Sed0003404 (gene) Chayote v1

Overview
NameSed0003404
Typegene
OrganismSechium edule (Chayote v1)
DescriptionPectinesterase
LocationLG03: 1452167 .. 1455007 (+)
RNA-Seq ExpressionSed0003404
SyntenySed0003404
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTGAACCAAAAAATCTAAATGGACCGAAAGAAACTAGAAAGAGTTAAATGATTGAAGGATCGAATTCCAAACTTTAGGACATATAAAATTCCGTCGAACTTTTTTCCTTTTTTGTGTATTCGTATTTAATCGAAAATGTGATCTACAGATGCCATCTCAATGTTATGGATTGAAATGCCAAATTTGGTTCCATAATTAGATCTTCATCTTCAACAAAGAGGCGCGAGCTTCGAGACAGGGTTCCATGGCGGCGCCGCCCAGAGTTATCACGGTGGCGCACGACGGCGGTGGCGATTTCACGACGGTTCAAGAAGCCATTGACGCGGTTCCATTCAGCAATACCTGCCGGACTGTGATCCGTATTGCTCCTGGCATCTACAGGCAGCCGCTCTACGTGCCCAAGACCAAGAACTTCATCACCTTCGCCGGTTTGTGCCCTGAAACTACGATTCTCACTTGGGACAACACTGCCACCAAAATTAATCACCACCAAGTTCGTTTCCCATGATTCTTTTCTCATTTTATGATCTGGGTTTGTGTTAATTTTGCGAGATTGTAATGTTTTCATCATGTGTTAGGCTTCTCGTGTTATTGGAACTGGTACTTTTGGCTGTGGGAGTACGATTGTTGAAGGGGAGGATTTCATTGCTGAGAATATCACGTTTGAGAATTCTTCCCCTCAGGTCGTTTGATTATATTTATCTTTACTTTGGTTTCATATCACACATATGTCCTCGAGTGGCGACCTCGTCGTTTTTAGAGGCTTCTAGTACATGTTGGAAATCCACAAATGAGCTTAATTGTAAAATCCTTCCAGGTTATGCCCTCAAGTATAGTAGGGCGTAGTTCTGATTTCCGGTTATCGAAAAAGAAGGAAATTATATCAGTGATATGGAGATGGAATGGTTATGGATAGCCCCTTTTTAGTTCATAGTTTGAGGTTGATTTTGAATGTTAATCTCTGTGCTATCAAATTCGTTTTGTAGTTGCTATTGTTTTATTTTGTGTAGTGGCTTCTTTGTTTTCTCCAGGGTTCTGGACAAGCTGTGGCTATTAGAGTTACAGCAGATCGTTGTGCCTTTTACAATTGCAGGTTTCTCGGCTGGCAGGTATATTACAATCATCTAATTCAATGAGTTTATGCCTAGTTAGTTCAAGAGTTTAAACAATATTCTGGAACGACCTACTGGTTTTTGTTATACTTCATTAATTTTCCTTTACGTGGTACACTAATGTTCTGTTAGCTACATTCAAAGGCAAAAGCGATAAGTCATTTTATTTAAGAGATCGTGAAGAAATAAGAGTTTATATAGTGCAGCTATATTTAGTGTTCAACTTCTTTTAGCTTTGTTGGGTCAGTTGCCAATCGAGCTTTCTTTGCTCTTACTCACTGGAACTCTGTTGGGATTTAAAGTTCGATGCCGTTGCCAATATCCAACAGCTCTATCCTTTGGCTAGTTCAATCAGCTAAGACCATTCACGATCACAGTAGATAGATTTTTTGTACTTGTATTGCCTGCTATTGTTCTTCTGCTGAAATACTTCTGGATGTCTGGGAAATAGCTATTCATATTTTCATTTTTCCCTTTTGATTTGATGAAGGACACTCTGTACCTACATCATGGAAAACAGTATTTGAAGGATTGCTATATTGAAGGAAGTGTGGACTTCATATTTGGCAACAGCACTGCTTTATTAGAGAACTGTCATGTCCACTGCAAGTCGCGTGGCTTTATTACCGCACAAAGCAGGAAATCGTCTCAAGAGACGACAGGCTATGTGTTCTTGAGGTGACGTTGGCGGAAATTATGTTACTCTTGTTCAAAATTTCTGGGTGTATCGCTGATTACTGAAACACCGATTTTTCTGTGACTCCGCGACATCGATCTTCTCTTTTTAGGCAATAACTTAATAGTTGAATTCTTGCATCCTTTTGGTGGTGGTTAGTTTTAGCAATTATATCATTCACATTTTAGAAGCTAAGATAAAGTGTGGTTATTTTTGCCCTTCTTCAACATTTTGTATCATTATGAGGTTTAGGAAAATAAAGTCACCTTAGGATTATGATTCAAACTCTATGTTATGTATCCTTTCTTTGTGTTTCACCTTGAAAATCTCTACTGATTTGTTTCTCAATTAATATAGATGTGTGATCACGGGCAGTGGCGAGGCTTCGCACACTCATCTCGGGCGACCTTGGGGACCATTTGCAAGGGTGGTCTTTGCTTACACATACATGGATGCATGTATTAAACCAGCAGGCTGGGACAACTGGGGAAAAATTGAAAATGAGAGAACTGCTTGCTTTTACGAGTACAAGTATGCCATGTTTAATGCCTTTTGCCTTATTGTTACACAGTATCTATGCTTATTACGACTTCGAACAACGATTATACTATGAATGGGATCATCGCTGAATGCCTAGATCACATGATTGGAGACTCGTGTTTTTCGGTACAGGTGTTTCGGGCCAGGGAGTTTCTCAACGAAAAGGGTGTGTTGGGCAAGGGAATTAATGGATGAAGAAGCTGATGAGTTCCTTCTGCATCAATTTATCGATCCCGATATCGAAAGGCGTTGGCTATGCCAGAGAATGGCATTGAGGATTCCATTTTCTGCTTAACGATGGCATATTAAAGCTTACAGAACATAAAATCAGCAAAATATGCTCAGGGTTTCTTTATGGCCTGAGATTGTGATTTAGTAATGTGTTGACGAATTTTTCATACTACTTTGAGCTTTATCTATTGTGGTCTTTGAATGTCTTCCGAAACTTTCAATAAAACTTGAATTTAGTCCAAGTTCAAGTTAATTTTTATTGAAATTCATTCCATAATAA

mRNA sequence

AATTGAACCAAAAAATCTAAATGGACCGAAAGAAACTAGAAAGAGTTAAATGATTGAAGGATCGAATTCCAAACTTTAGGACATATAAAATTCCGTCGAACTTTTTTCCTTTTTTGTGTATTCGTATTTAATCGAAAATGTGATCTACAGATGCCATCTCAATGTTATGGATTGAAATGCCAAATTTGGTTCCATAATTAGATCTTCATCTTCAACAAAGAGGCGCGAGCTTCGAGACAGGGTTCCATGGCGGCGCCGCCCAGAGTTATCACGGTGGCGCACGACGGCGGTGGCGATTTCACGACGGTTCAAGAAGCCATTGACGCGGTTCCATTCAGCAATACCTGCCGGACTGTGATCCGTATTGCTCCTGGCATCTACAGGCAGCCGCTCTACGTGCCCAAGACCAAGAACTTCATCACCTTCGCCGGTTTGTGCCCTGAAACTACGATTCTCACTTGGGACAACACTGCCACCAAAATTAATCACCACCAAGCTTCTCGTGTTATTGGAACTGGTACTTTTGGCTGTGGGAGTACGATTGTTGAAGGGGAGGATTTCATTGCTGAGAATATCACGTTTGAGAATTCTTCCCCTCAGGGTTCTGGACAAGCTGTGGCTATTAGAGTTACAGCAGATCGTTGTGCCTTTTACAATTGCAGGTTTCTCGGCTGGCAGGACACTCTGTACCTACATCATGGAAAACAGTATTTGAAGGATTGCTATATTGAAGGAAGTGTGGACTTCATATTTGGCAACAGCACTGCTTTATTAGAGAACTGTCATGTCCACTGCAAGTCGCGTGGCTTTATTACCGCACAAAGCAGGAAATCGTCTCAAGAGACGACAGGCTATGTGTTCTTGAGATGTGTGATCACGGGCAGTGGCGAGGCTTCGCACACTCATCTCGGGCGACCTTGGGGACCATTTGCAAGGGTGGTCTTTGCTTACACATACATGGATGCATGTATTAAACCAGCAGGCTGGGACAACTGGGGAAAAATTGAAAATGAGAGAACTGCTTGCTTTTACGAGTACAAGTGTTTCGGGCCAGGGAGTTTCTCAACGAAAAGGGTGTGTTGGGCAAGGGAATTAATGGATGAAGAAGCTGATGAGTTCCTTCTGCATCAATTTATCGATCCCGATATCGAAAGGCGTTGGCTATGCCAGAGAATGGCATTGAGGATTCCATTTTCTGCTTAACGATGGCATATTAAAGCTTACAGAACATAAAATCAGCAAAATATGCTCAGGGTTTCTTTATGGCCTGAGATTGTGATTTAGTAATGTGTTGACGAATTTTTCATACTACTTTGAGCTTTATCTATTGTGGTCTTTGAATGTCTTCCGAAACTTTCAATAAAACTTGAATTTAGTCCAAGTTCAAGTTAATTTTTATTGAAATTCATTCCATAATAA

Coding sequence (CDS)

ATGGCGGCGCCGCCCAGAGTTATCACGGTGGCGCACGACGGCGGTGGCGATTTCACGACGGTTCAAGAAGCCATTGACGCGGTTCCATTCAGCAATACCTGCCGGACTGTGATCCGTATTGCTCCTGGCATCTACAGGCAGCCGCTCTACGTGCCCAAGACCAAGAACTTCATCACCTTCGCCGGTTTGTGCCCTGAAACTACGATTCTCACTTGGGACAACACTGCCACCAAAATTAATCACCACCAAGCTTCTCGTGTTATTGGAACTGGTACTTTTGGCTGTGGGAGTACGATTGTTGAAGGGGAGGATTTCATTGCTGAGAATATCACGTTTGAGAATTCTTCCCCTCAGGGTTCTGGACAAGCTGTGGCTATTAGAGTTACAGCAGATCGTTGTGCCTTTTACAATTGCAGGTTTCTCGGCTGGCAGGACACTCTGTACCTACATCATGGAAAACAGTATTTGAAGGATTGCTATATTGAAGGAAGTGTGGACTTCATATTTGGCAACAGCACTGCTTTATTAGAGAACTGTCATGTCCACTGCAAGTCGCGTGGCTTTATTACCGCACAAAGCAGGAAATCGTCTCAAGAGACGACAGGCTATGTGTTCTTGAGATGTGTGATCACGGGCAGTGGCGAGGCTTCGCACACTCATCTCGGGCGACCTTGGGGACCATTTGCAAGGGTGGTCTTTGCTTACACATACATGGATGCATGTATTAAACCAGCAGGCTGGGACAACTGGGGAAAAATTGAAAATGAGAGAACTGCTTGCTTTTACGAGTACAAGTGTTTCGGGCCAGGGAGTTTCTCAACGAAAAGGGTGTGTTGGGCAAGGGAATTAATGGATGAAGAAGCTGATGAGTTCCTTCTGCATCAATTTATCGATCCCGATATCGAAAGGCGTTGGCTATGCCAGAGAATGGCATTGAGGATTCCATTTTCTGCTTAA

Protein sequence

MAAPPRVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITFAGLCPETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCHVHCKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDACIKPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFIDPDIERRWLCQRMALRIPFSA
Homology
BLAST of Sed0003404 vs. NCBI nr
Match: XP_022134347.1 (pectinesterase 31 [Momordica charantia])

HSP 1 Score: 637.1 bits (1642), Expect = 8.1e-179
Identity = 293/318 (92.14%), Postives = 305/318 (95.91%), Query Frame = 0

Query: 1   MAAPPRVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITF 60
           MAAPPRVITVAHDG GDF TVQEAIDAVPFSNTCRTVIR++PGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVITVAHDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLCPETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL PE+T+LTWDNTATKINHHQASR+IGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLSPESTVLTWDNTATKINHHQASRIIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCH 180
           GQAVAIRVTADRCAFYNC+FLGWQDTLYLH+GKQYLKDCYIEGSVDFIFGNSTALLE+CH
Sbjct: 121 GQAVAIRVTADRCAFYNCKFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 VHCKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDA 240
           VHCKS GFITAQSRKSSQETTGYVFLRCVITGSG  S+ HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 VHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGNSYIHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFIDPD 300
           CIKPAGWDNWGKIENERTACFYEYKCFGPGS   KRVCWAREL+DEE DEFLLHQFIDP+
Sbjct: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSCQMKRVCWARELVDEETDEFLLHQFIDPN 300

Query: 301 IERRWLCQRMALRIPFSA 319
           +ER WLCQRMA RIPFSA
Sbjct: 301 VERPWLCQRMASRIPFSA 318

BLAST of Sed0003404 vs. NCBI nr
Match: XP_022938497.1 (pectinesterase 31 [Cucurbita moschata])

HSP 1 Score: 634.0 bits (1634), Expect = 6.8e-178
Identity = 292/318 (91.82%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   MAAPPRVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITF 60
           MAAPPRV+TVA DG GDF TVQEAIDAVPFSNTCRTVIR++PGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLCPETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL PETT+LTWDNTATKI+HHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+GKQYLKDCYIEGSVDFIFGNSTALLE CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLERCH 180

Query: 181 VHCKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDA 240
           VHCKS GFITAQSRKSSQETTGYVFLRCVITGS   S+ HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 VHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFIDPD 300
           CIKPAGWDNWGK ENERTACFYEYKCFGPGS S  RVCWAREL+DEEADEF+LHQFIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 IERRWLCQRMALRIPFSA 319
           +ER WLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

BLAST of Sed0003404 vs. NCBI nr
Match: KAG6578565.1 (Pectinesterase 31, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 633.6 bits (1633), Expect = 8.9e-178
Identity = 291/318 (91.51%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   MAAPPRVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITF 60
           MAAPPRV+TVA DG GDF TVQEAIDAVPFSNTCRTVIR++PGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLCPETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL PETT+LTWDNTATKI+HHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+GKQYLKDCYIEGSVDFIFGNSTALLE CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLERCH 180

Query: 181 VHCKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDA 240
           +HCKS GFITAQSRKSSQETTGYVFLRCVITGS   S+ HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 IHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFIDPD 300
           CIKPAGWDNWGK ENERTACFYEYKCFGPGS S  RVCWAREL+DEEADEF+LHQFIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 IERRWLCQRMALRIPFSA 319
           +ER WLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

BLAST of Sed0003404 vs. NCBI nr
Match: XP_004141450.1 (pectinesterase 31 [Cucumis sativus] >KGN52482.1 hypothetical protein Csa_007777 [Cucumis sativus])

HSP 1 Score: 633.3 bits (1632), Expect = 1.2e-177
Identity = 288/318 (90.57%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MAAPPRVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITF 60
           MAA PRV+TVAHDG  DF TVQEAIDAVPFSNTCRT+IR++PGIY+QPLYVPKTKNFITF
Sbjct: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60

Query: 61  AGLCPETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL PETTILTWDNTATKINHHQA+RVIGTGTFGCGSTIVEGEDF+AENITFENSSPQGS
Sbjct: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+G+QYLKDCYIEGSVDFIFGNSTALLE+CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 VHCKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDA 240
           VHCKS+GFITAQSRKSSQETTGYVFLRCVITGSGE S+ HLGRPWGPFARVVFAYT+MD 
Sbjct: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240

Query: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFIDPD 300
           CIKPAGWDNWGK ENERTACFYEYKCFGPGS S KRVCWAREL+DEEADEF+LH+FIDPD
Sbjct: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300

Query: 301 IERRWLCQRMALRIPFSA 319
           ++R WLCQRMALRIPFSA
Sbjct: 301 VDRPWLCQRMALRIPFSA 318

BLAST of Sed0003404 vs. NCBI nr
Match: XP_038891224.1 (pectinesterase 31 [Benincasa hispida])

HSP 1 Score: 632.5 bits (1630), Expect = 2.0e-177
Identity = 288/318 (90.57%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MAAPPRVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITF 60
           MAAPPR++TVAHDG  DF TVQEAIDAVPFSNTCRT+IR++PGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRLLTVAHDGCADFRTVQEAIDAVPFSNTCRTIIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLCPETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL PETTILTWDNTATKI+HHQASRVIGTGTFGCG+TIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTILTWDNTATKISHHQASRVIGTGTFGCGTTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHG+QYLKDCYIEGSVDFIFGNSTALLE+CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 VHCKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDA 240
           +HCKS+GFITAQSRKSSQETTGYVFLRCVITGSGE S+ +LGRPWGPFARVVFAYTYMDA
Sbjct: 181 IHCKSQGFITAQSRKSSQETTGYVFLRCVITGSGETSYVNLGRPWGPFARVVFAYTYMDA 240

Query: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFIDPD 300
           CIKP GWDNWGK ENERTACFYEYKCFGPGS S KRVCWAREL+DEEADEF+LH+FIDPD
Sbjct: 241 CIKPTGWDNWGKTENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFILHRFIDPD 300

Query: 301 IERRWLCQRMALRIPFSA 319
           IE+ WLC RMALR+PFSA
Sbjct: 301 IEKPWLCLRMALRLPFSA 318

BLAST of Sed0003404 vs. ExPASy Swiss-Prot
Match: Q9LVQ0 (Pectinesterase 31 OS=Arabidopsis thaliana OX=3702 GN=PME31 PE=1 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 2.0e-156
Identity = 244/316 (77.22%), Postives = 283/316 (89.56%), Query Frame = 0

Query: 3   APPRVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITFAG 62
           A  R++ V+ DG GD+ +VQ+AID+VP  NTCRTVIR++PGIYRQP+YVPK KNFITFAG
Sbjct: 2   ATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYRQPVYVPKRKNFITFAG 61

Query: 63  LCPETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQ 122
           + PE T+LTW+NTA+KI HHQASRVIGTGTFGCGS IVEGEDFIAENITFENS+P+GSGQ
Sbjct: 62  ISPEITVLTWNNTASKIEHHQASRVIGTGTFGCGSVIVEGEDFIAENITFENSAPEGSGQ 121

Query: 123 AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCHVH 182
           AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLE+CH+H
Sbjct: 122 AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLEHCHIH 181

Query: 183 CKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDACI 242
           CKS+GFITAQSRKSSQE+TGYVFLRCVITG+G++ + +LGRPWGPF RVV AYTYMDACI
Sbjct: 182 CKSQGFITAQSRKSSQESTGYVFLRCVITGNGQSGYMYLGRPWGPFGRVVLAYTYMDACI 241

Query: 243 KPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFIDPDIE 302
           +  GW NWG  ENER+ACFYEY+CFGPGS S++RV W+RELMD+EA  F+ H F+DP+ +
Sbjct: 242 RNVGWHNWGNAENERSACFYEYRCFGPGSCSSERVPWSRELMDDEAGHFVHHSFVDPEQD 301

Query: 303 RRWLCQRMALRIPFSA 319
           R WLC RM ++ P+SA
Sbjct: 302 RPWLCLRMGVKTPYSA 317

BLAST of Sed0003404 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.6e-73
Identity = 143/290 (49.31%), Postives = 180/290 (62.07%), Query Frame = 0

Query: 16  GDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITFAGLCPETTILTWDNT 75
           GDFT +Q+AID++P  N  R VI++  G+Y++ + +P  K FIT  G   E T + W +T
Sbjct: 94  GDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDT 153

Query: 76  ATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QGSGQAVAIRVTA 135
           A   +    S+    GT+   S  V    F+A+NITF N++P         QAVA+RV+A
Sbjct: 154 AQTPD----SKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 213

Query: 136 DRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCHVH--CKSRGF 195
           D  AF+ CR LG QDTLY H G+ Y KDCYIEGSVDFIFGN+ +L E CHVH      G 
Sbjct: 214 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 273

Query: 196 ITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDACIKPAGWD 255
           +TAQ R S  E TG+ F++C +TG+G     +LGR WGPF+RVVFAYTYMD  I P GW 
Sbjct: 274 VTAQGRSSVLEDTGFSFVKCKVTGTGV---LYLGRAWGPFSRVVFAYTYMDNIILPRGWY 333

Query: 256 NWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFID 299
           NWG    E T  + +YKC G G+    RV WAREL DEEA  FL   FID
Sbjct: 334 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFID 376

BLAST of Sed0003404 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 5.4e-61
Identity = 120/295 (40.68%), Postives = 170/295 (57.63%), Query Frame = 0

Query: 7   VITVAHDGGGDFTTVQEAIDAVP--FSNTCRTVIRIAPGIYRQPLYVPKTKNFITFAGLC 66
           +I V   G GDF+ +QEAI+++P   +N+    I + PGIYR+ + +P  K +IT +G  
Sbjct: 50  LIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQ 109

Query: 67  PETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQAV 126
              T L W +              G       +  +   DF+   +T +N     +G+AV
Sbjct: 110 ASNTFLIWSD--------------GEDILESPTLTIFASDFVCRFLTIQNKFGT-AGRAV 169

Query: 127 AIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCHVHCK 186
           A+RV AD+ AFY C    +QDTL   +G  Y K+CYIEG+ DFI G++++L E CH+H  
Sbjct: 170 ALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSL 229

Query: 187 S--RGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDACI 246
           S   G ITAQ R S+ E +G+ FL C +TGSG    T LGRPWG ++RVVFAY++    +
Sbjct: 230 SPNNGSITAQMRTSATEKSGFTFLGCKLTGSGS---TFLGRPWGAYSRVVFAYSFFSNVV 289

Query: 247 KPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFI 298
            P GW+ WG    E T  + EYKC+GPG+   +RV W+++L DEEA  FL   FI
Sbjct: 290 APQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFI 326

BLAST of Sed0003404 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.5e-60
Identity = 121/314 (38.54%), Postives = 177/314 (56.37%), Query Frame = 0

Query: 6   RVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITFAGLCP 65
           +VITV+ +G   F +VQ+A+D++P +N     I+IAPG YR+ + VP TK +ITF G   
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 66  ETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QGS 125
           + T + W + A+ +  +         T+   S  V    F A NI+F N++P        
Sbjct: 119 DVTAIEWHDRASDLGANGQQ----LRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQG 178

Query: 126 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCH 185
            QAVA R++ D+  F  C F G QDTL    G+ Y K+CYIEGS+DFIFGN  ++ ++C 
Sbjct: 179 WQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCE 238

Query: 186 VH--CKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYM 245
           +H      G I A  R   +E TG+ F+ C +TG+G     ++GR  G ++R+V+AYTY 
Sbjct: 239 LHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGP---LYVGRAMGQYSRIVYAYTYF 298

Query: 246 DACIKPAGWDNWG-KIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFI 305
           DA +   GWD+W  K    +TA F  Y C+GPG+ +T+ V WAR L  E A  F+   F+
Sbjct: 299 DALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFV 358

Query: 306 DPDIERRWLCQRMA 312
           +    R W+  R A
Sbjct: 359 N---GRHWIAPRDA 362

BLAST of Sed0003404 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.1e-59
Identity = 122/312 (39.10%), Postives = 178/312 (57.05%), Query Frame = 0

Query: 7   VITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITFAGLCPE 66
           V+TV   G G+F+ VQ AID VP  ++ +T+I +  G YR+ + V + K  +   G   +
Sbjct: 90  VLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQ 149

Query: 67  TTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QGSG 126
            T + W++TA    +          T    S +V   +F A NI+F+N++P     +   
Sbjct: 150 NTSIEWNDTAKSAGN----------TADSFSFVVFAANFTAYNISFKNNAPEPDPGEADA 209

Query: 127 QAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCHV 186
           QAVA+R+  D+ AFY C F G QDTL    G+ + K+C+I+GS+DFIFGN  +L ++C +
Sbjct: 210 QAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTI 269

Query: 187 HCKSR-------GFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFA 246
           +  ++       G ITAQ R+S  E +G+ F+ C I GSGE     LGR WG +A VVF+
Sbjct: 270 NSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEIL---LGRAWGAYATVVFS 329

Query: 247 YTYMDACIKPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLH 306
            TYM   I P GW+NWG    E+T  F E+KC+GPG+   +RV + ++L D EA  F+  
Sbjct: 330 NTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDV 385

BLAST of Sed0003404 vs. ExPASy TrEMBL
Match: A0A6J1BZD5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111006628 PE=4 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 3.9e-179
Identity = 293/318 (92.14%), Postives = 305/318 (95.91%), Query Frame = 0

Query: 1   MAAPPRVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITF 60
           MAAPPRVITVAHDG GDF TVQEAIDAVPFSNTCRTVIR++PGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVITVAHDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLCPETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL PE+T+LTWDNTATKINHHQASR+IGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLSPESTVLTWDNTATKINHHQASRIIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCH 180
           GQAVAIRVTADRCAFYNC+FLGWQDTLYLH+GKQYLKDCYIEGSVDFIFGNSTALLE+CH
Sbjct: 121 GQAVAIRVTADRCAFYNCKFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 VHCKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDA 240
           VHCKS GFITAQSRKSSQETTGYVFLRCVITGSG  S+ HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 VHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGNSYIHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFIDPD 300
           CIKPAGWDNWGKIENERTACFYEYKCFGPGS   KRVCWAREL+DEE DEFLLHQFIDP+
Sbjct: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSCQMKRVCWARELVDEETDEFLLHQFIDPN 300

Query: 301 IERRWLCQRMALRIPFSA 319
           +ER WLCQRMA RIPFSA
Sbjct: 301 VERPWLCQRMASRIPFSA 318

BLAST of Sed0003404 vs. ExPASy TrEMBL
Match: A0A6J1FE78 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111444715 PE=4 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 3.3e-178
Identity = 292/318 (91.82%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   MAAPPRVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITF 60
           MAAPPRV+TVA DG GDF TVQEAIDAVPFSNTCRTVIR++PGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLCPETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL PETT+LTWDNTATKI+HHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+GKQYLKDCYIEGSVDFIFGNSTALLE CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLERCH 180

Query: 181 VHCKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDA 240
           VHCKS GFITAQSRKSSQETTGYVFLRCVITGS   S+ HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 VHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFIDPD 300
           CIKPAGWDNWGK ENERTACFYEYKCFGPGS S  RVCWAREL+DEEADEF+LHQFIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 IERRWLCQRMALRIPFSA 319
           +ER WLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

BLAST of Sed0003404 vs. ExPASy TrEMBL
Match: A0A0A0KVN5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G637720 PE=4 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 5.6e-178
Identity = 288/318 (90.57%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MAAPPRVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITF 60
           MAA PRV+TVAHDG  DF TVQEAIDAVPFSNTCRT+IR++PGIY+QPLYVPKTKNFITF
Sbjct: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60

Query: 61  AGLCPETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL PETTILTWDNTATKINHHQA+RVIGTGTFGCGSTIVEGEDF+AENITFENSSPQGS
Sbjct: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+G+QYLKDCYIEGSVDFIFGNSTALLE+CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 VHCKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDA 240
           VHCKS+GFITAQSRKSSQETTGYVFLRCVITGSGE S+ HLGRPWGPFARVVFAYT+MD 
Sbjct: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240

Query: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFIDPD 300
           CIKPAGWDNWGK ENERTACFYEYKCFGPGS S KRVCWAREL+DEEADEF+LH+FIDPD
Sbjct: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300

Query: 301 IERRWLCQRMALRIPFSA 319
           ++R WLCQRMALRIPFSA
Sbjct: 301 VDRPWLCQRMALRIPFSA 318

BLAST of Sed0003404 vs. ExPASy TrEMBL
Match: A0A6J1JZ39 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111489575 PE=4 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.3e-177
Identity = 290/318 (91.19%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   MAAPPRVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITF 60
           MAAPPRV+TVA DG GDF TVQEAIDAVPFSNTCRTVIR++PGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLCPETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL PETT+LTWDNTATKI+HHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+GKQYLKDCYIEGSVDFIFGNSTALLE CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLERCH 180

Query: 181 VHCKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDA 240
           +HCKS GFITAQSRKSSQETTGYVFLRCVITGS  +S+ HLGRPWGPFARVVFAYTYMDA
Sbjct: 181 IHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGSSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFIDPD 300
           CIKPAGWDNWGK ENERTACFYEYKCFGPGS    RVCWAREL+DEEADEF+LHQFIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCPMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 IERRWLCQRMALRIPFSA 319
           +ER WLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

BLAST of Sed0003404 vs. ExPASy TrEMBL
Match: A0A5A7TBP8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002680 PE=4 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 1.1e-176
Identity = 287/318 (90.25%), Postives = 304/318 (95.60%), Query Frame = 0

Query: 1   MAAPPRVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITF 60
           MAA PRV+TVAHDG  DF TVQEAIDAVPFSNTCRTVIR++PGIY+QPLYVPKTKNFITF
Sbjct: 1   MAAQPRVLTVAHDGCADFRTVQEAIDAVPFSNTCRTVIRVSPGIYKQPLYVPKTKNFITF 60

Query: 61  AGLCPETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL PETTILTWDNTAT+I HHQA+RVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTILTWDNTATEIKHHQAARVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+G+QYLKDCYIEGSVDFIFGNSTALLE+CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 VHCKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDA 240
           VHCKS+GFITAQSRKSSQETTGYVFLRCVITGSGE S+ HLGRPWGPFARVVFAYT+MD 
Sbjct: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240

Query: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFIDPD 300
           CIKP GWDNWGK ENERTACFYEYKCFGPGS S KRVCWAREL+DEEADEF+LH+FIDPD
Sbjct: 241 CIKPTGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300

Query: 301 IERRWLCQRMALRIPFSA 319
           ++R WLCQRMALRIPFSA
Sbjct: 301 VDRPWLCQRMALRIPFSA 318

BLAST of Sed0003404 vs. TAIR 10
Match: AT3G29090.1 (pectin methylesterase 31 )

HSP 1 Score: 553.1 bits (1424), Expect = 1.4e-157
Identity = 244/316 (77.22%), Postives = 283/316 (89.56%), Query Frame = 0

Query: 3   APPRVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITFAG 62
           A  R++ V+ DG GD+ +VQ+AID+VP  NTCRTVIR++PGIYRQP+YVPK KNFITFAG
Sbjct: 2   ATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYRQPVYVPKRKNFITFAG 61

Query: 63  LCPETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQ 122
           + PE T+LTW+NTA+KI HHQASRVIGTGTFGCGS IVEGEDFIAENITFENS+P+GSGQ
Sbjct: 62  ISPEITVLTWNNTASKIEHHQASRVIGTGTFGCGSVIVEGEDFIAENITFENSAPEGSGQ 121

Query: 123 AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCHVH 182
           AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLE+CH+H
Sbjct: 122 AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLEHCHIH 181

Query: 183 CKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDACI 242
           CKS+GFITAQSRKSSQE+TGYVFLRCVITG+G++ + +LGRPWGPF RVV AYTYMDACI
Sbjct: 182 CKSQGFITAQSRKSSQESTGYVFLRCVITGNGQSGYMYLGRPWGPFGRVVLAYTYMDACI 241

Query: 243 KPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFIDPDIE 302
           +  GW NWG  ENER+ACFYEY+CFGPGS S++RV W+RELMD+EA  F+ H F+DP+ +
Sbjct: 242 RNVGWHNWGNAENERSACFYEYRCFGPGSCSSERVPWSRELMDDEAGHFVHHSFVDPEQD 301

Query: 303 RRWLCQRMALRIPFSA 319
           R WLC RM ++ P+SA
Sbjct: 302 RPWLCLRMGVKTPYSA 317

BLAST of Sed0003404 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.2e-74
Identity = 143/290 (49.31%), Postives = 180/290 (62.07%), Query Frame = 0

Query: 16  GDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITFAGLCPETTILTWDNT 75
           GDFT +Q+AID++P  N  R VI++  G+Y++ + +P  K FIT  G   E T + W +T
Sbjct: 94  GDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDT 153

Query: 76  ATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QGSGQAVAIRVTA 135
           A   +    S+    GT+   S  V    F+A+NITF N++P         QAVA+RV+A
Sbjct: 154 AQTPD----SKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 213

Query: 136 DRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCHVH--CKSRGF 195
           D  AF+ CR LG QDTLY H G+ Y KDCYIEGSVDFIFGN+ +L E CHVH      G 
Sbjct: 214 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 273

Query: 196 ITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDACIKPAGWD 255
           +TAQ R S  E TG+ F++C +TG+G     +LGR WGPF+RVVFAYTYMD  I P GW 
Sbjct: 274 VTAQGRSSVLEDTGFSFVKCKVTGTGV---LYLGRAWGPFSRVVFAYTYMDNIILPRGWY 333

Query: 256 NWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFID 299
           NWG    E T  + +YKC G G+    RV WAREL DEEA  FL   FID
Sbjct: 334 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFID 376

BLAST of Sed0003404 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 236.1 bits (601), Expect = 3.9e-62
Identity = 120/295 (40.68%), Postives = 170/295 (57.63%), Query Frame = 0

Query: 7   VITVAHDGGGDFTTVQEAIDAVP--FSNTCRTVIRIAPGIYRQPLYVPKTKNFITFAGLC 66
           +I V   G GDF+ +QEAI+++P   +N+    I + PGIYR+ + +P  K +IT +G  
Sbjct: 50  LIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQ 109

Query: 67  PETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQAV 126
              T L W +              G       +  +   DF+   +T +N     +G+AV
Sbjct: 110 ASNTFLIWSD--------------GEDILESPTLTIFASDFVCRFLTIQNKFGT-AGRAV 169

Query: 127 AIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCHVHCK 186
           A+RV AD+ AFY C    +QDTL   +G  Y K+CYIEG+ DFI G++++L E CH+H  
Sbjct: 170 ALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSL 229

Query: 187 S--RGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYMDACI 246
           S   G ITAQ R S+ E +G+ FL C +TGSG    T LGRPWG ++RVVFAY++    +
Sbjct: 230 SPNNGSITAQMRTSATEKSGFTFLGCKLTGSGS---TFLGRPWGAYSRVVFAYSFFSNVV 289

Query: 247 KPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFI 298
            P GW+ WG    E T  + EYKC+GPG+   +RV W+++L DEEA  FL   FI
Sbjct: 290 APQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFI 326

BLAST of Sed0003404 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.5e-61
Identity = 121/314 (38.54%), Postives = 177/314 (56.37%), Query Frame = 0

Query: 6   RVITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITFAGLCP 65
           +VITV+ +G   F +VQ+A+D++P +N     I+IAPG YR+ + VP TK +ITF G   
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 66  ETTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QGS 125
           + T + W + A+ +  +         T+   S  V    F A NI+F N++P        
Sbjct: 119 DVTAIEWHDRASDLGANGQQ----LRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQG 178

Query: 126 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCH 185
            QAVA R++ D+  F  C F G QDTL    G+ Y K+CYIEGS+DFIFGN  ++ ++C 
Sbjct: 179 WQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCE 238

Query: 186 VH--CKSRGFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFAYTYM 245
           +H      G I A  R   +E TG+ F+ C +TG+G     ++GR  G ++R+V+AYTY 
Sbjct: 239 LHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGP---LYVGRAMGQYSRIVYAYTYF 298

Query: 246 DACIKPAGWDNWG-KIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLHQFI 305
           DA +   GWD+W  K    +TA F  Y C+GPG+ +T+ V WAR L  E A  F+   F+
Sbjct: 299 DALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFV 358

Query: 306 DPDIERRWLCQRMA 312
           +    R W+  R A
Sbjct: 359 N---GRHWIAPRDA 362

BLAST of Sed0003404 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 229.6 bits (584), Expect = 3.6e-60
Identity = 122/312 (39.10%), Postives = 178/312 (57.05%), Query Frame = 0

Query: 7   VITVAHDGGGDFTTVQEAIDAVPFSNTCRTVIRIAPGIYRQPLYVPKTKNFITFAGLCPE 66
           V+TV   G G+F+ VQ AID VP  ++ +T+I +  G YR+ + V + K  +   G   +
Sbjct: 90  VLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQ 149

Query: 67  TTILTWDNTATKINHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QGSG 126
            T + W++TA    +          T    S +V   +F A NI+F+N++P     +   
Sbjct: 150 NTSIEWNDTAKSAGN----------TADSFSFVVFAANFTAYNISFKNNAPEPDPGEADA 209

Query: 127 QAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLENCHV 186
           QAVA+R+  D+ AFY C F G QDTL    G+ + K+C+I+GS+DFIFGN  +L ++C +
Sbjct: 210 QAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTI 269

Query: 187 HCKSR-------GFITAQSRKSSQETTGYVFLRCVITGSGEASHTHLGRPWGPFARVVFA 246
           +  ++       G ITAQ R+S  E +G+ F+ C I GSGE     LGR WG +A VVF+
Sbjct: 270 NSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEIL---LGRAWGAYATVVFS 329

Query: 247 YTYMDACIKPAGWDNWGKIENERTACFYEYKCFGPGSFSTKRVCWARELMDEEADEFLLH 306
            TYM   I P GW+NWG    E+T  F E+KC+GPG+   +RV + ++L D EA  F+  
Sbjct: 330 NTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDV 385

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134347.18.1e-17992.14pectinesterase 31 [Momordica charantia][more]
XP_022938497.16.8e-17891.82pectinesterase 31 [Cucurbita moschata][more]
KAG6578565.18.9e-17891.51Pectinesterase 31, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_004141450.11.2e-17790.57pectinesterase 31 [Cucumis sativus] >KGN52482.1 hypothetical protein Csa_007777 ... [more]
XP_038891224.12.0e-17790.57pectinesterase 31 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9LVQ02.0e-15677.22Pectinesterase 31 OS=Arabidopsis thaliana OX=3702 GN=PME31 PE=1 SV=1[more]
Q8VYZ31.6e-7349.31Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
Q9SIJ95.4e-6140.68Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1[more]
Q8LPF33.5e-6038.54Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1[more]
Q9ZQA35.1e-5939.10Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BZD53.9e-17992.14Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111006628 PE=4 SV=1[more]
A0A6J1FE783.3e-17891.82Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111444715 PE=4 SV=1[more]
A0A0A0KVN55.6e-17890.57Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G637720 PE=4 SV=1[more]
A0A6J1JZ391.3e-17791.19Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111489575 PE=4 SV=1[more]
A0A5A7TBP81.1e-17690.25Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00268... [more]
Match NameE-valueIdentityDescription
AT3G29090.11.4e-15777.22pectin methylesterase 31 [more]
AT5G19730.11.2e-7449.31Pectin lyase-like superfamily protein [more]
AT2G21610.13.9e-6240.68pectinesterase 11 [more]
AT5G47500.12.5e-6138.54Pectin lyase-like superfamily protein [more]
AT2G36710.13.6e-6039.10Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 4..311
e-value: 1.5E-109
score: 368.3
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 8..298
e-value: 9.4E-74
score: 247.8
NoneNo IPR availablePANTHERPTHR31321:SF71PECTINESTERASEcoord: 6..317
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 6..317
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 161..170
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 2..301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0003404.1Sed0003404.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0050829 defense response to Gram-negative bacterium
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity