293
           RFD DRSGRID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYDNFIECCLTV
Sbjct: 241 RFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIECCLTV 300

BLAST of Sed0003766 vs. ExPASy Swiss-Prot
Match: Q2QY10 (Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.2e-91
Identity = 199/305 (65.25%), Postives = 223/305 (73.11%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYGYGAGGGYGAGGGFGAPPPTAQPYAPVAAPYGA-PSAPGEKPHKNKP 60
           M+GYP  P G GY YG  GGYGA      PPP     AP A PYGA P   G+    + P
Sbjct: 1   MAGYPPNP-GSGYPYGGAGGYGA-----PPPPYGSSPAPSAPPYGAKPPKEGKTSSSSAP 60

Query: 61  DQSHGGAGGGHQSYGGAGGGHQSYGGSGG-----GHQAYGGAGGGYPP-------PSAYG 120
              + G GGG+    GA    Q YG  GG       Q YG   G  PP       P  YG
Sbjct: 61  ---YYGGGGGY----GAPPSTQPYGSGGGYGAPPSSQPYGAPYGAPPPSSAPYGAPGGYG 120

Query: 121 NPFASLMPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMY 180
           +PFASL+PSAFPPGTDP++VACFQ AD+DGSG IDDKELQ  LS Y+Q FS+RTVHLLMY
Sbjct: 121 SPFASLVPSAFPPGTDPNVVACFQAADRDGSGMIDDKELQSALSGYSQSFSLRTVHLLMY 180

Query: 181 HFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALLSLGFAVSPMV 240
            FTNTN RKIGPKEF ++FY LQ+WR IFERFD DRSG+ID+ ELR+ALLSLG++VSP V
Sbjct: 181 LFTNTNVRKIGPKEFTSVFYSLQNWRSIFERFDRDRSGKIDATELRDALLSLGYSVSPTV 240

Query: 241 LDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVL 293
           LDLLVSKFDK+GGK+KAIEYDNFIECCLTVKGLTEKFKEKDT +SGSATF+YEAFMLTVL
Sbjct: 241 LDLLVSKFDKTGGKNKAIEYDNFIECCLTVKGLTEKFKEKDTAFSGSATFTYEAFMLTVL 292

BLAST of Sed0003766 vs. ExPASy Swiss-Prot
Match: Q9FYE4 (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 2.7e-91
Identity = 211/357 (59.10%), Postives = 232/357 (64.99%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYGYGAG------------GG-----------------YGA-------- 60
           MSGYP    GYGYGYG G            GG                 YGA        
Sbjct: 1   MSGYPPTSQGYGYGYGGGNQPPPPQPPYSSGGNNPPYGSSTTSSPYAVPYGASKPQSSSS 60

Query: 61  ------GGGFGAPPPTAQPYAPVAAPYGAPSAPGEKPH---------KNKPDQSHGGAGG 120
                    +GAPPP+A PYAP    Y  P  P EKP+             D    GAG 
Sbjct: 61  SAPTYGSSSYGAPPPSA-PYAPSPGDYNKP--PKEKPYGGGYGAPPPSGSSDYGSYGAGP 120

Query: 121 GHQSYGGAGGGH--------QSYGGSGG-----GHQAYGGAGGGYPPPSAYGNPFASLMP 180
                 G GGG+          YG  GG         +GG  GGYPP ++YG+PFASL+P
Sbjct: 121 RPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPFASLIP 180

Query: 181 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 240
           S F PGTDP+IVACFQ ADQDGSGFIDDKELQG LSSY Q+FS+RTVHLLMY FTN+N  
Sbjct: 181 SGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNSNAM 240

Query: 241 KIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALLSLGFAVSPMVLDLLVSKF 293
           KIGPKEF ALFY LQ+WR IFER D DRSGRID NELR+ALLSLGF+VSP+VLDLLVSKF
Sbjct: 241 KIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDLLVSKF 300

BLAST of Sed0003766 vs. ExPASy Swiss-Prot
Match: Q9ZQH1 (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 PE=2 SV=2)

HSP 1 Score: 145.2 bits (365), Expect = 1.2e-33
Identity = 85/205 (41.46%), Postives = 122/205 (59.51%), Query Frame = 0

Query: 89  GHQAYGGAGGGYPPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQG 148
           G   Y  A   Y P   +     S     F P T P IV  F+ AD++ SGF+++ EL+ 
Sbjct: 25  GESRYTYAYPSYQPTQQF-----SSYSGMFSPETHPEIVRSFESADRNRSGFLEESELRQ 84

Query: 149 VLS-SYNQKFSIRTVHLLM--YHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSG 208
            LS S     S RT+ LL+  Y     +  ++GPKE++ L+  L  WR IF R+D DRSG
Sbjct: 85  ALSLSGYDGISNRTIRLLLFIYKIPVDSLLRLGPKEYVELWNCLAQWRAIFNRYDRDRSG 144

Query: 209 RIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFK 268
           +++S +LR+A  +LG  +   V  L+VS+FD   GK+  + +D+F+EC + VKGLTEKF+
Sbjct: 145 KMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDDGTGKTVDLCFDSFLECGMIVKGLTEKFR 204

Query: 269 EKDTTYSGSATFSYEAFMLTVLPFL 291
           E D  Y+G AT SY+ FML V+PF+
Sbjct: 205 ENDPGYTGYATLSYDVFMLMVIPFI 224

BLAST of Sed0003766 vs. ExPASy Swiss-Prot
Match: Q6DC93 (Peflin OS=Danio rerio OX=7955 GN=pef1 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.6e-25
Identity = 99/285 (34.74%), Postives = 147/285 (51.58%), Query Frame = 0

Query: 11  YGYGYGAGGGYGAGGGFGAPPPTAQPYAPVAAPYGAP--SAPGEKPHKNKPDQSHGGAGG 70
           Y YG G  G  G    +  PP       P A  YG+P  SAP        P Q +GG G 
Sbjct: 3   YQYGQGYSGPGGNAPQWQQPPRAPYAGGPAAGQYGSPYGSAP--------PGQQYGG-GS 62

Query: 71  GHQSYGGAGGGHQSYGGSGGGHQAYGGAGGGYPPPSAYGNPFASLMPSA-FPPGTDPSIV 130
            + SY G  G    YGG     QA GG  GGY  P   G P+     +   PPG +P   
Sbjct: 63  PYGSY-GQPGPRAPYGGG----QAPGGPYGGYGQPQ--GGPYRQQGSAGNVPPGVNPEAY 122

Query: 131 ACFQVADQDGSGFIDDKELQGVLSSY-NQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALF 190
             F   D D SG+I+ KEL+  L ++ N  F+  T  +++  F  T + ++    F AL+
Sbjct: 123 QWFSTVDSDQSGYINAKELKQALMNFNNSSFNDETCIMMLNMFDKTKSGRVDVFGFSALW 182

Query: 191 YGLQSWRGIFERFDSDRSGRIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIE 250
             LQ WR  F++FD DRSG I++NE+ +AL  +G+ +SP  +  LV+++   GG +  ++
Sbjct: 183 TFLQQWRAAFQQFDRDRSGSINTNEMHQALSQMGYNLSPQFIQELVNRYSVRGG-TGVLQ 242

Query: 251 YDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLI 292
            D FI+ C  ++ +T+ F+EKDT  +G+   SYE F+ + +  L+
Sbjct: 243 LDRFIQVCTQLQSMTQAFREKDTGMTGNVRMSYEDFLSSAITRLM 270

BLAST of Sed0003766 vs. ExPASy TrEMBL
Match: A0A5D3BYW0 (Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold527G00390 PE=4 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.5e-129
Identity = 256/312 (82.05%), Postives = 265/312 (84.94%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYGYGAGGGYGAGGGFGAPPPTA----------------QPYAPVAAPY 60
           MSGYPNQP+GYGYGY    GYG  GG+GAPPPTA                QPYAPVAAPY
Sbjct: 1   MSGYPNQPSGYGYGY----GYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPY 60

Query: 61  GAPSAP----GEKPHKNKPDQSHGGAGGGHQSYGGAGGGHQSYGGSGGGHQAYGGAGGGY 120
           G PSAP    GEKP K+KP+QS+GGAGGGHQSYGGA GG                 G GY
Sbjct: 61  GGPSAPYSAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGG-----------------GHGY 120

Query: 121 PPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIR 180
           PPPSAYGNPFASL+PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIR
Sbjct: 121 PPPSAYGNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIR 180

Query: 181 TVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALLSLG 240
           TVHLLMYHFTNTNTRKIGPKEFI+LFYGLQSWRGIFERFDSDRSG+IDSNELR+ALLSLG
Sbjct: 181 TVHLLMYHFTNTNTRKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLG 240

Query: 241 FAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE 293
           FAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE
Sbjct: 241 FAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE 291

BLAST of Sed0003766 vs. ExPASy TrEMBL
Match: A0A1S3CNE3 (probable calcium-binding protein CML49 OS=Cucumis melo OX=3656 GN=LOC103502445 PE=4 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.5e-129
Identity = 256/312 (82.05%), Postives = 265/312 (84.94%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYGYGAGGGYGAGGGFGAPPPTA----------------QPYAPVAAPY 60
           MSGYPNQP+GYGYGY    GYG  GG+GAPPPTA                QPYAPVAAPY
Sbjct: 1   MSGYPNQPSGYGYGY----GYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPY 60

Query: 61  GAPSAP----GEKPHKNKPDQSHGGAGGGHQSYGGAGGGHQSYGGSGGGHQAYGGAGGGY 120
           G PSAP    GEKP K+KP+QS+GGAGGGHQSYGGA GG                 G GY
Sbjct: 61  GGPSAPYSAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGG-----------------GHGY 120

Query: 121 PPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIR 180
           PPPSAYGNPFASL+PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIR
Sbjct: 121 PPPSAYGNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIR 180

Query: 181 TVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALLSLG 240
           TVHLLMYHFTNTNTRKIGPKEFI+LFYGLQSWRGIFERFDSDRSG+IDSNELR+ALLSLG
Sbjct: 181 TVHLLMYHFTNTNTRKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLG 240

Query: 241 FAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE 293
           FAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE
Sbjct: 241 FAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE 291

BLAST of Sed0003766 vs. ExPASy TrEMBL
Match: A0A6J1DIS2 (probable calcium-binding protein CML49 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021303 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.4e-129
Identity = 255/315 (80.95%), Postives = 266/315 (84.44%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYGYGAGGGYGAGGGFGAPPPTAQP------------------YAPVAA 60
           MSGYPNQP+GYGYGYG  G  G  GG+GAPPPTAQP                  YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60

Query: 61  PYGAPSAP-----GEKPHKNKPDQSHGGAGGGHQSYGGAGGGHQSYGGSGGGHQAYGGAG 120
           PYGAPSAP     G+KP K+KP          HQSYGGAGGGHQSYGG+GGGH       
Sbjct: 61  PYGAPSAPYGSSTGDKPPKDKP----------HQSYGGAGGGHQSYGGAGGGH------- 120

Query: 121 GGYPPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKF 180
             YPPP++YGNPFASL+PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKF
Sbjct: 121 -AYPPPNSYGNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKF 180

Query: 181 SIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALL 240
           SIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSG+IDSNELREALL
Sbjct: 181 SIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALL 240

Query: 241 SLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATF 293
           SLGFAVSP+VLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATF
Sbjct: 241 SLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATF 297

BLAST of Sed0003766 vs. ExPASy TrEMBL
Match: A0A0A0L336 (Ef-hand calcium binding protein OS=Cucumis sativus OX=3659 GN=Csa_4G341560 PE=4 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 1.9e-127
Identity = 254/313 (81.15%), Postives = 263/313 (84.03%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYGYGAGGGYGAGGGFGAPPPTA----------------QPYAPVAAPY 60
           MSGYPNQP+GYGYGYG+ GGY      GAPPPTA                QPYAPVAAPY
Sbjct: 1   MSGYPNQPSGYGYGYGSTGGY------GAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPY 60

Query: 61  GAPSAP-----GEKPHKNKPDQSHGGAGGGHQSYGGAGGGHQSYGGSGGGHQAYGGAGGG 120
           G PSAP     GEKP K+KP+QS+GGAGGGHQSYGGA  G                 G G
Sbjct: 61  GVPSAPYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASG-----------------GHG 120

Query: 121 YPPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSI 180
           YPPPSAYG+PFASL+PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSI
Sbjct: 121 YPPPSAYGSPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSI 180

Query: 181 RTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALLSL 240
           RTVHLLMYHFTNTNTRKIGPKEFI+LFYGLQSWRGIFERFDSDRSG+IDSNELREALLSL
Sbjct: 181 RTVHLLMYHFTNTNTRKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSL 240

Query: 241 GFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSY 293
           GFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSY
Sbjct: 241 GFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSY 290

BLAST of Sed0003766 vs. ExPASy TrEMBL
Match: A0A6J1HP98 (probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC111466073 PE=4 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 3.5e-126
Identity = 249/309 (80.58%), Postives = 256/309 (82.85%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYGYGAGGGYGAG------------GGFGAPPPTAQPYAPVAAPYGAPS 60
           MSGYPNQP+GYGYGYG  GGYGA             G    PP + QPYAPVAAPYGAPS
Sbjct: 1   MSGYPNQPSGYGYGYGGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYGAPS 60

Query: 61  A-----PGEKPHKNKPDQSHGGAGGGHQSYGGAGGGHQSYGGSGGGHQAYGGAGGGYPPP 120
           A     PGEKPHK+KP+QSHGG GG HQSYG                      G GYPPP
Sbjct: 61  APYGSNPGEKPHKDKPNQSHGGVGGSHQSYG----------------------GHGYPPP 120

Query: 121 SAYGNPFASLMPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVH 180
           SAYG+PFASL+PSAFPPGTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVH
Sbjct: 121 SAYGSPFASLLPSAFPPGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVH 180

Query: 181 LLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALLSLGFAV 240
           LLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSG+ID NELREALLSLGFAV
Sbjct: 181 LLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFAV 240

Query: 241 SPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFM 293
           SPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFM
Sbjct: 241 SPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFM 287

BLAST of Sed0003766 vs. TAIR 10
Match: AT3G10300.3 (Calcium-binding EF-hand family protein )

HSP 1 Score: 350.1 bits (897), Expect = 1.7e-96
Identity = 213/335 (63.58%), Postives = 238/335 (71.04%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYG---------YGAGGG----YGAGGGFGAPP-------PTAQPYAPV 60
           MSGYP    GYGYG         YG+ G     YG+ G    PP       P A PY   
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSASSPYAVPYGAQ 60

Query: 61  AAPYGAP------SAPGE--KPHKNKP------DQSHGGAG----GGHQSYGGAGGGHQ- 120
            APYGAP      S PG+  KPHK KP        S GG G     G   YGG GG  Q 
Sbjct: 61  PAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQ 120

Query: 121 -SYGGSGGGHQAYGGAGGGY---PPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDG 180
             +GG  GG     G GGGY   PP ++YG+PFASL+PSAFPPGTDP+IVACFQ AD+D 
Sbjct: 121 SGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDN 180

Query: 181 SGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFE 240
           SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ LQ+WR IFE
Sbjct: 181 SGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNWRSIFE 240

Query: 241 RFDSDRSGRIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTV 293
           RFD DRSGRID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYDNFIECCLTV
Sbjct: 241 RFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIECCLTV 300

BLAST of Sed0003766 vs. TAIR 10
Match: AT5G04170.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 336.7 bits (862), Expect = 1.9e-92
Identity = 211/357 (59.10%), Postives = 232/357 (64.99%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYGYGAG------------GG-----------------YGA-------- 60
           MSGYP    GYGYGYG G            GG                 YGA        
Sbjct: 1   MSGYPPTSQGYGYGYGGGNQPPPPQPPYSSGGNNPPYGSSTTSSPYAVPYGASKPQSSSS 60

Query: 61  ------GGGFGAPPPTAQPYAPVAAPYGAPSAPGEKPH---------KNKPDQSHGGAGG 120
                    +GAPPP+A PYAP    Y  P  P EKP+             D    GAG 
Sbjct: 61  SAPTYGSSSYGAPPPSA-PYAPSPGDYNKP--PKEKPYGGGYGAPPPSGSSDYGSYGAGP 120

Query: 121 GHQSYGGAGGGH--------QSYGGSGG-----GHQAYGGAGGGYPPPSAYGNPFASLMP 180
                 G GGG+          YG  GG         +GG  GGYPP ++YG+PFASL+P
Sbjct: 121 RPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPFASLIP 180

Query: 181 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 240
           S F PGTDP+IVACFQ ADQDGSGFIDDKELQG LSSY Q+FS+RTVHLLMY FTN+N  
Sbjct: 181 SGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNSNAM 240

Query: 241 KIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDSNELREALLSLGFAVSPMVLDLLVSKF 293
           KIGPKEF ALFY LQ+WR IFER D DRSGRID NELR+ALLSLGF+VSP+VLDLLVSKF
Sbjct: 241 KIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDLLVSKF 300

BLAST of Sed0003766 vs. TAIR 10
Match: AT3G10300.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 282.7 bits (722), Expect = 3.3e-76
Identity = 179/295 (60.68%), Postives = 202/295 (68.47%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYG---------YGAGGG----YGAGGGFGAPP-------PTAQPYAPV 60
           MSGYP    GYGYG         YG+ G     YG+ G    PP       P A PY   
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSASSPYAVPYGAQ 60

Query: 61  AAPYGAP------SAPGE--KPHKNKP------DQSHGGAG----GGHQSYGGAGGGHQ- 120
            APYGAP      S PG+  KPHK KP        S GG G     G   YGG GG  Q 
Sbjct: 61  PAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQ 120

Query: 121 -SYGGSGGGHQAYGGAGGGY---PPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDG 180
             +GG  GG     G GGGY   PP ++YG+PFASL+PSAFPPGTDP+IVACFQ AD+D 
Sbjct: 121 SGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDN 180

Query: 181 SGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFE 240
           SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ LQ+WR IFE
Sbjct: 181 SGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNWRSIFE 240

Query: 241 RFDSDRSGRIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIE 253
           RFD DRSGRID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYDNFIE
Sbjct: 241 RFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

BLAST of Sed0003766 vs. TAIR 10
Match: AT3G10300.4 (Calcium-binding EF-hand family protein )

HSP 1 Score: 282.7 bits (722), Expect = 3.3e-76
Identity = 179/295 (60.68%), Postives = 202/295 (68.47%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYG---------YGAGGG----YGAGGGFGAPP-------PTAQPYAPV 60
           MSGYP    GYGYG         YG+ G     YG+ G    PP       P A PY   
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSASSPYAVPYGAQ 60

Query: 61  AAPYGAP------SAPGE--KPHKNKP------DQSHGGAG----GGHQSYGGAGGGHQ- 120
            APYGAP      S PG+  KPHK KP        S GG G     G   YGG GG  Q 
Sbjct: 61  PAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQ 120

Query: 121 -SYGGSGGGHQAYGGAGGGY---PPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDG 180
             +GG  GG     G GGGY   PP ++YG+PFASL+PSAFPPGTDP+IVACFQ AD+D 
Sbjct: 121 SGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDN 180

Query: 181 SGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFE 240
           SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ LQ+WR IFE
Sbjct: 181 SGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNWRSIFE 240

Query: 241 RFDSDRSGRIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIE 253
           RFD DRSGRID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYDNFIE
Sbjct: 241 RFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

BLAST of Sed0003766 vs. TAIR 10
Match: AT3G10300.5 (Calcium-binding EF-hand family protein )

HSP 1 Score: 278.1 bits (710), Expect = 8.1e-75
Identity = 179/296 (60.47%), Postives = 202/296 (68.24%), Query Frame = 0

Query: 1   MSGYPNQPAGYGYG---------YGAGGG----YGAGGGFGAPP-------PTAQPYAPV 60
           MSGYP    GYGYG         YG+ G     YG+ G    PP       P A PY   
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSASSPYAVPYGAQ 60

Query: 61  AAPYGAP------SAPGE--KPHKNKP------DQSHGGAG----GGHQSYGGAGGGHQ- 120
            APYGAP      S PG+  KPHK KP        S GG G     G   YGG GG  Q 
Sbjct: 61  PAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYGGAPQQ 120

Query: 121 -SYGGSGGGHQAYGGAGGGY---PPPSAYGNPFASLMPSAFPPGTDPSIVACFQVADQDG 180
             +GG  GG     G GGGY   PP ++YG+PFASL+PSAFPPGTDP+IVACFQ AD+D 
Sbjct: 121 SGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDN 180

Query: 181 SGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIG-PKEFIALFYGLQSWRGIF 240
           SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIG PKEF +LF+ LQ+WR IF
Sbjct: 181 SGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGRPKEFTSLFFSLQNWRSIF 240

Query: 241 ERFDSDRSGRIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIE 253
           ERFD DRSGRID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYDNFIE
Sbjct: 241 ERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008464601.13.2e-12982.05PREDICTED: probable calcium-binding protein CML49 [Cucumis melo] >TYK04310.1 put... [more]
XP_022153892.19.2e-12980.95probable calcium-binding protein CML49 isoform X1 [Momordica charantia] >XP_0221... [more]
XP_038883807.13.5e-12881.53probable calcium-binding protein CML50 [Benincasa hispida][more]
XP_004142653.13.9e-12781.15probable calcium-binding protein CML49 [Cucumis sativus] >KGN54506.1 hypothetica... [more]
XP_023518609.13.3e-12680.91probable calcium-binding protein CML49 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8W4L02.4e-9563.58Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 ... [more]
Q2QY101.2e-9165.25Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2... [more]
Q9FYE42.7e-9159.10Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 ... [more]
Q9ZQH11.2e-3341.46Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 ... [more]
Q6DC932.6e-2534.74Peflin OS=Danio rerio OX=7955 GN=pef1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BYW01.5e-12982.05Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CNE31.5e-12982.05probable calcium-binding protein CML49 OS=Cucumis melo OX=3656 GN=LOC103502445 P... [more]
A0A6J1DIS24.4e-12980.95probable calcium-binding protein CML49 isoform X1 OS=Momordica charantia OX=3673... [more]
A0A0A0L3361.9e-12781.15Ef-hand calcium binding protein OS=Cucumis sativus OX=3659 GN=Csa_4G341560 PE=4 ... [more]
A0A6J1HP983.5e-12680.58probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC1114660... [more]
Match NameE-valueIdentityDescription
AT3G10300.31.7e-9663.58Calcium-binding EF-hand family protein [more]
AT5G04170.11.9e-9259.10Calcium-binding EF-hand family protein [more]
AT3G10300.23.3e-7660.68Calcium-binding EF-hand family protein [more]
AT3G10300.43.3e-7660.68Calcium-binding EF-hand family protein [more]
AT3G10300.58.1e-7560.47Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 125..153
e-value: 0.092
score: 21.9
coord: 191..219
e-value: 0.019
score: 24.2
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 126..149
e-value: 5.8E-5
score: 22.4
coord: 196..214
e-value: 3.2E-4
score: 20.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 121..156
score: 10.691735
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 187..222
score: 12.644413
NoneNo IPR availableGENE3D1.10.238.10coord: 104..291
e-value: 1.5E-39
score: 137.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..90
NoneNo IPR availablePANTHERPTHR46824:SF1CALCIUM-BINDING PROTEIN CML49-RELATEDcoord: 1..292
NoneNo IPR availableCDDcd16180EFh_PEF_Group_Icoord: 125..289
e-value: 1.3504E-75
score: 226.256
IPR044590Probable calcium-binding protein CML48/49/50PANTHERPTHR46824CALCIUM-BINDING PROTEIN CML48-RELATEDcoord: 1..292
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 134..146
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 200..212
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 119..287

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0003766.1Sed0003766.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding