Sed0002543 (gene) Chayote v1

BLAST of Sed0002543 vs. NCBI nr
Match: XP_023542995.1 (uncharacterized protein LOC111802746 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 833.2 bits (2151), Expect = 1.4e-237
Identity = 426/550 (77.45%), Postives = 454/550 (82.55%), Query Frame = 0

Query: 56  LNSDAAADLVVKNTLIFTSDGSLPFADSMAVLNRRILRIGTYSALQDLVGQGTKELNLGG 115
           L+S A ADLVVKN LIFTSD SLPFADSMA+LNRRILR+GTYSA+QDLVGQGTKELNLGG
Sbjct: 5   LSSCAVADLVVKNALIFTSDDSLPFADSMAILNRRILRVGTYSAVQDLVGQGTKELNLGG 64

Query: 116 KIVVPGFIDSHGHLIYQGLQMKQVNLHGINNKSELVRRIGEAARNTKKGNWVLGGGWNSD 175
           KIVVPGFIDSHGHLIYQGLQMKQVNLHG+N+KSE VRRI EAA+N KKGNWVLGGGWN+D
Sbjct: 65  KIVVPGFIDSHGHLIYQGLQMKQVNLHGVNHKSEFVRRIAEAAKNAKKGNWVLGGGWNND 124

Query: 176 -----LPVKLMV-----------------MSLANNVTLKLAGVTNLTENPEGGTVVKTTD 235
                LP+   +                 MSLANNVTLKLAG+TNLTE+PEGGTVVKT  
Sbjct: 125 LWGGELPMASWIDDVTPSNPVLVSRIDGHMSLANNVTLKLAGITNLTEDPEGGTVVKTIG 184

Query: 236 GDPTGLLVDSARKLVLPFIPEVTVEERREALESVSNLALSRGVTTIVDFGRYYPAESAKL 295
           GDPTG+L+DSARKLVLPFIPEVTVEERREAL  VS LAL+RGVTTI+DFGRYYP ES KL
Sbjct: 185 GDPTGVLIDSARKLVLPFIPEVTVEERREALIRVSKLALARGVTTILDFGRYYPGESVKL 244

Query: 296 SWEDFSDVYQWADSSGKMKIRVCLFFPMETWSSLHDLNQKMGQVVSPWIYLGGVKGFADG 355
           SWEDFSDVYQWADSSGKM IRVCLFFPMETWS LHDL  KMGQVVSPW+YLGGVKGFADG
Sbjct: 245 SWEDFSDVYQWADSSGKMMIRVCLFFPMETWSYLHDLIHKMGQVVSPWVYLGGVKGFADG 304

Query: 356 SLGSHTALFH-----EPDNCGIQMTERENLFNMSMESDKSKLQVAIHAIGDKANDMIVDI 415
           SLGSHTALFH     EPDNCG+QMTERENLFNM+MESDKSKLQVAIHAIGDKANDMI+DI
Sbjct: 305 SLGSHTALFHEPYVDEPDNCGMQMTERENLFNMTMESDKSKLQVAIHAIGDKANDMILDI 364

Query: 416 YESIILTNGPRDRIFRVMSCSTLAPGAPERFGKLGTIASAQPEHLLDDTESATNKLGPQR 475
           YES+I TNGPRDR FRV     LAPGAPERFG LG IASAQPEHLLDD ESATNKLG QR
Sbjct: 365 YESVISTNGPRDRRFRVEHAQHLAPGAPERFGNLGIIASAQPEHLLDDAESATNKLGAQR 424

Query: 476 AQKESFLFRSLLTCKACLAFGSDCPIYNSFLCVDRKYQSIGWYQDRNEKEPPTWDHAWIP 535
           AQKESFLFRSLLTCKACLAFGSDCP+ N           +G  +    + PPTWDHAWIP
Sbjct: 425 AQKESFLFRSLLTCKACLAFGSDCPVAN--------INPLGGIKTALRRIPPTWDHAWIP 484

Query: 536 SECLELDEALKAYTISVAYASFLDKDLGSLSPGKLADFVVLFYRFMGRVRKQKDPLPSEA 579
           SECL LDEA+KAYTIS AYASFLDKDLGSLSPGKLADFV+L          +      EA
Sbjct: 485 SECLTLDEAIKAYTISAAYASFLDKDLGSLSPGKLADFVILSTNSWDEFAAE-GSASIEA 544

BLAST of Sed0002543 vs. NCBI nr
Match: XP_022945604.1 (uncharacterized protein LOC111449791 isoform X1 [Cucurbita moschata])

HSP 1 Score: 832.8 bits (2150), Expect = 1.8e-237
Identity = 426/549 (77.60%), Postives = 453/549 (82.51%), Query Frame = 0

Query: 57  NSDAAADLVVKNTLIFTSDGSLPFADSMAVLNRRILRIGTYSALQDLVGQGTKELNLGGK 116
           +S A ADLVVKN LIFTSD SLPFADSMA+ NRRILR+GTYSA+QDLVGQGTKELNLGGK
Sbjct: 57  SSCAVADLVVKNALIFTSDDSLPFADSMAIRNRRILRVGTYSAVQDLVGQGTKELNLGGK 116

Query: 117 IVVPGFIDSHGHLIYQGLQMKQVNLHGINNKSELVRRIGEAARNTKKGNWVLGGGWNSD- 176
           IVVPGFIDSHGHLIYQGLQMKQVNLHG+N+KSE VRRI EAA+N KKGNWVLGGGWN+D 
Sbjct: 117 IVVPGFIDSHGHLIYQGLQMKQVNLHGVNHKSEFVRRIAEAAKNAKKGNWVLGGGWNNDL 176

Query: 177 ----LPVKLMV-----------------MSLANNVTLKLAGVTNLTENPEGGTVVKTTDG 236
               LP+   +                 MSLANNVTLKLAG+TNLTE+PEGGTVVKT  G
Sbjct: 177 WGGELPMASWIDDVTPSNPVLVSRIDGHMSLANNVTLKLAGITNLTEDPEGGTVVKTIGG 236

Query: 237 DPTGLLVDSARKLVLPFIPEVTVEERREALESVSNLALSRGVTTIVDFGRYYPAESAKLS 296
           DPTG+L+DSARKLVLPFIPEVTVEERREAL  VS LAL+RGVTTIVDFGRYYP ES KLS
Sbjct: 237 DPTGVLIDSARKLVLPFIPEVTVEERREALIRVSKLALARGVTTIVDFGRYYPGESVKLS 296

Query: 297 WEDFSDVYQWADSSGKMKIRVCLFFPMETWSSLHDLNQKMGQVVSPWIYLGGVKGFADGS 356
           WEDFSDVYQWADSSGKM IRVCLFFPMETWS LHDL  KMGQVVSPW+YLGGVKGFADGS
Sbjct: 297 WEDFSDVYQWADSSGKMMIRVCLFFPMETWSYLHDLIHKMGQVVSPWVYLGGVKGFADGS 356

Query: 357 LGSHTALFH-----EPDNCGIQMTERENLFNMSMESDKSKLQVAIHAIGDKANDMIVDIY 416
           LGSHTALFH     EPDNCG+QMTERENLFNM+MESDKSKLQVAIHAIGDKANDMI+DIY
Sbjct: 357 LGSHTALFHEPYVDEPDNCGMQMTERENLFNMTMESDKSKLQVAIHAIGDKANDMILDIY 416

Query: 417 ESIILTNGPRDRIFRVMSCSTLAPGAPERFGKLGTIASAQPEHLLDDTESATNKLGPQRA 476
           ES+I TNGPRDR FRV     LAPGAPERFGKLG IASAQPEHLLDD ESATNKLG QRA
Sbjct: 417 ESVISTNGPRDRRFRVEHAQHLAPGAPERFGKLGIIASAQPEHLLDDAESATNKLGAQRA 476

Query: 477 QKESFLFRSLLTCKACLAFGSDCPIYNSFLCVDRKYQSIGWYQDRNEKEPPTWDHAWIPS 536
           QKESFLFRSLLTCKACLAFGSDCP+ N           +G  +    + PPTWDHAWIPS
Sbjct: 477 QKESFLFRSLLTCKACLAFGSDCPVAN--------INPLGGIKTALRRIPPTWDHAWIPS 536

Query: 537 ECLELDEALKAYTISVAYASFLDKDLGSLSPGKLADFVVLFYRFMGRVRKQKDPLPSEAT 579
           ECL LDEA+KAYTIS AYASFLDKDLGSLSPGKLADFV+L          +      EAT
Sbjct: 537 ECLTLDEAIKAYTISAAYASFLDKDLGSLSPGKLADFVILSTNSWDEFAAE-GSASIEAT 596

BLAST of Sed0002543 vs. NCBI nr
Match: XP_022967005.1 (uncharacterized protein LOC111466536 [Cucurbita maxima])

HSP 1 Score: 832.4 bits (2149), Expect = 2.4e-237
Identity = 427/549 (77.78%), Postives = 452/549 (82.33%), Query Frame = 0

Query: 57  NSDAAADLVVKNTLIFTSDGSLPFADSMAVLNRRILRIGTYSALQDLVGQGTKELNLGGK 116
           +S A ADLVVKN LIFTSD SLPFADSM VLNRRILR+GTYSA+QDLVGQGTKELNLGGK
Sbjct: 6   SSCAVADLVVKNALIFTSDDSLPFADSMTVLNRRILRVGTYSAVQDLVGQGTKELNLGGK 65

Query: 117 IVVPGFIDSHGHLIYQGLQMKQVNLHGINNKSELVRRIGEAARNTKKGNWVLGGGWNSD- 176
           IVVPGFIDSHGHLIYQGLQMKQVNLHG+N+KSE VRRI EAA+N KKGNWVLGGGWN+D 
Sbjct: 66  IVVPGFIDSHGHLIYQGLQMKQVNLHGVNHKSEFVRRIAEAAKNAKKGNWVLGGGWNNDL 125

Query: 177 ----LPVKLMV-----------------MSLANNVTLKLAGVTNLTENPEGGTVVKTTDG 236
               LP+   +                 MSLANNVTLKLAG+TNLTE+PEGGTVVKT  G
Sbjct: 126 WGGELPMASWIDDVTPSNPVLVSRIDGHMSLANNVTLKLAGITNLTEDPEGGTVVKTIGG 185

Query: 237 DPTGLLVDSARKLVLPFIPEVTVEERREALESVSNLALSRGVTTIVDFGRYYPAESAKLS 296
           DPTG+L+DSARKLVLPFIPEVTVEERREAL  VS LAL+RGVTTIVDFGRYYP ES KLS
Sbjct: 186 DPTGVLIDSARKLVLPFIPEVTVEERREALIRVSKLALARGVTTIVDFGRYYPGESVKLS 245

Query: 297 WEDFSDVYQWADSSGKMKIRVCLFFPMETWSSLHDLNQKMGQVVSPWIYLGGVKGFADGS 356
           WEDFSDVYQWADSSGKM IRVCLFFPMETWS LHDL  KMGQVVSPW+YLGGVKGFADGS
Sbjct: 246 WEDFSDVYQWADSSGKMMIRVCLFFPMETWSYLHDLIHKMGQVVSPWVYLGGVKGFADGS 305

Query: 357 LGSHTALFH-----EPDNCGIQMTERENLFNMSMESDKSKLQVAIHAIGDKANDMIVDIY 416
           LGSHTALFH     EPDNCG+QMTERENLFNM+MESDKSKLQVAIHAIGDKANDMI+DIY
Sbjct: 306 LGSHTALFHEPYVDEPDNCGMQMTERENLFNMTMESDKSKLQVAIHAIGDKANDMILDIY 365

Query: 417 ESIILTNGPRDRIFRVMSCSTLAPGAPERFGKLGTIASAQPEHLLDDTESATNKLGPQRA 476
           ES+I TNGPRDR FRV     LAPGAPERFGKLG IASAQPEHLLDD ESATNKLG QRA
Sbjct: 366 ESVISTNGPRDRRFRVEHAQHLAPGAPERFGKLGIIASAQPEHLLDDAESATNKLGAQRA 425

Query: 477 QKESFLFRSLLTCKACLAFGSDCPIYNSFLCVDRKYQSIGWYQDRNEKEPPTWDHAWIPS 536
           QKESFLFRSLLTCKACLAFGSDCPI N           +G  +    + PPTWDHAWIPS
Sbjct: 426 QKESFLFRSLLTCKACLAFGSDCPIAN--------INPLGGIKTALRRIPPTWDHAWIPS 485

Query: 537 ECLELDEALKAYTISVAYASFLDKDLGSLSPGKLADFVVLFYRFMGRVRKQKDPLPSEAT 579
           ECL LDEA+KAYTIS AYASFLDKDLGSLSPGKLADFV+L          +      EAT
Sbjct: 486 ECLTLDEAIKAYTISAAYASFLDKDLGSLSPGKLADFVILSTNSWDEFAAE-GSASIEAT 545

BLAST of Sed0002543 vs. NCBI nr
Match: KAG7012876.1 (ytcJ [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 829.7 bits (2142), Expect = 1.5e-236
Identity = 425/549 (77.41%), Postives = 452/549 (82.33%), Query Frame = 0

Query: 57  NSDAAADLVVKNTLIFTSDGSLPFADSMAVLNRRILRIGTYSALQDLVGQGTKELNLGGK 116
           +S A ADLVVKN LIFTSD SLPFADSMA+ NRRILR+GTYSA+QDLVGQGTKELNLGGK
Sbjct: 6   SSCAVADLVVKNALIFTSDDSLPFADSMAIRNRRILRVGTYSAVQDLVGQGTKELNLGGK 65

Query: 117 IVVPGFIDSHGHLIYQGLQMKQVNLHGINNKSELVRRIGEAARNTKKGNWVLGGGWNSD- 176
           IVVPGFIDSHGHLIYQGLQMKQVNLHG+N+KSE VRRI EAA+N KKGNWVLGGGWN+D 
Sbjct: 66  IVVPGFIDSHGHLIYQGLQMKQVNLHGVNHKSEFVRRIAEAAKNAKKGNWVLGGGWNNDL 125

Query: 177 ----LPVKLMV-----------------MSLANNVTLKLAGVTNLTENPEGGTVVKTTDG 236
               LP+   +                 MSLANNVTLKLAG+TNLTE+PEGGTVVKT  G
Sbjct: 126 WGGELPMASWIDDVTPSNPVLVSRIDGHMSLANNVTLKLAGITNLTEDPEGGTVVKTIGG 185

Query: 237 DPTGLLVDSARKLVLPFIPEVTVEERREALESVSNLALSRGVTTIVDFGRYYPAESAKLS 296
           DPTG+L+DSARKLVLPFIPEVTVEERREAL  VS LAL+RGVTTIVDFGRYYP ES KLS
Sbjct: 186 DPTGVLIDSARKLVLPFIPEVTVEERREALIRVSKLALARGVTTIVDFGRYYPGESVKLS 245

Query: 297 WEDFSDVYQWADSSGKMKIRVCLFFPMETWSSLHDLNQKMGQVVSPWIYLGGVKGFADGS 356
           WEDFSDVYQWADSSGKM IRVCLFFPMETWS LHDL  KMGQVVSPW+YLGGVKGFADGS
Sbjct: 246 WEDFSDVYQWADSSGKMMIRVCLFFPMETWSYLHDLIHKMGQVVSPWVYLGGVKGFADGS 305

Query: 357 LGSHTALFH-----EPDNCGIQMTERENLFNMSMESDKSKLQVAIHAIGDKANDMIVDIY 416
           LGSHTALFH     EPDNCG+QMTERENLFNM+MESDKSKLQVAIHAIGDKANDMI+DIY
Sbjct: 306 LGSHTALFHEPYVDEPDNCGMQMTERENLFNMTMESDKSKLQVAIHAIGDKANDMILDIY 365

Query: 417 ESIILTNGPRDRIFRVMSCSTLAPGAPERFGKLGTIASAQPEHLLDDTESATNKLGPQRA 476
           ES+I TNGPRDR FRV     LAPGAPERFGKLG IASAQPEHLLDD ESATNKLG QRA
Sbjct: 366 ESVISTNGPRDRRFRVEHAQHLAPGAPERFGKLGIIASAQPEHLLDDAESATNKLGAQRA 425

Query: 477 QKESFLFRSLLTCKACLAFGSDCPIYNSFLCVDRKYQSIGWYQDRNEKEPPTWDHAWIPS 536
           QKESFLFRSLLTCKACLAFGSDCP+ N           +G  +    + PPTWDHAWIPS
Sbjct: 426 QKESFLFRSLLTCKACLAFGSDCPVAN--------INPLGGIKTALRRIPPTWDHAWIPS 485

Query: 537 ECLELDEALKAYTISVAYASFLDKDLGSLSPGKLADFVVLFYRFMGRVRKQKDPLPSEAT 579
           ECL LDEA+KAYTIS AYASFLDKDLGSLSP KLADFV+L          +      EAT
Sbjct: 486 ECLTLDEAIKAYTISAAYASFLDKDLGSLSPRKLADFVILSTNSWDEFAAE-GSASIEAT 545

BLAST of Sed0002543 vs. NCBI nr
Match: XP_022139269.1 (uncharacterized protein LOC111010225 [Momordica charantia])

HSP 1 Score: 814.3 bits (2102), Expect = 6.7e-232
Identity = 422/549 (76.87%), Postives = 448/549 (81.60%), Query Frame = 0

Query: 57  NSDAAADLVVKNTLIFTSDGSLPFADSMAVLNRRILRIGTYSALQDLVGQGTKELNLGGK 116
           +S   ADLVVKN LIFTSD SLPFADSMA+LNRRILR+G+YSALQDLVGQGTKELNLGGK
Sbjct: 4   SSCLVADLVVKNALIFTSDDSLPFADSMAILNRRILRVGSYSALQDLVGQGTKELNLGGK 63

Query: 117 IVVPGFIDSHGHLIYQGLQMKQVNLHGINNKSELVRRIGEAARNTKKGNWVLGGGWNSDL 176
           IVVPGFIDSHGHLIYQGLQMKQVNLHG+N K+E VRRI EAARNT+KGNWVLGGGWN+DL
Sbjct: 64  IVVPGFIDSHGHLIYQGLQMKQVNLHGVNKKNEFVRRIAEAARNTEKGNWVLGGGWNNDL 123

Query: 177 ------------------PVKLM----VMSLANNVTLKLAGVTNLTENPEGGTVVKTTDG 236
                             PV L      MSLANNVTLKLAG+TNLTE+PEGGT+VKTT G
Sbjct: 124 WGGEWPMASWIDDVTASNPVLLSRIDGHMSLANNVTLKLAGITNLTEDPEGGTIVKTTGG 183

Query: 237 DPTGLLVDSARKLVLPFIPEVTVEERREALESVSNLALSRGVTTIVDFGRYYPAESAKLS 296
           DPTGLL+DSARKLVLPFIPEV+V+ERREAL  VS+LAL+RGVTTIVDFGRYYP ES KLS
Sbjct: 184 DPTGLLIDSARKLVLPFIPEVSVDERREALLRVSSLALARGVTTIVDFGRYYPGESVKLS 243

Query: 297 WEDFSDVYQWADSSGKMKIRVCLFFPMETWSSLHDLNQKMGQVVSPWIYLGGVKGFADGS 356
           WEDFSDVYQWADSS KM IRVCLFFPMETWSSLHDL  KMG VVSPWIYLGGVKGFADGS
Sbjct: 244 WEDFSDVYQWADSSEKMMIRVCLFFPMETWSSLHDLIHKMGHVVSPWIYLGGVKGFADGS 303

Query: 357 LGSHTALFH-----EPDNCGIQMTERENLFNMSMESDKSKLQVAIHAIGDKANDMIVDIY 416
           LGS TALFH     EPDN G+QMTERENLFNM+M SDKSKLQVAIHAIGDKANDM++DIY
Sbjct: 304 LGSRTALFHEPYVDEPDNYGMQMTERENLFNMTMASDKSKLQVAIHAIGDKANDMVLDIY 363

Query: 417 ESIILTNGPRDRIFRVMSCSTLAPGAPERFGKLGTIASAQPEHLLDDTESATNKLGPQRA 476
           ES+I TNGPRDR FRV     LA G PERFGKLG IASAQPEHLLDD ESATNKLG QRA
Sbjct: 364 ESVISTNGPRDRRFRVEHAQHLAHGTPERFGKLGIIASAQPEHLLDDAESATNKLGTQRA 423

Query: 477 QKESFLFRSLLTCKACLAFGSDCPIYNSFLCVDRKYQSIGWYQDRNEKEPPTWDHAWIPS 536
           QKESFLFRSLLTCKACLAFGSDCPI N           +G  +    + PPTWD AWIPS
Sbjct: 424 QKESFLFRSLLTCKACLAFGSDCPIAN--------INPMGGIKTAIRRTPPTWDRAWIPS 483

Query: 537 ECLELDEALKAYTISVAYASFLDKDLGSLSPGKLADFVVLFYRFMGRVRKQKDPLPSEAT 579
           ECL LDEALKAYTIS AYASFLDKDLGSLSPGKLADFV+L          Q      EAT
Sbjct: 484 ECLRLDEALKAYTISAAYASFLDKDLGSLSPGKLADFVILSTDSWDDFEAQ-GSASIEAT 543

BLAST of Sed0002543 vs. ExPASy Swiss-Prot
Match: A0A1I9LN01 (Protein LONG AFTER FAR-RED 3 OS=Arabidopsis thaliana OX=3702 GN=LAF3 PE=2 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 6.2e-156
Identity = 291/572 (50.87%), Postives = 382/572 (66.78%), Query Frame = 0

Query: 35  LIFLIVIPILHQSFTFEQESRL-NSDAAADLVVKNTLIFTSDGSLPFADSMAVLNRRILR 94
           LI +  +P+L+  +    +S    +   ADL+V N  IFTSD SLPFADSMA+ N RIL+
Sbjct: 21  LISVAYLPLLNDLYWSTLKSLTPPAGIVADLLVTNGTIFTSDSSLPFADSMAIRNGRILK 80

Query: 95  IGTYSALQDLVGQGTKELNLGGKIVVPGFIDSHGHLIYQGLQMKQVNLHGINNKSELVRR 154
           +G+++ L+  +G GT E+NL GKIVVPG IDSH HLI  GLQM QV L G++ K E  + 
Sbjct: 81  VGSFATLKGFIGDGTMEVNLEGKIVVPGLIDSHVHLISGGLQMAQVGLRGVSQKDEFCKM 140

Query: 155 IGEAARNTKKGNWVLGGGWNSDL------------------PVKLM----VMSLANNVTL 214
           + +A +N K+G+W+LGGGWN+D                   PV L+     M+LAN++ L
Sbjct: 141 VKDAVQNAKEGSWILGGGWNNDFWGGELPSASWIDEISPRNPVWLIRMDGHMALANSLAL 200

Query: 215 KLAGVTNLTENPEGGTVVKTTDGDPTGLLVDSARKLVLPFIPEVTVEERREALESVSNLA 274
           K+AGV +LTE+P GGT+++   G+PTGLL+D+A +LV P++ E++V+ERREAL   S  A
Sbjct: 201 KIAGVISLTEDPVGGTIMRMPSGEPTGLLIDAAMELVTPWVKEISVDERREALFRASKYA 260

Query: 275 LSRGVTTIVDFGRYYPAESAKLSWEDFSDVYQWADSSGKMKIRVCLFFPMETWSSLHDLN 334
           L+RGVTT++D GRY+P  + +LSW+DF DVY +ADSS KM IR CLFFP+ TWS L DL 
Sbjct: 261 LTRGVTTVIDLGRYFPGTTDELSWKDFQDVYLYADSSKKMMIRTCLFFPITTWSRLLDLK 320

Query: 335 QKMGQVVSPWIYLGGVKGFADGSLGSHTALFHE-----PDNCGIQMTERENLFNMSMESD 394
            + G V+S W+YLGGVK F DGSLGS++ALF+E     P+N G+++ + E L N +M +D
Sbjct: 321 LQKGSVLSEWLYLGGVKAFIDGSLGSNSALFYEEYIDTPNNYGLEVMDPEKLSNFTMAAD 380

Query: 395 KSKLQVAIHAIGDKANDMIVDIYESIILTNGPRDRIFRVMSCSTLAPGAPERFGKLGTIA 454
           KS LQVAIHAIGDKANDMI+D+YES+   NG RDR FR+     LAPG+  RFG+L  +A
Sbjct: 381 KSGLQVAIHAIGDKANDMILDMYESVAAANGDRDRRFRIEHAQHLAPGSANRFGQLHIVA 440

Query: 455 SAQPEHLLDDTESATNKLGPQRAQKESFLFRSLLTCKACLAFGSDCPIYNSFLCVDRKYQ 514
           S QP+HLLDD +S   KLG +RA KES+LF+SLL   A LA GSD P+ +          
Sbjct: 441 SVQPDHLLDDADSVAKKLGSERAVKESYLFQSLLNGNALLALGSDWPVAD--------IN 500

Query: 515 SIGWYQDRNEKEPPTWDHAWIPSECLELDEALKAYTISVAYASFLDKDLGSLSPGKLADF 574
            +   +   ++ PP WDHAWIPSE +   +AL A TIS A A+FLD  LGSLSPGKLADF
Sbjct: 501 PLHSIRTAVKRIPPKWDHAWIPSERISFTDALIAQTISAARAAFLDHHLGSLSPGKLADF 560

Query: 575 VVLFYRFMGRVRKQKDPLPSEATYTGGEQAYP 579
           V+L         K        ATY GG+Q YP
Sbjct: 561 VILSTNSWDEFSKDVS-ASVLATYVGGKQLYP 583

BLAST of Sed0002543 vs. ExPASy Swiss-Prot
Match: O34355 (Putative amidohydrolase YtcJ OS=Bacillus subtilis (strain 168) OX=224308 GN=ytcJ PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 3.6e-47
Identity = 146/495 (29.49%), Postives = 236/495 (47.68%), Query Frame = 0

Query: 95  GTYSALQDLVGQ-GTKELNLGGKIVVPGFIDSHGHLIYQGLQMKQVNLHGINNKSELVRR 154
           G+Y  L++  G   T+E++L G ++ PGF+DSH HLI  G +  Q++L  + +K  +++ 
Sbjct: 34  GSYERLKEKYGSPETEEISLNGAVMFPGFVDSHLHLIGHGEKQLQLDLSALTSKDSILQA 93

Query: 155 IGEAARNTKKGNWVLGGGWNS-------------------DLPVKLMVMS----LANNVT 214
             E  R   K +W++G GWN                    D PV L  +       N+  
Sbjct: 94  AKERERQLPKNDWLIGEGWNENQFETPDYLTKHDLDRLFPDRPVLLKRICRHAIAVNSAA 153

Query: 215 LKLAGVTNLTENPEGGTVVKTTDGDPTGLLVDSARKLVLPFIPEVTVEERREALESVSNL 274
           L+ AG++  T +P+GG +VK  +G+PTGLL D A+ L+L  +P V+     EAL +    
Sbjct: 154 LQAAGISRNTPDPDGGVIVKDANGEPTGLLFDKAQDLILKAVPPVSQHYVDEALTAAIKD 213

Query: 275 ALSRGVTTIVDFGRYYPAESAKLSWEDFSDV------YQWADSSGKMKIRVCLFF---PM 334
             ++G+T            S  LS+  + DV      Y+ A + GK   R  L      +
Sbjct: 214 CWTKGLT---------GGHSEDLSY--YGDVSVPMKAYEKAAAGGKYPFRCHLLVHHEAV 273

Query: 335 ETWSSLHDLNQKMGQVVSPWIYLGGVKGFADGSLGSHTALFHE-----PDNCGIQMTERE 394
           + W  L  L+        P++  G +K FADG+LG  TAL  E     P   G+Q+ + E
Sbjct: 274 DRWEQLEKLS-------GPYVEFGAMKIFADGALGGRTALLKEPYQDDPSTNGVQVHDDE 333

Query: 395 NLFNMSMESDKSKLQVAIHAIGDKANDMIVDIYESIILTNGPRDRIFRVMSCSTLAPGAP 454
            L  +  ++ +  ++VA+HAIGD A + +++  E     NG  DR+   +    L     
Sbjct: 334 TLGRLIRKAREKGMEVAVHAIGDLAFEKVLNAIEKHPPKNGRHDRL---IHAQVLDNELI 393

Query: 455 ERFGKLGTIASAQPEHLLDDTESATNKLGPQRAQKESFLFRSLLTCKACLAFGSDCPI-- 514
           ER  ++      QP  +  D     ++LG  R  K +F +++L++     A GSD PI  
Sbjct: 394 ERAARMPIALDLQPHFVASDFPWVIDRLGKDR-MKTAFAWKTLISKGILCAGGSDAPIEP 453

Query: 515 YNSFLCVDRKYQSIGWYQDRNEKEPPTWDHAWIPSECLELDEALKAYTISVAYASFLDKD 550
            +  L +    QS    +  +E+  P+++     SECL + EA+K YT   A   + +K 
Sbjct: 454 VDPLLGI----QSAVLRKSSHEQNGPSYNE----SECLPVYEAIKLYTEGSAGIIYKEKS 498

BLAST of Sed0002543 vs. ExPASy Swiss-Prot
Match: Q68AP4 (N-substituted formamide deformylase OS=Arthrobacter pascens OX=1677 GN=nfdA PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.5e-29
Identity = 138/526 (26.24%), Postives = 222/526 (42.21%), Query Frame = 0

Query: 63  DLVVKNTLIFTSDGSLPFADSMAVLNRRILRIGTYSALQDLVGQGTKELNLGGKIVVPGF 122
           DL++ N  + T D     A ++ V   RI  +GT + ++       + L++ GK VVPGF
Sbjct: 6   DLMIINANVRTVDARNSCAQAVLVSGGRIAIVGTETEVRGAAAPDAEVLDVSGKTVVPGF 65

Query: 123 IDSHGHLIYQGLQMKQVNLH--GINNKSELVRRIGEAARNTKKGNWVLGGGWNS------ 182
           ID+H HL         V+     +    E++  I    RN   G WV G  +++      
Sbjct: 66  IDAHNHLSVAAFAPDSVDCSTPPLATLDEVLEVIERHCRNIPPGQWVRGINFHASHIREQ 125

Query: 183 -------------DLPVKLMVMS----LANNVTLKLAGVTNLTENPEGGTVVKTTDGDPT 242
                        + P  L+  S     AN+  L L G+   T  P GG + +   G PT
Sbjct: 126 RNPTRYELDEVAPNNPFFLIDASCHAGFANSAALDLVGIGAHTPEPWGGEIERDLSGKPT 185

Query: 243 GLLVDSARKLVLPF----IPEVTVEERREALESVSNLALSRGVTTIVDFGRYYPAESAKL 302
           G L+++A  L+         E   +   E L S  N  L+ G+T + D      A+SA+L
Sbjct: 186 GTLLEAAANLLHSASWNDYAERDWDRAVELLHSKMNDYLAVGLTGVGD--AMVTAKSAEL 245

Query: 303 SWEDFSDVYQWADSSGKMKIRVCLFFPMETWSSLHDLNQK--MGQVVSPWIYL---GGVK 362
                   Y+ AD++GKM   +      + + S+ DL +   + +++ P  YL   G +K
Sbjct: 246 --------YRRADAAGKMPFTLQQLHGGDHFFSMQDLGRSDTVDRIMEPESYLLRGGAMK 305

Query: 363 GFADGSLGSHTALFHEPDNC----GIQMTERENLFNMSMESDKSKLQVAIHAIGDKANDM 422
            F D +  S  A+    D C    G     +  + ++++ + K  + +AIH +G+ A D+
Sbjct: 306 IFVDRAYPS-PAIDQIHDGCKTHVGANFYSKSEVHDLAVRASKLGINLAIHGMGNCAIDI 365

Query: 423 IVDIYESIILTNGPRDRIFRVMSCSTLAPGAPERFGKLGTIASAQPEHLLDDTESATNKL 482
           ++D YE+ +      D + R+        G  +R   LG    A P       E      
Sbjct: 366 VLDAYEA-VRRQSNADTVLRLEHAFIAETGQGQRMADLGIDLVANPGLAFGWGEVFNMWR 425

Query: 483 GPQRAQKESFLFRSLLTCKACLAFGSDCPIYNSFLCVDRKYQSIGWYQDRNEKEPPTWDH 542
           G  +   + F  RS+L     ++  SD P      C       I W     E    T   
Sbjct: 426 GENQEHLKLFPVRSMLDAGVRVSLASDHP------CGTYSPAEIMWTAVARE----TMAG 485

Query: 543 AWI-PSECLELDEALKAYTISVAYASFLDKDLGSLSPGKLADFVVL 550
           A + P E +  DEAL+ YTI+ A+AS    + GS+  GK A+ +VL
Sbjct: 486 APLEPDEAVTADEALRMYTINPAHASGRGSEEGSIEAGKRANLLVL 509

BLAST of Sed0002543 vs. ExPASy Swiss-Prot
Match: Q06555 (Exoenzymes regulatory protein AepA OS=Pectobacterium carotovorum subsp. carotovorum OX=555 GN=aepA PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 7.2e-11
Identity = 86/371 (23.18%), Postives = 149/371 (40.16%), Query Frame = 0

Query: 58  SDAAADLVVKNTLIFTSDGSLPFADSMAVLNRRILRIGTYSALQDLVGQGTKELNLGGKI 117
           S A A  V +N  I+T +   P A  + V   +I+ +G     +      T+ ++L GK 
Sbjct: 17  SHAFAHTVYENARIYTVNDRQPTASVLVVDQGKIVYVGGNDGAKPFKATATELVDLEGKT 76

Query: 118 VVPGFIDSHGHLIYQGLQMKQ--VNLHGINNKSELVRRIGEAARNTKKGNWVLGGGWN-- 177
           V+PGFI+SH H     +      V + G    S+++ ++        K N++L  G+N  
Sbjct: 77  VLPGFIESHAHPATVAVMEAGDFVYVDGARTLSQILSQLKAYLVAHPKANYLLAQGFNVA 136

Query: 178 -------------------SDLPVKL----MVMSLANNVTLKLAGVTNLTENPEGGT--V 237
                                +P+ +    M    AN+  L +A V   T +P  G    
Sbjct: 137 SLGLPQGALPTAADLDTVSESVPIVVYDSGMHAGWANSAALNVAHVDANTPDPIPGKHYF 196

Query: 238 VKTTDGDPTGLLVDSARKLVLPFIPEVTVEERREALESVSNLALSRGVTTIVDFGRYYPA 297
            +   G+PTG + +SA   V+       VE   E L+ +     S G T I D G  +  
Sbjct: 197 ERDNKGNPTGFMHESAMHNVVDAQQFNAVENVAEKLQPILKTYHSLGFTAITDVGDTFST 256

Query: 298 ESAKLSWEDFSDVYQWADSSGKMKI--RVCLFFPM-----ETWSSLHDLNQKMGQVVSPW 357
             A ++           +  GK+K+  +   F+       +  +SL  L +K  Q     
Sbjct: 257 TVAAIA---------RLNEQGKLKVYYQRGYFYDAAKSTEQNIASLKGLREKYHQ---GN 316

Query: 358 IYLGGVKGFADGSLGSHTALFHEP---DNCGIQMTERENLFNMSMESDKSKLQVAIHAIG 390
           + +   K F DG++   +   ++P    N       ++ + +    + K+   V +HAIG
Sbjct: 317 LSINLYKLFMDGTIEMDSGAMYQPYPNGNVVEPFLSQKQINDNVAAALKAGFSVHVHAIG 375

BLAST of Sed0002543 vs. ExPASy TrEMBL
Match: A0A6J1G1F2 (uncharacterized protein LOC111449791 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449791 PE=4 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 8.8e-238
Identity = 426/549 (77.60%), Postives = 453/549 (82.51%), Query Frame = 0

Query: 57  NSDAAADLVVKNTLIFTSDGSLPFADSMAVLNRRILRIGTYSALQDLVGQGTKELNLGGK 116
           +S A ADLVVKN LIFTSD SLPFADSMA+ NRRILR+GTYSA+QDLVGQGTKELNLGGK
Sbjct: 57  SSCAVADLVVKNALIFTSDDSLPFADSMAIRNRRILRVGTYSAVQDLVGQGTKELNLGGK 116

Query: 117 IVVPGFIDSHGHLIYQGLQMKQVNLHGINNKSELVRRIGEAARNTKKGNWVLGGGWNSD- 176
           IVVPGFIDSHGHLIYQGLQMKQVNLHG+N+KSE VRRI EAA+N KKGNWVLGGGWN+D 
Sbjct: 117 IVVPGFIDSHGHLIYQGLQMKQVNLHGVNHKSEFVRRIAEAAKNAKKGNWVLGGGWNNDL 176

Query: 177 ----LPVKLMV-----------------MSLANNVTLKLAGVTNLTENPEGGTVVKTTDG 236
               LP+   +                 MSLANNVTLKLAG+TNLTE+PEGGTVVKT  G
Sbjct: 177 WGGELPMASWIDDVTPSNPVLVSRIDGHMSLANNVTLKLAGITNLTEDPEGGTVVKTIGG 236

Query: 237 DPTGLLVDSARKLVLPFIPEVTVEERREALESVSNLALSRGVTTIVDFGRYYPAESAKLS 296
           DPTG+L+DSARKLVLPFIPEVTVEERREAL  VS LAL+RGVTTIVDFGRYYP ES KLS
Sbjct: 237 DPTGVLIDSARKLVLPFIPEVTVEERREALIRVSKLALARGVTTIVDFGRYYPGESVKLS 296

Query: 297 WEDFSDVYQWADSSGKMKIRVCLFFPMETWSSLHDLNQKMGQVVSPWIYLGGVKGFADGS 356
           WEDFSDVYQWADSSGKM IRVCLFFPMETWS LHDL  KMGQVVSPW+YLGGVKGFADGS
Sbjct: 297 WEDFSDVYQWADSSGKMMIRVCLFFPMETWSYLHDLIHKMGQVVSPWVYLGGVKGFADGS 356

Query: 357 LGSHTALFH-----EPDNCGIQMTERENLFNMSMESDKSKLQVAIHAIGDKANDMIVDIY 416
           LGSHTALFH     EPDNCG+QMTERENLFNM+MESDKSKLQVAIHAIGDKANDMI+DIY
Sbjct: 357 LGSHTALFHEPYVDEPDNCGMQMTERENLFNMTMESDKSKLQVAIHAIGDKANDMILDIY 416

Query: 417 ESIILTNGPRDRIFRVMSCSTLAPGAPERFGKLGTIASAQPEHLLDDTESATNKLGPQRA 476
           ES+I TNGPRDR FRV     LAPGAPERFGKLG IASAQPEHLLDD ESATNKLG QRA
Sbjct: 417 ESVISTNGPRDRRFRVEHAQHLAPGAPERFGKLGIIASAQPEHLLDDAESATNKLGAQRA 476

Query: 477 QKESFLFRSLLTCKACLAFGSDCPIYNSFLCVDRKYQSIGWYQDRNEKEPPTWDHAWIPS 536
           QKESFLFRSLLTCKACLAFGSDCP+ N           +G  +    + PPTWDHAWIPS
Sbjct: 477 QKESFLFRSLLTCKACLAFGSDCPVAN--------INPLGGIKTALRRIPPTWDHAWIPS 536

Query: 537 ECLELDEALKAYTISVAYASFLDKDLGSLSPGKLADFVVLFYRFMGRVRKQKDPLPSEAT 579
           ECL LDEA+KAYTIS AYASFLDKDLGSLSPGKLADFV+L          +      EAT
Sbjct: 537 ECLTLDEAIKAYTISAAYASFLDKDLGSLSPGKLADFVILSTNSWDEFAAE-GSASIEAT 596

BLAST of Sed0002543 vs. ExPASy TrEMBL
Match: A0A6J1HT59 (uncharacterized protein LOC111466536 OS=Cucurbita maxima OX=3661 GN=LOC111466536 PE=4 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 1.1e-237
Identity = 427/549 (77.78%), Postives = 452/549 (82.33%), Query Frame = 0

Query: 57  NSDAAADLVVKNTLIFTSDGSLPFADSMAVLNRRILRIGTYSALQDLVGQGTKELNLGGK 116
           +S A ADLVVKN LIFTSD SLPFADSM VLNRRILR+GTYSA+QDLVGQGTKELNLGGK
Sbjct: 6   SSCAVADLVVKNALIFTSDDSLPFADSMTVLNRRILRVGTYSAVQDLVGQGTKELNLGGK 65

Query: 117 IVVPGFIDSHGHLIYQGLQMKQVNLHGINNKSELVRRIGEAARNTKKGNWVLGGGWNSD- 176
           IVVPGFIDSHGHLIYQGLQMKQVNLHG+N+KSE VRRI EAA+N KKGNWVLGGGWN+D 
Sbjct: 66  IVVPGFIDSHGHLIYQGLQMKQVNLHGVNHKSEFVRRIAEAAKNAKKGNWVLGGGWNNDL 125

Query: 177 ----LPVKLMV-----------------MSLANNVTLKLAGVTNLTENPEGGTVVKTTDG 236
               LP+   +                 MSLANNVTLKLAG+TNLTE+PEGGTVVKT  G
Sbjct: 126 WGGELPMASWIDDVTPSNPVLVSRIDGHMSLANNVTLKLAGITNLTEDPEGGTVVKTIGG 185

Query: 237 DPTGLLVDSARKLVLPFIPEVTVEERREALESVSNLALSRGVTTIVDFGRYYPAESAKLS 296
           DPTG+L+DSARKLVLPFIPEVTVEERREAL  VS LAL+RGVTTIVDFGRYYP ES KLS
Sbjct: 186 DPTGVLIDSARKLVLPFIPEVTVEERREALIRVSKLALARGVTTIVDFGRYYPGESVKLS 245

Query: 297 WEDFSDVYQWADSSGKMKIRVCLFFPMETWSSLHDLNQKMGQVVSPWIYLGGVKGFADGS 356
           WEDFSDVYQWADSSGKM IRVCLFFPMETWS LHDL  KMGQVVSPW+YLGGVKGFADGS
Sbjct: 246 WEDFSDVYQWADSSGKMMIRVCLFFPMETWSYLHDLIHKMGQVVSPWVYLGGVKGFADGS 305

Query: 357 LGSHTALFH-----EPDNCGIQMTERENLFNMSMESDKSKLQVAIHAIGDKANDMIVDIY 416
           LGSHTALFH     EPDNCG+QMTERENLFNM+MESDKSKLQVAIHAIGDKANDMI+DIY
Sbjct: 306 LGSHTALFHEPYVDEPDNCGMQMTERENLFNMTMESDKSKLQVAIHAIGDKANDMILDIY 365

Query: 417 ESIILTNGPRDRIFRVMSCSTLAPGAPERFGKLGTIASAQPEHLLDDTESATNKLGPQRA 476
           ES+I TNGPRDR FRV     LAPGAPERFGKLG IASAQPEHLLDD ESATNKLG QRA
Sbjct: 366 ESVISTNGPRDRRFRVEHAQHLAPGAPERFGKLGIIASAQPEHLLDDAESATNKLGAQRA 425

Query: 477 QKESFLFRSLLTCKACLAFGSDCPIYNSFLCVDRKYQSIGWYQDRNEKEPPTWDHAWIPS 536
           QKESFLFRSLLTCKACLAFGSDCPI N           +G  +    + PPTWDHAWIPS
Sbjct: 426 QKESFLFRSLLTCKACLAFGSDCPIAN--------INPLGGIKTALRRIPPTWDHAWIPS 485

Query: 537 ECLELDEALKAYTISVAYASFLDKDLGSLSPGKLADFVVLFYRFMGRVRKQKDPLPSEAT 579
           ECL LDEA+KAYTIS AYASFLDKDLGSLSPGKLADFV+L          +      EAT
Sbjct: 486 ECLTLDEAIKAYTISAAYASFLDKDLGSLSPGKLADFVILSTNSWDEFAAE-GSASIEAT 545

BLAST of Sed0002543 vs. ExPASy TrEMBL
Match: A0A6J1CCG3 (uncharacterized protein LOC111010225 OS=Momordica charantia OX=3673 GN=LOC111010225 PE=4 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 3.2e-232
Identity = 422/549 (76.87%), Postives = 448/549 (81.60%), Query Frame = 0

Query: 57  NSDAAADLVVKNTLIFTSDGSLPFADSMAVLNRRILRIGTYSALQDLVGQGTKELNLGGK 116
           +S   ADLVVKN LIFTSD SLPFADSMA+LNRRILR+G+YSALQDLVGQGTKELNLGGK
Sbjct: 4   SSCLVADLVVKNALIFTSDDSLPFADSMAILNRRILRVGSYSALQDLVGQGTKELNLGGK 63

Query: 117 IVVPGFIDSHGHLIYQGLQMKQVNLHGINNKSELVRRIGEAARNTKKGNWVLGGGWNSDL 176
           IVVPGFIDSHGHLIYQGLQMKQVNLHG+N K+E VRRI EAARNT+KGNWVLGGGWN+DL
Sbjct: 64  IVVPGFIDSHGHLIYQGLQMKQVNLHGVNKKNEFVRRIAEAARNTEKGNWVLGGGWNNDL 123

Query: 177 ------------------PVKLM----VMSLANNVTLKLAGVTNLTENPEGGTVVKTTDG 236
                             PV L      MSLANNVTLKLAG+TNLTE+PEGGT+VKTT G
Sbjct: 124 WGGEWPMASWIDDVTASNPVLLSRIDGHMSLANNVTLKLAGITNLTEDPEGGTIVKTTGG 183

Query: 237 DPTGLLVDSARKLVLPFIPEVTVEERREALESVSNLALSRGVTTIVDFGRYYPAESAKLS 296
           DPTGLL+DSARKLVLPFIPEV+V+ERREAL  VS+LAL+RGVTTIVDFGRYYP ES KLS
Sbjct: 184 DPTGLLIDSARKLVLPFIPEVSVDERREALLRVSSLALARGVTTIVDFGRYYPGESVKLS 243

Query: 297 WEDFSDVYQWADSSGKMKIRVCLFFPMETWSSLHDLNQKMGQVVSPWIYLGGVKGFADGS 356
           WEDFSDVYQWADSS KM IRVCLFFPMETWSSLHDL  KMG VVSPWIYLGGVKGFADGS
Sbjct: 244 WEDFSDVYQWADSSEKMMIRVCLFFPMETWSSLHDLIHKMGHVVSPWIYLGGVKGFADGS 303

Query: 357 LGSHTALFH-----EPDNCGIQMTERENLFNMSMESDKSKLQVAIHAIGDKANDMIVDIY 416
           LGS TALFH     EPDN G+QMTERENLFNM+M SDKSKLQVAIHAIGDKANDM++DIY
Sbjct: 304 LGSRTALFHEPYVDEPDNYGMQMTERENLFNMTMASDKSKLQVAIHAIGDKANDMVLDIY 363

Query: 417 ESIILTNGPRDRIFRVMSCSTLAPGAPERFGKLGTIASAQPEHLLDDTESATNKLGPQRA 476
           ES+I TNGPRDR FRV     LA G PERFGKLG IASAQPEHLLDD ESATNKLG QRA
Sbjct: 364 ESVISTNGPRDRRFRVEHAQHLAHGTPERFGKLGIIASAQPEHLLDDAESATNKLGTQRA 423

Query: 477 QKESFLFRSLLTCKACLAFGSDCPIYNSFLCVDRKYQSIGWYQDRNEKEPPTWDHAWIPS 536
           QKESFLFRSLLTCKACLAFGSDCPI N           +G  +    + PPTWD AWIPS
Sbjct: 424 QKESFLFRSLLTCKACLAFGSDCPIAN--------INPMGGIKTAIRRTPPTWDRAWIPS 483

Query: 537 ECLELDEALKAYTISVAYASFLDKDLGSLSPGKLADFVVLFYRFMGRVRKQKDPLPSEAT 579
           ECL LDEALKAYTIS AYASFLDKDLGSLSPGKLADFV+L          Q      EAT
Sbjct: 484 ECLRLDEALKAYTISAAYASFLDKDLGSLSPGKLADFVILSTDSWDDFEAQ-GSASIEAT 543

BLAST of Sed0002543 vs. ExPASy TrEMBL
Match: A0A1S3BGA6 (putative amidohydrolase YtcJ isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489516 PE=4 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 5.7e-229
Identity = 416/582 (71.48%), Postives = 461/582 (79.21%), Query Frame = 0

Query: 26  INFTK--TKPPLIFLIVIPILHQSFTFEQESRLNSDAAADLVVKNTLIFTSDGSLPFADS 85
           ++FT   + PP +F I   I H           +S A ADLV+KN LIFTSD SLPFADS
Sbjct: 26  LHFTSLHSTPPSLFPIPFSI-HLQLGAPIAMLSSSCAVADLVLKNALIFTSDDSLPFADS 85

Query: 86  MAVLNRRILRIGTYSALQDLVGQGTKELNLGGKIVVPGFIDSHGHLIYQGLQMKQVNLHG 145
           MA+L+ RILR+GTYSA+QDLVG GTKELNLGGK+VVPGFID+HGHLIYQGLQMKQVNLHG
Sbjct: 86  MAILDGRILRLGTYSAVQDLVGSGTKELNLGGKVVVPGFIDAHGHLIYQGLQMKQVNLHG 145

Query: 146 INNKSELVRRIGEAARNTKKGNWVLGGGWNSDL------------------PVKLM---- 205
           +N+K E V RI EAA+NTKKGNWVLGGGWN+DL                  PV L     
Sbjct: 146 VNHKHEFVTRIAEAAKNTKKGNWVLGGGWNNDLWGGDLPMASWIDDVTPSNPVLLSRIDG 205

Query: 206 VMSLANNVTLKLAGVTNLTENPEGGTVVKTTDGDPTGLLVDSARKLVLPFIPEVTVEERR 265
            MSLANNVTLKLAG++NLTE+PEGGT+VKTT GDPTGLL+DSARKLVLPFIP+V+VEERR
Sbjct: 206 HMSLANNVTLKLAGISNLTEDPEGGTIVKTTGGDPTGLLIDSARKLVLPFIPKVSVEERR 265

Query: 266 EALESVSNLALSRGVTTIVDFGRYYPAESAKLSWEDFSDVYQWADSSGKMKIRVCLFFPM 325
           EAL   SNLAL+RGVTTIVDFGRYYP ES +LSWEDFSDVYQWADSSGKM IRVCLFFPM
Sbjct: 266 EALLRASNLALARGVTTIVDFGRYYPGESVELSWEDFSDVYQWADSSGKMMIRVCLFFPM 325

Query: 326 ETWSSLHDLNQKMGQVVSPWIYLGGVKGFADGSLGSHTALFH-----EPDNCGIQMTERE 385
           ETWSSLHDL  KMGQV+SPWIYLGGVKGFADGSLGSHTALFH     EPDNCG+Q+TERE
Sbjct: 326 ETWSSLHDLIHKMGQVLSPWIYLGGVKGFADGSLGSHTALFHEPYVDEPDNCGMQITERE 385

Query: 386 NLFNMSMESDKSKLQVAIHAIGDKANDMIVDIYESIILTNGPRDRIFRVMSCSTLAPGAP 445
            LFN++MESDKSKLQVAIHAIGDKANDM++DIYES+I TNGPRDR FRV     LAPGAP
Sbjct: 386 KLFNLTMESDKSKLQVAIHAIGDKANDMVLDIYESVISTNGPRDRRFRVEHAQHLAPGAP 445

Query: 446 ERFGKLGTIASAQPEHLLDDTESATNKLGPQRAQKESFLFRSLLTCKACLAFGSDCPIYN 505
           +RFGKLG IASAQPEHLLDD ESA NKLG  RA+KESFLFRSLLTCKA LAFGSDCP+ N
Sbjct: 446 QRFGKLGIIASAQPEHLLDDAESAANKLGAHRAEKESFLFRSLLTCKASLAFGSDCPVAN 505

Query: 506 SFLCVDRKYQSIGWYQDRNEKEPPTWDHAWIPSECLELDEALKAYTISVAYASFLDKDLG 565
                      +G  +    + PP+WDHAW+PSECL LDEA+KAYTIS AYASFLDKDLG
Sbjct: 506 --------INPLGGIRTAMRRIPPSWDHAWMPSECLALDEAIKAYTISAAYASFLDKDLG 565

Query: 566 SLSPGKLADFVVLFYRFMGRVRKQKDPLPSEATYTGGEQAYP 579
           SLSPGKLADFV+L         + +     EATYTGG QAYP
Sbjct: 566 SLSPGKLADFVILSTDSWDEF-EAEGSASIEATYTGGIQAYP 597

BLAST of Sed0002543 vs. ExPASy TrEMBL
Match: A0A5A7SUX5 (Putative amidohydrolase YtcJ isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007840 PE=4 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 1.7e-228
Identity = 408/549 (74.32%), Postives = 449/549 (81.79%), Query Frame = 0

Query: 57  NSDAAADLVVKNTLIFTSDGSLPFADSMAVLNRRILRIGTYSALQDLVGQGTKELNLGGK 116
           +S A ADLV+KN LIFTSD SLPFADSMA+L+ RILR+GTYSA+QDLVG GTKELNLGGK
Sbjct: 4   SSCAVADLVLKNALIFTSDDSLPFADSMAILDGRILRLGTYSAVQDLVGSGTKELNLGGK 63

Query: 117 IVVPGFIDSHGHLIYQGLQMKQVNLHGINNKSELVRRIGEAARNTKKGNWVLGGGWNSDL 176
           +VVPGFID+HGHLIYQGLQMKQVNLHG+N+K E V RI EAA+NTKKGNWVLGGGWN+DL
Sbjct: 64  VVVPGFIDAHGHLIYQGLQMKQVNLHGVNHKHEFVTRIAEAAKNTKKGNWVLGGGWNNDL 123

Query: 177 ------------------PVKLM----VMSLANNVTLKLAGVTNLTENPEGGTVVKTTDG 236
                             PV L      MSLANNVTLKLAG++NLTE+PEGGT+VKTT G
Sbjct: 124 WGGDLPMASWIDDVTPSNPVLLSRIDGHMSLANNVTLKLAGISNLTEDPEGGTIVKTTGG 183

Query: 237 DPTGLLVDSARKLVLPFIPEVTVEERREALESVSNLALSRGVTTIVDFGRYYPAESAKLS 296
           DPTGLL+DSARKLVLPFIP+V+VEERREAL   SNLAL+RGVTTIVDFGRYYP ES +LS
Sbjct: 184 DPTGLLIDSARKLVLPFIPKVSVEERREALLRASNLALARGVTTIVDFGRYYPGESVELS 243

Query: 297 WEDFSDVYQWADSSGKMKIRVCLFFPMETWSSLHDLNQKMGQVVSPWIYLGGVKGFADGS 356
           WEDFSDVYQWADSSGKM IRVCLFFPMETWSSLHDL  KMGQV+SPWIYLGGVKGFADGS
Sbjct: 244 WEDFSDVYQWADSSGKMMIRVCLFFPMETWSSLHDLIHKMGQVLSPWIYLGGVKGFADGS 303

Query: 357 LGSHTALFH-----EPDNCGIQMTERENLFNMSMESDKSKLQVAIHAIGDKANDMIVDIY 416
           LGSHTALFH     EPDNCG+Q+TERE LFN++MESDKSKLQVAIHAIGDKANDM++DIY
Sbjct: 304 LGSHTALFHEPYVDEPDNCGMQITEREKLFNLTMESDKSKLQVAIHAIGDKANDMVLDIY 363

Query: 417 ESIILTNGPRDRIFRVMSCSTLAPGAPERFGKLGTIASAQPEHLLDDTESATNKLGPQRA 476
           ES+I TNGPRDR FRV     LAPGAP+RFGKLG IASAQPEHLLDD ESA NKLG  RA
Sbjct: 364 ESVISTNGPRDRRFRVEHAQHLAPGAPQRFGKLGIIASAQPEHLLDDAESAANKLGAHRA 423

Query: 477 QKESFLFRSLLTCKACLAFGSDCPIYNSFLCVDRKYQSIGWYQDRNEKEPPTWDHAWIPS 536
           +KESFLFRSLLTCKA LAFGSDCP+ N           +G  +    + PP+WDHAW+PS
Sbjct: 424 EKESFLFRSLLTCKASLAFGSDCPVAN--------INPLGGIRTAMRRIPPSWDHAWMPS 483

Query: 537 ECLELDEALKAYTISVAYASFLDKDLGSLSPGKLADFVVLFYRFMGRVRKQKDPLPSEAT 579
           ECL LDEA+KAYTIS AYASFLDKDLGSLSPGKLADFV+L         + +     EAT
Sbjct: 484 ECLALDEAIKAYTISAAYASFLDKDLGSLSPGKLADFVILSTDSWDEF-EAEGSASIEAT 543

BLAST of Sed0002543 vs. TAIR 10
Match: AT3G55850.1 (Amidohydrolase family )

HSP 1 Score: 552.4 bits (1422), Expect = 4.4e-157
Identity = 291/572 (50.87%), Postives = 382/572 (66.78%), Query Frame = 0

Query: 35  LIFLIVIPILHQSFTFEQESRL-NSDAAADLVVKNTLIFTSDGSLPFADSMAVLNRRILR 94
           LI +  +P+L+  +    +S    +   ADL+V N  IFTSD SLPFADSMA+ N RIL+
Sbjct: 14  LISVAYLPLLNDLYWSTLKSLTPPAGIVADLLVTNGTIFTSDSSLPFADSMAIRNGRILK 73

Query: 95  IGTYSALQDLVGQGTKELNLGGKIVVPGFIDSHGHLIYQGLQMKQVNLHGINNKSELVRR 154
           +G+++ L+  +G GT E+NL GKIVVPG IDSH HLI  GLQM QV L G++ K E  + 
Sbjct: 74  VGSFATLKGFIGDGTMEVNLEGKIVVPGLIDSHVHLISGGLQMAQVGLRGVSQKDEFCKM 133

Query: 155 IGEAARNTKKGNWVLGGGWNSDL------------------PVKLM----VMSLANNVTL 214
           + +A +N K+G+W+LGGGWN+D                   PV L+     M+LAN++ L
Sbjct: 134 VKDAVQNAKEGSWILGGGWNNDFWGGELPSASWIDEISPRNPVWLIRMDGHMALANSLAL 193

Query: 215 KLAGVTNLTENPEGGTVVKTTDGDPTGLLVDSARKLVLPFIPEVTVEERREALESVSNLA 274
           K+AGV +LTE+P GGT+++   G+PTGLL+D+A +LV P++ E++V+ERREAL   S  A
Sbjct: 194 KIAGVISLTEDPVGGTIMRMPSGEPTGLLIDAAMELVTPWVKEISVDERREALFRASKYA 253

Query: 275 LSRGVTTIVDFGRYYPAESAKLSWEDFSDVYQWADSSGKMKIRVCLFFPMETWSSLHDLN 334
           L+RGVTT++D GRY+P  + +LSW+DF DVY +ADSS KM IR CLFFP+ TWS L DL 
Sbjct: 254 LTRGVTTVIDLGRYFPGTTDELSWKDFQDVYLYADSSKKMMIRTCLFFPITTWSRLLDLK 313

Query: 335 QKMGQVVSPWIYLGGVKGFADGSLGSHTALFHE-----PDNCGIQMTERENLFNMSMESD 394
            + G V+S W+YLGGVK F DGSLGS++ALF+E     P+N G+++ + E L N +M +D
Sbjct: 314 LQKGSVLSEWLYLGGVKAFIDGSLGSNSALFYEEYIDTPNNYGLEVMDPEKLSNFTMAAD 373

Query: 395 KSKLQVAIHAIGDKANDMIVDIYESIILTNGPRDRIFRVMSCSTLAPGAPERFGKLGTIA 454
           KS LQVAIHAIGDKANDMI+D+YES+   NG RDR FR+     LAPG+  RFG+L  +A
Sbjct: 374 KSGLQVAIHAIGDKANDMILDMYESVAAANGDRDRRFRIEHAQHLAPGSANRFGQLHIVA 433

Query: 455 SAQPEHLLDDTESATNKLGPQRAQKESFLFRSLLTCKACLAFGSDCPIYNSFLCVDRKYQ 514
           S QP+HLLDD +S   KLG +RA KES+LF+SLL   A LA GSD P+ +          
Sbjct: 434 SVQPDHLLDDADSVAKKLGSERAVKESYLFQSLLNGNALLALGSDWPVAD--------IN 493

Query: 515 SIGWYQDRNEKEPPTWDHAWIPSECLELDEALKAYTISVAYASFLDKDLGSLSPGKLADF 574
            +   +   ++ PP WDHAWIPSE +   +AL A TIS A A+FLD  LGSLSPGKLADF
Sbjct: 494 PLHSIRTAVKRIPPKWDHAWIPSERISFTDALIAQTISAARAAFLDHHLGSLSPGKLADF 553

Query: 575 VVLFYRFMGRVRKQKDPLPSEATYTGGEQAYP 579
           V+L         K        ATY GG+Q YP
Sbjct: 554 VILSTNSWDEFSKDVS-ASVLATYVGGKQLYP 576

BLAST of Sed0002543 vs. TAIR 10
Match: AT3G55850.2 (Amidohydrolase family )

HSP 1 Score: 552.4 bits (1422), Expect = 4.4e-157
Identity = 291/572 (50.87%), Postives = 382/572 (66.78%), Query Frame = 0

Query: 35  LIFLIVIPILHQSFTFEQESRL-NSDAAADLVVKNTLIFTSDGSLPFADSMAVLNRRILR 94
           LI +  +P+L+  +    +S    +   ADL+V N  IFTSD SLPFADSMA+ N RIL+
Sbjct: 21  LISVAYLPLLNDLYWSTLKSLTPPAGIVADLLVTNGTIFTSDSSLPFADSMAIRNGRILK 80

Query: 95  IGTYSALQDLVGQGTKELNLGGKIVVPGFIDSHGHLIYQGLQMKQVNLHGINNKSELVRR 154
           +G+++ L+  +G GT E+NL GKIVVPG IDSH HLI  GLQM QV L G++ K E  + 
Sbjct: 81  VGSFATLKGFIGDGTMEVNLEGKIVVPGLIDSHVHLISGGLQMAQVGLRGVSQKDEFCKM 140

Query: 155 IGEAARNTKKGNWVLGGGWNSDL------------------PVKLM----VMSLANNVTL 214
           + +A +N K+G+W+LGGGWN+D                   PV L+     M+LAN++ L
Sbjct: 141 VKDAVQNAKEGSWILGGGWNNDFWGGELPSASWIDEISPRNPVWLIRMDGHMALANSLAL 200

Query: 215 KLAGVTNLTENPEGGTVVKTTDGDPTGLLVDSARKLVLPFIPEVTVEERREALESVSNLA 274
           K+AGV +LTE+P GGT+++   G+PTGLL+D+A +LV P++ E++V+ERREAL   S  A
Sbjct: 201 KIAGVISLTEDPVGGTIMRMPSGEPTGLLIDAAMELVTPWVKEISVDERREALFRASKYA 260

Query: 275 LSRGVTTIVDFGRYYPAESAKLSWEDFSDVYQWADSSGKMKIRVCLFFPMETWSSLHDLN 334
           L+RGVTT++D GRY+P  + +LSW+DF DVY +ADSS KM IR CLFFP+ TWS L DL 
Sbjct: 261 LTRGVTTVIDLGRYFPGTTDELSWKDFQDVYLYADSSKKMMIRTCLFFPITTWSRLLDLK 320

Query: 335 QKMGQVVSPWIYLGGVKGFADGSLGSHTALFHE-----PDNCGIQMTERENLFNMSMESD 394
            + G V+S W+YLGGVK F DGSLGS++ALF+E     P+N G+++ + E L N +M +D
Sbjct: 321 LQKGSVLSEWLYLGGVKAFIDGSLGSNSALFYEEYIDTPNNYGLEVMDPEKLSNFTMAAD 380

Query: 395 KSKLQVAIHAIGDKANDMIVDIYESIILTNGPRDRIFRVMSCSTLAPGAPERFGKLGTIA 454
           KS LQVAIHAIGDKANDMI+D+YES+   NG RDR FR+     LAPG+  RFG+L  +A
Sbjct: 381 KSGLQVAIHAIGDKANDMILDMYESVAAANGDRDRRFRIEHAQHLAPGSANRFGQLHIVA 440

Query: 455 SAQPEHLLDDTESATNKLGPQRAQKESFLFRSLLTCKACLAFGSDCPIYNSFLCVDRKYQ 514
           S QP+HLLDD +S   KLG +RA KES+LF+SLL   A LA GSD P+ +          
Sbjct: 441 SVQPDHLLDDADSVAKKLGSERAVKESYLFQSLLNGNALLALGSDWPVAD--------IN 500

Query: 515 SIGWYQDRNEKEPPTWDHAWIPSECLELDEALKAYTISVAYASFLDKDLGSLSPGKLADF 574
            +   +   ++ PP WDHAWIPSE +   +AL A TIS A A+FLD  LGSLSPGKLADF
Sbjct: 501 PLHSIRTAVKRIPPKWDHAWIPSERISFTDALIAQTISAARAAFLDHHLGSLSPGKLADF 560

Query: 575 VVLFYRFMGRVRKQKDPLPSEATYTGGEQAYP 579
           V+L         K        ATY GG+Q YP
Sbjct: 561 VILSTNSWDEFSKDVS-ASVLATYVGGKQLYP 583