Sed0003135 (gene) Chayote v1

BLAST of Sed0003135 vs. NCBI nr
Match: XP_022927385.1 (putative E3 ubiquitin-protein ligase RING1a [Cucurbita moschata] >XP_023519096.1 putative E3 ubiquitin-protein ligase RING1a [Cucurbita pepo subsp. pepo])

HSP 1 Score: 619.0 bits (1595), Expect = 3.5e-173
Identity = 346/437 (79.18%), Postives = 374/437 (85.58%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLGHGFS----KDEDADESDRSASSSSNEEKDEYVFYFATFRVL 60
           MPA+KRAPESSEDD LKL HG S    +D DADESDRS SSSSN EKDE++        L
Sbjct: 1   MPAKKRAPESSEDDSLKLDHGCSAARPEDGDADESDRSTSSSSNGEKDEFIVV-----KL 60

Query: 61  FFVFFSIDYYVALNRIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 120
             +   +   + L  IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR
Sbjct: 61  SDIRKEVQCPICLG-IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 120

Query: 121 DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRPPSV 180
           DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEA+SKKRPPSV
Sbjct: 121 DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSV 180

Query: 181 RRLRGNNYRSNHSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFR 240
           RRLRGNNYR++ S+GKRSYRNDAEF ASDDNEDPTAEDGVKDSSSDEQM+RSERRSKGFR
Sbjct: 181 RRLRGNNYRNSQSKGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRSKGFR 240

Query: 241 KQGAAKFSQSHFAACSDGAGEDNDSDRVHGETM-GSSRLVSSSKRLVWGKGGMRSHTRHG 300
           KQGAAKFSQ  FAACSDGAGEDNDSD+V+ E +  SSRLV +S+RLVWGKGGMRSHTRHG
Sbjct: 241 KQGAAKFSQPPFAACSDGAGEDNDSDQVNREILSASSRLVGNSERLVWGKGGMRSHTRHG 300

Query: 301 SMTVANNKNVKNNRLSALADYFRNSNNNA-EREVHLLLLSYDQRIIPSLQLPYLCCQQAL 360
            M+V ++KNVKNNR+S L D  R S+NNA EREV LLL SYDQRIIPSLQLPY+CC+   
Sbjct: 301 CMSVGSSKNVKNNRISTLVDSLRTSDNNAEEREVCLLLFSYDQRIIPSLQLPYICCRPTS 360

Query: 361 SVRHLCRYVAHHTGFQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRSTKDNFQVLSE 420
           SVRHLC+YVA  TGFQADEIDM LI ELN KIE  TSID LVSKSLIP   +D+FQVL E
Sbjct: 361 SVRHLCQYVAGQTGFQADEIDMFLIQELNLKIEPSTSIDVLVSKSLIPHPIEDHFQVLRE 420

Query: 421 QQTLAEIKTHCSTRGQL 432
           QQTLAE+KTHCSTRGQL
Sbjct: 421 QQTLAELKTHCSTRGQL 431

BLAST of Sed0003135 vs. NCBI nr
Match: XP_023001718.1 (putative E3 ubiquitin-protein ligase RING1a [Cucurbita maxima])

HSP 1 Score: 617.8 bits (1592), Expect = 7.8e-173
Identity = 346/437 (79.18%), Postives = 374/437 (85.58%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLGHGFS----KDEDADESDRSASSSSNEEKDEYVFYFATFRVL 60
           MPA+KRAPESSEDD LKL HGFS    +D DADESDRS SSSSN EKDE++        L
Sbjct: 1   MPAKKRAPESSEDDSLKLDHGFSAARPEDGDADESDRSTSSSSNGEKDEFIVV-----KL 60

Query: 61  FFVFFSIDYYVALNRIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 120
             +   +   + L  IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR
Sbjct: 61  SDIRKEVQCPICLG-IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 120

Query: 121 DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRPPSV 180
           DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEA+SKKRPPSV
Sbjct: 121 DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSV 180

Query: 181 RRLRGNNYRSNHSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFR 240
           RRLRGNNYR++ S+GKRSYRNDAEF ASDDNEDPTAEDGVKDSSSDEQM+RSERRSKGFR
Sbjct: 181 RRLRGNNYRNSQSKGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRSKGFR 240

Query: 241 KQGAAKFSQSHFAACSDGAGEDNDSDRVHGETM-GSSRLVSSSKRLVWGKGGMRSHTRHG 300
           KQGAAKFSQ  FAA SDGAGEDNDSD+V+ E +  SSRLV +S+RLVWGKGGMRSHTRHG
Sbjct: 241 KQGAAKFSQPPFAAYSDGAGEDNDSDQVNREILSASSRLVGNSERLVWGKGGMRSHTRHG 300

Query: 301 SMTVANNKNVKNNRLSALADYFRNSNNNA-EREVHLLLLSYDQRIIPSLQLPYLCCQQAL 360
            M+V ++KNVKNNR+S L D  R S+NNA EREV LLL SYDQRIIPSLQLPY+CC+   
Sbjct: 301 CMSVGSSKNVKNNRISTLVDSLRTSDNNAEEREVCLLLFSYDQRIIPSLQLPYICCRPTS 360

Query: 361 SVRHLCRYVAHHTGFQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRSTKDNFQVLSE 420
           SVRHLC+YVA  TGFQADEIDM LI ELN KIE  TSID LVSKSLIP   +D+FQVL E
Sbjct: 361 SVRHLCQYVAGQTGFQADEIDMFLIQELNLKIEPSTSIDVLVSKSLIPHPIEDHFQVLRE 420

Query: 421 QQTLAEIKTHCSTRGQL 432
           QQTLAE+KTHCSTRGQL
Sbjct: 421 QQTLAELKTHCSTRGQL 431

BLAST of Sed0003135 vs. NCBI nr
Match: KAG6583983.1 (putative E3 ubiquitin-protein ligase RING1a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 616.3 bits (1588), Expect = 2.3e-172
Identity = 345/437 (78.95%), Postives = 373/437 (85.35%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLGHGFS----KDEDADESDRSASSSSNEEKDEYVFYFATFRVL 60
           MPA+KRAPESSEDD LKL HG S    +D DADESDRS SSSSN EKDE++        L
Sbjct: 1   MPAKKRAPESSEDDSLKLEHGCSAARPEDGDADESDRSTSSSSNGEKDEFIVV-----KL 60

Query: 61  FFVFFSIDYYVALNRIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 120
             +   +   + L  IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR
Sbjct: 61  SDIRKEVQCPICLG-IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 120

Query: 121 DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRPPSV 180
           DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEA+SKKRPPSV
Sbjct: 121 DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSV 180

Query: 181 RRLRGNNYRSNHSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFR 240
           RRLRGNNYR++ S+GKRSYRNDAEF ASDDNEDPTAEDGVKDSSSDEQM+RSERRSKGFR
Sbjct: 181 RRLRGNNYRNSQSKGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRSKGFR 240

Query: 241 KQGAAKFSQSHFAACSDGAGEDNDSDRVHGETM-GSSRLVSSSKRLVWGKGGMRSHTRHG 300
           KQGAAKFSQ  FAACSDGAGEDNDSD+V+ E +  SS LV +S+RLVWGKGGMRSHTRHG
Sbjct: 241 KQGAAKFSQPPFAACSDGAGEDNDSDQVNREILSASSMLVGNSERLVWGKGGMRSHTRHG 300

Query: 301 SMTVANNKNVKNNRLSALADYFRNSNNNA-EREVHLLLLSYDQRIIPSLQLPYLCCQQAL 360
            M+V ++KNVKNNR+S L D  R S+NNA EREV LLL SYDQRIIPSLQLPY+CC+   
Sbjct: 301 CMSVGSSKNVKNNRISTLVDSLRTSDNNAEEREVCLLLFSYDQRIIPSLQLPYICCRPTS 360

Query: 361 SVRHLCRYVAHHTGFQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRSTKDNFQVLSE 420
           SVRHLC+YVA  TGFQADEIDM LI ELN KIE  TSID LVSKSLIP   +D+FQVL E
Sbjct: 361 SVRHLCQYVAGQTGFQADEIDMFLIQELNLKIEPSTSIDVLVSKSLIPHPIEDHFQVLRE 420

Query: 421 QQTLAEIKTHCSTRGQL 432
           QQTLAE+KTHCSTRGQL
Sbjct: 421 QQTLAELKTHCSTRGQL 431

BLAST of Sed0003135 vs. NCBI nr
Match: XP_022139814.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Momordica charantia])

HSP 1 Score: 607.1 bits (1564), Expect = 1.4e-169
Identity = 342/440 (77.73%), Postives = 375/440 (85.23%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLGHGFS----KDEDADESDRSASSSSNEEKDEYVFYFATFRVL 60
           MPAQKRAPESSEDDPLK  HG S    +D D+++SDRS SSSSNEEKDE++        L
Sbjct: 1   MPAQKRAPESSEDDPLKRDHGCSSVHPEDGDSEQSDRSPSSSSNEEKDEFIVV-----KL 60

Query: 61  FFVFFSIDYYVALNRIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 120
             +   +   + L  IIRKTRTVMECLHRFCRECIDKSMR+GN ECPACRTHCASRRSLR
Sbjct: 61  SDIRKEVQCPICLG-IIRKTRTVMECLHRFCRECIDKSMRMGNKECPACRTHCASRRSLR 120

Query: 121 DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRPPSV 180
           DDPNYDSLI+ALYPDIEKYEEDELAFREEER+RNKQIQASIAQTL RQSEA+SKKRPPSV
Sbjct: 121 DDPNYDSLISALYPDIEKYEEDELAFREEERVRNKQIQASIAQTLHRQSEAVSKKRPPSV 180

Query: 181 RRLRG-NNYRSNHSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGF 240
           RRLRG NN+R+NHSRGKRSYRNDAEF  SDD EDPTAEDG KDSSSDEQ +R ERR+KGF
Sbjct: 181 RRLRGNNNHRNNHSRGKRSYRNDAEFPTSDDIEDPTAEDGGKDSSSDEQTERYERRTKGF 240

Query: 241 RKQGAAKFSQSH-FAACSDGAGEDNDSDRVHGETMG--SSRLVSSSKRLVWGKGGMRSHT 300
           R QGAAKFSQ    AACSDGAGEDNDS+ V+ E  G  SSR V SS+RLVWGKGGMRSHT
Sbjct: 241 RTQGAAKFSQPPCLAACSDGAGEDNDSE-VNREAPGAASSRHVGSSERLVWGKGGMRSHT 300

Query: 301 RHGSMTVANNKNVKNNRLSALADYFRNSNNNA-EREVHLLLLSYDQRIIPSLQLPYLCCQ 360
           RHGS+++ N+KNVKNNRLSALADY RNS++N+ EREVHLLL SYDQRIIPSLQLPY+CC+
Sbjct: 301 RHGSLSIGNSKNVKNNRLSALADYLRNSDDNSEEREVHLLLFSYDQRIIPSLQLPYVCCR 360

Query: 361 QALSVRHLCRYVAHHTGFQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRSTKDNFQV 420
            A SVRHLC+YVAH TGFQADEIDMLLI EL+PKIE  TSID LVSKSLIP  T+DNFQV
Sbjct: 361 PASSVRHLCQYVAHQTGFQADEIDMLLIQELHPKIEPSTSIDVLVSKSLIPHPTEDNFQV 420

Query: 421 LSEQQTLAEIKTHCSTRGQL 432
           L EQQTL E+KTHCSTRGQL
Sbjct: 421 LREQQTLGELKTHCSTRGQL 433

BLAST of Sed0003135 vs. NCBI nr
Match: KAG7019606.1 (putative E3 ubiquitin-protein ligase RING1a [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 600.9 bits (1548), Expect = 9.8e-168
Identity = 348/482 (72.20%), Postives = 377/482 (78.22%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLGHGFS----KDEDADESDRSASSSSNEEKDEYVFYFATF--- 60
           MPA+KRAPESSEDD LKL HG S    +D DADESDRS SSSSN EKDEYV YF TF   
Sbjct: 1   MPAKKRAPESSEDDSLKLDHGCSAARPEDGDADESDRSTSSSSNGEKDEYVLYFTTFARS 60

Query: 61  -----------RVL-----------------------FFVFFSIDYYVALN--------- 120
                      R+L                        +++  I   V L+         
Sbjct: 61  HLCFPSSNALGRILRKVGGVGFANFLGTATAAADSPNKYMYCLIFIVVKLSDIRKEVQCP 120

Query: 121 ---RIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAA 180
               IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAA
Sbjct: 121 ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAA 180

Query: 181 LYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRPPSVRRLRGNNYRSN 240
           LYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEA+SKKRPPSVRRLRGNNYR++
Sbjct: 181 LYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSVRRLRGNNYRNS 240

Query: 241 HSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFRKQGAAKFSQSH 300
            S+GKRSYRNDAEF ASDDNEDPTAEDGVKDSSSDEQM++SERRSKGFRKQGAAKFSQ  
Sbjct: 241 QSKGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMEQSERRSKGFRKQGAAKFSQPP 300

Query: 301 FAACSDGAGEDNDSDRVHGETM-GSSRLVSSSKRLVWGKGGMRSHTRHGSMTVANNKNVK 360
           FAACSDGAGEDNDSD+V+ E +  SSRLV +S+RLVWGKGGMRSHTRHG M+V ++KNVK
Sbjct: 301 FAACSDGAGEDNDSDQVNREILSASSRLVGNSERLVWGKGGMRSHTRHGCMSVGSSKNVK 360

Query: 361 NNRLSALADYFRNSNNNA-EREVHLLLLSYDQRIIPSLQLPYLCCQQALSVRHLCRYVAH 420
           NNR+S L D  R S+NNA EREV LLL SYDQRIIPSLQLPY+CC+   SVRHLC+YVA 
Sbjct: 361 NNRISTLVDSLRTSDNNAEEREVCLLLFSYDQRIIPSLQLPYICCRPTSSVRHLCQYVAG 420

Query: 421 HTGFQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRSTKDNFQVLSEQQTLAEIKTHC 428
            TGFQADEIDM LI ELN KIE  TSID LVSKSLIP   +D+FQVL EQQTLAE+KTH 
Sbjct: 421 QTGFQADEIDMFLIQELNLKIEPSTSIDVLVSKSLIPHPIEDHFQVLREQQTLAELKTHW 480

BLAST of Sed0003135 vs. ExPASy Swiss-Prot
Match: Q9FKW0 (Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=RING1A PE=1 SV=2)

HSP 1 Score: 241.1 bits (614), Expect = 2.6e-62
Identity = 175/417 (41.97%), Postives = 253/417 (60.67%), Query Frame = 0

Query: 24  KDEDADESDRSASSSSNEEKDEYVFYFATFRVLFFVFFSIDYYVALNRIIRKTRTVMECL 83
           ++E+ D  +RS SS S ++ +         R        +   + L  II+KTRTVMECL
Sbjct: 101 EEEEEDSKERSPSSISGDQSEFMEIDLGEIRK------DVQCPICLG-IIKKTRTVMECL 160

Query: 84  HRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAALYPDIEKYEEDELAFR 143
           HRFCRECIDKSMRLGNNECPACR HCASRRSLRDDP +D+LIAAL+ +I+ YEE+ELAF 
Sbjct: 161 HRFCRECIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFH 220

Query: 144 EEERIRNKQIQASIAQTLRRQSEALSKKRPPSVR--------RLRGNNYRSNHSRGKRSY 203
           E+E  RNKQIQASIAQ  +RQSEAL K+R             R+   + R  +SR     
Sbjct: 221 EDEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQ- 280

Query: 204 RNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFRKQGAAKFSQ---SHFAACS 263
           +N +E    DDN+D       KDSSSDE+   +E R K  RK+  ++ +Q   S  A  +
Sbjct: 281 QNASEAHEDDDNDDNNNRGRDKDSSSDER--GTEVRQKKRRKRSTSRSTQHPSSSGANKN 340

Query: 264 DGAGEDNDSDRVHGETMG-SSRLVSSSKRLVWGKGGMRSHTRHGSMTV-ANNKNVKNNRL 323
           +G   DND++ V+ +T G S  LV + + L WG+GG RS+TRHG+ T   ++K+V+N R+
Sbjct: 341 NGNCADNDTE-VYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARV 400

Query: 324 SALADYFRNS--NNNAEREVHLLLLSYDQRIIPSLQLPYLCCQQALSVRHLCRYVAHHTG 383
           + L +Y R+S   ++ E ++H+ L+S D + IP L  PYLCC+  L V+ L  +VA    
Sbjct: 401 NKLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIH 460

Query: 384 FQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRS---TKDNFQVLSEQQTLAEIK 423
            + +E+++L+      ++    +I++L   ++ P S   +KD    L + +TL+ +K
Sbjct: 461 LKTEEVELLVTRR---RVGEDAAIENL--PAVTPASAAASKDEMLSLEDNETLSRLK 501

BLAST of Sed0003135 vs. ExPASy Swiss-Prot
Match: Q0WX00 (Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=RING1B PE=1 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 2.1e-56
Identity = 170/426 (39.91%), Postives = 242/426 (56.81%), Query Frame = 0

Query: 12  EDDPLKLGHGFSKDEDA-----DESDRSASSSSNE-EKDEYVFYFATFRVLFFVFFSIDY 71
           E+D +K     ++DE+A     D  +RS SSS+ E  + EY+        L  +   +  
Sbjct: 49  EEDDVKRDQVEAEDEEALGEEEDSKERSQSSSAGELSESEYMVV-----DLADICKDVQC 108

Query: 72  YVALNRIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLI 131
            + L  IIRKTRTVMECLHRFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+D+LI
Sbjct: 109 SICLG-IIRKTRTVMECLHRFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALI 168

Query: 132 AALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRP--PSVRRLRGNN 191
           AAL+ +I+K+EE+EL FR+++  RNKQIQASIAQ  +RQS+AL K++        L  + 
Sbjct: 169 AALFKNIDKFEEEELNFRQDDEARNKQIQASIAQVSQRQSKALVKRKSVGKGTAILSRSR 228

Query: 192 YRSNHSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFRKQGAAKF 251
                SR +R+ RN  E   S+ N+D       KDSSSDE  +R        +K+ A + 
Sbjct: 229 RSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKDSSSDEPCERQR------KKRSATQP 288

Query: 252 SQSHFAACSDGAGEDNDSDRVHGETMGSSRLVSSSKRLVWGKGGMRSHTRHGSMTVANNK 311
           S S+     + AG   +        + S  LV +S+ + WG+GG RS+TR G+    NN+
Sbjct: 289 SSSNANNNDNCAGNGTEQTHQRDSRVISPVLVWNSELIAWGRGGTRSNTRQGN----NNQ 348

Query: 312 NV---KNNRLSALADYFRN-SNNNAEREVHLLLLSYDQRIIPSLQLPYLCCQQALSVRHL 371
                +N RL  L +Y  +   N+ E ++HL L+S D   + +L  PYLC +  L V+ L
Sbjct: 349 GAISKRNARLKRLVEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQL 408

Query: 372 CRYVAHHTGFQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRSTKDNFQVLSEQQTLA 426
             YVA H   +A+E+++L+  + +  I   TS + +              Q L + +T+A
Sbjct: 409 REYVARHLKLKAEEVELLVSKDGDTVIGNKTSTEKM--------------QSLQDDETVA 443

BLAST of Sed0003135 vs. ExPASy Swiss-Prot
Match: Q6MGB6 (E3 ubiquitin-protein ligase RING1 OS=Rattus norvegicus OX=10116 GN=Ring1 PE=2 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 3.8e-13
Identity = 69/208 (33.17%), Postives = 104/208 (50.00%), Query Frame = 0

Query: 72  IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAALYPD 131
           +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+RSLR DPN+D+LI+ +YP 
Sbjct: 54  MLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPS 113

Query: 132 IEKYE--EDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRPPSVRR-LRGNNYRSNH 191
            E+YE  +D +  R       + + +SI + LR Q    +  R   VRR + G++  +  
Sbjct: 114 REEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQ----AMHRAQRVRRPMPGSDQTTTM 173

Query: 192 SRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFRKQGAAKFSQSHF 251
           S G+                +   ED   DS+ D     + +R +G    G++  +    
Sbjct: 174 SGGE----------GEPGEGEGDGEDISSDSAPDSAPGPAPKRPRGGGAGGSSVGTGGGA 233

Query: 252 A--ACSDGAGEDNDSDRVHGETMGSSRL 275
           A  AC  GAG ++  DR  G T+G   L
Sbjct: 234 AGGACG-GAGSEDSGDR--GGTLGGGTL 244

BLAST of Sed0003135 vs. ExPASy Swiss-Prot
Match: O35730 (E3 ubiquitin-protein ligase RING1 OS=Mus musculus OX=10090 GN=Ring1 PE=1 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 5.0e-13
Identity = 69/208 (33.17%), Postives = 102/208 (49.04%), Query Frame = 0

Query: 72  IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAALYPD 131
           +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+RSLR DPN+D+LI+ +YP 
Sbjct: 54  MLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPS 113

Query: 132 IEKYE--EDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRPPSVRR-LRGNNYRSNH 191
            E+YE  +D +  R       + + +SI + LR Q    +  R   VRR + G++  +  
Sbjct: 114 REEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQ----AMHRAQRVRRPMPGSDQTATM 173

Query: 192 SRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFRKQGAAKFSQSHF 251
           S G+                +   ED   DS+ D     + +R +G    GA+       
Sbjct: 174 SGGE----------GEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRG-AGAGASSVGTGGG 233

Query: 252 AA--CSDGAGEDNDSDRVHGETMGSSRL 275
           AA     GAG ++  DR  G T+G   L
Sbjct: 234 AAGGACGGAGSEDSGDR--GGTLGGGTL 244

BLAST of Sed0003135 vs. ExPASy Swiss-Prot
Match: Q66J69 (E3 ubiquitin-protein ligase RING2-A OS=Xenopus laevis OX=8355 GN=rnf2-a PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 5.0e-13
Identity = 53/171 (30.99%), Postives = 87/171 (50.88%), Query Frame = 0

Query: 72  IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAALYPD 131
           +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+RSLR DPN+D+LI+ +YP 
Sbjct: 54  MLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPS 113

Query: 132 IEKYE-EDELAFREEERIRNKQ-IQASIAQTLRRQSEALSKKRPPSVRRLRGNNYRSNHS 191
            ++YE   +    +  R+ N+Q + +SI + L+ Q+                  +R+   
Sbjct: 114 RDEYEAHQDRVLAKLNRLHNQQALSSSIEEGLKMQA-----------------MHRAQRV 173

Query: 192 RGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFRKQGA 241
           R  +   ++  F   +DN D  +        S+++   S +RS+     GA
Sbjct: 174 RKHQHESDNTTFSGGEDNCDSRSHVSNPSVHSNQEAGPSRKRSRASEDSGA 207

BLAST of Sed0003135 vs. ExPASy TrEMBL
Match: A0A6J1ENS0 (putative E3 ubiquitin-protein ligase RING1a OS=Cucurbita moschata OX=3662 GN=LOC111434221 PE=4 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.7e-173
Identity = 346/437 (79.18%), Postives = 374/437 (85.58%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLGHGFS----KDEDADESDRSASSSSNEEKDEYVFYFATFRVL 60
           MPA+KRAPESSEDD LKL HG S    +D DADESDRS SSSSN EKDE++        L
Sbjct: 1   MPAKKRAPESSEDDSLKLDHGCSAARPEDGDADESDRSTSSSSNGEKDEFIVV-----KL 60

Query: 61  FFVFFSIDYYVALNRIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 120
             +   +   + L  IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR
Sbjct: 61  SDIRKEVQCPICLG-IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 120

Query: 121 DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRPPSV 180
           DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEA+SKKRPPSV
Sbjct: 121 DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSV 180

Query: 181 RRLRGNNYRSNHSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFR 240
           RRLRGNNYR++ S+GKRSYRNDAEF ASDDNEDPTAEDGVKDSSSDEQM+RSERRSKGFR
Sbjct: 181 RRLRGNNYRNSQSKGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRSKGFR 240

Query: 241 KQGAAKFSQSHFAACSDGAGEDNDSDRVHGETM-GSSRLVSSSKRLVWGKGGMRSHTRHG 300
           KQGAAKFSQ  FAACSDGAGEDNDSD+V+ E +  SSRLV +S+RLVWGKGGMRSHTRHG
Sbjct: 241 KQGAAKFSQPPFAACSDGAGEDNDSDQVNREILSASSRLVGNSERLVWGKGGMRSHTRHG 300

Query: 301 SMTVANNKNVKNNRLSALADYFRNSNNNA-EREVHLLLLSYDQRIIPSLQLPYLCCQQAL 360
            M+V ++KNVKNNR+S L D  R S+NNA EREV LLL SYDQRIIPSLQLPY+CC+   
Sbjct: 301 CMSVGSSKNVKNNRISTLVDSLRTSDNNAEEREVCLLLFSYDQRIIPSLQLPYICCRPTS 360

Query: 361 SVRHLCRYVAHHTGFQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRSTKDNFQVLSE 420
           SVRHLC+YVA  TGFQADEIDM LI ELN KIE  TSID LVSKSLIP   +D+FQVL E
Sbjct: 361 SVRHLCQYVAGQTGFQADEIDMFLIQELNLKIEPSTSIDVLVSKSLIPHPIEDHFQVLRE 420

Query: 421 QQTLAEIKTHCSTRGQL 432
           QQTLAE+KTHCSTRGQL
Sbjct: 421 QQTLAELKTHCSTRGQL 431

BLAST of Sed0003135 vs. ExPASy TrEMBL
Match: A0A6J1KRC5 (putative E3 ubiquitin-protein ligase RING1a OS=Cucurbita maxima OX=3661 GN=LOC111495770 PE=4 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 3.8e-173
Identity = 346/437 (79.18%), Postives = 374/437 (85.58%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLGHGFS----KDEDADESDRSASSSSNEEKDEYVFYFATFRVL 60
           MPA+KRAPESSEDD LKL HGFS    +D DADESDRS SSSSN EKDE++        L
Sbjct: 1   MPAKKRAPESSEDDSLKLDHGFSAARPEDGDADESDRSTSSSSNGEKDEFIVV-----KL 60

Query: 61  FFVFFSIDYYVALNRIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 120
             +   +   + L  IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR
Sbjct: 61  SDIRKEVQCPICLG-IIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 120

Query: 121 DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRPPSV 180
           DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEA+SKKRPPSV
Sbjct: 121 DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEAVSKKRPPSV 180

Query: 181 RRLRGNNYRSNHSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFR 240
           RRLRGNNYR++ S+GKRSYRNDAEF ASDDNEDPTAEDGVKDSSSDEQM+RSERRSKGFR
Sbjct: 181 RRLRGNNYRNSQSKGKRSYRNDAEFPASDDNEDPTAEDGVKDSSSDEQMERSERRSKGFR 240

Query: 241 KQGAAKFSQSHFAACSDGAGEDNDSDRVHGETM-GSSRLVSSSKRLVWGKGGMRSHTRHG 300
           KQGAAKFSQ  FAA SDGAGEDNDSD+V+ E +  SSRLV +S+RLVWGKGGMRSHTRHG
Sbjct: 241 KQGAAKFSQPPFAAYSDGAGEDNDSDQVNREILSASSRLVGNSERLVWGKGGMRSHTRHG 300

Query: 301 SMTVANNKNVKNNRLSALADYFRNSNNNA-EREVHLLLLSYDQRIIPSLQLPYLCCQQAL 360
            M+V ++KNVKNNR+S L D  R S+NNA EREV LLL SYDQRIIPSLQLPY+CC+   
Sbjct: 301 CMSVGSSKNVKNNRISTLVDSLRTSDNNAEEREVCLLLFSYDQRIIPSLQLPYICCRPTS 360

Query: 361 SVRHLCRYVAHHTGFQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRSTKDNFQVLSE 420
           SVRHLC+YVA  TGFQADEIDM LI ELN KIE  TSID LVSKSLIP   +D+FQVL E
Sbjct: 361 SVRHLCQYVAGQTGFQADEIDMFLIQELNLKIEPSTSIDVLVSKSLIPHPIEDHFQVLRE 420

Query: 421 QQTLAEIKTHCSTRGQL 432
           QQTLAE+KTHCSTRGQL
Sbjct: 421 QQTLAELKTHCSTRGQL 431

BLAST of Sed0003135 vs. ExPASy TrEMBL
Match: A0A6J1CE12 (putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010638 PE=4 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 6.6e-170
Identity = 342/440 (77.73%), Postives = 375/440 (85.23%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLGHGFS----KDEDADESDRSASSSSNEEKDEYVFYFATFRVL 60
           MPAQKRAPESSEDDPLK  HG S    +D D+++SDRS SSSSNEEKDE++        L
Sbjct: 1   MPAQKRAPESSEDDPLKRDHGCSSVHPEDGDSEQSDRSPSSSSNEEKDEFIVV-----KL 60

Query: 61  FFVFFSIDYYVALNRIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 120
             +   +   + L  IIRKTRTVMECLHRFCRECIDKSMR+GN ECPACRTHCASRRSLR
Sbjct: 61  SDIRKEVQCPICLG-IIRKTRTVMECLHRFCRECIDKSMRMGNKECPACRTHCASRRSLR 120

Query: 121 DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRPPSV 180
           DDPNYDSLI+ALYPDIEKYEEDELAFREEER+RNKQIQASIAQTL RQSEA+SKKRPPSV
Sbjct: 121 DDPNYDSLISALYPDIEKYEEDELAFREEERVRNKQIQASIAQTLHRQSEAVSKKRPPSV 180

Query: 181 RRLRG-NNYRSNHSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGF 240
           RRLRG NN+R+NHSRGKRSYRNDAEF  SDD EDPTAEDG KDSSSDEQ +R ERR+KGF
Sbjct: 181 RRLRGNNNHRNNHSRGKRSYRNDAEFPTSDDIEDPTAEDGGKDSSSDEQTERYERRTKGF 240

Query: 241 RKQGAAKFSQSH-FAACSDGAGEDNDSDRVHGETMG--SSRLVSSSKRLVWGKGGMRSHT 300
           R QGAAKFSQ    AACSDGAGEDNDS+ V+ E  G  SSR V SS+RLVWGKGGMRSHT
Sbjct: 241 RTQGAAKFSQPPCLAACSDGAGEDNDSE-VNREAPGAASSRHVGSSERLVWGKGGMRSHT 300

Query: 301 RHGSMTVANNKNVKNNRLSALADYFRNSNNNA-EREVHLLLLSYDQRIIPSLQLPYLCCQ 360
           RHGS+++ N+KNVKNNRLSALADY RNS++N+ EREVHLLL SYDQRIIPSLQLPY+CC+
Sbjct: 301 RHGSLSIGNSKNVKNNRLSALADYLRNSDDNSEEREVHLLLFSYDQRIIPSLQLPYVCCR 360

Query: 361 QALSVRHLCRYVAHHTGFQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRSTKDNFQV 420
            A SVRHLC+YVAH TGFQADEIDMLLI EL+PKIE  TSID LVSKSLIP  T+DNFQV
Sbjct: 361 PASSVRHLCQYVAHQTGFQADEIDMLLIQELHPKIEPSTSIDVLVSKSLIPHPTEDNFQV 420

Query: 421 LSEQQTLAEIKTHCSTRGQL 432
           L EQQTL E+KTHCSTRGQL
Sbjct: 421 LREQQTLGELKTHCSTRGQL 433

BLAST of Sed0003135 vs. ExPASy TrEMBL
Match: A0A6J1CDU7 (putative E3 ubiquitin-protein ligase RING1a isoform X3 OS=Momordica charantia OX=3673 GN=LOC111010638 PE=4 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 2.1e-152
Identity = 296/357 (82.91%), Postives = 320/357 (89.64%), Query Frame = 0

Query: 80  MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAALYPDIEKYEEDE 139
           MECLHRFCRECIDKSMR+GN ECPACRTHCASRRSLRDDPNYDSLI+ALYPDIEKYEEDE
Sbjct: 1   MECLHRFCRECIDKSMRMGNKECPACRTHCASRRSLRDDPNYDSLISALYPDIEKYEEDE 60

Query: 140 LAFREEERIRNKQIQASIAQTLRRQSEALSKKRPPSVRRLRG-NNYRSNHSRGKRSYRND 199
           LAFREEER+RNKQIQASIAQTL RQSEA+SKKRPPSVRRLRG NN+R+NHSRGKRSYRND
Sbjct: 61  LAFREEERVRNKQIQASIAQTLHRQSEAVSKKRPPSVRRLRGNNNHRNNHSRGKRSYRND 120

Query: 200 AEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFRKQGAAKFSQSH-FAACSDGAGE 259
           AEF  SDD EDPTAEDG KDSSSDEQ +R ERR+KGFR QGAAKFSQ    AACSDGAGE
Sbjct: 121 AEFPTSDDIEDPTAEDGGKDSSSDEQTERYERRTKGFRTQGAAKFSQPPCLAACSDGAGE 180

Query: 260 DNDSDRVHGETMG--SSRLVSSSKRLVWGKGGMRSHTRHGSMTVANNKNVKNNRLSALAD 319
           DNDS+ V+ E  G  SSR V SS+RLVWGKGGMRSHTRHGS+++ N+KNVKNNRLSALAD
Sbjct: 181 DNDSE-VNREAPGAASSRHVGSSERLVWGKGGMRSHTRHGSLSIGNSKNVKNNRLSALAD 240

Query: 320 YFRNSNNNA-EREVHLLLLSYDQRIIPSLQLPYLCCQQALSVRHLCRYVAHHTGFQADEI 379
           Y RNS++N+ EREVHLLL SYDQRIIPSLQLPY+CC+ A SVRHLC+YVAH TGFQADEI
Sbjct: 241 YLRNSDDNSEEREVHLLLFSYDQRIIPSLQLPYVCCRPASSVRHLCQYVAHQTGFQADEI 300

Query: 380 DMLLIHELNPKIELPTSIDDLVSKSLIPRSTKDNFQVLSEQQTLAEIKTHCSTRGQL 432
           DMLLI EL+PKIE  TSID LVSKSLIP  T+DNFQVL EQQTL E+KTHCSTRGQL
Sbjct: 301 DMLLIQELHPKIEPSTSIDVLVSKSLIPHPTEDNFQVLREQQTLGELKTHCSTRGQL 356

BLAST of Sed0003135 vs. ExPASy TrEMBL
Match: A0A6J1CDC8 (putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010638 PE=4 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 7.4e-137
Identity = 284/370 (76.76%), Postives = 314/370 (84.86%), Query Frame = 0

Query: 1   MPAQKRAPESSEDDPLKLGHGFS----KDEDADESDRSASSSSNEEKDEYVFYFATFRVL 60
           MPAQKRAPESSEDDPLK  HG S    +D D+++SDRS SSSSNEEKDE++        L
Sbjct: 1   MPAQKRAPESSEDDPLKRDHGCSSVHPEDGDSEQSDRSPSSSSNEEKDEFIVV-----KL 60

Query: 61  FFVFFSIDYYVALNRIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 120
             +   +   + L  IIRKTRTVMECLHRFCRECIDKSMR+GN ECPACRTHCASRRSLR
Sbjct: 61  SDIRKEVQCPICLG-IIRKTRTVMECLHRFCRECIDKSMRMGNKECPACRTHCASRRSLR 120

Query: 121 DDPNYDSLIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRPPSV 180
           DDPNYDSLI+ALYPDIEKYEEDELAFREEER+RNKQIQASIAQTL RQSEA+SKKRPPSV
Sbjct: 121 DDPNYDSLISALYPDIEKYEEDELAFREEERVRNKQIQASIAQTLHRQSEAVSKKRPPSV 180

Query: 181 RRLRG-NNYRSNHSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGF 240
           RRLRG NN+R+NHSRGKRSYRNDAEF  SDD EDPTAEDG KDSSSDEQ +R ERR+KGF
Sbjct: 181 RRLRGNNNHRNNHSRGKRSYRNDAEFPTSDDIEDPTAEDGGKDSSSDEQTERYERRTKGF 240

Query: 241 RKQGAAKFSQSH-FAACSDGAGEDNDSDRVHGETMG--SSRLVSSSKRLVWGKGGMRSHT 300
           R QGAAKFSQ    AACSDGAGEDNDS+ V+ E  G  SSR V SS+RLVWGKGGMRSHT
Sbjct: 241 RTQGAAKFSQPPCLAACSDGAGEDNDSE-VNREAPGAASSRHVGSSERLVWGKGGMRSHT 300

Query: 301 RHGSMTVANNKNVKNNRLSALADYFRNSNNNA-EREVHLLLLSYDQRIIPSLQLPYLCCQ 360
           RHGS+++ N+KNVKNNRLSALADY RNS++N+ EREVHLLL SYDQRIIPSLQLPY+CC+
Sbjct: 301 RHGSLSIGNSKNVKNNRLSALADYLRNSDDNSEEREVHLLLFSYDQRIIPSLQLPYVCCR 360

Query: 361 QALSVRHLCR 362
            A SVRHLC+
Sbjct: 361 PASSVRHLCQ 363

BLAST of Sed0003135 vs. TAIR 10
Match: AT5G44280.1 (RING 1A )

HSP 1 Score: 241.1 bits (614), Expect = 1.8e-63
Identity = 175/417 (41.97%), Postives = 253/417 (60.67%), Query Frame = 0

Query: 24  KDEDADESDRSASSSSNEEKDEYVFYFATFRVLFFVFFSIDYYVALNRIIRKTRTVMECL 83
           ++E+ D  +RS SS S ++ +         R        +   + L  II+KTRTVMECL
Sbjct: 101 EEEEEDSKERSPSSISGDQSEFMEIDLGEIRK------DVQCPICLG-IIKKTRTVMECL 160

Query: 84  HRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAALYPDIEKYEEDELAFR 143
           HRFCRECIDKSMRLGNNECPACR HCASRRSLRDDP +D+LIAAL+ +I+ YEE+ELAF 
Sbjct: 161 HRFCRECIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFH 220

Query: 144 EEERIRNKQIQASIAQTLRRQSEALSKKRPPSVR--------RLRGNNYRSNHSRGKRSY 203
           E+E  RNKQIQASIAQ  +RQSEAL K+R             R+   + R  +SR     
Sbjct: 221 EDEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQ- 280

Query: 204 RNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFRKQGAAKFSQ---SHFAACS 263
           +N +E    DDN+D       KDSSSDE+   +E R K  RK+  ++ +Q   S  A  +
Sbjct: 281 QNASEAHEDDDNDDNNNRGRDKDSSSDER--GTEVRQKKRRKRSTSRSTQHPSSSGANKN 340

Query: 264 DGAGEDNDSDRVHGETMG-SSRLVSSSKRLVWGKGGMRSHTRHGSMTV-ANNKNVKNNRL 323
           +G   DND++ V+ +T G S  LV + + L WG+GG RS+TRHG+ T   ++K+V+N R+
Sbjct: 341 NGNCADNDTE-VYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARV 400

Query: 324 SALADYFRNS--NNNAEREVHLLLLSYDQRIIPSLQLPYLCCQQALSVRHLCRYVAHHTG 383
           + L +Y R+S   ++ E ++H+ L+S D + IP L  PYLCC+  L V+ L  +VA    
Sbjct: 401 NKLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIH 460

Query: 384 FQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRS---TKDNFQVLSEQQTLAEIK 423
            + +E+++L+      ++    +I++L   ++ P S   +KD    L + +TL+ +K
Sbjct: 461 LKTEEVELLVTRR---RVGEDAAIENL--PAVTPASAAASKDEMLSLEDNETLSRLK 501

BLAST of Sed0003135 vs. TAIR 10
Match: AT1G03770.1 (RING 1B )

HSP 1 Score: 221.5 bits (563), Expect = 1.5e-57
Identity = 170/426 (39.91%), Postives = 242/426 (56.81%), Query Frame = 0

Query: 12  EDDPLKLGHGFSKDEDA-----DESDRSASSSSNE-EKDEYVFYFATFRVLFFVFFSIDY 71
           E+D +K     ++DE+A     D  +RS SSS+ E  + EY+        L  +   +  
Sbjct: 49  EEDDVKRDQVEAEDEEALGEEEDSKERSQSSSAGELSESEYMVV-----DLADICKDVQC 108

Query: 72  YVALNRIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLI 131
            + L  IIRKTRTVMECLHRFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+D+LI
Sbjct: 109 SICLG-IIRKTRTVMECLHRFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALI 168

Query: 132 AALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRP--PSVRRLRGNN 191
           AAL+ +I+K+EE+EL FR+++  RNKQIQASIAQ  +RQS+AL K++        L  + 
Sbjct: 169 AALFKNIDKFEEEELNFRQDDEARNKQIQASIAQVSQRQSKALVKRKSVGKGTAILSRSR 228

Query: 192 YRSNHSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFRKQGAAKF 251
                SR +R+ RN  E   S+ N+D       KDSSSDE  +R        +K+ A + 
Sbjct: 229 RSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKDSSSDEPCERQR------KKRSATQP 288

Query: 252 SQSHFAACSDGAGEDNDSDRVHGETMGSSRLVSSSKRLVWGKGGMRSHTRHGSMTVANNK 311
           S S+     + AG   +        + S  LV +S+ + WG+GG RS+TR G+    NN+
Sbjct: 289 SSSNANNNDNCAGNGTEQTHQRDSRVISPVLVWNSELIAWGRGGTRSNTRQGN----NNQ 348

Query: 312 NV---KNNRLSALADYFRN-SNNNAEREVHLLLLSYDQRIIPSLQLPYLCCQQALSVRHL 371
                +N RL  L +Y  +   N+ E ++HL L+S D   + +L  PYLC +  L V+ L
Sbjct: 349 GAISKRNARLKRLVEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQL 408

Query: 372 CRYVAHHTGFQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRSTKDNFQVLSEQQTLA 426
             YVA H   +A+E+++L+  + +  I   TS + +              Q L + +T+A
Sbjct: 409 REYVARHLKLKAEEVELLVSKDGDTVIGNKTSTEKM--------------QSLQDDETVA 443

BLAST of Sed0003135 vs. TAIR 10
Match: AT1G03770.3 (RING 1B )

HSP 1 Score: 214.9 bits (546), Expect = 1.4e-55
Identity = 170/432 (39.35%), Postives = 242/432 (56.02%), Query Frame = 0

Query: 12  EDDPLKLGHGFSKDEDA-----DESDRSASSSSNE-EKDEYVFYFATFRVLFFVFFSIDY 71
           E+D +K     ++DE+A     D  +RS SSS+ E  + EY+        L  +   +  
Sbjct: 49  EEDDVKRDQVEAEDEEALGEEEDSKERSQSSSAGELSESEYMVV-----DLADICKDVQC 108

Query: 72  YVALNRIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLI 131
            + L  IIRKTRTVMECLHRFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+D+LI
Sbjct: 109 SICLG-IIRKTRTVMECLHRFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALI 168

Query: 132 AALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRP--PSVRRLRGNN 191
           AAL+ +I+K+EE+EL FR+++  RNKQIQASIAQ  +RQS+AL K++        L  + 
Sbjct: 169 AALFKNIDKFEEEELNFRQDDEARNKQIQASIAQVSQRQSKALVKRKSVGKGTAILSRSR 228

Query: 192 YRSNHSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFRKQGAAKF 251
                SR +R+ RN  E   S+ N+D       KDSSSDE  +R        +K+ A + 
Sbjct: 229 RSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKDSSSDEPCERQR------KKRSATQP 288

Query: 252 SQSHFAACSDGAGEDNDSDRVHGETMGSSRLVSSSKRLVWGKGGMRSHTRHGSMTVANNK 311
           S S+     + AG   +        + S  LV +S+ + WG+GG RS+TR G+    NN+
Sbjct: 289 SSSNANNNDNCAGNGTEQTHQRDSRVISPVLVWNSELIAWGRGGTRSNTRQGN----NNQ 348

Query: 312 NV---KNNRLSALADYFRN-SNNNAEREVHLLLLSYDQRIIPSLQLPYLCCQQALSVRHL 371
                +N RL  L +Y  +   N+ E ++HL L+S D   + +L  PYLC +  L V+ L
Sbjct: 349 GAISKRNARLKRLVEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQL 408

Query: 372 CR------YVAHHTGFQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRSTKDNFQVLS 426
                   YVA H   +A+E+++L+  + +  I   TS + +              Q L 
Sbjct: 409 REVSSLPLYVARHLKLKAEEVELLVSKDGDTVIGNKTSTEKM--------------QSLQ 449

BLAST of Sed0003135 vs. TAIR 10
Match: AT1G03770.2 (RING 1B )

HSP 1 Score: 214.2 bits (544), Expect = 2.4e-55
Identity = 170/434 (39.17%), Postives = 242/434 (55.76%), Query Frame = 0

Query: 12  EDDPLKLGHGFSKDEDA-------DESDRSASSSSNE-EKDEYVFYFATFRVLFFVFFSI 71
           E+D +K     ++DE+A       D  +RS SSS+ E  + EY+        L  +   +
Sbjct: 49  EEDDVKRDQVEAEDEEALGEEEVSDSKERSQSSSAGELSESEYMVV-----DLADICKDV 108

Query: 72  DYYVALNRIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDS 131
              + L  IIRKTRTVMECLHRFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+D+
Sbjct: 109 QCSICLG-IIRKTRTVMECLHRFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDA 168

Query: 132 LIAALYPDIEKYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRP--PSVRRLRG 191
           LIAAL+ +I+K+EE+EL FR+++  RNKQIQASIAQ  +RQS+AL K++        L  
Sbjct: 169 LIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQVSQRQSKALVKRKSVGKGTAILSR 228

Query: 192 NNYRSNHSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFRKQGAA 251
           +      SR +R+ RN  E   S+ N+D       KDSSSDE  +R        +K+ A 
Sbjct: 229 SRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKDSSSDEPCERQR------KKRSAT 288

Query: 252 KFSQSHFAACSDGAGEDNDSDRVHGETMGSSRLVSSSKRLVWGKGGMRSHTRHGSMTVAN 311
           + S S+     + AG   +        + S  LV +S+ + WG+GG RS+TR G+    N
Sbjct: 289 QPSSSNANNNDNCAGNGTEQTHQRDSRVISPVLVWNSELIAWGRGGTRSNTRQGN----N 348

Query: 312 NKNV---KNNRLSALADYFRN-SNNNAEREVHLLLLSYDQRIIPSLQLPYLCCQQALSVR 371
           N+     +N RL  L +Y  +   N+ E ++HL L+S D   + +L  PYLC +  L V+
Sbjct: 349 NQGAISKRNARLKRLVEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVK 408

Query: 372 HLCR------YVAHHTGFQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRSTKDNFQV 426
            L        YVA H   +A+E+++L+  + +  I   TS + +              Q 
Sbjct: 409 QLREVSSLPLYVARHLKLKAEEVELLVSKDGDTVIGNKTSTEKM--------------QS 451

BLAST of Sed0003135 vs. TAIR 10
Match: AT5G44280.2 (RING 1A )

HSP 1 Score: 180.6 bits (457), Expect = 3.0e-45
Identity = 154/427 (36.07%), Postives = 235/427 (55.04%), Query Frame = 0

Query: 24  KDEDADESDRSASSSSNEEKDEYVFYFATFRV----------LFFVFFSIDYYVALNRII 83
           ++E+ D  +RS SS S ++ +         R           + F+   I YY       
Sbjct: 101 EEEEEDSKERSPSSISGDQSEFMEIDLGEIRKDVQCPICLVGMKFLRLFISYY------- 160

Query: 84  RKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDSLIAALYPDIE 143
           ++ +       +FC           NNECPACR HCASRRSLRDDP +D+LIAAL+ +I+
Sbjct: 161 KENKDCDGMSPQFC-------ATFKNNECPACRKHCASRRSLRDDPKFDALIAALFTNID 220

Query: 144 KYEEDELAFREEERIRNKQIQASIAQTLRRQSEALSKKRPPSVR--------RLRGNNYR 203
            YEE+ELAF E+E  RNKQIQASIAQ  +RQSEAL K+R             R+   + R
Sbjct: 221 SYEEEELAFHEDEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRR 280

Query: 204 SNHSRGKRSYRNDAEFLASDDNEDPTAEDGVKDSSSDEQMDRSERRSKGFRKQGAAKFSQ 263
             +SR     +N +E    DDN+D       KDSSSDE+   +E R K  RK+  ++ +Q
Sbjct: 281 RRNSRNMEQ-QNASEAHEDDDNDDNNNRGRDKDSSSDER--GTEVRQKKRRKRSTSRSTQ 340

Query: 264 ---SHFAACSDGAGEDNDSDRVHGETMG-SSRLVSSSKRLVWGKGGMRSHTRHGSMTV-A 323
              S  A  ++G   DND++ V+ +T G S  LV + + L WG+GG RS+TRHG+ T   
Sbjct: 341 HPSSSGANKNNGNCADNDTE-VYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGG 400

Query: 324 NNKNVKNNRLSALADYFRNS--NNNAEREVHLLLLSYDQRIIPSLQLPYLCCQQALSVRH 383
           ++K+V+N R++ L +Y R+S   ++ E ++H+ L+S D + IP L  PYLCC+  L V+ 
Sbjct: 401 SSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQ 460

Query: 384 LCRYVAHHTGFQADEIDMLLIHELNPKIELPTSIDDLVSKSLIPRS---TKDNFQVLSEQ 423
           L  +VA     + +E+++L+      ++    +I++L   ++ P S   +KD    L + 
Sbjct: 461 LREFVALQIHLKTEEVELLVTRR---RVGEDAAIENL--PAVTPASAAASKDEMLSLEDN 504