Sed0003727 (gene) Chayote v1

Overview


Name	Sed0003727
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Eukaryotic aspartyl protease family protein
Location	LG04: 31781604 .. 31783693 (+)
RNA-Seq Expression	Sed0003727
Synteny	Sed0003727

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTAACAAATGATCGGTTATTGAAAATGACATTTGAAAAATAAGTTATTATAACAAGAATTATAATAAACTATGTTGGAGAGATTATAATAAATATATATGTATCTATAGTTTAGATAAAGAGCACATAAATTTAGGTAACGGGACAAATATAGGCTGTTATTATAATTAACAGATCGATTTACATAAACGCGGCACAAAGACATATTAAAGAGTTTATGTGAAAAAGAAAGAAGCTCCCGTTAGTTCTAATGGCCACTAGTCTTGGAACCCATACAAAATCCTACCACTTCTTCAAACCAGAACCTTCTTTCTTCTTCTTCTTCTTTTCTTCTTCTTCTTCAATTCCCTTCTCTTTATATTCCAAACTTCATCTCTCTCTCTCTCTGTCTATCCTCTTCAATGGCTTCCCCTGTTTTTGCATTCCTCTGTTTTCTCCTTTCTTCTTCCTCTGTTTTCTCTTCACAAATTCTCCTTCTCCCTCTCTCCCATTCCTTATCATCCAACCTCAACAACAACAACACCCACAACCTCCTCAAATCCGCCGCCGACCGCTCCTCCGCCCGCCACCGCCGCCGCCGCCGCACCCACCTCTCCCTCCCCCTGTCCCCCGGCGGCGACTACACTCTCTCCTTCAACCTGGGCTCCCACGCTCACACCATTTCCCTCTACATGGACACCGGCAGCGACTTCGTCTGGTTCCCCTGTTCCCCCTTCGAGTGTATTCTCTGCGAAGGCAAGCCCCAAATTCAACCCCCCTTTCCCCAAATTCCCAAACAAAAATCTGTTTCTTGCAGCGCCCCCGCCTGCTCCGCCGCCCACGGCGCCTCCCTCTCCGCCTCCCACCTCTGCGCTATTTCCCGATGCCCCCTCGAATCCATTGAAGTTTCTGAGTGCTCTTCCTTTTCTTGTCCGCCTCTGTATTACGCTTACGGCGATGGGAGTTTGATTGCTCAGCTTTATAGAGACAGCTTGGGTTTGCCGGCGCCGGCGCCCTCACCGCCGATTCGTGTTCGGAATTTCACTTTCGGGTGTGCACATACGGCGCTCGGGGAGCCGGTCGGGGTTGCTGGGTTCGGCCGGGGGGCTTTGTCGATGCCGAGTCAGTTAGCTACTTTCTCACCTCAATTAGGGAACCGCTTTTCTTATTGCTTGGTTTCTCACTCCTTTAATACGGACCGGGTTCGCCGACCGAGTCCGTTGATTCTGGGCCGGTATTCTGATGGCCAGTCGGAGTTTGTTTACACGTCTTTGCTTGATAATCCGAAGCACCCTTATTTTTACTCGGTTGGATTGGCCGGAATCTCGGTCGGCACAGTGACGATTCCGGCGCCGGAGTTTTTGAAAAGGGTGGACGACGGCGGCAGCGGTGGTGTTGTGGTGGATTCCGGTACTACTTTCACTATGCTCCCGGCGGGTTTGTATGAATCGGTTGTGGCTCAGTTTGAGAATCGGACCGGTCGGGTTGCAACGCGGGCGAGCCGGATTGAAGAAAATACCGGGTTGAGTCCTTGCTATTATTATGATGACTCAATTCAAGTGCCACGTGTCGTGCTGCATTTCGTCGGGGAAAGATCTAGTGTGGTGCTTCCCAGGAAGAACTACTTTTACGAGTTTTTTGACGGTGGAGATGGGGTGGGGAAGAAGAGAAAAGTTGGGTGTTTGATGTTGATGAACGGCGGAGATGAGGCTGAGCTGGCAGGTGGGCCCGGGGCCACACTAGGCAACTATCAACAACAGGGCTTTGAAGTGGTTTACGATTTGGAGAAAAACCGGGTCGGTTTTGCCCGGCGACAGTGTTCAACTCTTTGGGACAGCCTGAACCGAAGTTAATGTGAAATGTGGGACCGGTTCGAGGACTGGAAGGTTGACTATTTTGTGCTTTGACTTTGTTGTAGTTGCAATAGTCCACGCTTTTTGGAGTAAATGAGGTAATTTGACGTTTGATGTGGGCCTTCTTTTGTAAATTCTTGCTAGCACTATACTTCTTTGCTTCATTGTTATTTATTATAGTTGAAATTTGTACGTGAAAAGTGTTATAAATGTTCATAAACACAAGTGGGAAAATATTATTTATTTTAAGGCAGGA

mRNA sequence

ATTTTAACAAATGATCGGTTATTGAAAATGACATTTGAAAAATAAGTTATTATAACAAGAATTATAATAAACTATGTTGGAGAGATTATAATAAATATATATGTATCTATAGTTTAGATAAAGAGCACATAAATTTAGGTAACGGGACAAATATAGGCTGTTATTATAATTAACAGATCGATTTACATAAACGCGGCACAAAGACATATTAAAGAGTTTATGTGAAAAAGAAAGAAGCTCCCGTTAGTTCTAATGGCCACTAGTCTTGGAACCCATACAAAATCCTACCACTTCTTCAAACCAGAACCTTCTTTCTTCTTCTTCTTCTTTTCTTCTTCTTCTTCAATTCCCTTCTCTTTATATTCCAAACTTCATCTCTCTCTCTCTCTGTCTATCCTCTTCAATGGCTTCCCCTGTTTTTGCATTCCTCTGTTTTCTCCTTTCTTCTTCCTCTGTTTTCTCTTCACAAATTCTCCTTCTCCCTCTCTCCCATTCCTTATCATCCAACCTCAACAACAACAACACCCACAACCTCCTCAAATCCGCCGCCGACCGCTCCTCCGCCCGCCACCGCCGCCGCCGCCGCACCCACCTCTCCCTCCCCCTGTCCCCCGGCGGCGACTACACTCTCTCCTTCAACCTGGGCTCCCACGCTCACACCATTTCCCTCTACATGGACACCGGCAGCGACTTCGTCTGGTTCCCCTGTTCCCCCTTCGAGTGTATTCTCTGCGAAGGCAAGCCCCAAATTCAACCCCCCTTTCCCCAAATTCCCAAACAAAAATCTGTTTCTTGCAGCGCCCCCGCCTGCTCCGCCGCCCACGGCGCCTCCCTCTCCGCCTCCCACCTCTGCGCTATTTCCCGATGCCCCCTCGAATCCATTGAAGTTTCTGAGTGCTCTTCCTTTTCTTGTCCGCCTCTGTATTACGCTTACGGCGATGGGAGTTTGATTGCTCAGCTTTATAGAGACAGCTTGGGTTTGCCGGCGCCGGCGCCCTCACCGCCGATTCGTGTTCGGAATTTCACTTTCGGGTGTGCACATACGGCGCTCGGGGAGCCGGTCGGGGTTGCTGGGTTCGGCCGGGGGGCTTTGTCGATGCCGAGTCAGTTAGCTACTTTCTCACCTCAATTAGGGAACCGCTTTTCTTATTGCTTGGTTTCTCACTCCTTTAATACGGACCGGGTTCGCCGACCGAGTCCGTTGATTCTGGGCCGGTATTCTGATGGCCAGTCGGAGTTTGTTTACACGTCTTTGCTTGATAATCCGAAGCACCCTTATTTTTACTCGGTTGGATTGGCCGGAATCTCGGTCGGCACAGTGACGATTCCGGCGCCGGAGTTTTTGAAAAGGGTGGACGACGGCGGCAGCGGTGGTGTTGTGGTGGATTCCGGTACTACTTTCACTATGCTCCCGGCGGGTTTGTATGAATCGGTTGTGGCTCAGTTTGAGAATCGGACCGGTCGGGTTGCAACGCGGGCGAGCCGGATTGAAGAAAATACCGGGTTGAGTCCTTGCTATTATTATGATGACTCAATTCAAGTGCCACGTGTCGTGCTGCATTTCGTCGGGGAAAGATCTAGTGTGGTGCTTCCCAGGAAGAACTACTTTTACGAGTTTTTTGACGGTGGAGATGGGGTGGGGAAGAAGAGAAAAGTTGGGTGTTTGATGTTGATGAACGGCGGAGATGAGGCTGAGCTGGCAGGTGGGCCCGGGGCCACACTAGGCAACTATCAACAACAGGGCTTTGAAGTGGTTTACGATTTGGAGAAAAACCGGGTCGGTTTTGCCCGGCGACAGTGTTCAACTCTTTGGGACAGCCTGAACCGAAGTTAATGTGAAATGTGGGACCGGTTCGAGGACTGGAAGGTTGACTATTTTGTGCTTTGACTTTGTTGTAGTTGCAATAGTCCACGCTTTTTGGAGTAAATGAGGTAATTTGACGTTTGATGTGGGCCTTCTTTTGTAAATTCTTGCTAGCACTATACTTCTTTGCTTCATTGTTATTTATTATAGTTGAAATTTGTACGTGAAAAGTGTTATAAATGTTCATAAACACAAGTGGGAAAATATTATTTATTTTAAGGCAGGA

Coding sequence (CDS)

ATGGCTTCCCCTGTTTTTGCATTCCTCTGTTTTCTCCTTTCTTCTTCCTCTGTTTTCTCTTCACAAATTCTCCTTCTCCCTCTCTCCCATTCCTTATCATCCAACCTCAACAACAACAACACCCACAACCTCCTCAAATCCGCCGCCGACCGCTCCTCCGCCCGCCACCGCCGCCGCCGCCGCACCCACCTCTCCCTCCCCCTGTCCCCCGGCGGCGACTACACTCTCTCCTTCAACCTGGGCTCCCACGCTCACACCATTTCCCTCTACATGGACACCGGCAGCGACTTCGTCTGGTTCCCCTGTTCCCCCTTCGAGTGTATTCTCTGCGAAGGCAAGCCCCAAATTCAACCCCCCTTTCCCCAAATTCCCAAACAAAAATCTGTTTCTTGCAGCGCCCCCGCCTGCTCCGCCGCCCACGGCGCCTCCCTCTCCGCCTCCCACCTCTGCGCTATTTCCCGATGCCCCCTCGAATCCATTGAAGTTTCTGAGTGCTCTTCCTTTTCTTGTCCGCCTCTGTATTACGCTTACGGCGATGGGAGTTTGATTGCTCAGCTTTATAGAGACAGCTTGGGTTTGCCGGCGCCGGCGCCCTCACCGCCGATTCGTGTTCGGAATTTCACTTTCGGGTGTGCACATACGGCGCTCGGGGAGCCGGTCGGGGTTGCTGGGTTCGGCCGGGGGGCTTTGTCGATGCCGAGTCAGTTAGCTACTTTCTCACCTCAATTAGGGAACCGCTTTTCTTATTGCTTGGTTTCTCACTCCTTTAATACGGACCGGGTTCGCCGACCGAGTCCGTTGATTCTGGGCCGGTATTCTGATGGCCAGTCGGAGTTTGTTTACACGTCTTTGCTTGATAATCCGAAGCACCCTTATTTTTACTCGGTTGGATTGGCCGGAATCTCGGTCGGCACAGTGACGATTCCGGCGCCGGAGTTTTTGAAAAGGGTGGACGACGGCGGCAGCGGTGGTGTTGTGGTGGATTCCGGTACTACTTTCACTATGCTCCCGGCGGGTTTGTATGAATCGGTTGTGGCTCAGTTTGAGAATCGGACCGGTCGGGTTGCAACGCGGGCGAGCCGGATTGAAGAAAATACCGGGTTGAGTCCTTGCTATTATTATGATGACTCAATTCAAGTGCCACGTGTCGTGCTGCATTTCGTCGGGGAAAGATCTAGTGTGGTGCTTCCCAGGAAGAACTACTTTTACGAGTTTTTTGACGGTGGAGATGGGGTGGGGAAGAAGAGAAAAGTTGGGTGTTTGATGTTGATGAACGGCGGAGATGAGGCTGAGCTGGCAGGTGGGCCCGGGGCCACACTAGGCAACTATCAACAACAGGGCTTTGAAGTGGTTTACGATTTGGAGAAAAACCGGGTCGGTTTTGCCCGGCGACAGTGTTCAACTCTTTGGGACAGCCTGAACCGAAGTTAA

Protein sequence

MASPVFAFLCFLLSSSSVFSSQILLLPLSHSLSSNLNNNNTHNLLKSAADRSSARHRRRRRTHLSLPLSPGGDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKPQIQPPFPQIPKQKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLYYAYGDGSLIAQLYRDSLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPSQLATFSPQLGNRFSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDNPKHPYFYSVGLAGISVGTVTIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGRVATRASRIEENTGLSPCYYYDDSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDGGDGVGKKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLNRS

Homology

[Close]

BLAST of Sed0003727 vs. NCBI nr
Match: XP_004147205.1 (probable aspartyl protease At4g16563 [Cucumis sativus])

HSP 1 Score: 826.6 bits (2134), Expect = 1.1e-235
Identity = 407/476 (85.50%), Postives = 433/476 (90.97%), Query Frame = 0


Query: 3   SPVFAFLCFLLSSSSVFSSQILLLPLSHSLSSNLNN-NNTHNLLKSAADRSSARHRRRRR 62
           SPVF FL   L SS VFSSQI LLPLSHSLSS++++ NNTHNLLKS A RSSAR  R R 
Sbjct: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHRHRH 63

Query: 63  THLSLPLSPGGDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKPQIQPPFP 122
            HLSLPLSPGGDYTLSFNLGS +H ISLYMDTGSD VWFPCSPFECILCEGKP+IQ P P
Sbjct: 64  NHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLP 123

Query: 123 QIPKQKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLYYAYGDGS 182
           +I   KSVSCSA ACSAAHG SLSASHLCAISRCPLESIE+SECSSFSCPP YYAYGDGS
Sbjct: 124 KIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGS 183

Query: 183 LIAQLYRDSLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPSQLATFSP 242
           L+A+LYRDSL LP PAPSPPI VRNFTFGCAHT LGEPVGVAGFGRG LSMPSQLATFSP
Sbjct: 184 LVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATFSP 243

Query: 243 QLGNRFSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDNPKHPYFYSVGLAGI 302
           QLGNRFSYCLVSHSF  DRVRRPSPLILGRY  G++EF+YTSLL+NPKHPYFYSVGLAGI
Sbjct: 244 QLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGLAGI 303

Query: 303 SVGTVTIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGRVATRASR 362
           SVG + IPAPEFL +VD+GGSGGVVVDSGTTFTMLPAGLYESVVA+FENRTG+VA RA R
Sbjct: 304 SVGNIRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANRARR 363

Query: 363 IEENTGLSPCYYYDDSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDGGDG-VGKKRKVGC 422
           IEENTGLSPCYYY++S+ VPRVVLHFVGE+S+VVLPRKNYFYEF DGGDG VG+KRKVGC
Sbjct: 364 IEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRKVGC 423

Query: 423 LMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLNRS 477
           LMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWD+LNRS
Sbjct: 424 LMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRS 479

[Close]

BLAST of Sed0003727 vs. NCBI nr
Match: XP_038905814.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 825.5 bits (2131), Expect = 2.4e-235
Identity = 408/481 (84.82%), Postives = 436/481 (90.64%), Query Frame = 0


Query: 1   MASPVFAFLCFLLSSSSVFSSQILLLPLSHSLSSNLNN-NNTHNLLKSAADRSSARHRRR 60
           MAS VF  L   L SS VFSSQ+LLLPLSHSLSS++++ NNTHNLLKS A RSSAR   R
Sbjct: 1   MASSVFVLLLCFLLSSPVFSSQLLLLPLSHSLSSSISDFNNTHNLLKSTAARSSARFHHR 60

Query: 61  RRT----HLSLPLSPGGDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKPQ 120
           RRT    HLSLPLSPGGDYTLSFNLGS +H ISLYMDTGSD VWFPCSPFECILCEGKP+
Sbjct: 61  RRTQHHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPK 120

Query: 121 IQPPFPQIPKQKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLYY 180
           +Q P P+I   KSVSCSAPACSAAHG SLSASHLCAIS+CPLESIE+SECSSFSCPP YY
Sbjct: 121 VQSPLPKISNNKSVSCSAPACSAAHGGSLSASHLCAISQCPLESIEISECSSFSCPPFYY 180

Query: 181 AYGDGSLIAQLYRDSLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPSQ 240
           AYGDGSLIA+LYRDSL LPAPAPSP I VRNFTFGCAHTALGEPVGVAGFGRG LSMPSQ
Sbjct: 181 AYGDGSLIARLYRDSLSLPAPAPSPAINVRNFTFGCAHTALGEPVGVAGFGRGTLSMPSQ 240

Query: 241 LATFSPQLGNRFSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDNPKHPYFYS 300
           LATFSPQLGNRFSYCLVSHSF  +RVRRPSPLILGRY  G++EF+YTSLL+NPKHPYFYS
Sbjct: 241 LATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFIYTSLLENPKHPYFYS 300

Query: 301 VGLAGISVGTVTIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGRV 360
           VGL GISVG + IPAPEFLK+VD+GGSGGVVVDSGTTFTMLPAGLY+SVVA FENRTGRV
Sbjct: 301 VGLTGISVGNMMIPAPEFLKKVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAAFENRTGRV 360

Query: 361 ATRASRIEENTGLSPCYYYDDSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDGGDGVGKK 420
           A RA RIEENTGLSPCYYY++S++VPRVVLHFVGE+SSV+LP+KNYFYEF DGGDGVGKK
Sbjct: 361 ANRARRIEENTGLSPCYYYENSVEVPRVVLHFVGEKSSVLLPKKNYFYEFLDGGDGVGKK 420

Query: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLNR 477
           RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDL KNRVGFARRQCSTLWDSLNR
Sbjct: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLAKNRVGFARRQCSTLWDSLNR 480

[Close]

BLAST of Sed0003727 vs. NCBI nr
Match: XP_008448851.1 (PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo])

HSP 1 Score: 823.5 bits (2126), Expect = 9.1e-235
Identity = 406/478 (84.94%), Postives = 433/478 (90.59%), Query Frame = 0


Query: 3   SPVFAFLCFLLSSSSVFSSQILLLPLSHSLSSNLNN-NNTHNLLKSAADRSSARHRRRRR 62
           SPVF FL   L SS VFSSQI LLPLSHSLSS++++ N+THNLLKS A RSSAR  R R 
Sbjct: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHRHRH 63

Query: 63  THLSLPLSPGGDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKPQIQPPFP 122
            HLSLPLSPGGDYTLSFNLGS +H ISLYMDTGSD VWFPCSPFECILCEGKP+IQ P P
Sbjct: 64  NHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLP 123

Query: 123 QIPKQKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLYYAYGDGS 182
           +I   KSVSCSA ACSAAHG SLSASHLCAISRCPLESIE+SECSSFSCPP YYAYGDGS
Sbjct: 124 KISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGS 183

Query: 183 LIAQLYRDSLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPSQLATFSP 242
           L+A+LYRDSL LP PAPSPPI VRNFTFGCAHT LGEPVGVAGFGRG LSMPSQLATFSP
Sbjct: 184 LVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATFSP 243

Query: 243 QLGNRFSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDNPKHPYFYSVGLAGI 302
           QLGNRFSYCLVSHSF  DRVRRPSPLILGRY  G++EF+YTSLL+NPKHPYFYSVGLAGI
Sbjct: 244 QLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGLAGI 303

Query: 303 SVGTVTIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGRVATRASR 362
           SVG V IPAPEFL++VD+ GSGGVVVDSGTTFTMLP+GLYESVVA+FENRTG+VA RA R
Sbjct: 304 SVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANRARR 363

Query: 363 IEENTGLSPCYYYDDSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDGGDG---VGKKRKV 422
           IEENTGLSPCYYY++S+ VPRVVLHFVGE+SSVVLPRKNYFYEF DGGDG   VG+KRKV
Sbjct: 364 IEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRKRKV 423

Query: 423 GCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLNRS 477
           GCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWD+LNRS
Sbjct: 424 GCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRS 481

[Close]

BLAST of Sed0003727 vs. NCBI nr
Match: XP_023553227.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 822.4 bits (2123), Expect = 2.0e-234
Identity = 412/482 (85.48%), Postives = 436/482 (90.46%), Query Frame = 0


Query: 1   MASPVFAFLCFLLSSSSVFSSQILLLPLSHSLSSNLNNNNTHNLLKSAADRSSARHRRRR 60
           MASPVF FL   L SS VFSSQ+LLLPLS+SLSS+ + NNTHNLLKS A RSSAR   RR
Sbjct: 1   MASPVFLFLLCFLLSSPVFSSQLLLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 61  RT----HLSLPLSPGGDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKPQI 120
           RT    HLSLPLSPGGDYTLSFNLGS +  ISLYMDTGSD VWFPCSPFECILCEGKP+I
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSESQKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120

Query: 121 QPPFPQIPKQKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLYYA 180
           Q P P+I  +KSVSCSA ACSAAHG SLSASHLCAISRCPLESIEVSECSSFSCPP YYA
Sbjct: 121 QSPLPKIADKKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYYA 180

Query: 181 YGDGSLIAQLYRDSLGL--PAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPS 240
           YGDGSLI +LYRDSL L  PAPAPSP I VRNFTFGCAH+ALGEP+GVAGFGRG LSMPS
Sbjct: 181 YGDGSLIGRLYRDSLSLPAPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPS 240

Query: 241 QLATFSPQLGNRFSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDNPKHPYFY 300
           QLATFSPQLGNRFSYCLVSHSF  DRVRRPSPLILGRY   ++EF+YTSLL+NPKHPYFY
Sbjct: 241 QLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSETEFIYTSLLENPKHPYFY 300

Query: 301 SVGLAGISVGTVTIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGR 360
           SVGLAGISVG+V IPAPEFLKRVD+GGSGGVVVDSGTTFTMLPAGLY SVVAQFENRTGR
Sbjct: 301 SVGLAGISVGSVRIPAPEFLKRVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTGR 360

Query: 361 VATRASRIEENTGLSPCYYYDDSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDGGDGVGK 420
           VA+RASRIEENTGLSPCYYY++S++VPRVVLHFVGE+SSVVLPRKNYFYEF DGGDGV +
Sbjct: 361 VASRASRIEENTGLSPCYYYENSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVER 420

Query: 421 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLN 477
           KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWDSLN
Sbjct: 421 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSLN 480

[Close]

BLAST of Sed0003727 vs. NCBI nr
Match: XP_023007805.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 820.5 bits (2118), Expect = 7.7e-234
Identity = 408/480 (85.00%), Postives = 433/480 (90.21%), Query Frame = 0


Query: 1   MASPVFAFLCFLLSSSSVFSSQILLLPLSHSLSSNLNNNNTHNLLKSAADRSSARHRRRR 60
           MASPVF FL   L  S VFSSQILLLPLS+SLSS+ + NNTHNLLKS A RSSAR   RR
Sbjct: 1   MASPVFLFLLCFLLPSPVFSSQILLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 61  RT----HLSLPLSPGGDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKPQI 120
           RT    HLSLPLSPGGDYTLSFNLGS +  ISLYMDTGSD VWFPCSPFECILCEGKP+I
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSESQKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120

Query: 121 QPPFPQIPKQKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLYYA 180
           Q P P+I  QKSVSCSA ACSAAHG SLSASHLCAISRCPLESIEVSECSSFSCPP YYA
Sbjct: 121 QSPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYYA 180

Query: 181 YGDGSLIAQLYRDSLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPSQL 240
           YGDGSLI +LYRDSL LPAPAPSP I VRNFTFGCAH+ALGEP+GVAGFGRG LSMP QL
Sbjct: 181 YGDGSLIGRLYRDSLSLPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPIQL 240

Query: 241 ATFSPQLGNRFSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDNPKHPYFYSV 300
           ATFSPQLGNRFSYCLVSHSF  DRVRRPSPLILGRY   ++EF+YTS+L+NPKHPYFYSV
Sbjct: 241 ATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSETEFIYTSMLENPKHPYFYSV 300

Query: 301 GLAGISVGTVTIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGRVA 360
           GLAGISVG+V IPAPEFLK+VD+GGSGGVVVDSGTTFTMLPAGLY SVVAQFENRTGRVA
Sbjct: 301 GLAGISVGSVMIPAPEFLKKVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTGRVA 360

Query: 361 TRASRIEENTGLSPCYYYDDSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDGGDGVGKKR 420
           +RAS+IEENTGLSPCYYY+ S++VPRVVLHFVGE+SSV+LPRKNYFYEF DGGDGVG+K 
Sbjct: 361 SRASQIEENTGLSPCYYYEKSVEVPRVVLHFVGEKSSVMLPRKNYFYEFLDGGDGVGRKI 420

Query: 421 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLNRS 477
           KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWDSLNRS
Sbjct: 421 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSLNRS 480

[Close]

BLAST of Sed0003727 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.3e-164
Identity = 296/490 (60.41%), Postives = 363/490 (74.08%), Query Frame = 0


Query: 11  FLLSSSSVFSSQILLLPLSHSLSSNLNNNNTHNLLKSAADRSSAR----HRRRRRTHLSL 70
           F  S SS+  S  LLL LSHSLS++ ++++  +LLKS++ RSSAR    H ++++  LSL
Sbjct: 18  FHFSVSSL--STPLLLHLSHSLSTSKHSSSPLHLLKSSSSRSSARFRRHHHKQQQQQLSL 77

Query: 71  PLSPGGDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKP-QIQPPFPQIPK 130
           P+S G DY +S ++GS +  +SLY+DTGSD VWFPC PF CILCE KP    PP      
Sbjct: 78  PISSGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRPFTCILCESKPLPPSPPSSLSSS 137

Query: 131 QKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSEC--SSFSCPPLYYAYGDGSLI 190
             +VSCS+P+CSAAH +SL +S LCAIS CPL+ IE  +C  SS+ CPP YYAYGDGSL+
Sbjct: 138 ATTVSCSSPSCSAAH-SSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSLV 197

Query: 191 AQLYRDSLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPSQLATFSPQL 250
           A+LY DSL LP+      + V NFTFGCAHT L EP+GVAGFGRG LS+P+QLA  SP L
Sbjct: 198 AKLYSDSLSLPS------VSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHL 257

Query: 251 GNRFSYCLVSHSFNTDRVRRPSPLILGRYSD--------------------GQSEFVYTS 310
           GN FSYCLVSHSF++DRVRRPSPLILGR+ D                     ++EFV+T 
Sbjct: 258 GNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTE 317

Query: 311 LLDNPKHPYFYSVGLAGISVGTVTIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYES 370
           +L+NPKHPYFYSV L GIS+G   IPAP  L+R+D  G GGVVVDSGTTFTMLPA  Y S
Sbjct: 318 MLENPKHPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNS 377

Query: 371 VVAQFENRTGRVATRASRIEENTGLSPCYYYDDSIQVPRVVLHFVGERSSVVLPRKNYFY 430
           VV +F++R GRV  RA R+E ++G+SPCYY + +++VP +VLHF G RSSV LPR+NYFY
Sbjct: 378 VVEEFDSRVGRVHERADRVEPSSGMSPCYYLNQTVKVPALVLHFAGNRSSVTLPRRNYFY 437

Query: 431 EFFDGGDGVGKKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFAR 474
           EF DGGDG  +KRK+GCLMLMNGGDE+EL GG GA LGNYQQQGFEVVYDL   RVGFA+
Sbjct: 438 EFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGFAK 497

[Close]

BLAST of Sed0003727 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 7.2e-33
Identity = 147/485 (30.31%), Postives = 206/485 (42.47%), Query Frame = 0


Query: 14  SSSSVFSSQILLLPLSHSLSSNLNNNNTHNLLKSAADRSSARHRR----------RRRTH 73
           S S   SS  + L L H + +  +N     L  S   R S R +           R  TH
Sbjct: 62  SGSDSESSSSITLNLDH-IDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTH 121

Query: 74  LSLP----------LSPG-GDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEG 133
              P          LS G G+Y     +G+ A  + + +DTGSD VW  C+P      + 
Sbjct: 122 APRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQS 181

Query: 134 KPQIQPPFPQIPKQKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPP 193
            P   P       +KS + +   CS+ H                   ++ + C++     
Sbjct: 182 DPIFDP-------RKSKTYATIPCSSPH----------------CRRLDSAGCNTRRKTC 241

Query: 194 LY-YAYGDGSL-IAQLYRDSLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVA---GFGR 253
           LY  +YGDGS  +     ++L           RV+    GC H   G  VG A   G G+
Sbjct: 242 LYQVSYGDGSFTVGDFSTETLTFRRN------RVKGVALGCGHDNEGLFVGAAGLLGLGK 301

Query: 254 GALSMPSQLATFSPQLGNRFSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDN 313
           G LS P Q      +   +FSYCLV  S ++    +PS ++ G  +  +    +T LL N
Sbjct: 302 GKLSFPGQT---GHRFNQKFSYCLVDRSASS----KPSSVVFGNAAVSRIA-RFTPLLSN 361

Query: 314 PKHPYFYSVGLAGISVGTVTIP-APEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVA 373
           PK   FY VGL GISVG   +P     L ++D  G+GGV++DSGT+ T L    Y ++  
Sbjct: 362 PKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRD 421

Query: 374 QFENRTGRVATRASRIEENTGLSPCYYYD--DSIQVPRVVLHFVGERSSVVLPRKNYFYE 433
            F  R G  A    R  + +    C+     + ++VP VVLHF G  + V LP  NY   
Sbjct: 422 AF--RVG--AKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRG--ADVSLPATNYLIP 481

Query: 434 FFDGGDGVGKKRKVGCLMLMNGGDEAELAGGPG--ATLGNYQQQGFEVVYDLEKNRVGFA 468
                            +  NG      AG  G  + +GN QQQGF VVYDL  +RVGFA
Sbjct: 482 -----------------VDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFA 485

[Close]

BLAST of Sed0003727 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 9.4e-33
Identity = 121/402 (30.10%), Postives = 165/402 (41.04%), Query Frame = 0


Query: 72  GDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKPQIQPPFPQIPKQKSVSC 131
           G+Y     +G+ A  + L +DTGSD  W  C P      +  P   P        KS++C
Sbjct: 160 GEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNP--TSSSTYKSLTC 219

Query: 132 SAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLY-YAYGDGSL-IAQLYRD 191
           SAP CS                      +E S C S  C  LY  +YGDGS  + +L  D
Sbjct: 220 SAPQCSL---------------------LETSACRSNKC--LYQVSYGDGSFTVGELATD 279

Query: 192 SLGLPAPAPSPPIRVRNFTFGCAHTALG---EPVGVAGFGRGALSMPSQLATFSPQLGNR 251
           ++           ++ N   GC H   G      G+ G G G LS+ +Q+   S      
Sbjct: 280 TVTFGNSG-----KINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATS------ 339

Query: 252 FSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDNPKHPYFYSVGLAGISVGTV 311
           FSYCLV          + S L       G  +     LL N K   FY VGL+G SVG  
Sbjct: 340 FSYCLVDRDSG-----KSSSLDFNSVQLGGGD-ATAPLLRNKKIDTFYYVGLSGFSVGGE 399

Query: 312 TIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGRVATRASRIEENT 371
            +  P+ +  VD  GSGGV++D GT  T L    Y S+   F   T  +   +S I   +
Sbjct: 400 KVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSI---S 459

Query: 372 GLSPCYYYD--DSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDGGDGVGKKRKVGCLMLM 431
               CY +    +++VP V  HF G + S+ LP KNY     D G          C    
Sbjct: 460 LFDTCYDFSSLSTVKVPTVAFHFTGGK-SLDLPAKNYLIPVDDSG--------TFCFAFA 500

Query: 432 NGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQC 467
                  +       +GN QQQG  + YDL KN +G +  +C
Sbjct: 520 PTSSSLSI-------IGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Sed0003727 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 9.4e-33
Identity = 128/445 (28.76%), Postives = 190/445 (42.70%), Query Frame = 0


Query: 38  NNNTHNLLKSAADRSSARHRRRRRTHLSLP-------LSPGGDYTLSFNLGSHAHTISLY 97
           N     LL+ A +R S R  +R    L+ P        +  G+Y ++ ++G+ A   S  
Sbjct: 53  NLTKFQLLERAIERGS-RRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAI 112

Query: 98  MDTGSDFVWFPCSPFECILCEGKPQIQPPFPQIPKQKSVSCSAPACSAAHGASLSASHLC 157
           MDTGSD +W  C P      +  P   P       Q S S S   CS         S LC
Sbjct: 113 MDTGSDLIWTQCQPCTQCFNQSTPIFNP-------QGSSSFSTLPCS---------SQLC 172

Query: 158 AISRCPLESIEVSECSSFSCPPLYYAYGDGSLIAQLYRDSLGLPAPAPSPPIRVRNFTFG 217
                  +++    CS+  C    Y YGDGS      + S+G         + + N TFG
Sbjct: 173 -------QALSSPTCSNNFC-QYTYGYGDGSET----QGSMGTET-LTFGSVSIPNITFG 232

Query: 218 CAHT----ALGEPVGVAGFGRGALSMPSQLATFSPQLGNRFSYCLVSHSFNTDRVRRPSP 277
           C         G   G+ G GRG LS+PSQL         +FSYC+     +T     PS 
Sbjct: 233 CGENNQGFGQGNGAGLVGMGRGPLSLPSQLDV------TKFSYCMTPIGSST-----PSN 292

Query: 278 LILGRYSDG-QSEFVYTSLLDNPKHPYFYSVGLAGISVGTVTIPA-PEFLKRVDDGGSGG 337
           L+LG  ++   +    T+L+ + + P FY + L G+SVG+  +P  P       + G+GG
Sbjct: 293 LLLGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGG 352

Query: 338 VVVDSGTTFTMLPAGLYESVVAQFENRTGRVATRASRIEENTGLSPCYYY---DDSIQVP 397
           +++DSGTT T      Y+SV  +F ++        S    ++G   C+       ++Q+P
Sbjct: 353 IIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGS----SSGFDLCFQTPSDPSNLQIP 412

Query: 398 RVVLHFVGERSSVVLPRKNYFYEFFDGGDGVGKKRKVGCLMLMNGGDEAELAGGPGATLG 457
             V+HF G    + LP +NYF         +     + CL + +      +        G
Sbjct: 413 TFVMHFDG--GDLELPSENYF---------ISPSNGLICLAMGSSSQGMSI-------FG 434

Query: 458 NYQQQGFEVVYDLEKNRVGFARRQC 467
           N QQQ   VVYD   + V FA  QC
Sbjct: 473 NIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Sed0003727 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.7e-32
Identity = 128/449 (28.51%), Postives = 191/449 (42.54%), Query Frame = 0


Query: 38  NNNTHNLLKSAADRSSARHRR-----RRRTHLSLPLSPG-GDYTLSFNLGSHAHTISLYM 97
           N   + L+K A  R   R R      +  + +  P+  G G+Y ++  +G+   + S  M
Sbjct: 54  NLTKYELIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIM 113

Query: 98  DTGSDFVWFPCSPFECILCEGKPQIQPPFPQIPKQKSVSCSAPACSAAHGASLSASHLCA 157
           DTGSD +W  C P  C  C        P P    Q S S S   C + +   L       
Sbjct: 114 DTGSDLIWTQCEP--CTQC-----FSQPTPIFNPQDSSSFSTLPCESQYCQDL------- 173

Query: 158 ISRCPLESIEVSECSSFSCPPLYYAYGDGSLIAQLYRDSLGLPAPAPSPPIRVRNFTFGC 217
               P E+   +EC         Y YGDGS   Q Y  +        S P    N  FGC
Sbjct: 174 ----PSETCNNNECQ------YTYGYGDGS-TTQGYMATETFTFETSSVP----NIAFGC 233

Query: 218 AHT----ALGEPVGVAGFGRGALSMPSQLATFSPQLGNRFSYCLVSHSFNTDRVRRPSPL 277
                    G   G+ G G G LS+PSQL         +FSYC+ S+  ++     PS L
Sbjct: 234 GEDNQGFGQGNGAGLIGMGWGPLSLPSQLGV------GQFSYCMTSYGSSS-----PSTL 293

Query: 278 ILGRYSDGQSE-FVYTSLLDNPKHPYFYSVGLAGISVGTVTIPAPEFLKRVDDGGSGGVV 337
            LG  + G  E    T+L+ +  +P +Y + L GI+VG   +  P    ++ D G+GG++
Sbjct: 294 ALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMI 353

Query: 338 VDSGTTFTMLPAGLYESVVAQFENRTGRVATRASRIEENTGLSPCYYY---DDSIQVPRV 397
           +DSGTT T LP   Y +V   F ++           E ++GLS C+       ++QVP +
Sbjct: 354 IDSGTTLTYLPQDAYNAVAQAFTDQINLPTVD----ESSSGLSTCFQQPSDGSTVQVPEI 413

Query: 398 VLHF------VGERSSVVLPRKNYFYEFFDGGDGVGKKRKVGCLMLMNGGDEAELAGGPG 457
            + F      +GE++ ++ P +                  V CL +   G  ++L     
Sbjct: 414 SMQFDGGVLNLGEQNILISPAEG-----------------VICLAM---GSSSQLG---I 435

Query: 458 ATLGNYQQQGFEVVYDLEKNRVGFARRQC 467
           +  GN QQQ  +V+YDL+   V F   QC
Sbjct: 474 SIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Sed0003727 vs. ExPASy TrEMBL
Match: A0A0A0L5I7 (Pepsin A OS=Cucumis sativus OX=3659 GN=Csa_3G020060 PE=3 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 5.2e-236
Identity = 407/476 (85.50%), Postives = 433/476 (90.97%), Query Frame = 0


Query: 3   SPVFAFLCFLLSSSSVFSSQILLLPLSHSLSSNLNN-NNTHNLLKSAADRSSARHRRRRR 62
           SPVF FL   L SS VFSSQI LLPLSHSLSS++++ NNTHNLLKS A RSSAR  R R 
Sbjct: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHRHRH 63

Query: 63  THLSLPLSPGGDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKPQIQPPFP 122
            HLSLPLSPGGDYTLSFNLGS +H ISLYMDTGSD VWFPCSPFECILCEGKP+IQ P P
Sbjct: 64  NHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLP 123

Query: 123 QIPKQKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLYYAYGDGS 182
           +I   KSVSCSA ACSAAHG SLSASHLCAISRCPLESIE+SECSSFSCPP YYAYGDGS
Sbjct: 124 KIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGS 183

Query: 183 LIAQLYRDSLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPSQLATFSP 242
           L+A+LYRDSL LP PAPSPPI VRNFTFGCAHT LGEPVGVAGFGRG LSMPSQLATFSP
Sbjct: 184 LVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATFSP 243

Query: 243 QLGNRFSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDNPKHPYFYSVGLAGI 302
           QLGNRFSYCLVSHSF  DRVRRPSPLILGRY  G++EF+YTSLL+NPKHPYFYSVGLAGI
Sbjct: 244 QLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVGLAGI 303

Query: 303 SVGTVTIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGRVATRASR 362
           SVG + IPAPEFL +VD+GGSGGVVVDSGTTFTMLPAGLYESVVA+FENRTG+VA RA R
Sbjct: 304 SVGNIRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVANRARR 363

Query: 363 IEENTGLSPCYYYDDSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDGGDG-VGKKRKVGC 422
           IEENTGLSPCYYY++S+ VPRVVLHFVGE+S+VVLPRKNYFYEF DGGDG VG+KRKVGC
Sbjct: 364 IEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKRKVGC 423

Query: 423 LMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLNRS 477
           LMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWD+LNRS
Sbjct: 424 LMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRS 479

[Close]

BLAST of Sed0003727 vs. ExPASy TrEMBL
Match: A0A1S3BK28 (aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103490888 PE=3 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 4.4e-235
Identity = 406/478 (84.94%), Postives = 433/478 (90.59%), Query Frame = 0


Query: 3   SPVFAFLCFLLSSSSVFSSQILLLPLSHSLSSNLNN-NNTHNLLKSAADRSSARHRRRRR 62
           SPVF FL   L SS VFSSQI LLPLSHSLSS++++ N+THNLLKS A RSSAR  R R 
Sbjct: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHRHRH 63

Query: 63  THLSLPLSPGGDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKPQIQPPFP 122
            HLSLPLSPGGDYTLSFNLGS +H ISLYMDTGSD VWFPCSPFECILCEGKP+IQ P P
Sbjct: 64  NHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLP 123

Query: 123 QIPKQKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLYYAYGDGS 182
           +I   KSVSCSA ACSAAHG SLSASHLCAISRCPLESIE+SECSSFSCPP YYAYGDGS
Sbjct: 124 KISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGS 183

Query: 183 LIAQLYRDSLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPSQLATFSP 242
           L+A+LYRDSL LP PAPSPPI VRNFTFGCAHT LGEPVGVAGFGRG LSMPSQLATFSP
Sbjct: 184 LVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATFSP 243

Query: 243 QLGNRFSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDNPKHPYFYSVGLAGI 302
           QLGNRFSYCLVSHSF  DRVRRPSPLILGRY  G++EF+YTSLL+NPKHPYFYSVGLAGI
Sbjct: 244 QLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGLAGI 303

Query: 303 SVGTVTIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGRVATRASR 362
           SVG V IPAPEFL++VD+ GSGGVVVDSGTTFTMLP+GLYESVVA+FENRTG+VA RA R
Sbjct: 304 SVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANRARR 363

Query: 363 IEENTGLSPCYYYDDSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDGGDG---VGKKRKV 422
           IEENTGLSPCYYY++S+ VPRVVLHFVGE+SSVVLPRKNYFYEF DGGDG   VG+KRKV
Sbjct: 364 IEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRKRKV 423

Query: 423 GCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLNRS 477
           GCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWD+LNRS
Sbjct: 424 GCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRS 481

[Close]

BLAST of Sed0003727 vs. ExPASy TrEMBL
Match: A0A6J1L3Z9 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111500303 PE=3 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 3.7e-234
Identity = 408/480 (85.00%), Postives = 433/480 (90.21%), Query Frame = 0


Query: 1   MASPVFAFLCFLLSSSSVFSSQILLLPLSHSLSSNLNNNNTHNLLKSAADRSSARHRRRR 60
           MASPVF FL   L  S VFSSQILLLPLS+SLSS+ + NNTHNLLKS A RSSAR   RR
Sbjct: 1   MASPVFLFLLCFLLPSPVFSSQILLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 61  RT----HLSLPLSPGGDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKPQI 120
           RT    HLSLPLSPGGDYTLSFNLGS +  ISLYMDTGSD VWFPCSPFECILCEGKP+I
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSESQKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120

Query: 121 QPPFPQIPKQKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLYYA 180
           Q P P+I  QKSVSCSA ACSAAHG SLSASHLCAISRCPLESIEVSECSSFSCPP YYA
Sbjct: 121 QSPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYYA 180

Query: 181 YGDGSLIAQLYRDSLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPSQL 240
           YGDGSLI +LYRDSL LPAPAPSP I VRNFTFGCAH+ALGEP+GVAGFGRG LSMP QL
Sbjct: 181 YGDGSLIGRLYRDSLSLPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPIQL 240

Query: 241 ATFSPQLGNRFSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDNPKHPYFYSV 300
           ATFSPQLGNRFSYCLVSHSF  DRVRRPSPLILGRY   ++EF+YTS+L+NPKHPYFYSV
Sbjct: 241 ATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSETEFIYTSMLENPKHPYFYSV 300

Query: 301 GLAGISVGTVTIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGRVA 360
           GLAGISVG+V IPAPEFLK+VD+GGSGGVVVDSGTTFTMLPAGLY SVVAQFENRTGRVA
Sbjct: 301 GLAGISVGSVMIPAPEFLKKVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTGRVA 360

Query: 361 TRASRIEENTGLSPCYYYDDSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDGGDGVGKKR 420
           +RAS+IEENTGLSPCYYY+ S++VPRVVLHFVGE+SSV+LPRKNYFYEF DGGDGVG+K 
Sbjct: 361 SRASQIEENTGLSPCYYYEKSVEVPRVVLHFVGEKSSVMLPRKNYFYEFLDGGDGVGRKI 420

Query: 421 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLNRS 477
           KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWDSLNRS
Sbjct: 421 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSLNRS 480

[Close]

BLAST of Sed0003727 vs. ExPASy TrEMBL
Match: A0A6J1EC44 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111431201 PE=3 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 4.9e-234
Identity = 411/482 (85.27%), Postives = 434/482 (90.04%), Query Frame = 0


Query: 1   MASPVFAFLCFLLSSSSVFSSQILLLPLSHSLSSNLNNNNTHNLLKSAADRSSARHRRRR 60
           MASPVF FL   L SS VFSSQ+LLLPLS+SLSS+ + NNTHNLLKS A RSSAR   RR
Sbjct: 1   MASPVFLFLLCFLFSSPVFSSQLLLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 61  RT----HLSLPLSPGGDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKPQI 120
           RT    HLSLPLSPGGDYTLSFNLGS +  ISLYMDTGSD VWFPCSPFECILCEGKP+I
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSESQKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120

Query: 121 QPPFPQIPKQKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLYYA 180
           Q P P+I  QKSVSCSA ACSAAHG SLSASHLCAISRCPLESIEVSECSSFSCPP YYA
Sbjct: 121 QSPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYYA 180

Query: 181 YGDGSLIAQLYRDSLGL--PAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPS 240
           YGDGSLI +LYRDSL L  PAPAPSP I VRNFTFGCAH+ALGEP+GVAGFGRG LSMPS
Sbjct: 181 YGDGSLIGRLYRDSLSLPAPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPS 240

Query: 241 QLATFSPQLGNRFSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDNPKHPYFY 300
           QLATFSPQLGNRFSYCLVSHSF  DRVRRPSPLILGRY   ++EF+YTS+L+NPKHPYFY
Sbjct: 241 QLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSETEFIYTSMLENPKHPYFY 300

Query: 301 SVGLAGISVGTVTIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGR 360
           SVGLAGISVG+V IPAPEFLKRVD+GGSGGVVVDSGTTFTMLPAGLY SVVAQFENRTGR
Sbjct: 301 SVGLAGISVGSVRIPAPEFLKRVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTGR 360

Query: 361 VATRASRIEENTGLSPCYYYDDSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDGGDGVGK 420
           VA+RASRIEENTGLSPCY Y+ S++VPRVVLHFVGE+SSV LPRKNYFYEF DGGDGVG+
Sbjct: 361 VASRASRIEENTGLSPCYSYEKSVEVPRVVLHFVGEKSSVELPRKNYFYEFLDGGDGVGR 420

Query: 421 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLN 477
           KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWDSLN
Sbjct: 421 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSLN 480

[Close]

BLAST of Sed0003727 vs. ExPASy TrEMBL
Match: A0A5D3CP11 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00280 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 8.3e-234
Identity = 407/480 (84.79%), Postives = 433/480 (90.21%), Query Frame = 0


Query: 3   SPVFAFLCFLLSSSSVFSSQILLLPLSHSLSSNLNN-NNTHNLLKSAADRSSARHRRRRR 62
           SPVF FL   L SS VFSSQI LLPLSHSLSS++++ N+THNLLKS A RSSAR  R R 
Sbjct: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHRHRH 63

Query: 63  THLSLPLSPGGDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKPQIQPPFP 122
            HLSLPLSPGGDYTLSFNLGS +H ISLYMDTGSD VWFPCSPFECILCEGKP+IQ P P
Sbjct: 64  NHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLP 123

Query: 123 QIPKQKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLYYAYGDGS 182
           +I   KSVSCSA ACSAAHG SLSASHLCAISRCPLESIE+SECSSFSCPP YYAYGDGS
Sbjct: 124 KISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGDGS 183

Query: 183 LIAQLYRDSLGL--PAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPSQLATF 242
           L+A+LYRDSL L  PAPAPSPPI VRNFTFGCAHT LGEPVGVAGFGRG LSMPSQLATF
Sbjct: 184 LVARLYRDSLSLPTPAPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATF 243

Query: 243 SPQLGNRFSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDNPKHPYFYSVGLA 302
           SPQLGNRFSYCLVSHSF  DRVRRPSPLILGRY  G++EF+YTSLL+NPKHPYFYSVGLA
Sbjct: 244 SPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVGLA 303

Query: 303 GISVGTVTIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGRVATRA 362
           GISVG V IPAPEFL++VD+ GSGGVVVDSGTTFTMLP+GLYESVVA+FENRTG+VA RA
Sbjct: 304 GISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVANRA 363

Query: 363 SRIEENTGLSPCYYYDDSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDGGDG---VGKKR 422
            RIEENTGLSPCYYY +S+ VPRVVLHFVGE+SSVVLPRKNYFYEF DGGDG   VG+KR
Sbjct: 364 RRIEENTGLSPCYYYQNSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGRKR 423

Query: 423 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLNRS 477
           KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWD+LNRS
Sbjct: 424 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRS 483

[Close]

BLAST of Sed0003727 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 580.9 bits (1496), Expect = 9.6e-166
Identity = 296/490 (60.41%), Postives = 363/490 (74.08%), Query Frame = 0


Query: 11  FLLSSSSVFSSQILLLPLSHSLSSNLNNNNTHNLLKSAADRSSAR----HRRRRRTHLSL 70
           F  S SS+  S  LLL LSHSLS++ ++++  +LLKS++ RSSAR    H ++++  LSL
Sbjct: 18  FHFSVSSL--STPLLLHLSHSLSTSKHSSSPLHLLKSSSSRSSARFRRHHHKQQQQQLSL 77

Query: 71  PLSPGGDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKP-QIQPPFPQIPK 130
           P+S G DY +S ++GS +  +SLY+DTGSD VWFPC PF CILCE KP    PP      
Sbjct: 78  PISSGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRPFTCILCESKPLPPSPPSSLSSS 137

Query: 131 QKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSEC--SSFSCPPLYYAYGDGSLI 190
             +VSCS+P+CSAAH +SL +S LCAIS CPL+ IE  +C  SS+ CPP YYAYGDGSL+
Sbjct: 138 ATTVSCSSPSCSAAH-SSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSLV 197

Query: 191 AQLYRDSLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPSQLATFSPQL 250
           A+LY DSL LP+      + V NFTFGCAHT L EP+GVAGFGRG LS+P+QLA  SP L
Sbjct: 198 AKLYSDSLSLPS------VSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHL 257

Query: 251 GNRFSYCLVSHSFNTDRVRRPSPLILGRYSD--------------------GQSEFVYTS 310
           GN FSYCLVSHSF++DRVRRPSPLILGR+ D                     ++EFV+T 
Sbjct: 258 GNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTE 317

Query: 311 LLDNPKHPYFYSVGLAGISVGTVTIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYES 370
           +L+NPKHPYFYSV L GIS+G   IPAP  L+R+D  G GGVVVDSGTTFTMLPA  Y S
Sbjct: 318 MLENPKHPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNS 377

Query: 371 VVAQFENRTGRVATRASRIEENTGLSPCYYYDDSIQVPRVVLHFVGERSSVVLPRKNYFY 430
           VV +F++R GRV  RA R+E ++G+SPCYY + +++VP +VLHF G RSSV LPR+NYFY
Sbjct: 378 VVEEFDSRVGRVHERADRVEPSSGMSPCYYLNQTVKVPALVLHFAGNRSSVTLPRRNYFY 437

Query: 431 EFFDGGDGVGKKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFAR 474
           EF DGGDG  +KRK+GCLMLMNGGDE+EL GG GA LGNYQQQGFEVVYDL   RVGFA+
Sbjct: 438 EFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGFAK 497

[Close]

BLAST of Sed0003727 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.7e-50
Identity = 137/418 (32.78%), Postives = 200/418 (47.85%), Query Frame = 0


Query: 74  YTLSFNLGSHAHTISLYMDTGSDFVWFPCS--PFECILCEG--KPQIQPPFPQIPKQKSV 133
           Y ++ N+G+    + +Y+DTGSD  W PC    F+CI C       ++ P    P   S 
Sbjct: 83  YLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDCIECYDLKNNDLKSPSVFSPLHSST 142

Query: 134 ----SCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLYYAYGDGSLIAQ 193
               SC++  C   H +S +    CA++ C +  +  S C    CP   Y YG+G LI+ 
Sbjct: 143 SFRDSCASSFCVEIH-SSDNPFDPCAVAGCSVSMLLKSTCVR-PCPSFAYTYGEGGLISG 202

Query: 194 LYRDSLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVAGFGRGALSMPSQLATFSPQLGN 253
           +    + L A     P     F+FGC  +   EP+G+AGFGRG LS+PSQL      L  
Sbjct: 203 ILTRDI-LKARTRDVP----RFSFGCVTSTYREPIGIAGFGRGLLSLPSQLGF----LEK 262

Query: 254 RFSYCLVSHSFNTDRVRRPSPLILGRYS---DGQSEFVYTSLLDNPKHPYFYSVGLAGIS 313
            FS+C +   F  +     SPLILG  +   +      +T +L+ P +P  Y +GL  I+
Sbjct: 263 GFSHCFLPFKF-VNNPNISSPLILGASALSINLTDSLQFTPMLNTPMYPNSYYIGLESIT 322

Query: 314 VGTVTIP--APEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGRVATRAS 373
           +GT   P   P  L++ D  G+GG++VDSGTT+T LP   Y  ++   ++       RA+
Sbjct: 323 IGTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFYSQLLTTLQSTI--TYPRAT 382

Query: 374 RIEENTGLSPCYYY------------DDSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDG 433
             E  TG   CY              D  +  P +  HF+   ++++LP+ N FY     
Sbjct: 383 ETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFL-NNATLLLPQGNSFYAMSAP 442

Query: 434 GDGVGKKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQC 467
            DG      V CL+  N  D      GP    G++QQQ  +VVYDLEK R+GF    C
Sbjct: 443 SDG----SVVQCLLFQNMEDGDY---GPAGVFGSFQQQNVKVVYDLEKERIGFQAMDC 478

[Close]

BLAST of Sed0003727 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 176.4 bits (446), Expect = 5.4e-44
Identity = 155/507 (30.57%), Postives = 227/507 (44.77%), Query Frame = 0


Query: 1   MASPVFAFLCFLLSSSSVFSSQILLLPLSHSLSSNLNNNNTHNLLKSAADRSSARHRRRR 60
           MAS +F F  FL+  S V + ++ L P SH   S+ +  + +  L+  A+ S AR  + +
Sbjct: 1   MASSIFFF--FLIFLSVVSAVKLPLSPFSH---SDQSPKDPYLSLRRLAESSIARAHKLK 60

Query: 61  R-------------------THLSLPLSPG--GDYTLSFNLGSHAHTISLYMDTGSDFVW 120
                               T +  PLS    G Y++S + G+ + TI    DTGS  VW
Sbjct: 61  HGTSIKPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVW 120

Query: 121 FPC-SPFECILCEGK---PQIQPPFPQIPKQKS----VSCSAPACSAAHGASLSASHLCA 180
            PC S + C  C+     P + P F  IPK  S    + C +P C   +G ++       
Sbjct: 121 LPCTSRYLCSGCDFSGLDPTLIPRF--IPKNSSSSKIIGCQSPKCQFLYGPNV------- 180

Query: 181 ISRCPLESIEVSECSSFSCPPLYYAYGDGSLIAQLYRDSLGLPAPAPSPPIRVRNFTFGC 240
             +C         C +  CPP    YG GS    L  + L        P + V +F  GC
Sbjct: 181 --QCRGCDPNTRNC-TVGCPPYILQYGLGSTAGVLITEKLDF------PDLTVPDFVVGC 240

Query: 241 AHTALGEPVGVAGFGRGALSMPSQLATFSPQLGNRFSYCLVSHSFNTDRVRRPSPLILGR 300
           +  +  +P G+AGFGRG +S+PSQ+         RFS+CLVS  F+   V     L  G 
Sbjct: 241 SIISTRQPAGIAGFGRGPVSLPSQMNL------KRFSHCLVSRRFDDTNVTTDLDLDTGS 300

Query: 301 YSDGQSE---FVYTSLLDNPK-----HPYFYSVGLAGISVGTVTIPAPEFLKRVDDGGSG 360
             +  S+     YT    NP         +Y + L  I VG   +  P         G G
Sbjct: 301 GHNSGSKTPGLTYTPFRKNPNVSNKAFLEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDG 360

Query: 361 GVVVDSGTTFTMLPAGLYESVVAQFENRTGRVATRASRIEENTGLSPCYYYD--DSIQVP 420
           G +VDSG+TFT +   ++E V  +F ++     TR   +E+ TGL PC+       + VP
Sbjct: 361 GSIVDSGSTFTFMERPVFELVAEEFASQMSNY-TREKDLEKETGLGPCFNISGKGDVTVP 420

Query: 421 RVVLHFVGERSSVVLPRKNYFYEFFDGGDGVGKKRKVGCLMLMNGGDEAELAG-GPGATL 468
            ++  F G  + + LP  NYF  F    D V       CL +++        G GP   L
Sbjct: 421 ELIFEFKG-GAKLELPLSNYF-TFVGNTDTV-------CLTVVSDKTVNPSGGTGPAIIL 468

[Close]

BLAST of Sed0003727 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 144.4 bits (363), Expect = 2.3e-34
Identity = 122/402 (30.35%), Postives = 176/402 (43.78%), Query Frame = 0


Query: 72  GDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEGKPQIQPPFPQIPKQKSVSC 131
           G+Y     +G  A  + + +DTGSD  W  C+P      + +P  +P        + +SC
Sbjct: 146 GEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQTEPIFEP--SSSSSYEPLSC 205

Query: 132 SAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPPLY-YAYGDGS-LIAQLYRD 191
             P C+A                     +EVSEC + +C  LY  +YGDGS  +     +
Sbjct: 206 DTPQCNA---------------------LEVSECRNATC--LYEVSYGDGSYTVGDFATE 265

Query: 192 SLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVA---GFGRGALSMPSQLATFSPQLGNR 251
           +L + +        V+N   GC H+  G  VG A   G G G L++PSQL T S      
Sbjct: 266 TLTIGSTL------VQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTS------ 325

Query: 252 FSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDNPKHPYFYSVGLAGISVGTV 311
           FSYCLV    ++      S +  G  +    + V   LL N +   FY +GL GISVG  
Sbjct: 326 FSYCLVDRDSDS-----ASTVDFG--TSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGE 385

Query: 312 TIPAPEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVAQFENRTGRVATRASRIEENT 371
            +  P+    +D+ GSGG+++DSGT  T L   +Y S+   F   T  +   A     +T
Sbjct: 386 LLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDT 445

Query: 372 GLSPCYYYD--DSIQVPRVVLHFVGERSSVVLPRKNYFYEFFDGGDGVGKKRKVGCLMLM 431
               CY      +++VP V  HF G +  + LP KNY        D VG      CL   
Sbjct: 446 ----CYNLSAKTTVEVPTVAFHFPGGK-MLALPAKNYMIPV----DSVG----TFCLAFA 483

Query: 432 NGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQC 467
                        A +GN QQQG  V +DL  + +GF+  +C
Sbjct: 506 PTASSL-------AIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

[Close]

BLAST of Sed0003727 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 143.3 bits (360), Expect = 5.1e-34
Identity = 147/485 (30.31%), Postives = 206/485 (42.47%), Query Frame = 0


Query: 14  SSSSVFSSQILLLPLSHSLSSNLNNNNTHNLLKSAADRSSARHRR----------RRRTH 73
           S S   SS  + L L H + +  +N     L  S   R S R +           R  TH
Sbjct: 62  SGSDSESSSSITLNLDH-IDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTH 121

Query: 74  LSLP----------LSPG-GDYTLSFNLGSHAHTISLYMDTGSDFVWFPCSPFECILCEG 133
              P          LS G G+Y     +G+ A  + + +DTGSD VW  C+P      + 
Sbjct: 122 APRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQS 181

Query: 134 KPQIQPPFPQIPKQKSVSCSAPACSAAHGASLSASHLCAISRCPLESIEVSECSSFSCPP 193
            P   P       +KS + +   CS+ H                   ++ + C++     
Sbjct: 182 DPIFDP-------RKSKTYATIPCSSPH----------------CRRLDSAGCNTRRKTC 241

Query: 194 LY-YAYGDGSL-IAQLYRDSLGLPAPAPSPPIRVRNFTFGCAHTALGEPVGVA---GFGR 253
           LY  +YGDGS  +     ++L           RV+    GC H   G  VG A   G G+
Sbjct: 242 LYQVSYGDGSFTVGDFSTETLTFRRN------RVKGVALGCGHDNEGLFVGAAGLLGLGK 301

Query: 254 GALSMPSQLATFSPQLGNRFSYCLVSHSFNTDRVRRPSPLILGRYSDGQSEFVYTSLLDN 313
           G LS P Q      +   +FSYCLV  S ++    +PS ++ G  +  +    +T LL N
Sbjct: 302 GKLSFPGQT---GHRFNQKFSYCLVDRSASS----KPSSVVFGNAAVSRIA-RFTPLLSN 361

Query: 314 PKHPYFYSVGLAGISVGTVTIP-APEFLKRVDDGGSGGVVVDSGTTFTMLPAGLYESVVA 373
           PK   FY VGL GISVG   +P     L ++D  G+GGV++DSGT+ T L    Y ++  
Sbjct: 362 PKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRD 421

Query: 374 QFENRTGRVATRASRIEENTGLSPCYYYD--DSIQVPRVVLHFVGERSSVVLPRKNYFYE 433
            F  R G  A    R  + +    C+     + ++VP VVLHF G  + V LP  NY   
Sbjct: 422 AF--RVG--AKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRG--ADVSLPATNYLIP 481

Query: 434 FFDGGDGVGKKRKVGCLMLMNGGDEAELAGGPG--ATLGNYQQQGFEVVYDLEKNRVGFA 468
                            +  NG      AG  G  + +GN QQQGF VVYDL  +RVGFA
Sbjct: 482 -----------------VDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFA 485

The following BLAST results are available for this feature:

BLAST of Sed0003727 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147205.1	1.1e-235	85.50	probable aspartyl protease At4g16563 [Cucumis sativus]	[more]
XP_038905814.1	2.4e-235	84.82	probable aspartyl protease At4g16563 [Benincasa hispida]	[more]
XP_008448851.1	9.1e-235	84.94	PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo]	[more]
XP_023553227.1	2.0e-234	85.48	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]
XP_023007805.1	7.7e-234	85.00	probable aspartyl protease At4g16563 [Cucurbita maxima]	[more]

BLAST of Sed0003727 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	1.3e-164	60.41	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q9LNJ3	7.2e-33	30.31	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LS40	9.4e-33	30.10	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q766C3	9.4e-33	28.76	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	2.7e-32	28.51	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]

BLAST of Sed0003727 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5I7	5.2e-236	85.50	Pepsin A OS=Cucumis sativus OX=3659 GN=Csa_3G020060 PE=3 SV=1	[more]
A0A1S3BK28	4.4e-235	84.94	aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103490888 PE=3 S...	[more]
A0A6J1L3Z9	3.7e-234	85.00	probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111500303...	[more]
A0A6J1EC44	4.9e-234	85.27	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114312...	[more]
A0A5D3CP11	8.3e-234	84.79	Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

BLAST of Sed0003727 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16563.1	9.6e-166	60.41	Eukaryotic aspartyl protease family protein	[more]
AT5G45120.1	1.7e-50	32.78	Eukaryotic aspartyl protease family protein	[more]
AT3G52500.1	5.4e-44	30.57	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	2.3e-34	30.35	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	5.1e-34	30.31	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 268..472 e-value: 5.5E-47 score: 161.8
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 51..260 e-value: 2.4E-33 score: 117.7
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 69..471
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 293..462 e-value: 2.1E-26 score: 92.6
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 74..269 e-value: 6.4E-28 score: 98.2
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 1..471
None	No IPR available	PANTHER	PTHR47967:SF26	BNAA01G17170D PROTEIN	coord: 1..471
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 325..336
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 74..462 score: 30.163183
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 74..466 e-value: 1.05257E-68 score: 218.671

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0003727.1	Sed0003727.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004190	aspartic-type endopeptidase activity