Sed0003445 (gene) Chayote v1

BLAST of Sed0003445 vs. NCBI nr
Match: XP_008438477.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo] >KAA0049224.1 protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa] >TYK17334.1 protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 973.0 bits (2514), Expect = 1.2e-279
Identity = 486/594 (81.82%), Postives = 547/594 (92.09%), Query Frame = 0

Query: 1   MATTNSVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTN 60
           MA  ++ EE+G +DG+TKDGTVDLKGNPVLRS  GRWKACSFIVVYE+FERMAYYGISTN
Sbjct: 1   MAAASAAEETG-LDGYTKDGTVDLKGNPVLRSQRGRWKACSFIVVYEVFERMAYYGISTN 60

Query: 61  LIIYLTTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMG 120
           LIIYLT KLHQGTVTSANNVTNW+GTVW+MP+LGAY+ADA+LGRYRTFL+AS ICLTGMG
Sbjct: 61  LIIYLTKKLHQGTVTSANNVTNWSGTVWIMPILGAYVADAHLGRYRTFLIASAICLTGMG 120

Query: 121 LLTLAVSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQ 180
           +LTLAVS+PSLKPP CL+VNK+NC++AS LQL VFFGALY LALGTGGTKPNISTMGADQ
Sbjct: 121 ILTLAVSLPSLKPPPCLDVNKENCEKASTLQLVVFFGALYLLALGTGGTKPNISTMGADQ 180

Query: 181 FDEYDPKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILI 240
           FDE+DPKEKAQKLSFFNWWMFSI+FGTLF++TILVYIQDNVGWSLGYGLPTIGLA+SI+I
Sbjct: 181 FDEFDPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISIVI 240

Query: 241 FVAGTPFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRID 300
           F+AGTPFYRHK+P+GSPFT M +VIV AIRNW +P+P+DPK+LHEL+F DY+KQGT RID
Sbjct: 241 FLAGTPFYRHKIPNGSPFTTMVSVIVGAIRNWRIPVPNDPKELHELEFEDYSKQGTFRID 300

Query: 301 STSSLRFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLF 360
           STSS RFLNKAAI    + G+ KLC+VTQVEETKQ+L+MIPIL+CTF+PSTMLAQTHTLF
Sbjct: 301 STSSFRFLNKAAI----RRGSWKLCTVTQVEETKQMLKMIPILICTFIPSTMLAQTHTLF 360

Query: 361 IKQGTTLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQ 420
           IKQGTTLDRSVG+  FKIP ASL +FVTISML+SV IYDR+FVKIMQ+ TKNPRGITLLQ
Sbjct: 361 IKQGTTLDRSVGS-HFKIPAASLASFVTISMLLSVVIYDRVFVKIMQRITKNPRGITLLQ 420

Query: 421 RMGIGMILHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADA 480
           RMGIGMILHI+IMTIASR+E HRL+VAR     +NG  + LPLTIFTLLPQFMLMGTADA
Sbjct: 421 RMGIGMILHILIMTIASRVETHRLKVAR-----ENGSPQVLPLTIFTLLPQFMLMGTADA 480

Query: 481 FIEVAKIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNL 540
           F+EVAKIEFFYDQAPETMKSLGTS+SMTSLGIGNFLS+FLLSTVS IT +NGN W+LNNL
Sbjct: 481 FMEVAKIEFFYDQAPETMKSLGTSFSMTSLGIGNFLSTFLLSTVSHITIKNGNGWVLNNL 540

Query: 541 NASHLDYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQVIADELKDKKFMG 595
           N+SHLDYYYALIAVLS INF VFL+VS+FYVYKAEVS SI+V+ADELKDKKF G
Sbjct: 541 NSSHLDYYYALIAVLSTINFFVFLIVSKFYVYKAEVSTSIKVLADELKDKKFKG 583

BLAST of Sed0003445 vs. NCBI nr
Match: XP_038875745.1 (protein NRT1/ PTR FAMILY 5.2-like [Benincasa hispida])

HSP 1 Score: 971.8 bits (2511), Expect = 2.6e-279
Identity = 489/590 (82.88%), Postives = 543/590 (92.03%), Query Frame = 0

Query: 5   NSVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIY 64
           ++ EE+G +D +TKDGTVDLKGNP+LRS  GRWKACSFIVVYEMFERMAYYGISTNLIIY
Sbjct: 4   SAAEETG-LDDYTKDGTVDLKGNPILRSRRGRWKACSFIVVYEMFERMAYYGISTNLIIY 63

Query: 65  LTTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTL 124
           LT KL+QGTVTSANNVTNW+GTVW+MP+LGAY+ADA+LGRY TFLVAS ICLTGMG+LTL
Sbjct: 64  LTKKLNQGTVTSANNVTNWSGTVWIMPILGAYVADAHLGRYWTFLVASAICLTGMGILTL 123

Query: 125 AVSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEY 184
           AVS+PSLKPP+CL+VNK+ C+QAS LQLAVFFGALY LA GTGGTKPNISTMGADQFDE+
Sbjct: 124 AVSLPSLKPPTCLDVNKEKCKQASTLQLAVFFGALYMLAFGTGGTKPNISTMGADQFDEF 183

Query: 185 DPKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAG 244
            PKEKAQKLSFFNWWMFSI+FGTLF++TILVYIQDNVGWSLGYGLPTIGLA+SILIF+AG
Sbjct: 184 HPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFLAG 243

Query: 245 TPFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSS 304
           TPFYRHK+P+GSPFT M +VIVAA+RNW +PLP+DPKQLHELDF +YTKQGT RIDSTSS
Sbjct: 244 TPFYRHKIPNGSPFTTMVSVIVAALRNWRIPLPNDPKQLHELDFEEYTKQGTFRIDSTSS 303

Query: 305 LRFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQG 364
           LRFLNKAAI+  S + + KLC+VTQVEETKQILRMIPIL+CTF+PSTMLAQTHTLFIKQG
Sbjct: 304 LRFLNKAAIRRGSSDRSWKLCTVTQVEETKQILRMIPILICTFIPSTMLAQTHTLFIKQG 363

Query: 365 TTLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGI 424
           TTLDRSVG+  F+IPPASL +FVTISML SV IYDR+FVKIMQ+ TKNPRGITLLQRMGI
Sbjct: 364 TTLDRSVGS-HFQIPPASLASFVTISMLFSVVIYDRLFVKIMQRITKNPRGITLLQRMGI 423

Query: 425 GMILHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEV 484
           GMILHI+IMTIASR+E HRL+VAR     +NG  + LPLTIFTLLPQFMLMGTADAF+EV
Sbjct: 424 GMILHILIMTIASRVESHRLKVAR-----ENGSPQVLPLTIFTLLPQFMLMGTADAFMEV 483

Query: 485 AKIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNLNASH 544
           AKIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVS IT +NGN WILNNLN+SH
Sbjct: 484 AKIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITIKNGNGWILNNLNSSH 543

Query: 545 LDYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQVIADELKDKKFMG 595
           LDYYYALIAVLS INF VFL +S+FYVYKAEVS SI+V+ADELKDKK  G
Sbjct: 544 LDYYYALIAVLSTINFFVFLFISKFYVYKAEVSTSIKVLADELKDKKTKG 586

BLAST of Sed0003445 vs. NCBI nr
Match: XP_022979366.1 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 971.5 bits (2510), Expect = 3.4e-279
Identity = 486/591 (82.23%), Postives = 542/591 (91.71%), Query Frame = 0

Query: 5   NSVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIY 64
           ++ EESG VD +TKDGTVDLKGNPVLRS  GRWKACSFIVVYE+FERMAYYGISTNLII+
Sbjct: 4   SAAEESG-VDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNLIIF 63

Query: 65  LTTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTL 124
           LT KLHQGTV SANNVTNW+GTVW+MP+LGAYIADA+LGRYRTFL+AS ICLTGMGLLTL
Sbjct: 64  LTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGLLTL 123

Query: 125 AVSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEY 184
           AVS+PSLKPP CL+VNK NC+ AS LQLAVFFGALY LALGTGGTKPNIST+GADQFDE+
Sbjct: 124 AVSLPSLKPPPCLDVNKGNCKPASTLQLAVFFGALYMLALGTGGTKPNISTIGADQFDEF 183

Query: 185 DPKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAG 244
            PKEKAQKLSFFNWWMFSI+FGTLF++TILVYIQDNVGWSLGYGLPTIGLA+SILIFVAG
Sbjct: 184 HPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAG 243

Query: 245 TPFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSS 304
           TPFYRHKLP+GSPFTKMA+VIVAA+RNW LPLP+DPK+LHEL F +Y KQG  RIDST S
Sbjct: 244 TPFYRHKLPTGSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFRIDSTPS 303

Query: 305 LRFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQG 364
           LRFLNKAAI+  S + + KLC+VTQVEETKQ+LRMIP+L+CTF+PSTMLAQTHTLFIKQG
Sbjct: 304 LRFLNKAAIRRGSSD-SWKLCTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTLFIKQG 363

Query: 365 TTLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGI 424
           TTLDRS+G+  F+IPPASL AFVTISML+SV IYDR+FVKIMQ+ TKNPRGITLLQRMGI
Sbjct: 364 TTLDRSIGS-HFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQRMGI 423

Query: 425 GMILHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEV 484
           GMILH++IM IASR+ERHRL+VAR NG V NG  + LPLTIFTLLPQFML+G ADAF EV
Sbjct: 424 GMILHVLIMIIASRVERHRLDVARQNGQVGNGSGQALPLTIFTLLPQFMLVGVADAFTEV 483

Query: 485 AKIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNLNASH 544
           AKIEFFYDQAPE+MKSLGTSYSMTS+GIGNFLSSFLLSTVS IT ++GN WI+NNLNASH
Sbjct: 484 AKIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKHGNGWIMNNLNASH 543

Query: 545 LDYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQVIADELKDKKFMGF 596
           LDYYYA +AVLSAINF +FLL+S+FYVYKAEVSGSI+ +ADELKDKK   F
Sbjct: 544 LDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKALADELKDKKLKPF 591

BLAST of Sed0003445 vs. NCBI nr
Match: XP_022924429.1 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 970.3 bits (2507), Expect = 7.6e-279
Identity = 486/591 (82.23%), Postives = 541/591 (91.54%), Query Frame = 0

Query: 5   NSVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIY 64
           ++ EESG VD +TKDGTVDLKGNPVLRS  GRWKACSFIVVYE+FERMAYYGISTNLII+
Sbjct: 4   SAAEESG-VDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNLIIF 63

Query: 65  LTTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTL 124
           LT KLHQGTV SANNVTNW+GTVW+MP+LGAYIADA+LGRYRTFL+AS ICLTGMGLLTL
Sbjct: 64  LTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGLLTL 123

Query: 125 AVSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEY 184
           AVS+PSLKPP CL+VNK NC+ AS LQLAVFFGALY LALGTGGTKPNIST+GADQFDE+
Sbjct: 124 AVSLPSLKPPPCLDVNKGNCKPASTLQLAVFFGALYMLALGTGGTKPNISTIGADQFDEF 183

Query: 185 DPKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAG 244
            PKEKAQKLSFFNWWMFSI+FGTLF++TILVYIQDNVGWSLGYGLPTIGLA+SILIFVAG
Sbjct: 184 HPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAG 243

Query: 245 TPFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSS 304
           TPFYRHKLP+GSPFTKMA+VIVAA+RNW LPLP+DPK+LHEL F +Y KQG  RIDST S
Sbjct: 244 TPFYRHKLPTGSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFRIDSTPS 303

Query: 305 LRFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQG 364
           LRFLNKAAI+  S + + KLC+VTQVEETKQ+LRMIP+L+CTF+PSTMLAQTHTLFIKQG
Sbjct: 304 LRFLNKAAIRRGSSD-SWKLCTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTLFIKQG 363

Query: 365 TTLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGI 424
           TTLDRS+G+  F+IPPASL AFVTISML+SV IYDR+FVKIMQ+ TKNPRGITLLQRMGI
Sbjct: 364 TTLDRSIGS-HFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQRMGI 423

Query: 425 GMILHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEV 484
           GMILH++IM IASR+ERHRL+VAR NG V N   + LPLTIFTLLPQFML+G ADAF EV
Sbjct: 424 GMILHVLIMIIASRVERHRLDVARQNGQVGNASGQALPLTIFTLLPQFMLVGVADAFTEV 483

Query: 485 AKIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNLNASH 544
           AKIEFFYDQAPE+MKSLGTSYSMTS+GIGNFLSSFLLSTVS IT + GN WI+NNLNASH
Sbjct: 484 AKIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKRGNGWIMNNLNASH 543

Query: 545 LDYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQVIADELKDKKFMGF 596
           LDYYYA +AVLSAINF +FLL+S+FYVYKAEVSGSI+V+ADELKDKK   F
Sbjct: 544 LDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKVLADELKDKKLKPF 591

BLAST of Sed0003445 vs. NCBI nr
Match: KAG6597346.1 (Protein NRT1/ PTR FAMILY 5.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 961.1 bits (2483), Expect = 4.6e-276
Identity = 489/585 (83.59%), Postives = 540/585 (92.31%), Query Frame = 0

Query: 6   SVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIYL 65
           S++E+G +DG+T+DGTVDL GNPVLRS  GRWKACSFIVVYE+FERMAY+GISTNLI+Y+
Sbjct: 4   SLQETG-LDGYTEDGTVDLHGNPVLRSKTGRWKACSFIVVYEVFERMAYHGISTNLILYM 63

Query: 66  TTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTLA 125
           T KLHQGTV SANNVTNW+GTVW+MP+LGAYIADA+LGRYRTF++AS ICL GMGLLTLA
Sbjct: 64  TRKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFMIASTICLMGMGLLTLA 123

Query: 126 VSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEYD 185
           VS+ SLKPP CLEVNK NC+QASKLQLAVFFGALY LALGTGGTKPNIST+GADQFD++ 
Sbjct: 124 VSISSLKPPPCLEVNKGNCKQASKLQLAVFFGALYILALGTGGTKPNISTIGADQFDDFH 183

Query: 186 PKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAGT 245
           PKEKAQKLSFFNWWMFSI+FGTLF++T +VYIQDNVGWSLGYGLPTIGLAVSILIFVAGT
Sbjct: 184 PKEKAQKLSFFNWWMFSIFFGTLFANTGVVYIQDNVGWSLGYGLPTIGLAVSILIFVAGT 243

Query: 246 PFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSSL 305
           PFYRHKLP GSPFT+MANVIVAA RNW LPLP+DPK+LHEL+F  Y+ +   RIDSTSSL
Sbjct: 244 PFYRHKLPGGSPFTRMANVIVAAARNWRLPLPNDPKELHELEFEKYSIERAFRIDSTSSL 303

Query: 306 RFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQGT 365
           RFLNKAAI+T S E   KLCSVTQVEETKQIL+MIPILVCTF+PSTMLAQTHTLFIKQGT
Sbjct: 304 RFLNKAAIRTGSTE-PWKLCSVTQVEETKQILKMIPILVCTFIPSTMLAQTHTLFIKQGT 363

Query: 366 TLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGIG 425
           TLDRSVG+  F+IPPASL+AFVTISML+SV IYDRIFVKIMQ+ TKNPRGITLLQRMGIG
Sbjct: 364 TLDRSVGS-HFQIPPASLSAFVTISMLLSVVIYDRIFVKIMQRLTKNPRGITLLQRMGIG 423

Query: 426 MILHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEVA 485
           MILHIMIMTIASR+ER RL VAR NGLVQNG   ++PLTIFTLLPQF+LMGTADAFIEVA
Sbjct: 424 MILHIMIMTIASRVERRRLYVARENGLVQNG--GEIPLTIFTLLPQFILMGTADAFIEVA 483

Query: 486 KIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNLNASHL 545
           KIEFFYDQAPE+MKSLGTSY+MTSLG+GNFLSSFLLSTVS ITK NGN WILNNLNASHL
Sbjct: 484 KIEFFYDQAPESMKSLGTSYAMTSLGVGNFLSSFLLSTVSHITKNNGNGWILNNLNASHL 543

Query: 546 DYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQVIADELKDK 591
           DYYYALIAVLSAINF VFL+VS+ YVYKAEV GSI V+ADELK++
Sbjct: 544 DYYYALIAVLSAINFFVFLIVSKLYVYKAEVCGSINVLADELKER 583

BLAST of Sed0003445 vs. ExPASy Swiss-Prot
Match: Q9FNL7 (Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 1.9e-227
Identity = 395/585 (67.52%), Postives = 481/585 (82.22%), Query Frame = 0

Query: 6   SVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIYL 65
           +VEE G  D +TKDGTVDL+GNPV RS  GRWKACSF+VVYE+FERMAYYGIS+NL IY+
Sbjct: 2   TVEEVG--DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYM 61

Query: 66  TTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTLA 125
           TTKLHQGTV S+NNVTNW GT W+ P+LGAY+ DA LGRY TF+++  I  +GM +LTL+
Sbjct: 62  TTKLHQGTVKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLS 121

Query: 126 VSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEYD 185
           V++P +KPP C   N +NC++AS LQLAVFFGALYTLA+GTGGTKPNIST+GADQFD +D
Sbjct: 122 VTIPGIKPPECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFD 181

Query: 186 PKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAGT 245
           PKEK QKLSFFNWWMFSI+FGTLF++T+LVY+QDNVGW+LGYGLPT+GLA+SI IF+ GT
Sbjct: 182 PKEKTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGT 241

Query: 246 PFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSSL 305
           PFYRHKLP+GSPFTKMA VIVA+ R  N P+  D    HEL   +Y ++G   I  T SL
Sbjct: 242 PFYRHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTPSL 301

Query: 306 RFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQGT 365
           RFL++A++KT +      LC+ T+VEETKQ+LRM+P+L  TF+PS MLAQ +TLF+KQGT
Sbjct: 302 RFLDRASLKTGTNH-KWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQGT 361

Query: 366 TLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGIG 425
           TLDR V  G F IPPASL+ FVT+SMLIS+ +YDR+FVKI +KFT NPRGITLLQRMGIG
Sbjct: 362 TLDRKV-TGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIG 421

Query: 426 MILHIMIMTIASRIERHRLEVARTNGLV-QNGEHEQLPLTIFTLLPQFMLMGTADAFIEV 485
           +I HI+IM +AS  ER+RL+VA  +GL+ Q G   +LPLTIF LLPQF+LMG AD+F+EV
Sbjct: 422 LIFHILIMIVASVTERYRLKVAADHGLIHQTG--VKLPLTIFALLPQFVLMGMADSFLEV 481

Query: 486 AKIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNLNASH 545
           AK+EFFYDQAPE+MKSLGTSYS TSL IGNF+SSFLLSTVS ITK+ G  WILNNLN S 
Sbjct: 482 AKLEFFYDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNLNESR 541

Query: 546 LDYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQVIADELKD 590
           LDYYY   AVL+ +NF++FL+V +FYVY+AEV+ S+ V   E+K+
Sbjct: 542 LDYYYLFFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMKE 580

BLAST of Sed0003445 vs. ExPASy Swiss-Prot
Match: Q9FNL8 (Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 3.3e-216
Identity = 380/577 (65.86%), Postives = 464/577 (80.42%), Query Frame = 0

Query: 6   SVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIYL 65
           +VEE G  D +TKDGTVDL+GN V RS  GRWKACSF+VVYE+FERMAYYGIS+NL+IY+
Sbjct: 2   TVEEVG--DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYM 61

Query: 66  TTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTLA 125
           TTKLHQGTV S+NNVTNW GT W+ P+LGAY+ADA+ GRY TF+++S I L GM LLTL+
Sbjct: 62  TTKLHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLS 121

Query: 126 VSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEYD 185
           VS+P LKPP C   N +NC++AS +QLAVFFGALYTLA+GTGGTKPNIST+GADQFDE+D
Sbjct: 122 VSLPGLKPPKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFD 181

Query: 186 PKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAGT 245
           PK+K  K SFFNWWMFSI+FGT F++T+LVY+QDNVGW++GYGL T+GLA SI IF+ GT
Sbjct: 182 PKDKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGT 241

Query: 246 PFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSSL 305
             YRHKLP GSPFTKMA VIVA++R    P+ SD  + +EL   +Y  +    I STSSL
Sbjct: 242 RLYRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTSSL 301

Query: 306 RFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQGT 365
           RFLN+A++KT S     +LC++T+VEETKQ+L+M+P+L  TF+PS MLAQ  TLFIKQGT
Sbjct: 302 RFLNRASLKTGSTH-KWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQGT 361

Query: 366 TLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGIG 425
           TLDR + N  F IPPASL  F T SML+S+ IYDR+FVK M+K T NPRGITLLQRMGIG
Sbjct: 362 TLDRRLTN-NFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIG 421

Query: 426 MILHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEVA 485
           MILHI+IM IAS  ER+RL+VA  +GL        +PL+IFTLLPQ++LMG ADAFIE+A
Sbjct: 422 MILHILIMIIASITERYRLKVAAEHGLTHQ-TAVPIPLSIFTLLPQYVLMGLADAFIEIA 481

Query: 486 KIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNLNASHL 545
           K+EFFYDQAPE+MKSLGTSY+ TS+ +G F+SS LLS+VS+ITK+ G  WI NNLN S L
Sbjct: 482 KLEFFYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNLNESRL 541

Query: 546 DYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQV 583
           D YY   AVL+ +NFI+FL+V RFY Y+A+V+ S  V
Sbjct: 542 DNYYMFFAVLNLLNFILFLVVIRFYEYRADVTQSANV 573

BLAST of Sed0003445 vs. ExPASy Swiss-Prot
Match: Q8VZR7 (Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2)

HSP 1 Score: 565.5 bits (1456), Expect = 7.4e-160
Identity = 290/562 (51.60%), Postives = 400/562 (71.17%), Query Frame = 0

Query: 16  HTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIYLTTKLHQGTVT 75
           +T+DGTVDL+G PVL S  GRW+ACSF++ YE FERMA+YGI++NL+ YLT +LH+ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 76  SANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTLAVSVPSLKPPS 135
           S  NV NW+G VW+ P+ GAYIAD+Y+GR+ TF  +S I + GM LLT+AV+V SL+ P+
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLR-PT 126

Query: 136 CLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEYDPKEKAQKLSF 195
           C       C +AS LQ+  F+ +LYT+A+G GGTKPNIST GADQFD Y  +EK QK+SF
Sbjct: 127 C---ENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVSF 186

Query: 196 FNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAGTPFYRHKLPSG 255
           FNWWMFS + G LF++  LVYIQ+N+GW LGYG+PT+GL VS+++F  GTPFYRHK+   
Sbjct: 187 FNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIKT 246

Query: 256 SPFTK-MANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSSLRFLNKAAIK 315
               K +  V +AA +N  L  P D  +L+ELD H Y   G  ++  T   RFL+KAAIK
Sbjct: 247 DNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAIK 306

Query: 316 TESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQGTTLDRSVGNG 375
           T S+      C+VT+VE  K++L +I I + T +PST+ AQ +TLF+KQGTTLDR +G+ 
Sbjct: 307 TSSRVP----CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRKIGS- 366

Query: 376 GFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGIGMILHIMIMT 435
            F+IP ASL +FVT+SML+SV +YD+ FV  M+K T NPRGITLLQR+G+G  + I+ + 
Sbjct: 367 NFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIVAIA 426

Query: 436 IASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEVAKIEFFYDQA 495
           IAS +E  R+ V +   +      + +P++IF LLPQ+ L+G  D F  +  +EFFYDQ+
Sbjct: 427 IASAVEVKRMRVIKEFHI--TSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQS 486

Query: 496 PETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRIT-KRNGNEWILNNLNASHLDYYYALIA 555
           PE M+SLGT++  + +G+GNFL+SFL++ + +IT K  G  WI NNLN S LDYYY  + 
Sbjct: 487 PEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGFLV 546

Query: 556 VLSAINFIVFLLVSRFYVYKAE 576
           V+S +N  +F+  +  YVYK++
Sbjct: 547 VISIVNMGLFVWAASKYVYKSD 557

BLAST of Sed0003445 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.7e-140
Identity = 262/570 (45.96%), Postives = 387/570 (67.89%), Query Frame = 0

Query: 14  DGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIYLTTKLHQGT 73
           D +T+DGTVD+  NP  +   G WKAC FI+  E  ER+AYYG+ TNL+ YL ++L+QG 
Sbjct: 5   DVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 64

Query: 74  VTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTLAVSVPSLKP 133
            T+ANNVTNW+GT ++ P++GA+IADAYLGRY T      I ++GM LLTL+ SVP LKP
Sbjct: 65  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 124

Query: 134 PSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEYDPKEKAQKL 193
            +C   N D C   S  Q AVFF ALY +ALGTGG KP +S+ GADQFDE D  EK +K 
Sbjct: 125 GNC---NADTCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 184

Query: 194 SFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAGTPFYRHKLP 253
           SFFNW+ FSI  G L ++T+LV+IQ NVGW  G+G+PT+ + +++  F  G+ FYR + P
Sbjct: 185 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 244

Query: 254 SGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSSLRFLNKAAI 313
            GSP T++  VIVAA R  ++ +P D   L E    +   +G+ ++  T +L+F +KAA+
Sbjct: 245 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV 304

Query: 314 KTES---KEG---AGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQGTTL 373
           +++S   K+G     +LCSVTQVEE K I+ ++P+     + +T+ +Q  T+F+ QG T+
Sbjct: 305 ESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTM 364

Query: 374 DRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGIGMI 433
           D+ +G   F+IP ASL+ F T+S+L    +YD+  + + +KFT+N RG T LQRMGIG++
Sbjct: 365 DQHMGK-NFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 424

Query: 434 LHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEVAKI 493
           + I  M  A  +E  RL+  +T+      + +Q+ ++IF  +PQ++L+G A+ F  + ++
Sbjct: 425 VSIFAMITAGVLEVVRLDYVKTHNAY---DQKQIHMSIFWQIPQYLLIGCAEVFTFIGQL 484

Query: 494 EFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNE-WILNNLNASHLD 553
           EFFYDQAP+ M+SL ++ S+T++ +GN+LS+ L++ V +ITK+NG   WI +NLN  HLD
Sbjct: 485 EFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLD 544

Query: 554 YYYALIAVLSAINFIVFLLVSRFYVYKAEV 577
           Y++ L+A LS +NF+V+L +S+ Y YK  V
Sbjct: 545 YFFYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Sed0003445 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 2.6e-136
Identity = 251/565 (44.42%), Postives = 374/565 (66.19%), Query Frame = 0

Query: 16  HTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIYLTTKLHQGTVT 75
           + +DG+VD  GNP L+   G WKAC FI+  E  ER+AYYGI+ NLI YLTTKLHQG V+
Sbjct: 24  YAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVS 83

Query: 76  SANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTLAVSVPSLKPPS 135
           +A NVT W GT ++ P++GA +ADAY GRY T    SGI   GM  LTL+ SVP+LKP  
Sbjct: 84  AATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE 143

Query: 136 CLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEYDPKEKAQKLSF 195
           C+    D C  A+  Q A+FFG LY +ALGTGG KP +S+ GADQFD+ D +E+ +K SF
Sbjct: 144 CI---GDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASF 203

Query: 196 FNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAGTPFYRHKLPSG 255
           FNW+ FSI  G L SS++LV+IQ+N GW LG+G+PT+ + ++I  F  GTP YR + P G
Sbjct: 204 FNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGG 263

Query: 256 SPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSSLRFLNKAAIKT 315
           SP T+++ V+VA+ R  ++ +P D   L+E    +    G+ +I+ T   ++L+KAA+ +
Sbjct: 264 SPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 323

Query: 316 ESKEGAG------KLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQGTTLDR 375
           E +  +G      +LC+VTQVEE K ++RM PI     + S + AQ  T+F++QG  ++ 
Sbjct: 324 EEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNC 383

Query: 376 SVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGIGMILH 435
            +G+  F++PPA+L  F T S++I V +YDR  V + +KFT   +G T +QRMGIG+ + 
Sbjct: 384 KIGS--FQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVS 443

Query: 436 IMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEVAKIEF 495
           ++ M  A+ +E  RL +A   GLV++G    +P+++   +PQ+ ++G A+ F  + ++EF
Sbjct: 444 VLCMAAAAIVEIIRLHMANDLGLVESG--APVPISVLWQIPQYFILGAAEVFYFIGQLEF 503

Query: 496 FYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNE-WILNNLNASHLDYY 555
           FYDQ+P+ M+SL ++ ++ +  +GN+LSS +L+ V+  T RNG E WI +NLN+ HLDY+
Sbjct: 504 FYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYF 563

Query: 556 YALIAVLSAINFIVFLLVSRFYVYK 574
           + L+A LS +N  V+   +  Y  K
Sbjct: 564 FWLLAGLSLVNMAVYFFSAARYKQK 581

BLAST of Sed0003445 vs. ExPASy TrEMBL
Match: A0A5D3D248 (Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001890 PE=3 SV=1)

HSP 1 Score: 973.0 bits (2514), Expect = 5.7e-280
Identity = 486/594 (81.82%), Postives = 547/594 (92.09%), Query Frame = 0

Query: 1   MATTNSVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTN 60
           MA  ++ EE+G +DG+TKDGTVDLKGNPVLRS  GRWKACSFIVVYE+FERMAYYGISTN
Sbjct: 1   MAAASAAEETG-LDGYTKDGTVDLKGNPVLRSQRGRWKACSFIVVYEVFERMAYYGISTN 60

Query: 61  LIIYLTTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMG 120
           LIIYLT KLHQGTVTSANNVTNW+GTVW+MP+LGAY+ADA+LGRYRTFL+AS ICLTGMG
Sbjct: 61  LIIYLTKKLHQGTVTSANNVTNWSGTVWIMPILGAYVADAHLGRYRTFLIASAICLTGMG 120

Query: 121 LLTLAVSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQ 180
           +LTLAVS+PSLKPP CL+VNK+NC++AS LQL VFFGALY LALGTGGTKPNISTMGADQ
Sbjct: 121 ILTLAVSLPSLKPPPCLDVNKENCEKASTLQLVVFFGALYLLALGTGGTKPNISTMGADQ 180

Query: 181 FDEYDPKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILI 240
           FDE+DPKEKAQKLSFFNWWMFSI+FGTLF++TILVYIQDNVGWSLGYGLPTIGLA+SI+I
Sbjct: 181 FDEFDPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISIVI 240

Query: 241 FVAGTPFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRID 300
           F+AGTPFYRHK+P+GSPFT M +VIV AIRNW +P+P+DPK+LHEL+F DY+KQGT RID
Sbjct: 241 FLAGTPFYRHKIPNGSPFTTMVSVIVGAIRNWRIPVPNDPKELHELEFEDYSKQGTFRID 300

Query: 301 STSSLRFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLF 360
           STSS RFLNKAAI    + G+ KLC+VTQVEETKQ+L+MIPIL+CTF+PSTMLAQTHTLF
Sbjct: 301 STSSFRFLNKAAI----RRGSWKLCTVTQVEETKQMLKMIPILICTFIPSTMLAQTHTLF 360

Query: 361 IKQGTTLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQ 420
           IKQGTTLDRSVG+  FKIP ASL +FVTISML+SV IYDR+FVKIMQ+ TKNPRGITLLQ
Sbjct: 361 IKQGTTLDRSVGS-HFKIPAASLASFVTISMLLSVVIYDRVFVKIMQRITKNPRGITLLQ 420

Query: 421 RMGIGMILHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADA 480
           RMGIGMILHI+IMTIASR+E HRL+VAR     +NG  + LPLTIFTLLPQFMLMGTADA
Sbjct: 421 RMGIGMILHILIMTIASRVETHRLKVAR-----ENGSPQVLPLTIFTLLPQFMLMGTADA 480

Query: 481 FIEVAKIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNL 540
           F+EVAKIEFFYDQAPETMKSLGTS+SMTSLGIGNFLS+FLLSTVS IT +NGN W+LNNL
Sbjct: 481 FMEVAKIEFFYDQAPETMKSLGTSFSMTSLGIGNFLSTFLLSTVSHITIKNGNGWVLNNL 540

Query: 541 NASHLDYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQVIADELKDKKFMG 595
           N+SHLDYYYALIAVLS INF VFL+VS+FYVYKAEVS SI+V+ADELKDKKF G
Sbjct: 541 NSSHLDYYYALIAVLSTINFFVFLIVSKFYVYKAEVSTSIKVLADELKDKKFKG 583

BLAST of Sed0003445 vs. ExPASy TrEMBL
Match: A0A1S3AW47 (protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo OX=3656 GN=LOC103483562 PE=3 SV=1)

HSP 1 Score: 973.0 bits (2514), Expect = 5.7e-280
Identity = 486/594 (81.82%), Postives = 547/594 (92.09%), Query Frame = 0

Query: 1   MATTNSVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTN 60
           MA  ++ EE+G +DG+TKDGTVDLKGNPVLRS  GRWKACSFIVVYE+FERMAYYGISTN
Sbjct: 1   MAAASAAEETG-LDGYTKDGTVDLKGNPVLRSQRGRWKACSFIVVYEVFERMAYYGISTN 60

Query: 61  LIIYLTTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMG 120
           LIIYLT KLHQGTVTSANNVTNW+GTVW+MP+LGAY+ADA+LGRYRTFL+AS ICLTGMG
Sbjct: 61  LIIYLTKKLHQGTVTSANNVTNWSGTVWIMPILGAYVADAHLGRYRTFLIASAICLTGMG 120

Query: 121 LLTLAVSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQ 180
           +LTLAVS+PSLKPP CL+VNK+NC++AS LQL VFFGALY LALGTGGTKPNISTMGADQ
Sbjct: 121 ILTLAVSLPSLKPPPCLDVNKENCEKASTLQLVVFFGALYLLALGTGGTKPNISTMGADQ 180

Query: 181 FDEYDPKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILI 240
           FDE+DPKEKAQKLSFFNWWMFSI+FGTLF++TILVYIQDNVGWSLGYGLPTIGLA+SI+I
Sbjct: 181 FDEFDPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISIVI 240

Query: 241 FVAGTPFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRID 300
           F+AGTPFYRHK+P+GSPFT M +VIV AIRNW +P+P+DPK+LHEL+F DY+KQGT RID
Sbjct: 241 FLAGTPFYRHKIPNGSPFTTMVSVIVGAIRNWRIPVPNDPKELHELEFEDYSKQGTFRID 300

Query: 301 STSSLRFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLF 360
           STSS RFLNKAAI    + G+ KLC+VTQVEETKQ+L+MIPIL+CTF+PSTMLAQTHTLF
Sbjct: 301 STSSFRFLNKAAI----RRGSWKLCTVTQVEETKQMLKMIPILICTFIPSTMLAQTHTLF 360

Query: 361 IKQGTTLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQ 420
           IKQGTTLDRSVG+  FKIP ASL +FVTISML+SV IYDR+FVKIMQ+ TKNPRGITLLQ
Sbjct: 361 IKQGTTLDRSVGS-HFKIPAASLASFVTISMLLSVVIYDRVFVKIMQRITKNPRGITLLQ 420

Query: 421 RMGIGMILHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADA 480
           RMGIGMILHI+IMTIASR+E HRL+VAR     +NG  + LPLTIFTLLPQFMLMGTADA
Sbjct: 421 RMGIGMILHILIMTIASRVETHRLKVAR-----ENGSPQVLPLTIFTLLPQFMLMGTADA 480

Query: 481 FIEVAKIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNL 540
           F+EVAKIEFFYDQAPETMKSLGTS+SMTSLGIGNFLS+FLLSTVS IT +NGN W+LNNL
Sbjct: 481 FMEVAKIEFFYDQAPETMKSLGTSFSMTSLGIGNFLSTFLLSTVSHITIKNGNGWVLNNL 540

Query: 541 NASHLDYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQVIADELKDKKFMG 595
           N+SHLDYYYALIAVLS INF VFL+VS+FYVYKAEVS SI+V+ADELKDKKF G
Sbjct: 541 NSSHLDYYYALIAVLSTINFFVFLIVSKFYVYKAEVSTSIKVLADELKDKKFKG 583

BLAST of Sed0003445 vs. ExPASy TrEMBL
Match: A0A6J1IQK5 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479112 PE=3 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.7e-279
Identity = 486/591 (82.23%), Postives = 542/591 (91.71%), Query Frame = 0

Query: 5   NSVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIY 64
           ++ EESG VD +TKDGTVDLKGNPVLRS  GRWKACSFIVVYE+FERMAYYGISTNLII+
Sbjct: 4   SAAEESG-VDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNLIIF 63

Query: 65  LTTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTL 124
           LT KLHQGTV SANNVTNW+GTVW+MP+LGAYIADA+LGRYRTFL+AS ICLTGMGLLTL
Sbjct: 64  LTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGLLTL 123

Query: 125 AVSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEY 184
           AVS+PSLKPP CL+VNK NC+ AS LQLAVFFGALY LALGTGGTKPNIST+GADQFDE+
Sbjct: 124 AVSLPSLKPPPCLDVNKGNCKPASTLQLAVFFGALYMLALGTGGTKPNISTIGADQFDEF 183

Query: 185 DPKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAG 244
            PKEKAQKLSFFNWWMFSI+FGTLF++TILVYIQDNVGWSLGYGLPTIGLA+SILIFVAG
Sbjct: 184 HPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAG 243

Query: 245 TPFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSS 304
           TPFYRHKLP+GSPFTKMA+VIVAA+RNW LPLP+DPK+LHEL F +Y KQG  RIDST S
Sbjct: 244 TPFYRHKLPTGSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFRIDSTPS 303

Query: 305 LRFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQG 364
           LRFLNKAAI+  S + + KLC+VTQVEETKQ+LRMIP+L+CTF+PSTMLAQTHTLFIKQG
Sbjct: 304 LRFLNKAAIRRGSSD-SWKLCTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTLFIKQG 363

Query: 365 TTLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGI 424
           TTLDRS+G+  F+IPPASL AFVTISML+SV IYDR+FVKIMQ+ TKNPRGITLLQRMGI
Sbjct: 364 TTLDRSIGS-HFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQRMGI 423

Query: 425 GMILHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEV 484
           GMILH++IM IASR+ERHRL+VAR NG V NG  + LPLTIFTLLPQFML+G ADAF EV
Sbjct: 424 GMILHVLIMIIASRVERHRLDVARQNGQVGNGSGQALPLTIFTLLPQFMLVGVADAFTEV 483

Query: 485 AKIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNLNASH 544
           AKIEFFYDQAPE+MKSLGTSYSMTS+GIGNFLSSFLLSTVS IT ++GN WI+NNLNASH
Sbjct: 484 AKIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKHGNGWIMNNLNASH 543

Query: 545 LDYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQVIADELKDKKFMGF 596
           LDYYYA +AVLSAINF +FLL+S+FYVYKAEVSGSI+ +ADELKDKK   F
Sbjct: 544 LDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKALADELKDKKLKPF 591

BLAST of Sed0003445 vs. ExPASy TrEMBL
Match: A0A6J1E956 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431935 PE=3 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 3.7e-279
Identity = 486/591 (82.23%), Postives = 541/591 (91.54%), Query Frame = 0

Query: 5   NSVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIY 64
           ++ EESG VD +TKDGTVDLKGNPVLRS  GRWKACSFIVVYE+FERMAYYGISTNLII+
Sbjct: 4   SAAEESG-VDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNLIIF 63

Query: 65  LTTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTL 124
           LT KLHQGTV SANNVTNW+GTVW+MP+LGAYIADA+LGRYRTFL+AS ICLTGMGLLTL
Sbjct: 64  LTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGLLTL 123

Query: 125 AVSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEY 184
           AVS+PSLKPP CL+VNK NC+ AS LQLAVFFGALY LALGTGGTKPNIST+GADQFDE+
Sbjct: 124 AVSLPSLKPPPCLDVNKGNCKPASTLQLAVFFGALYMLALGTGGTKPNISTIGADQFDEF 183

Query: 185 DPKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAG 244
            PKEKAQKLSFFNWWMFSI+FGTLF++TILVYIQDNVGWSLGYGLPTIGLA+SILIFVAG
Sbjct: 184 HPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAG 243

Query: 245 TPFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSS 304
           TPFYRHKLP+GSPFTKMA+VIVAA+RNW LPLP+DPK+LHEL F +Y KQG  RIDST S
Sbjct: 244 TPFYRHKLPTGSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFRIDSTPS 303

Query: 305 LRFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQG 364
           LRFLNKAAI+  S + + KLC+VTQVEETKQ+LRMIP+L+CTF+PSTMLAQTHTLFIKQG
Sbjct: 304 LRFLNKAAIRRGSSD-SWKLCTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTLFIKQG 363

Query: 365 TTLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGI 424
           TTLDRS+G+  F+IPPASL AFVTISML+SV IYDR+FVKIMQ+ TKNPRGITLLQRMGI
Sbjct: 364 TTLDRSIGS-HFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQRMGI 423

Query: 425 GMILHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEV 484
           GMILH++IM IASR+ERHRL+VAR NG V N   + LPLTIFTLLPQFML+G ADAF EV
Sbjct: 424 GMILHVLIMIIASRVERHRLDVARQNGQVGNASGQALPLTIFTLLPQFMLVGVADAFTEV 483

Query: 485 AKIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNLNASH 544
           AKIEFFYDQAPE+MKSLGTSYSMTS+GIGNFLSSFLLSTVS IT + GN WI+NNLNASH
Sbjct: 484 AKIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKRGNGWIMNNLNASH 543

Query: 545 LDYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQVIADELKDKKFMGF 596
           LDYYYA +AVLSAINF +FLL+S+FYVYKAEVSGSI+V+ADELKDKK   F
Sbjct: 544 LDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKVLADELKDKKLKPF 591

BLAST of Sed0003445 vs. ExPASy TrEMBL
Match: A0A0A0L706 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G134770 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.8e-276
Identity = 480/590 (81.36%), Postives = 542/590 (91.86%), Query Frame = 0

Query: 5   NSVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIY 64
           ++ EE+G +D +TKDGTVDLKGNPVLRS  GRWKACSFIVVYE+FERMAYYGISTNLIIY
Sbjct: 4   SAAEETG-LDDYTKDGTVDLKGNPVLRSQRGRWKACSFIVVYEVFERMAYYGISTNLIIY 63

Query: 65  LTTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTL 124
           LT KLHQGTVTSANNVTNW+GTVW+MP+LGAY+ADA+LGRYRTFLVAS ICLTGMG+LTL
Sbjct: 64  LTKKLHQGTVTSANNVTNWSGTVWIMPILGAYVADAHLGRYRTFLVASAICLTGMGILTL 123

Query: 125 AVSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEY 184
           AVS+PSLKPP C +VNK+NC++AS LQLAVFFGALY LALGTGGTKPNISTMGADQFD++
Sbjct: 124 AVSLPSLKPPPCSDVNKENCKEASTLQLAVFFGALYMLALGTGGTKPNISTMGADQFDDF 183

Query: 185 DPKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAG 244
            PKEKAQKLSFFNWWMFSI+FGTLF++TILVYIQDNVGWSLGYGLPTIGLA+SI+IF+AG
Sbjct: 184 HPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISIVIFLAG 243

Query: 245 TPFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSS 304
           TPFYRHK+P+GSPFT M +VIV AIRNW +P+P+DPK+LHEL+F +Y+KQGT RIDSTSS
Sbjct: 244 TPFYRHKIPNGSPFTTMVSVIVGAIRNWRIPVPNDPKELHELEFEEYSKQGTFRIDSTSS 303

Query: 305 LRFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQG 364
            RFLNKAA+    + G+ KLC+VTQVEETKQIL+MIPIL+CTF+PSTMLAQTHTLFIKQG
Sbjct: 304 FRFLNKAAV----RRGSWKLCTVTQVEETKQILKMIPILICTFIPSTMLAQTHTLFIKQG 363

Query: 365 TTLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGI 424
           TTLDRSVG+  FKIP ASL +FVTISML+SV IYDR+FVK+MQ+ TKNPRGITLLQRMGI
Sbjct: 364 TTLDRSVGS-HFKIPAASLASFVTISMLVSVVIYDRVFVKVMQRITKNPRGITLLQRMGI 423

Query: 425 GMILHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEV 484
           GMILHI+IMTIASRIE HRL+VAR     +NG  + LPLTIFTLLPQFMLMGTADAF+EV
Sbjct: 424 GMILHILIMTIASRIETHRLKVAR-----ENGSPQVLPLTIFTLLPQFMLMGTADAFMEV 483

Query: 485 AKIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNLNASH 544
           AKIEFFYDQAPETMKSLGTS+SMTSLGIGNFLS+FLLSTVS IT +NGN WILNNLN+SH
Sbjct: 484 AKIEFFYDQAPETMKSLGTSFSMTSLGIGNFLSTFLLSTVSHITIKNGNGWILNNLNSSH 543

Query: 545 LDYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQVIADELKDKKFMG 595
           LDYYYALIAVLS INF VFL++S+ YVYKAEVS SI+V+ADELKDKKF G
Sbjct: 544 LDYYYALIAVLSTINFFVFLIISKLYVYKAEVSTSIKVLADELKDKKFKG 582

BLAST of Sed0003445 vs. TAIR 10
Match: AT5G46050.1 (peptide transporter 3 )

HSP 1 Score: 790.0 bits (2039), Expect = 1.3e-228
Identity = 395/585 (67.52%), Postives = 481/585 (82.22%), Query Frame = 0

Query: 6   SVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIYL 65
           +VEE G  D +TKDGTVDL+GNPV RS  GRWKACSF+VVYE+FERMAYYGIS+NL IY+
Sbjct: 2   TVEEVG--DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYM 61

Query: 66  TTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTLA 125
           TTKLHQGTV S+NNVTNW GT W+ P+LGAY+ DA LGRY TF+++  I  +GM +LTL+
Sbjct: 62  TTKLHQGTVKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLS 121

Query: 126 VSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEYD 185
           V++P +KPP C   N +NC++AS LQLAVFFGALYTLA+GTGGTKPNIST+GADQFD +D
Sbjct: 122 VTIPGIKPPECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFD 181

Query: 186 PKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAGT 245
           PKEK QKLSFFNWWMFSI+FGTLF++T+LVY+QDNVGW+LGYGLPT+GLA+SI IF+ GT
Sbjct: 182 PKEKTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGT 241

Query: 246 PFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSSL 305
           PFYRHKLP+GSPFTKMA VIVA+ R  N P+  D    HEL   +Y ++G   I  T SL
Sbjct: 242 PFYRHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTPSL 301

Query: 306 RFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQGT 365
           RFL++A++KT +      LC+ T+VEETKQ+LRM+P+L  TF+PS MLAQ +TLF+KQGT
Sbjct: 302 RFLDRASLKTGTNH-KWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQGT 361

Query: 366 TLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGIG 425
           TLDR V  G F IPPASL+ FVT+SMLIS+ +YDR+FVKI +KFT NPRGITLLQRMGIG
Sbjct: 362 TLDRKV-TGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIG 421

Query: 426 MILHIMIMTIASRIERHRLEVARTNGLV-QNGEHEQLPLTIFTLLPQFMLMGTADAFIEV 485
           +I HI+IM +AS  ER+RL+VA  +GL+ Q G   +LPLTIF LLPQF+LMG AD+F+EV
Sbjct: 422 LIFHILIMIVASVTERYRLKVAADHGLIHQTG--VKLPLTIFALLPQFVLMGMADSFLEV 481

Query: 486 AKIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNLNASH 545
           AK+EFFYDQAPE+MKSLGTSYS TSL IGNF+SSFLLSTVS ITK+ G  WILNNLN S 
Sbjct: 482 AKLEFFYDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNLNESR 541

Query: 546 LDYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQVIADELKD 590
           LDYYY   AVL+ +NF++FL+V +FYVY+AEV+ S+ V   E+K+
Sbjct: 542 LDYYYLFFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMKE 580

BLAST of Sed0003445 vs. TAIR 10
Match: AT5G46040.1 (Major facilitator superfamily protein )

HSP 1 Score: 752.7 bits (1942), Expect = 2.3e-217
Identity = 380/577 (65.86%), Postives = 464/577 (80.42%), Query Frame = 0

Query: 6   SVEESGVVDGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIYL 65
           +VEE G  D +TKDGTVDL+GN V RS  GRWKACSF+VVYE+FERMAYYGIS+NL+IY+
Sbjct: 2   TVEEVG--DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYM 61

Query: 66  TTKLHQGTVTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTLA 125
           TTKLHQGTV S+NNVTNW GT W+ P+LGAY+ADA+ GRY TF+++S I L GM LLTL+
Sbjct: 62  TTKLHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLS 121

Query: 126 VSVPSLKPPSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEYD 185
           VS+P LKPP C   N +NC++AS +QLAVFFGALYTLA+GTGGTKPNIST+GADQFDE+D
Sbjct: 122 VSLPGLKPPKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFD 181

Query: 186 PKEKAQKLSFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAGT 245
           PK+K  K SFFNWWMFSI+FGT F++T+LVY+QDNVGW++GYGL T+GLA SI IF+ GT
Sbjct: 182 PKDKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGT 241

Query: 246 PFYRHKLPSGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSSL 305
             YRHKLP GSPFTKMA VIVA++R    P+ SD  + +EL   +Y  +    I STSSL
Sbjct: 242 RLYRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTSSL 301

Query: 306 RFLNKAAIKTESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQGT 365
           RFLN+A++KT S     +LC++T+VEETKQ+L+M+P+L  TF+PS MLAQ  TLFIKQGT
Sbjct: 302 RFLNRASLKTGSTH-KWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQGT 361

Query: 366 TLDRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGIG 425
           TLDR + N  F IPPASL  F T SML+S+ IYDR+FVK M+K T NPRGITLLQRMGIG
Sbjct: 362 TLDRRLTN-NFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIG 421

Query: 426 MILHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEVA 485
           MILHI+IM IAS  ER+RL+VA  +GL        +PL+IFTLLPQ++LMG ADAFIE+A
Sbjct: 422 MILHILIMIIASITERYRLKVAAEHGLTHQ-TAVPIPLSIFTLLPQYVLMGLADAFIEIA 481

Query: 486 KIEFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNEWILNNLNASHL 545
           K+EFFYDQAPE+MKSLGTSY+ TS+ +G F+SS LLS+VS+ITK+ G  WI NNLN S L
Sbjct: 482 KLEFFYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNLNESRL 541

Query: 546 DYYYALIAVLSAINFIVFLLVSRFYVYKAEVSGSIQV 583
           D YY   AVL+ +NFI+FL+V RFY Y+A+V+ S  V
Sbjct: 542 DNYYMFFAVLNLLNFILFLVVIRFYEYRADVTQSANV 573

BLAST of Sed0003445 vs. TAIR 10
Match: AT2G40460.1 (Major facilitator superfamily protein )

HSP 1 Score: 565.5 bits (1456), Expect = 5.3e-161
Identity = 290/562 (51.60%), Postives = 400/562 (71.17%), Query Frame = 0

Query: 16  HTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIYLTTKLHQGTVT 75
           +T+DGTVDL+G PVL S  GRW+ACSF++ YE FERMA+YGI++NL+ YLT +LH+ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 76  SANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTLAVSVPSLKPPS 135
           S  NV NW+G VW+ P+ GAYIAD+Y+GR+ TF  +S I + GM LLT+AV+V SL+ P+
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLR-PT 126

Query: 136 CLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEYDPKEKAQKLSF 195
           C       C +AS LQ+  F+ +LYT+A+G GGTKPNIST GADQFD Y  +EK QK+SF
Sbjct: 127 C---ENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVSF 186

Query: 196 FNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAGTPFYRHKLPSG 255
           FNWWMFS + G LF++  LVYIQ+N+GW LGYG+PT+GL VS+++F  GTPFYRHK+   
Sbjct: 187 FNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIKT 246

Query: 256 SPFTK-MANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSSLRFLNKAAIK 315
               K +  V +AA +N  L  P D  +L+ELD H Y   G  ++  T   RFL+KAAIK
Sbjct: 247 DNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAIK 306

Query: 316 TESKEGAGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQGTTLDRSVGNG 375
           T S+      C+VT+VE  K++L +I I + T +PST+ AQ +TLF+KQGTTLDR +G+ 
Sbjct: 307 TSSRVP----CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRKIGS- 366

Query: 376 GFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGIGMILHIMIMT 435
            F+IP ASL +FVT+SML+SV +YD+ FV  M+K T NPRGITLLQR+G+G  + I+ + 
Sbjct: 367 NFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIVAIA 426

Query: 436 IASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEVAKIEFFYDQA 495
           IAS +E  R+ V +   +      + +P++IF LLPQ+ L+G  D F  +  +EFFYDQ+
Sbjct: 427 IASAVEVKRMRVIKEFHI--TSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQS 486

Query: 496 PETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRIT-KRNGNEWILNNLNASHLDYYYALIA 555
           PE M+SLGT++  + +G+GNFL+SFL++ + +IT K  G  WI NNLN S LDYYY  + 
Sbjct: 487 PEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGFLV 546

Query: 556 VLSAINFIVFLLVSRFYVYKAE 576
           V+S +N  +F+  +  YVYK++
Sbjct: 547 VISIVNMGLFVWAASKYVYKSD 557

BLAST of Sed0003445 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 501.1 bits (1289), Expect = 1.2e-141
Identity = 262/570 (45.96%), Postives = 387/570 (67.89%), Query Frame = 0

Query: 14  DGHTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIYLTTKLHQGT 73
           D +T+DGTVD+  NP  +   G WKAC FI+  E  ER+AYYG+ TNL+ YL ++L+QG 
Sbjct: 5   DVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 64

Query: 74  VTSANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTLAVSVPSLKP 133
            T+ANNVTNW+GT ++ P++GA+IADAYLGRY T      I ++GM LLTL+ SVP LKP
Sbjct: 65  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 124

Query: 134 PSCLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEYDPKEKAQKL 193
            +C   N D C   S  Q AVFF ALY +ALGTGG KP +S+ GADQFDE D  EK +K 
Sbjct: 125 GNC---NADTCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 184

Query: 194 SFFNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAGTPFYRHKLP 253
           SFFNW+ FSI  G L ++T+LV+IQ NVGW  G+G+PT+ + +++  F  G+ FYR + P
Sbjct: 185 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 244

Query: 254 SGSPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSSLRFLNKAAI 313
            GSP T++  VIVAA R  ++ +P D   L E    +   +G+ ++  T +L+F +KAA+
Sbjct: 245 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV 304

Query: 314 KTES---KEG---AGKLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQGTTL 373
           +++S   K+G     +LCSVTQVEE K I+ ++P+     + +T+ +Q  T+F+ QG T+
Sbjct: 305 ESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTM 364

Query: 374 DRSVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGIGMI 433
           D+ +G   F+IP ASL+ F T+S+L    +YD+  + + +KFT+N RG T LQRMGIG++
Sbjct: 365 DQHMGK-NFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 424

Query: 434 LHIMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEVAKI 493
           + I  M  A  +E  RL+  +T+      + +Q+ ++IF  +PQ++L+G A+ F  + ++
Sbjct: 425 VSIFAMITAGVLEVVRLDYVKTHNAY---DQKQIHMSIFWQIPQYLLIGCAEVFTFIGQL 484

Query: 494 EFFYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNE-WILNNLNASHLD 553
           EFFYDQAP+ M+SL ++ S+T++ +GN+LS+ L++ V +ITK+NG   WI +NLN  HLD
Sbjct: 485 EFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLD 544

Query: 554 YYYALIAVLSAINFIVFLLVSRFYVYKAEV 577
           Y++ L+A LS +NF+V+L +S+ Y YK  V
Sbjct: 545 YFFYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Sed0003445 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 487.3 bits (1253), Expect = 1.8e-137
Identity = 251/565 (44.42%), Postives = 374/565 (66.19%), Query Frame = 0

Query: 16  HTKDGTVDLKGNPVLRSNGGRWKACSFIVVYEMFERMAYYGISTNLIIYLTTKLHQGTVT 75
           + +DG+VD  GNP L+   G WKAC FI+  E  ER+AYYGI+ NLI YLTTKLHQG V+
Sbjct: 24  YAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVS 83

Query: 76  SANNVTNWTGTVWMMPVLGAYIADAYLGRYRTFLVASGICLTGMGLLTLAVSVPSLKPPS 135
           +A NVT W GT ++ P++GA +ADAY GRY T    SGI   GM  LTL+ SVP+LKP  
Sbjct: 84  AATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE 143

Query: 136 CLEVNKDNCQQASKLQLAVFFGALYTLALGTGGTKPNISTMGADQFDEYDPKEKAQKLSF 195
           C+    D C  A+  Q A+FFG LY +ALGTGG KP +S+ GADQFD+ D +E+ +K SF
Sbjct: 144 CI---GDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASF 203

Query: 196 FNWWMFSIYFGTLFSSTILVYIQDNVGWSLGYGLPTIGLAVSILIFVAGTPFYRHKLPSG 255
           FNW+ FSI  G L SS++LV+IQ+N GW LG+G+PT+ + ++I  F  GTP YR + P G
Sbjct: 204 FNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGG 263

Query: 256 SPFTKMANVIVAAIRNWNLPLPSDPKQLHELDFHDYTKQGTVRIDSTSSLRFLNKAAIKT 315
           SP T+++ V+VA+ R  ++ +P D   L+E    +    G+ +I+ T   ++L+KAA+ +
Sbjct: 264 SPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 323

Query: 316 ESKEGAG------KLCSVTQVEETKQILRMIPILVCTFLPSTMLAQTHTLFIKQGTTLDR 375
           E +  +G      +LC+VTQVEE K ++RM PI     + S + AQ  T+F++QG  ++ 
Sbjct: 324 EEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNC 383

Query: 376 SVGNGGFKIPPASLTAFVTISMLISVAIYDRIFVKIMQKFTKNPRGITLLQRMGIGMILH 435
            +G+  F++PPA+L  F T S++I V +YDR  V + +KFT   +G T +QRMGIG+ + 
Sbjct: 384 KIGS--FQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVS 443

Query: 436 IMIMTIASRIERHRLEVARTNGLVQNGEHEQLPLTIFTLLPQFMLMGTADAFIEVAKIEF 495
           ++ M  A+ +E  RL +A   GLV++G    +P+++   +PQ+ ++G A+ F  + ++EF
Sbjct: 444 VLCMAAAAIVEIIRLHMANDLGLVESG--APVPISVLWQIPQYFILGAAEVFYFIGQLEF 503

Query: 496 FYDQAPETMKSLGTSYSMTSLGIGNFLSSFLLSTVSRITKRNGNE-WILNNLNASHLDYY 555
           FYDQ+P+ M+SL ++ ++ +  +GN+LSS +L+ V+  T RNG E WI +NLN+ HLDY+
Sbjct: 504 FYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYF 563

Query: 556 YALIAVLSAINFIVFLLVSRFYVYK 574
           + L+A LS +N  V+   +  Y  K
Sbjct: 564 FWLLAGLSLVNMAVYFFSAARYKQK 581