Sed0002781 (gene) Chayote v1

BLAST of Sed0002781 vs. NCBI nr
Match: XP_038905830.1 (uncharacterized protein LOC120091781 [Benincasa hispida])

HSP 1 Score: 847.4 bits (2188), Expect = 6.6e-242
Identity = 424/522 (81.23%), Postives = 470/522 (90.04%), Query Frame = 0

Query: 14  NPTMSLPFYDDNLYILNMKNDIEAKNEEVVPSLEHLLATDEATIPPISAVKQLAVAVRAA 73
           NPTM++PFYD+NLY+LNMKNDIEAKN E+VPS+E  LA D+A+IPP+S +KQLA+AV A 
Sbjct: 102 NPTMAVPFYDENLYLLNMKNDIEAKNAEIVPSVESFLAIDKASIPPLSVIKQLAIAVEAG 161

Query: 74  KKSKSLKTLLASASSSPHSSPAKDKSGLSLSAVRAFMLPEKEAKVSTAFGDDDRVQALIR 133
           KKSKS+K+L+A   SS  SSP ++KSGLSLS+VRA ML EKE K STAF  D+R+Q+LI 
Sbjct: 162 KKSKSMKSLVA---SSGDSSP-REKSGLSLSSVRALMLREKEEKSSTAFRHDERIQSLIC 221

Query: 134 SLFHADGVFLRRTLTSASDHTILTSLPRDIHGAPPHSFLVKISEVIGSFRTLRKMTLFWC 193
           SLF A+G FL+R+  +AS+ TI+TSLP+DIHGAPP S LVKISEVIGSFR++RKM LFWC
Sbjct: 222 SLFDAEGDFLKRSFGTASEGTIVTSLPKDIHGAPPDSLLVKISEVIGSFRSVRKMALFWC 281

Query: 194 RIVDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIATDLLDAVL 253
           RIVDE+RRFW EE+Y+P IPIDEIPDLNSCLLYQRFQVINCC+SRKRRHEIATD LDA L
Sbjct: 282 RIVDEMRRFWSEEQYLPDIPIDEIPDLNSCLLYQRFQVINCCVSRKRRHEIATDSLDAAL 341

Query: 254 RVASSNTELRISEVTVPTNSLLYARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQE 313
           R ASSN E R SE+T+P N+LLYARLNNGELALR+GADC F N KMLETGE VYSPVTQE
Sbjct: 342 REASSNAESRTSEITIPGNTLLYARLNNGELALRLGADCPFGNHKMLETGEAVYSPVTQE 401

Query: 314 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 373
           GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP
Sbjct: 402 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 461

Query: 374 EPSNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILN 433
           EPSN SIDS +G+DSRGQLSSRMQKEGNLWRELWETSKPVPAVKQ PLFDEDL VEGILN
Sbjct: 462 EPSNDSIDSPVGSDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQAPLFDEDLVVEGILN 521

Query: 434 DLEDLPPSELYEPLFLSLLGLGFIMAEAKLAKDNDLSKMFYECKDYVVATCQGSFWSNKV 493
           DLEDLPPSEL+EPLF+SLLGLGFIMAE KL K+N+LSK+FY+CK YVVATCQGS WSNKV
Sbjct: 522 DLEDLPPSELFEPLFISLLGLGFIMAEEKLGKNNNLSKLFYDCKGYVVATCQGSSWSNKV 581

Query: 494 DDICQVYETVETMMVNPAEILNAMKQPEESNMTASELKRRLR 536
           DDICQVYETVETMMVNP EIL AMKQPEESNMTASELKRR +
Sbjct: 582 DDICQVYETVETMMVNPEEILKAMKQPEESNMTASELKRRFK 619

BLAST of Sed0002781 vs. NCBI nr
Match: XP_004149589.1 (rab3 GTPase-activating protein catalytic subunit isoform X3 [Cucumis sativus])

HSP 1 Score: 844.0 bits (2179), Expect = 7.3e-241
Identity = 420/522 (80.46%), Postives = 467/522 (89.46%), Query Frame = 0

Query: 14  NPTMSLPFYDDNLYILNMKNDIEAKNEEVVPSLEHLLATDEATIPPISAVKQLAVAVRAA 73
           NPTM++PFYD+NLY+LNMKNDIEAKN E++PS+E L  TD+ +IPP+S +KQLA AV A 
Sbjct: 102 NPTMAVPFYDENLYLLNMKNDIEAKNAEIIPSVESLWTTDKDSIPPLSVIKQLATAVEAG 161

Query: 74  KKSKSLKTLLASASSSPHSSPAKDKSGLSLSAVRAFMLPEKEAKVSTAFGDDDRVQALIR 133
           KKSKS+K+LLA   SS  SSPA++KSGLSLS+VRA ML E+E K ST F  D+R+Q+LI 
Sbjct: 162 KKSKSMKSLLA---SSGDSSPAREKSGLSLSSVRALMLREREEKSSTEFRHDERIQSLIC 221

Query: 134 SLFHADGVFLRRTLTSASDHTILTSLPRDIHGAPPHSFLVKISEVIGSFRTLRKMTLFWC 193
           SLF A+GVFLRR   +AS+ T +TSLP+DIHGAPP S LVKISEVIGSFRTLRKM LFWC
Sbjct: 222 SLFDAEGVFLRRYFDTASEGTFVTSLPKDIHGAPPDSLLVKISEVIGSFRTLRKMALFWC 281

Query: 194 RIVDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIATDLLDAVL 253
           RIVDE+RRFW EE+Y+PGIPIDEIPDLNSCLLYQR QVINCC+SRKRRHEIATD +DA L
Sbjct: 282 RIVDEMRRFWSEEQYLPGIPIDEIPDLNSCLLYQRLQVINCCVSRKRRHEIATDSIDAAL 341

Query: 254 RVASSNTELRISEVTVPTNSLLYARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQE 313
           R ASSN E + SEVT+P N+LLYARLNNGELALR+GADC F N KMLETGE VYSPVTQE
Sbjct: 342 REASSNAESKTSEVTIPGNTLLYARLNNGELALRLGADCPFGNHKMLETGEAVYSPVTQE 401

Query: 314 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 373
           GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP
Sbjct: 402 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 461

Query: 374 EPSNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILN 433
           EPSN SIDS +G+DSRGQLSSRMQKEGNLW ELWETSKPVPAVKQTPLFDEDL VEGILN
Sbjct: 462 EPSNDSIDSPVGSDSRGQLSSRMQKEGNLWLELWETSKPVPAVKQTPLFDEDLVVEGILN 521

Query: 434 DLEDLPPSELYEPLFLSLLGLGFIMAEAKLAKDNDLSKMFYECKDYVVATCQGSFWSNKV 493
           DLEDLPPSEL++PLF+SLLGLGFI+AEAKLA +N+LSK+FY+CK YVVATCQ S WSNKV
Sbjct: 522 DLEDLPPSELFQPLFISLLGLGFIVAEAKLANNNNLSKLFYDCKGYVVATCQNSSWSNKV 581

Query: 494 DDICQVYETVETMMVNPAEILNAMKQPEESNMTASELKRRLR 536
           DD+CQVYETVETMMVNP EIL A+KQPEESNMTASELKRR +
Sbjct: 582 DDLCQVYETVETMMVNPEEILKAIKQPEESNMTASELKRRFK 620

BLAST of Sed0002781 vs. NCBI nr
Match: KAE8650118.1 (hypothetical protein Csa_011035 [Cucumis sativus])

HSP 1 Score: 844.0 bits (2179), Expect = 7.3e-241
Identity = 420/522 (80.46%), Postives = 467/522 (89.46%), Query Frame = 0

Query: 14  NPTMSLPFYDDNLYILNMKNDIEAKNEEVVPSLEHLLATDEATIPPISAVKQLAVAVRAA 73
           NPTM++PFYD+NLY+LNMKNDIEAKN E++PS+E L  TD+ +IPP+S +KQLA AV A 
Sbjct: 97  NPTMAVPFYDENLYLLNMKNDIEAKNAEIIPSVESLWTTDKDSIPPLSVIKQLATAVEAG 156

Query: 74  KKSKSLKTLLASASSSPHSSPAKDKSGLSLSAVRAFMLPEKEAKVSTAFGDDDRVQALIR 133
           KKSKS+K+LLA   SS  SSPA++KSGLSLS+VRA ML E+E K ST F  D+R+Q+LI 
Sbjct: 157 KKSKSMKSLLA---SSGDSSPAREKSGLSLSSVRALMLREREEKSSTEFRHDERIQSLIC 216

Query: 134 SLFHADGVFLRRTLTSASDHTILTSLPRDIHGAPPHSFLVKISEVIGSFRTLRKMTLFWC 193
           SLF A+GVFLRR   +AS+ T +TSLP+DIHGAPP S LVKISEVIGSFRTLRKM LFWC
Sbjct: 217 SLFDAEGVFLRRYFDTASEGTFVTSLPKDIHGAPPDSLLVKISEVIGSFRTLRKMALFWC 276

Query: 194 RIVDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIATDLLDAVL 253
           RIVDE+RRFW EE+Y+PGIPIDEIPDLNSCLLYQR QVINCC+SRKRRHEIATD +DA L
Sbjct: 277 RIVDEMRRFWSEEQYLPGIPIDEIPDLNSCLLYQRLQVINCCVSRKRRHEIATDSIDAAL 336

Query: 254 RVASSNTELRISEVTVPTNSLLYARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQE 313
           R ASSN E + SEVT+P N+LLYARLNNGELALR+GADC F N KMLETGE VYSPVTQE
Sbjct: 337 REASSNAESKTSEVTIPGNTLLYARLNNGELALRLGADCPFGNHKMLETGEAVYSPVTQE 396

Query: 314 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 373
           GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP
Sbjct: 397 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 456

Query: 374 EPSNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILN 433
           EPSN SIDS +G+DSRGQLSSRMQKEGNLW ELWETSKPVPAVKQTPLFDEDL VEGILN
Sbjct: 457 EPSNDSIDSPVGSDSRGQLSSRMQKEGNLWLELWETSKPVPAVKQTPLFDEDLVVEGILN 516

Query: 434 DLEDLPPSELYEPLFLSLLGLGFIMAEAKLAKDNDLSKMFYECKDYVVATCQGSFWSNKV 493
           DLEDLPPSEL++PLF+SLLGLGFI+AEAKLA +N+LSK+FY+CK YVVATCQ S WSNKV
Sbjct: 517 DLEDLPPSELFQPLFISLLGLGFIVAEAKLANNNNLSKLFYDCKGYVVATCQNSSWSNKV 576

Query: 494 DDICQVYETVETMMVNPAEILNAMKQPEESNMTASELKRRLR 536
           DD+CQVYETVETMMVNP EIL A+KQPEESNMTASELKRR +
Sbjct: 577 DDLCQVYETVETMMVNPEEILKAIKQPEESNMTASELKRRFK 615

BLAST of Sed0002781 vs. NCBI nr
Match: XP_023521287.1 (rab3 GTPase-activating protein catalytic subunit-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023521288.1 rab3 GTPase-activating protein catalytic subunit-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 842.0 bits (2174), Expect = 2.8e-240
Identity = 423/519 (81.50%), Postives = 465/519 (89.60%), Query Frame = 0

Query: 17  MSLPFYDDNLYILNMKNDIEAKNEEVVPSLEHLLATDEATIPPISAVKQLAVAVRAAKKS 76
           MS+PFYD+NLY+LNMKNDIEAKN E++PS+E  LATD+A+IP +S +KQLA+A+ A KKS
Sbjct: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60

Query: 77  KSLKTLLASASSSPHSSPAKDKSGLSLSAVRAFMLPEKEAKVSTAFGDDDRVQALIRSLF 136
           KS+K+LLAS++    SS AK K GL+LS VRA +L EKE K+ST F  D+R+Q+LIRSLF
Sbjct: 61  KSVKSLLASSAG---SSSAKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLF 120

Query: 137 HADGVFLRRTLTSASDHTILTSLPRDIHGAPPHSFLVKISEVIGSFRTLRKMTLFWCRIV 196
            A G FL+R++TS S+ TI  SLPRDIHGAPP S LVKISEVIGSFR+LRKMTLFWCRIV
Sbjct: 121 DAGGDFLKRSITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIV 180

Query: 197 DEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIATDLLDAVLRVA 256
           DE+RRFW EE+YIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIA+D LD VLRVA
Sbjct: 181 DEMRRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVA 240

Query: 257 SSNTELRISEVTVPTNSLLYARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQEGPL 316
           SSNTE   SEVTVP NSLLYARLNNGELALR+GADC F+NL MLETGE VYSPVTQEGPL
Sbjct: 241 SSNTESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPVTQEGPL 300

Query: 317 LTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPS 376
           LTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPS
Sbjct: 301 LTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPS 360

Query: 377 NGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILNDLE 436
           NGS+ SSI +DSRGQLSSRMQKEGNLWRELW+TSK VPAVKQTPLFDEDL VEGIL DLE
Sbjct: 361 NGSVHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLE 420

Query: 437 DLPPSELYEPLFLSLLGLGFIMAEAKLAKDNDLSKMFYECKDYVVATCQGSFWSNKVDDI 496
           DLP +ELY PLFLSLLGLGF+MAEAKLA+DN+LSK+FYECKDYVVATCQGS W+NKVDDI
Sbjct: 421 DLPTTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDI 480

Query: 497 CQVYETVETMMVNPAEILNAMKQPEESNMTASELKRRLR 536
           CQVYETVETMMVNP EIL AMKQ EESNMT S LKRR +
Sbjct: 481 CQVYETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFK 516

BLAST of Sed0002781 vs. NCBI nr
Match: XP_008449120.1 (PREDICTED: rab3 GTPase-activating protein catalytic subunit isoform X2 [Cucumis melo] >TYJ96562.1 rab3 GTPase-activating protein catalytic subunit isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 841.6 bits (2173), Expect = 3.6e-240
Identity = 420/522 (80.46%), Postives = 465/522 (89.08%), Query Frame = 0

Query: 14  NPTMSLPFYDDNLYILNMKNDIEAKNEEVVPSLEHLLATDEATIPPISAVKQLAVAVRAA 73
           NPTM++PFYD+NLY+LNMKNDIEAKN E++PS+E L  TD  +IPP+S +KQLA AV AA
Sbjct: 102 NPTMAVPFYDENLYLLNMKNDIEAKNAEIIPSVESLWTTDTDSIPPLSVIKQLATAVEAA 161

Query: 74  KKSKSLKTLLASASSSPHSSPAKDKSGLSLSAVRAFMLPEKEAKVSTAFGDDDRVQALIR 133
           KKSKS+K+LLA   SS  SSPA++KSGLSLS+VRA ML EKE K ST F  D+R+Q+LI 
Sbjct: 162 KKSKSMKSLLA---SSGDSSPAREKSGLSLSSVRALMLREKEEKSSTEFHHDERIQSLIC 221

Query: 134 SLFHADGVFLRRTLTSASDHTILTSLPRDIHGAPPHSFLVKISEVIGSFRTLRKMTLFWC 193
           SLF A+G+FLRR   +A + TI+TSLP+DIHGAPP S LVKISEVIGSFRTLRKM LFWC
Sbjct: 222 SLFDAEGIFLRRNFGTALEDTIVTSLPKDIHGAPPDSLLVKISEVIGSFRTLRKMALFWC 281

Query: 194 RIVDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIATDLLDAVL 253
           RIVDE+RRFW EE+Y+PGIPIDEIPDLNSCLLYQR QVINCC+SRKRRHEIATD +DA +
Sbjct: 282 RIVDEMRRFWSEEQYLPGIPIDEIPDLNSCLLYQRLQVINCCVSRKRRHEIATDSIDAAI 341

Query: 254 RVASSNTELRISEVTVPTNSLLYARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQE 313
           R ASSN E   S+VT+P N+LLYARLNNGELALR+GADC F + KMLETGE VYSPVTQE
Sbjct: 342 REASSNAESGTSKVTIPGNTLLYARLNNGELALRLGADCPFGDHKMLETGEAVYSPVTQE 401

Query: 314 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 373
           GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP
Sbjct: 402 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 461

Query: 374 EPSNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILN 433
           EPSN SIDS +G+DSRGQLSSRMQKEGNLW ELWETSKPVPAVKQTPLFDEDL VEGILN
Sbjct: 462 EPSNDSIDSPVGSDSRGQLSSRMQKEGNLWLELWETSKPVPAVKQTPLFDEDLVVEGILN 521

Query: 434 DLEDLPPSELYEPLFLSLLGLGFIMAEAKLAKDNDLSKMFYECKDYVVATCQGSFWSNKV 493
           DLEDLPPSEL+EPLF+SLLGLG IMAEAKLA +N+LSK+FY+CK YVVATCQ S WSNKV
Sbjct: 522 DLEDLPPSELFEPLFISLLGLGLIMAEAKLANNNNLSKLFYDCKGYVVATCQSSSWSNKV 581

Query: 494 DDICQVYETVETMMVNPAEILNAMKQPEESNMTASELKRRLR 536
           DD+CQVYETVETMMVNP EIL AMKQPEESNMTASELKRR +
Sbjct: 582 DDLCQVYETVETMMVNPEEILKAMKQPEESNMTASELKRRFK 620

BLAST of Sed0002781 vs. ExPASy Swiss-Prot
Match: Q80UJ7 (Rab3 GTPase-activating protein catalytic subunit OS=Mus musculus OX=10090 GN=Rab3gap1 PE=1 SV=4)

HSP 1 Score: 151.8 bits (382), Expect = 2.3e-35
Identity = 106/337 (31.45%), Postives = 158/337 (46.88%), Query Frame = 0

Query: 185 LRKMTLFWCRIVDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEI 244
           L+ +   W   V E+R  W     IPG+     PDL  CLL+Q+ Q++NCC+ RK+  + 
Sbjct: 473 LKGVAHLWQEFVLEMRFRWENNFLIPGLASGS-PDLRCCLLHQKLQMLNCCIERKKARDE 532

Query: 245 A--TDLLDAVLRV--------------------------------ASSNTELRISEVTVP 304
              T L D+                                    + S++E    E    
Sbjct: 533 GKKTSLSDSTTSAYPGDAGKTGGQLGLDHLRDTEKEKGEVGKSWDSWSDSEEEFFECLSD 592

Query: 305 TNSLLYARLNNGELALRIGADCEFTNLK------------MLETGETVYSPVTQEGPLLT 364
           T  L      NG+ + + G   E  NLK            +L  GE +Y PVTQE   +T
Sbjct: 593 TEDL----KGNGQESGKKGGPKEMANLKPEGRLHQHGKLTLLHNGEPLYIPVTQEPAPMT 652

Query: 365 EDVIKETEEFVLRTGSVGAGC--------SQLLSDMQAFKAANPGCILEDFVRWHSPPDW 424
           ED+++E  E + + G+   G         + LLSDM++FKAANPGC LEDFVRW+SP D+
Sbjct: 653 EDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCFLEDFVRWYSPRDY 712

Query: 425 TEPEPSNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEG 468
            E E +    D       +G+LS+RM+   N+W E WET+KPVPA +Q  LFD+    E 
Sbjct: 713 IEEEVT----DEKGNVVLKGELSARMKIPSNMWVEAWETAKPVPARRQRRLFDDTREAEK 772

BLAST of Sed0002781 vs. ExPASy Swiss-Prot
Match: Q15042 (Rab3 GTPase-activating protein catalytic subunit OS=Homo sapiens OX=9606 GN=RAB3GAP1 PE=1 SV=3)

HSP 1 Score: 149.8 bits (377), Expect = 8.6e-35
Identity = 105/336 (31.25%), Postives = 159/336 (47.32%), Query Frame = 0

Query: 185 LRKMTLFWCRIVDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHE- 244
           L+ +   W   V E+R  W     IPG+     PDL  CLL+Q+ Q++NCC+ RK+  + 
Sbjct: 474 LKGVAHLWQEFVLEMRFRWENNFLIPGLASGP-PDLRCCLLHQKLQMLNCCIERKKARDE 533

Query: 245 ----IATDLLDAVLRVAS----------------------------SNTELRISEVTVPT 304
                A+D+ +     A                             S++E    E    T
Sbjct: 534 GKKTSASDVTNIYPGDAGKAGDQLVPDNLKETDKEKGEVGKSWDSWSDSEEEFFECLSDT 593

Query: 305 NSLLYARLNNGELALRIGADCEFTNLK------------MLETGETVYSPVTQEGPLLTE 364
             L      NG+ + + G   E  NL+            +L  GE +Y PVTQE   +TE
Sbjct: 594 EEL----KGNGQESGKKGGPKEMANLRPEGRLYQHGKLTLLHNGEPLYIPVTQEPAPMTE 653

Query: 365 DVIKETEEFVLRTGSVGAGC--------SQLLSDMQAFKAANPGCILEDFVRWHSPPDWT 424
           D+++E  E + + G+   G         + LLSDM++FKAANPGC LEDFVRW+SP D+ 
Sbjct: 654 DLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCSLEDFVRWYSPRDYI 713

Query: 425 EPEPSNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGI 468
           E E     ID       +G+LS+RM+   N+W E WET+KP+PA +Q  LFD+    E +
Sbjct: 714 EEE----VIDEKGNVVLKGELSARMKIPSNMWVEAWETAKPIPARRQRRLFDDTREAEKV 773

BLAST of Sed0002781 vs. ExPASy Swiss-Prot
Match: Q642R9 (Rab3 GTPase-activating protein catalytic subunit OS=Xenopus laevis OX=8355 GN=rab3gap1 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 1.1e-34
Identity = 131/446 (29.37%), Postives = 206/446 (46.19%), Query Frame = 0

Query: 78  SLKTLLASASSSPHSSPAKDKSGLS---LSAVRAFMLPE------KEAKVSTAFGDDDRV 137
           S+ +++ SA          D+S L+   L+A+  F+ P+       EAK ST+ G+    
Sbjct: 378 SVTSMVHSARKKIRKHRGADESPLNNDVLNAILFFLFPDTKSLDGSEAKPSTSTGN---- 437

Query: 138 QALIRSLFHADGVFLRRTLTSASDHTILTSLPRDIHGAPPHSFLVKISEVIGSFRTLRKM 197
              I S   ++   L   L SA  +++   L            L  ++   G  + +  +
Sbjct: 438 ---ISSQSESEDYNLYSQLKSAPSNSLTYKLA---------LCLCMVNFYHGGVKGVAHL 497

Query: 198 TLFWCRIVDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRR------- 257
              W   V E+R  W     IPG+  +  PDL  CLL+Q+ Q++NCCL RK+        
Sbjct: 498 ---WQEFVLEMRYRWENNFLIPGL-ANGSPDLKCCLLHQKLQMLNCCLERKKARDEGKKG 557

Query: 258 -----------HEIATDLLDAV------LRVAS-----SNTELRISEVTVPTNSLLYARL 317
                      ++ A+DLL  V        VA      S++E    E    T  L  +  
Sbjct: 558 NPLYSSSESSVNKTASDLLSPVEADKSKYEVAKSWDSWSDSEEEFFECHSDTEELKESGQ 617

Query: 318 NNGELALR----------IGADCEFTNLKMLETGETVYSPVTQEGPLLTEDVIKETEEFV 377
            +   A             G   +  NL +L +GE +Y PVTQ+   +T+D+++E  E +
Sbjct: 618 ESARKAKEETKENPSPKPEGRLHQSGNLMLLNSGEPLYIPVTQDPAPMTDDLLEEQSEVL 677

Query: 378 LRTGSVGAGC--------SQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGSID 437
            + G+   G         + LLSDM++FKAANPGC LEDFVRW+SP D+ E E     +D
Sbjct: 678 AKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCCLEDFVRWYSPRDYIEEE----VMD 737

Query: 438 SSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILNDLEDLPPS 467
                  +G+LS+RM+   N+W E WET+KP+PA +Q  LFD+    E +L+ L    P+
Sbjct: 738 DKGNKIFKGELSARMKIPNNMWVEAWETAKPIPARRQRRLFDDTKEAEKVLHYLAVQKPA 797

BLAST of Sed0002781 vs. ExPASy Swiss-Prot
Match: P69735 (Rab3 GTPase-activating protein catalytic subunit (Fragments) OS=Rattus norvegicus OX=10116 GN=Rab3gap1 PE=1 SV=2)

HSP 1 Score: 145.2 bits (365), Expect = 2.1e-33
Identity = 102/333 (30.63%), Postives = 158/333 (47.45%), Query Frame = 0

Query: 185 LRKMTLFWCRIVDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEI 244
           L+ +   W   V E+R  W     IPG+     PDL  CLL+Q+ Q++NCC+ RK+  + 
Sbjct: 267 LKGVAHLWQEFVLEMRFRWENNFLIPGLASGP-PDLRCCLLHQKLQMLNCCIERKKARDE 326

Query: 245 A--TDLLDAVLRVASS---------------NTELRISEVTVPTNSLLYAR--------- 304
              T   D++ +   +               +TE    E     +S   +          
Sbjct: 327 GKKTSPSDSMTKAYPADAGKAGGQLGLDHLRDTEKEKGEAGKSWDSWSDSEEEFFECLSD 386

Query: 305 ----LNNGELALRIGADCEFTNLK------------MLETGETVYSPVTQEGPLLTEDVI 364
                 NG+ + + G   +   LK            +L  GE +Y PVTQE   +TED++
Sbjct: 387 AEDLRGNGQESTKKGGPKDMAPLKPEGRLHQHGKLTLLRNGEPLYIPVTQEPAPMTEDLL 446

Query: 365 KETEEFVLRTGSVGAGC--------SQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPE 424
           +E  E + + G+   G         + LLSDM++FKAANPGC LEDFVRW+SP D+ E E
Sbjct: 447 EEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCCLEDFVRWYSPRDYIEEE 506

Query: 425 PSNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILND 468
            +    D       +G+LS+RM+   N+W E WET+KPVPA +Q  LFD+    E +L+ 
Sbjct: 507 VT----DEKGNVVLKGELSARMKIPSNMWVEAWETAKPVPARRQRRLFDDTREAEKVLHY 566

BLAST of Sed0002781 vs. ExPASy Swiss-Prot
Match: Q9VQ26 (Rab3 GTPase-activating protein catalytic subunit OS=Drosophila melanogaster OX=7227 GN=CG31935 PE=1 SV=2)

HSP 1 Score: 140.2 bits (352), Expect = 6.8e-32
Identity = 107/341 (31.38%), Postives = 163/341 (47.80%), Query Frame = 0

Query: 160 PRDIHGAPPHSFLVKISEVIGS----FRTLRKMTLFWCRIVDEIRRFWFEERYIPGIPID 219
           P  I  A P S + ++S ++ +      ++  M   W     ++R  W     +PGI   
Sbjct: 442 PMRIKSAVPDSLVCRLSCLLATCHAHLGSVEGMAQVWAAFTRQLRLLWDNSLMVPGISAG 501

Query: 220 EIPDLNSCLLYQRFQVINCCLSRK-RRHEIATDLLDAVLRVASSNTE----------LRI 279
             PD  +CLL+Q+ Q++N C+ R+ +R   +    + ++  ASS  E             
Sbjct: 502 -FPDTRTCLLHQKLQMLNVCVERRVQREANSKRKSEGMVGKASSEEEEDEDDDEGEFFDC 561

Query: 280 SEVTV----PTNSLL-------YARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQE 339
            ++T     PT ++L         RLNN  L              + E  E +Y P TQE
Sbjct: 562 DDLTAGAGSPTKAVLSLKPEGRLRRLNNERL--------------LEEPDEYLYIPDTQE 621

Query: 340 GPLLTEDVIKETEEFVLRTGSVGAG------CSQLLSDMQAFKAANPGCILEDFVRWHSP 399
               TED +++  E +L+ G  G+G      C+ LLSDM+AFKAANP  I+EDF+RW+SP
Sbjct: 622 PVPKTEDQLQDDAEVMLKLGP-GSGLTTQMMCTSLLSDMEAFKAANPRGIMEDFIRWYSP 681

Query: 400 PDWTEPEPSNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLA 459
            DW E     G +        + QLS RM  EGN W+++WE ++ VP  +Q  LFD+   
Sbjct: 682 KDWEEVTDELGQV--------KHQLSIRMTTEGNTWQKVWEQAQAVPVSRQKRLFDDTNE 741

Query: 460 VEGILNDLEDLPPSELYE----PLFLS-LLGLGFIMAEAKL 464
              +L+ LE     E+Y     PL  S +L L  I++ A+L
Sbjct: 742 ALKVLHYLETRKMHEIYNLTVIPLLHSAILKLADILSNAEL 758

BLAST of Sed0002781 vs. ExPASy TrEMBL
Match: A0A0A0L2W4 (Rab3 GTPase-activating protein catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3G064220 PE=3 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 3.5e-241
Identity = 420/522 (80.46%), Postives = 467/522 (89.46%), Query Frame = 0

Query: 14  NPTMSLPFYDDNLYILNMKNDIEAKNEEVVPSLEHLLATDEATIPPISAVKQLAVAVRAA 73
           NPTM++PFYD+NLY+LNMKNDIEAKN E++PS+E L  TD+ +IPP+S +KQLA AV A 
Sbjct: 102 NPTMAVPFYDENLYLLNMKNDIEAKNAEIIPSVESLWTTDKDSIPPLSVIKQLATAVEAG 161

Query: 74  KKSKSLKTLLASASSSPHSSPAKDKSGLSLSAVRAFMLPEKEAKVSTAFGDDDRVQALIR 133
           KKSKS+K+LLA   SS  SSPA++KSGLSLS+VRA ML E+E K ST F  D+R+Q+LI 
Sbjct: 162 KKSKSMKSLLA---SSGDSSPAREKSGLSLSSVRALMLREREEKSSTEFRHDERIQSLIC 221

Query: 134 SLFHADGVFLRRTLTSASDHTILTSLPRDIHGAPPHSFLVKISEVIGSFRTLRKMTLFWC 193
           SLF A+GVFLRR   +AS+ T +TSLP+DIHGAPP S LVKISEVIGSFRTLRKM LFWC
Sbjct: 222 SLFDAEGVFLRRYFDTASEGTFVTSLPKDIHGAPPDSLLVKISEVIGSFRTLRKMALFWC 281

Query: 194 RIVDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIATDLLDAVL 253
           RIVDE+RRFW EE+Y+PGIPIDEIPDLNSCLLYQR QVINCC+SRKRRHEIATD +DA L
Sbjct: 282 RIVDEMRRFWSEEQYLPGIPIDEIPDLNSCLLYQRLQVINCCVSRKRRHEIATDSIDAAL 341

Query: 254 RVASSNTELRISEVTVPTNSLLYARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQE 313
           R ASSN E + SEVT+P N+LLYARLNNGELALR+GADC F N KMLETGE VYSPVTQE
Sbjct: 342 REASSNAESKTSEVTIPGNTLLYARLNNGELALRLGADCPFGNHKMLETGEAVYSPVTQE 401

Query: 314 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 373
           GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP
Sbjct: 402 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 461

Query: 374 EPSNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILN 433
           EPSN SIDS +G+DSRGQLSSRMQKEGNLW ELWETSKPVPAVKQTPLFDEDL VEGILN
Sbjct: 462 EPSNDSIDSPVGSDSRGQLSSRMQKEGNLWLELWETSKPVPAVKQTPLFDEDLVVEGILN 521

Query: 434 DLEDLPPSELYEPLFLSLLGLGFIMAEAKLAKDNDLSKMFYECKDYVVATCQGSFWSNKV 493
           DLEDLPPSEL++PLF+SLLGLGFI+AEAKLA +N+LSK+FY+CK YVVATCQ S WSNKV
Sbjct: 522 DLEDLPPSELFQPLFISLLGLGFIVAEAKLANNNNLSKLFYDCKGYVVATCQNSSWSNKV 581

Query: 494 DDICQVYETVETMMVNPAEILNAMKQPEESNMTASELKRRLR 536
           DD+CQVYETVETMMVNP EIL A+KQPEESNMTASELKRR +
Sbjct: 582 DDLCQVYETVETMMVNPEEILKAIKQPEESNMTASELKRRFK 620

BLAST of Sed0002781 vs. ExPASy TrEMBL
Match: A0A5D3B9M0 (Rab3 GTPase-activating protein catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G002240 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.8e-240
Identity = 420/522 (80.46%), Postives = 465/522 (89.08%), Query Frame = 0

Query: 14  NPTMSLPFYDDNLYILNMKNDIEAKNEEVVPSLEHLLATDEATIPPISAVKQLAVAVRAA 73
           NPTM++PFYD+NLY+LNMKNDIEAKN E++PS+E L  TD  +IPP+S +KQLA AV AA
Sbjct: 102 NPTMAVPFYDENLYLLNMKNDIEAKNAEIIPSVESLWTTDTDSIPPLSVIKQLATAVEAA 161

Query: 74  KKSKSLKTLLASASSSPHSSPAKDKSGLSLSAVRAFMLPEKEAKVSTAFGDDDRVQALIR 133
           KKSKS+K+LLA   SS  SSPA++KSGLSLS+VRA ML EKE K ST F  D+R+Q+LI 
Sbjct: 162 KKSKSMKSLLA---SSGDSSPAREKSGLSLSSVRALMLREKEEKSSTEFHHDERIQSLIC 221

Query: 134 SLFHADGVFLRRTLTSASDHTILTSLPRDIHGAPPHSFLVKISEVIGSFRTLRKMTLFWC 193
           SLF A+G+FLRR   +A + TI+TSLP+DIHGAPP S LVKISEVIGSFRTLRKM LFWC
Sbjct: 222 SLFDAEGIFLRRNFGTALEDTIVTSLPKDIHGAPPDSLLVKISEVIGSFRTLRKMALFWC 281

Query: 194 RIVDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIATDLLDAVL 253
           RIVDE+RRFW EE+Y+PGIPIDEIPDLNSCLLYQR QVINCC+SRKRRHEIATD +DA +
Sbjct: 282 RIVDEMRRFWSEEQYLPGIPIDEIPDLNSCLLYQRLQVINCCVSRKRRHEIATDSIDAAI 341

Query: 254 RVASSNTELRISEVTVPTNSLLYARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQE 313
           R ASSN E   S+VT+P N+LLYARLNNGELALR+GADC F + KMLETGE VYSPVTQE
Sbjct: 342 REASSNAESGTSKVTIPGNTLLYARLNNGELALRLGADCPFGDHKMLETGEAVYSPVTQE 401

Query: 314 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 373
           GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP
Sbjct: 402 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 461

Query: 374 EPSNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILN 433
           EPSN SIDS +G+DSRGQLSSRMQKEGNLW ELWETSKPVPAVKQTPLFDEDL VEGILN
Sbjct: 462 EPSNDSIDSPVGSDSRGQLSSRMQKEGNLWLELWETSKPVPAVKQTPLFDEDLVVEGILN 521

Query: 434 DLEDLPPSELYEPLFLSLLGLGFIMAEAKLAKDNDLSKMFYECKDYVVATCQGSFWSNKV 493
           DLEDLPPSEL+EPLF+SLLGLG IMAEAKLA +N+LSK+FY+CK YVVATCQ S WSNKV
Sbjct: 522 DLEDLPPSELFEPLFISLLGLGLIMAEAKLANNNNLSKLFYDCKGYVVATCQSSSWSNKV 581

Query: 494 DDICQVYETVETMMVNPAEILNAMKQPEESNMTASELKRRLR 536
           DD+CQVYETVETMMVNP EIL AMKQPEESNMTASELKRR +
Sbjct: 582 DDLCQVYETVETMMVNPEEILKAMKQPEESNMTASELKRRFK 620

BLAST of Sed0002781 vs. ExPASy TrEMBL
Match: A0A1S3BLC4 (Rab3 GTPase-activating protein catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103491073 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.8e-240
Identity = 420/522 (80.46%), Postives = 465/522 (89.08%), Query Frame = 0

Query: 14  NPTMSLPFYDDNLYILNMKNDIEAKNEEVVPSLEHLLATDEATIPPISAVKQLAVAVRAA 73
           NPTM++PFYD+NLY+LNMKNDIEAKN E++PS+E L  TD  +IPP+S +KQLA AV AA
Sbjct: 102 NPTMAVPFYDENLYLLNMKNDIEAKNAEIIPSVESLWTTDTDSIPPLSVIKQLATAVEAA 161

Query: 74  KKSKSLKTLLASASSSPHSSPAKDKSGLSLSAVRAFMLPEKEAKVSTAFGDDDRVQALIR 133
           KKSKS+K+LLA   SS  SSPA++KSGLSLS+VRA ML EKE K ST F  D+R+Q+LI 
Sbjct: 162 KKSKSMKSLLA---SSGDSSPAREKSGLSLSSVRALMLREKEEKSSTEFHHDERIQSLIC 221

Query: 134 SLFHADGVFLRRTLTSASDHTILTSLPRDIHGAPPHSFLVKISEVIGSFRTLRKMTLFWC 193
           SLF A+G+FLRR   +A + TI+TSLP+DIHGAPP S LVKISEVIGSFRTLRKM LFWC
Sbjct: 222 SLFDAEGIFLRRNFGTALEDTIVTSLPKDIHGAPPDSLLVKISEVIGSFRTLRKMALFWC 281

Query: 194 RIVDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIATDLLDAVL 253
           RIVDE+RRFW EE+Y+PGIPIDEIPDLNSCLLYQR QVINCC+SRKRRHEIATD +DA +
Sbjct: 282 RIVDEMRRFWSEEQYLPGIPIDEIPDLNSCLLYQRLQVINCCVSRKRRHEIATDSIDAAI 341

Query: 254 RVASSNTELRISEVTVPTNSLLYARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQE 313
           R ASSN E   S+VT+P N+LLYARLNNGELALR+GADC F + KMLETGE VYSPVTQE
Sbjct: 342 REASSNAESGTSKVTIPGNTLLYARLNNGELALRLGADCPFGDHKMLETGEAVYSPVTQE 401

Query: 314 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 373
           GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP
Sbjct: 402 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 461

Query: 374 EPSNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILN 433
           EPSN SIDS +G+DSRGQLSSRMQKEGNLW ELWETSKPVPAVKQTPLFDEDL VEGILN
Sbjct: 462 EPSNDSIDSPVGSDSRGQLSSRMQKEGNLWLELWETSKPVPAVKQTPLFDEDLVVEGILN 521

Query: 434 DLEDLPPSELYEPLFLSLLGLGFIMAEAKLAKDNDLSKMFYECKDYVVATCQGSFWSNKV 493
           DLEDLPPSEL+EPLF+SLLGLG IMAEAKLA +N+LSK+FY+CK YVVATCQ S WSNKV
Sbjct: 522 DLEDLPPSELFEPLFISLLGLGLIMAEAKLANNNNLSKLFYDCKGYVVATCQSSSWSNKV 581

Query: 494 DDICQVYETVETMMVNPAEILNAMKQPEESNMTASELKRRLR 536
           DD+CQVYETVETMMVNP EIL AMKQPEESNMTASELKRR +
Sbjct: 582 DDLCQVYETVETMMVNPEEILKAMKQPEESNMTASELKRRFK 620

BLAST of Sed0002781 vs. ExPASy TrEMBL
Match: A0A6J1KYY7 (Rab3 GTPase-activating protein catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111498379 PE=3 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 3.0e-240
Identity = 422/519 (81.31%), Postives = 464/519 (89.40%), Query Frame = 0

Query: 17  MSLPFYDDNLYILNMKNDIEAKNEEVVPSLEHLLATDEATIPPISAVKQLAVAVRAAKKS 76
           MS+PFYD+NLY+LNMKNDIEAKN E++PS+E  LATD+A+IP +S +KQLA+A+ A KKS
Sbjct: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60

Query: 77  KSLKTLLASASSSPHSSPAKDKSGLSLSAVRAFMLPEKEAKVSTAFGDDDRVQALIRSLF 136
           KS+K+LLAS++    SS AK K GL+LS VRA +L EKE K+ST F  D+R+Q+LIRSLF
Sbjct: 61  KSVKSLLASSAG---SSSAKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLF 120

Query: 137 HADGVFLRRTLTSASDHTILTSLPRDIHGAPPHSFLVKISEVIGSFRTLRKMTLFWCRIV 196
            A G FL+R++TS S+ TI  SLPRDIHGAPP S LVKISEVIGSFR+LRKMTLFWCRIV
Sbjct: 121 DAGGDFLKRSITSGSEGTIAASLPRDIHGAPPESLLVKISEVIGSFRSLRKMTLFWCRIV 180

Query: 197 DEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIATDLLDAVLRVA 256
           DE+RRFW EE+YIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIA+D LD VLRVA
Sbjct: 181 DEMRRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVA 240

Query: 257 SSNTELRISEVTVPTNSLLYARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQEGPL 316
           SSNTE   SEVTVP NSLLYARLNNGELALR+GADC F+NL MLETGE VYSPVTQEGPL
Sbjct: 241 SSNTESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPVTQEGPL 300

Query: 317 LTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPS 376
           LTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPS
Sbjct: 301 LTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPS 360

Query: 377 NGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILNDLE 436
           NGS+ SSI +DSRGQLSSRMQKEGNLWRELW+TSK VPAVKQTPLFDEDL VEGIL DLE
Sbjct: 361 NGSVHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLE 420

Query: 437 DLPPSELYEPLFLSLLGLGFIMAEAKLAKDNDLSKMFYECKDYVVATCQGSFWSNKVDDI 496
           DLP +ELY PLFLSLLGLGF+MAEAKL +DN+LSK+FYECKDYVVATCQGS W+NKVDDI
Sbjct: 421 DLPTTELYGPLFLSLLGLGFVMAEAKLTEDNNLSKLFYECKDYVVATCQGSSWNNKVDDI 480

Query: 497 CQVYETVETMMVNPAEILNAMKQPEESNMTASELKRRLR 536
           CQVYETVETMMVNP EIL AMKQ EESNMT S LKRR +
Sbjct: 481 CQVYETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFK 516

BLAST of Sed0002781 vs. ExPASy TrEMBL
Match: A0A6J1DCM4 (Rab3 GTPase-activating protein catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111019274 PE=3 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 1.1e-239
Identity = 422/522 (80.84%), Postives = 465/522 (89.08%), Query Frame = 0

Query: 14  NPTMSLPFYDDNLYILNMKNDIEAKNEEVVPSLEHLLATDEATIPPISAVKQLAVAVRAA 73
           NPTMS+PFYD+NLY+LNMKNDIEAKN EV+PS+E  LA D  +IPP S +KQLAVAV A 
Sbjct: 104 NPTMSVPFYDENLYLLNMKNDIEAKNAEVIPSVESFLAIDRTSIPPFSVIKQLAVAVEAG 163

Query: 74  KKSKSLKTLLASASSSPHSSPAKDKSGLSLSAVRAFMLPEKEAKVSTAFGDDDRVQALIR 133
           KK KS+K+LLA   SS  SS  ++KSGLSLSAVRA ++ EKE K+ST F  D R+Q+LI 
Sbjct: 164 KKCKSMKSLLA---SSGDSSSTREKSGLSLSAVRALVVREKEEKISTEFHHDARIQSLIC 223

Query: 134 SLFHADGVFLRRTLTSASDHTILTSLPRDIHGAPPHSFLVKISEVIGSFRTLRKMTLFWC 193
           SLF A+G FL+R+  SAS+ TI+TSLPRDIHGAPP S LVKISEVIGSFRTLRKMTLFWC
Sbjct: 224 SLFDAEGDFLKRSFGSASEGTIVTSLPRDIHGAPPDSLLVKISEVIGSFRTLRKMTLFWC 283

Query: 194 RIVDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIATDLLDAVL 253
           R+VDE+RRFW EE+YIPGIPIDEIPDLNSCLLYQR QVINCC+SRKRRHEIATD LDAV+
Sbjct: 284 RVVDEMRRFWSEEQYIPGIPIDEIPDLNSCLLYQRLQVINCCVSRKRRHEIATDSLDAVV 343

Query: 254 RVASSNTELRISEVTVPTNSLLYARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQE 313
           R ASSN E   SE TVP NS+LYA+LNNGEL+LR+GADC F +LKMLETGE VYSPVTQE
Sbjct: 344 R-ASSNAESGASEDTVPANSVLYAKLNNGELSLRLGADCPFGDLKMLETGEAVYSPVTQE 403

Query: 314 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 373
           GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP
Sbjct: 404 GPLLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEP 463

Query: 374 EPSNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILN 433
           +PS+ SIDS +G+DSRGQLSSRMQKEGNLWRELWETSK VPAVKQTPLFDEDL VEGIL+
Sbjct: 464 DPSSDSIDSPVGSDSRGQLSSRMQKEGNLWRELWETSKAVPAVKQTPLFDEDLVVEGILH 523

Query: 434 DLEDLPPSELYEPLFLSLLGLGFIMAEAKLAKDNDLSKMFYECKDYVVATCQGSFWSNKV 493
           DLEDLPPSEL+EPLF+SLLGLGFIMAE KL K+N+LSK+FYECKDY VATCQGSFWSNKV
Sbjct: 524 DLEDLPPSELFEPLFISLLGLGFIMAEEKLGKNNNLSKLFYECKDYAVATCQGSFWSNKV 583

Query: 494 DDICQVYETVETMMVNPAEILNAMKQPEESNMTASELKRRLR 536
           DDICQVYETVETMM+NP EIL  MKQPEESNMTASELKRR +
Sbjct: 584 DDICQVYETVETMMLNPEEILKTMKQPEESNMTASELKRRFK 621

BLAST of Sed0002781 vs. TAIR 10
Match: AT5G55060.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G58510.1); Has 223 Blast hits to 218 proteins in 80 species: Archae - 0; Bacteria - 2; Metazoa - 117; Fungi - 6; Plants - 65; Viruses - 0; Other Eukaryotes - 33 (source: NCBI BLink). )

HSP 1 Score: 617.8 bits (1592), Expect = 7.9e-177
Identity = 317/520 (60.96%), Postives = 398/520 (76.54%), Query Frame = 0

Query: 17  MSLPFYDDNLYILNMKNDIEAKNEEVVPSLEHLLATDEATIPPISAVKQLAVAVRAAKKS 76
           M+ PFYD+N YIL  K + EAK  +V   +E L A D  +IP  S VKQLAVA+ A K++
Sbjct: 94  MAAPFYDENFYILKAKQEQEAKASDVGCMVESLNAVDANSIPLASVVKQLAVAIEAGKRA 153

Query: 77  KSLKTLLASASSSPHSSPAKDKSGLSLSAVRAFMLPEKEAKVSTAFGDDDRVQALIRSLF 136
           K++K  +AS+ S   SSP +++ GLSLSAV++ +L EKE K+    GD+D++ +LI +LF
Sbjct: 154 KNVKDFVASSGS---SSPVRERGGLSLSAVKSLVLGEKEDKLGFDSGDEDKLVSLINALF 213

Query: 137 HADGVFLRRTLTS-ASDHTILTSLPRDIHGAPPHSFLVKISEVIGSFRTLRKMTLFWCRI 196
           + D  FL R + S     T  +S  +D+H APP SF+VK++EVIGSF T R+M LFWCR+
Sbjct: 214 NVDSGFLSRKIVSDLESPTNRSSFAKDLHAAPPSSFVVKLAEVIGSFTTPRRMALFWCRV 273

Query: 197 VDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIATDLLDAVLRV 256
           V+E+RRFW EE++IP IP+D  PDL SCLL+Q  QVINCCL+RK R+  A++ LDAV+R 
Sbjct: 274 VEELRRFWNEEKHIPWIPLDNNPDLKSCLLHQWLQVINCCLARKARNVAASEALDAVMRQ 333

Query: 257 ASSNTELRISEVTVPTNSLLYARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQEGP 316
           ASS  E   S+V+    SLLYA+ N+GEL LR+G   +  NL MLETGE VYSP+TQEGP
Sbjct: 334 ASSANEK--SDVSESMGSLLYAKSNSGELILRLGVYNQVENLTMLETGEPVYSPITQEGP 393

Query: 317 LLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEP 376
           LLTED+I+ETEE VLRTGS+GAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTE   
Sbjct: 394 LLTEDLIRETEELVLRTGSMGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTE--- 453

Query: 377 SNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILNDL 436
            NG++     +  RGQLS+RMQKEGNLWRELWET+KP+PAVKQ PLFDEDLAVEGILN L
Sbjct: 454 -NGNMSGDDSSPVRGQLSTRMQKEGNLWRELWETAKPLPAVKQAPLFDEDLAVEGILNYL 513

Query: 437 EDLPPSELYEPLFLSLLGLGFIMAEAKLAKDNDLSKMFYECKDYVVATCQGSFWSNKVDD 496
           ED+P +EL+E LF SL+ LGF+M E  LA ++DLSK+F+ECKDYVVA CQG  W++K+DD
Sbjct: 514 EDIPAAELFEQLFTSLVSLGFVMVEPVLATNDDLSKLFFECKDYVVAICQGGAWTDKLDD 573

Query: 497 ICQVYETVETMMVNPAEILNAMKQPEESNMTASELKRRLR 536
           +CQVYETVETM++ P E+L +MKQ EES  + SE KRR +
Sbjct: 574 LCQVYETVETMLLRPEEVLRSMKQTEESPSSGSETKRRFK 604

BLAST of Sed0002781 vs. TAIR 10
Match: AT5G55060.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G58510.1). )

HSP 1 Score: 617.8 bits (1592), Expect = 7.9e-177
Identity = 317/520 (60.96%), Postives = 398/520 (76.54%), Query Frame = 0

Query: 17  MSLPFYDDNLYILNMKNDIEAKNEEVVPSLEHLLATDEATIPPISAVKQLAVAVRAAKKS 76
           M+ PFYD+N YIL  K + EAK  +V   +E L A D  +IP  S VKQLAVA+ A K++
Sbjct: 1   MAAPFYDENFYILKAKQEQEAKASDVGCMVESLNAVDANSIPLASVVKQLAVAIEAGKRA 60

Query: 77  KSLKTLLASASSSPHSSPAKDKSGLSLSAVRAFMLPEKEAKVSTAFGDDDRVQALIRSLF 136
           K++K  +AS+ S   SSP +++ GLSLSAV++ +L EKE K+    GD+D++ +LI +LF
Sbjct: 61  KNVKDFVASSGS---SSPVRERGGLSLSAVKSLVLGEKEDKLGFDSGDEDKLVSLINALF 120

Query: 137 HADGVFLRRTLTS-ASDHTILTSLPRDIHGAPPHSFLVKISEVIGSFRTLRKMTLFWCRI 196
           + D  FL R + S     T  +S  +D+H APP SF+VK++EVIGSF T R+M LFWCR+
Sbjct: 121 NVDSGFLSRKIVSDLESPTNRSSFAKDLHAAPPSSFVVKLAEVIGSFTTPRRMALFWCRV 180

Query: 197 VDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIATDLLDAVLRV 256
           V+E+RRFW EE++IP IP+D  PDL SCLL+Q  QVINCCL+RK R+  A++ LDAV+R 
Sbjct: 181 VEELRRFWNEEKHIPWIPLDNNPDLKSCLLHQWLQVINCCLARKARNVAASEALDAVMRQ 240

Query: 257 ASSNTELRISEVTVPTNSLLYARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQEGP 316
           ASS  E   S+V+    SLLYA+ N+GEL LR+G   +  NL MLETGE VYSP+TQEGP
Sbjct: 241 ASSANEK--SDVSESMGSLLYAKSNSGELILRLGVYNQVENLTMLETGEPVYSPITQEGP 300

Query: 317 LLTEDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEP 376
           LLTED+I+ETEE VLRTGS+GAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTE   
Sbjct: 301 LLTEDLIRETEELVLRTGSMGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTE--- 360

Query: 377 SNGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILNDL 436
            NG++     +  RGQLS+RMQKEGNLWRELWET+KP+PAVKQ PLFDEDLAVEGILN L
Sbjct: 361 -NGNMSGDDSSPVRGQLSTRMQKEGNLWRELWETAKPLPAVKQAPLFDEDLAVEGILNYL 420

Query: 437 EDLPPSELYEPLFLSLLGLGFIMAEAKLAKDNDLSKMFYECKDYVVATCQGSFWSNKVDD 496
           ED+P +EL+E LF SL+ LGF+M E  LA ++DLSK+F+ECKDYVVA CQG  W++K+DD
Sbjct: 421 EDIPAAELFEQLFTSLVSLGFVMVEPVLATNDDLSKLFFECKDYVVAICQGGAWTDKLDD 480

Query: 497 ICQVYETVETMMVNPAEILNAMKQPEESNMTASELKRRLR 536
           +CQVYETVETM++ P E+L +MKQ EES  + SE KRR +
Sbjct: 481 LCQVYETVETMLLRPEEVLRSMKQTEESPSSGSETKRRFK 511

BLAST of Sed0002781 vs. TAIR 10
Match: AT5G58510.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G55060.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 146.0 bits (367), Expect = 8.8e-35
Identity = 109/387 (28.17%), Postives = 174/387 (44.96%), Query Frame = 0

Query: 185 LRKMTLFWCRIVDEIRRFWFEERYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEI 244
           +R +   W   V E+R  W E + +P +PID   DL+SCL+ Q+  ++  C+ +KR  E+
Sbjct: 439 IRAIAFLWIEFVREVRWCWEETQPLPKMPIDGSIDLSSCLINQKLHLLAICIEKKR--EM 498

Query: 245 ATDLLDAVLRVASSNT------------------------------------ELRISEVT 304
             + LD +    SS+                                     +LR    T
Sbjct: 499 NEEFLDCIGSDDSSDASVSMEEHHKVDKRRNTSSEEELRRKRDSSIAEDTSKQLRFERKT 558

Query: 305 VPTNSLLYARLNNGELALRIGADCEFTNLKMLETGETVYSPVTQEGPLLTEDVIKETEEF 364
             TNS+  +  +    A+R G+      + +L++ + +++P TQ+ PL+TED+ +E  + 
Sbjct: 559 ERTNSVNQSPTD----AIRRGSAGPVGTMMLLKSRQQLHAPFTQDPPLMTEDMHEERLQA 618

Query: 365 VLRTGSVGAGCSQ-----LLSDMQAFKAANPGCILEDFVRWHSPPDW-------TEPEPS 424
           V   G       Q     LLSDM AFKAANP  + EDF+RWHSP DW       TEP   
Sbjct: 619 VEAFGDSLNVPGQLEKDILLSDMSAFKAANPDAVFEDFIRWHSPGDWESFEPKTTEPSAG 678

Query: 425 NGSIDSSIGNDSRGQLSSRMQKEGNLWRELWETSKPVPAVKQTPLFDEDLAVEGILNDLE 484
             +  S      RG+LS RM  +GNLWR+ W  +  +PA  Q PL D +   E I++ LE
Sbjct: 679 PSTEGSKDEWPPRGRLSQRMSDQGNLWRKSWNDAPALPADDQKPLLDPNREGEKIVHYLE 738

Query: 485 DLPPSELYEPLFLSLL-GLGFIMAEAKLAKDNDLS---KMFYECKDYVVATCQGSFWSNK 514
            + P +L E +  +   G    + +  +     ++   +  Y    + +   Q +   +K
Sbjct: 739 TVRPHQLLEQMVCTAFRGSADTLNQTNVGNMRQMTSKLEQLYLIMKFTLGALQRNNLPDK 798