Sed0002244 (gene) Chayote v1

BLAST of Sed0002244 vs. NCBI nr
Match: XP_022134939.1 (dicer-like protein 4 isoform X1 [Momordica charantia])

HSP 1 Score: 2687.1 bits (6964), Expect = 0.0e+00
Identity = 1369/1640 (83.48%), Postives = 1471/1640 (89.70%), Query Frame = 0

Query: 68   DNSKPSSLA-VEKLMGLSLVGDPGAGSSSRASEKDPRRVARKYQLELCKKALEENIIVYL 127
            DN + +SL  VE  +GLSLV D   G SSRASEKDPRR ARKYQLELCKKALEENII+YL
Sbjct: 15   DNFQHTSLGPVESFVGLSLVDDQEPGPSSRASEKDPRRKARKYQLELCKKALEENIILYL 74

Query: 128  GTGCGKTHIAILLIYEMRNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVYCGG 187
            GTGCGKTHIA+LL+YEMR+LIRRSQKGICVFLAPTVALVQQQAKVIEDS DFKVRVYCGG
Sbjct: 75   GTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIEDSTDFKVRVYCGG 134

Query: 188  FKGLNSHYDWEREMEEHEVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSDHSY 247
             K LNSHYDWEREMEEHEVFVMTP+ LLR+L+HCYIKMEFVELLIFDECHHAQVKSDHSY
Sbjct: 135  SKCLNSHYDWEREMEEHEVFVMTPEILLRTLHHCYIKMEFVELLIFDECHHAQVKSDHSY 194

Query: 248  AQIMKVFYKGSVAKRPRIFGMTASPVVGKGTCNQQSLSKSINSLEKLLDAKVYSVEDRED 307
            A+IM+VFYK +VAKRPRIFGMTASPVVGKG CNQQ+LSKSINSLEKLLDAKVYSVEDRE+
Sbjct: 195  AEIMRVFYKTNVAKRPRIFGMTASPVVGKGACNQQNLSKSINSLEKLLDAKVYSVEDREE 254

Query: 308  LEAFVSSPLVKIYYYGPVANGSSSSFMTYCCQLEEVKRQCVVALGQMKGEHEGLLATKKL 367
            L+AFVSSPLVKIYYYGPVANG S SFMTYC  LE++KRQC+V LGQMKGEHE L ATKKL
Sbjct: 255  LDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGEHEDLRATKKL 314

Query: 368  LLRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNDSLSDRYLNQAA 427
            L RMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNN SLSDRYLNQAA
Sbjct: 315  LCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHSLSDRYLNQAA 374

Query: 428  EILASGCKIDGGMPDMLNVDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIVIAR 487
            EILA GCKI GGM D LN DILE PFFSKKLL+LI ILSSF HRLNMKCIIFVNRIVIAR
Sbjct: 375  EILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIAR 434

Query: 488  SLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGEEGL 547
            SLSYILQNL+FLA+WKCD+LVGVHSKLRSMSRKTMN IL KFRSGELNLL+ATKVGEEGL
Sbjct: 435  SLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGL 494

Query: 548  DIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDENHMN 607
            DIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAF+VD GNEKE GLID FQKDEN M+
Sbjct: 495  DIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMD 554

Query: 608  REISSRSSNETFQSHEESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKFFYY 667
            REISSRSS+E F SHEESIYRV STGAS+TSG SISLLHE+CSKLPHDEYFDPKPKFFYY
Sbjct: 555  REISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYY 614

Query: 668  DDLEGTICHAILPSNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPMRDN 727
            DDL GT+CHAILPSNAPI Q++SR Q SR+ AKKDAC+KAIEELHKLGA NDFLLPMRD+
Sbjct: 615  DDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLPMRDD 674

Query: 728  AIEEELELASCDSDSSEDETSRRELHEMLVPTALKDSWTGSGNVILNCYQIKISPHPGDR 787
            A EEELEL S DSD SEDETSR ELHEMLVP ALKDSWTGSG  ILNCYQIK SPHP DR
Sbjct: 675  ANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQIKFSPHPRDR 734

Query: 788  IYKEFGLFVKAPLPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQEMFL 847
            IYKEFGLFVKA LPQEAER+D+ELHLAR R V VNLVPSGVVELLEDEITQAESFQEMFL
Sbjct: 735  IYKEFGLFVKAALPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFL 794

Query: 848  KVILDRSEFVQDYVPLRNNVSGSISSASYLLLPMIFQDNEGSSLIDWKVIRRCLSSQIFQ 907
            KVILDRSEFVQ+++PLRNNVS SISS SYLLLP+IF DNE S  IDWKVIRRCL S IFQ
Sbjct: 795  KVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVIRRCLFSPIFQ 854

Query: 908  NHACSAVRETASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSR--Y 967
                S V+ETA S  HLTLYDGRRR  DI+NSLVYVPYKREFFFVT IV+ KNGYSR  Y
Sbjct: 855  IPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKGKNGYSRSHY 914

Query: 968  KNSGSLSHFEHLLERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLIDL 1027
            KNS S SHFEHL+ERF IHLKYPEQPLL AKPLFHLHNLLHDRR  +SEAC L E+ IDL
Sbjct: 915  KNSSSSSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEACYLQEYFIDL 974

Query: 1028 PPELCQLKITGFTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRILE 1087
            PPELCQLKI GF+KDIGSSISLLPS MH LEN LVAIELKC LAAAFP  +EVT+NRILE
Sbjct: 975  PPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGAEVTANRILE 1034

Query: 1088 ALTTEKCQERISLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFKL 1147
            ALTTEKCQERISLERLEILGD FLKFAVAR+LFLTHDK DEGELTRRRSYLVKNINLFKL
Sbjct: 1035 ALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYLVKNINLFKL 1094

Query: 1148 ATRKNLQVYIRDQPFEPNQFYSLGRPCPRICNEETRKDIHSH----KNAKGGETKCSKGH 1207
            ATRKNLQVYIRDQPFEP+QFY+LGRPCPRICNEETR+DIHSH    KNA+  ETKCS+GH
Sbjct: 1095 ATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNAQASETKCSRGH 1154

Query: 1208 HWLHKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRLA 1267
            HWLHKKTISDVVEALVGAFLVDSGFKAAI+FLKWIGIQVEFEAS VT+A M+SNAY+ LA
Sbjct: 1155 HWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALMSSNAYIILA 1214

Query: 1268 DCISISALQDSLGHQFLYKGLLLQALIHPSYHRHGGGCYQRLEFLGDAVLDYLITSYLYS 1327
            D I+IS LQ+SLGHQFL+KGLLLQAL+HPSYH+HGGGCYQRLEFLGDAVLDYLITSYLYS
Sbjct: 1215 DRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLDYLITSYLYS 1274

Query: 1328 VYPKLKPGLVTDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIKSPPSE 1387
             YPKLKPGL+TDLRSVFV NEAFAN++VDR FYK+LICDSTSLQ DIKSYVNFIK+P S 
Sbjct: 1275 AYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYVNFIKAPSSG 1334

Query: 1388 RGSLEQPTCPKVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRDI 1447
             GSL  P+CPK LGD+VES VGAVLVDTGFDMNYVWKIMLSFLD I+SFSGFQLSPIRDI
Sbjct: 1335 SGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSGFQLSPIRDI 1394

Query: 1448 TEFCQDCGWKLQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKLK 1507
             EFCQDCG  LQFN +K  KY+SV+A+VKG+NFH TASAA+RRK+DA+KIAANLIL+KLK
Sbjct: 1395 IEFCQDCGSNLQFNESKTGKYYSVEADVKGDNFHATASAAHRRKRDAKKIAANLILTKLK 1454

Query: 1508 EKGFIPEVSSLEEILRSRKKMEPELIGFDETPSVSIDQADNGSGTIPGSEHSGAELDPCV 1567
             +G IPEV+SLEEIL+S +KMEP+LIG+DETPSV++DQADNG GTI G E SGA+LDP V
Sbjct: 1455 AQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTMDQADNGFGTIKGLESSGADLDPFV 1514

Query: 1568 NSEFSNSHSVRITRLSEAPEFSSVAAEEELKPS-TFGG----TNLQSSSGRSSKTTARSR 1627
            +SE  N+H  RITR+S+    SS AA+E+L PS  F G    T++QSSSGR  K TARSR
Sbjct: 1515 HSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDGHDSPTDVQSSSGRLCKATARSR 1574

Query: 1628 LYEICAANHWNQPSFDCMNEEGPGHLKMFTYKVVLEIEEAPDTIFEFFGASHSRKKAAAE 1687
            LYEICAANHW+ PSFDCMNEEGP HLKMFTYKVVLEIEEAPDTIF+FFGA H RKKAAAE
Sbjct: 1575 LYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPDTIFKFFGAPHLRKKAAAE 1634

Query: 1688 HAAEAALWYLKKEGYWNETD 1696
            HAAEAA+WYLKKEGY  ETD
Sbjct: 1635 HAAEAAIWYLKKEGYLPETD 1653

BLAST of Sed0002244 vs. NCBI nr
Match: XP_038879795.1 (dicer-like protein 4 isoform X2 [Benincasa hispida])

HSP 1 Score: 2678.7 bits (6942), Expect = 0.0e+00
Identity = 1355/1639 (82.67%), Postives = 1462/1639 (89.20%), Query Frame = 0

Query: 65   DFIDNSKPSSLAVEKLMGLSLVGDPGAGSSSRASEKDPRRVARKYQLELCKKALEENIIV 124
            D ++      + VE+ MGLS   D  AGSS+R SEKDPRR+ARKYQLELCKKALEENIIV
Sbjct: 12   DSVNFQPTGFVPVERFMGLSQNDDQAAGSSTRTSEKDPRRIARKYQLELCKKALEENIIV 71

Query: 125  YLGTGCGKTHIAILLIYEMRNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVYC 184
            YLGTGCGKTHIAILLIYEM +LIRRSQKGICVFLAPTVALVQQQAKVIEDS DFKVRVYC
Sbjct: 72   YLGTGCGKTHIAILLIYEMSHLIRRSQKGICVFLAPTVALVQQQAKVIEDSTDFKVRVYC 131

Query: 185  GGFKGLNSHYDWEREMEEHEVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSDH 244
            GG K LN+HYDWE EMEE+EVFVMTP+ LLR+L+HCYIKM+FVELLIFDECHHAQVKSDH
Sbjct: 132  GGSKCLNTHYDWETEMEEYEVFVMTPEILLRNLHHCYIKMDFVELLIFDECHHAQVKSDH 191

Query: 245  SYAQIMKVFYKGSVAKRPRIFGMTASPVVGKGTCNQQSLSKSINSLEKLLDAKVYSVEDR 304
            SYA+IM+VFYK +  KRPRIFGMTASPVVGKG CNQQ+LS+SINSLEKLLDAKVYSVE R
Sbjct: 192  SYAEIMRVFYKANEPKRPRIFGMTASPVVGKGACNQQNLSRSINSLEKLLDAKVYSVEAR 251

Query: 305  EDLEAFVSSPLVKIYYYGPVANGSSSSFMTYCCQLEEVKRQCVVALGQMKGEHEGLLATK 364
            E+L+AFV SPLVKIYYYGPVANGSSSSFMTYCC LE++K +C++ALGQMK EHE LLATK
Sbjct: 252  EELDAFVLSPLVKIYYYGPVANGSSSSFMTYCCLLEDIKHKCILALGQMKCEHEVLLATK 311

Query: 365  KLLLRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNDSLSDRYLNQ 424
            +LL RMH NIIFCLE+LGVWGALQASKILLSGDNSE+SELIEAAEGN  NDSLSD+YLNQ
Sbjct: 312  RLLCRMHKNIIFCLESLGVWGALQASKILLSGDNSEQSELIEAAEGNLKNDSLSDKYLNQ 371

Query: 425  AAEILASGCKIDGGMPDMLNVDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIVI 484
            AAE+ ASGCKIDGGM DMLNVDILEDPFFSKKLLRLIGILSSF HRLNMKCIIFVNRIVI
Sbjct: 372  AAEVFASGCKIDGGMSDMLNVDILEDPFFSKKLLRLIGILSSFRHRLNMKCIIFVNRIVI 431

Query: 485  ARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGEE 544
            ARSLSYILQNL FL+YWKCDFLVGVHSKLRSMSRKTM+ ILTKF+SGELNLLIATKVGEE
Sbjct: 432  ARSLSYILQNLNFLSYWKCDFLVGVHSKLRSMSRKTMSHILTKFQSGELNLLIATKVGEE 491

Query: 545  GLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDENH 604
            GLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAF+VD GNEKE GLID FQKDEN 
Sbjct: 492  GLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENR 551

Query: 605  MNREISSRSSNETFQSHEESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKFF 664
            MNREISSRSSNETF SHEESIYRVVSTGAS+TSG SISLLHEYCSKLPHDEYFDPKPKFF
Sbjct: 552  MNREISSRSSNETFCSHEESIYRVVSTGASITSGRSISLLHEYCSKLPHDEYFDPKPKFF 611

Query: 665  YYDDLEGTICHAILPSNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPMR 724
            YYDDLEGT+CHAILPSNAPI Q+VS  Q SRDAAKK ACLKA+EELHKLGA ND+LLPMR
Sbjct: 612  YYDDLEGTVCHAILPSNAPITQIVSGAQSSRDAAKKVACLKAVEELHKLGALNDYLLPMR 671

Query: 725  DNAIEEELELASCDSDSSEDETSRRELHEMLVPTALKDSWTGSGNVILNCYQIKISPHPG 784
            DNA E+ELEL S DSDSS+DE SRRELHEM+ P ALK+SWTGS  ++L CY IK +PHP 
Sbjct: 672  DNADEKELELDSSDSDSSKDENSRRELHEMIFPAALKESWTGSEYLVLYCYHIKFTPHPR 731

Query: 785  DRIYKEFGLFVKAPLPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQEM 844
            DRIYKEFGLFVKAPLP+EAER+DLELHLAR R V+VNL+PSGVVELLEDEI QAESFQEM
Sbjct: 732  DRIYKEFGLFVKAPLPEEAERMDLELHLARRRSVRVNLMPSGVVELLEDEIIQAESFQEM 791

Query: 845  FLKVILDRSEFVQDYVPLRNNVSGSISSASYLLLPMIFQDNEGSSLIDWKVIRRCLSSQI 904
            FLKVILDRSEFVQ+Y+PLRNN S SIS  SYLLLP+ F DNE S +IDW+VI+RCLSSQI
Sbjct: 792  FLKVILDRSEFVQEYIPLRNNDSRSISPTSYLLLPVTFHDNEESVIIDWRVIKRCLSSQI 851

Query: 905  FQNHACSAVRETASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSRY 964
            FQNHACS V+E ASS THLTLYDGRRR  DIENSLVYVPYK EFFFVT+IV  KNGYS+Y
Sbjct: 852  FQNHACSIVKEAASSYTHLTLYDGRRRSNDIENSLVYVPYKGEFFFVTNIVWGKNGYSQY 911

Query: 965  KNSGSLSHFEHLLERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLIDL 1024
            KNSGS SHFEHL  +F IHLK PEQPLLCAKPLF LHNLLH+R+  DS+A  L E+ IDL
Sbjct: 912  KNSGSSSHFEHLRSKFGIHLKSPEQPLLCAKPLFFLHNLLHNRKQEDSDAHYLEEYFIDL 971

Query: 1025 PPELCQLKITGFTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRILE 1084
            PPELCQLKI GF+KDIGSSISLLPS MH LEN LVAIELKC LAAAFPA +EVT+NRILE
Sbjct: 972  PPELCQLKICGFSKDIGSSISLLPSIMHRLENLLVAIELKCRLAAAFPAGAEVTANRILE 1031

Query: 1085 ALTTEKCQERISLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFKL 1144
            ALTTEKCQERISLERLEILGD+FLKFAVAR+LFLTHDKFDEGELTRRRSYLVKN NL KL
Sbjct: 1032 ALTTEKCQERISLERLEILGDSFLKFAVARYLFLTHDKFDEGELTRRRSYLVKNFNLLKL 1091

Query: 1145 ATRKNLQVYIRDQPFEPNQFYSLGRPCPRICNEETRKDIHSH---KNAKGGETKCSKGHH 1204
            ATRKNLQVYIRDQPFEP+QFY LGRPCPRICNEETRKDIHSH    N +  ETKCSKGHH
Sbjct: 1092 ATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRKDIHSHDDATNTRASETKCSKGHH 1151

Query: 1205 WLHKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRLAD 1264
            WL KKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFE+S VTDA MASN Y+ LAD
Sbjct: 1152 WLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFESSLVTDALMASNNYVLLAD 1211

Query: 1265 CISISALQDSLGHQFLYKGLLLQALIHPSYHRHGGGCYQRLEFLGDAVLDYLITSYLYSV 1324
               ISA QDSLGHQFL+KGLLLQAL+HPSYH+HGGGCYQRLEFLGDAVLDYLITSYLYS 
Sbjct: 1212 RTDISAFQDSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLDYLITSYLYSA 1271

Query: 1325 YPKLKPGLVTDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIKSPPSER 1384
            YPKLKPG +TDLRSVFV NEAFAN++VDRFFYKFL+CDSTSL  DIKSYV+FIKS PSER
Sbjct: 1272 YPKLKPGQLTDLRSVFVRNEAFANVTVDRFFYKFLLCDSTSLLSDIKSYVDFIKS-PSER 1331

Query: 1385 GSLEQPTCPKVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRDIT 1444
             SLEQP CPK LGD+VES VGAVLVDTGFDMNYVWKIMLSF+D IMSFSGFQLSPIRD+ 
Sbjct: 1332 DSLEQPRCPKALGDLVESSVGAVLVDTGFDMNYVWKIMLSFVDPIMSFSGFQLSPIRDLI 1391

Query: 1445 EFCQDCGWKLQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKLKE 1504
            EFCQ+CGWKL+FN++K+E Y+SVKAEVKG NFH+TASAANRRKKDAEKIAANLIL+KLK 
Sbjct: 1392 EFCQNCGWKLEFNSSKMEGYYSVKAEVKGGNFHDTASAANRRKKDAEKIAANLILTKLKA 1451

Query: 1505 KGFIPEVSSLEEILRSRKKMEPELIGFDETPSVSIDQADNGSGTIPGSEHSGAELDPCVN 1564
            KGF+PEV+SLEEIL+S  +MEP+LIG+DETP   +DQ DNG  TI G E S A+L+P V+
Sbjct: 1452 KGFLPEVNSLEEILKSSSRMEPKLIGYDETPFGMVDQVDNGLETINGPEFSNADLNPRVH 1511

Query: 1565 SEFSNSHSVRITRLSEAPEFSSVAAEEELKPSTF-----GGTNLQSSSGRSSKTTARSRL 1624
             E  NSH V ITR+S  P  SSVAA E+LKPS          +LQSSSG S KTTARSRL
Sbjct: 1512 REVDNSHPVHITRISRTPVSSSVAAGEQLKPSMALEGHDSPADLQSSSGWSGKTTARSRL 1571

Query: 1625 YEICAANHWNQPSFDCMNEEGPGHLKMFTYKVVLEIEEAPDTIFEFFGASHSRKKAAAEH 1684
            YEICAANHWN+PSFDCMNEEGP HLKMFTYKVVLE+EEAPDTIFEF GA H RKKAAAEH
Sbjct: 1572 YEICAANHWNRPSFDCMNEEGPSHLKMFTYKVVLEMEEAPDTIFEFVGAPHLRKKAAAEH 1631

Query: 1685 AAEAALWYLKKEGYWNETD 1696
            AAEAALWYLKK GY  ETD
Sbjct: 1632 AAEAALWYLKKAGYLPETD 1649

BLAST of Sed0002244 vs. NCBI nr
Match: XP_038879794.1 (dicer-like protein 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 2676.4 bits (6936), Expect = 0.0e+00
Identity = 1356/1640 (82.68%), Postives = 1463/1640 (89.21%), Query Frame = 0

Query: 65   DFIDNSKPSSLAVEKLMGLSLVGDPGAGSSSRASEKDPRRVARKYQLELCKKALEENIIV 124
            D ++      + VE+ MGLS   D  AGSS+R SEKDPRR+ARKYQLELCKKALEENIIV
Sbjct: 12   DSVNFQPTGFVPVERFMGLSQNDDQAAGSSTRTSEKDPRRIARKYQLELCKKALEENIIV 71

Query: 125  YLGTGCGKTHIAILLIYEMRNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVYC 184
            YLGTGCGKTHIAILLIYEM +LIRRSQKGICVFLAPTVALVQQQAKVIEDS DFKVRVYC
Sbjct: 72   YLGTGCGKTHIAILLIYEMSHLIRRSQKGICVFLAPTVALVQQQAKVIEDSTDFKVRVYC 131

Query: 185  GGFKGLNSHYDWEREMEEHEVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSDH 244
            GG K LN+HYDWE EMEE+EVFVMTP+ LLR+L+HCYIKM+FVELLIFDECHHAQVKSDH
Sbjct: 132  GGSKCLNTHYDWETEMEEYEVFVMTPEILLRNLHHCYIKMDFVELLIFDECHHAQVKSDH 191

Query: 245  SYAQIMKVFYKGSVAKRPRIFGMTASPVVGKGTCNQQSLSKSINSLEKLLDAKVYSVEDR 304
            SYA+IM+VFYK +  KRPRIFGMTASPVVGKG CNQQ+LS+SINSLEKLLDAKVYSVE R
Sbjct: 192  SYAEIMRVFYKANEPKRPRIFGMTASPVVGKGACNQQNLSRSINSLEKLLDAKVYSVEAR 251

Query: 305  EDLEAFVSSPLVKIYYYGPVANGSSSSFMTYCCQLEEVK-RQCVVALGQMKGEHEGLLAT 364
            E+L+AFV SPLVKIYYYGPVANGSSSSFMTYCC LE++K +QC++ALGQMK EHE LLAT
Sbjct: 252  EELDAFVLSPLVKIYYYGPVANGSSSSFMTYCCLLEDIKHKQCILALGQMKCEHEVLLAT 311

Query: 365  KKLLLRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNDSLSDRYLN 424
            K+LL RMH NIIFCLE+LGVWGALQASKILLSGDNSE+SELIEAAEGN  NDSLSD+YLN
Sbjct: 312  KRLLCRMHKNIIFCLESLGVWGALQASKILLSGDNSEQSELIEAAEGNLKNDSLSDKYLN 371

Query: 425  QAAEILASGCKIDGGMPDMLNVDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIV 484
            QAAE+ ASGCKIDGGM DMLNVDILEDPFFSKKLLRLIGILSSF HRLNMKCIIFVNRIV
Sbjct: 372  QAAEVFASGCKIDGGMSDMLNVDILEDPFFSKKLLRLIGILSSFRHRLNMKCIIFVNRIV 431

Query: 485  IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGE 544
            IARSLSYILQNL FL+YWKCDFLVGVHSKLRSMSRKTM+ ILTKF+SGELNLLIATKVGE
Sbjct: 432  IARSLSYILQNLNFLSYWKCDFLVGVHSKLRSMSRKTMSHILTKFQSGELNLLIATKVGE 491

Query: 545  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDEN 604
            EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAF+VD GNEKE GLID FQKDEN
Sbjct: 492  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDEN 551

Query: 605  HMNREISSRSSNETFQSHEESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKF 664
             MNREISSRSSNETF SHEESIYRVVSTGAS+TSG SISLLHEYCSKLPHDEYFDPKPKF
Sbjct: 552  RMNREISSRSSNETFCSHEESIYRVVSTGASITSGRSISLLHEYCSKLPHDEYFDPKPKF 611

Query: 665  FYYDDLEGTICHAILPSNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPM 724
            FYYDDLEGT+CHAILPSNAPI Q+VS  Q SRDAAKK ACLKA+EELHKLGA ND+LLPM
Sbjct: 612  FYYDDLEGTVCHAILPSNAPITQIVSGAQSSRDAAKKVACLKAVEELHKLGALNDYLLPM 671

Query: 725  RDNAIEEELELASCDSDSSEDETSRRELHEMLVPTALKDSWTGSGNVILNCYQIKISPHP 784
            RDNA E+ELEL S DSDSS+DE SRRELHEM+ P ALK+SWTGS  ++L CY IK +PHP
Sbjct: 672  RDNADEKELELDSSDSDSSKDENSRRELHEMIFPAALKESWTGSEYLVLYCYHIKFTPHP 731

Query: 785  GDRIYKEFGLFVKAPLPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQE 844
             DRIYKEFGLFVKAPLP+EAER+DLELHLAR R V+VNL+PSGVVELLEDEI QAESFQE
Sbjct: 732  RDRIYKEFGLFVKAPLPEEAERMDLELHLARRRSVRVNLMPSGVVELLEDEIIQAESFQE 791

Query: 845  MFLKVILDRSEFVQDYVPLRNNVSGSISSASYLLLPMIFQDNEGSSLIDWKVIRRCLSSQ 904
            MFLKVILDRSEFVQ+Y+PLRNN S SIS  SYLLLP+ F DNE S +IDW+VI+RCLSSQ
Sbjct: 792  MFLKVILDRSEFVQEYIPLRNNDSRSISPTSYLLLPVTFHDNEESVIIDWRVIKRCLSSQ 851

Query: 905  IFQNHACSAVRETASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSR 964
            IFQNHACS V+E ASS THLTLYDGRRR  DIENSLVYVPYK EFFFVT+IV  KNGYS+
Sbjct: 852  IFQNHACSIVKEAASSYTHLTLYDGRRRSNDIENSLVYVPYKGEFFFVTNIVWGKNGYSQ 911

Query: 965  YKNSGSLSHFEHLLERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLID 1024
            YKNSGS SHFEHL  +F IHLK PEQPLLCAKPLF LHNLLH+R+  DS+A  L E+ ID
Sbjct: 912  YKNSGSSSHFEHLRSKFGIHLKSPEQPLLCAKPLFFLHNLLHNRKQEDSDAHYLEEYFID 971

Query: 1025 LPPELCQLKITGFTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRIL 1084
            LPPELCQLKI GF+KDIGSSISLLPS MH LEN LVAIELKC LAAAFPA +EVT+NRIL
Sbjct: 972  LPPELCQLKICGFSKDIGSSISLLPSIMHRLENLLVAIELKCRLAAAFPAGAEVTANRIL 1031

Query: 1085 EALTTEKCQERISLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFK 1144
            EALTTEKCQERISLERLEILGD+FLKFAVAR+LFLTHDKFDEGELTRRRSYLVKN NL K
Sbjct: 1032 EALTTEKCQERISLERLEILGDSFLKFAVARYLFLTHDKFDEGELTRRRSYLVKNFNLLK 1091

Query: 1145 LATRKNLQVYIRDQPFEPNQFYSLGRPCPRICNEETRKDIHSH---KNAKGGETKCSKGH 1204
            LATRKNLQVYIRDQPFEP+QFY LGRPCPRICNEETRKDIHSH    N +  ETKCSKGH
Sbjct: 1092 LATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRKDIHSHDDATNTRASETKCSKGH 1151

Query: 1205 HWLHKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRLA 1264
            HWL KKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFE+S VTDA MASN Y+ LA
Sbjct: 1152 HWLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFESSLVTDALMASNNYVLLA 1211

Query: 1265 DCISISALQDSLGHQFLYKGLLLQALIHPSYHRHGGGCYQRLEFLGDAVLDYLITSYLYS 1324
            D   ISA QDSLGHQFL+KGLLLQAL+HPSYH+HGGGCYQRLEFLGDAVLDYLITSYLYS
Sbjct: 1212 DRTDISAFQDSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLDYLITSYLYS 1271

Query: 1325 VYPKLKPGLVTDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIKSPPSE 1384
             YPKLKPG +TDLRSVFV NEAFAN++VDRFFYKFL+CDSTSL  DIKSYV+FIKS PSE
Sbjct: 1272 AYPKLKPGQLTDLRSVFVRNEAFANVTVDRFFYKFLLCDSTSLLSDIKSYVDFIKS-PSE 1331

Query: 1385 RGSLEQPTCPKVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRDI 1444
            R SLEQP CPK LGD+VES VGAVLVDTGFDMNYVWKIMLSF+D IMSFSGFQLSPIRD+
Sbjct: 1332 RDSLEQPRCPKALGDLVESSVGAVLVDTGFDMNYVWKIMLSFVDPIMSFSGFQLSPIRDL 1391

Query: 1445 TEFCQDCGWKLQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKLK 1504
             EFCQ+CGWKL+FN++K+E Y+SVKAEVKG NFH+TASAANRRKKDAEKIAANLIL+KLK
Sbjct: 1392 IEFCQNCGWKLEFNSSKMEGYYSVKAEVKGGNFHDTASAANRRKKDAEKIAANLILTKLK 1451

Query: 1505 EKGFIPEVSSLEEILRSRKKMEPELIGFDETPSVSIDQADNGSGTIPGSEHSGAELDPCV 1564
             KGF+PEV+SLEEIL+S  +MEP+LIG+DETP   +DQ DNG  TI G E S A+L+P V
Sbjct: 1452 AKGFLPEVNSLEEILKSSSRMEPKLIGYDETPFGMVDQVDNGLETINGPEFSNADLNPRV 1511

Query: 1565 NSEFSNSHSVRITRLSEAPEFSSVAAEEELKPSTF-----GGTNLQSSSGRSSKTTARSR 1624
            + E  NSH V ITR+S  P  SSVAA E+LKPS          +LQSSSG S KTTARSR
Sbjct: 1512 HREVDNSHPVHITRISRTPVSSSVAAGEQLKPSMALEGHDSPADLQSSSGWSGKTTARSR 1571

Query: 1625 LYEICAANHWNQPSFDCMNEEGPGHLKMFTYKVVLEIEEAPDTIFEFFGASHSRKKAAAE 1684
            LYEICAANHWN+PSFDCMNEEGP HLKMFTYKVVLE+EEAPDTIFEF GA H RKKAAAE
Sbjct: 1572 LYEICAANHWNRPSFDCMNEEGPSHLKMFTYKVVLEMEEAPDTIFEFVGAPHLRKKAAAE 1631

Query: 1685 HAAEAALWYLKKEGYWNETD 1696
            HAAEAALWYLKK GY  ETD
Sbjct: 1632 HAAEAALWYLKKAGYLPETD 1650

BLAST of Sed0002244 vs. NCBI nr
Match: XP_023515970.1 (dicer-like protein 4 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2657.1 bits (6886), Expect = 0.0e+00
Identity = 1360/1651 (82.37%), Postives = 1460/1651 (88.43%), Query Frame = 0

Query: 64   SDFIDNSKPSSLAVEKLMGLSLVGDPGAGSSSRASEKDPRRVARKYQLELCKKALEENII 123
            +D ++    SS +VE+ MGLS V D GAGSS R S KDPRR+ARKYQLELC+KALEENII
Sbjct: 11   ADSVNFQPTSSASVERFMGLSPVDDQGAGSSIRTSGKDPRRIARKYQLELCEKALEENII 70

Query: 124  VYLGTGCGKTHIAILLIYEMRNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVY 183
            VYLGTGCGKTHIAILLIYEM +LIRR QKGICVFLAPTVALVQQQAKVIEDSIDFKV+VY
Sbjct: 71   VYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIEDSIDFKVKVY 130

Query: 184  CGGFKGLNSHYDWEREMEEHEVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSD 243
            CGG K LNSHYDWEREMEE+EVFVMTP+ LLR+L+HCYIKMEFVELLIFDECHHAQVKSD
Sbjct: 131  CGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIKMEFVELLIFDECHHAQVKSD 190

Query: 244  HSYAQIMKVFYKGSVAKRPRIFGMTASPVVGKGTCNQQSLSKSINSLEKLLDAKVYSVED 303
            HSYA+IM+VFYK +  KRPRIFGMTASPVVGKG CNQQ+LSKSINSLEKLLDAKVYSVED
Sbjct: 191  HSYAEIMRVFYKANEHKRPRIFGMTASPVVGKGACNQQNLSKSINSLEKLLDAKVYSVED 250

Query: 304  REDLEAFVSSPLVKIYYYGPVANGSSSSFMTYCCQLEEVKRQCVVALGQMKGEHEGLLAT 363
            RE+LEAFVSSP  KIYYYGPVA GSSSSFMTYCC LE+VK QC+VALGQMKGEHEGLLAT
Sbjct: 251  REELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKCQCIVALGQMKGEHEGLLAT 310

Query: 364  KKLLLRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNDSLSDRYLN 423
            KKLL RMH NIIFCLENLGVWGALQASKILLS DNSERSELIEAAE NP +DS+SDRYLN
Sbjct: 311  KKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSELIEAAEANPKHDSISDRYLN 370

Query: 424  QAAEILASGCKIDGGMPDMLNVDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIV 483
            QAAEI ASGCKID G+ DMLNVDIL++P FSKKLLRLIGILSSF HR NMKCIIFVNRIV
Sbjct: 371  QAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGILSSFRHRPNMKCIIFVNRIV 430

Query: 484  IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGE 543
            IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMN ILTKFRSGELNLLIATKVGE
Sbjct: 431  IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHILTKFRSGELNLLIATKVGE 490

Query: 544  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDEN 603
            EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAF+VD GNEKE GLID F KDE 
Sbjct: 491  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFHKDEK 550

Query: 604  HMNREISSRSSNETFQSHEESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKF 663
             MNREISSRSS ETF  HEESIYRVVSTGASVTSG SISLLHEYCSKLPHD YFDPKPKF
Sbjct: 551  RMNREISSRSSIETFDGHEESIYRVVSTGASVTSGRSISLLHEYCSKLPHDTYFDPKPKF 610

Query: 664  FYYDDLEGTICHAILPSNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPM 723
            FY DDL GTICHAILPSNAPIPQ+VSR Q +  AAKKDACLKA+EELHKLGA +D+LLPM
Sbjct: 611  FYCDDLGGTICHAILPSNAPIPQIVSRPQPAGVAAKKDACLKAVEELHKLGALSDYLLPM 670

Query: 724  RDNAIEEELELASCDSDSSEDETSRRELHEMLVPTALKDSWTGSGNVILNCYQIKISPHP 783
            RDNA E ELEL S DSDSSEDETSRRELHEM+ P A K+SWTG G VIL CY IK +P+P
Sbjct: 671  RDNANEGELELDS-DSDSSEDETSRRELHEMISPAAFKESWTGFGYVILYCYHIKFTPNP 730

Query: 784  GDRIYKEFGLFVKAPLPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQE 843
             DRIYKEFGLFVKAPLPQEA R+DLELHLA  R VKVNL+PS VVELLE+EITQAE FQE
Sbjct: 731  EDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMPSRVVELLENEITQAECFQE 790

Query: 844  MFLKVILDRSEFVQDYVPLRNNVSGSISSASYLLLPMIFQDNEGSSLIDWKVIRRCLSSQ 903
            MFLKVILDRSEFV DY+PLRNNVS SISS SYLLLP+I  DNEGS  IDWKVIRRCLSSQ
Sbjct: 791  MFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHDNEGSVFIDWKVIRRCLSSQ 850

Query: 904  IFQNHACSAVRETASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSR 963
            IFQNHACS V+ETASSDTHL LYDGRRR  DIENSLVYVPYKREFFFVT+IVR KNGYS+
Sbjct: 851  IFQNHACSIVKETASSDTHLMLYDGRRRSSDIENSLVYVPYKREFFFVTNIVRRKNGYSQ 910

Query: 964  YKNSGSLSHFEHLLERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLID 1023
            YK+SGSLSHFEHL  RF IHLKYPEQPLLCAKPLF LHNLLH+R+  DSEA  L E+ ID
Sbjct: 911  YKDSGSLSHFEHLKTRFGIHLKYPEQPLLCAKPLFSLHNLLHNRKQKDSEAPYLEEYFID 970

Query: 1024 LPPELCQLKITGFTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRIL 1083
            + PELCQLKITGF+KDIGSSISLLPS MH LEN LVAIELKC LAAAFPA +EVT+NRIL
Sbjct: 971  IAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPAGAEVTANRIL 1030

Query: 1084 EALTTEKCQERISLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFK 1143
            EALTTEKCQERISLERLEILGD+FLKFAVAR+LFL HDKFDEGELTRRRSYLVKNINLFK
Sbjct: 1031 EALTTEKCQERISLERLEILGDSFLKFAVARYLFLAHDKFDEGELTRRRSYLVKNINLFK 1090

Query: 1144 LATRKNLQVYIRDQPFEPNQFYSLGRPCPRICNEETRKDIHSH----KNAKGGETKCSKG 1203
            LATRKNLQVYIRDQPFEP+QFY LGRPCPRICNEETR DIHSH     NAK  ETKCSKG
Sbjct: 1091 LATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRNDIHSHDNATNNAKASETKCSKG 1150

Query: 1204 HHWLHKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRL 1263
            HHWL KKTISDVVEALVGAFLVDSGFKAAIAFLKWIGI+VEFEASQVT+A +AS AY+ L
Sbjct: 1151 HHWLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIKVEFEASQVTNALVASKAYVLL 1210

Query: 1264 ADCISISALQDSLGHQFLYKGLLLQALIHPSYHRHGGGCYQRLEFLGDAVLDYLITSYLY 1323
            AD I+IS+LQ SLGH+FL+KGLLLQAL+HP+YH+HGGGCYQRLEFLGDAVLDYLI SYLY
Sbjct: 1211 ADTINISSLQSSLGHKFLHKGLLLQALVHPTYHKHGGGCYQRLEFLGDAVLDYLIMSYLY 1270

Query: 1324 SVYPKLKPGLVTDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIKSPPS 1383
            S YPKLKPGL+TDLRSVFV NEAFAN++VDRFFYKFL+CDS SLQ DIKSYV+FIK+PPS
Sbjct: 1271 SAYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLCDSASLQSDIKSYVDFIKAPPS 1330

Query: 1384 ERGSLEQPTCPKVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRD 1443
            ER SLEQP CPK LGD+VES VGAVLVDTGFDMNYVWKIMLSFLD IMS SGFQLSPIRD
Sbjct: 1331 ERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKIMLSFLDPIMSSSGFQLSPIRD 1390

Query: 1444 ITEFCQDCGWKLQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKL 1503
            I E CQ CGWKLQFN +K+EKY+SV+AEVKG++FH TASA NRRKKDAEKIAAN IL+KL
Sbjct: 1391 IIEVCQTCGWKLQFNPSKMEKYYSVEAEVKGDDFHATASAVNRRKKDAEKIAANSILTKL 1450

Query: 1504 KEKGFIPEVSSLEEILRSRKKMEPELIGFDETPSVSIDQADNGSGTIPGSEHSGAELDPC 1563
            K +GFIPEV+SLE+IL+S +KM+P+LIG+DETPSV++D+ D+      G E SGA+LDP 
Sbjct: 1451 KAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDRIDD------GLEFSGADLDPR 1510

Query: 1564 VNSEFSNSHSVRITRLSEAPEFSSVAAEEELKPST-----FGGTNLQ----------SSS 1623
            V+ E  +S   RITR+SE    SS AA E+LKPS         T+LQ          SSS
Sbjct: 1511 VHREVGDSRPFRITRMSETLASSSGAAGEQLKPSIPLEGHDSSTDLQSLSSSSSSSSSSS 1570

Query: 1624 GRSSKTTARSRLYEICAANHWNQPSFDCMNEEGPGHLKMFTYKVVLEIEEAPDTIFEFFG 1683
            GRS KTTARSRLYE+CA NHWN PSFDCMNEEGP HLKMFTYKVVLEIEEAP+TIFEFFG
Sbjct: 1571 GRSGKTTARSRLYEVCAINHWNHPSFDCMNEEGPSHLKMFTYKVVLEIEEAPNTIFEFFG 1630

Query: 1684 ASHSRKKAAAEHAAEAALWYLKKEGYWNETD 1696
            A H  KKAAAEHAAEAALWYLKKE Y  +TD
Sbjct: 1631 APHLTKKAAAEHAAEAALWYLKKERYLGDTD 1654

BLAST of Sed0002244 vs. NCBI nr
Match: XP_023515971.1 (dicer-like protein 4 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2650.5 bits (6869), Expect = 0.0e+00
Identity = 1359/1651 (82.31%), Postives = 1459/1651 (88.37%), Query Frame = 0

Query: 64   SDFIDNSKPSSLAVEKLMGLSLVGDPGAGSSSRASEKDPRRVARKYQLELCKKALEENII 123
            +D ++    SS +VE+ MGLS V D GAGSS R S KDPRR+ARKYQLELC+KALEENII
Sbjct: 11   ADSVNFQPTSSASVERFMGLSPVDDQGAGSSIRTSGKDPRRIARKYQLELCEKALEENII 70

Query: 124  VYLGTGCGKTHIAILLIYEMRNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVY 183
            VYLGTGCGKTHIAILLIYEM +LIRR QKGICVFLAPTVALVQQQAKVIEDSIDFKV+VY
Sbjct: 71   VYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIEDSIDFKVKVY 130

Query: 184  CGGFKGLNSHYDWEREMEEHEVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSD 243
            CGG K LNSHYDWEREMEE+EVFVMTP+ LLR+L+HCYIKMEFVELLIFDECHHAQVKSD
Sbjct: 131  CGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIKMEFVELLIFDECHHAQVKSD 190

Query: 244  HSYAQIMKVFYKGSVAKRPRIFGMTASPVVGKGTCNQQSLSKSINSLEKLLDAKVYSVED 303
            HSYA+IM+VFYK +  KRPRIFGMTASPVVGKG CNQQ+LSKSINSLEKLLDAKVYSVED
Sbjct: 191  HSYAEIMRVFYKANEHKRPRIFGMTASPVVGKGACNQQNLSKSINSLEKLLDAKVYSVED 250

Query: 304  REDLEAFVSSPLVKIYYYGPVANGSSSSFMTYCCQLEEVKRQCVVALGQMKGEHEGLLAT 363
            RE+LEAFVSSP  KIYYYGPVA GSSSSFMTYCC LE+VK QC+VALGQMKGEHEGLLAT
Sbjct: 251  REELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKCQCIVALGQMKGEHEGLLAT 310

Query: 364  KKLLLRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNDSLSDRYLN 423
            KKLL RMH NIIFCLENLGVWGALQASKILLS DNSERSELIEAAE NP +DS+SDRYLN
Sbjct: 311  KKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSELIEAAEANPKHDSISDRYLN 370

Query: 424  QAAEILASGCKIDGGMPDMLNVDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIV 483
            QAAEI ASGCKID G+ DMLNVDIL++P FSKKLLRLIGILSSF HR NMKCIIFVNRIV
Sbjct: 371  QAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGILSSFRHRPNMKCIIFVNRIV 430

Query: 484  IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGE 543
            IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMN ILTKFRSGELNLLIATKVGE
Sbjct: 431  IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHILTKFRSGELNLLIATKVGE 490

Query: 544  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDEN 603
            EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAF+VD GNEKE GLID F KDE 
Sbjct: 491  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFHKDEK 550

Query: 604  HMNREISSRSSNETFQSHEESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKF 663
             MNREISSRSS ETF  HEESIYRVVSTGASVTSG SISLLHEYCSKLPHD YFDPKPKF
Sbjct: 551  RMNREISSRSSIETFDGHEESIYRVVSTGASVTSGRSISLLHEYCSKLPHDTYFDPKPKF 610

Query: 664  FYYDDLEGTICHAILPSNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPM 723
            FY DDL GTICHAILPSNAPIPQ+VSR Q +  AAKKDACLKA+EELHKLGA +D+LLPM
Sbjct: 611  FYCDDLGGTICHAILPSNAPIPQIVSRPQPAGVAAKKDACLKAVEELHKLGALSDYLLPM 670

Query: 724  RDNAIEEELELASCDSDSSEDETSRRELHEMLVPTALKDSWTGSGNVILNCYQIKISPHP 783
            RDNA E ELEL S DSDSSEDETSRRELHEM+ P A K+SWTG G VIL CY IK +P+P
Sbjct: 671  RDNANEGELELDS-DSDSSEDETSRRELHEMISPAAFKESWTGFGYVILYCYHIKFTPNP 730

Query: 784  GDRIYKEFGLFVKAPLPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQE 843
             DRIYKEFGLFVKAPLPQEA R+DLELHLA  R VKVNL+PS VVELLE+EITQAE FQE
Sbjct: 731  EDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMPSRVVELLENEITQAECFQE 790

Query: 844  MFLKVILDRSEFVQDYVPLRNNVSGSISSASYLLLPMIFQDNEGSSLIDWKVIRRCLSSQ 903
            MFLKVILDRSEFV DY+PLRNNVS SISS SYLLLP+I  DNEGS  IDWKVIRRCLSSQ
Sbjct: 791  MFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHDNEGSVFIDWKVIRRCLSSQ 850

Query: 904  IFQNHACSAVRETASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSR 963
            IFQNHACS V+ETASSDTHL LYDGRRR  DIENSLVYVPYKREFFFVT+IVR KNGYS+
Sbjct: 851  IFQNHACSIVKETASSDTHLMLYDGRRRSSDIENSLVYVPYKREFFFVTNIVRRKNGYSQ 910

Query: 964  YKNSGSLSHFEHLLERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLID 1023
            YK+SGSLSHFEHL  RF IHLKYPEQPLLCAKPLF LHNLLH+R+  DS A  L E+ ID
Sbjct: 911  YKDSGSLSHFEHLKTRFGIHLKYPEQPLLCAKPLFSLHNLLHNRKQKDS-APYLEEYFID 970

Query: 1024 LPPELCQLKITGFTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRIL 1083
            + PELCQLKITGF+KDIGSSISLLPS MH LEN LVAIELKC LAAAFPA +EVT+NRIL
Sbjct: 971  IAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPAGAEVTANRIL 1030

Query: 1084 EALTTEKCQERISLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFK 1143
            EALTTEKCQERISLERLEILGD+FLKFAVAR+LFL HDKFDEGELTRRRSYLVKNINLFK
Sbjct: 1031 EALTTEKCQERISLERLEILGDSFLKFAVARYLFLAHDKFDEGELTRRRSYLVKNINLFK 1090

Query: 1144 LATRKNLQVYIRDQPFEPNQFYSLGRPCPRICNEETRKDIHSH----KNAKGGETKCSKG 1203
            LATRKNLQVYIRDQPFEP+QFY LGRPCPRICNEETR DIHSH     NAK  ETKCSKG
Sbjct: 1091 LATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRNDIHSHDNATNNAKASETKCSKG 1150

Query: 1204 HHWLHKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRL 1263
            HHWL KKTISDVVEALVGAFLVDSGFKAAIAFLKWIGI+VEFEASQVT+A +AS AY+ L
Sbjct: 1151 HHWLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIKVEFEASQVTNALVASKAYVLL 1210

Query: 1264 ADCISISALQDSLGHQFLYKGLLLQALIHPSYHRHGGGCYQRLEFLGDAVLDYLITSYLY 1323
            AD I+IS+LQ SLGH+FL+KGLLLQAL+HP+YH+HGGGCYQRLEFLGDAVLDYLI SYLY
Sbjct: 1211 ADTINISSLQSSLGHKFLHKGLLLQALVHPTYHKHGGGCYQRLEFLGDAVLDYLIMSYLY 1270

Query: 1324 SVYPKLKPGLVTDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIKSPPS 1383
            S YPKLKPGL+TDLRSVFV NEAFAN++VDRFFYKFL+CDS SLQ DIKSYV+FIK+PPS
Sbjct: 1271 SAYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLCDSASLQSDIKSYVDFIKAPPS 1330

Query: 1384 ERGSLEQPTCPKVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRD 1443
            ER SLEQP CPK LGD+VES VGAVLVDTGFDMNYVWKIMLSFLD IMS SGFQLSPIRD
Sbjct: 1331 ERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKIMLSFLDPIMSSSGFQLSPIRD 1390

Query: 1444 ITEFCQDCGWKLQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKL 1503
            I E CQ CGWKLQFN +K+EKY+SV+AEVKG++FH TASA NRRKKDAEKIAAN IL+KL
Sbjct: 1391 IIEVCQTCGWKLQFNPSKMEKYYSVEAEVKGDDFHATASAVNRRKKDAEKIAANSILTKL 1450

Query: 1504 KEKGFIPEVSSLEEILRSRKKMEPELIGFDETPSVSIDQADNGSGTIPGSEHSGAELDPC 1563
            K +GFIPEV+SLE+IL+S +KM+P+LIG+DETPSV++D+ D+      G E SGA+LDP 
Sbjct: 1451 KAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDRIDD------GLEFSGADLDPR 1510

Query: 1564 VNSEFSNSHSVRITRLSEAPEFSSVAAEEELKPST-----FGGTNLQ----------SSS 1623
            V+ E  +S   RITR+SE    SS AA E+LKPS         T+LQ          SSS
Sbjct: 1511 VHREVGDSRPFRITRMSETLASSSGAAGEQLKPSIPLEGHDSSTDLQSLSSSSSSSSSSS 1570

Query: 1624 GRSSKTTARSRLYEICAANHWNQPSFDCMNEEGPGHLKMFTYKVVLEIEEAPDTIFEFFG 1683
            GRS KTTARSRLYE+CA NHWN PSFDCMNEEGP HLKMFTYKVVLEIEEAP+TIFEFFG
Sbjct: 1571 GRSGKTTARSRLYEVCAINHWNHPSFDCMNEEGPSHLKMFTYKVVLEIEEAPNTIFEFFG 1630

Query: 1684 ASHSRKKAAAEHAAEAALWYLKKEGYWNETD 1696
            A H  KKAAAEHAAEAALWYLKKE Y  +TD
Sbjct: 1631 APHLTKKAAAEHAAEAALWYLKKERYLGDTD 1653

BLAST of Sed0002244 vs. ExPASy Swiss-Prot
Match: P84634 (Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2)

HSP 1 Score: 1640.6 bits (4247), Expect = 0.0e+00
Identity = 863/1620 (53.27%), Postives = 1126/1620 (69.51%), Query Frame = 0

Query: 84   SLVGDPGAGSSSRASEKDPRRVARKYQLELCKKALEENIIVYLGTGCGKTHIAILLIYEM 143
            SL    G    S   EKDPR++AR+YQ+ELCKKA EEN+IVYLGTGCGKTHIA++LIYE+
Sbjct: 102  SLFSAAGTDDPSPKMEKDPRKIARRYQVELCKKATEENVIVYLGTGCGKTHIAVMLIYEL 161

Query: 144  RNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVYCGGFKGLNSHYDWEREMEEH 203
             +L+   +K +C+FLAPTVALV+QQAKVI DS++FKV ++CGG + + SH +WERE+  +
Sbjct: 162  GHLVLSPKKSVCIFLAPTVALVEQQAKVIADSVNFKVAIHCGGKRIVKSHSEWEREIAAN 221

Query: 204  EVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSDHSYAQIMKVFYKGSVAKRPR 263
            EV VMTPQ LL +L HC+IKME + LLIFDECHHAQ +S+H YA+IMKVFYK    +RPR
Sbjct: 222  EVLVMTPQILLHNLQHCFIKMECISLLIFDECHHAQQQSNHPYAEIMKVFYKSESLQRPR 281

Query: 264  IFGMTASPVVGKGTCNQQSLSKSINSLEKLLDAKVYSVEDREDLEAFVSSPLVKIYYYGP 323
            IFGMTASPVVGKG+   ++LSKSINSLE LL+AKVYSVE    L+ FVSSPLVK+YYY  
Sbjct: 282  IFGMTASPVVGKGSFQSENLSKSINSLENLLNAKVYSVESNVQLDGFVSSPLVKVYYYRS 341

Query: 324  VANGSSSSFMTYCCQLEEVKRQCVVALGQMKGEH--EGLLATKKLLLRMHDNIIFCLENL 383
              + +S S + Y   LE++K++C+ +L  +   H  + LL+ K+LL R HDN+I+ L NL
Sbjct: 342  ALSDASQSTIRYENMLEDIKQRCLASLKLLIDTHQTQTLLSMKRLLKRSHDNLIYTLLNL 401

Query: 384  GVWGALQASKILLSGDNSERSELIEAAEGNPNNDSLSDRYLNQAAEILASGCKIDGGMPD 443
            G+WGA+QA+KI L+ D++ + E +    G      + D YL+ AAE L+SG   D    D
Sbjct: 402  GLWGAIQAAKIQLNSDHNVQDEPV----GKNPKSKICDTYLSMAAEALSSGVAKDENASD 461

Query: 444  MLNVDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIVIARSLSYILQNLKFLAYW 503
            +L++  L++P FS+KL++LI ILS F    +MKCIIFVNRIV AR+LS IL NL+ L  W
Sbjct: 462  LLSLAALKEPLFSRKLVQLIKILSVFRLEPHMKCIIFVNRIVTARTLSCILNNLELLRSW 521

Query: 504  KCDFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPE 563
            K DFLVG+ S L+SMSR++M  IL +F+S ELNLL+ATKVGEEGLDIQTCCLVIR+DLPE
Sbjct: 522  KSDFLVGLSSGLKSMSRRSMETILKRFQSKELNLLVATKVGEEGLDIQTCCLVIRYDLPE 581

Query: 564  TVSSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDENHMNREISSRSSNETFQSH 623
            TV+SFIQSRGRARMPQSEYAF+VD GNEKE  LI+ F+ +E+ MN EI+ RSS ET    
Sbjct: 582  TVTSFIQSRGRARMPQSEYAFLVDSGNEKEMDLIENFKVNEDRMNLEITYRSSEETCPRL 641

Query: 624  EESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKFFY--YDDLEGTICHAILP 683
            +E +Y+V  TGA ++ G SISLL++YCS+LPHDE+F PKP+F +   D+  GTIC   LP
Sbjct: 642  DEELYKVHETGACISGGSSISLLYKYCSRLPHDEFFQPKPEFQFKPVDEFGGTICRITLP 701

Query: 684  SNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPMRDNAIEEELELASCDS 743
            +NAPI ++ S +  S +AAKKDACLKA+ ELH LG  NDFLLP   + IE+EL     D 
Sbjct: 702  ANAPISEIESSLLPSTEAAKKDACLKAVHELHNLGVLNDFLLPDSKDEIEDELSDDEFDF 761

Query: 744  DSSEDE-TSRRELHEMLVPTALKDSWTGSGNVI-LNCYQIKISPHPGDRIYKEFGLFVKA 803
            D+ + E  SR +L+EM VP   K  W  S + + L+ Y I   PHP DRIYK+FG F+K+
Sbjct: 762  DNIKGEGCSRGDLYEMRVPVLFKQKWDPSTSCVNLHSYYIMFVPHPADRIYKKFGFFMKS 821

Query: 804  PLPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFVQ 863
            PLP EAE +D++LHLA  R V V + PSGV E   DEI  AE FQE+ LKV+ +R E + 
Sbjct: 822  PLPVEAETMDIDLHLAHQRSVSVKIFPSGVTEFDNDEIRLAELFQEIALKVLFERGELIP 881

Query: 864  DYVPLR-NNVSGSISSASYLLLPMIFQDNEGSSLIDWKVIRRCLSSQIFQNHACSAVRET 923
            D+VPL   + S +  S  YLLLP+   D E    +DW  IR CLSS IF+  +       
Sbjct: 882  DFVPLELQDSSRTSKSTFYLLLPLCLHDGESVISVDWVTIRNCLSSPIFKTPSVLVEDIF 941

Query: 924  ASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSRYKNSGSLSHFEHL 983
              S +HL L +G   + D++NSLV+  Y ++F+FV  I   +NG+S  K S + SH E +
Sbjct: 942  PPSGSHLKLANGCWNIDDVKNSLVFTTYSKQFYFVADICHGRNGFSPVKESSTKSHVESI 1001

Query: 984  LERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLIDLPPELCQLKITGF 1043
             + + + LK+P QPLL  KPL H+ NLLH+R   + E  +L E+ I++PPEL  LKI G 
Sbjct: 1002 YKLYGVELKHPAQPLLRVKPLCHVRNLLHNRMQTNLEPQELDEYFIEIPPELSHLKIKGL 1061

Query: 1044 TKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRILEALTTEKCQERIS 1103
            +KDIGSS+SLLPS MH +EN LVAIELK  L+A+ P  +EV+ +R+LEALTTEKC ER+S
Sbjct: 1062 SKDIGSSLSLLPSIMHRMENLLVAIELKHVLSASIPEIAEVSGHRVLEALTTEKCHERLS 1121

Query: 1104 LERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFKLATRKNLQVYIRD 1163
            LERLE+LGDAFLKFAV+RHLFL HD  DEGELTRRRS +V N NL +LA +KNLQVYIRD
Sbjct: 1122 LERLEVLGDAFLKFAVSRHLFLHHDSLDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRD 1181

Query: 1164 QPFEPNQFYSLGRPCPRICNEETRKDIHSHKNAKG------GETKCSKGHHWLHKKTISD 1223
            Q  +P QF++ G PC   C+E   K++HS     G      GE +CSKGHHWL+KKTI+D
Sbjct: 1182 QALDPTQFFAFGHPCRVTCDEVASKEVHSLNRDLGILESNTGEIRCSKGHHWLYKKTIAD 1241

Query: 1224 VVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRLADCISISALQD 1283
            VVEALVGAFLVDSGFK A+ FLKWIG+ V+FE+ QV DA +AS  YL L    ++  L++
Sbjct: 1242 VVEALVGAFLVDSGFKGAVKFLKWIGVNVDFESLQVQDACIASRRYLPLTTRNNLETLEN 1301

Query: 1284 SLGHQFLYKGLLLQALIHPSYHRHGGGCYQRLEFLGDAVLDYLITSYLYSVYPKLKPGLV 1343
             L ++FL+KGLL+QA IHPSY+RHGGGCYQRLEFLGDAVLDYL+TSY ++V+PKLKPG +
Sbjct: 1302 QLDYKFLHKGLLVQAFIHPSYNRHGGGCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQL 1361

Query: 1344 TDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIKSPPSERGSLEQPTCP 1403
            TDLRS+ V NEA AN++V     +FL C+S  L + I+ Y NF+ S P   G  E P CP
Sbjct: 1362 TDLRSLSVNNEALANVAVSFSLKRFLFCESIYLHEVIEDYTNFLASSPLASGQSEGPRCP 1421

Query: 1404 KVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRDITEFCQDCGWK 1463
            KVLGD+VES +GA+ +D GF++N+VW +MLSFLD + + S  Q+SPI+++ E CQ   W 
Sbjct: 1422 KVLGDLVESCLGALFLDCGFNLNHVWTMMLSFLDPVKNLSNLQISPIKELIELCQSYKWD 1481

Query: 1464 LQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKLKEKGFIPEVSS 1523
             + +ATK +  F+V+ +V  N    T SA  R K++  K AA L+++ LK    I     
Sbjct: 1482 REISATKKDGAFTVELKVTKNGCCLTVSATGRNKREGTKKAAQLMITNLKAHENITTSHP 1541

Query: 1524 LEEILRSRKKMEPELIGFDETPSVSIDQADNGSGTIPGSEHSGAELDPCVNSEFSNSHSV 1583
            LE++L++  + E +LIG++E P   +D        +   E  G       NSE S+S+ +
Sbjct: 1542 LEDVLKNGIRNEAKLIGYNEDPIDVVDLVGLDVENLNILETFGG------NSERSSSYVI 1601

Query: 1584 RITRLSEAPEFSSVAAEEELKPSTFGGTNLQSSSGRSSKTTARSRLYEICAANHWNQPSF 1643
            R   L +AP       E+ L         +  + G SSK TA+S L+E C AN W  P F
Sbjct: 1602 R-RGLPQAPS----KTEDRLPQKA-----IIKAGGPSSK-TAKSLLHETCVANCWKPPHF 1661

Query: 1644 DCMNEEGPGHLKMFTYKVVLEIEEAPDTIFEFFGASHSRKKAAAEHAAEAALWYLKKEGY 1691
            +C  EEGPGHLK F YKV+LE+E+AP+   E +G + + KK AAEHAA+AA+W LK  G+
Sbjct: 1662 ECCEEEGPGHLKSFVYKVILEVEDAPNMTLECYGEARATKKGAAEHAAQAAIWCLKHSGF 1700

BLAST of Sed0002244 vs. ExPASy Swiss-Prot
Match: A7LFZ6 (Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL4 PE=2 SV=1)

HSP 1 Score: 1436.4 bits (3717), Expect = 0.0e+00
Identity = 798/1664 (47.96%), Postives = 1072/1664 (64.42%), Query Frame = 0

Query: 81   MGLSLVGDPGAG----SSSRASEKDPRRVARKYQLELCKKALEENIIVYLGTGCGKTHIA 140
            MG +    P A     SS+R   KDPR +ARKYQL+LCK+A+EENIIVYLGTGCGKTHIA
Sbjct: 1    MGDAAAAAPAAAAAGPSSTRGEPKDPRTIARKYQLDLCKRAVEENIIVYLGTGCGKTHIA 60

Query: 141  ILLIYEMRNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVYCGGFKGLNSHYDW 200
            +LLIYE+ +LIR+  + +C+FLAPT+ LV+QQA VI  S DFKV+ Y G  K    H +W
Sbjct: 61   VLLIYELGHLIRKPSREVCIFLAPTIPLVRQQAVVIASSTDFKVQCYYGNGKNSRDHQEW 120

Query: 201  EREMEEHEVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSDHSYAQIMKVFY-K 260
            E +M E EV VMTPQ LL+SL HC+IKM  + LLI DECHHAQ +  H YAQIMK FY  
Sbjct: 121  ENDMREFEVLVMTPQILLQSLRHCFIKMNSIALLILDECHHAQPQKRHPYAQIMKEFYNS 180

Query: 261  GSVAKRPRIFGMTASPVVGKGT--------------------------CNQQSLSKSINS 320
             SV K PR+FGMTASP++GKG                            N+ + +K INS
Sbjct: 181  NSVEKFPRVFGMTASPIIGKGVMPSHSFTEKGGRSPCQPLIFFLPKGGSNKLNYTKCINS 240

Query: 321  LEKLLDAKVYSVEDREDLEAFVSSPLVKIYYYGPVANGSSSSFMTYCC-QLEEVKRQCVV 380
            LE+LL AKV SV D E+LE+ V+SP +++Y+YGPV   + S+  T C  +L+ +K Q   
Sbjct: 241  LEELLHAKVCSV-DNEELESVVASPDMEVYFYGPV---NHSNLTTICIKELDSLKLQSER 300

Query: 381  ALGQMKGEHEGLLATKKLLLRMHDNIIFCLENLGVWGALQASKILLS--GDNSERSELIE 440
             L     + +      K L R+H+NIIFCL+ LG +GALQA++  LS  GD  +R E+  
Sbjct: 301  MLRASLCDFKDSQKKLKSLWRLHENIIFCLQELGSFGALQAARTFLSFDGDKLDRREV-- 360

Query: 441  AAEGNPNNDSLSDRYLNQAAEILASGCKIDGGMPDMLNVDILEDPFFSKKLLRLIGILSS 500
              + N +  S +  YLN A  IL+   K DG      +++ LE+PFFS K   LI +LS 
Sbjct: 361  --DLNGSTSSFAHHYLNGATSILSRN-KTDGSHAGSFDLEKLEEPFFSNKFSVLINVLSR 420

Query: 501  FGHRLNMKCIIFVNRIVIARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNRILT 560
            +G + NMKCI+FV RI +AR++S ILQNLK L +WKC+FLVG HS  ++MSR  M+ I+ 
Sbjct: 421  YGLQENMKCIVFVKRITVARAISNILQNLKCLEFWKCEFLVGCHSGSKNMSRNKMDAIVQ 480

Query: 561  KFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFIVDR 620
            +F SGE+NLL+AT VGEEGLDIQTCCLV+RFDLPETV+SFIQSRGRARM +S+Y  +++R
Sbjct: 481  RFSSGEVNLLVATSVGEEGLDIQTCCLVVRFDLPETVASFIQSRGRARMTKSKYVVLLER 540

Query: 621  GNEKERGLIDGFQKDENHMNREISSRSSNETFQSHEESIYRVVSTGASVTSGLSISLLHE 680
             N+    L++G+   E+ MN EI SR+SN+ F   EE+IY+V +TGAS+++  S+SLLH 
Sbjct: 541  ENQSHEKLLNGYIAGESIMNEEIDSRTSNDMFDCLEENIYQVDNTGASISTACSVSLLHC 600

Query: 681  YCSKLPHDEYFDPKPKFFYYDDLEGTICHAILPSNAPIPQVVSRVQFSRDAAKKDACLKA 740
            YC  LP D +F P P FFY D +EG IC  ILP NA   Q   +   S+D AK+DACLKA
Sbjct: 601  YCDNLPRDMFFTPSPVFFYIDGIEGIICRLILPPNAAFRQADGQPCLSKDEAKRDACLKA 660

Query: 741  IEELHKLGAFNDFLLPMRDNAIEEELELASCDSDSSEDETSRRELHEMLVPTALKDSWTG 800
              +LHKLGA  DFLLP   +   +     +  ++  ED++ R ELHEML+P  LK S   
Sbjct: 661  CVKLHKLGALTDFLLPGPGSRKNKVSVTNNSSNNKVEDDSLREELHEMLIPAVLKPSGLK 720

Query: 801  SGNVI-LNCYQIKISPHPGDRIYKEFGLFVKAPLPQEAERIDLELHLARSRYVKVNLVPS 860
              ++  L+ Y +K  P P DR Y+ FGLFV  PLP EAE + ++LHLAR R VK  +   
Sbjct: 721  LDSLSNLHFYYVKFIPIPEDRRYQMFGLFVINPLPVEAETLQVDLHLARGRIVKAGIKHL 780

Query: 861  GVVELLEDEITQAESFQEMFLKVILDRSEFVQDYVPLRNNVSGSISSASYLLLPMIFQDN 920
            G +   ++++  A  FQEM LK++LDRSEF   +V L N+V+  I+S  YLLLP+  +  
Sbjct: 781  GKIAFEKEKMMLAHKFQEMCLKILLDRSEFTSPHVKLGNDVTLEINSTFYLLLPIKQKCY 840

Query: 921  EGSSLIDWKVIRRCLSSQIFQNHACSAVRETASSDTHLTLYDGRRRLCDIENSLVYVPYK 980
                +IDW  + RCLSS IF++    +V  + SS+  L L DG     D+  S+V+ P+ 
Sbjct: 841  GDRFMIDWPAVERCLSSPIFKDPIDVSVHASYSSNESLRLLDGIFSKTDVVGSVVFSPHN 900

Query: 981  REFFFVTHIVRSKNGYSRYKNSGSLSHFEHLLERFDIHLKYPEQPLLCAKPLFHLHNLLH 1040
              FFFV  I+   N +S +  +   ++ EH  ERF I L +PEQPLL AK +F+L NLLH
Sbjct: 901  NIFFFVDGILDEINAWSEHSGA---TYAEHFKERFRIELSHPEQPLLKAKQIFNLRNLLH 960

Query: 1041 DR--RLNDSEACDLIEHLIDLPPELCQLKITGFTKDIGSSISLLPSFMHHLENFLVAIEL 1100
            +R     +SE  +L+EH ++LPPELC LK+ GF+KD+GSS+SLLPS M+ LEN LVAIEL
Sbjct: 961  NRLPETTESEGRELLEHFVELPPELCSLKVIGFSKDMGSSLSLLPSLMYRLENLLVAIEL 1020

Query: 1101 KCTLAAAFPAASEVTSNRILEALTTEKCQERISLERLEILGDAFLKFAVARHLFLTHDKF 1160
            K  + ++FP AS+++++ ILEALTTEKC ERISLER E+LGDAFLK+ V RH F+T++  
Sbjct: 1021 KDVMLSSFPEASQISASGILEALTTEKCLERISLERFEVLGDAFLKYVVGRHKFITYEGL 1080

Query: 1161 DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPNQFYSLGRPCPRICNEETRKDI 1220
            DEG+LTRRRS +V N +L++L+ RK LQVYIRDQ FEP QF++ GRPC  +CN +    +
Sbjct: 1081 DEGQLTRRRSDVVNNSHLYELSIRKKLQVYIRDQQFEPTQFFAPGRPCKVVCNTDVEVRL 1140

Query: 1221 HS----HKNAKGGETKCSKGHHWLHKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQV 1280
            H       N +    +C++ HHWLH+K I+DVVE+L+GAFLV+ GFKAA AFL WIGI V
Sbjct: 1141 HQMDIHPDNRENCNLRCTRSHHWLHRKVIADVVESLIGAFLVEGGFKAAFAFLHWIGIDV 1200

Query: 1281 EFEASQVTDARMASNAYLRLADCISISALQDSLGHQFLYKGLLLQALIHPSYHRHGGGCY 1340
            +F    +     +S+  L L D   I+ L++ +G++F +KGLLLQA +HPS+ +H GGCY
Sbjct: 1201 DFNNPALYRVLDSSSINLSLMDYTDIAGLEELIGYKFKHKGLLLQAFVHPSFSQHSGGCY 1260

Query: 1341 QRLEFLGDAVLDYLITSYLYSVYPKLKPGLVTDLRSVFVMNEAFANISVDRFFYKFLICD 1400
            QRLEFLGDAVL+Y+ITSYLYS YP +KPG +TDLRS+ V N++ A  +V++  +K LI D
Sbjct: 1261 QRLEFLGDAVLEYVITSYLYSTYPDIKPGQITDLRSLAVGNDSLAYAAVEKSIHKHLIKD 1320

Query: 1401 STSLQKDIKSYVNFIKSPPSERGSLEQPTCPKVLGDMVESIVGAVLVDTGFDMNYVWKIM 1460
            S  L   I  +  ++K   SE+  LE+P CPK LGD+VES +GAVL+D+GF++NYVWK+M
Sbjct: 1321 SNHLTSAISKFEMYVKLSNSEKDLLEEPACPKALGDIVESCIGAVLLDSGFNLNYVWKVM 1380

Query: 1461 LSFLDSIMSFSGFQLSPIRDITEFCQDCGWKLQF-NATKVEKYFSVKAEVKGNNFHETAS 1520
            L  L  +++F+    +P+R++ E CQ  G++L      K +  + VK EV   +     +
Sbjct: 1381 LMLLKPVLTFANMHTNPMRELRELCQCHGFELGLPKPMKADGEYHVKVEVNIKSKIIICT 1440

Query: 1521 AANRRKKDAEKIAANLILSKLKEKGFIPEVSSLEEILRSRKKMEPELIGFDETPSVSIDQ 1580
            AANR  K A K AA   LSKLK  G+     SLEEIL   +K E ELIG++E P   ID 
Sbjct: 1441 AANRNSKAARKFAAQETLSKLKNYGYKHRNKSLEEILIVARKRESELIGYNEDP---IDV 1500

Query: 1581 ADNGSGTIPGSEHSGAELDPCVNSEFSNSHSVRITR---LSEAPEFSSVAAEEELKPSTF 1640
              + S  +  S H   E  P  N+E S + S +              +     + K +T 
Sbjct: 1501 EADISVKMK-SPHIHEENIPFQNTETSFTRSSKFHNQIIAGSGKHDVNNGRNNQPKLATQ 1560

Query: 1641 GGTNLQSSSGRSSK---------TTARSRLYEICAANHWNQPSFDCMNEEGPGHLKMFTY 1691
             G     ++ +S+K          TARS L+E+CAAN+W  P F    EEGP HL+ FTY
Sbjct: 1561 SGRLPSEATEKSNKKVYHGDMVHKTARSFLFELCAANYWKPPEFKLCKEEGPSHLRKFTY 1620

BLAST of Sed0002244 vs. ExPASy Swiss-Prot
Match: Q5N870 (Endoribonuclease Dicer homolog 3a OS=Oryza sativa subsp. japonica OX=39947 GN=DCL3A PE=2 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 1.6e-199
Identity = 519/1669 (31.10%), Postives = 821/1669 (49.19%), Query Frame = 0

Query: 107  RKYQLELCKKALEENIIVYLGTGCGKTHIAILLIYEMRNLIRRSQKG-ICVFLAPTVALV 166
            R+YQL++ + A+  N I  L TG GKT IA++LI E   + R    G + +FLAPTV LV
Sbjct: 33   RRYQLDVYEVAMRRNTIAMLDTGAGKTMIAVMLIKEFGKINRTKNAGKVIIFLAPTVQLV 92

Query: 167  QQQAKVIEDSIDFKVRVYCGGFKGLNSHYD--WEREMEEHEVFVMTPQTLLRSLNHCYIK 226
             QQ +VIE   DF+V  Y G  KG++      W+ ++ +++V VMTPQ  L++L + ++ 
Sbjct: 93   TQQCEVIEIHTDFEVEQYYGA-KGVDQWTGPRWQEQISKYQVMVMTPQVFLQALRNAFLI 152

Query: 227  MEFVELLIFDECHHAQVKSDHSYAQIMKVFYKGSVAKRPRIFGMTASPVVGKGTCNQQSL 286
            ++ V L+IFDECHHA    +H Y +IMK FY  S  K P +FGMTASPV+ KG  +    
Sbjct: 153  LDMVSLMIFDECHHA--TGNHPYTRIMKEFYHKSEHK-PSVFGMTASPVIRKGISSHLDC 212

Query: 287  SKSINSLEKLLDAKVYSVEDREDLEAFVSSPLVKIYYYG--PVA-NGSSSSFMTYCCQLE 346
                  LE LLDAK+Y+V DRE++E  V S      YY   PV     S      C + +
Sbjct: 213  EGQFCELENLLDAKIYTVSDREEIEFCVPSAKEMCRYYDSKPVCFEDLSEELGVLCSKYD 272

Query: 347  EVKRQCVVALGQMKGEHEGLL-ATKKLLLRMHDNIIFCLENLGVWGALQASKILLSGDNS 406
             +  +       M  + + +   +K+ L +    I +CL+++G+  A +A+KI +     
Sbjct: 273  ALITELQNKRSDMYKDADDITKESKRRLSKSIAKICYCLDDVGLICASEATKICIERGQE 332

Query: 407  E--RSELIEAAEGNPN---------NDSLSDRYLNQAAEILASGCKIDGGMPDMLN---- 466
            +    E+++A +   +         N +L  ++  +A  ++    ++  G+  +LN    
Sbjct: 333  KGWLKEVVDATDQQTDANGSRLFAENSALHMKFFEEALHLIDK--RLQQGIDMLLNSESG 392

Query: 467  -VDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIVIARSLSYILQNLKFLAYWKC 526
             V+  +  + S KL  LI I  SF +  + +C+IFV+R + AR +  +++ +  LA++  
Sbjct: 393  CVEAAKTGYISPKLYELIQIFHSFSNSRHARCLIFVDRKITARVIDRMIKKIGHLAHFTV 452

Query: 527  DFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETV 586
             FL G  S + +++ K     L  FRSG++NLL  T V EEG+ +  C  VIRFDLP T 
Sbjct: 453  SFLTGGRSSVDALTPKMQKDTLDSFRSGKVNLLFTTDVAEEGIHVPECSCVIRFDLPRTT 512

Query: 587  SSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDENHMNREISSRSSNET---FQS 646
             S++QSRGRAR   S+Y  +++RGN K+  LI    + E  M +  SSR S      F  
Sbjct: 513  RSYVQSRGRARQEDSQYILMIERGNVKQNDLISAIVRSETSMVKIASSRESGNLSPGFVP 572

Query: 647  HEE-SIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKFFYYDDLEGTICHAILP 706
            +EE + Y V +TGA VT+  SIS+++ YC KLP D+ + PKP F +    +G +C   LP
Sbjct: 573  NEEINEYHVGTTGAKVTADSSISIVYRYCEKLPQDKCYSPKPTFEFTHHDDGYVCTLALP 632

Query: 707  SNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPMRDNAIEEELELASCDS 766
             +A +  +V     +   AK+  CL A ++LH+LGA +D L    ++ +    E+ S + 
Sbjct: 633  PSAVLQILVGPKARNMHKAKQLVCLDACKKLHELGALDDHLCLSVEDPVP---EIVSKNK 692

Query: 767  DSSEDETSRRELHEMLVPTALKDSWTGSGNVI-LNCYQIKISPHPGDRIYKEFGLFVKAP 826
             +    T R+ELH      A   +W      + L  Y++        +IY EF L + A 
Sbjct: 693  GTGIGTTKRKELHGTTRIHAWSGNWVSKKTALKLQSYKMNFVCDQAGQIYSEFVLLIDAT 752

Query: 827  LPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFVQD 886
            LP E   ++++L+L   + VK ++   G++EL   ++ QA+ FQ +    +  +  F + 
Sbjct: 753  LPDEVATLEIDLYL-HDKMVKTSVSSCGLLELDAQQMEQAKLFQGLLFNGLFGKL-FTRS 812

Query: 887  YVP------LRNNVSGSI--SSASYLLLPMIFQDNEGSSLIDWKVIRRC-----LSSQIF 946
             VP      + N     +  +++ YLLLP      + +  I+W VI        L  +I+
Sbjct: 813  KVPNAPREFILNKEDTFVWNTASVYLLLP-TNPSFDSNVCINWSVIDAAATAVKLMRRIY 872

Query: 947  QNHACSAVRETASSDTHLTLYDGRRRLC---DIENSLVYVPYKREFFFVTHIVR-----S 1006
              +    +    S      L     + C    +++ +V   +  + +    I       +
Sbjct: 873  SENKRELLGIFDSDQNVGDLIHLANKSCKANSLKDMVVLAVHTGKIYTALDITELSGDSA 932

Query: 1007 KNGYSRYKNSGSLSHFEHLLERFDIHLKYPEQPLLCAKPLFHLHNLLH----------DR 1066
             +G S  K     +  E+  +++ I L++P QPLL  KP  + HNLL           + 
Sbjct: 933  FDGASDKKECKFRTFAEYFKKKYGIVLRHPSQPLLVLKPSHNPHNLLSSKFRDEGNVVEN 992

Query: 1067 RLNDSEACDLIEHLIDLPPELCQLKITGFTKDIGSSISLLPSFMHHLENFLVAIELKCTL 1126
              N +   +   + + +PPEL  L       +I  S  L P+ M+ +E+  +A +L+  +
Sbjct: 993  MSNGTPVVNKTSNRVHMPPEL--LIPLDLPVEILRSFYLFPALMYRIESLTLASQLRSEI 1052

Query: 1127 AAAFPAASEVTSNRILEALTTEKCQERISLERLEILGDAFLKFAVARHLFLTHDKFDEGE 1186
                 + S ++S  ILEA+TT +C E  S+ERLE+LGD+ LK+AV+ HLFL     DEG+
Sbjct: 1053 GY---SDSNISSFLILEAITTLRCSEDFSMERLELLGDSVLKYAVSCHLFLKFPNKDEGQ 1112

Query: 1187 LTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPNQFYSLG----RPCPRIC---NEETR 1246
            L+  R +++ N  L+KL   +N+Q Y+RD  F+P ++ + G    RP P  C   +E   
Sbjct: 1113 LSSIRCHMICNATLYKLGIERNVQGYVRDAAFDPRRWLAPGQLSIRPSPCECPVKSEVVT 1172

Query: 1247 KDIH--SHKNAKGGETKCSKGHHWLHKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQ 1306
             DIH    K    G+  C KGH W+  KTI+D VEA++GA+    G +AA+A LKW+GI 
Sbjct: 1173 DDIHIIDDKAIVLGKA-CDKGHRWMCSKTIADCVEAIIGAYYAGGGLRAAMAVLKWLGIG 1232

Query: 1307 VEFEASQVTDARMASNAYLRLADCISISALQDSLGHQFLYKGLLLQALIHPSYHRHGGG- 1366
             E E   +  A ++++    L        L+  LG+ F  KGLL++AL HPS    G   
Sbjct: 1233 AEIEEDLIVQAILSASVQTYLPKDNVFEMLEAKLGYSFSVKGLLVEALTHPSQQELGAKY 1292

Query: 1367 CYQRLEFLGDAVLDYLITSYLYSVYPKLKPGLVTDLRSVFVMNEAFANISVDRFFYKFLI 1426
            CY+RLEFLGDAVLD L+T YL++ +     G +TDLRS  V NE FA ++V   F+ FL 
Sbjct: 1293 CYERLEFLGDAVLDILLTRYLFNSHKDTNEGELTDLRSASVNNENFAQVAVKHNFHHFLQ 1352

Query: 1427 CDSTSLQKDIKSYVNFIKSPPSERGSLEQ---PTCPKVLGDMVESIVGAVLVDTGFDMNY 1486
              S  L   I  YVN ++    ++  L     P  PKVLGD+VESI GA+L+DT  D++ 
Sbjct: 1353 HSSGLLLDQITEYVNRLEGSSMDKVELLSDGLPKGPKVLGDIVESIAGAILLDTKLDLDV 1412

Query: 1487 VWKIMLSFLDSIMSFSGFQLSPIRDITEFCQDCGWKLQFNATKVEKYFSVKAEVKGNNFH 1546
            VW I    L  I++    +L P R++ E+C   G+ +  N            +V+     
Sbjct: 1413 VWGIFEPLLSPIVTPENLELPPYRELIEWCGKHGYFVGINCRDQGDTVVATLDVQLKEVL 1472

Query: 1547 ETASAANRRKKDAEKIAANLILSKLKEKGFIPEVSSLEEILRSRKKMEPELIGFDETPSV 1606
                  ++++KDA+  A++L+L  L+EKG I                             
Sbjct: 1473 LVRQGFSKKRKDAKAHASSLLLKDLEEKGLI----------------------------- 1532

Query: 1607 SIDQADNGSGTIPGSEHSGAELDPCVNSEFSNSHSVRITRLSEAPEFSSVAAEEELKPST 1666
                  N S T    +H G+       + F+N H   +   +  P     AA+       
Sbjct: 1533 ---IPKNASKTEQFEKHCGS------TNPFNNLHVDAMDTQTPKPTKEKNAADSRNISDP 1592

Query: 1667 FGGTNLQSSSGRSSKTTARSRLYEICAANHWN---------QPSFD--CMNEEGPGHL-- 1690
              G  L     ++SK   R  LYE+C    W          QPSF   C +  G      
Sbjct: 1593 LLGKPLHVIV-KTSKGGPRIALYELCKKLQWPMPTMESEKVQPSFSSVCSSPGGSSQKAT 1644

BLAST of Sed0002244 vs. ExPASy Swiss-Prot
Match: Q3EBC8 (Endoribonuclease Dicer homolog 2 OS=Arabidopsis thaliana OX=3702 GN=At3g03300 PE=1 SV=2)

HSP 1 Score: 664.1 bits (1712), Expect = 4.3e-189
Identity = 469/1430 (32.80%), Postives = 759/1430 (53.08%), Query Frame = 0

Query: 102  PRRVARKYQLELCKKALEENIIVYLGTGCGKTHIAILLIYEMRNLIRRSQKGICVFLAPT 161
            P   AR YQ+E  +KA+++N IV+L TG GKT IAI+L+     L R+     CVFL P 
Sbjct: 20   PLHFARSYQVEALEKAIKQNTIVFLETGSGKTLIAIMLLRSYAYLFRKPSPCFCVFLVPQ 79

Query: 162  VALVQQQAKVIEDSIDFKVRVYCGGFKGLN--SHYDWEREMEEHEVFVMTPQTLLRSLNH 221
            V LV QQA+ ++   D KV +Y G   G++      W++E++++EV VMTP  LL +L H
Sbjct: 80   VVLVTQQAEALKMHTDLKVGMYWGDM-GVDFWDSSTWKQEVDKYEVLVMTPAILLDALRH 139

Query: 222  CYIKMEFVELLIFDECHHAQVKSDHSYAQIMKVFY----KGSVAKRPRIFGMTASPVVGK 281
             ++ +  +++LI DECHHA  K  H YA IM+ FY        +  PRIFGMTAS V  K
Sbjct: 140  SFLSLSMIKVLIVDECHHAGGK--HPYACIMREFYHKELNSGTSNVPRIFGMTASLVKTK 199

Query: 282  GTCNQQSLSKSINSLEKLLDAKVYSVEDREDLEAFV--SSPLVKIYYYGPVANGSSSSFM 341
            G  N  S  K I+ LE L+++KVY+ E+   L  FV  S+P  K Y +  + +   +S +
Sbjct: 200  GE-NLDSYWKKIHELETLMNSKVYTCENESVLAGFVPFSTPSFKYYQHIKIPSPKRASLV 259

Query: 342  TYCCQLEEVKRQCVVALGQMKGEHEGLLATKKLLLRMHDNIIFCLENLGVWGALQASKIL 401
                +LE +  +  ++LG +      + + +K LLR+   + +CL++LG+  A +A++ L
Sbjct: 260  E---KLERLTIKHRLSLGTLDLNSSTVDSVEKRLLRISSTLTYCLDDLGILLAQKAAQSL 319

Query: 402  LSGDNSERSELIEAAEGNPNNDSLSDRYLNQAA-EILASGCKIDGGMPDMLNVDI----- 461
             +  N    +     E N  + +L  ++ + A+ E LA        +P  LN  +     
Sbjct: 320  SASQN----DSFLWGELNMFSVALVKKFCSDASQEFLAE-------IPQGLNWSVANING 379

Query: 462  -LEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIVIARSL-SYILQNLKFLAYWKCDF 521
              E    + K + LI  L  +    N++CIIFV+R++ A  L S + + L     WK  +
Sbjct: 380  NAEAGLLTLKTVCLIETLLGYSSLENIRCIIFVDRVITAIVLESLLAEILPNCNNWKTKY 439

Query: 522  LVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSS 581
            + G +S L++ +RK  N I+  FR G +N+++AT + EEGLD+Q+C LVIRFD    + S
Sbjct: 440  VAGNNSGLQNQTRKKQNEIVEDFRRGLVNIIVATSILEEGLDVQSCNLVIRFDPASNICS 499

Query: 582  FIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDENHMNREISSRS---SNETFQSHE 641
            FIQSRGRARM  S+Y  +V+ G+   +  +  +      M  E    S           +
Sbjct: 500  FIQSRGRARMQNSDYLMMVESGDLLTQSRLMKYLSGGKRMREESLDHSLVPCPPLPDDSD 559

Query: 642  ESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKFFYYDDLEGTICHAILPSNA 701
            E ++RV STGA+VT   S+SL++ YCS+LP DEYF P P+F    D +G+ C   LP + 
Sbjct: 560  EPLFRVESTGATVTLSSSVSLIYHYCSRLPSDEYFKPAPRFDVNKD-QGS-CTLYLPKSC 619

Query: 702  PIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPMRDNAIEEELELASCDSDSS 761
            P+ +V  + + +    K+  CLKA  +LHK+GA +D L+P        ++ +A   S   
Sbjct: 620  PVKEV--KAEANNKVLKQAVCLKACIQLHKVGALSDHLVP--------DMVVAETVSQKL 679

Query: 762  EDETSRRELHEMLVPTALKDSWTGSGNVILNCYQIKISPH-PGDRIYKEFGLFVKAPLPQ 821
            E      E      P  L   ++       + Y I++ P+ P +    +  L  +  L  
Sbjct: 680  EKIQYNTE-QPCYFPPELVSQFSAQPETTYHFYLIRMKPNSPRNFHLNDVLLGTRVVLED 739

Query: 822  EAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFVQDYVP 881
            +       L   R   + V L   G   L ++E+     FQ    +V+LD S  V++ + 
Sbjct: 740  DIGNTSFRLEDHRGT-IAVTLSYVGAFHLTQEEVLFCRRFQITLFRVLLDHS--VENLME 799

Query: 882  LRNNVSGSISSA-SYLLLPMIFQDNEGSSLIDWKVIR--RCLSSQIFQNHA-CSAVRETA 941
              N +      A  YLL+P     +  +SLIDW+VIR     S ++ + H  CS    T 
Sbjct: 800  ALNGLHLRDGVALDYLLVPS--THSHETSLIDWEVIRSVNLTSHEVLEKHENCS----TN 859

Query: 942  SSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYS--RYKNSGSLSHFEH 1001
             +   L   DG    C ++N+LVY P+    +    ++ + NG S    +NSG  ++ E+
Sbjct: 860  GASRILHTKDGLFCTCVVQNALVYTPHNGYVYCTKGVLNNLNGNSLLTKRNSGDQTYIEY 919

Query: 1002 LLERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLIDLPPELCQLKITG 1061
              ER  I L + ++PLL  + +F LH+ LH  +    +  D     ++LPPELC + ++ 
Sbjct: 920  YEERHGIQLNFVDEPLLNGRHIFTLHSYLHMAKKKKEKEHD--REFVELPPELCHVILSP 979

Query: 1062 FTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRILEALTTEKCQERI 1121
             + D+  S + +PS M  +E+ L+A  LK ++         + + ++LEA+TT+KC+++ 
Sbjct: 980  ISVDMIYSYTFIPSVMQRIESLLIAYNLKKSIPKV-----NIPTIKVLEAITTKKCEDQF 1039

Query: 1122 SLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFKLATRKNLQVYIR 1181
             LE LE LGD+FLK+AV + LF       EG L+ ++  ++ N+ L +   ++ LQ +IR
Sbjct: 1040 HLESLETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVMLCQFGCQQKLQGFIR 1099

Query: 1182 DQPFEPNQFYSLGRPCP--RICNEETRKDIHSHKNAKGGETKCSKGHHWLHKKTISDVVE 1241
            D+ FEP  +   G+      + N+   +  + +  ++            L +K+++DVVE
Sbjct: 1100 DECFEPKGWMVPGQSSAAYSLVNDTLPESRNIYVASRRN----------LKRKSVADVVE 1159

Query: 1242 ALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRLADCISISALQDSLG 1301
            +L+GA+L + G  AA+ F+ W+GI+V+F     T  ++  ++ ++    +++  ++  L 
Sbjct: 1160 SLIGAYLSEGGELAALMFMNWVGIKVDF-----TTTKIQRDSPIQAEKLVNVGYMESLLN 1219

Query: 1302 HQFLYKGLLLQALIHPSYHR-HGGGCYQRLEFLGDAVLDYLITSYLYSVYPKLKPGLVTD 1361
            + F  K LL++AL H SY       CYQRLEFLGD+VLDYLIT +LY  YP L PGL+TD
Sbjct: 1220 YSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLSPGLLTD 1279

Query: 1362 LRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIK--SPPSERGSLEQPTCP 1421
            +RS  V NE +A ++V    +K ++  S  L K I   V+  +  S  S  G     + P
Sbjct: 1280 MRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWESDISFP 1339

Query: 1422 KVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRDITEFCQDCGWK 1481
            KVLGD++ES+ GA+ VD+G++   V+  +   L  +++    +L P+R++TE CQ   W+
Sbjct: 1340 KVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELCQK--WQ 1383

Query: 1482 LQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKLK 1501
             + +  K    F+V+ + K  +F  TA A++  KK A+K+A   +L+ LK
Sbjct: 1400 FELSKAKDFDSFTVEVKAKEMSFAHTAKASD--KKMAKKLAYKEVLNLLK 1383

BLAST of Sed0002244 vs. ExPASy Swiss-Prot
Match: Q7XD96 (Endoribonuclease Dicer homolog 3b OS=Oryza sativa subsp. japonica OX=39947 GN=DCL3B PE=3 SV=2)

HSP 1 Score: 632.9 bits (1631), Expect = 1.1e-179
Identity = 506/1653 (30.61%), Postives = 791/1653 (47.85%), Query Frame = 0

Query: 111  LELCKKALEENIIVYLGTGCGKTHIAILLIYEMRNLIRRSQ--KGICVFLAPTVALVQQQ 170
            +E+ + AL  N I  L TG GKT +A++L  E    +R  +  + I VFLAPTV LV QQ
Sbjct: 44   VEVFEAALRGNTIAVLDTGSGKTMVAVMLAREHARRVRAGEAPRRIVVFLAPTVHLVHQQ 103

Query: 171  AKVIEDSIDFKVRVYCGGFK-GLNSHYDWEREMEEHEVFVMTPQTLLRSLNHCYIKMEFV 230
             +VI +  D  V +  G  + G      W+ E+  +E+ VMTPQ LL +L H ++ M  V
Sbjct: 104  FEVIREYTDLDVMMCSGASRVGEWGADHWKEEVGRNEIVVMTPQILLDALRHAFLTMSAV 163

Query: 231  ELLIFDECHHAQVKSDHSYAQIMKVFYKGSVAKRPRIFGMTASPVVGKGTCNQQSLSKSI 290
             LLIFDECH A     H YA+IMK FY GS   RP +FGMTASPV  KG     +    I
Sbjct: 164  SLLIFDECHRA--CGSHPYARIMKEFYFGS-QWRPDVFGMTASPVATKGASTLHNCEAHI 223

Query: 291  NSLEKLLDAKVYSVEDREDLEAF-VSSPLVKIYY--YGPVANGSSSSFMTYCCQLEEVKR 350
            + LE  LDAK+Y VEDR +LE+F   + +V  YY  Y    +   S    +  + + +  
Sbjct: 224  SQLELTLDAKIYIVEDRNELESFSPPTTIVNKYYDAYMVDFDNLKSKLQIFSDEFDSLLV 283

Query: 351  QCVVALGQMKGEHEGLLAT-KKLLLRMHDNIIFCLENLGVWGALQASKILLSGDN--SER 410
                +      + + +L T +K L R H  I++ L +LG     +  KI +       + 
Sbjct: 284  GLQESPSNKFKDTDNILETSRKSLSRYHGKILYSLNDLGPIITSEVVKIHIESVKPLCDS 343

Query: 411  SELIEAAEGNPNNDSLSDRYLNQAAEILASG----CKIDGGMPDMLNVDILEDPFFSKKL 470
             + I +      + S     L+   EIL  G     K + G     + ++ +  + S K+
Sbjct: 344  EDCIFSKASLCLHMSYFKEALSLIEEILPQGYGELMKSESG-----SEELTKRGYISSKV 403

Query: 471  LRLIGILSSFGHRLNMKCIIFVNRIVIARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMS 530
              LI I  SFG    + C+IFV+RI+ A+++   ++ +   + +   +L G  +   ++S
Sbjct: 404  NTLINIFKSFGSSNEVLCLIFVDRIITAKAVERFMRGIVNFSCFSISYLTGGSTSKDALS 463

Query: 531  RKTMNRILTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQ 590
                   L  FR+G++NLL  T V EEG+D+  C  VIRFDLP TV S++QSRGRAR   
Sbjct: 464  PAVQRFTLDLFRAGKVNLLFTTDVTEEGVDVPNCSCVIRFDLPRTVCSYVQSRGRARRNN 523

Query: 591  SEYAFIVDRGNEKE-----RGLIDGFQKDENHMNREISSRSSNETFQSHEESIYRVVSTG 650
            SE+  +++RGN ++     R +  G+      + R  ++ S + + Q      Y+V STG
Sbjct: 524  SEFILMIERGNLQQQEHIFRMIQTGYYVKNCALYRHPNALSYDLSIQG--MYTYQVQSTG 583

Query: 651  ASVTSGLSISLLHEYCSKLPHDEYFDPKPKFFYYDDLEGTICHAILPSNAPIPQVVSRVQ 710
            A++T+   ++L+ +YC KLP D YF PKP F    +     C   LP NA    +V  + 
Sbjct: 584  ATITADCCVNLIRKYCEKLPKDRYFMPKPSFEVTIEDGLFKCTLTLPRNAAFQSIVGPLS 643

Query: 711  FSRDAAKKDACLKAIEELHKLGAFNDFLLPMRDNAIEEELELASCDSDSSEDETSRRELH 770
             S + +K+   L+A ++LH+LG  ND L+P+ +  ++ +   A     S    T R+ELH
Sbjct: 644  SSSNLSKQLVSLEACKKLHQLGELNDHLVPLTEEPMDTDFTTADEKCISGPGTTKRKELH 703

Query: 771  EMLVPTALKDSWT-GSGNVILNCYQIKISPHPGDRIYKEFGLFVKAPLPQEAERIDLELH 830
                  AL  +W   S N+ LN Y+I          Y  F L ++  L  +     ++L 
Sbjct: 704  GTTCVLALSGTWIHDSENITLNTYRIDFLCDQEGENYAGFVLLMEPELDDDVAPSKMDLF 763

Query: 831  LARSRYVKVNLVPSGVVELLEDEITQAESFQEMFLKVILDR-------SEFVQDYVPLRN 890
            L  ++ V   + P G V+L + ++ + + FQE F   I  R       S   +D++  + 
Sbjct: 764  LIPNKMVYTTVTPRGKVQLNKKQLGKGKLFQEFFFNGIFGRLFHGSRKSGAQRDFIFKKG 823

Query: 891  NVSGSISSASYLLLPMIFQDNEGSSL-IDWKVIRRCLSS--QIFQNHA---------CSA 950
            +     + + YLLLP+         L I W+ I  C  +  Q++ ++          C  
Sbjct: 824  HEIQWNTESMYLLLPLRDSSYIQDDLSIHWEAIESCAGAVEQLWSSYQGDENVIPVNCIP 883

Query: 951  VRETASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSRY------KN 1010
             +     +  + L +       I++S+V   +    + V  ++        +      K 
Sbjct: 884  QKRRGGQEEIIHLANKSLHCSSIKDSVVLSLHTGRIYTVLDLILDTTAEDSFDEMCKGKA 943

Query: 1011 SGSLSHFEHLLERFDIHLKYPEQPLLCAKPLFHLHNLLHD--RRLNDSEACDLIEHLIDL 1070
            S   S  ++  +++ I +++PEQPLL  K   + HNLL    + L+ S    L+     +
Sbjct: 944  SPFTSFVDYYHQKYGIIIQHPEQPLLLLKQSHNAHNLLFSKLKYLDGSTGKPLLMEKEQI 1003

Query: 1071 ----PPELCQLKITGFTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSN 1130
                PPEL  L     T DI  S  LLPS +H L++ ++A +L+  +         +   
Sbjct: 1004 HARVPPEL--LIHLDVTTDILKSFYLLPSVIHRLQSLMLASQLRREIG----YNQHIPVT 1063

Query: 1131 RILEALTTEKCQERISLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNIN 1190
             ILEA+TT +C E  SLERLE+LGD+ LK+ V   LFL +    EG+L+  RS  V N  
Sbjct: 1064 LILEAITTLRCCETFSLERLELLGDSVLKYVVGCDLFLRYPMKHEGQLSDMRSKAVCNAT 1123

Query: 1191 LFKLATRKNLQVYIRDQPFEPNQFYSLG----RPCPRICNEET------RKDIHSHKNAK 1250
            L K    ++LQ Y+RD  F+P ++ + G    RP P  C  ET      R+ I    +  
Sbjct: 1124 LHKHGIWRSLQGYVRDNAFDPRRWVAPGQISLRPFPCNCGIETAFVPSHRRYIRDDPSFF 1183

Query: 1251 GGETKCSKGHHWLHKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDAR 1310
             G+  C +GH W+  KTISD VEALVGA+ V  G  AA+  ++W GI ++ +   + + +
Sbjct: 1184 VGK-PCDRGHRWMCSKTISDCVEALVGAYYVGGGIAAALWVMRWFGIDIKCDMKLLQEVK 1243

Query: 1311 MASNAYLRLADCISISALQDSLGHQFLYKGLLLQALIHPSYHRHG-GGCYQRLEFLGDAV 1370
              ++    L+    I  L+  L + F  KGLLL+A+ HPS    G   CYQRLEFLGD+V
Sbjct: 1244 FNASHLCSLSKINDIEELEAKLKYNFSVKGLLLEAITHPSLQELGVDYCYQRLEFLGDSV 1303

Query: 1371 LDYLITSYLYSVYPKLKPGLVTDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKS 1430
            LD L+T +LY+ +  + PG +TDLRS  V NE FA   V    +  L   S  L + I  
Sbjct: 1304 LDLLLTRHLYATHTDVDPGELTDLRSALVSNENFAQAVVRNNIHSHLQHGSGILLEQITE 1363

Query: 1431 YV-NFIKSPPSERGSLEQPTC--PKVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSI 1490
            YV + ++    E   L+  TC  PKVLGD++ESI GAV +DT F+++ VW+I    L  +
Sbjct: 1364 YVRSNLECQGKESEFLQHTTCKVPKVLGDIMESIAGAVFIDTDFNVDMVWEIFEPLLSPL 1423

Query: 1491 MSFSGFQLSPIRDITEFCQDCGWKLQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKD 1550
            ++     L P R++ E C   G  L    T   +   ++  ++  +    A   +R KK 
Sbjct: 1424 ITPDKLALPPYRELLELCSHIGCFLNSKCTSKGEEVIIEMSLQLRDELLVAQGHDRNKKR 1483

Query: 1551 AEKIAANLILSKLKEKGFIPEVSSLEEILRSRKKME--PELIGFDETPSVSIDQADNGSG 1610
            A+  AA+ IL+ LK++G      S+++ L   K+++     + FD T             
Sbjct: 1484 AKAKAASRILADLKQQGL-----SIKQCLSKAKQLDIVTSDLQFDLT------------- 1543

Query: 1611 TIPGSEHSGAELDPCVNSEFSNSHSVRITRLSEAPEFSSVAAEEELKPSTFGGTNLQSSS 1670
                   SG +L     S+ ++ H +         E  S   +E + P            
Sbjct: 1544 ------SSGTQLS---YSDLNDYHIL---------EGLSSVKKEVVLPL----------- 1603

Query: 1671 GRSSKTTARSRLYEICAANHWNQPSFD----------CMNEEGPGHLKMFTYKVVLEIEE 1688
             +  K   RS L+++C    W  P F+           M+     +   F   + L I +
Sbjct: 1604 -KMEKGGPRSALFKLCKILQWPMPEFEFVEQRFRTPIVMDGATTTNFNSFVSTITLHIPD 1629

BLAST of Sed0002244 vs. ExPASy TrEMBL
Match: A0A6J1C050 (dicer-like protein 4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007071 PE=3 SV=1)

HSP 1 Score: 2687.1 bits (6964), Expect = 0.0e+00
Identity = 1369/1640 (83.48%), Postives = 1471/1640 (89.70%), Query Frame = 0

Query: 68   DNSKPSSLA-VEKLMGLSLVGDPGAGSSSRASEKDPRRVARKYQLELCKKALEENIIVYL 127
            DN + +SL  VE  +GLSLV D   G SSRASEKDPRR ARKYQLELCKKALEENII+YL
Sbjct: 15   DNFQHTSLGPVESFVGLSLVDDQEPGPSSRASEKDPRRKARKYQLELCKKALEENIILYL 74

Query: 128  GTGCGKTHIAILLIYEMRNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVYCGG 187
            GTGCGKTHIA+LL+YEMR+LIRRSQKGICVFLAPTVALVQQQAKVIEDS DFKVRVYCGG
Sbjct: 75   GTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIEDSTDFKVRVYCGG 134

Query: 188  FKGLNSHYDWEREMEEHEVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSDHSY 247
             K LNSHYDWEREMEEHEVFVMTP+ LLR+L+HCYIKMEFVELLIFDECHHAQVKSDHSY
Sbjct: 135  SKCLNSHYDWEREMEEHEVFVMTPEILLRTLHHCYIKMEFVELLIFDECHHAQVKSDHSY 194

Query: 248  AQIMKVFYKGSVAKRPRIFGMTASPVVGKGTCNQQSLSKSINSLEKLLDAKVYSVEDRED 307
            A+IM+VFYK +VAKRPRIFGMTASPVVGKG CNQQ+LSKSINSLEKLLDAKVYSVEDRE+
Sbjct: 195  AEIMRVFYKTNVAKRPRIFGMTASPVVGKGACNQQNLSKSINSLEKLLDAKVYSVEDREE 254

Query: 308  LEAFVSSPLVKIYYYGPVANGSSSSFMTYCCQLEEVKRQCVVALGQMKGEHEGLLATKKL 367
            L+AFVSSPLVKIYYYGPVANG S SFMTYC  LE++KRQC+V LGQMKGEHE L ATKKL
Sbjct: 255  LDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGEHEDLRATKKL 314

Query: 368  LLRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNDSLSDRYLNQAA 427
            L RMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNN SLSDRYLNQAA
Sbjct: 315  LCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHSLSDRYLNQAA 374

Query: 428  EILASGCKIDGGMPDMLNVDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIVIAR 487
            EILA GCKI GGM D LN DILE PFFSKKLL+LI ILSSF HRLNMKCIIFVNRIVIAR
Sbjct: 375  EILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIAR 434

Query: 488  SLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGEEGL 547
            SLSYILQNL+FLA+WKCD+LVGVHSKLRSMSRKTMN IL KFRSGELNLL+ATKVGEEGL
Sbjct: 435  SLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGL 494

Query: 548  DIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDENHMN 607
            DIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAF+VD GNEKE GLID FQKDEN M+
Sbjct: 495  DIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMD 554

Query: 608  REISSRSSNETFQSHEESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKFFYY 667
            REISSRSS+E F SHEESIYRV STGAS+TSG SISLLHE+CSKLPHDEYFDPKPKFFYY
Sbjct: 555  REISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYY 614

Query: 668  DDLEGTICHAILPSNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPMRDN 727
            DDL GT+CHAILPSNAPI Q++SR Q SR+ AKKDAC+KAIEELHKLGA NDFLLPMRD+
Sbjct: 615  DDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLPMRDD 674

Query: 728  AIEEELELASCDSDSSEDETSRRELHEMLVPTALKDSWTGSGNVILNCYQIKISPHPGDR 787
            A EEELEL S DSD SEDETSR ELHEMLVP ALKDSWTGSG  ILNCYQIK SPHP DR
Sbjct: 675  ANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQIKFSPHPRDR 734

Query: 788  IYKEFGLFVKAPLPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQEMFL 847
            IYKEFGLFVKA LPQEAER+D+ELHLAR R V VNLVPSGVVELLEDEITQAESFQEMFL
Sbjct: 735  IYKEFGLFVKAALPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFL 794

Query: 848  KVILDRSEFVQDYVPLRNNVSGSISSASYLLLPMIFQDNEGSSLIDWKVIRRCLSSQIFQ 907
            KVILDRSEFVQ+++PLRNNVS SISS SYLLLP+IF DNE S  IDWKVIRRCL S IFQ
Sbjct: 795  KVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVIRRCLFSPIFQ 854

Query: 908  NHACSAVRETASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSR--Y 967
                S V+ETA S  HLTLYDGRRR  DI+NSLVYVPYKREFFFVT IV+ KNGYSR  Y
Sbjct: 855  IPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKGKNGYSRSHY 914

Query: 968  KNSGSLSHFEHLLERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLIDL 1027
            KNS S SHFEHL+ERF IHLKYPEQPLL AKPLFHLHNLLHDRR  +SEAC L E+ IDL
Sbjct: 915  KNSSSSSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEACYLQEYFIDL 974

Query: 1028 PPELCQLKITGFTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRILE 1087
            PPELCQLKI GF+KDIGSSISLLPS MH LEN LVAIELKC LAAAFP  +EVT+NRILE
Sbjct: 975  PPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGAEVTANRILE 1034

Query: 1088 ALTTEKCQERISLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFKL 1147
            ALTTEKCQERISLERLEILGD FLKFAVAR+LFLTHDK DEGELTRRRSYLVKNINLFKL
Sbjct: 1035 ALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYLVKNINLFKL 1094

Query: 1148 ATRKNLQVYIRDQPFEPNQFYSLGRPCPRICNEETRKDIHSH----KNAKGGETKCSKGH 1207
            ATRKNLQVYIRDQPFEP+QFY+LGRPCPRICNEETR+DIHSH    KNA+  ETKCS+GH
Sbjct: 1095 ATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNAQASETKCSRGH 1154

Query: 1208 HWLHKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRLA 1267
            HWLHKKTISDVVEALVGAFLVDSGFKAAI+FLKWIGIQVEFEAS VT+A M+SNAY+ LA
Sbjct: 1155 HWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALMSSNAYIILA 1214

Query: 1268 DCISISALQDSLGHQFLYKGLLLQALIHPSYHRHGGGCYQRLEFLGDAVLDYLITSYLYS 1327
            D I+IS LQ+SLGHQFL+KGLLLQAL+HPSYH+HGGGCYQRLEFLGDAVLDYLITSYLYS
Sbjct: 1215 DRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLDYLITSYLYS 1274

Query: 1328 VYPKLKPGLVTDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIKSPPSE 1387
             YPKLKPGL+TDLRSVFV NEAFAN++VDR FYK+LICDSTSLQ DIKSYVNFIK+P S 
Sbjct: 1275 AYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYVNFIKAPSSG 1334

Query: 1388 RGSLEQPTCPKVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRDI 1447
             GSL  P+CPK LGD+VES VGAVLVDTGFDMNYVWKIMLSFLD I+SFSGFQLSPIRDI
Sbjct: 1335 SGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSGFQLSPIRDI 1394

Query: 1448 TEFCQDCGWKLQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKLK 1507
             EFCQDCG  LQFN +K  KY+SV+A+VKG+NFH TASAA+RRK+DA+KIAANLIL+KLK
Sbjct: 1395 IEFCQDCGSNLQFNESKTGKYYSVEADVKGDNFHATASAAHRRKRDAKKIAANLILTKLK 1454

Query: 1508 EKGFIPEVSSLEEILRSRKKMEPELIGFDETPSVSIDQADNGSGTIPGSEHSGAELDPCV 1567
             +G IPEV+SLEEIL+S +KMEP+LIG+DETPSV++DQADNG GTI G E SGA+LDP V
Sbjct: 1455 AQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTMDQADNGFGTIKGLESSGADLDPFV 1514

Query: 1568 NSEFSNSHSVRITRLSEAPEFSSVAAEEELKPS-TFGG----TNLQSSSGRSSKTTARSR 1627
            +SE  N+H  RITR+S+    SS AA+E+L PS  F G    T++QSSSGR  K TARSR
Sbjct: 1515 HSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDGHDSPTDVQSSSGRLCKATARSR 1574

Query: 1628 LYEICAANHWNQPSFDCMNEEGPGHLKMFTYKVVLEIEEAPDTIFEFFGASHSRKKAAAE 1687
            LYEICAANHW+ PSFDCMNEEGP HLKMFTYKVVLEIEEAPDTIF+FFGA H RKKAAAE
Sbjct: 1575 LYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPDTIFKFFGAPHLRKKAAAE 1634

Query: 1688 HAAEAALWYLKKEGYWNETD 1696
            HAAEAA+WYLKKEGY  ETD
Sbjct: 1635 HAAEAAIWYLKKEGYLPETD 1653

BLAST of Sed0002244 vs. ExPASy TrEMBL
Match: A0A6J1JAJ5 (dicer-like protein 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485046 PE=3 SV=1)

HSP 1 Score: 2649.0 bits (6865), Expect = 0.0e+00
Identity = 1353/1655 (81.75%), Postives = 1455/1655 (87.92%), Query Frame = 0

Query: 64   SDFIDNSKPSSLAVEKLMGLSLVGDPGAGSSSRASEKDPRRVARKYQLELCKKALEENII 123
            +D ++     S +VE+ MGLS V D G GSS R S KDPRR+ARKYQLELCKKALEENII
Sbjct: 11   ADSVNFQPTISASVERFMGLSPVDDQGEGSSIRTSGKDPRRIARKYQLELCKKALEENII 70

Query: 124  VYLGTGCGKTHIAILLIYEMRNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVY 183
            VYLGTGCGKTHIAILLIYEM +LIRR QKGICVFLAPTVALVQQQAKVIEDSIDFKV+VY
Sbjct: 71   VYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIEDSIDFKVKVY 130

Query: 184  CGGFKGLNSHYDWEREMEEHEVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSD 243
            CGG K LNSHYDWEREMEE+EVFVMTP+ LLR+L+HCYIKMEFVELLIFDECHHAQVKSD
Sbjct: 131  CGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIKMEFVELLIFDECHHAQVKSD 190

Query: 244  HSYAQIMKVFYKGSVAKRPRIFGMTASPVVGKGTCNQQSLSKSINSLEKLLDAKVYSVED 303
            HSYA+IM+VFYK +  KRPRIFGMTASPVVGKG CNQQ+LSKSINSLEKLLDAKVYSVED
Sbjct: 191  HSYAEIMRVFYKANEHKRPRIFGMTASPVVGKGACNQQNLSKSINSLEKLLDAKVYSVED 250

Query: 304  REDLEAFVSSPLVKIYYYGPVANGSSSSFMTYCCQLEEVKRQCVVALGQMKGEHEGLLAT 363
            RE+LEAFVSSP  KIYYYGPVA GSSSSFMTYCC LE+VK QC+VALGQMKGEHEGLLAT
Sbjct: 251  REELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKCQCIVALGQMKGEHEGLLAT 310

Query: 364  KKLLLRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNDSLSDRYLN 423
            KKLL RMH NIIFCLENLGVWGALQASKILLS DNSERSELIEAAEGNP  DS+SDRYLN
Sbjct: 311  KKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSELIEAAEGNPKYDSISDRYLN 370

Query: 424  QAAEILASGCKIDGGMPDMLNVDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIV 483
            QAAEI ASGCKID G+ DMLNVDIL++P FSKKLLRLIGILSSF HR NMKCIIFVNRIV
Sbjct: 371  QAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGILSSFRHRPNMKCIIFVNRIV 430

Query: 484  IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGE 543
            IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMN ILTKFRSGELNLLIATKVGE
Sbjct: 431  IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHILTKFRSGELNLLIATKVGE 490

Query: 544  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDEN 603
            EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAF+VD GNEK+ GLID F KDE 
Sbjct: 491  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKDLGLIDEFHKDEK 550

Query: 604  HMNREISSRSSNETFQSHEESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKF 663
             MNREISSRSS ETF  HEESIYRVVSTGASVTSG SISLLHEYCSKLPHD YFDPKPKF
Sbjct: 551  RMNREISSRSSIETFDGHEESIYRVVSTGASVTSGRSISLLHEYCSKLPHDTYFDPKPKF 610

Query: 664  FYYDDLEGTICHAILPSNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPM 723
            FY DDL GTICHAILPSNAPIPQ+VSR Q S  AAKKDACLKA+EELHKLGA +D+LLPM
Sbjct: 611  FYCDDLGGTICHAILPSNAPIPQIVSRPQPSGVAAKKDACLKAVEELHKLGALSDYLLPM 670

Query: 724  RDNAIEEELELASCDSDSSEDETSRRELHEMLVPTALKDSWTGSGNVILNCYQIKISPHP 783
            RDNA E ELEL S DSDSSEDETSR ELHEM+ P A K+SWTG G VIL CY IK +P+P
Sbjct: 671  RDNANEGELELDS-DSDSSEDETSRLELHEMISPAAFKESWTGFGYVILYCYHIKFTPNP 730

Query: 784  GDRIYKEFGLFVKAPLPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQE 843
             DRIYKEFGLFVKAPLPQEA R+DLELHLA  R VKVNL+PS VVELLE+EITQAE FQE
Sbjct: 731  EDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMPSRVVELLENEITQAECFQE 790

Query: 844  MFLKVILDRSEFVQDYVPLRNNVSGSISSASYLLLPMIFQDNEGSSLIDWKVIRRCLSSQ 903
            MFLKVILDRSEFV DY+PLRNNVS SISS SYLLLP+I  DNEGS  IDWKVIRRCLSSQ
Sbjct: 791  MFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHDNEGSVFIDWKVIRRCLSSQ 850

Query: 904  IFQNHACSAVRETASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSR 963
            IFQNHACS ++ETASSDTHL LYDGRRR  DIENSLVYVPYKREFFFVT+IVR KNGYS+
Sbjct: 851  IFQNHACSIIKETASSDTHLMLYDGRRRSSDIENSLVYVPYKREFFFVTNIVRRKNGYSQ 910

Query: 964  YKNSGSLSHFEHLLERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLID 1023
            YK+SGSLSHFEHL  RF IHLKYPEQPLLCAKPLF LHNLLH+R+  DSEA  L E+ ID
Sbjct: 911  YKDSGSLSHFEHLKTRFGIHLKYPEQPLLCAKPLFSLHNLLHNRKQKDSEAPYLEEYFID 970

Query: 1024 LPPELCQLKITGFTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRIL 1083
            + PELCQLKITGF+KDIGSSISLLPS MH LEN LVAIELKC LAAAFPA +EVT+NRIL
Sbjct: 971  VAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPAGAEVTANRIL 1030

Query: 1084 EALTTEKCQERISLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFK 1143
            EALTTEKCQER SLERLEILGD+FLKFAVAR+LFL HDKFDEGELTRRRSYLVKNINLFK
Sbjct: 1031 EALTTEKCQERFSLERLEILGDSFLKFAVARYLFLAHDKFDEGELTRRRSYLVKNINLFK 1090

Query: 1144 LATRKNLQVYIRDQPFEPNQFYSLGRPCPRICNEETRKDIHSH----KNAKGGETKCSKG 1203
            LATRKNLQVYIRDQPFEP+QFY LGRPCPRICNEETR DIHSH     NAK  ETKCSKG
Sbjct: 1091 LATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRNDIHSHDNATNNAKFSETKCSKG 1150

Query: 1204 HHWLHKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRL 1263
            HHWL KKTISD+VEAL+GAFLVDSGFKAAIAFLKWIGI+VEFEASQVT+A +AS AY+  
Sbjct: 1151 HHWLQKKTISDMVEALIGAFLVDSGFKAAIAFLKWIGIKVEFEASQVTNALVASKAYVLF 1210

Query: 1264 ADCISISALQDSLGHQFLYKGLLLQALIHPSYHRHGGGCYQRLEFLGDAVLDYLITSYLY 1323
            AD I+ISALQ SLGH+FL+KGLLLQAL+HP+YH+HGGGCYQRLEFLGDAVLDYLI SYLY
Sbjct: 1211 ADTINISALQSSLGHKFLHKGLLLQALVHPTYHKHGGGCYQRLEFLGDAVLDYLIMSYLY 1270

Query: 1324 SVYPKLKPGLVTDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIKSPPS 1383
            SVYPKLKPGL+TDLRSVFV NEAFAN++VDRFFYKFL+CDS SL+ DIKSYV+FIK+PPS
Sbjct: 1271 SVYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLCDSASLESDIKSYVDFIKAPPS 1330

Query: 1384 ERGSLEQPTCPKVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRD 1443
            ER SLEQP CPK LGD+VES VGAVLVDTGFDMNYVWKIMLSFLD IMS SGFQLSPIRD
Sbjct: 1331 ERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKIMLSFLDPIMSSSGFQLSPIRD 1390

Query: 1444 ITEFCQDCGWKLQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKL 1503
            I E CQ+CGWKLQFN +K+EKY+SV+AEVKG++FH TASA NRRKKDAEKIAAN IL KL
Sbjct: 1391 IIEVCQNCGWKLQFNPSKMEKYYSVEAEVKGDDFHATASAVNRRKKDAEKIAANAILRKL 1450

Query: 1504 KEKGFIPEVSSLEEILRSRKKMEPELIGFDETPSVSIDQADNGSGTIPGSEHSGAELDPC 1563
            K +GFIPEV+SLE+IL+S +KM+P+LIG+DETPSV++D+ D+      G E SGA+LDP 
Sbjct: 1451 KAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDRIDD------GLEFSGADLDPL 1510

Query: 1564 VNSEFSNSHSVRITRLSEAPEFSSVAAEEELKP-------------------STFGGTNL 1623
            V+ E  NS   RITR+SE    SS AA E+LKP                   S+   ++ 
Sbjct: 1511 VHREVGNSRPFRITRMSETLASSSGAAGEQLKPSIPLEGHDSSTDLLSSSSSSSSSSSSS 1570

Query: 1624 QSSSGRSSKTTARSRLYEICAANHWNQPSFDCMNEEGPGHLKMFTYKVVLEIEEAPDTIF 1683
             SSSGRS KTTARSRLYE+CA NHWN PSF+CMNEEGP HLKMFTYKVVLEIEEAPDTIF
Sbjct: 1571 SSSSGRSGKTTARSRLYEVCAINHWNHPSFNCMNEEGPSHLKMFTYKVVLEIEEAPDTIF 1630

Query: 1684 EFFGASHSRKKAAAEHAAEAALWYLKKEGYWNETD 1696
            EFFGA H  KKAAAEHAAEAALWYLKKE Y  +TD
Sbjct: 1631 EFFGAPHLTKKAAAEHAAEAALWYLKKERYLGDTD 1658

BLAST of Sed0002244 vs. ExPASy TrEMBL
Match: A0A6J1E1P4 (dicer-like protein 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429985 PE=3 SV=1)

HSP 1 Score: 2648.6 bits (6864), Expect = 0.0e+00
Identity = 1354/1651 (82.01%), Postives = 1456/1651 (88.19%), Query Frame = 0

Query: 64   SDFIDNSKPSSLAVEKLMGLSLVGDPGAGSSSRASEKDPRRVARKYQLELCKKALEENII 123
            +D ++    SS +VE+ MGLS V D GAGSS R S KDPRR+ARKYQLELC+KALEENII
Sbjct: 11   ADSVNFQPTSSASVERFMGLSPVDDQGAGSSIRTSGKDPRRIARKYQLELCEKALEENII 70

Query: 124  VYLGTGCGKTHIAILLIYEMRNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVY 183
            VYLGTGCGKTHIAILLIYEM +LIRR QKGICVFLAPTVALVQQQAKVIEDSIDFKV+VY
Sbjct: 71   VYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIEDSIDFKVKVY 130

Query: 184  CGGFKGLNSHYDWEREMEEHEVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSD 243
            CGG K LNSHYDWEREMEE+EVFVMTP+ LLR+L+HCYI+MEFVELLIFDECHHAQVKSD
Sbjct: 131  CGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIRMEFVELLIFDECHHAQVKSD 190

Query: 244  HSYAQIMKVFYKGSVAKRPRIFGMTASPVVGKGTCNQQSLSKSINSLEKLLDAKVYSVED 303
            HSYA+IM+VFYK +  KRPRIFGMTASPVVGKG CNQQ+LSKSINSLEKLLDAKVYSVED
Sbjct: 191  HSYAEIMRVFYKANEHKRPRIFGMTASPVVGKGACNQQNLSKSINSLEKLLDAKVYSVED 250

Query: 304  REDLEAFVSSPLVKIYYYGPVANGSSSSFMTYCCQLEEVKRQCVVALGQMKGEHEGLLAT 363
            RE+LEAFVSSP  KIYYYGPVA GSSSSFMTYCC LE+VK QC+VALGQMKGEHEGLLAT
Sbjct: 251  REELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKCQCIVALGQMKGEHEGLLAT 310

Query: 364  KKLLLRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNDSLSDRYLN 423
            KKLL RMH NIIFCLENLGVWGALQASKILLS DNSERSELIEAA  NP +DS+SDRYLN
Sbjct: 311  KKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSELIEAAAANPKHDSISDRYLN 370

Query: 424  QAAEILASGCKIDGGMPDMLNVDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIV 483
            QAAEI ASGCKID G+ DMLNVDIL++P FSKKLLRLIGILSSF HR NMKCIIFVNRIV
Sbjct: 371  QAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGILSSFRHRPNMKCIIFVNRIV 430

Query: 484  IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGE 543
            IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMN ILTKFRSGELNLLIATKVGE
Sbjct: 431  IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHILTKFRSGELNLLIATKVGE 490

Query: 544  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDEN 603
            EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAF+VD GNEKE GLID F KDE 
Sbjct: 491  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFHKDEK 550

Query: 604  HMNREISSRSSNETFQSHEESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKF 663
             MNREISSRSS ETF  HEESIYRV+STGASVTSG SISLLHEYCSKLPHD YFDPKPKF
Sbjct: 551  RMNREISSRSSVETFDGHEESIYRVISTGASVTSGRSISLLHEYCSKLPHDTYFDPKPKF 610

Query: 664  FYYDDLEGTICHAILPSNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPM 723
            FY DDL GTICHAILPSNAPIPQ+VSR Q S  AAKKDACLKA+EELHKLGA +D+LLPM
Sbjct: 611  FYCDDLGGTICHAILPSNAPIPQIVSRPQPSGVAAKKDACLKAVEELHKLGALSDYLLPM 670

Query: 724  RDNAIEEELELASCDSDSSEDETSRRELHEMLVPTALKDSWTGSGNVILNCYQIKISPHP 783
            RDNA E ELEL S DSDSSEDETSRRELHEM+ P A K+SWTG G VIL CY IK +P+P
Sbjct: 671  RDNANEGELELDS-DSDSSEDETSRRELHEMISPAAFKESWTGFGYVILYCYHIKFTPNP 730

Query: 784  GDRIYKEFGLFVKAPLPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQE 843
             DRIYKEFGLFVKAPLPQEA R+DLELHLA  R VKVNL+PS VVEL+E+EITQAE FQE
Sbjct: 731  EDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMPSRVVELMENEITQAECFQE 790

Query: 844  MFLKVILDRSEFVQDYVPLRNNVSGSISSASYLLLPMIFQDNEGSSLIDWKVIRRCLSSQ 903
            MFLKVILDRSEFV DY+PLRNNVS SISS SYLLLP+I  D EGS  IDWKVIRRCLSSQ
Sbjct: 791  MFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHDIEGSVFIDWKVIRRCLSSQ 850

Query: 904  IFQNHACSAVRETASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSR 963
            IFQNHACS V+ETASSDTHL LYDGRRR  DIENSLVYVPYKREFFFVT+IVR KNGYS+
Sbjct: 851  IFQNHACSIVKETASSDTHLILYDGRRRSSDIENSLVYVPYKREFFFVTNIVRRKNGYSQ 910

Query: 964  YKNSGSLSHFEHLLERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLID 1023
            YK+SGSLSHFEHL  RF I LKYPEQPLLCAKPLF LHNLLHDR+  DSEA  L E+ ID
Sbjct: 911  YKDSGSLSHFEHLKTRFGIQLKYPEQPLLCAKPLFSLHNLLHDRKQKDSEAPYLEEYFID 970

Query: 1024 LPPELCQLKITGFTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRIL 1083
            + PELCQLKITGF+KDIGSSISLLPS MH LEN LVAIELKC LAA FPA +EVT+NRIL
Sbjct: 971  IAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAGFPAGAEVTANRIL 1030

Query: 1084 EALTTEKCQERISLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFK 1143
            EALTTEKCQERISLERLEILGD+FLKFAVAR+LFL HDKFDEGELTRRRSYLVKNINLFK
Sbjct: 1031 EALTTEKCQERISLERLEILGDSFLKFAVARYLFLAHDKFDEGELTRRRSYLVKNINLFK 1090

Query: 1144 LATRKNLQVYIRDQPFEPNQFYSLGRPCPRICNEETRKDIHSH----KNAKGGETKCSKG 1203
            LATRKNLQVYIRDQPFEP+QFY LGRPCPRICNEETR DIHSH     NAK  ETKCSKG
Sbjct: 1091 LATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRNDIHSHDNATNNAKASETKCSKG 1150

Query: 1204 HHWLHKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRL 1263
            HHWL KKTISDVVEALVGAFLVDSGFKAAIAFLKWIGI+VEFEASQVT+A +AS AY+ L
Sbjct: 1151 HHWLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIKVEFEASQVTNALVASKAYVLL 1210

Query: 1264 ADCISISALQDSLGHQFLYKGLLLQALIHPSYHRHGGGCYQRLEFLGDAVLDYLITSYLY 1323
            AD I+ISALQ SLG++FL+KGLLLQAL+HP+YH+HGGGCYQRLEFLGDAVLDYLI SYLY
Sbjct: 1211 ADTINISALQSSLGYKFLHKGLLLQALVHPTYHKHGGGCYQRLEFLGDAVLDYLIMSYLY 1270

Query: 1324 SVYPKLKPGLVTDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIKSPPS 1383
            S YPKLKPGL+TDLRSVFV NEAFAN++VDRFFYKFL+CDS SLQ DIKSYV+FIKS PS
Sbjct: 1271 SAYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLCDSASLQSDIKSYVDFIKSLPS 1330

Query: 1384 ERGSLEQPTCPKVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRD 1443
            ER SLEQP CPK LGD+VES VGAVLVDTGFDMNYVWKIMLSFLD IMS SGFQLSPIRD
Sbjct: 1331 ERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKIMLSFLDPIMSSSGFQLSPIRD 1390

Query: 1444 ITEFCQDCGWKLQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKL 1503
            I E CQ+CGWKLQFN +K+EKY+SV+AEVKG++FH TASA NRRKKDAEKIAAN IL+KL
Sbjct: 1391 IIEVCQNCGWKLQFNPSKMEKYYSVEAEVKGDDFHATASAVNRRKKDAEKIAANSILTKL 1450

Query: 1504 KEKGFIPEVSSLEEILRSRKKMEPELIGFDETPSVSIDQADNGSGTIPGSEHSGAELDPC 1563
            K +GFIPEV+SLE+IL+S +KM+P+LIG+DETPSV++DQ D+      G E SGA+LDP 
Sbjct: 1451 KAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDQIDD------GLEFSGADLDPR 1510

Query: 1564 VNSEFSNSHSVRITRLSEAPEFSSVAAEEELKP---------------STFGGTNLQSSS 1623
            V+ E  NSH  RITR+SE    SS AA E+LKP               S+   ++  SSS
Sbjct: 1511 VHHEVGNSHPFRITRMSETLASSSGAAGEQLKPSIPLEGHDSSTDLLSSSSSSSSSSSSS 1570

Query: 1624 GRSSKTTARSRLYEICAANHWNQPSFDCMNEEGPGHLKMFTYKVVLEIEEAPDTIFEFFG 1683
            GRS KTTARSRL+E+CA NHWN PSF+CMNEEGP HLKMFTYKVVLEIEEAPDTIFEFFG
Sbjct: 1571 GRSGKTTARSRLHEVCAINHWNHPSFNCMNEEGPSHLKMFTYKVVLEIEEAPDTIFEFFG 1630

Query: 1684 ASHSRKKAAAEHAAEAALWYLKKEGYWNETD 1696
            A H  KKAAAEHAAEAALWYLKKE Y  +TD
Sbjct: 1631 APHLTKKAAAEHAAEAALWYLKKERYLGDTD 1654

BLAST of Sed0002244 vs. ExPASy TrEMBL
Match: A0A6J1JJM5 (dicer-like protein 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485046 PE=3 SV=1)

HSP 1 Score: 2642.5 bits (6848), Expect = 0.0e+00
Identity = 1352/1655 (81.69%), Postives = 1454/1655 (87.85%), Query Frame = 0

Query: 64   SDFIDNSKPSSLAVEKLMGLSLVGDPGAGSSSRASEKDPRRVARKYQLELCKKALEENII 123
            +D ++     S +VE+ MGLS V D G GSS R S KDPRR+ARKYQLELCKKALEENII
Sbjct: 11   ADSVNFQPTISASVERFMGLSPVDDQGEGSSIRTSGKDPRRIARKYQLELCKKALEENII 70

Query: 124  VYLGTGCGKTHIAILLIYEMRNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVY 183
            VYLGTGCGKTHIAILLIYEM +LIRR QKGICVFLAPTVALVQQQAKVIEDSIDFKV+VY
Sbjct: 71   VYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIEDSIDFKVKVY 130

Query: 184  CGGFKGLNSHYDWEREMEEHEVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSD 243
            CGG K LNSHYDWEREMEE+EVFVMTP+ LLR+L+HCYIKMEFVELLIFDECHHAQVKSD
Sbjct: 131  CGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIKMEFVELLIFDECHHAQVKSD 190

Query: 244  HSYAQIMKVFYKGSVAKRPRIFGMTASPVVGKGTCNQQSLSKSINSLEKLLDAKVYSVED 303
            HSYA+IM+VFYK +  KRPRIFGMTASPVVGKG CNQQ+LSKSINSLEKLLDAKVYSVED
Sbjct: 191  HSYAEIMRVFYKANEHKRPRIFGMTASPVVGKGACNQQNLSKSINSLEKLLDAKVYSVED 250

Query: 304  REDLEAFVSSPLVKIYYYGPVANGSSSSFMTYCCQLEEVKRQCVVALGQMKGEHEGLLAT 363
            RE+LEAFVSSP  KIYYYGPVA GSSSSFMTYCC LE+VK QC+VALGQMKGEHEGLLAT
Sbjct: 251  REELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKCQCIVALGQMKGEHEGLLAT 310

Query: 364  KKLLLRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNDSLSDRYLN 423
            KKLL RMH NIIFCLENLGVWGALQASKILLS DNSERSELIEAAEGNP  DS+SDRYLN
Sbjct: 311  KKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSELIEAAEGNPKYDSISDRYLN 370

Query: 424  QAAEILASGCKIDGGMPDMLNVDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIV 483
            QAAEI ASGCKID G+ DMLNVDIL++P FSKKLLRLIGILSSF HR NMKCIIFVNRIV
Sbjct: 371  QAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGILSSFRHRPNMKCIIFVNRIV 430

Query: 484  IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGE 543
            IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMN ILTKFRSGELNLLIATKVGE
Sbjct: 431  IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHILTKFRSGELNLLIATKVGE 490

Query: 544  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDEN 603
            EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAF+VD GNEK+ GLID F KDE 
Sbjct: 491  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKDLGLIDEFHKDEK 550

Query: 604  HMNREISSRSSNETFQSHEESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKF 663
             MNREISSRSS ETF  HEESIYRVVSTGASVTSG SISLLHEYCSKLPHD YFDPKPKF
Sbjct: 551  RMNREISSRSSIETFDGHEESIYRVVSTGASVTSGRSISLLHEYCSKLPHDTYFDPKPKF 610

Query: 664  FYYDDLEGTICHAILPSNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPM 723
            FY DDL GTICHAILPSNAPIPQ+VSR Q S  AAKKDACLKA+EELHKLGA +D+LLPM
Sbjct: 611  FYCDDLGGTICHAILPSNAPIPQIVSRPQPSGVAAKKDACLKAVEELHKLGALSDYLLPM 670

Query: 724  RDNAIEEELELASCDSDSSEDETSRRELHEMLVPTALKDSWTGSGNVILNCYQIKISPHP 783
            RDNA E ELEL S DSDSSEDETSR ELHEM+ P A K+SWTG G VIL CY IK +P+P
Sbjct: 671  RDNANEGELELDS-DSDSSEDETSRLELHEMISPAAFKESWTGFGYVILYCYHIKFTPNP 730

Query: 784  GDRIYKEFGLFVKAPLPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQE 843
             DRIYKEFGLFVKAPLPQEA R+DLELHLA  R VKVNL+PS VVELLE+EITQAE FQE
Sbjct: 731  EDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMPSRVVELLENEITQAECFQE 790

Query: 844  MFLKVILDRSEFVQDYVPLRNNVSGSISSASYLLLPMIFQDNEGSSLIDWKVIRRCLSSQ 903
            MFLKVILDRSEFV DY+PLRNNVS SISS SYLLLP+I  DNEGS  IDWKVIRRCLSSQ
Sbjct: 791  MFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHDNEGSVFIDWKVIRRCLSSQ 850

Query: 904  IFQNHACSAVRETASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSR 963
            IFQNHACS ++ETASSDTHL LYDGRRR  DIENSLVYVPYKREFFFVT+IVR KNGYS+
Sbjct: 851  IFQNHACSIIKETASSDTHLMLYDGRRRSSDIENSLVYVPYKREFFFVTNIVRRKNGYSQ 910

Query: 964  YKNSGSLSHFEHLLERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLID 1023
            YK+SGSLSHFEHL  RF IHLKYPEQPLLCAKPLF LHNLLH+R+  DS A  L E+ ID
Sbjct: 911  YKDSGSLSHFEHLKTRFGIHLKYPEQPLLCAKPLFSLHNLLHNRKQKDS-APYLEEYFID 970

Query: 1024 LPPELCQLKITGFTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRIL 1083
            + PELCQLKITGF+KDIGSSISLLPS MH LEN LVAIELKC LAAAFPA +EVT+NRIL
Sbjct: 971  VAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPAGAEVTANRIL 1030

Query: 1084 EALTTEKCQERISLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFK 1143
            EALTTEKCQER SLERLEILGD+FLKFAVAR+LFL HDKFDEGELTRRRSYLVKNINLFK
Sbjct: 1031 EALTTEKCQERFSLERLEILGDSFLKFAVARYLFLAHDKFDEGELTRRRSYLVKNINLFK 1090

Query: 1144 LATRKNLQVYIRDQPFEPNQFYSLGRPCPRICNEETRKDIHSH----KNAKGGETKCSKG 1203
            LATRKNLQVYIRDQPFEP+QFY LGRPCPRICNEETR DIHSH     NAK  ETKCSKG
Sbjct: 1091 LATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRNDIHSHDNATNNAKFSETKCSKG 1150

Query: 1204 HHWLHKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRL 1263
            HHWL KKTISD+VEAL+GAFLVDSGFKAAIAFLKWIGI+VEFEASQVT+A +AS AY+  
Sbjct: 1151 HHWLQKKTISDMVEALIGAFLVDSGFKAAIAFLKWIGIKVEFEASQVTNALVASKAYVLF 1210

Query: 1264 ADCISISALQDSLGHQFLYKGLLLQALIHPSYHRHGGGCYQRLEFLGDAVLDYLITSYLY 1323
            AD I+ISALQ SLGH+FL+KGLLLQAL+HP+YH+HGGGCYQRLEFLGDAVLDYLI SYLY
Sbjct: 1211 ADTINISALQSSLGHKFLHKGLLLQALVHPTYHKHGGGCYQRLEFLGDAVLDYLIMSYLY 1270

Query: 1324 SVYPKLKPGLVTDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIKSPPS 1383
            SVYPKLKPGL+TDLRSVFV NEAFAN++VDRFFYKFL+CDS SL+ DIKSYV+FIK+PPS
Sbjct: 1271 SVYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLCDSASLESDIKSYVDFIKAPPS 1330

Query: 1384 ERGSLEQPTCPKVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRD 1443
            ER SLEQP CPK LGD+VES VGAVLVDTGFDMNYVWKIMLSFLD IMS SGFQLSPIRD
Sbjct: 1331 ERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKIMLSFLDPIMSSSGFQLSPIRD 1390

Query: 1444 ITEFCQDCGWKLQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKL 1503
            I E CQ+CGWKLQFN +K+EKY+SV+AEVKG++FH TASA NRRKKDAEKIAAN IL KL
Sbjct: 1391 IIEVCQNCGWKLQFNPSKMEKYYSVEAEVKGDDFHATASAVNRRKKDAEKIAANAILRKL 1450

Query: 1504 KEKGFIPEVSSLEEILRSRKKMEPELIGFDETPSVSIDQADNGSGTIPGSEHSGAELDPC 1563
            K +GFIPEV+SLE+IL+S +KM+P+LIG+DETPSV++D+ D+      G E SGA+LDP 
Sbjct: 1451 KAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDRIDD------GLEFSGADLDPL 1510

Query: 1564 VNSEFSNSHSVRITRLSEAPEFSSVAAEEELKP-------------------STFGGTNL 1623
            V+ E  NS   RITR+SE    SS AA E+LKP                   S+   ++ 
Sbjct: 1511 VHREVGNSRPFRITRMSETLASSSGAAGEQLKPSIPLEGHDSSTDLLSSSSSSSSSSSSS 1570

Query: 1624 QSSSGRSSKTTARSRLYEICAANHWNQPSFDCMNEEGPGHLKMFTYKVVLEIEEAPDTIF 1683
             SSSGRS KTTARSRLYE+CA NHWN PSF+CMNEEGP HLKMFTYKVVLEIEEAPDTIF
Sbjct: 1571 SSSSGRSGKTTARSRLYEVCAINHWNHPSFNCMNEEGPSHLKMFTYKVVLEIEEAPDTIF 1630

Query: 1684 EFFGASHSRKKAAAEHAAEAALWYLKKEGYWNETD 1696
            EFFGA H  KKAAAEHAAEAALWYLKKE Y  +TD
Sbjct: 1631 EFFGAPHLTKKAAAEHAAEAALWYLKKERYLGDTD 1657

BLAST of Sed0002244 vs. ExPASy TrEMBL
Match: A0A6J1E2I1 (dicer-like protein 4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429985 PE=3 SV=1)

HSP 1 Score: 2642.1 bits (6847), Expect = 0.0e+00
Identity = 1353/1651 (81.95%), Postives = 1455/1651 (88.13%), Query Frame = 0

Query: 64   SDFIDNSKPSSLAVEKLMGLSLVGDPGAGSSSRASEKDPRRVARKYQLELCKKALEENII 123
            +D ++    SS +VE+ MGLS V D GAGSS R S KDPRR+ARKYQLELC+KALEENII
Sbjct: 11   ADSVNFQPTSSASVERFMGLSPVDDQGAGSSIRTSGKDPRRIARKYQLELCEKALEENII 70

Query: 124  VYLGTGCGKTHIAILLIYEMRNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVY 183
            VYLGTGCGKTHIAILLIYEM +LIRR QKGICVFLAPTVALVQQQAKVIEDSIDFKV+VY
Sbjct: 71   VYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIEDSIDFKVKVY 130

Query: 184  CGGFKGLNSHYDWEREMEEHEVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSD 243
            CGG K LNSHYDWEREMEE+EVFVMTP+ LLR+L+HCYI+MEFVELLIFDECHHAQVKSD
Sbjct: 131  CGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIRMEFVELLIFDECHHAQVKSD 190

Query: 244  HSYAQIMKVFYKGSVAKRPRIFGMTASPVVGKGTCNQQSLSKSINSLEKLLDAKVYSVED 303
            HSYA+IM+VFYK +  KRPRIFGMTASPVVGKG CNQQ+LSKSINSLEKLLDAKVYSVED
Sbjct: 191  HSYAEIMRVFYKANEHKRPRIFGMTASPVVGKGACNQQNLSKSINSLEKLLDAKVYSVED 250

Query: 304  REDLEAFVSSPLVKIYYYGPVANGSSSSFMTYCCQLEEVKRQCVVALGQMKGEHEGLLAT 363
            RE+LEAFVSSP  KIYYYGPVA GSSSSFMTYCC LE+VK QC+VALGQMKGEHEGLLAT
Sbjct: 251  REELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKCQCIVALGQMKGEHEGLLAT 310

Query: 364  KKLLLRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNDSLSDRYLN 423
            KKLL RMH NIIFCLENLGVWGALQASKILLS DNSERSELIEAA  NP +DS+SDRYLN
Sbjct: 311  KKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSELIEAAAANPKHDSISDRYLN 370

Query: 424  QAAEILASGCKIDGGMPDMLNVDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIV 483
            QAAEI ASGCKID G+ DMLNVDIL++P FSKKLLRLIGILSSF HR NMKCIIFVNRIV
Sbjct: 371  QAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGILSSFRHRPNMKCIIFVNRIV 430

Query: 484  IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGE 543
            IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMN ILTKFRSGELNLLIATKVGE
Sbjct: 431  IARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHILTKFRSGELNLLIATKVGE 490

Query: 544  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDEN 603
            EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAF+VD GNEKE GLID F KDE 
Sbjct: 491  EGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFHKDEK 550

Query: 604  HMNREISSRSSNETFQSHEESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKF 663
             MNREISSRSS ETF  HEESIYRV+STGASVTSG SISLLHEYCSKLPHD YFDPKPKF
Sbjct: 551  RMNREISSRSSVETFDGHEESIYRVISTGASVTSGRSISLLHEYCSKLPHDTYFDPKPKF 610

Query: 664  FYYDDLEGTICHAILPSNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPM 723
            FY DDL GTICHAILPSNAPIPQ+VSR Q S  AAKKDACLKA+EELHKLGA +D+LLPM
Sbjct: 611  FYCDDLGGTICHAILPSNAPIPQIVSRPQPSGVAAKKDACLKAVEELHKLGALSDYLLPM 670

Query: 724  RDNAIEEELELASCDSDSSEDETSRRELHEMLVPTALKDSWTGSGNVILNCYQIKISPHP 783
            RDNA E ELEL S DSDSSEDETSRRELHEM+ P A K+SWTG G VIL CY IK +P+P
Sbjct: 671  RDNANEGELELDS-DSDSSEDETSRRELHEMISPAAFKESWTGFGYVILYCYHIKFTPNP 730

Query: 784  GDRIYKEFGLFVKAPLPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQE 843
             DRIYKEFGLFVKAPLPQEA R+DLELHLA  R VKVNL+PS VVEL+E+EITQAE FQE
Sbjct: 731  EDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMPSRVVELMENEITQAECFQE 790

Query: 844  MFLKVILDRSEFVQDYVPLRNNVSGSISSASYLLLPMIFQDNEGSSLIDWKVIRRCLSSQ 903
            MFLKVILDRSEFV DY+PLRNNVS SISS SYLLLP+I  D EGS  IDWKVIRRCLSSQ
Sbjct: 791  MFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHDIEGSVFIDWKVIRRCLSSQ 850

Query: 904  IFQNHACSAVRETASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSR 963
            IFQNHACS V+ETASSDTHL LYDGRRR  DIENSLVYVPYKREFFFVT+IVR KNGYS+
Sbjct: 851  IFQNHACSIVKETASSDTHLILYDGRRRSSDIENSLVYVPYKREFFFVTNIVRRKNGYSQ 910

Query: 964  YKNSGSLSHFEHLLERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLID 1023
            YK+SGSLSHFEHL  RF I LKYPEQPLLCAKPLF LHNLLHDR+  DS A  L E+ ID
Sbjct: 911  YKDSGSLSHFEHLKTRFGIQLKYPEQPLLCAKPLFSLHNLLHDRKQKDS-APYLEEYFID 970

Query: 1024 LPPELCQLKITGFTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRIL 1083
            + PELCQLKITGF+KDIGSSISLLPS MH LEN LVAIELKC LAA FPA +EVT+NRIL
Sbjct: 971  IAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAGFPAGAEVTANRIL 1030

Query: 1084 EALTTEKCQERISLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFK 1143
            EALTTEKCQERISLERLEILGD+FLKFAVAR+LFL HDKFDEGELTRRRSYLVKNINLFK
Sbjct: 1031 EALTTEKCQERISLERLEILGDSFLKFAVARYLFLAHDKFDEGELTRRRSYLVKNINLFK 1090

Query: 1144 LATRKNLQVYIRDQPFEPNQFYSLGRPCPRICNEETRKDIHSH----KNAKGGETKCSKG 1203
            LATRKNLQVYIRDQPFEP+QFY LGRPCPRICNEETR DIHSH     NAK  ETKCSKG
Sbjct: 1091 LATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRNDIHSHDNATNNAKASETKCSKG 1150

Query: 1204 HHWLHKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRL 1263
            HHWL KKTISDVVEALVGAFLVDSGFKAAIAFLKWIGI+VEFEASQVT+A +AS AY+ L
Sbjct: 1151 HHWLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIKVEFEASQVTNALVASKAYVLL 1210

Query: 1264 ADCISISALQDSLGHQFLYKGLLLQALIHPSYHRHGGGCYQRLEFLGDAVLDYLITSYLY 1323
            AD I+ISALQ SLG++FL+KGLLLQAL+HP+YH+HGGGCYQRLEFLGDAVLDYLI SYLY
Sbjct: 1211 ADTINISALQSSLGYKFLHKGLLLQALVHPTYHKHGGGCYQRLEFLGDAVLDYLIMSYLY 1270

Query: 1324 SVYPKLKPGLVTDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIKSPPS 1383
            S YPKLKPGL+TDLRSVFV NEAFAN++VDRFFYKFL+CDS SLQ DIKSYV+FIKS PS
Sbjct: 1271 SAYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLCDSASLQSDIKSYVDFIKSLPS 1330

Query: 1384 ERGSLEQPTCPKVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRD 1443
            ER SLEQP CPK LGD+VES VGAVLVDTGFDMNYVWKIMLSFLD IMS SGFQLSPIRD
Sbjct: 1331 ERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKIMLSFLDPIMSSSGFQLSPIRD 1390

Query: 1444 ITEFCQDCGWKLQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKL 1503
            I E CQ+CGWKLQFN +K+EKY+SV+AEVKG++FH TASA NRRKKDAEKIAAN IL+KL
Sbjct: 1391 IIEVCQNCGWKLQFNPSKMEKYYSVEAEVKGDDFHATASAVNRRKKDAEKIAANSILTKL 1450

Query: 1504 KEKGFIPEVSSLEEILRSRKKMEPELIGFDETPSVSIDQADNGSGTIPGSEHSGAELDPC 1563
            K +GFIPEV+SLE+IL+S +KM+P+LIG+DETPSV++DQ D+      G E SGA+LDP 
Sbjct: 1451 KAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDQIDD------GLEFSGADLDPR 1510

Query: 1564 VNSEFSNSHSVRITRLSEAPEFSSVAAEEELKP---------------STFGGTNLQSSS 1623
            V+ E  NSH  RITR+SE    SS AA E+LKP               S+   ++  SSS
Sbjct: 1511 VHHEVGNSHPFRITRMSETLASSSGAAGEQLKPSIPLEGHDSSTDLLSSSSSSSSSSSSS 1570

Query: 1624 GRSSKTTARSRLYEICAANHWNQPSFDCMNEEGPGHLKMFTYKVVLEIEEAPDTIFEFFG 1683
            GRS KTTARSRL+E+CA NHWN PSF+CMNEEGP HLKMFTYKVVLEIEEAPDTIFEFFG
Sbjct: 1571 GRSGKTTARSRLHEVCAINHWNHPSFNCMNEEGPSHLKMFTYKVVLEIEEAPDTIFEFFG 1630

Query: 1684 ASHSRKKAAAEHAAEAALWYLKKEGYWNETD 1696
            A H  KKAAAEHAAEAALWYLKKE Y  +TD
Sbjct: 1631 APHLTKKAAAEHAAEAALWYLKKERYLGDTD 1653

BLAST of Sed0002244 vs. TAIR 10
Match: AT5G20320.1 (dicer-like 4 )

HSP 1 Score: 1640.6 bits (4247), Expect = 0.0e+00
Identity = 863/1620 (53.27%), Postives = 1126/1620 (69.51%), Query Frame = 0

Query: 84   SLVGDPGAGSSSRASEKDPRRVARKYQLELCKKALEENIIVYLGTGCGKTHIAILLIYEM 143
            SL    G    S   EKDPR++AR+YQ+ELCKKA EEN+IVYLGTGCGKTHIA++LIYE+
Sbjct: 102  SLFSAAGTDDPSPKMEKDPRKIARRYQVELCKKATEENVIVYLGTGCGKTHIAVMLIYEL 161

Query: 144  RNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVYCGGFKGLNSHYDWEREMEEH 203
             +L+   +K +C+FLAPTVALV+QQAKVI DS++FKV ++CGG + + SH +WERE+  +
Sbjct: 162  GHLVLSPKKSVCIFLAPTVALVEQQAKVIADSVNFKVAIHCGGKRIVKSHSEWEREIAAN 221

Query: 204  EVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSDHSYAQIMKVFYKGSVAKRPR 263
            EV VMTPQ LL +L HC+IKME + LLIFDECHHAQ +S+H YA+IMKVFYK    +RPR
Sbjct: 222  EVLVMTPQILLHNLQHCFIKMECISLLIFDECHHAQQQSNHPYAEIMKVFYKSESLQRPR 281

Query: 264  IFGMTASPVVGKGTCNQQSLSKSINSLEKLLDAKVYSVEDREDLEAFVSSPLVKIYYYGP 323
            IFGMTASPVVGKG+   ++LSKSINSLE LL+AKVYSVE    L+ FVSSPLVK+YYY  
Sbjct: 282  IFGMTASPVVGKGSFQSENLSKSINSLENLLNAKVYSVESNVQLDGFVSSPLVKVYYYRS 341

Query: 324  VANGSSSSFMTYCCQLEEVKRQCVVALGQMKGEH--EGLLATKKLLLRMHDNIIFCLENL 383
              + +S S + Y   LE++K++C+ +L  +   H  + LL+ K+LL R HDN+I+ L NL
Sbjct: 342  ALSDASQSTIRYENMLEDIKQRCLASLKLLIDTHQTQTLLSMKRLLKRSHDNLIYTLLNL 401

Query: 384  GVWGALQASKILLSGDNSERSELIEAAEGNPNNDSLSDRYLNQAAEILASGCKIDGGMPD 443
            G+WGA+QA+KI L+ D++ + E +    G      + D YL+ AAE L+SG   D    D
Sbjct: 402  GLWGAIQAAKIQLNSDHNVQDEPV----GKNPKSKICDTYLSMAAEALSSGVAKDENASD 461

Query: 444  MLNVDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIVIARSLSYILQNLKFLAYW 503
            +L++  L++P FS+KL++LI ILS F    +MKCIIFVNRIV AR+LS IL NL+ L  W
Sbjct: 462  LLSLAALKEPLFSRKLVQLIKILSVFRLEPHMKCIIFVNRIVTARTLSCILNNLELLRSW 521

Query: 504  KCDFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPE 563
            K DFLVG+ S L+SMSR++M  IL +F+S ELNLL+ATKVGEEGLDIQTCCLVIR+DLPE
Sbjct: 522  KSDFLVGLSSGLKSMSRRSMETILKRFQSKELNLLVATKVGEEGLDIQTCCLVIRYDLPE 581

Query: 564  TVSSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDENHMNREISSRSSNETFQSH 623
            TV+SFIQSRGRARMPQSEYAF+VD GNEKE  LI+ F+ +E+ MN EI+ RSS ET    
Sbjct: 582  TVTSFIQSRGRARMPQSEYAFLVDSGNEKEMDLIENFKVNEDRMNLEITYRSSEETCPRL 641

Query: 624  EESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKFFY--YDDLEGTICHAILP 683
            +E +Y+V  TGA ++ G SISLL++YCS+LPHDE+F PKP+F +   D+  GTIC   LP
Sbjct: 642  DEELYKVHETGACISGGSSISLLYKYCSRLPHDEFFQPKPEFQFKPVDEFGGTICRITLP 701

Query: 684  SNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPMRDNAIEEELELASCDS 743
            +NAPI ++ S +  S +AAKKDACLKA+ ELH LG  NDFLLP   + IE+EL     D 
Sbjct: 702  ANAPISEIESSLLPSTEAAKKDACLKAVHELHNLGVLNDFLLPDSKDEIEDELSDDEFDF 761

Query: 744  DSSEDE-TSRRELHEMLVPTALKDSWTGSGNVI-LNCYQIKISPHPGDRIYKEFGLFVKA 803
            D+ + E  SR +L+EM VP   K  W  S + + L+ Y I   PHP DRIYK+FG F+K+
Sbjct: 762  DNIKGEGCSRGDLYEMRVPVLFKQKWDPSTSCVNLHSYYIMFVPHPADRIYKKFGFFMKS 821

Query: 804  PLPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFVQ 863
            PLP EAE +D++LHLA  R V V + PSGV E   DEI  AE FQE+ LKV+ +R E + 
Sbjct: 822  PLPVEAETMDIDLHLAHQRSVSVKIFPSGVTEFDNDEIRLAELFQEIALKVLFERGELIP 881

Query: 864  DYVPLR-NNVSGSISSASYLLLPMIFQDNEGSSLIDWKVIRRCLSSQIFQNHACSAVRET 923
            D+VPL   + S +  S  YLLLP+   D E    +DW  IR CLSS IF+  +       
Sbjct: 882  DFVPLELQDSSRTSKSTFYLLLPLCLHDGESVISVDWVTIRNCLSSPIFKTPSVLVEDIF 941

Query: 924  ASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSRYKNSGSLSHFEHL 983
              S +HL L +G   + D++NSLV+  Y ++F+FV  I   +NG+S  K S + SH E +
Sbjct: 942  PPSGSHLKLANGCWNIDDVKNSLVFTTYSKQFYFVADICHGRNGFSPVKESSTKSHVESI 1001

Query: 984  LERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLIDLPPELCQLKITGF 1043
             + + + LK+P QPLL  KPL H+ NLLH+R   + E  +L E+ I++PPEL  LKI G 
Sbjct: 1002 YKLYGVELKHPAQPLLRVKPLCHVRNLLHNRMQTNLEPQELDEYFIEIPPELSHLKIKGL 1061

Query: 1044 TKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRILEALTTEKCQERIS 1103
            +KDIGSS+SLLPS MH +EN LVAIELK  L+A+ P  +EV+ +R+LEALTTEKC ER+S
Sbjct: 1062 SKDIGSSLSLLPSIMHRMENLLVAIELKHVLSASIPEIAEVSGHRVLEALTTEKCHERLS 1121

Query: 1104 LERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFKLATRKNLQVYIRD 1163
            LERLE+LGDAFLKFAV+RHLFL HD  DEGELTRRRS +V N NL +LA +KNLQVYIRD
Sbjct: 1122 LERLEVLGDAFLKFAVSRHLFLHHDSLDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRD 1181

Query: 1164 QPFEPNQFYSLGRPCPRICNEETRKDIHSHKNAKG------GETKCSKGHHWLHKKTISD 1223
            Q  +P QF++ G PC   C+E   K++HS     G      GE +CSKGHHWL+KKTI+D
Sbjct: 1182 QALDPTQFFAFGHPCRVTCDEVASKEVHSLNRDLGILESNTGEIRCSKGHHWLYKKTIAD 1241

Query: 1224 VVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRLADCISISALQD 1283
            VVEALVGAFLVDSGFK A+ FLKWIG+ V+FE+ QV DA +AS  YL L    ++  L++
Sbjct: 1242 VVEALVGAFLVDSGFKGAVKFLKWIGVNVDFESLQVQDACIASRRYLPLTTRNNLETLEN 1301

Query: 1284 SLGHQFLYKGLLLQALIHPSYHRHGGGCYQRLEFLGDAVLDYLITSYLYSVYPKLKPGLV 1343
             L ++FL+KGLL+QA IHPSY+RHGGGCYQRLEFLGDAVLDYL+TSY ++V+PKLKPG +
Sbjct: 1302 QLDYKFLHKGLLVQAFIHPSYNRHGGGCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQL 1361

Query: 1344 TDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIKSPPSERGSLEQPTCP 1403
            TDLRS+ V NEA AN++V     +FL C+S  L + I+ Y NF+ S P   G  E P CP
Sbjct: 1362 TDLRSLSVNNEALANVAVSFSLKRFLFCESIYLHEVIEDYTNFLASSPLASGQSEGPRCP 1421

Query: 1404 KVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRDITEFCQDCGWK 1463
            KVLGD+VES +GA+ +D GF++N+VW +MLSFLD + + S  Q+SPI+++ E CQ   W 
Sbjct: 1422 KVLGDLVESCLGALFLDCGFNLNHVWTMMLSFLDPVKNLSNLQISPIKELIELCQSYKWD 1481

Query: 1464 LQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKLKEKGFIPEVSS 1523
             + +ATK +  F+V+ +V  N    T SA  R K++  K AA L+++ LK    I     
Sbjct: 1482 REISATKKDGAFTVELKVTKNGCCLTVSATGRNKREGTKKAAQLMITNLKAHENITTSHP 1541

Query: 1524 LEEILRSRKKMEPELIGFDETPSVSIDQADNGSGTIPGSEHSGAELDPCVNSEFSNSHSV 1583
            LE++L++  + E +LIG++E P   +D        +   E  G       NSE S+S+ +
Sbjct: 1542 LEDVLKNGIRNEAKLIGYNEDPIDVVDLVGLDVENLNILETFGG------NSERSSSYVI 1601

Query: 1584 RITRLSEAPEFSSVAAEEELKPSTFGGTNLQSSSGRSSKTTARSRLYEICAANHWNQPSF 1643
            R   L +AP       E+ L         +  + G SSK TA+S L+E C AN W  P F
Sbjct: 1602 R-RGLPQAPS----KTEDRLPQKA-----IIKAGGPSSK-TAKSLLHETCVANCWKPPHF 1661

Query: 1644 DCMNEEGPGHLKMFTYKVVLEIEEAPDTIFEFFGASHSRKKAAAEHAAEAALWYLKKEGY 1691
            +C  EEGPGHLK F YKV+LE+E+AP+   E +G + + KK AAEHAA+AA+W LK  G+
Sbjct: 1662 ECCEEEGPGHLKSFVYKVILEVEDAPNMTLECYGEARATKKGAAEHAAQAAIWCLKHSGF 1700

BLAST of Sed0002244 vs. TAIR 10
Match: AT5G20320.2 (dicer-like 4 )

HSP 1 Score: 1614.0 bits (4178), Expect = 0.0e+00
Identity = 854/1620 (52.72%), Postives = 1116/1620 (68.89%), Query Frame = 0

Query: 84   SLVGDPGAGSSSRASEKDPRRVARKYQLELCKKALEENIIVYLGTGCGKTHIAILLIYEM 143
            SL    G    S   EKDPR++AR+YQ+ELCKKA EEN+IVYLGTGCGKTHIA++LIYE+
Sbjct: 102  SLFSAAGTDDPSPKMEKDPRKIARRYQVELCKKATEENVIVYLGTGCGKTHIAVMLIYEL 161

Query: 144  RNLIRRSQKGICVFLAPTVALVQQQAKVIEDSIDFKVRVYCGGFKGLNSHYDWEREMEEH 203
             +L+   +K +C+FLAPTVALV+QQAKVI DS++FKV ++CGG + + SH +WERE+  +
Sbjct: 162  GHLVLSPKKSVCIFLAPTVALVEQQAKVIADSVNFKVAIHCGGKRIVKSHSEWEREIAAN 221

Query: 204  EVFVMTPQTLLRSLNHCYIKMEFVELLIFDECHHAQVKSDHSYAQIMKVFYKGSVAKRPR 263
            E              HC+IKME + LLIFDECHHAQ +S+H YA+IMKVFYK    +RPR
Sbjct: 222  E--------------HCFIKMECISLLIFDECHHAQQQSNHPYAEIMKVFYKSESLQRPR 281

Query: 264  IFGMTASPVVGKGTCNQQSLSKSINSLEKLLDAKVYSVEDREDLEAFVSSPLVKIYYYGP 323
            IFGMTASPVVGKG+   ++LSKSINSLE LL+AKVYSVE    L+ FVSSPLVK+YYY  
Sbjct: 282  IFGMTASPVVGKGSFQSENLSKSINSLENLLNAKVYSVESNVQLDGFVSSPLVKVYYYRS 341

Query: 324  VANGSSSSFMTYCCQLEEVKRQCVVALGQMKGEH--EGLLATKKLLLRMHDNIIFCLENL 383
              + +S S + Y   LE++K++C+ +L  +   H  + LL+ K+LL R HDN+I+ L NL
Sbjct: 342  ALSDASQSTIRYENMLEDIKQRCLASLKLLIDTHQTQTLLSMKRLLKRSHDNLIYTLLNL 401

Query: 384  GVWGALQASKILLSGDNSERSELIEAAEGNPNNDSLSDRYLNQAAEILASGCKIDGGMPD 443
            G+WGA+QA+KI L+ D++ + E +    G      + D YL+ AAE L+SG   D    D
Sbjct: 402  GLWGAIQAAKIQLNSDHNVQDEPV----GKNPKSKICDTYLSMAAEALSSGVAKDENASD 461

Query: 444  MLNVDILEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIVIARSLSYILQNLKFLAYW 503
            +L++  L++P FS+KL++LI ILS F    +MKCIIFVNRIV AR+LS IL NL+ L  W
Sbjct: 462  LLSLAALKEPLFSRKLVQLIKILSVFRLEPHMKCIIFVNRIVTARTLSCILNNLELLRSW 521

Query: 504  KCDFLVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPE 563
            K DFLVG+ S L+SMSR++M  IL +F+S ELNLL+ATKVGEEGLDIQTCCLVIR+DLPE
Sbjct: 522  KSDFLVGLSSGLKSMSRRSMETILKRFQSKELNLLVATKVGEEGLDIQTCCLVIRYDLPE 581

Query: 564  TVSSFIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDENHMNREISSRSSNETFQSH 623
            TV+SFIQSRGRARMPQSEYAF+VD GNEKE  LI+ F+ +E+ MN EI+ RSS ET    
Sbjct: 582  TVTSFIQSRGRARMPQSEYAFLVDSGNEKEMDLIENFKVNEDRMNLEITYRSSEETCPRL 641

Query: 624  EESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKFFY--YDDLEGTICHAILP 683
            +E +Y+V  TGA ++ G SISLL++YCS+LPHDE+F PKP+F +   D+  GTIC   LP
Sbjct: 642  DEELYKVHETGACISGGSSISLLYKYCSRLPHDEFFQPKPEFQFKPVDEFGGTICRITLP 701

Query: 684  SNAPIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPMRDNAIEEELELASCDS 743
            +NAPI ++ S +  S +AAKKDACLKA+ ELH LG  NDFLLP   + IE+EL     D 
Sbjct: 702  ANAPISEIESSLLPSTEAAKKDACLKAVHELHNLGVLNDFLLPDSKDEIEDELSDDEFDF 761

Query: 744  DSSEDE-TSRRELHEMLVPTALKDSWTGSGNVI-LNCYQIKISPHPGDRIYKEFGLFVKA 803
            D+ + E  SR +L+EM VP   K  W  S + + L+ Y I   PHP DRIYK+FG F+K+
Sbjct: 762  DNIKGEGCSRGDLYEMRVPVLFKQKWDPSTSCVNLHSYYIMFVPHPADRIYKKFGFFMKS 821

Query: 804  PLPQEAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFVQ 863
            PLP EAE +D++LHLA  R V V + PSGV E   DEI  AE FQE+ LKV+ +R E + 
Sbjct: 822  PLPVEAETMDIDLHLAHQRSVSVKIFPSGVTEFDNDEIRLAELFQEIALKVLFERGELIP 881

Query: 864  DYVPLR-NNVSGSISSASYLLLPMIFQDNEGSSLIDWKVIRRCLSSQIFQNHACSAVRET 923
            D+VPL   + S +  S  YLLLP+   D E    +DW  IR CLSS IF+  +       
Sbjct: 882  DFVPLELQDSSRTSKSTFYLLLPLCLHDGESVISVDWVTIRNCLSSPIFKTPSVLVEDIF 941

Query: 924  ASSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYSRYKNSGSLSHFEHL 983
              S +HL L +G   + D++NSLV+  Y ++F+FV  I   +NG+S  K S + SH E +
Sbjct: 942  PPSGSHLKLANGCWNIDDVKNSLVFTTYSKQFYFVADICHGRNGFSPVKESSTKSHVESI 1001

Query: 984  LERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLIDLPPELCQLKITGF 1043
             + + + LK+P QPLL  KPL H+ NLLH+R   + E  +L E+ I++PPEL  LKI G 
Sbjct: 1002 YKLYGVELKHPAQPLLRVKPLCHVRNLLHNRMQTNLEPQELDEYFIEIPPELSHLKIKGL 1061

Query: 1044 TKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRILEALTTEKCQERIS 1103
            +KDIGSS+SLLPS MH +EN LVAIELK  L+A+ P  +EV+ +R+LEALTTEKC ER+S
Sbjct: 1062 SKDIGSSLSLLPSIMHRMENLLVAIELKHVLSASIPEIAEVSGHRVLEALTTEKCHERLS 1121

Query: 1104 LERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFKLATRKNLQVYIRD 1163
            LERLE+LGDAFLKFAV+RHLFL HD  DEGELTRRRS +V N NL +LA +KNLQVYIRD
Sbjct: 1122 LERLEVLGDAFLKFAVSRHLFLHHDSLDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRD 1181

Query: 1164 QPFEPNQFYSLGRPCPRICNEETRKDIHSHKNAKG------GETKCSKGHHWLHKKTISD 1223
            Q  +P QF++ G PC   C+E   K++HS     G      GE +CSKGHHWL+KKTI+D
Sbjct: 1182 QALDPTQFFAFGHPCRVTCDEVASKEVHSLNRDLGILESNTGEIRCSKGHHWLYKKTIAD 1241

Query: 1224 VVEALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRLADCISISALQD 1283
            VVEALVGAFLVDSGFK A+ FLKWIG+ V+FE+ QV DA +AS  YL L    ++  L++
Sbjct: 1242 VVEALVGAFLVDSGFKGAVKFLKWIGVNVDFESLQVQDACIASRRYLPLTTRNNLETLEN 1301

Query: 1284 SLGHQFLYKGLLLQALIHPSYHRHGGGCYQRLEFLGDAVLDYLITSYLYSVYPKLKPGLV 1343
             L ++FL+KGLL+QA IHPSY+RHGGGCYQRLEFLGDAVLDYL+TSY ++V+PKLKPG +
Sbjct: 1302 QLDYKFLHKGLLVQAFIHPSYNRHGGGCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQL 1361

Query: 1344 TDLRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIKSPPSERGSLEQPTCP 1403
            TDLRS+ V NEA AN++V     +FL C+S  L + I+ Y NF+ S P   G  E P CP
Sbjct: 1362 TDLRSLSVNNEALANVAVSFSLKRFLFCESIYLHEVIEDYTNFLASSPLASGQSEGPRCP 1421

Query: 1404 KVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRDITEFCQDCGWK 1463
            KVLGD+VES +GA+ +D GF++N+VW +MLSFLD + + S  Q+SPI+++ E CQ   W 
Sbjct: 1422 KVLGDLVESCLGALFLDCGFNLNHVWTMMLSFLDPVKNLSNLQISPIKELIELCQSYKWD 1481

Query: 1464 LQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKLKEKGFIPEVSS 1523
             + +ATK +  F+V+ +V  N    T SA  R K++  K AA L+++ LK    I     
Sbjct: 1482 REISATKKDGAFTVELKVTKNGCCLTVSATGRNKREGTKKAAQLMITNLKAHENITTSHP 1541

Query: 1524 LEEILRSRKKMEPELIGFDETPSVSIDQADNGSGTIPGSEHSGAELDPCVNSEFSNSHSV 1583
            LE++L++  + E +LIG++E P   +D        +   E  G       NSE S+S+ +
Sbjct: 1542 LEDVLKNGIRNEAKLIGYNEDPIDVVDLVGLDVENLNILETFGG------NSERSSSYVI 1601

Query: 1584 RITRLSEAPEFSSVAAEEELKPSTFGGTNLQSSSGRSSKTTARSRLYEICAANHWNQPSF 1643
            R   L +AP       E+ L         +  + G SSK TA+S L+E C AN W  P F
Sbjct: 1602 R-RGLPQAPS----KTEDRLPQKA-----IIKAGGPSSK-TAKSLLHETCVANCWKPPHF 1661

Query: 1644 DCMNEEGPGHLKMFTYKVVLEIEEAPDTIFEFFGASHSRKKAAAEHAAEAALWYLKKEGY 1691
            +C  EEGPGHLK F YKV+LE+E+AP+   E +G + + KK AAEHAA+AA+W LK  G+
Sbjct: 1662 ECCEEEGPGHLKSFVYKVILEVEDAPNMTLECYGEARATKKGAAEHAAQAAIWCLKHSGF 1686

BLAST of Sed0002244 vs. TAIR 10
Match: AT3G03300.1 (dicer-like 2 )

HSP 1 Score: 664.1 bits (1712), Expect = 3.1e-190
Identity = 469/1430 (32.80%), Postives = 759/1430 (53.08%), Query Frame = 0

Query: 102  PRRVARKYQLELCKKALEENIIVYLGTGCGKTHIAILLIYEMRNLIRRSQKGICVFLAPT 161
            P   AR YQ+E  +KA+++N IV+L TG GKT IAI+L+     L R+     CVFL P 
Sbjct: 20   PLHFARSYQVEALEKAIKQNTIVFLETGSGKTLIAIMLLRSYAYLFRKPSPCFCVFLVPQ 79

Query: 162  VALVQQQAKVIEDSIDFKVRVYCGGFKGLN--SHYDWEREMEEHEVFVMTPQTLLRSLNH 221
            V LV QQA+ ++   D KV +Y G   G++      W++E++++EV VMTP  LL +L H
Sbjct: 80   VVLVTQQAEALKMHTDLKVGMYWGDM-GVDFWDSSTWKQEVDKYEVLVMTPAILLDALRH 139

Query: 222  CYIKMEFVELLIFDECHHAQVKSDHSYAQIMKVFY----KGSVAKRPRIFGMTASPVVGK 281
             ++ +  +++LI DECHHA  K  H YA IM+ FY        +  PRIFGMTAS V  K
Sbjct: 140  SFLSLSMIKVLIVDECHHAGGK--HPYACIMREFYHKELNSGTSNVPRIFGMTASLVKTK 199

Query: 282  GTCNQQSLSKSINSLEKLLDAKVYSVEDREDLEAFV--SSPLVKIYYYGPVANGSSSSFM 341
            G  N  S  K I+ LE L+++KVY+ E+   L  FV  S+P  K Y +  + +   +S +
Sbjct: 200  GE-NLDSYWKKIHELETLMNSKVYTCENESVLAGFVPFSTPSFKYYQHIKIPSPKRASLV 259

Query: 342  TYCCQLEEVKRQCVVALGQMKGEHEGLLATKKLLLRMHDNIIFCLENLGVWGALQASKIL 401
                +LE +  +  ++LG +      + + +K LLR+   + +CL++LG+  A +A++ L
Sbjct: 260  E---KLERLTIKHRLSLGTLDLNSSTVDSVEKRLLRISSTLTYCLDDLGILLAQKAAQSL 319

Query: 402  LSGDNSERSELIEAAEGNPNNDSLSDRYLNQAA-EILASGCKIDGGMPDMLNVDI----- 461
             +  N    +     E N  + +L  ++ + A+ E LA        +P  LN  +     
Sbjct: 320  SASQN----DSFLWGELNMFSVALVKKFCSDASQEFLAE-------IPQGLNWSVANING 379

Query: 462  -LEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIVIARSL-SYILQNLKFLAYWKCDF 521
              E    + K + LI  L  +    N++CIIFV+R++ A  L S + + L     WK  +
Sbjct: 380  NAEAGLLTLKTVCLIETLLGYSSLENIRCIIFVDRVITAIVLESLLAEILPNCNNWKTKY 439

Query: 522  LVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSS 581
            + G +S L++ +RK  N I+  FR G +N+++AT + EEGLD+Q+C LVIRFD    + S
Sbjct: 440  VAGNNSGLQNQTRKKQNEIVEDFRRGLVNIIVATSILEEGLDVQSCNLVIRFDPASNICS 499

Query: 582  FIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDENHMNREISSRS---SNETFQSHE 641
            FIQSRGRARM  S+Y  +V+ G+   +  +  +      M  E    S           +
Sbjct: 500  FIQSRGRARMQNSDYLMMVESGDLLTQSRLMKYLSGGKRMREESLDHSLVPCPPLPDDSD 559

Query: 642  ESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKFFYYDDLEGTICHAILPSNA 701
            E ++RV STGA+VT   S+SL++ YCS+LP DEYF P P+F    D +G+ C   LP + 
Sbjct: 560  EPLFRVESTGATVTLSSSVSLIYHYCSRLPSDEYFKPAPRFDVNKD-QGS-CTLYLPKSC 619

Query: 702  PIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPMRDNAIEEELELASCDSDSS 761
            P+ +V  + + +    K+  CLKA  +LHK+GA +D L+P        ++ +A   S   
Sbjct: 620  PVKEV--KAEANNKVLKQAVCLKACIQLHKVGALSDHLVP--------DMVVAETVSQKL 679

Query: 762  EDETSRRELHEMLVPTALKDSWTGSGNVILNCYQIKISPH-PGDRIYKEFGLFVKAPLPQ 821
            E      E      P  L   ++       + Y I++ P+ P +    +  L  +  L  
Sbjct: 680  EKIQYNTE-QPCYFPPELVSQFSAQPETTYHFYLIRMKPNSPRNFHLNDVLLGTRVVLED 739

Query: 822  EAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFVQDYVP 881
            +       L   R   + V L   G   L ++E+     FQ    +V+LD S  V++ + 
Sbjct: 740  DIGNTSFRLEDHRGT-IAVTLSYVGAFHLTQEEVLFCRRFQITLFRVLLDHS--VENLME 799

Query: 882  LRNNVSGSISSA-SYLLLPMIFQDNEGSSLIDWKVIR--RCLSSQIFQNHA-CSAVRETA 941
              N +      A  YLL+P     +  +SLIDW+VIR     S ++ + H  CS    T 
Sbjct: 800  ALNGLHLRDGVALDYLLVPS--THSHETSLIDWEVIRSVNLTSHEVLEKHENCS----TN 859

Query: 942  SSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYS--RYKNSGSLSHFEH 1001
             +   L   DG    C ++N+LVY P+    +    ++ + NG S    +NSG  ++ E+
Sbjct: 860  GASRILHTKDGLFCTCVVQNALVYTPHNGYVYCTKGVLNNLNGNSLLTKRNSGDQTYIEY 919

Query: 1002 LLERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLIDLPPELCQLKITG 1061
              ER  I L + ++PLL  + +F LH+ LH  +    +  D     ++LPPELC + ++ 
Sbjct: 920  YEERHGIQLNFVDEPLLNGRHIFTLHSYLHMAKKKKEKEHD--REFVELPPELCHVILSP 979

Query: 1062 FTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRILEALTTEKCQERI 1121
             + D+  S + +PS M  +E+ L+A  LK ++         + + ++LEA+TT+KC+++ 
Sbjct: 980  ISVDMIYSYTFIPSVMQRIESLLIAYNLKKSIPKV-----NIPTIKVLEAITTKKCEDQF 1039

Query: 1122 SLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFKLATRKNLQVYIR 1181
             LE LE LGD+FLK+AV + LF       EG L+ ++  ++ N+ L +   ++ LQ +IR
Sbjct: 1040 HLESLETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVMLCQFGCQQKLQGFIR 1099

Query: 1182 DQPFEPNQFYSLGRPCP--RICNEETRKDIHSHKNAKGGETKCSKGHHWLHKKTISDVVE 1241
            D+ FEP  +   G+      + N+   +  + +  ++            L +K+++DVVE
Sbjct: 1100 DECFEPKGWMVPGQSSAAYSLVNDTLPESRNIYVASRRN----------LKRKSVADVVE 1159

Query: 1242 ALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRLADCISISALQDSLG 1301
            +L+GA+L + G  AA+ F+ W+GI+V+F     T  ++  ++ ++    +++  ++  L 
Sbjct: 1160 SLIGAYLSEGGELAALMFMNWVGIKVDF-----TTTKIQRDSPIQAEKLVNVGYMESLLN 1219

Query: 1302 HQFLYKGLLLQALIHPSYHR-HGGGCYQRLEFLGDAVLDYLITSYLYSVYPKLKPGLVTD 1361
            + F  K LL++AL H SY       CYQRLEFLGD+VLDYLIT +LY  YP L PGL+TD
Sbjct: 1220 YSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLSPGLLTD 1279

Query: 1362 LRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIK--SPPSERGSLEQPTCP 1421
            +RS  V NE +A ++V    +K ++  S  L K I   V+  +  S  S  G     + P
Sbjct: 1280 MRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWESDISFP 1339

Query: 1422 KVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRDITEFCQDCGWK 1481
            KVLGD++ES+ GA+ VD+G++   V+  +   L  +++    +L P+R++TE CQ   W+
Sbjct: 1340 KVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELCQK--WQ 1383

Query: 1482 LQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKLK 1501
             + +  K    F+V+ + K  +F  TA A++  KK A+K+A   +L+ LK
Sbjct: 1400 FELSKAKDFDSFTVEVKAKEMSFAHTAKASD--KKMAKKLAYKEVLNLLK 1383

BLAST of Sed0002244 vs. TAIR 10
Match: AT3G03300.3 (dicer-like 2 )

HSP 1 Score: 664.1 bits (1712), Expect = 3.1e-190
Identity = 469/1430 (32.80%), Postives = 759/1430 (53.08%), Query Frame = 0

Query: 102  PRRVARKYQLELCKKALEENIIVYLGTGCGKTHIAILLIYEMRNLIRRSQKGICVFLAPT 161
            P   AR YQ+E  +KA+++N IV+L TG GKT IAI+L+     L R+     CVFL P 
Sbjct: 20   PLHFARSYQVEALEKAIKQNTIVFLETGSGKTLIAIMLLRSYAYLFRKPSPCFCVFLVPQ 79

Query: 162  VALVQQQAKVIEDSIDFKVRVYCGGFKGLN--SHYDWEREMEEHEVFVMTPQTLLRSLNH 221
            V LV QQA+ ++   D KV +Y G   G++      W++E++++EV VMTP  LL +L H
Sbjct: 80   VVLVTQQAEALKMHTDLKVGMYWGDM-GVDFWDSSTWKQEVDKYEVLVMTPAILLDALRH 139

Query: 222  CYIKMEFVELLIFDECHHAQVKSDHSYAQIMKVFY----KGSVAKRPRIFGMTASPVVGK 281
             ++ +  +++LI DECHHA  K  H YA IM+ FY        +  PRIFGMTAS V  K
Sbjct: 140  SFLSLSMIKVLIVDECHHAGGK--HPYACIMREFYHKELNSGTSNVPRIFGMTASLVKTK 199

Query: 282  GTCNQQSLSKSINSLEKLLDAKVYSVEDREDLEAFV--SSPLVKIYYYGPVANGSSSSFM 341
            G  N  S  K I+ LE L+++KVY+ E+   L  FV  S+P  K Y +  + +   +S +
Sbjct: 200  GE-NLDSYWKKIHELETLMNSKVYTCENESVLAGFVPFSTPSFKYYQHIKIPSPKRASLV 259

Query: 342  TYCCQLEEVKRQCVVALGQMKGEHEGLLATKKLLLRMHDNIIFCLENLGVWGALQASKIL 401
                +LE +  +  ++LG +      + + +K LLR+   + +CL++LG+  A +A++ L
Sbjct: 260  E---KLERLTIKHRLSLGTLDLNSSTVDSVEKRLLRISSTLTYCLDDLGILLAQKAAQSL 319

Query: 402  LSGDNSERSELIEAAEGNPNNDSLSDRYLNQAA-EILASGCKIDGGMPDMLNVDI----- 461
             +  N    +     E N  + +L  ++ + A+ E LA        +P  LN  +     
Sbjct: 320  SASQN----DSFLWGELNMFSVALVKKFCSDASQEFLAE-------IPQGLNWSVANING 379

Query: 462  -LEDPFFSKKLLRLIGILSSFGHRLNMKCIIFVNRIVIARSL-SYILQNLKFLAYWKCDF 521
              E    + K + LI  L  +    N++CIIFV+R++ A  L S + + L     WK  +
Sbjct: 380  NAEAGLLTLKTVCLIETLLGYSSLENIRCIIFVDRVITAIVLESLLAEILPNCNNWKTKY 439

Query: 522  LVGVHSKLRSMSRKTMNRILTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSS 581
            + G +S L++ +RK  N I+  FR G +N+++AT + EEGLD+Q+C LVIRFD    + S
Sbjct: 440  VAGNNSGLQNQTRKKQNEIVEDFRRGLVNIIVATSILEEGLDVQSCNLVIRFDPASNICS 499

Query: 582  FIQSRGRARMPQSEYAFIVDRGNEKERGLIDGFQKDENHMNREISSRS---SNETFQSHE 641
            FIQSRGRARM  S+Y  +V+ G+   +  +  +      M  E    S           +
Sbjct: 500  FIQSRGRARMQNSDYLMMVESGDLLTQSRLMKYLSGGKRMREESLDHSLVPCPPLPDDSD 559

Query: 642  ESIYRVVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKFFYYDDLEGTICHAILPSNA 701
            E ++RV STGA+VT   S+SL++ YCS+LP DEYF P P+F    D +G+ C   LP + 
Sbjct: 560  EPLFRVESTGATVTLSSSVSLIYHYCSRLPSDEYFKPAPRFDVNKD-QGS-CTLYLPKSC 619

Query: 702  PIPQVVSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPMRDNAIEEELELASCDSDSS 761
            P+ +V  + + +    K+  CLKA  +LHK+GA +D L+P        ++ +A   S   
Sbjct: 620  PVKEV--KAEANNKVLKQAVCLKACIQLHKVGALSDHLVP--------DMVVAETVSQKL 679

Query: 762  EDETSRRELHEMLVPTALKDSWTGSGNVILNCYQIKISPH-PGDRIYKEFGLFVKAPLPQ 821
            E      E      P  L   ++       + Y I++ P+ P +    +  L  +  L  
Sbjct: 680  EKIQYNTE-QPCYFPPELVSQFSAQPETTYHFYLIRMKPNSPRNFHLNDVLLGTRVVLED 739

Query: 822  EAERIDLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFVQDYVP 881
            +       L   R   + V L   G   L ++E+     FQ    +V+LD S  V++ + 
Sbjct: 740  DIGNTSFRLEDHRGT-IAVTLSYVGAFHLTQEEVLFCRRFQITLFRVLLDHS--VENLME 799

Query: 882  LRNNVSGSISSA-SYLLLPMIFQDNEGSSLIDWKVIR--RCLSSQIFQNHA-CSAVRETA 941
              N +      A  YLL+P     +  +SLIDW+VIR     S ++ + H  CS    T 
Sbjct: 800  ALNGLHLRDGVALDYLLVPS--THSHETSLIDWEVIRSVNLTSHEVLEKHENCS----TN 859

Query: 942  SSDTHLTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYS--RYKNSGSLSHFEH 1001
             +   L   DG    C ++N+LVY P+    +    ++ + NG S    +NSG  ++ E+
Sbjct: 860  GASRILHTKDGLFCTCVVQNALVYTPHNGYVYCTKGVLNNLNGNSLLTKRNSGDQTYIEY 919

Query: 1002 LLERFDIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLIDLPPELCQLKITG 1061
              ER  I L + ++PLL  + +F LH+ LH  +    +  D     ++LPPELC + ++ 
Sbjct: 920  YEERHGIQLNFVDEPLLNGRHIFTLHSYLHMAKKKKEKEHD--REFVELPPELCHVILSP 979

Query: 1062 FTKDIGSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRILEALTTEKCQERI 1121
             + D+  S + +PS M  +E+ L+A  LK ++         + + ++LEA+TT+KC+++ 
Sbjct: 980  ISVDMIYSYTFIPSVMQRIESLLIAYNLKKSIPKV-----NIPTIKVLEAITTKKCEDQF 1039

Query: 1122 SLERLEILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFKLATRKNLQVYIR 1181
             LE LE LGD+FLK+AV + LF       EG L+ ++  ++ N+ L +   ++ LQ +IR
Sbjct: 1040 HLESLETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVMLCQFGCQQKLQGFIR 1099

Query: 1182 DQPFEPNQFYSLGRPCP--RICNEETRKDIHSHKNAKGGETKCSKGHHWLHKKTISDVVE 1241
            D+ FEP  +   G+      + N+   +  + +  ++            L +K+++DVVE
Sbjct: 1100 DECFEPKGWMVPGQSSAAYSLVNDTLPESRNIYVASRRN----------LKRKSVADVVE 1159

Query: 1242 ALVGAFLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRLADCISISALQDSLG 1301
            +L+GA+L + G  AA+ F+ W+GI+V+F     T  ++  ++ ++    +++  ++  L 
Sbjct: 1160 SLIGAYLSEGGELAALMFMNWVGIKVDF-----TTTKIQRDSPIQAEKLVNVGYMESLLN 1219

Query: 1302 HQFLYKGLLLQALIHPSYHR-HGGGCYQRLEFLGDAVLDYLITSYLYSVYPKLKPGLVTD 1361
            + F  K LL++AL H SY       CYQRLEFLGD+VLDYLIT +LY  YP L PGL+TD
Sbjct: 1220 YSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLSPGLLTD 1279

Query: 1362 LRSVFVMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIK--SPPSERGSLEQPTCP 1421
            +RS  V NE +A ++V    +K ++  S  L K I   V+  +  S  S  G     + P
Sbjct: 1280 MRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWESDISFP 1339

Query: 1422 KVLGDMVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRDITEFCQDCGWK 1481
            KVLGD++ES+ GA+ VD+G++   V+  +   L  +++    +L P+R++TE CQ   W+
Sbjct: 1340 KVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELCQK--WQ 1383

Query: 1482 LQFNATKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKLK 1501
             + +  K    F+V+ + K  +F  TA A++  KK A+K+A   +L+ LK
Sbjct: 1400 FELSKAKDFDSFTVEVKAKEMSFAHTAKASD--KKMAKKLAYKEVLNLLK 1383

BLAST of Sed0002244 vs. TAIR 10
Match: AT3G03300.2 (dicer-like 2 )

HSP 1 Score: 608.2 bits (1567), Expect = 2.0e-173
Identity = 438/1365 (32.09%), Postives = 719/1365 (52.67%), Query Frame = 0

Query: 167  QQAKVIEDSIDFKVRVYCGGFKGLN--SHYDWEREMEEHEVFVMTPQTLLRSLNHCYIKM 226
            QQA+ ++   D KV +Y G   G++      W++E++++EV VMTP  LL +L H ++ +
Sbjct: 71   QQAEALKMHTDLKVGMYWGDM-GVDFWDSSTWKQEVDKYEVLVMTPAILLDALRHSFLSL 130

Query: 227  EFVELLIFDECHHAQVKSDHSYAQIMKVFY----KGSVAKRPRIFGMTASPVVGKGTCNQ 286
              +++LI DECHHA  K  H YA IM+ FY        +  PRIFGMTAS V  KG  N 
Sbjct: 131  SMIKVLIVDECHHAGGK--HPYACIMREFYHKELNSGTSNVPRIFGMTASLVKTKGE-NL 190

Query: 287  QSLSKSINSLEKLLDAKVYSVEDREDLEAFV--SSPLVKIYYYGPVANGSSSSFMTYCCQ 346
             S  K I+ LE L+++KVY+ E+   L  FV  S+P  K Y +  + +   +S +    +
Sbjct: 191  DSYWKKIHELETLMNSKVYTCENESVLAGFVPFSTPSFKYYQHIKIPSPKRASLVE---K 250

Query: 347  LEEVKRQCVVALGQMKGEHEGLLATKKLLLRMHDNIIFCLENLGVWGALQASKILLSGDN 406
            LE +  +  ++LG +      + + +K LLR+   + +CL++LG+  A +A++ L +  N
Sbjct: 251  LERLTIKHRLSLGTLDLNSSTVDSVEKRLLRISSTLTYCLDDLGILLAQKAAQSLSASQN 310

Query: 407  SERSELIEAAEGNPNNDSLSDRYLNQAA-EILASGCKIDGGMPDMLNVDI------LEDP 466
                +     E N  + +L  ++ + A+ E LA        +P  LN  +       E  
Sbjct: 311  ----DSFLWGELNMFSVALVKKFCSDASQEFLAE-------IPQGLNWSVANINGNAEAG 370

Query: 467  FFSKKLLRLIGILSSFGHRLNMKCIIFVNRIVIARSL-SYILQNLKFLAYWKCDFLVGVH 526
              + K + LI  L  +    N++CIIFV+R++ A  L S + + L     WK  ++ G +
Sbjct: 371  LLTLKTVCLIETLLGYSSLENIRCIIFVDRVITAIVLESLLAEILPNCNNWKTKYVAGNN 430

Query: 527  SKLRSMSRKTMNRILTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSR 586
            S L++ +RK  N I+  FR G +N+++AT + EEGLD+Q+C LVIRFD    + SFIQSR
Sbjct: 431  SGLQNQTRKKQNEIVEDFRRGLVNIIVATSILEEGLDVQSCNLVIRFDPASNICSFIQSR 490

Query: 587  GRARMPQSEYAFIVDRGNEKERGLIDGFQKDENHMNREISSRS---SNETFQSHEESIYR 646
            GRARM  S+Y  +V+ G+   +  +  +      M  E    S           +E ++R
Sbjct: 491  GRARMQNSDYLMMVESGDLLTQSRLMKYLSGGKRMREESLDHSLVPCPPLPDDSDEPLFR 550

Query: 647  VVSTGASVTSGLSISLLHEYCSKLPHDEYFDPKPKFFYYDDLEGTICHAILPSNAPIPQV 706
            V STGA+VT   S+SL++ YCS+LP DEYF P P+F    D +G+ C   LP + P+ +V
Sbjct: 551  VESTGATVTLSSSVSLIYHYCSRLPSDEYFKPAPRFDVNKD-QGS-CTLYLPKSCPVKEV 610

Query: 707  VSRVQFSRDAAKKDACLKAIEELHKLGAFNDFLLPMRDNAIEEELELASCDSDSSEDETS 766
              + + +    K+  CLKA  +LHK+GA +D L+P        ++ +A   S   E    
Sbjct: 611  --KAEANNKVLKQAVCLKACIQLHKVGALSDHLVP--------DMVVAETVSQKLEKIQY 670

Query: 767  RRELHEMLVPTALKDSWTGSGNVILNCYQIKISPH-PGDRIYKEFGLFVKAPLPQEAERI 826
              E      P  L   ++       + Y I++ P+ P +    +  L  +  L  +    
Sbjct: 671  NTE-QPCYFPPELVSQFSAQPETTYHFYLIRMKPNSPRNFHLNDVLLGTRVVLEDDIGNT 730

Query: 827  DLELHLARSRYVKVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFVQDYVPLRNNV 886
               L   R   + V L   G   L ++E+     FQ    +V+LD S  V++ +   N +
Sbjct: 731  SFRLEDHRGT-IAVTLSYVGAFHLTQEEVLFCRRFQITLFRVLLDHS--VENLMEALNGL 790

Query: 887  SGSISSA-SYLLLPMIFQDNEGSSLIDWKVIR--RCLSSQIFQNHA-CSAVRETASSDTH 946
                  A  YLL+P     +  +SLIDW+VIR     S ++ + H  CS    T  +   
Sbjct: 791  HLRDGVALDYLLVPS--THSHETSLIDWEVIRSVNLTSHEVLEKHENCS----TNGASRI 850

Query: 947  LTLYDGRRRLCDIENSLVYVPYKREFFFVTHIVRSKNGYS--RYKNSGSLSHFEHLLERF 1006
            L   DG    C ++N+LVY P+    +    ++ + NG S    +NSG  ++ E+  ER 
Sbjct: 851  LHTKDGLFCTCVVQNALVYTPHNGYVYCTKGVLNNLNGNSLLTKRNSGDQTYIEYYEERH 910

Query: 1007 DIHLKYPEQPLLCAKPLFHLHNLLHDRRLNDSEACDLIEHLIDLPPELCQLKITGFTKDI 1066
             I L + ++PLL  + +F LH+ LH  +    +  D     ++LPPELC + ++  + D+
Sbjct: 911  GIQLNFVDEPLLNGRHIFTLHSYLHMAKKKKEKEHD--REFVELPPELCHVILSPISVDM 970

Query: 1067 GSSISLLPSFMHHLENFLVAIELKCTLAAAFPAASEVTSNRILEALTTEKCQERISLERL 1126
              S + +PS M  +E+ L+A  LK ++         + + ++LEA+TT+KC+++  LE L
Sbjct: 971  IYSYTFIPSVMQRIESLLIAYNLKKSIPKV-----NIPTIKVLEAITTKKCEDQFHLESL 1030

Query: 1127 EILGDAFLKFAVARHLFLTHDKFDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFE 1186
            E LGD+FLK+AV + LF       EG L+ ++  ++ N+ L +   ++ LQ +IRD+ FE
Sbjct: 1031 ETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVMLCQFGCQQKLQGFIRDECFE 1090

Query: 1187 PNQFYSLGRPCP--RICNEETRKDIHSHKNAKGGETKCSKGHHWLHKKTISDVVEALVGA 1246
            P  +   G+      + N+   +  + +  ++            L +K+++DVVE+L+GA
Sbjct: 1091 PKGWMVPGQSSAAYSLVNDTLPESRNIYVASRRN----------LKRKSVADVVESLIGA 1150

Query: 1247 FLVDSGFKAAIAFLKWIGIQVEFEASQVTDARMASNAYLRLADCISISALQDSLGHQFLY 1306
            +L + G  AA+ F+ W+GI+V+F     T  ++  ++ ++    +++  ++  L + F  
Sbjct: 1151 YLSEGGELAALMFMNWVGIKVDF-----TTTKIQRDSPIQAEKLVNVGYMESLLNYSFED 1210

Query: 1307 KGLLLQALIHPSYHR-HGGGCYQRLEFLGDAVLDYLITSYLYSVYPKLKPGLVTDLRSVF 1366
            K LL++AL H SY       CYQRLEFLGD+VLDYLIT +LY  YP L PGL+TD+RS  
Sbjct: 1211 KSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLSPGLLTDMRSAS 1270

Query: 1367 VMNEAFANISVDRFFYKFLICDSTSLQKDIKSYVNFIK--SPPSERGSLEQPTCPKVLGD 1426
            V NE +A ++V    +K ++  S  L K I   V+  +  S  S  G     + PKVLGD
Sbjct: 1271 VNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWESDISFPKVLGD 1330

Query: 1427 MVESIVGAVLVDTGFDMNYVWKIMLSFLDSIMSFSGFQLSPIRDITEFCQDCGWKLQFNA 1486
            ++ES+ GA+ VD+G++   V+  +   L  +++    +L P+R++TE CQ   W+ + + 
Sbjct: 1331 VIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELCQK--WQFELSK 1369

Query: 1487 TKVEKYFSVKAEVKGNNFHETASAANRRKKDAEKIAANLILSKLK 1501
             K    F+V+ + K  +F  TA A++  KK A+K+A   +L+ LK
Sbjct: 1391 AKDFDSFTVEVKAKEMSFAHTAKASD--KKMAKKLAYKEVLNLLK 1369