Sed0002069 (gene) Chayote v1

BLAST of Sed0002069 vs. NCBI nr
Match: XP_038904203.1 (uncharacterized protein LOC120090550 [Benincasa hispida])

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 567/776 (73.07%), Postives = 650/776 (83.76%), Query Frame = 0

Query: 1   MEASNISPLLLLLLLFFSLLQTSTIAIKKSYIV--------ALDPSSNLSTHFQQVTAFH 60
           MEASN+SP LLL   +FSLLQTS IA KKSYIV        +LDPSS LS H +QVTAFH
Sbjct: 1   MEASNLSP-LLLFFFYFSLLQTSIIATKKSYIVYLGSERSSSLDPSS-LSEHSRQVTAFH 60

Query: 61  YDLLGSLLGSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHT 120
           YDLLG+LLGS+KM +EAIFYSYT SFNGFAAKLDEKEAA+LARNP VISV ENKARKLHT
Sbjct: 61  YDLLGTLLGSQKMTEEAIFYSYTTSFNGFAAKLDEKEAANLARNPKVISVFENKARKLHT 120

Query: 121 TRSWNFLGLEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWR 180
           TRSWNFLG+E + G IPSNS+W AA+FGQD+I+ NID+GVWPESKSFSDEGYG VPSKWR
Sbjct: 121 TRSWNFLGVENDVG-IPSNSIWNAAKFGQDIIIANIDTGVWPESKSFSDEGYGPVPSKWR 180

Query: 181 GICQTDSNFYCNKKLIGGRYFHKGYEAAGGVLDPSLLTVRDHDGHGTHTLSTAAGNFVAG 240
           GICQTDSNF+CN+KLIGGRYF+KGY AAGG L+ + LTVRDHDGHGTHTLSTAAGNFV G
Sbjct: 181 GICQTDSNFHCNRKLIGGRYFYKGYVAAGGTLNATSLTVRDHDGHGTHTLSTAAGNFVTG 240

Query: 241 ANVFGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISGSIG 300
           ANVFGHG+GTAKGGAPKARV AYKVCWPPF G  C DADI+AAFEAA+ADGVDVIS S+G
Sbjct: 241 ANVFGHGSGTAKGGAPKARVAAYKVCWPPFLGSQCLDADILAAFEAAVADGVDVISTSLG 300

Query: 301 ATAGEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRDFAS 360
             A E+FNDP+A+ +FHAVQQG+ VV S GN GP P TV+N+APW+ TVAA T+DRDFAS
Sbjct: 301 GAADEYFNDPLAIAAFHAVQQGVVVVFSAGNSGPFPMTVTNIAPWINTVAAGTVDRDFAS 360

Query: 361 YVVLGNKKILKGVSLSSVAPLPEKFYPLITAVDAKFSNITDLSAQSCGAGTLDPIKVKGK 420
            V LGNK   +GVSLSS+APLP+KFYPLI +V+AK SN+T+  A+ CG GTLDP+KVKGK
Sbjct: 361 NVALGNK---EGVSLSSIAPLPKKFYPLIDSVNAKLSNVTEFHARFCGEGTLDPMKVKGK 420

Query: 421 ILICVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGDAELLKQ 480
           I+IC  G ++ VEK Y AA+AGAVG+I+AN  EKGDEI+P+LHF+PASDIT+ DA+LL++
Sbjct: 421 IVICQVGEIEGVEKSYQAARAGAVGVIVANDIEKGDEIYPELHFIPASDITNNDAQLLQK 480

Query: 481 YLTSTKTPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDITAPGVTILASYPT 540
           YL ST TP AHL  VKT L +KPAP++A+FSSRGPN +   ILKPDITAPGV ILASY T
Sbjct: 481 YLNSTTTPMAHLTKVKTLLNIKPAPIIATFSSRGPNPIDPFILKPDITAPGVNILASYST 540

Query: 541 VTPPFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIMTTAKTLD 600
              P  S+ND RRIPFN +SGTSMSCPHI+GI  LLK IHP WSP+AIKSAIMTTAKT  
Sbjct: 541 GNAPTFSSNDQRRIPFNVISGTSMSCPHIAGIAGLLKSIHPDWSPAAIKSAIMTTAKTRG 600

Query: 601 NNLKTILDWTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGYNAMDIKKF 660
           NNL+TILD TKL+AT +AYG G V PN A DPGLVYD T EDYLNFLCARGYNAM++KKF
Sbjct: 601 NNLQTILDSTKLKATSYAYGAGMVHPNDARDPGLVYDTTIEDYLNFLCARGYNAMEMKKF 660

Query: 661 YDKPFTCDKSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTYVAQVKVSPQVSV 720
           Y KPF C KSF   DLNYPS S+G  L++GA VTINRR+K+VGSPGTYVA+VKVSP V+V
Sbjct: 661 YAKPFNCVKSFKATDLNYPSISVG-VLRIGAPVTINRRVKSVGSPGTYVARVKVSPGVAV 720

Query: 721 SVKPSMLQFSRLGEEKAFKVVVQNKGKVKHEGYVFGTLVWYDGKHSVKSPIAINLG 769
            V+P  LQFS +GEEKAFKVV+QN GKVK  G VFGTL+W DGKH V+SPIA++LG
Sbjct: 721 LVEPRTLQFSSVGEEKAFKVVLQNTGKVK-RGDVFGTLIWSDGKHFVRSPIAVHLG 768

BLAST of Sed0002069 vs. NCBI nr
Match: XP_031738719.1 (uncharacterized protein LOC101212014 [Cucumis sativus])

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 536/735 (72.93%), Postives = 621/735 (84.49%), Query Frame = 0

Query: 34   ALDPSSNLSTHFQQVTAFHYDLLGSLLGSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADL 93
            ALDPSS LS H +QVTA HYDLLGSL GSK MA+EAIFYSYTRSFNGFAAKLD+KEA +L
Sbjct: 808  ALDPSS-LSEHSRQVTALHYDLLGSLFGSKTMAEEAIFYSYTRSFNGFAAKLDDKEAENL 867

Query: 94   ARNPNVISVLENKARKLHTTRSWNFLGLEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVW 153
            ARNP VISV ENKARKLHTTRSWNFLG+E + G IPSNS+W  A+FG+D+IV NID+GVW
Sbjct: 868  ARNPKVISVFENKARKLHTTRSWNFLGVENDIG-IPSNSIWNTAKFGEDVIVANIDTGVW 927

Query: 154  PESKSFSDEGYGAVPSKWRGICQTDSNFYCNKKLIGGRYFHKGYEAAGGVLDPSLLTVRD 213
            PESKSFSDEGYG VPSKWRGICQTDS F+CN+KLIGGRYFHKGYEAAGG L+ +LLTVRD
Sbjct: 928  PESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRD 987

Query: 214  HDGHGTHTLSTAAGNFVAGANVFGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADII 273
            HDGHGTHTLSTAAGNFV GANVFGHGNGTAKGGAPKAR VAYK CWPP     CFDADI+
Sbjct: 988  HDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKACWPPLFDSQCFDADIL 1047

Query: 274  AAFEAAIADGVDVISGSIGATAGEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSN 333
            AAFEAAIADGVDV+S S+G  A E+FNDP+A+ +F AVQ+GI VV SGGN GP P T++N
Sbjct: 1048 AAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTIAN 1107

Query: 334  VAPWVTTVAASTMDRDFASYVVLGNKKILKGVSLSSVAPLPEKFYPLITAVDAKFSNITD 393
            ++PWV TVAAST+DR+FASYV LGNKK +KG+SLSSV  LP+KF+PLI +VDAKF N+T+
Sbjct: 1108 ISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLSSVPSLPKKFFPLINSVDAKFRNVTE 1167

Query: 394  LSAQSCGAGTLDPIKVKGKILICVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQ 453
              AQ CG GTLDP+KVKGKI+IC  G    V+KG+ A++AGAVG+I+AN  EKGDEIFP+
Sbjct: 1168 FHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGFQASRAGAVGVIIANDLEKGDEIFPE 1227

Query: 454  LHFLPASDITSGDAELLKQYLTSTKTPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSN 513
            LHF+PASDIT+ DA++++ YL ST+TP AHL +VKT L VKPAP +A+FS+RGPN + S 
Sbjct: 1228 LHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVKTLLSVKPAPTIATFSARGPNPIDST 1287

Query: 514  ILKPDITAPGVTILASYPTVTPPFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHP 573
            ILKPD+TAPGV ILASYPT   P  S  D RRIPFN +SGTSMSCPH++GI  L+K IHP
Sbjct: 1288 ILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPFNVISGTSMSCPHVAGIAGLIKSIHP 1347

Query: 574  KWSPSAIKSAIMTTAKTLDNNLKTILDWTKLEATPFAYGGGHVDPNAAMDPGLVYDATFE 633
             WSP+AIKSAIMTTAKT  NN +TILD TKL+ATP+AYG G V+PN A DPGLVYD T  
Sbjct: 1348 NWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVNPNDAADPGLVYDITVN 1407

Query: 634  DYLNFLCARGYNAMDIKKFYDKPFTCDKSFHVRDLNYPSFSIGEALKVGASVTINRRLKN 693
            DYLNFLCARGYNAM IKKFY KPF+C +SF V DLNYPS S+GE LK+GA +T+NRR+KN
Sbjct: 1408 DYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDLNYPSISVGE-LKIGAPLTMNRRVKN 1467

Query: 694  VGSPGTYVAQVKVSPQVSVSVKPSMLQFSRLGEEKAFKVVVQNKGKVKHEGYVFGTLVWY 753
            VGSPGTYVA+VK SP V+VS++PS L FSR+GEEK FKVV+QN GKVK+   VFGTL+W 
Sbjct: 1468 VGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKGFKVVLQNTGKVKNGSDVFGTLIWS 1527

Query: 754  DGKHSVKSPIAINLG 769
            DGKH V+S IA++LG
Sbjct: 1528 DGKHFVRSSIAVHLG 1539

BLAST of Sed0002069 vs. NCBI nr
Match: XP_016900593.1 (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo])

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 558/779 (71.63%), Postives = 639/779 (82.03%), Query Frame = 0

Query: 6   ISPLLLLLLLFFSL------LQTSTI--AIKKSYIV--------ALDPSSNLSTHFQQVT 65
           IS L + LLLF S       L  STI      SYIV        +LDPSS LS H QQVT
Sbjct: 74  ISNLKMALLLFESASGLNINLNKSTIFPINVPSYIVYLGSEHSSSLDPSS-LSEHSQQVT 133

Query: 66  AFHYDLLGSLLGSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARK 125
           A HYDLLGS LGSK MA+EAIFYSYTRSFNGFAA LD+KEA +LARNP VISV ENKARK
Sbjct: 134 ALHYDLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARK 193

Query: 126 LHTTRSWNFLGLEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPS 185
           LHTTRSWNFLG+E + G IPSNS+W AA+FG+D+I+ NID+GVWPESKSFSDEGYG VPS
Sbjct: 194 LHTTRSWNFLGVENDIG-IPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPS 253

Query: 186 KWRGICQTDSNFYCNKKLIGGRYFHKGYEAAGGVLDPSLLTVRDHDGHGTHTLSTAAGNF 245
           KWRGICQTDSNF+CN+KLIGGRYFHKGYEAAGG L+ +LLTVRDHDGHGTHTLSTAAGNF
Sbjct: 254 KWRGICQTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNF 313

Query: 246 VAGANVFGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISG 305
           V GANVFGHGNGTAKGGAPKAR VAYKVCWPP     CFDADI+AAFEAAIADGVDV+S 
Sbjct: 314 VTGANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLST 373

Query: 306 SIGATAGEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRD 365
           S+G  A E+FNDP+A+ +F AVQ+GI VV SGGN GP P TV+NV+PWV TVAA+T+DRD
Sbjct: 374 SLGGAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRD 433

Query: 366 FASYVVLGNKKILKGVSLSSVAPLPEKFYPLITAVDAKFSNITDLSAQSCGAGTLDPIKV 425
           F SYV LGNKK +KGVSLSSVA LP+KFYPLI +VDAKFSN+T+  AQ CG GTLDP+KV
Sbjct: 434 FVSYVGLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKV 493

Query: 426 KGKILICVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGDAEL 485
           KGKI+IC  G  + V+KGY A+ AGA G+I+AN  EKGDEI+P+LHF+PASDIT+ DA++
Sbjct: 494 KGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQI 553

Query: 486 LKQYLTSTKTPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDITAPGVTILAS 545
           +++YL STKTP AHL +VKT L VKPAP++A+FSSRGPN + S ILKPD+TAPGV ILAS
Sbjct: 554 VQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILAS 613

Query: 546 YPTVTPPFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIMTTAK 605
           YPT   P  S  D RRIPFN +SGTSMSCPH++GI  L+K IHP WSP+AIKSAIMTTAK
Sbjct: 614 YPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAK 673

Query: 606 TLDNNLKTILDWTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGYNAMDI 665
           T  NN +TILD TKL+ATP+AYG G V PN A DPGLVYD T  DYLNFLCARGY+AM I
Sbjct: 674 TRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKI 733

Query: 666 KKFYDKPFTCDKSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTYVAQVKVSPQ 725
           KKFY KPF C +SF V DLNYPS S+GE LK+GA +TINRR+KNVGSPGTYVA+VK SP 
Sbjct: 734 KKFYAKPFNCVRSFKVTDLNYPSISVGE-LKIGAPLTINRRVKNVGSPGTYVARVKASPG 793

Query: 726 VSVSVKPSMLQFSRLGEEKAFKVVVQNKGKVKHEGYVFGTLVWYDGKHSVKSPIAINLG 769
           V+V+++PS L F  +GEEK FKVV+QN GKVK  G VFGTL+W DGKH V+SPIA++LG
Sbjct: 794 VAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVK-SGDVFGTLIWSDGKHFVRSPIAVHLG 848

BLAST of Sed0002069 vs. NCBI nr
Match: KAE8650095.1 (hypothetical protein Csa_011146 [Cucumis sativus])

HSP 1 Score: 1073.2 bits (2774), Expect = 1.2e-309
Identity = 531/744 (71.37%), Postives = 618/744 (83.06%), Query Frame = 0

Query: 20  LQTSTIAIKKSYIVALDPSSNLSTHFQQVTAFHYDLLGSLLGSKKMAQEAIFYSYTRSFN 79
           +Q+  + +   +  ALDPSS LS H +QVTA HYDLLGSL GSK MA+EAIFYSYTRSFN
Sbjct: 1   MQSYIVYLGSEHSSALDPSS-LSEHSRQVTALHYDLLGSLFGSKTMAEEAIFYSYTRSFN 60

Query: 80  GFAAKLDEKEAADLARNPNVISVLENKARKLHTTRSWNFLGLEKEQGNIPSNSLWKAAEF 139
           GFAAKLD+KEA +LARNP VISV ENKARKLHTTRSWNFLG+E + G IPSNS+W  A+F
Sbjct: 61  GFAAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIG-IPSNSIWNTAKF 120

Query: 140 GQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWRGICQTDSNFYCNKKLIGGRYFHKGYEA 199
           G+D+IV NID+GVWPESKSFSDEGYG VPSKWRGICQTDS F+CN+KLIGGRYFHKGYEA
Sbjct: 121 GEDVIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLIGGRYFHKGYEA 180

Query: 200 AGGVLDPSLLTVRDHDGHGTHTLSTAAGNFVAGANVFGHGNGTAKGGAPKARVVAYKVCW 259
           AGG L+ +LLTVRDHDGHGTHTLSTAAGNFV GANVFGHGNGTAKGGAPKAR VAYK CW
Sbjct: 181 AGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKACW 240

Query: 260 PPFNGGMCFDADIIAAFEAAIADGVDVISGSIGATAGEFFNDPIAMISFHAVQQGIDVVL 319
           PP     CFDADI+AAFEAAIADGVDV+S S+G  A E+FNDP+A+ +F AVQ+GI VV 
Sbjct: 241 PPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVF 300

Query: 320 SGGNEGPLPGTVSNVAPWVTTVAASTMDRDFASYVVLGNKKILKGVSLSSVAPLPEKFYP 379
           SGGN GP P T++N++PWV TVAAST+DR+FASYV LGNKK +KG+SLSSV  LP+KF+P
Sbjct: 301 SGGNSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLSSVPSLPKKFFP 360

Query: 380 LITAVDAKFSNITDLSAQSCGAGTLDPIKVKGKILICVAGGLQPVEKGYHAAQAGAVGMI 439
           LI +VDAKF N+T+  AQ CG GTLDP+KVKGKI+IC  G    V+KG+ A++AGAVG+I
Sbjct: 361 LINSVDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGFQASRAGAVGVI 420

Query: 440 LANLNEKGDEIFPQLHFLPASDITSGDAELLKQYLTSTKTPEAHLKNVKTELGVKPAPVM 499
           +AN  EKGDEIFP+LHF+PASDIT+ DA++++ YL ST+TP AHL +VKT L VKPAP +
Sbjct: 421 IANDLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVKTLLSVKPAPTI 480

Query: 500 ASFSSRGPNLVVSNILKPDITAPGVTILASYPTVTPPFPSTNDHRRIPFNALSGTSMSCP 559
           A+FS+RGPN + S ILKPD+TAPGV ILASYPT   P  S  D RRIPFN +SGTSMSCP
Sbjct: 481 ATFSARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPFNVISGTSMSCP 540

Query: 560 HISGIVALLKFIHPKWSPSAIKSAIMTT------AKTLDNNLKTILDWTKLEATPFAYGG 619
           H++GI  L+K IHP WSP+AIKSAIMTT      AKT  NN +TILD TKL+ATP+AYG 
Sbjct: 541 HVAGIAGLIKSIHPNWSPAAIKSAIMTTVTYSFAAKTRGNNNQTILDSTKLKATPYAYGA 600

Query: 620 GHVDPNAAMDPGLVYDATFEDYLNFLCARGYNAMDIKKFYDKPFTCDKSFHVRDLNYPSF 679
           G V+PN A DPGLVYD T  DYLNFLCARGYNAM IKKFY KPF+C +SF V DLNYPS 
Sbjct: 601 GQVNPNDAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDLNYPSI 660

Query: 680 SIGEALKVGASVTINRRLKNVGSPGTYVAQVKVSPQVSVSVKPSMLQFSRLGEEKAFKVV 739
           S+GE LK+GA +T+NRR+KNVGSPGTYVA+VK SP V+VS++PS L FSR+GEEK FKVV
Sbjct: 661 SVGE-LKIGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKGFKVV 720

Query: 740 VQNKGKVKHEGYVFGTLVWYDGKH 758
           +QN GKVK+   VFGTL+W DGKH
Sbjct: 721 LQNTGKVKNGSDVFGTLIWSDGKH 741

BLAST of Sed0002069 vs. NCBI nr
Match: TYK19384.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])

HSP 1 Score: 1067.4 bits (2759), Expect = 5.8e-308
Identity = 550/788 (69.80%), Postives = 631/788 (80.08%), Query Frame = 0

Query: 1   MEASNISPLLLLLLLFFSLLQTSTIAIKKSYIV--------ALDPSSNLSTHFQQVTAFH 60
           MEASN+SP LLL   +FSLLQTSTIA KKSYIV        +LDPSS LS H QQVTA H
Sbjct: 1   MEASNLSP-LLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSS-LSEHSQQVTALH 60

Query: 61  YDLLGSLLGSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHT 120
           YDLLGS LGSK MA+EAIFYSYTRSFNGFAA LD+KEA +LARNP VISV ENKARKLHT
Sbjct: 61  YDLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHT 120

Query: 121 TRSWNFLGLEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWR 180
           TRSWNFLG+E + G IPSNS+W AA+FG+D+I+ NID+GVWPESKSFSDEGYG VPSKWR
Sbjct: 121 TRSWNFLGVENDIG-IPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWR 180

Query: 181 GICQTDSNFYCNKKLIGGRYFHKGYEAAGGVLDPSLLTVRDHDGHGTHTLSTAAGNFVAG 240
           GICQTDSNF CN+KLIGGRYFHKGYEAAGG L+ +LLTVRDHDGHGTHTLSTAAGNFV G
Sbjct: 181 GICQTDSNFLCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTG 240

Query: 241 ANVFGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISGSIG 300
           ANVFGHGNGTAKGGAPKAR VAYKVCWPP     CFDADI+AAFEAAIADGVDV+S S+G
Sbjct: 241 ANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLG 300

Query: 301 ATAGEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRDFAS 360
             A E+FNDP+A+ +F AVQ+GI VV SGGN GP P TV+NV+PWV TVAA+T+DRDF S
Sbjct: 301 GAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVS 360

Query: 361 YVVLGNKKILK--------GVSLSSVAP----LPEKFYPLITAVDAKFSNITDLSAQSCG 420
           YV LGNKK +K        G+  S  AP     PE +            +  + S Q CG
Sbjct: 361 YVGLGNKKHIKVHVYTHLLGLGASRFAPNFICAPELYN------QYSIGSTLETSNQFCG 420

Query: 421 AGTLDPIKVKGKILICVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPAS 480
            GTLDP+KVKGKI+IC  G  + V+KGY A+ AGA G+I+AN  EKGDEI+P+LHF+PAS
Sbjct: 421 KGTLDPMKVKGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPAS 480

Query: 481 DITSGDAELLKQYLTSTKTPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDIT 540
           DIT+ DA+++++YL STKTP AHL +VKT L VKPAP++A+FSSRGPN + S ILKPD+T
Sbjct: 481 DITNTDAQIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVT 540

Query: 541 APGVTILASYPTVTPPFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAI 600
           APGV ILASYPT   P  S  D RRIPFN +SGTSMSCPH++GI  L+K IHP WSP+AI
Sbjct: 541 APGVNILASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAI 600

Query: 601 KSAIMTTAKTLDNNLKTILDWTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLC 660
           KSAIMTTAKT  NN +TILD TKL+ATP+AYG G V PN A DPGLVYD T  DYLNFLC
Sbjct: 601 KSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLC 660

Query: 661 ARGYNAMDIKKFYDKPFTCDKSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTY 720
           ARGY+AM IKKFY KPF C +SF V DLNYPS S+GE LK+GA +TINRR+KNVGSPGTY
Sbjct: 661 ARGYDAMKIKKFYAKPFNCVRSFKVTDLNYPSISVGE-LKIGAPLTINRRVKNVGSPGTY 720

Query: 721 VAQVKVSPQVSVSVKPSMLQFSRLGEEKAFKVVVQNKGKVKHEGYVFGTLVWYDGKHSVK 769
           VA+VK SP V+V+++PS L F  +GEEK FKVV+QN GKVK  G VFGTL+W DGKH V+
Sbjct: 721 VARVKASPGVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVK-SGDVFGTLIWSDGKHFVR 777

BLAST of Sed0002069 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 2.5e-229
Identity = 413/771 (53.57%), Postives = 531/771 (68.87%), Query Frame = 0

Query: 5   NISPLLLLLLLFFSLLQTSTIAIKKSYIVALDPSSNL----STHFQQVTAFHYDLLGSLL 64
           N S LLLLLL+  S           SY+V     S++         +V   HYD LGS  
Sbjct: 6   NFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFT 65

Query: 65  GSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHTTRSWNFLG 124
           GS++ A +AIFYSYT+  NGFAA LD   A +++++P V+SV  NKA KLHTTRSW+FLG
Sbjct: 66  GSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLG 125

Query: 125 LEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWRGICQT--D 184
           LE     +PS+S+W+ A FG+D I+ N+D+GVWPESKSF DEG G +PS+W+GICQ   D
Sbjct: 126 LE-HNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKD 185

Query: 185 SNFYCNKKLIGGRYFHKGYEAAGGVLDPSLLTVRDHDGHGTHTLSTAAGNFVAGANVFGH 244
           + F+CN+KLIG RYF+KGY AA G L+ S  + RD DGHG+HTLSTAAG+FV G ++FG 
Sbjct: 186 ATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQ 245

Query: 245 GNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISGSIGATAGEF 304
           GNGTAKGG+P+ARV AYKVCWPP  G  C+DAD++AAF+AAI DG DVIS S+G     F
Sbjct: 246 GNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSF 305

Query: 305 FNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRDFASYVVLGN 364
           FND +A+ SFHA ++ I VV S GN GP   TVSNVAPW  TV ASTMDR+FAS +VLGN
Sbjct: 306 FNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGN 365

Query: 365 KKILKGVSLSSVAPLPEKFYPLITAVDAKFSNITDLSAQSCGAGTLDPIKVKGKILICVA 424
            K  KG SLSS A    KFYP++ +V+AK  N + L AQ C  G+LDPIK KGKIL+C+ 
Sbjct: 366 GKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLR 425

Query: 425 GGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGDAELLKQYLTSTK 484
           G    VEKG   A  G +GM+L N    G+++    H LPA+ +TS D+  + +Y++ TK
Sbjct: 426 GQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTK 485

Query: 485 TPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDITAPGVTILASYPTVTPPFP 544
            P AH+   +T+LG+KPAPVMASFSS+GP++V   ILKPDITAPGV+++A+Y     P  
Sbjct: 486 KPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTN 545

Query: 545 STNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIMTTAKTLDNNLKTI 604
              D RR+ FNA+SGTSMSCPHISGI  LLK  +P WSP+AI+SAIMTTA  +D+    I
Sbjct: 546 EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPI 605

Query: 605 LDWTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGYNAMDIKKFYDKPFT 664
            + T ++ATPF++G GHV PN A++PGLVYD   +DYLNFLC+ GYNA  I  F    FT
Sbjct: 606 QNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFT 665

Query: 665 CDK-SFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTYVAQVKVSPQVSVSVKPS 724
           C      + +LNYPS ++       + VT++R +KNVG P  Y  +V     V V+VKP+
Sbjct: 666 CSSPKISLVNLNYPSITVPNL--TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPT 725

Query: 725 MLQFSRLGEEKAFKVV-VQNKGKVKHEGYVFGTLVWYDGKHSVKSPIAINL 768
            L F+++GE+K FKV+ V++KG V  +GYVFG LVW D KH V+SPI + L
Sbjct: 726 SLNFTKVGEQKTFKVILVKSKGNVA-KGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Sed0002069 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 2.5e-229
Identity = 425/774 (54.91%), Postives = 527/774 (68.09%), Query Frame = 0

Query: 1   MEASNISPLLLLLLLFFSLLQTSTIAIKKSYIVALDPSSNL----STHFQQVTAFHYDLL 60
           M   ++S LLLL+ LFFS       A+KKSYIV L   ++L    S H   V   H   L
Sbjct: 16  MSLQSLSSLLLLVTLFFS----PAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFL 75

Query: 61  GSLLGSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHTTRSW 120
            S +GS + A+EAIFYSY R  NGFAA LDE EAA++A++P+V+SV  NK RKLHTT SW
Sbjct: 76  ASFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSW 135

Query: 121 NFLGLEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWRGICQ 180
           NF+ L K  G +  +SLW  A +G+D I+ N+D+GVWPESKSFSDEGYGAVP++W+G C 
Sbjct: 136 NFMLLAK-NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH 195

Query: 181 TDSNFYCNKKLIGGRYFHKGYEAAGGV-LDPSLLTVRDHDGHGTHTLSTAAGNFVAGANV 240
            D    CN+KLIG RYF+KGY A  G+  + S  T RDHDGHG+HTLSTAAGNFV GANV
Sbjct: 196 KD--VPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANV 255

Query: 241 FGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISGSIGATA 300
           FG GNGTA GG+PKARV AYKVCWPP +G  CFDADI+AA EAAI DGVDV+S S+G  A
Sbjct: 256 FGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDA 315

Query: 301 GEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRDFASYVV 360
           G++ +D IA+ SFHAV+ G+ VV S GN GP  GTVSNVAPWV TV AS+MDR+F ++V 
Sbjct: 316 GDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVE 375

Query: 361 LGNKKILKGVSLSSVAPLP-EKFYPLITAVDAKFSNITDLSAQSCGAGTLDPIKVKGKIL 420
           L N +  KG SLS   PLP EK Y LI+A DA  +N     A  C  G+LDP KVKGKIL
Sbjct: 376 LKNGQSFKGTSLSK--PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKIL 435

Query: 421 ICVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGDAELLKQYL 480
           +C+ G    V+KG  AA AGA GM+L N    G+EI    H LPAS I   D E L  YL
Sbjct: 436 VCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYL 495

Query: 481 TSTKTPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDITAPGVTILASYPTVT 540
           +STK P+ ++K     L  KPAP MASFSSRGPN +   ILKPDITAPGV I+A++   T
Sbjct: 496 SSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEAT 555

Query: 541 PPFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIMTTAKTLDNN 600
            P    +D+RR PFN  SGTSMSCPHISG+V LLK +HP WSP+AI+SAIMTT++T +N 
Sbjct: 556 GPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNR 615

Query: 601 LKTILDWTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGYNAMDIKKFYD 660
            K ++D +  +A PF+YG GHV PN A  PGLVYD T  DYL+FLCA GYN   ++ F +
Sbjct: 616 RKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAE 675

Query: 661 KP-FTCDKSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTYVAQVKVSPQVSVS 720
            P +TC +  ++ D NYPS ++     +  S+T+ R+LKNVG P TY A+ +    V VS
Sbjct: 676 DPQYTCRQGANLLDFNYPSITVP---NLTGSITVTRKLKNVGPPATYNARFREPLGVRVS 735

Query: 721 VKPSMLQFSRLGEEKAFKVVVQNKGKVKHEGYVFGTLVWYDGKHSVKSPIAINL 768
           V+P  L F++ GE K F++ ++    V   GYVFG L W D  H V+SPI + L
Sbjct: 736 VEPKQLTFNKTGEVKIFQMTLRPL-PVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Sed0002069 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 685.3 bits (1767), Expect = 8.2e-196
Identity = 388/785 (49.43%), Postives = 492/785 (62.68%), Query Frame = 0

Query: 9   LLLLLLLFFSLLQTSTIAIKKSYIVALDPSSN----LSTHFQQVTAFHYDLLGSLLGSKK 68
           L+L     F+ L  +    KK YIV +   S+     S   +  T  HYDLLGS+ GS++
Sbjct: 10  LILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSRE 69

Query: 69  MAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHTTRSWNFLGLEKE 128
            A+EAI YSY R  NGFAA L+E+EAAD+A+NPNV+SV  +K  KLHTTRSW FLGL + 
Sbjct: 70  KAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRR 129

Query: 129 QGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWR-GICQTDS---- 188
                 NS W+   FG++ I+GNID+GVWPES+SFSD+GYG VPSKWR G+CQ +     
Sbjct: 130 ----GQNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGS 189

Query: 189 -NFYCNKKLIGGRYFHKGYEAAGGVLDPSLLTVRDHDGHGTHTLSTAAGNFVAGANVFGH 248
               CN+KLIG RY++K +EA  G LDP L T RD  GHGTHTLSTA GNFV GA VF  
Sbjct: 190 MKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAV 249

Query: 249 GNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISGSIG----AT 308
           GNGTAKGG+P+ARV AYKVCW   +   C+ AD++AA + AI DGVDVI+ S G     T
Sbjct: 250 GNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVT 309

Query: 309 AGEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRDFASYV 368
           A   F D I++ +FHA+ + I +V S GN+GP PGTV+NVAPWV T+AAST+DRDF+S +
Sbjct: 310 AEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNL 369

Query: 369 VLGNKKILKGVSLSSVAPLPEKFYPLITAVDAKFSNITDLSAQSCGAGTLDPIKVKGKIL 428
            + N ++++G SL    P P + + LI + DAK +N T   AQ C  GTLD  KV GKI+
Sbjct: 370 TI-NNQLIEGASLFVNLP-PNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIV 429

Query: 429 ICV-AGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGDAELLKQY 488
           +C   G ++ V +G  A  AGA GMIL N  + G  +  + H     +     A+     
Sbjct: 430 LCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHG 489

Query: 489 LTST---------KTPEA-HLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDITAPG 548
           + +T         KT +   +   +T  G KPAPVMASFSSRGPN +  +ILKPD+TAPG
Sbjct: 490 VKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPG 549

Query: 549 VTILASYPTVTPPFPSTNDHRR-IPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKS 608
           V ILA+Y           D+RR   FN L GTSMSCPH SGI  LLK  HP WSP+AIKS
Sbjct: 550 VNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKS 609

Query: 609 AIMTTAKTLDNNLKTILD-WTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCA 668
           AIMTTA TLDN  + I D + K  A  FAYG GHV P+ A++PGLVYD +  DYLNFLCA
Sbjct: 610 AIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCA 669

Query: 669 RGYNAMDIKKF-YDKPFTCDKSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTY 728
            GY+   I    +++ F C  S  V DLNYPS ++   L++   VTI R + NVG P TY
Sbjct: 670 SGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPN-LRL-KPVTIARTVTNVGPPSTY 729

Query: 729 VAQVKVSPQVSVSVKPSMLQFSRLGEEKAFKVVVQNKGKVKHEGYVFGTLVWYDGKHSVK 766
               +     S++V P  L F+++GE K FKV+VQ         Y FG L W DGKH V+
Sbjct: 730 TVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVR 786

BLAST of Sed0002069 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.7e-169
Identity = 340/777 (43.76%), Postives = 475/777 (61.13%), Query Frame = 0

Query: 1   MEASNISPLLLLLLLFFSLLQTSTIAIKKSYIVALDPSSNLSTHFQQVTAFHYDLLGSLL 60
           M +S +S     LLL       S+ +  +   +     S + + F   + ++   L S+ 
Sbjct: 1   MSSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSIS 60

Query: 61  GSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHTTRSWNFLG 120
            S +     + Y+Y  + +GF+ +L ++EA  L   P VISVL     +LHTTR+  FLG
Sbjct: 61  DSAE-----LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLG 120

Query: 121 LEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWRGICQTDSN 180
           L++   +     L+  A    D++VG +D+GVWPESKS+SDEG+G +PS W+G C+  +N
Sbjct: 121 LDEHTAD-----LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTN 180

Query: 181 F---YCNKKLIGGRYFHKGYEAAGGVLDPS--LLTVRDHDGHGTHTLSTAAGNFVAGANV 240
           F    CN+KLIG R+F +GYE+  G +D S    + RD DGHGTHT STAAG+ V GA++
Sbjct: 181 FTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASL 240

Query: 241 FGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISGSIGATA 300
            G+ +GTA+G AP+ARV  YKVCW     G CF +DI+AA + AIAD V+V+S S+G   
Sbjct: 241 LGYASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGM 300

Query: 301 GEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRDFASYVV 360
            +++ D +A+ +F A+++GI V  S GN GP   ++SNVAPW+TTV A T+DRDF +  +
Sbjct: 301 SDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAI 360

Query: 361 LGNKKILKGVSLSSVAPLPEKFYPLITAVDAKFSNITDLSAQSCGAGTLDPIKVKGKILI 420
           LGN K   GVSL     LP+K  P I A +A  SN T  +   C  GTL P KVKGKI++
Sbjct: 361 LGNGKNFTGVSLFKGEALPDKLLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKIVM 420

Query: 421 CVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGDAELLKQYLT 480
           C  G    V+KG     AG VGMILAN    G+E+    H LPA+ +     ++++ Y+T
Sbjct: 421 CDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVT 480

Query: 481 STKTPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDITAPGVTILASYPTVTP 540
           +   P A +  + T +GVKP+PV+A+FSSRGPN +  NILKPD+ APGV ILA++     
Sbjct: 481 TDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAG 540

Query: 541 PFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIMTTAKTLDNNL 600
           P    +D RR+ FN +SGTSMSCPH+SG+ ALLK +HP+WSP+AI+SA+MTTA     + 
Sbjct: 541 PTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDG 600

Query: 601 KTILD-WTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGYNAMDIKKFYD 660
           K +LD  T   +TPF +G GHV P  A +PGL+YD T EDYL FLCA  Y +  I+    
Sbjct: 601 KPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR 660

Query: 661 KPFTCD--KSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTYVAQVKVSPQ--- 720
           + +TCD  KS+ V DLNYPSF++     VGA     R + +VG  GTY   VKV+ +   
Sbjct: 661 RNYTCDPSKSYSVADLNYPSFAV-NVDGVGA-YKYTRTVTSVGGAGTY--SVKVTSETTG 720

Query: 721 VSVSVKPSMLQFSRLGEEKAFKVVVQNKGKVKHEGYVFGTLVWYDGKHSVKSPIAIN 767
           V +SV+P++L F    E+K++ V              FG++ W DGKH V SP+AI+
Sbjct: 721 VKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Sed0002069 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 4.8e-156
Identity = 337/795 (42.39%), Postives = 467/795 (58.74%), Query Frame = 0

Query: 1   MEASNISPLLLLLLLFFSLLQTSTIAIKKSYIVALDPSSNLSTHFQQVTAFHYDLLGSLL 60
           +  S+I  +  LLL FFS   +S+  + +SYIV +  S   S  F     +H  LL SL 
Sbjct: 4   LSLSSIFFVFPLLLCFFSPSSSSSDGL-ESYIVHVQRSHKPSL-FSSHNNWHVSLLRSLP 63

Query: 61  GSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHTTRSWNFLG 120
            S + A   + YSY+R+ +GF+A+L   + A L R+P+VISV+ ++AR++HTT +  FLG
Sbjct: 64  SSPQPA--TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLG 123

Query: 121 LEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWRGICQTDSN 180
             +  G      LW  + +G+D+IVG +D+G+WPE  SFSD G G +PS W+G C+   +
Sbjct: 124 FSQNSG------LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPD 183

Query: 181 F---YCNKKLIGGRYFHKGY---------EAAGGVLDPSLLTVRDHDGHGTHTLSTAAGN 240
           F    CN+KLIG R F++GY          AA     P     RD +GHGTHT STAAG+
Sbjct: 184 FPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSP-----RDTEGHGTHTASTAAGS 243

Query: 241 FVAGANVFGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVIS 300
            VA A+++ +  GTA G A KAR+ AYK+CW     G C+D+DI+AA + A+ADGV VIS
Sbjct: 244 VVANASLYQYARGTATGMASKARIAAYKICWT----GGCYDSDILAAMDQAVADGVHVIS 303

Query: 301 GSIGA--TAGEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTM 360
            S+GA  +A E+  D IA+ +F A + GI V  S GN GP P T +N+APW+ TV AST+
Sbjct: 304 LSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTV 363

Query: 361 DRDFASYVVLGNKKILKGVSLSSVAPLPEKFYPLITAVDAKFSNITDLSAQSCGAGTLDP 420
           DR+FA+  + G+ K+  G SL +   LP+    L+ +         D  ++ C  G L+ 
Sbjct: 364 DREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVYS--------GDCGSRLCYPGKLNS 423

Query: 421 IKVKGKILICVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGD 480
             V+GKI++C  GG   VEKG     AG  GMILAN  E G+E+    H +PA+ + +  
Sbjct: 424 SLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKA 483

Query: 481 AELLKQYLTSTKTPEAHLKNVKTELGVK-PAPVMASFSSRGPNLVVSNILKPDITAPGVT 540
            + ++ Y+ ++ +P A +  + T +G   P+P +A+FSSRGPN +   ILKPD+ APGV 
Sbjct: 484 GDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVN 543

Query: 541 ILASYPTVTPPFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIM 600
           ILA +  +  P     D RR+ FN +SGTSMSCPH+SG+ ALL+  HP WSP+AIKSA++
Sbjct: 544 ILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALV 603

Query: 601 TTAKTLDNNLKTILDW-TKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGY 660
           TTA  ++N+ + I D  T   +  F +G GHVDPN A++PGLVYD   ++Y+ FLCA GY
Sbjct: 604 TTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGY 663

Query: 661 NAMDIKKFYDKPFTCD-----KSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGS--P 720
               I  F   P   D     K     DLNYPSFS+  A   G  V   R +KNVGS   
Sbjct: 664 EFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFA-STGEVVKYKRVVKNVGSNVD 723

Query: 721 GTYVAQVKVSPQVSVSVKPSMLQFSR----LGEEKAFKVVVQNKGKVKHEGYVFGTLVWY 769
             Y   VK    V + V PS L FS+    L  E  FK VV   G     G+ FG++ W 
Sbjct: 724 AVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWT 770

BLAST of Sed0002069 vs. ExPASy TrEMBL
Match: A0A0A0L241 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045090 PE=3 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 537/749 (71.70%), Postives = 627/749 (83.71%), Query Frame = 0

Query: 20  LQTSTIAIKKSYIVALDPSSNLSTHFQQVTAFHYDLLGSLLGSKKMAQEAIFYSYTRSFN 79
           +Q+  + +   +  ALDPSS LS H +QVTA HYDLLGSL GSK MA+EAIFYSYTRSFN
Sbjct: 1   MQSYIVYLGSEHSSALDPSS-LSEHSRQVTALHYDLLGSLFGSKTMAEEAIFYSYTRSFN 60

Query: 80  GFAAKLDEKEAADLARNPNVISVLENKARKLHTTRSWNFLGLEKEQGNIPSNSLWKAAEF 139
           GFAAKLD+KEA +LARNP VISV ENKARKLHTTRSWNFLG+E + G IPSNS+W  A+F
Sbjct: 61  GFAAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIG-IPSNSIWNTAKF 120

Query: 140 GQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWRGICQTDSNFYCNKKLIGGRYFHKGYEA 199
           G+D+IV NID+GVWPESKSFSDEGYG VPSKWRGICQTDS F+CN+KLIGGRYFHKGYEA
Sbjct: 121 GEDVIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLIGGRYFHKGYEA 180

Query: 200 AGGVLDPSLLTVRDHDGHGTHTLSTAAGNFVAGANVFGHGNGTAKGGAPKARVVAYKVCW 259
           AGG L+ +LLTVRDHDGHGTHTLSTAAGNFV GANVFGHGNGTAKGGAPKAR VAYK CW
Sbjct: 181 AGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKACW 240

Query: 260 PPFNGGMCFDADIIAAFEAAIADGVDVISGSIGATAGEFFNDPIAMISFHAVQQGIDVVL 319
           PP     CFDADI+AAFEAAIADGVDV+S S+G  A E+FNDP+A+ +F AVQ+GI VV 
Sbjct: 241 PPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVF 300

Query: 320 SGGNEGPLPGTVSNVAPWVTTVAASTMDRDFASYVVLGNKKILKGVSLSSVAPLPEKFYP 379
           SGGN GP P T++N++PWV TVAAST+DR+FASYV LGNKK +KG+SLSSV  LP+KF+P
Sbjct: 301 SGGNSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLSSVPSLPKKFFP 360

Query: 380 LITAVDAKFSNITDLSAQSCGAGTLDPIKVKGKILICVAGGLQPVEKGYHAAQAGAVGMI 439
           LI +VDAKF N+T+  AQ CG GTLDP+KVKGKI+IC  G    V+KG+ A++AGAVG+I
Sbjct: 361 LINSVDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGFQASRAGAVGVI 420

Query: 440 LANLNEKGDEIFPQLHFLPASDITSGDAELLKQYLTSTKTPEAHLKNVKTELGVKPAPVM 499
           +AN  EKGDEIFP+LHF+PASDIT+ DA++++ YL ST+TP AHL +VKT L VKPAP +
Sbjct: 421 IANDLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVKTLLSVKPAPTI 480

Query: 500 ASFSSRGPNLVVSNILKPDITAPGVTILASYPTVTPPFPSTNDHRRIPFNALSGTSMSCP 559
           A+FS+RGPN + S ILKPD+TAPGV ILASYPT   P  S  D RRIPFN +SGTSMSCP
Sbjct: 481 ATFSARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPFNVISGTSMSCP 540

Query: 560 HISGIVALLKFIHPKWSPSAIKSAIMTTAKTLDNNLKTILDWTKLEATPFAYGGGHVDPN 619
           H++GI  L+K IHP WSP+AIKSAIMTTAKT  NN +TILD TKL+ATP+AYG G V+PN
Sbjct: 541 HVAGIAGLIKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVNPN 600

Query: 620 AAMDPGLVYDATFEDYLNFLCARGYNAMDIKKFYDKPFTCDKSFHVRDLNYPSFSIGEAL 679
            A DPGLVYD T  DYLNFLCARGYNAM IKKFY KPF+C +SF V DLNYPS S+GE L
Sbjct: 601 DAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDLNYPSISVGE-L 660

Query: 680 KVGASVTINRRLKNVGSPGTYVAQVKVSPQVSVSVKPSMLQFSRLGEEKAFKVVVQNKGK 739
           K+GA +T+NRR+KNVGSPGTYVA+VK SP V+VS++PS L FSR+GEEK FKVV+QN GK
Sbjct: 661 KIGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKGFKVVLQNTGK 720

Query: 740 VKHEGYVFGTLVWYDGKHSVKSPIAINLG 769
           VK+   VFGTL+W DGKH V+S IA++LG
Sbjct: 721 VKNGSDVFGTLIWSDGKHFVRSSIAVHLG 746

BLAST of Sed0002069 vs. ExPASy TrEMBL
Match: A0A1S4DX90 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491001 PE=3 SV=1)

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 558/779 (71.63%), Postives = 639/779 (82.03%), Query Frame = 0

Query: 6   ISPLLLLLLLFFSL------LQTSTI--AIKKSYIV--------ALDPSSNLSTHFQQVT 65
           IS L + LLLF S       L  STI      SYIV        +LDPSS LS H QQVT
Sbjct: 74  ISNLKMALLLFESASGLNINLNKSTIFPINVPSYIVYLGSEHSSSLDPSS-LSEHSQQVT 133

Query: 66  AFHYDLLGSLLGSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARK 125
           A HYDLLGS LGSK MA+EAIFYSYTRSFNGFAA LD+KEA +LARNP VISV ENKARK
Sbjct: 134 ALHYDLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARK 193

Query: 126 LHTTRSWNFLGLEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPS 185
           LHTTRSWNFLG+E + G IPSNS+W AA+FG+D+I+ NID+GVWPESKSFSDEGYG VPS
Sbjct: 194 LHTTRSWNFLGVENDIG-IPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPS 253

Query: 186 KWRGICQTDSNFYCNKKLIGGRYFHKGYEAAGGVLDPSLLTVRDHDGHGTHTLSTAAGNF 245
           KWRGICQTDSNF+CN+KLIGGRYFHKGYEAAGG L+ +LLTVRDHDGHGTHTLSTAAGNF
Sbjct: 254 KWRGICQTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNF 313

Query: 246 VAGANVFGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISG 305
           V GANVFGHGNGTAKGGAPKAR VAYKVCWPP     CFDADI+AAFEAAIADGVDV+S 
Sbjct: 314 VTGANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLST 373

Query: 306 SIGATAGEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRD 365
           S+G  A E+FNDP+A+ +F AVQ+GI VV SGGN GP P TV+NV+PWV TVAA+T+DRD
Sbjct: 374 SLGGAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRD 433

Query: 366 FASYVVLGNKKILKGVSLSSVAPLPEKFYPLITAVDAKFSNITDLSAQSCGAGTLDPIKV 425
           F SYV LGNKK +KGVSLSSVA LP+KFYPLI +VDAKFSN+T+  AQ CG GTLDP+KV
Sbjct: 434 FVSYVGLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKV 493

Query: 426 KGKILICVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGDAEL 485
           KGKI+IC  G  + V+KGY A+ AGA G+I+AN  EKGDEI+P+LHF+PASDIT+ DA++
Sbjct: 494 KGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQI 553

Query: 486 LKQYLTSTKTPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDITAPGVTILAS 545
           +++YL STKTP AHL +VKT L VKPAP++A+FSSRGPN + S ILKPD+TAPGV ILAS
Sbjct: 554 VQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILAS 613

Query: 546 YPTVTPPFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIMTTAK 605
           YPT   P  S  D RRIPFN +SGTSMSCPH++GI  L+K IHP WSP+AIKSAIMTTAK
Sbjct: 614 YPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAK 673

Query: 606 TLDNNLKTILDWTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGYNAMDI 665
           T  NN +TILD TKL+ATP+AYG G V PN A DPGLVYD T  DYLNFLCARGY+AM I
Sbjct: 674 TRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKI 733

Query: 666 KKFYDKPFTCDKSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTYVAQVKVSPQ 725
           KKFY KPF C +SF V DLNYPS S+GE LK+GA +TINRR+KNVGSPGTYVA+VK SP 
Sbjct: 734 KKFYAKPFNCVRSFKVTDLNYPSISVGE-LKIGAPLTINRRVKNVGSPGTYVARVKASPG 793

Query: 726 VSVSVKPSMLQFSRLGEEKAFKVVVQNKGKVKHEGYVFGTLVWYDGKHSVKSPIAINLG 769
           V+V+++PS L F  +GEEK FKVV+QN GKVK  G VFGTL+W DGKH V+SPIA++LG
Sbjct: 794 VAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVK-SGDVFGTLIWSDGKHFVRSPIAVHLG 848

BLAST of Sed0002069 vs. ExPASy TrEMBL
Match: A0A5D3D763 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00150 PE=3 SV=1)

HSP 1 Score: 1067.4 bits (2759), Expect = 2.8e-308
Identity = 550/788 (69.80%), Postives = 631/788 (80.08%), Query Frame = 0

Query: 1   MEASNISPLLLLLLLFFSLLQTSTIAIKKSYIV--------ALDPSSNLSTHFQQVTAFH 60
           MEASN+SP LLL   +FSLLQTSTIA KKSYIV        +LDPSS LS H QQVTA H
Sbjct: 1   MEASNLSP-LLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSS-LSEHSQQVTALH 60

Query: 61  YDLLGSLLGSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHT 120
           YDLLGS LGSK MA+EAIFYSYTRSFNGFAA LD+KEA +LARNP VISV ENKARKLHT
Sbjct: 61  YDLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHT 120

Query: 121 TRSWNFLGLEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWR 180
           TRSWNFLG+E + G IPSNS+W AA+FG+D+I+ NID+GVWPESKSFSDEGYG VPSKWR
Sbjct: 121 TRSWNFLGVENDIG-IPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWR 180

Query: 181 GICQTDSNFYCNKKLIGGRYFHKGYEAAGGVLDPSLLTVRDHDGHGTHTLSTAAGNFVAG 240
           GICQTDSNF CN+KLIGGRYFHKGYEAAGG L+ +LLTVRDHDGHGTHTLSTAAGNFV G
Sbjct: 181 GICQTDSNFLCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTG 240

Query: 241 ANVFGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISGSIG 300
           ANVFGHGNGTAKGGAPKAR VAYKVCWPP     CFDADI+AAFEAAIADGVDV+S S+G
Sbjct: 241 ANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLG 300

Query: 301 ATAGEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRDFAS 360
             A E+FNDP+A+ +F AVQ+GI VV SGGN GP P TV+NV+PWV TVAA+T+DRDF S
Sbjct: 301 GAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVS 360

Query: 361 YVVLGNKKILK--------GVSLSSVAP----LPEKFYPLITAVDAKFSNITDLSAQSCG 420
           YV LGNKK +K        G+  S  AP     PE +            +  + S Q CG
Sbjct: 361 YVGLGNKKHIKVHVYTHLLGLGASRFAPNFICAPELYN------QYSIGSTLETSNQFCG 420

Query: 421 AGTLDPIKVKGKILICVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPAS 480
            GTLDP+KVKGKI+IC  G  + V+KGY A+ AGA G+I+AN  EKGDEI+P+LHF+PAS
Sbjct: 421 KGTLDPMKVKGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPAS 480

Query: 481 DITSGDAELLKQYLTSTKTPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDIT 540
           DIT+ DA+++++YL STKTP AHL +VKT L VKPAP++A+FSSRGPN + S ILKPD+T
Sbjct: 481 DITNTDAQIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVT 540

Query: 541 APGVTILASYPTVTPPFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAI 600
           APGV ILASYPT   P  S  D RRIPFN +SGTSMSCPH++GI  L+K IHP WSP+AI
Sbjct: 541 APGVNILASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAI 600

Query: 601 KSAIMTTAKTLDNNLKTILDWTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLC 660
           KSAIMTTAKT  NN +TILD TKL+ATP+AYG G V PN A DPGLVYD T  DYLNFLC
Sbjct: 601 KSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLC 660

Query: 661 ARGYNAMDIKKFYDKPFTCDKSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTY 720
           ARGY+AM IKKFY KPF C +SF V DLNYPS S+GE LK+GA +TINRR+KNVGSPGTY
Sbjct: 661 ARGYDAMKIKKFYAKPFNCVRSFKVTDLNYPSISVGE-LKIGAPLTINRRVKNVGSPGTY 720

Query: 721 VAQVKVSPQVSVSVKPSMLQFSRLGEEKAFKVVVQNKGKVKHEGYVFGTLVWYDGKHSVK 769
           VA+VK SP V+V+++PS L F  +GEEK FKVV+QN GKVK  G VFGTL+W DGKH V+
Sbjct: 721 VARVKASPGVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVK-SGDVFGTLIWSDGKHFVR 777

BLAST of Sed0002069 vs. ExPASy TrEMBL
Match: A0A6J1CQS4 (subtilisin-like protease SBT5.3 OS=Momordica charantia OX=3673 GN=LOC111013337 PE=3 SV=1)

HSP 1 Score: 1052.0 bits (2719), Expect = 1.2e-303
Identity = 530/774 (68.48%), Postives = 620/774 (80.10%), Query Frame = 0

Query: 1   MEASNISPLLLLLLLFFSLLQTSTIAI-KKSYIVALDPSSNLST----HFQQVTAFHYDL 60
           MEASNISPLLL   L  SLLQTSTIAI KKSYIV L   S+L++      +Q+TA HY+L
Sbjct: 1   MEASNISPLLLAFFL-LSLLQTSTIAIDKKSYIVYLGSQSSLASTSLLDSRQLTASHYNL 60

Query: 61  LGSLLGSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHTTRS 120
           LGSLLGSKK+A+E+IFYSYTRSFNGFAA LDE  AA LA+NP VISV EN+ARKLHTT S
Sbjct: 61  LGSLLGSKKIAEESIFYSYTRSFNGFAATLDENVAARLAKNPQVISVFENRARKLHTTHS 120

Query: 121 WNFLGLEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWRGIC 180
           WNFLG+E  QG  P NS+W AA+FGQD+IV NID+GVWPESKSFSDEG+G +PS+W+G C
Sbjct: 121 WNFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSRWKGTC 180

Query: 181 QTDSNFYCNKKLIGGRYFHKGYEAAGGVLDPSLLTVRDHDGHGTHTLSTAAGNFVAGANV 240
           QT  NF+CN+KLIGGRYF+KGYEAAG  L PS LTVRDHDGHGTHTL+TAAGNFV GANV
Sbjct: 181 QTYLNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANV 240

Query: 241 FGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISGSIGATA 300
           FG GNGTAKGGAP+ARV AYKVCWPP   G CFDAD +A FEAAIADGVDVIS S+G + 
Sbjct: 241 FGQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSV 300

Query: 301 GEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRDFASYVV 360
            +F +DP+A+ +FHA+QQGI  V S GN+GP PG+VSNVAPW  TVAAST+DRDFASY+ 
Sbjct: 301 RDFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIA 360

Query: 361 LGNKKILKGVSLSSVAPLPEKFYPLITAVDAKF--SNITDLSAQSCGAGTLDPIKVKGKI 420
           LGNKK +KG SL+SVAPLP+KFYPLI +V  +    N+TD  AQ CG GT DP KVKGKI
Sbjct: 361 LGNKKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKI 420

Query: 421 LICVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGDAELLKQY 480
           L+C+ G +   EKG  A + GA G+I+ N  + GD+IFP+LHFLPASD+   D ++L QY
Sbjct: 421 LVCLEGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTQY 480

Query: 481 LTSTKTPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDITAPGVTILASYPTV 540
           + ST+ P  HL  V+TELG+KPAPVMA FSSRGPN +  +ILKPDITAPGV ILA+Y + 
Sbjct: 481 MDSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSG 540

Query: 541 TPPFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIMTTAKTLDN 600
             P  S  D RRIPF+ +SGTSMSCPHISGI ALLK IHP+WSP+AIKSAIMTTAKT DN
Sbjct: 541 NAPTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDN 600

Query: 601 NLKTILDWTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGYNAMDIKKFY 660
           NL +ILD TK++ATPFAYG GHV PN AMDPGLVYD T +DYLNFLCARGY A  I++FY
Sbjct: 601 NLGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFY 660

Query: 661 DKPFTCDKSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTYVAQVKVSPQVSVS 720
            K + CD+SF + DLNYPS S+   L +G  VT+NRR+KNVGS GTYVA+VKV P+VS+S
Sbjct: 661 AKSYVCDRSFKLTDLNYPSISVSN-LTMG-PVTVNRRVKNVGSLGTYVARVKVHPEVSIS 720

Query: 721 VKPSMLQFSRLGEEKAFKVVVQNKGKVKHEGYVFGTLVWYDGKHSVKSPIAINL 768
           V+PS LQFS +GEEKAFKVV Q KGK K EG +FGTL+W DGKH V+SPI +NL
Sbjct: 721 VEPSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNL 771

BLAST of Sed0002069 vs. ExPASy TrEMBL
Match: A0A6J1L3L8 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498821 PE=3 SV=1)

HSP 1 Score: 1004.2 bits (2595), Expect = 2.9e-289
Identity = 514/774 (66.41%), Postives = 604/774 (78.04%), Query Frame = 0

Query: 1   MEASNISPLLLLLLLFFSLLQTS---TIAIKKSYIVALDPSS----NLSTHFQQVTAFHY 60
           MEASN+ P +L L   FSLLQTS   TIA KK YIV L+  S    +     QQV  FHY
Sbjct: 1   MEASNLIPSILFLFFLFSLLQTSTIATIATKKPYIVYLESQSVYADSKHCSIQQVQEFHY 60

Query: 61  DLLGSLLGSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHTT 120
           +LLGSLLGS + A+E+I YSYTRSFNGFAA L+EKEAADLAR+P VISVLEN+ RKLHTT
Sbjct: 61  NLLGSLLGSPEAAKESILYSYTRSFNGFAAILNEKEAADLARSPQVISVLENRGRKLHTT 120

Query: 121 RSWNFLGLEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWRG 180
            SW FLG+E ++G IPSNS+W AA+FG+DMI+ NID+GVWPES SFSDEGYG +PSKWRG
Sbjct: 121 NSWRFLGVEDDEG-IPSNSIWNAAQFGRDMIIANIDTGVWPESNSFSDEGYGPIPSKWRG 180

Query: 181 ICQTDSNFYCNKKLIGGRYFHKGYEAAGGVLDPSLLTVRDHDGHGTHTLSTAAGNFVAGA 240
            C  D +F CN+KLIGGRYF+KGY+ AGGVL+ + +++RDH+GHGTHTLSTAAGNFV GA
Sbjct: 181 TCPDDPSFRCNRKLIGGRYFYKGYKFAGGVLNATSVSIRDHNGHGTHTLSTAAGNFVPGA 240

Query: 241 NVFGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISGSIGA 300
           N+FGHGNGTAKGGAPKARV AYK CWP    G CFDAD++AAFEAAI DGVDVIS S+G 
Sbjct: 241 NIFGHGNGTAKGGAPKARVAAYKACWPLIPEGECFDADVLAAFEAAINDGVDVISASLGG 300

Query: 301 TAGEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRDFASY 360
              EFF DP+ + +FHA QQGI V+ S GN+GP P TV NVAPW  TVAAST  R+F S 
Sbjct: 301 GDQEFFRDPVGIAAFHAAQQGIIVIFSAGNDGPQPETVGNVAPWEITVAASTTGRNFVSN 360

Query: 361 VVLGNKKILKGVSLSSVAPLPEKFYPLITAVDAKFSNITDLSAQSCGAGTLDPIKVKGKI 420
           V LGN KILKG SLSSV+ LP+ FYPLI +V+AKFSN+++  A+ CG GTL+P KVKGKI
Sbjct: 361 VALGNNKILKGSSLSSVSALPQ-FYPLIDSVNAKFSNVSEFQAKFCGKGTLNPKKVKGKI 420

Query: 421 LICVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGDAELLKQY 480
           LIC AG +  VEKGY+AA+AGAVGMILA   +  +EI P+L+FLPAS IT  D ELL  Y
Sbjct: 421 LICTAGKITGVEKGYNAAEAGAVGMILATNIDNLEEIKPELYFLPASGITYSDGELLDDY 480

Query: 481 LTSTKTPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDITAPGVTILASYPTV 540
           + ST TP A + NV+TE+ + P+PV+A+FSSRGPN     ILKPDITAPG TILASYPT 
Sbjct: 481 INSTSTPVAQIMNVRTEVEINPSPVVAAFSSRGPNPFDKAILKPDITAPGFTILASYPTT 540

Query: 541 TPPFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIMTTAKTLDN 600
             P  S  D RR PFN  SGTSM+CPHIS I ALLK IHP+WSP+AIKSA+MTTAKT DN
Sbjct: 541 RAPTRSGFDQRRTPFNVFSGTSMACPHISAIAALLKSIHPEWSPAAIKSALMTTAKTSDN 600

Query: 601 NLKTILDWTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGYNAMDIKKFY 660
           N   +L    L ATPFA+G GHV PN AMDPGLVYD T ++YLNFLCARGYNA+ +++F 
Sbjct: 601 N--NLL----LNATPFAFGAGHVRPNDAMDPGLVYDITVDEYLNFLCARGYNAIQLRRFS 660

Query: 661 DKPFTCDKSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTYVAQVKVSPQVSVS 720
            +PF C+KSF   DLNYPS SI   L V A VTINRR+KNVG PGTYVA+V++   V+ S
Sbjct: 661 KQPFVCEKSFKAIDLNYPSISIPN-LNVNAPVTINRRVKNVGGPGTYVARVELPEGVAAS 720

Query: 721 VKPSMLQFSRLGEEKAFKVVVQNKGKVKHEGYVFGTLVWYDGKHSVKSPIAINL 768
           V+PS LQFS +GEEKAF+VVVQN G++KHEGYVFG LVW DGKHSV SPI++NL
Sbjct: 721 VEPSTLQFSSVGEEKAFRVVVQNTGELKHEGYVFGKLVWSDGKHSVGSPISMNL 765

BLAST of Sed0002069 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 796.6 bits (2056), Expect = 1.8e-230
Identity = 413/771 (53.57%), Postives = 531/771 (68.87%), Query Frame = 0

Query: 5   NISPLLLLLLLFFSLLQTSTIAIKKSYIVALDPSSNL----STHFQQVTAFHYDLLGSLL 64
           N S LLLLLL+  S           SY+V     S++         +V   HYD LGS  
Sbjct: 6   NFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFT 65

Query: 65  GSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHTTRSWNFLG 124
           GS++ A +AIFYSYT+  NGFAA LD   A +++++P V+SV  NKA KLHTTRSW+FLG
Sbjct: 66  GSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLG 125

Query: 125 LEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWRGICQT--D 184
           LE     +PS+S+W+ A FG+D I+ N+D+GVWPESKSF DEG G +PS+W+GICQ   D
Sbjct: 126 LE-HNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKD 185

Query: 185 SNFYCNKKLIGGRYFHKGYEAAGGVLDPSLLTVRDHDGHGTHTLSTAAGNFVAGANVFGH 244
           + F+CN+KLIG RYF+KGY AA G L+ S  + RD DGHG+HTLSTAAG+FV G ++FG 
Sbjct: 186 ATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQ 245

Query: 245 GNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISGSIGATAGEF 304
           GNGTAKGG+P+ARV AYKVCWPP  G  C+DAD++AAF+AAI DG DVIS S+G     F
Sbjct: 246 GNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSF 305

Query: 305 FNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRDFASYVVLGN 364
           FND +A+ SFHA ++ I VV S GN GP   TVSNVAPW  TV ASTMDR+FAS +VLGN
Sbjct: 306 FNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGN 365

Query: 365 KKILKGVSLSSVAPLPEKFYPLITAVDAKFSNITDLSAQSCGAGTLDPIKVKGKILICVA 424
            K  KG SLSS A    KFYP++ +V+AK  N + L AQ C  G+LDPIK KGKIL+C+ 
Sbjct: 366 GKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLR 425

Query: 425 GGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGDAELLKQYLTSTK 484
           G    VEKG   A  G +GM+L N    G+++    H LPA+ +TS D+  + +Y++ TK
Sbjct: 426 GQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTK 485

Query: 485 TPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDITAPGVTILASYPTVTPPFP 544
            P AH+   +T+LG+KPAPVMASFSS+GP++V   ILKPDITAPGV+++A+Y     P  
Sbjct: 486 KPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTN 545

Query: 545 STNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIMTTAKTLDNNLKTI 604
              D RR+ FNA+SGTSMSCPHISGI  LLK  +P WSP+AI+SAIMTTA  +D+    I
Sbjct: 546 EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPI 605

Query: 605 LDWTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGYNAMDIKKFYDKPFT 664
            + T ++ATPF++G GHV PN A++PGLVYD   +DYLNFLC+ GYNA  I  F    FT
Sbjct: 606 QNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFT 665

Query: 665 CDK-SFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTYVAQVKVSPQVSVSVKPS 724
           C      + +LNYPS ++       + VT++R +KNVG P  Y  +V     V V+VKP+
Sbjct: 666 CSSPKISLVNLNYPSITVPNL--TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPT 725

Query: 725 MLQFSRLGEEKAFKVV-VQNKGKVKHEGYVFGTLVWYDGKHSVKSPIAINL 768
            L F+++GE+K FKV+ V++KG V  +GYVFG LVW D KH V+SPI + L
Sbjct: 726 SLNFTKVGEQKTFKVILVKSKGNVA-KGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Sed0002069 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 796.6 bits (2056), Expect = 1.8e-230
Identity = 425/774 (54.91%), Postives = 527/774 (68.09%), Query Frame = 0

Query: 1   MEASNISPLLLLLLLFFSLLQTSTIAIKKSYIVALDPSSNL----STHFQQVTAFHYDLL 60
           M   ++S LLLL+ LFFS       A+KKSYIV L   ++L    S H   V   H   L
Sbjct: 16  MSLQSLSSLLLLVTLFFS----PAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFL 75

Query: 61  GSLLGSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHTTRSW 120
            S +GS + A+EAIFYSY R  NGFAA LDE EAA++A++P+V+SV  NK RKLHTT SW
Sbjct: 76  ASFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSW 135

Query: 121 NFLGLEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWRGICQ 180
           NF+ L K  G +  +SLW  A +G+D I+ N+D+GVWPESKSFSDEGYGAVP++W+G C 
Sbjct: 136 NFMLLAK-NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH 195

Query: 181 TDSNFYCNKKLIGGRYFHKGYEAAGGV-LDPSLLTVRDHDGHGTHTLSTAAGNFVAGANV 240
            D    CN+KLIG RYF+KGY A  G+  + S  T RDHDGHG+HTLSTAAGNFV GANV
Sbjct: 196 KD--VPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANV 255

Query: 241 FGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISGSIGATA 300
           FG GNGTA GG+PKARV AYKVCWPP +G  CFDADI+AA EAAI DGVDV+S S+G  A
Sbjct: 256 FGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDA 315

Query: 301 GEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRDFASYVV 360
           G++ +D IA+ SFHAV+ G+ VV S GN GP  GTVSNVAPWV TV AS+MDR+F ++V 
Sbjct: 316 GDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVE 375

Query: 361 LGNKKILKGVSLSSVAPLP-EKFYPLITAVDAKFSNITDLSAQSCGAGTLDPIKVKGKIL 420
           L N +  KG SLS   PLP EK Y LI+A DA  +N     A  C  G+LDP KVKGKIL
Sbjct: 376 LKNGQSFKGTSLSK--PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKIL 435

Query: 421 ICVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGDAELLKQYL 480
           +C+ G    V+KG  AA AGA GM+L N    G+EI    H LPAS I   D E L  YL
Sbjct: 436 VCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYL 495

Query: 481 TSTKTPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDITAPGVTILASYPTVT 540
           +STK P+ ++K     L  KPAP MASFSSRGPN +   ILKPDITAPGV I+A++   T
Sbjct: 496 SSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEAT 555

Query: 541 PPFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIMTTAKTLDNN 600
            P    +D+RR PFN  SGTSMSCPHISG+V LLK +HP WSP+AI+SAIMTT++T +N 
Sbjct: 556 GPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNR 615

Query: 601 LKTILDWTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGYNAMDIKKFYD 660
            K ++D +  +A PF+YG GHV PN A  PGLVYD T  DYL+FLCA GYN   ++ F +
Sbjct: 616 RKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAE 675

Query: 661 KP-FTCDKSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTYVAQVKVSPQVSVS 720
            P +TC +  ++ D NYPS ++     +  S+T+ R+LKNVG P TY A+ +    V VS
Sbjct: 676 DPQYTCRQGANLLDFNYPSITVP---NLTGSITVTRKLKNVGPPATYNARFREPLGVRVS 735

Query: 721 VKPSMLQFSRLGEEKAFKVVVQNKGKVKHEGYVFGTLVWYDGKHSVKSPIAINL 768
           V+P  L F++ GE K F++ ++    V   GYVFG L W D  H V+SPI + L
Sbjct: 736 VEPKQLTFNKTGEVKIFQMTLRPL-PVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Sed0002069 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 597.8 bits (1540), Expect = 1.2e-170
Identity = 340/777 (43.76%), Postives = 475/777 (61.13%), Query Frame = 0

Query: 1   MEASNISPLLLLLLLFFSLLQTSTIAIKKSYIVALDPSSNLSTHFQQVTAFHYDLLGSLL 60
           M +S +S     LLL       S+ +  +   +     S + + F   + ++   L S+ 
Sbjct: 1   MSSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSIS 60

Query: 61  GSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHTTRSWNFLG 120
            S +     + Y+Y  + +GF+ +L ++EA  L   P VISVL     +LHTTR+  FLG
Sbjct: 61  DSAE-----LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLG 120

Query: 121 LEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWRGICQTDSN 180
           L++   +     L+  A    D++VG +D+GVWPESKS+SDEG+G +PS W+G C+  +N
Sbjct: 121 LDEHTAD-----LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTN 180

Query: 181 F---YCNKKLIGGRYFHKGYEAAGGVLDPS--LLTVRDHDGHGTHTLSTAAGNFVAGANV 240
           F    CN+KLIG R+F +GYE+  G +D S    + RD DGHGTHT STAAG+ V GA++
Sbjct: 181 FTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASL 240

Query: 241 FGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISGSIGATA 300
            G+ +GTA+G AP+ARV  YKVCW     G CF +DI+AA + AIAD V+V+S S+G   
Sbjct: 241 LGYASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGM 300

Query: 301 GEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRDFASYVV 360
            +++ D +A+ +F A+++GI V  S GN GP   ++SNVAPW+TTV A T+DRDF +  +
Sbjct: 301 SDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAI 360

Query: 361 LGNKKILKGVSLSSVAPLPEKFYPLITAVDAKFSNITDLSAQSCGAGTLDPIKVKGKILI 420
           LGN K   GVSL     LP+K  P I A +A  SN T  +   C  GTL P KVKGKI++
Sbjct: 361 LGNGKNFTGVSLFKGEALPDKLLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKIVM 420

Query: 421 CVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGDAELLKQYLT 480
           C  G    V+KG     AG VGMILAN    G+E+    H LPA+ +     ++++ Y+T
Sbjct: 421 CDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVT 480

Query: 481 STKTPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDITAPGVTILASYPTVTP 540
           +   P A +  + T +GVKP+PV+A+FSSRGPN +  NILKPD+ APGV ILA++     
Sbjct: 481 TDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAG 540

Query: 541 PFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIMTTAKTLDNNL 600
           P    +D RR+ FN +SGTSMSCPH+SG+ ALLK +HP+WSP+AI+SA+MTTA     + 
Sbjct: 541 PTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDG 600

Query: 601 KTILD-WTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGYNAMDIKKFYD 660
           K +LD  T   +TPF +G GHV P  A +PGL+YD T EDYL FLCA  Y +  I+    
Sbjct: 601 KPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR 660

Query: 661 KPFTCD--KSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGSPGTYVAQVKVSPQ--- 720
           + +TCD  KS+ V DLNYPSF++     VGA     R + +VG  GTY   VKV+ +   
Sbjct: 661 RNYTCDPSKSYSVADLNYPSFAV-NVDGVGA-YKYTRTVTSVGGAGTY--SVKVTSETTG 720

Query: 721 VSVSVKPSMLQFSRLGEEKAFKVVVQNKGKVKHEGYVFGTLVWYDGKHSVKSPIAIN 767
           V +SV+P++L F    E+K++ V              FG++ W DGKH V SP+AI+
Sbjct: 721 VKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Sed0002069 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 553.1 bits (1424), Expect = 3.4e-157
Identity = 337/795 (42.39%), Postives = 467/795 (58.74%), Query Frame = 0

Query: 1   MEASNISPLLLLLLLFFSLLQTSTIAIKKSYIVALDPSSNLSTHFQQVTAFHYDLLGSLL 60
           +  S+I  +  LLL FFS   +S+  + +SYIV +  S   S  F     +H  LL SL 
Sbjct: 4   LSLSSIFFVFPLLLCFFSPSSSSSDGL-ESYIVHVQRSHKPSL-FSSHNNWHVSLLRSLP 63

Query: 61  GSKKMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHTTRSWNFLG 120
            S + A   + YSY+R+ +GF+A+L   + A L R+P+VISV+ ++AR++HTT +  FLG
Sbjct: 64  SSPQPA--TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLG 123

Query: 121 LEKEQGNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWRGICQTDSN 180
             +  G      LW  + +G+D+IVG +D+G+WPE  SFSD G G +PS W+G C+   +
Sbjct: 124 FSQNSG------LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPD 183

Query: 181 F---YCNKKLIGGRYFHKGY---------EAAGGVLDPSLLTVRDHDGHGTHTLSTAAGN 240
           F    CN+KLIG R F++GY          AA     P     RD +GHGTHT STAAG+
Sbjct: 184 FPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSP-----RDTEGHGTHTASTAAGS 243

Query: 241 FVAGANVFGHGNGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVIS 300
            VA A+++ +  GTA G A KAR+ AYK+CW     G C+D+DI+AA + A+ADGV VIS
Sbjct: 244 VVANASLYQYARGTATGMASKARIAAYKICWT----GGCYDSDILAAMDQAVADGVHVIS 303

Query: 301 GSIGA--TAGEFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTM 360
            S+GA  +A E+  D IA+ +F A + GI V  S GN GP P T +N+APW+ TV AST+
Sbjct: 304 LSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTV 363

Query: 361 DRDFASYVVLGNKKILKGVSLSSVAPLPEKFYPLITAVDAKFSNITDLSAQSCGAGTLDP 420
           DR+FA+  + G+ K+  G SL +   LP+    L+ +         D  ++ C  G L+ 
Sbjct: 364 DREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVYS--------GDCGSRLCYPGKLNS 423

Query: 421 IKVKGKILICVAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGD 480
             V+GKI++C  GG   VEKG     AG  GMILAN  E G+E+    H +PA+ + +  
Sbjct: 424 SLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKA 483

Query: 481 AELLKQYLTSTKTPEAHLKNVKTELGVK-PAPVMASFSSRGPNLVVSNILKPDITAPGVT 540
            + ++ Y+ ++ +P A +  + T +G   P+P +A+FSSRGPN +   ILKPD+ APGV 
Sbjct: 484 GDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVN 543

Query: 541 ILASYPTVTPPFPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIM 600
           ILA +  +  P     D RR+ FN +SGTSMSCPH+SG+ ALL+  HP WSP+AIKSA++
Sbjct: 544 ILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALV 603

Query: 601 TTAKTLDNNLKTILDW-TKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGY 660
           TTA  ++N+ + I D  T   +  F +G GHVDPN A++PGLVYD   ++Y+ FLCA GY
Sbjct: 604 TTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGY 663

Query: 661 NAMDIKKFYDKPFTCD-----KSFHVRDLNYPSFSIGEALKVGASVTINRRLKNVGS--P 720
               I  F   P   D     K     DLNYPSFS+  A   G  V   R +KNVGS   
Sbjct: 664 EFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFA-STGEVVKYKRVVKNVGSNVD 723

Query: 721 GTYVAQVKVSPQVSVSVKPSMLQFSR----LGEEKAFKVVVQNKGKVKHEGYVFGTLVWY 769
             Y   VK    V + V PS L FS+    L  E  FK VV   G     G+ FG++ W 
Sbjct: 724 AVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWT 770

BLAST of Sed0002069 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 552.7 bits (1423), Expect = 4.5e-157
Identity = 329/779 (42.23%), Postives = 469/779 (60.21%), Query Frame = 0

Query: 7   SPLLLLLLLFFSLLQTSTIAIKKSYIVALDPSSNLSTHFQQVTAFHYDLLGSLLGSKKMA 66
           S ++LLL L F  +  +     K++I  +D  S  S      T +H+        S + A
Sbjct: 4   STIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIF---PTHYHW-------YSTEFA 63

Query: 67  QEA-IFYSYTRSFNGFAAKLDEKEAADLARNPNVISVLENKARKLHTTRSWNFLGLEKEQ 126
           +E+ I + Y   F+GF+A +   EA +L  +P V++V E++ R+LHTTRS  FLGL+ ++
Sbjct: 64  EESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQK 123

Query: 127 GNIPSNSLWKAAEFGQDMIVGNIDSGVWPESKSFSDEGYGAVPSKWRGICQTDSNF---Y 186
           G      LW  +++G D+I+G  D+G+WPE +SFSD   G +P +WRG+C++ + F    
Sbjct: 124 G------LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRN 183

Query: 187 CNKKLIGGRYFHKGYEAA--GGV-LDPSLLTVRDHDGHGTHTLSTAAGNFVAGANVFGHG 246
           CN+K+IG R+F KG +AA  GG+      L+ RD DGHGTHT STAAG     A++ G+ 
Sbjct: 184 CNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYA 243

Query: 247 NGTAKGGAPKARVVAYKVCWPPFNGGMCFDADIIAAFEAAIADGVDVISGSIGATAG--- 306
           +G AKG APKAR+ AYKVCW       C D+DI+AAF+AA+ DGVDVIS SIG   G   
Sbjct: 244 SGVAKGVAPKARIAAYKVCW---KDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITS 303

Query: 307 EFFNDPIAMISFHAVQQGIDVVLSGGNEGPLPGTVSNVAPWVTTVAASTMDRDFASYVVL 366
            ++ DPIA+ S+ A  +GI V  S GNEGP   +V+N+APWVTTV AST+DR+F +  +L
Sbjct: 304 PYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAIL 363

Query: 367 GNKKILKGVSLSSVAPLPEKFYPLITAVDAKFSNITDLSAQSCGAGTLDPIKVKGKILIC 426
           G+   L+GVSL +  PL  + +P++    +  S     SA  C   TLDP +V+GKI+IC
Sbjct: 364 GDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMS-----SASLCMENTLDPKQVRGKIVIC 423

Query: 427 VAGGLQPVEKGYHAAQAGAVGMILANLNEKGDEIFPQLHFLPASDITSGDAELLKQYLTS 486
             G    V KG    +AG VGMILAN    G+ +    H +PA  + S + + +K Y +S
Sbjct: 424 DRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASS 483

Query: 487 TKTPEAHLKNVKTELGVKPAPVMASFSSRGPNLVVSNILKPDITAPGVTILASYPTVTPP 546
              P A +    T +G+KPAPV+ASFS RGPN +   ILKPD+ APGV ILA++     P
Sbjct: 484 HPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGP 543

Query: 547 FPSTNDHRRIPFNALSGTSMSCPHISGIVALLKFIHPKWSPSAIKSAIMTTAKTLDNNLK 606
               +D R+  FN LSGTSM+CPH+SG  ALLK  HP WSP+ I+SA+MTT   +DN+ +
Sbjct: 544 TGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNR 603

Query: 607 TILD-WTKLEATPFAYGGGHVDPNAAMDPGLVYDATFEDYLNFLCARGYNAMDIKKFYDK 666
           +++D  T   ATP+ YG GH++   AM+PGLVYD T +DY+ FLC+ GY    I+     
Sbjct: 604 SLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRT 663

Query: 667 PFTC--DKSFHVRDLNYPSF-SIGEALKVG-ASVTINRRLKNVG-SPGTYVAQVKVSPQV 726
           P  C   +     +LNYPS  ++    + G  S T+ R   NVG +   Y A+++    V
Sbjct: 664 PVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGV 723

Query: 727 SVSVKPSMLQFSRLGEEKAFKVVVQNKGK---VKHEGYVFGTLVWYD-GKHSVKSPIAI 766
           +V+VKP  L F+   + +++ V V    +   +   G VFG++ W+D GKH V+SPI +
Sbjct: 724 TVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758