Sed0001993 (gene) Chayote v1

Overview
NameSed0001993
Typegene
OrganismSechium edule (Chayote v1)
DescriptionCysteine proteinase 1
LocationLG01: 3896190 .. 3897321 (+)
RNA-Seq ExpressionSed0001993
SyntenySed0001993
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTATGATTCTTCTCTGGGTTTTCTGGACACCTCCATCAACAGCATCCGTGTCTATGGAATACGATCAAGGATCTGGTTCCGTTGACCTACAAGACAGGTACCAGAAATGGATGAGTAAATACAGTCGAGAATACAAGAGCAGAGAGGAGCACGAGCGAAGATTCGCAATTTATCAGTTGAATGTTCAGTACATTGACCACTTCAATTCTCTGAATCGTTCACATACTCTGGCTGAAAATAATTTTGCAGACCTCACAAATGATGAGTTTAAGAAAATTTACCTGGGGTATCAAACTCAATGTCTTCCTGATACATGCTTCAGATATGGAAATATGGTTGATTTTCCTACTAGTGTTGACTGGAGAAAGCATGGTGCAGTTACTCCAATAAAGAATCAAGGCGAATGCGGTATGAACTCGTTCCTCTTAACTGATCAGAAATCACTATGAACCTGTTTTCTTTTTATATGATTTTGAGTTCTTTTGCTAAAAAGTAGCCTTGACCCCACATACTCACCAGTTTTGAACTATTGACACAGGGAGTTGCTGGGCATTCTCTGCAGTAGCAGCTGTGGAAGGCATAAACAAAATAAAAACAAGGAAATTAGTGTCTCTATCAGAACAAGAGCTAGTGGACTGCGATGTCAACTCGGATAACCAGGGATGCAGTGGCGGTTTAATGTACAAAGCATTTCAGTTCATCGAGAAAACTGGACTCACTACAGAAAGAGAATATCCATACAGAGGAACTGAAGATGTATGCAGCAAACAAAAAGAGAGACAACACTCTGTGAAAATAAGTGGGTATGAAAAAGTACCCGCCAATGATGAGAAAAGCTTACAAGCTGCAGTCGTTAAACAGCCAGTCTCTGTAGCAATTGATGCAGAGGGATCTGATTTCCAGTTCTATTCTGGTGGAGTCTTCTCAGGGATTTGTGGAAAGCAACTCAATCATGGAGTGGCAATAGTTGGGTATGGGGAAGCTAGAAATAAACCTTACTGGATCGTCAAGAATTCATGGGGCACGGACTGGGGTGAATCTGGTTACATAAGAATGGAACGTGATACCATTGATAAGCGAGGTACTTGTGGCATAGCAATGGAGGCTAGCTACCCTGTCAAAGACTGA

mRNA sequence

ATGTCTATGATTCTTCTCTGGGTTTTCTGGACACCTCCATCAACAGCATCCGTGTCTATGGAATACGATCAAGGATCTGGTTCCGTTGACCTACAAGACAGGTACCAGAAATGGATGAGTAAATACAGTCGAGAATACAAGAGCAGAGAGGAGCACGAGCGAAGATTCGCAATTTATCAGTTGAATGTTCAGTACATTGACCACTTCAATTCTCTGAATCGTTCACATACTCTGGCTGAAAATAATTTTGCAGACCTCACAAATGATGAGTTTAAGAAAATTTACCTGGGGTATCAAACTCAATGTCTTCCTGATACATGCTTCAGATATGGAAATATGGTTGATTTTCCTACTAGTGTTGACTGGAGAAAGCATGGTGCAGTTACTCCAATAAAGAATCAAGGCGAATGCGGGAGTTGCTGGGCATTCTCTGCAGTAGCAGCTGTGGAAGGCATAAACAAAATAAAAACAAGGAAATTAGTGTCTCTATCAGAACAAGAGCTAGTGGACTGCGATGTCAACTCGGATAACCAGGGATGCAGTGGCGGTTTAATGTACAAAGCATTTCAGTTCATCGAGAAAACTGGACTCACTACAGAAAGAGAATATCCATACAGAGGAACTGAAGATGTATGCAGCAAACAAAAAGAGAGACAACACTCTGTGAAAATAAGTGGGTATGAAAAAGTACCCGCCAATGATGAGAAAAGCTTACAAGCTGCAGTCGTTAAACAGCCAGTCTCTGTAGCAATTGATGCAGAGGGATCTGATTTCCAGTTCTATTCTGGTGGAGTCTTCTCAGGGATTTGTGGAAAGCAACTCAATCATGGAGTGGCAATAGTTGGGTATGGGGAAGCTAGAAATAAACCTTACTGGATCGTCAAGAATTCATGGGGCACGGACTGGGGTGAATCTGGTTACATAAGAATGGAACGTGATACCATTGATAAGCGAGGTACTTGTGGCATAGCAATGGAGGCTAGCTACCCTGTCAAAGACTGA

Coding sequence (CDS)

ATGTCTATGATTCTTCTCTGGGTTTTCTGGACACCTCCATCAACAGCATCCGTGTCTATGGAATACGATCAAGGATCTGGTTCCGTTGACCTACAAGACAGGTACCAGAAATGGATGAGTAAATACAGTCGAGAATACAAGAGCAGAGAGGAGCACGAGCGAAGATTCGCAATTTATCAGTTGAATGTTCAGTACATTGACCACTTCAATTCTCTGAATCGTTCACATACTCTGGCTGAAAATAATTTTGCAGACCTCACAAATGATGAGTTTAAGAAAATTTACCTGGGGTATCAAACTCAATGTCTTCCTGATACATGCTTCAGATATGGAAATATGGTTGATTTTCCTACTAGTGTTGACTGGAGAAAGCATGGTGCAGTTACTCCAATAAAGAATCAAGGCGAATGCGGGAGTTGCTGGGCATTCTCTGCAGTAGCAGCTGTGGAAGGCATAAACAAAATAAAAACAAGGAAATTAGTGTCTCTATCAGAACAAGAGCTAGTGGACTGCGATGTCAACTCGGATAACCAGGGATGCAGTGGCGGTTTAATGTACAAAGCATTTCAGTTCATCGAGAAAACTGGACTCACTACAGAAAGAGAATATCCATACAGAGGAACTGAAGATGTATGCAGCAAACAAAAAGAGAGACAACACTCTGTGAAAATAAGTGGGTATGAAAAAGTACCCGCCAATGATGAGAAAAGCTTACAAGCTGCAGTCGTTAAACAGCCAGTCTCTGTAGCAATTGATGCAGAGGGATCTGATTTCCAGTTCTATTCTGGTGGAGTCTTCTCAGGGATTTGTGGAAAGCAACTCAATCATGGAGTGGCAATAGTTGGGTATGGGGAAGCTAGAAATAAACCTTACTGGATCGTCAAGAATTCATGGGGCACGGACTGGGGTGAATCTGGTTACATAAGAATGGAACGTGATACCATTGATAAGCGAGGTACTTGTGGCATAGCAATGGAGGCTAGCTACCCTGTCAAAGACTGA

Protein sequence

MSMILLWVFWTPPSTASVSMEYDQGSGSVDLQDRYQKWMSKYSREYKSREEHERRFAIYQLNVQYIDHFNSLNRSHTLAENNFADLTNDEFKKIYLGYQTQCLPDTCFRYGNMVDFPTSVDWRKHGAVTPIKNQGECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSGGLMYKAFQFIEKTGLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAVVKQPVSVAIDAEGSDFQFYSGGVFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGTDWGESGYIRMERDTIDKRGTCGIAMEASYPVKD
Homology
BLAST of Sed0001993 vs. NCBI nr
Match: XP_038902939.1 (ervatamin-B [Benincasa hispida])

HSP 1 Score: 552.7 bits (1423), Expect = 2.1e-153
Identity = 264/330 (80.00%), Postives = 291/330 (88.18%), Query Frame = 0

Query: 4   ILLWVFWTPPSTASVSMEYDQGSGSVDLQDRYQKWMSKYSREYKSREEHERRFAIYQLNV 63
           ++LWV WT P+   ++M+Y  GS S DLQ RYQKWMSKY R+YKSREE ERRF IYQLNV
Sbjct: 15  LILWVIWT-PTMVFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNV 74

Query: 64  QYIDHFNSLNRSHTLAENNFADLTNDEFKKIYLGYQTQCLPDTCFRYGNMVDFPTSVDWR 123
           QYID+FNSL+ S+TLAENNFADLTNDEFK+ YLGY+T  LPDTCFRYGNMVD PT+V+WR
Sbjct: 75  QYIDNFNSLDHSYTLAENNFADLTNDEFKETYLGYKTDWLPDTCFRYGNMVDLPTNVNWR 134

Query: 124 KHGAVTPIKNQGECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSGG 183
           K GAVTPIKNQG+CGSCWAFSAVAAVEGINKIKT KL+SLSEQELVDCDV S NQGC+GG
Sbjct: 135 KEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGG 194

Query: 184 LMYKAFQFIEKTGLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAVV 243
            M KAFQFI+KTGLTTE EYPYRG E  C+KQK R H+V+ISGYEKVPANDEKSL+AAV 
Sbjct: 195 FMDKAFQFIKKTGLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAAVA 254

Query: 244 KQPVSVAIDAEGSDFQFYSGGVFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGTDWG 303
            QPVSVAIDA G DFQFYSGGVFSG CGKQLNHGVAIVGYG+A NK YW+VKNSWGTDWG
Sbjct: 255 NQPVSVAIDAGGYDFQFYSGGVFSGNCGKQLNHGVAIVGYGQASNKSYWLVKNSWGTDWG 314

Query: 304 ESGYIRMERDTIDKRGTCGIAMEASYPVKD 334
           ESGYIRM+RD+ DKRGTCGIAM ASYP+KD
Sbjct: 315 ESGYIRMKRDSTDKRGTCGIAMMASYPIKD 343

BLAST of Sed0001993 vs. NCBI nr
Match: KGN62053.1 (hypothetical protein Csa_006706 [Cucumis sativus])

HSP 1 Score: 535.4 bits (1378), Expect = 3.5e-148
Identity = 251/331 (75.83%), Postives = 286/331 (86.40%), Query Frame = 0

Query: 4   ILLWVFWTPPSTASVSMEYDQGSG-SVDLQDRYQKWMSKYSREYKSREEHERRFAIYQLN 63
           ++LWVFWT P   S++M+Y  GS  S D+QDRYQKWM KY R+YKSREE ERRF IYQ N
Sbjct: 11  LILWVFWT-PRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWERRFTIYQAN 70

Query: 64  VQYIDHFNSLNRSHTLAENNFADLTNDEFKKIYLGYQTQCLPDTCFRYGNMVDFPTSVDW 123
           VQYID+FNS+N SHTLAENNFADLTN+EFK  YLGY+T  +PDTCFRYGNMV+ PT+VDW
Sbjct: 71  VQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNMVNLPTNVDW 130

Query: 124 RKHGAVTPIKNQGECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSG 183
           R+ GAVTPIKNQG+CGSCWAFSAVAAVEGINKIK  KL+SLSEQELVDCDV S NQGC+G
Sbjct: 131 RQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNG 190

Query: 184 GLMYKAFQFIEKTGLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAV 243
           G MYKAF+FI++TGLTTE EYPY+G E  C++QKE+   V ISGYEKVP NDEKSL+AAV
Sbjct: 191 GYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAV 250

Query: 244 VKQPVSVAIDAEGSDFQFYSGGVFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGTDW 303
             QPVSVAIDAEG++FQFYSGG+FSG CG QLNHGVAIVGYGE  N+ YW+VKNSWGTDW
Sbjct: 251 ANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDW 310

Query: 304 GESGYIRMERDTIDKRGTCGIAMEASYPVKD 334
           GESGYIRM+RD+ D++GTCGIAM ASYP KD
Sbjct: 311 GESGYIRMKRDSTDRQGTCGIAMMASYPTKD 340

BLAST of Sed0001993 vs. NCBI nr
Match: XP_004148072.2 (ervatamin-B [Cucumis sativus])

HSP 1 Score: 535.4 bits (1378), Expect = 3.5e-148
Identity = 251/331 (75.83%), Postives = 286/331 (86.40%), Query Frame = 0

Query: 4   ILLWVFWTPPSTASVSMEYDQGSG-SVDLQDRYQKWMSKYSREYKSREEHERRFAIYQLN 63
           ++LWVFWT P   S++M+Y  GS  S D+QDRYQKWM KY R+YKSREE ERRF IYQ N
Sbjct: 27  LILWVFWT-PRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWERRFTIYQAN 86

Query: 64  VQYIDHFNSLNRSHTLAENNFADLTNDEFKKIYLGYQTQCLPDTCFRYGNMVDFPTSVDW 123
           VQYID+FNS+N SHTLAENNFADLTN+EFK  YLGY+T  +PDTCFRYGNMV+ PT+VDW
Sbjct: 87  VQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNMVNLPTNVDW 146

Query: 124 RKHGAVTPIKNQGECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSG 183
           R+ GAVTPIKNQG+CGSCWAFSAVAAVEGINKIK  KL+SLSEQELVDCDV S NQGC+G
Sbjct: 147 RQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNG 206

Query: 184 GLMYKAFQFIEKTGLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAV 243
           G MYKAF+FI++TGLTTE EYPY+G E  C++QKE+   V ISGYEKVP NDEKSL+AAV
Sbjct: 207 GYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAV 266

Query: 244 VKQPVSVAIDAEGSDFQFYSGGVFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGTDW 303
             QPVSVAIDAEG++FQFYSGG+FSG CG QLNHGVAIVGYGE  N+ YW+VKNSWGTDW
Sbjct: 267 ANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDW 326

Query: 304 GESGYIRMERDTIDKRGTCGIAMEASYPVKD 334
           GESGYIRM+RD+ D++GTCGIAM ASYP KD
Sbjct: 327 GESGYIRMKRDSTDRQGTCGIAMMASYPTKD 356

BLAST of Sed0001993 vs. NCBI nr
Match: AKO60151.1 (cysteine proteinase 1, partial [Citrullus lanatus])

HSP 1 Score: 531.9 bits (1369), Expect = 3.8e-147
Identity = 256/318 (80.50%), Postives = 280/318 (88.05%), Query Frame = 0

Query: 16  ASVSMEYDQGSGSVDLQDRYQKWMSKYSREYKSREEHERRFAIYQLNVQYIDHFNSLNRS 75
           AS+ M+Y  GS S DLQDRYQKWMSKY REYKSREE E+RF IYQLNVQYID+FNSLN S
Sbjct: 2   ASMEMDYRPGSSSGDLQDRYQKWMSKYGREYKSREEWEQRFNIYQLNVQYIDNFNSLNHS 61

Query: 76  HTLAENNFADLTNDEFKKIYLGYQTQCLPDTCFRYGNMVDFPTSVDWRKHGAVTPIKNQG 135
           +TLAEN+FADLTNDEFK  YLG++T  LPDT FRYGNMV+ PT+VDWRK  AVTP+K+QG
Sbjct: 62  YTLAENSFADLTNDEFKTTYLGFKTDWLPDTWFRYGNMVNLPTNVDWRKENAVTPVKDQG 121

Query: 136 ECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSGGLMYKAFQFIEKT 195
           +CGSCWAFSAVAAVEGINKIKT KL+SLSEQELVDCDV S NQGC+GG MYKAF+FI+KT
Sbjct: 122 QCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGYMYKAFEFIKKT 181

Query: 196 GLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAVVKQPVSVAIDAEG 255
           GLTTE EYPYRG E VC+KQK R  +V ISGYEKVP NDEKSL+AAV  QPVSVAIDA G
Sbjct: 182 GLTTEIEYPYRGIESVCNKQKVRYRTVTISGYEKVPVNDEKSLKAAVANQPVSVAIDAGG 241

Query: 256 SDFQFYSGGVFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGTDWGESGYIRMERDTI 315
            DFQFYSGGVFSG CGKQLNHGVAIVGYGEA NK YW+VKNSWGTDWGESGYIRM+RD+ 
Sbjct: 242 YDFQFYSGGVFSGNCGKQLNHGVAIVGYGEASNKTYWLVKNSWGTDWGESGYIRMKRDST 301

Query: 316 DKRGTCGIAMEASYPVKD 334
           DKRGTCGIAM ASYP+KD
Sbjct: 302 DKRGTCGIAMMASYPIKD 319

BLAST of Sed0001993 vs. NCBI nr
Match: XP_008458487.1 (PREDICTED: ervatamin-B-like [Cucumis melo] >KAA0033464.1 ervatamin-B-like [Cucumis melo var. makuwa])

HSP 1 Score: 525.8 bits (1353), Expect = 2.7e-145
Identity = 254/333 (76.28%), Postives = 282/333 (84.68%), Query Frame = 0

Query: 4   ILLWVFWTPPSTASVSMEYDQG-SGSVDLQDRYQKWMSKYSREYKSREEHERRFAIYQLN 63
           ++LWVFWT P+  S++M+Y  G S S +LQDRYQKWM KY R+YKSREE E+RF IYQ N
Sbjct: 15  LILWVFWT-PTRVSMAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSREEWEQRFTIYQAN 74

Query: 64  VQYIDHFNSLNRSHTLAENNFADLTNDEFKKIYLGYQTQCLP--DTCFRYGNMVDFPTSV 123
           VQYID+FNSLN S+TLAENNF DLTN+EF   YLGY+T  LP  DT FRYGNMV+ PT+V
Sbjct: 75  VQYIDNFNSLNHSYTLAENNFTDLTNEEFMATYLGYETVSLPDTDTFFRYGNMVNLPTNV 134

Query: 124 DWRKHGAVTPIKNQGECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGC 183
           DWRK GAVTPIKNQG+CGSCWAFSAVAAVEGINKIK  KL+SLSEQELVDCDV S NQGC
Sbjct: 135 DWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVTSGNQGC 194

Query: 184 SGGLMYKAFQFIEKTGLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQA 243
           +GG MYKAF+FI+KTGLTTE EYPY  T   C KQKE+  SV ISGYEKVP NDEKSLQA
Sbjct: 195 NGGYMYKAFEFIKKTGLTTELEYPYTATRSACDKQKEKYQSVSISGYEKVPVNDEKSLQA 254

Query: 244 AVVKQPVSVAIDAEGSDFQFYSGGVFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGT 303
           AV KQPVSVAIDA G+DFQFYSGG+FSG CGKQLNHGVAIVGYGE  N+ YW+VKNSWGT
Sbjct: 255 AVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDSNQAYWLVKNSWGT 314

Query: 304 DWGESGYIRMERDTIDKRGTCGIAMEASYPVKD 334
            WGESGYIRM RD+ DK+GTCGIAM ASYP+KD
Sbjct: 315 SWGESGYIRMMRDSTDKQGTCGIAMMASYPIKD 346

BLAST of Sed0001993 vs. ExPASy Swiss-Prot
Match: Q9FGR9 (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 PE=1 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.9e-93
Identity = 174/313 (55.59%), Postives = 218/313 (69.65%), Query Frame = 0

Query: 31  LQDRYQKWMSKYSREYKSREEHERRFAIYQLNVQYIDHFNSLNRSHTLAENNFADLTNDE 90
           L + Y++W S ++   +S EE  +RF +++ NV++I   N  ++S+ L  N F D+T++E
Sbjct: 34  LWELYERWRSHHT-VARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEE 93

Query: 91  FKKIYLG--------YQTQCLPDTCFRYGNMVDFPTSVDWRKHGAVTPIKNQGECGSCWA 150
           F++ Y G        +Q +      F Y N+   PTSVDWRK+GAVTP+KNQG+CGSCWA
Sbjct: 94  FRRTYAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWA 153

Query: 151 FSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSGGLMYKAFQFI-EKTGLTTER 210
           FS V AVEGIN+I+T+KL SLSEQELVDCD N  NQGC+GGLM  AF+FI EK GLT+E 
Sbjct: 154 FSTVVAVEGINQIRTKKLTSLSEQELVDCDTN-QNQGCNGGLMDLAFEFIKEKGGLTSEL 213

Query: 211 EYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAVVKQPVSVAIDAEGSDFQFY 270
            YPY+ +++ C   KE    V I G+E VP N E  L  AV  QPVSVAIDA GSDFQFY
Sbjct: 214 VYPYKASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFY 273

Query: 271 SGGVFSGICGKQLNHGVAIVGYGEA-RNKPYWIVKNSWGTDWGESGYIRMERDTIDKRGT 330
           S GVF+G CG +LNHGVA+VGYG       YWIVKNSWG +WGE GYIRM+R    K G 
Sbjct: 274 SEGVFTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGL 333

Query: 331 CGIAMEASYPVKD 334
           CGIAMEASYP+K+
Sbjct: 334 CGIAMEASYPLKN 344

BLAST of Sed0001993 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.1e-92
Identity = 179/343 (52.19%), Postives = 223/343 (65.01%), Query Frame = 0

Query: 3   MILLWVFWTPPSTASVSMEYD--QGSGSVDLQDRYQKWMSKYSREYKSREEHERRFAIYQ 62
           ++L+++F       +   +YD  +      L   Y +W S +S   +S  E E+RF +++
Sbjct: 4   LLLIFLFSLVILQTACGFDYDDKEIESEEGLSTLYDRWRSHHSVP-RSLNEREKRFNVFR 63

Query: 63  LNVQYIDHFNSLNRSHTLAENNFADLTNDEFKKIYLGYQ----------TQCLPDTCFRY 122
            NV ++ + N  NRS+ L  N FADLT +EFK  Y G             +      + +
Sbjct: 64  HNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFMYDH 123

Query: 123 GNMVDFPTSVDWRKHGAVTPIKNQGECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVD 182
            N+   P+SVDWRK GAVT IKNQG+CGSCWAFS VAAVEGINKIKT KLVSLSEQELVD
Sbjct: 124 ENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQELVD 183

Query: 183 CDVNSDNQGCSGGLMYKAFQFIEKT-GLTTEREYPYRGTEDVCSKQKERQHSVKISGYEK 242
           CD    N+GC+GGLM  AF+FI+K  G+TTE  YPY G +  C   K+    V I G+E 
Sbjct: 184 CD-TKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGHED 243

Query: 243 VPANDEKSLQAAVVKQPVSVAIDAEGSDFQFYSGGVFSGICGKQLNHGVAIVGYGEARNK 302
           VP NDE +L  AV  QPVSVAIDA  SDFQFYS GVF+G CG +LNHGVA VGYG  R K
Sbjct: 244 VPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYGSERGK 303

Query: 303 PYWIVKNSWGTDWGESGYIRMERDTIDKRGTCGIAMEASYPVK 333
            YWIV+NSWG +WGE GYI++ER+  +  G CGIAMEASYP+K
Sbjct: 304 KYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of Sed0001993 vs. ExPASy Swiss-Prot
Match: Q9FJ47 (Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana OX=3702 GN=SAG12 PE=1 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.0e-90
Identity = 169/316 (53.48%), Postives = 216/316 (68.35%), Query Frame = 0

Query: 31  LQDRYQKWMSKYSREYKSREEHERRFAIYQLNVQYIDHFNSL--NRSHTLAENNFADLTN 90
           +Q R+ +WM+K+ R Y   +E   R+ +++ NV+ I+H NS+   R+  LA N FADLTN
Sbjct: 34  MQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTN 93

Query: 91  DEFKKIYLGY----------QTQCLPDTCFRYGNMVD--FPTSVDWRKHGAVTPIKNQGE 150
           DEF+ +Y G+          QT+  P   FRY N+     P SVDWRK GAVTPIKNQG 
Sbjct: 94  DEFRSMYTGFKGVSALSSQSQTKMSP---FRYQNVSSGALPVSVDWRKKGAVTPIKNQGS 153

Query: 151 CGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSGGLMYKAFQFIEKT- 210
           CG CWAFSAVAA+EG  +IK  KL+SLSEQ+LVDCD N  + GC GGLM  AF+ I+ T 
Sbjct: 154 CGCCWAFSAVAAIEGATQIKKGKLISLSEQQLVDCDTN--DFGCEGGLMDTAFEHIKATG 213

Query: 211 GLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAVVKQPVSVAIDAEG 270
           GLTTE  YPY+G +  C+ +K    +  I+GYE VP NDE++L  AV  QPVSV I+  G
Sbjct: 214 GLTTESNYPYKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGG 273

Query: 271 SDFQFYSGGVFSGICGKQLNHGVAIVGYGEARN-KPYWIVKNSWGTDWGESGYIRMERDT 330
            DFQFYS GVF+G C   L+H V  +GYGE+ N   YWI+KNSWGT WGESGY+R+++D 
Sbjct: 274 FDFQFYSSGVFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKDV 333

BLAST of Sed0001993 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 9.9e-90
Identity = 173/343 (50.44%), Postives = 220/343 (64.14%), Query Frame = 0

Query: 5   LLWVFWTPPSTASVSMEYDQGSGSVD----LQDRYQKWMSKYSREYKSREEHERRFAIYQ 64
           LLWV  +      V+  +D     ++    L D Y++W S ++      E+H +RF +++
Sbjct: 6   LLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKH-KRFNVFK 65

Query: 65  LNVQYIDHFNSLNRSHTLAENNFADLTNDEFKKIYLG--------YQTQCLPDTCFRYGN 124
            NV ++ + N +++ + L  N FAD+TN EF+  Y G        ++        F Y  
Sbjct: 66  ANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGSGTFMYEK 125

Query: 125 MVDFPTSVDWRKHGAVTPIKNQGECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCD 184
           +   P SVDWRK GAVT +K+QG+CGSCWAFS + AVEGIN+IKT KLVSLSEQELVDCD
Sbjct: 126 VGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELVDCD 185

Query: 185 VNSDNQGCSGGLMYKAFQFI-EKTGLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVP 244
              +NQGC+GGLM  AF+FI +K G+TTE  YPY   E  C + K    +V I G+E VP
Sbjct: 186 -KEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDGHENVP 245

Query: 245 ANDEKSLQAAVVKQPVSVAIDAEGSDFQFYSGGVFSGICGKQLNHGVAIVGYG-EARNKP 304
            NDE +L  AV  QPVSVAIDA GSDFQFYS GVF+G C   LNHGVAIVGYG       
Sbjct: 246 VNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCNTDLNHGVAIVGYGTTVDGTN 305

Query: 305 YWIVKNSWGTDWGESGYIRMERDTIDKRGTCGIAMEASYPVKD 334
           YWIV+NSWG +WGE GYIRM+R+   K G CGIAM ASYP+K+
Sbjct: 306 YWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIKN 346

BLAST of Sed0001993 vs. ExPASy Swiss-Prot
Match: O65493 (Cysteine protease XCP1 OS=Arabidopsis thaliana OX=3702 GN=XCP1 PE=1 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 9.9e-90
Identity = 170/308 (55.19%), Postives = 211/308 (68.51%), Query Frame = 0

Query: 31  LQDRYQKWMSKYSREYKSREEHERRFAIYQLNVQYIDHFNSLNRSHTLAENNFADLTNDE 90
           L + ++ WMS++S+ YKS EE   RF +++ N+ +ID  N+   S+ L  N FADLT++E
Sbjct: 47  LLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEE 106

Query: 91  FKKIYLG-----YQTQCLPDTCFRYGNMVDFPTSVDWRKHGAVTPIKNQGECGSCWAFSA 150
           FK  YLG     +  +  P   FRY ++ D P SVDWRK GAV P+K+QG+CGSCWAFS 
Sbjct: 107 FKGRYLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSCWAFST 166

Query: 151 VAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSGGLMYKAFQFIEKT-GLTTEREYP 210
           VAAVEGIN+I T  L SLSEQEL+DCD  + N GC+GGLM  AFQ+I  T GL  E +YP
Sbjct: 167 VAAVEGINQITTGNLSSLSEQELIDCD-TTFNSGCNGGLMDYAFQYIISTGGLHKEDDYP 226

Query: 211 YRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAVVKQPVSVAIDAEGSDFQFYSGG 270
           Y   E +C +QKE    V ISGYE VP ND++SL  A+  QPVSVAI+A G DFQFY GG
Sbjct: 227 YLMEEGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVAIEASGRDFQFYKGG 286

Query: 271 VFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGTDWGESGYIRMERDTIDKRGTCGIA 330
           VF+G CG  L+HGVA VGYG ++   Y IVKNSWG  WGE G+IRM+R+T    G CGI 
Sbjct: 287 VFNGKCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGKPEGLCGIN 346

Query: 331 MEASYPVK 333
             ASYP K
Sbjct: 347 KMASYPTK 353

BLAST of Sed0001993 vs. ExPASy TrEMBL
Match: A0A0A0LJV6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292830 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.7e-148
Identity = 251/331 (75.83%), Postives = 286/331 (86.40%), Query Frame = 0

Query: 4   ILLWVFWTPPSTASVSMEYDQGSG-SVDLQDRYQKWMSKYSREYKSREEHERRFAIYQLN 63
           ++LWVFWT P   S++M+Y  GS  S D+QDRYQKWM KY R+YKSREE ERRF IYQ N
Sbjct: 11  LILWVFWT-PRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWERRFTIYQAN 70

Query: 64  VQYIDHFNSLNRSHTLAENNFADLTNDEFKKIYLGYQTQCLPDTCFRYGNMVDFPTSVDW 123
           VQYID+FNS+N SHTLAENNFADLTN+EFK  YLGY+T  +PDTCFRYGNMV+ PT+VDW
Sbjct: 71  VQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNMVNLPTNVDW 130

Query: 124 RKHGAVTPIKNQGECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSG 183
           R+ GAVTPIKNQG+CGSCWAFSAVAAVEGINKIK  KL+SLSEQELVDCDV S NQGC+G
Sbjct: 131 RQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNG 190

Query: 184 GLMYKAFQFIEKTGLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAV 243
           G MYKAF+FI++TGLTTE EYPY+G E  C++QKE+   V ISGYEKVP NDEKSL+AAV
Sbjct: 191 GYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAV 250

Query: 244 VKQPVSVAIDAEGSDFQFYSGGVFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGTDW 303
             QPVSVAIDAEG++FQFYSGG+FSG CG QLNHGVAIVGYGE  N+ YW+VKNSWGTDW
Sbjct: 251 ANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDW 310

Query: 304 GESGYIRMERDTIDKRGTCGIAMEASYPVKD 334
           GESGYIRM+RD+ D++GTCGIAM ASYP KD
Sbjct: 311 GESGYIRMKRDSTDRQGTCGIAMMASYPTKD 340

BLAST of Sed0001993 vs. ExPASy TrEMBL
Match: A0A384S0D9 (Cysteine proteinase 1 (Fragment) OS=Citrullus lanatus OX=3654 GN=ClCP1 PE=2 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 1.9e-147
Identity = 256/318 (80.50%), Postives = 280/318 (88.05%), Query Frame = 0

Query: 16  ASVSMEYDQGSGSVDLQDRYQKWMSKYSREYKSREEHERRFAIYQLNVQYIDHFNSLNRS 75
           AS+ M+Y  GS S DLQDRYQKWMSKY REYKSREE E+RF IYQLNVQYID+FNSLN S
Sbjct: 2   ASMEMDYRPGSSSGDLQDRYQKWMSKYGREYKSREEWEQRFNIYQLNVQYIDNFNSLNHS 61

Query: 76  HTLAENNFADLTNDEFKKIYLGYQTQCLPDTCFRYGNMVDFPTSVDWRKHGAVTPIKNQG 135
           +TLAEN+FADLTNDEFK  YLG++T  LPDT FRYGNMV+ PT+VDWRK  AVTP+K+QG
Sbjct: 62  YTLAENSFADLTNDEFKTTYLGFKTDWLPDTWFRYGNMVNLPTNVDWRKENAVTPVKDQG 121

Query: 136 ECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSGGLMYKAFQFIEKT 195
           +CGSCWAFSAVAAVEGINKIKT KL+SLSEQELVDCDV S NQGC+GG MYKAF+FI+KT
Sbjct: 122 QCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGYMYKAFEFIKKT 181

Query: 196 GLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAVVKQPVSVAIDAEG 255
           GLTTE EYPYRG E VC+KQK R  +V ISGYEKVP NDEKSL+AAV  QPVSVAIDA G
Sbjct: 182 GLTTEIEYPYRGIESVCNKQKVRYRTVTISGYEKVPVNDEKSLKAAVANQPVSVAIDAGG 241

Query: 256 SDFQFYSGGVFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGTDWGESGYIRMERDTI 315
            DFQFYSGGVFSG CGKQLNHGVAIVGYGEA NK YW+VKNSWGTDWGESGYIRM+RD+ 
Sbjct: 242 YDFQFYSGGVFSGNCGKQLNHGVAIVGYGEASNKTYWLVKNSWGTDWGESGYIRMKRDST 301

Query: 316 DKRGTCGIAMEASYPVKD 334
           DKRGTCGIAM ASYP+KD
Sbjct: 302 DKRGTCGIAMMASYPIKD 319

BLAST of Sed0001993 vs. ExPASy TrEMBL
Match: A0A5A7SQK0 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001250 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 254/333 (76.28%), Postives = 282/333 (84.68%), Query Frame = 0

Query: 4   ILLWVFWTPPSTASVSMEYDQG-SGSVDLQDRYQKWMSKYSREYKSREEHERRFAIYQLN 63
           ++LWVFWT P+  S++M+Y  G S S +LQDRYQKWM KY R+YKSREE E+RF IYQ N
Sbjct: 15  LILWVFWT-PTRVSMAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSREEWEQRFTIYQAN 74

Query: 64  VQYIDHFNSLNRSHTLAENNFADLTNDEFKKIYLGYQTQCLP--DTCFRYGNMVDFPTSV 123
           VQYID+FNSLN S+TLAENNF DLTN+EF   YLGY+T  LP  DT FRYGNMV+ PT+V
Sbjct: 75  VQYIDNFNSLNHSYTLAENNFTDLTNEEFMATYLGYETVSLPDTDTFFRYGNMVNLPTNV 134

Query: 124 DWRKHGAVTPIKNQGECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGC 183
           DWRK GAVTPIKNQG+CGSCWAFSAVAAVEGINKIK  KL+SLSEQELVDCDV S NQGC
Sbjct: 135 DWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVTSGNQGC 194

Query: 184 SGGLMYKAFQFIEKTGLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQA 243
           +GG MYKAF+FI+KTGLTTE EYPY  T   C KQKE+  SV ISGYEKVP NDEKSLQA
Sbjct: 195 NGGYMYKAFEFIKKTGLTTELEYPYTATRSACDKQKEKYQSVSISGYEKVPVNDEKSLQA 254

Query: 244 AVVKQPVSVAIDAEGSDFQFYSGGVFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGT 303
           AV KQPVSVAIDA G+DFQFYSGG+FSG CGKQLNHGVAIVGYGE  N+ YW+VKNSWGT
Sbjct: 255 AVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDSNQAYWLVKNSWGT 314

Query: 304 DWGESGYIRMERDTIDKRGTCGIAMEASYPVKD 334
            WGESGYIRM RD+ DK+GTCGIAM ASYP+KD
Sbjct: 315 SWGESGYIRMMRDSTDKQGTCGIAMMASYPIKD 346

BLAST of Sed0001993 vs. ExPASy TrEMBL
Match: A0A1S3C828 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103497881 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 254/333 (76.28%), Postives = 282/333 (84.68%), Query Frame = 0

Query: 4   ILLWVFWTPPSTASVSMEYDQG-SGSVDLQDRYQKWMSKYSREYKSREEHERRFAIYQLN 63
           ++LWVFWT P+  S++M+Y  G S S +LQDRYQKWM KY R+YKSREE E+RF IYQ N
Sbjct: 15  LILWVFWT-PTRVSMAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSREEWEQRFTIYQAN 74

Query: 64  VQYIDHFNSLNRSHTLAENNFADLTNDEFKKIYLGYQTQCLP--DTCFRYGNMVDFPTSV 123
           VQYID+FNSLN S+TLAENNF DLTN+EF   YLGY+T  LP  DT FRYGNMV+ PT+V
Sbjct: 75  VQYIDNFNSLNHSYTLAENNFTDLTNEEFMATYLGYETVSLPDTDTFFRYGNMVNLPTNV 134

Query: 124 DWRKHGAVTPIKNQGECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGC 183
           DWRK GAVTPIKNQG+CGSCWAFSAVAAVEGINKIK  KL+SLSEQELVDCDV S NQGC
Sbjct: 135 DWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVTSGNQGC 194

Query: 184 SGGLMYKAFQFIEKTGLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQA 243
           +GG MYKAF+FI+KTGLTTE EYPY  T   C KQKE+  SV ISGYEKVP NDEKSLQA
Sbjct: 195 NGGYMYKAFEFIKKTGLTTELEYPYTATRSACDKQKEKYQSVSISGYEKVPVNDEKSLQA 254

Query: 244 AVVKQPVSVAIDAEGSDFQFYSGGVFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGT 303
           AV KQPVSVAIDA G+DFQFYSGG+FSG CGKQLNHGVAIVGYGE  N+ YW+VKNSWGT
Sbjct: 255 AVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDSNQAYWLVKNSWGT 314

Query: 304 DWGESGYIRMERDTIDKRGTCGIAMEASYPVKD 334
            WGESGYIRM RD+ DK+GTCGIAM ASYP+KD
Sbjct: 315 SWGESGYIRMMRDSTDKQGTCGIAMMASYPIKD 346

BLAST of Sed0001993 vs. ExPASy TrEMBL
Match: A0A6J1CH04 (ervatamin-B OS=Momordica charantia OX=3673 GN=LOC111011342 PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.2e-141
Identity = 246/330 (74.55%), Postives = 279/330 (84.55%), Query Frame = 0

Query: 4   ILLWVFWTPPSTASVSMEYDQGSGSVDLQDRYQKWMSKYSREYKSREEHERRFAIYQLNV 63
           ++L VFWT  S ASV+ +   G GS D++DRYQKW+ KY REYKS EE E+RF IYQ NV
Sbjct: 15  LILCVFWT-LSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGEEREKRFPIYQSNV 74

Query: 64  QYIDHFNSLNRSHTLAENNFADLTNDEFKKIYLGYQTQCLPDTCFRYGNMVDFPTSVDWR 123
           QYID+FNSLNRS+TLA+N FADLTNDEFK  YLGY T   PDTCF+YGN+V+ PT+VDWR
Sbjct: 75  QYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKYGNIVNLPTNVDWR 134

Query: 124 KHGAVTPIKNQGECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSGG 183
           K GAVTPIK+QG+CGSCWAFSAVAAVEGI KIKT KLVSLSEQEL+DCDV S NQGCSGG
Sbjct: 135 KEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLDCDVISGNQGCSGG 194

Query: 184 LMYKAFQFIEKTGLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAVV 243
            M KAF+FI+K G+TTE+EYPYRG E+VC+KQK R HS  ISGYEKVPANDEKSL+AAV 
Sbjct: 195 FMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKVPANDEKSLKAAVA 254

Query: 244 KQPVSVAIDAEGSDFQFYSGGVFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGTDWG 303
            QPVSVAIDA G DFQFYSGG+FSG CGKQLNHGV IVGYGE   K YW+VKNSWGT WG
Sbjct: 255 NQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKSYWLVKNSWGTSWG 314

Query: 304 ESGYIRMERDTIDKRGTCGIAMEASYPVKD 334
           E GY+RM+ ++ DKRGTCGIAM+ASYP+KD
Sbjct: 315 EYGYVRMKSNSSDKRGTCGIAMDASYPIKD 343

BLAST of Sed0001993 vs. TAIR 10
Match: AT1G06260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 345.5 bits (885), Expect = 4.7e-95
Identity = 175/309 (56.63%), Postives = 212/309 (68.61%), Query Frame = 0

Query: 31  LQDRYQKWMSKYSREYKSREEHERRFAIYQLNVQYIDHFNSLNRSHTLAENNFADLTNDE 90
           L+ R++KW+  +S+ Y  R+E   RF IYQ NVQ ID+ NSL+    L +N FAD+TN E
Sbjct: 39  LKQRFEKWLKTHSKLYGGRDEWMLRFGIYQSNVQLIDYINSLHLPFKLTDNRFADMTNSE 98

Query: 91  FKKIYLGYQTQCL------PDTCFRYGNMVDFPTSVDWRKHGAVTPIKNQGECGSCWAFS 150
           FK  +LG  T  L         C   GN+   P +VDWR  GAVTPI+NQG+CG CWAFS
Sbjct: 99  FKAHFLGLNTSSLRLHKKQRPVCDPAGNV---PDAVDWRTQGAVTPIRNQGKCGGCWAFS 158

Query: 151 AVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSGGLMYKAFQFIEKT-GLTTEREY 210
           AVAA+EGINKIKT  LVSLSEQ+L+DCDV + N+GCSGGLM  AF+FI+   GL TE +Y
Sbjct: 159 AVAAIEGINKIKTGNLVSLSEQQLIDCDVGTYNKGCSGGLMETAFEFIKTNGGLATETDY 218

Query: 211 PYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAVVKQPVSVAIDAEGSDFQFYSG 270
           PY G E  C ++K +   V I GY+KV A +E SLQ A  +QPVSV IDA G  FQ YS 
Sbjct: 219 PYTGIEGTCDQEKSKNKVVTIQGYQKV-AQNEASLQIAAAQQPVSVGIDAGGFIFQLYSS 278

Query: 271 GVFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGTDWGESGYIRMERDTIDKRGTCGI 330
           GVF+  CG  LNHGV +VGYG   ++ YWIVKNSWGT WGE GYIRMER   +  G CGI
Sbjct: 279 GVFTNYCGTNLNHGVTVVGYGVEGDQKYWIVKNSWGTGWGEEGYIRMERGVSEDTGKCGI 338

Query: 331 AMEASYPVK 333
           AM ASYP++
Sbjct: 339 AMMASYPLQ 343

BLAST of Sed0001993 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 344.0 bits (881), Expect = 1.4e-94
Identity = 174/313 (55.59%), Postives = 218/313 (69.65%), Query Frame = 0

Query: 31  LQDRYQKWMSKYSREYKSREEHERRFAIYQLNVQYIDHFNSLNRSHTLAENNFADLTNDE 90
           L + Y++W S ++   +S EE  +RF +++ NV++I   N  ++S+ L  N F D+T++E
Sbjct: 34  LWELYERWRSHHT-VARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEE 93

Query: 91  FKKIYLG--------YQTQCLPDTCFRYGNMVDFPTSVDWRKHGAVTPIKNQGECGSCWA 150
           F++ Y G        +Q +      F Y N+   PTSVDWRK+GAVTP+KNQG+CGSCWA
Sbjct: 94  FRRTYAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWA 153

Query: 151 FSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSGGLMYKAFQFI-EKTGLTTER 210
           FS V AVEGIN+I+T+KL SLSEQELVDCD N  NQGC+GGLM  AF+FI EK GLT+E 
Sbjct: 154 FSTVVAVEGINQIRTKKLTSLSEQELVDCDTN-QNQGCNGGLMDLAFEFIKEKGGLTSEL 213

Query: 211 EYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAVVKQPVSVAIDAEGSDFQFY 270
            YPY+ +++ C   KE    V I G+E VP N E  L  AV  QPVSVAIDA GSDFQFY
Sbjct: 214 VYPYKASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFY 273

Query: 271 SGGVFSGICGKQLNHGVAIVGYGEA-RNKPYWIVKNSWGTDWGESGYIRMERDTIDKRGT 330
           S GVF+G CG +LNHGVA+VGYG       YWIVKNSWG +WGE GYIRM+R    K G 
Sbjct: 274 SEGVFTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGL 333

Query: 331 CGIAMEASYPVKD 334
           CGIAMEASYP+K+
Sbjct: 334 CGIAMEASYPLKN 344

BLAST of Sed0001993 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 340.5 bits (872), Expect = 1.5e-93
Identity = 179/343 (52.19%), Postives = 223/343 (65.01%), Query Frame = 0

Query: 3   MILLWVFWTPPSTASVSMEYD--QGSGSVDLQDRYQKWMSKYSREYKSREEHERRFAIYQ 62
           ++L+++F       +   +YD  +      L   Y +W S +S   +S  E E+RF +++
Sbjct: 4   LLLIFLFSLVILQTACGFDYDDKEIESEEGLSTLYDRWRSHHSVP-RSLNEREKRFNVFR 63

Query: 63  LNVQYIDHFNSLNRSHTLAENNFADLTNDEFKKIYLGYQ----------TQCLPDTCFRY 122
            NV ++ + N  NRS+ L  N FADLT +EFK  Y G             +      + +
Sbjct: 64  HNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFMYDH 123

Query: 123 GNMVDFPTSVDWRKHGAVTPIKNQGECGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVD 182
            N+   P+SVDWRK GAVT IKNQG+CGSCWAFS VAAVEGINKIKT KLVSLSEQELVD
Sbjct: 124 ENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQELVD 183

Query: 183 CDVNSDNQGCSGGLMYKAFQFIEKT-GLTTEREYPYRGTEDVCSKQKERQHSVKISGYEK 242
           CD    N+GC+GGLM  AF+FI+K  G+TTE  YPY G +  C   K+    V I G+E 
Sbjct: 184 CD-TKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGHED 243

Query: 243 VPANDEKSLQAAVVKQPVSVAIDAEGSDFQFYSGGVFSGICGKQLNHGVAIVGYGEARNK 302
           VP NDE +L  AV  QPVSVAIDA  SDFQFYS GVF+G CG +LNHGVA VGYG  R K
Sbjct: 244 VPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYGSERGK 303

Query: 303 PYWIVKNSWGTDWGESGYIRMERDTIDKRGTCGIAMEASYPVK 333
            YWIV+NSWG +WGE GYI++ER+  +  G CGIAMEASYP+K
Sbjct: 304 KYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of Sed0001993 vs. TAIR 10
Match: AT5G45890.1 (senescence-associated gene 12 )

HSP 1 Score: 334.0 bits (855), Expect = 1.4e-91
Identity = 169/316 (53.48%), Postives = 216/316 (68.35%), Query Frame = 0

Query: 31  LQDRYQKWMSKYSREYKSREEHERRFAIYQLNVQYIDHFNSL--NRSHTLAENNFADLTN 90
           +Q R+ +WM+K+ R Y   +E   R+ +++ NV+ I+H NS+   R+  LA N FADLTN
Sbjct: 34  MQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTN 93

Query: 91  DEFKKIYLGY----------QTQCLPDTCFRYGNMVD--FPTSVDWRKHGAVTPIKNQGE 150
           DEF+ +Y G+          QT+  P   FRY N+     P SVDWRK GAVTPIKNQG 
Sbjct: 94  DEFRSMYTGFKGVSALSSQSQTKMSP---FRYQNVSSGALPVSVDWRKKGAVTPIKNQGS 153

Query: 151 CGSCWAFSAVAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSGGLMYKAFQFIEKT- 210
           CG CWAFSAVAA+EG  +IK  KL+SLSEQ+LVDCD N  + GC GGLM  AF+ I+ T 
Sbjct: 154 CGCCWAFSAVAAIEGATQIKKGKLISLSEQQLVDCDTN--DFGCEGGLMDTAFEHIKATG 213

Query: 211 GLTTEREYPYRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAVVKQPVSVAIDAEG 270
           GLTTE  YPY+G +  C+ +K    +  I+GYE VP NDE++L  AV  QPVSV I+  G
Sbjct: 214 GLTTESNYPYKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGG 273

Query: 271 SDFQFYSGGVFSGICGKQLNHGVAIVGYGEARN-KPYWIVKNSWGTDWGESGYIRMERDT 330
            DFQFYS GVF+G C   L+H V  +GYGE+ N   YWI+KNSWGT WGESGY+R+++D 
Sbjct: 274 FDFQFYSSGVFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKDV 333

BLAST of Sed0001993 vs. TAIR 10
Match: AT4G35350.1 (xylem cysteine peptidase 1 )

HSP 1 Score: 331.6 bits (849), Expect = 7.1e-91
Identity = 170/308 (55.19%), Postives = 211/308 (68.51%), Query Frame = 0

Query: 31  LQDRYQKWMSKYSREYKSREEHERRFAIYQLNVQYIDHFNSLNRSHTLAENNFADLTNDE 90
           L + ++ WMS++S+ YKS EE   RF +++ N+ +ID  N+   S+ L  N FADLT++E
Sbjct: 47  LLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEE 106

Query: 91  FKKIYLG-----YQTQCLPDTCFRYGNMVDFPTSVDWRKHGAVTPIKNQGECGSCWAFSA 150
           FK  YLG     +  +  P   FRY ++ D P SVDWRK GAV P+K+QG+CGSCWAFS 
Sbjct: 107 FKGRYLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSCWAFST 166

Query: 151 VAAVEGINKIKTRKLVSLSEQELVDCDVNSDNQGCSGGLMYKAFQFIEKT-GLTTEREYP 210
           VAAVEGIN+I T  L SLSEQEL+DCD  + N GC+GGLM  AFQ+I  T GL  E +YP
Sbjct: 167 VAAVEGINQITTGNLSSLSEQELIDCD-TTFNSGCNGGLMDYAFQYIISTGGLHKEDDYP 226

Query: 211 YRGTEDVCSKQKERQHSVKISGYEKVPANDEKSLQAAVVKQPVSVAIDAEGSDFQFYSGG 270
           Y   E +C +QKE    V ISGYE VP ND++SL  A+  QPVSVAI+A G DFQFY GG
Sbjct: 227 YLMEEGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVAIEASGRDFQFYKGG 286

Query: 271 VFSGICGKQLNHGVAIVGYGEARNKPYWIVKNSWGTDWGESGYIRMERDTIDKRGTCGIA 330
           VF+G CG  L+HGVA VGYG ++   Y IVKNSWG  WGE G+IRM+R+T    G CGI 
Sbjct: 287 VFNGKCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGKPEGLCGIN 346

Query: 331 MEASYPVK 333
             ASYP K
Sbjct: 347 KMASYPTK 353

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902939.12.1e-15380.00ervatamin-B [Benincasa hispida][more]
KGN62053.13.5e-14875.83hypothetical protein Csa_006706 [Cucumis sativus][more]
XP_004148072.23.5e-14875.83ervatamin-B [Cucumis sativus][more]
AKO60151.13.8e-14780.50cysteine proteinase 1, partial [Citrullus lanatus][more]
XP_008458487.12.7e-14576.28PREDICTED: ervatamin-B-like [Cucumis melo] >KAA0033464.1 ervatamin-B-like [Cucum... [more]
Match NameE-valueIdentityDescription
Q9FGR91.9e-9355.59KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 ... [more]
Q9STL42.1e-9252.19KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ... [more]
Q9FJ472.0e-9053.48Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana OX=3702 GN=S... [more]
P124129.9e-9050.44Vignain OS=Vigna mungo OX=3915 PE=1 SV=1[more]
O654939.9e-9055.19Cysteine protease XCP1 OS=Arabidopsis thaliana OX=3702 GN=XCP1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LJV61.7e-14875.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292830 PE=3 SV=1[more]
A0A384S0D91.9e-14780.50Cysteine proteinase 1 (Fragment) OS=Citrullus lanatus OX=3654 GN=ClCP1 PE=2 SV=1[more]
A0A5A7SQK01.3e-14576.28Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001... [more]
A0A1S3C8281.3e-14576.28ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103497881 PE=3 SV=1[more]
A0A6J1CH041.2e-14174.55ervatamin-B OS=Momordica charantia OX=3673 GN=LOC111011342 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G06260.14.7e-9556.63Cysteine proteinases superfamily protein [more]
AT5G50260.11.4e-9455.59Cysteine proteinases superfamily protein [more]
AT3G48340.11.5e-9352.19Cysteine proteinases superfamily protein [more]
AT5G45890.11.4e-9153.48senescence-associated gene 12 [more]
AT4G35350.17.1e-9155.19xylem cysteine peptidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 134..149
score: 65.58
coord: 276..286
score: 54.87
coord: 291..297
score: 79.08
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 116..331
e-value: 1.1E-120
score: 416.9
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 117..331
e-value: 4.2E-85
score: 285.3
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 35..91
e-value: 2.4E-19
score: 80.3
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 35..91
e-value: 8.2E-13
score: 48.6
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 16..333
e-value: 6.9E-118
score: 395.8
NoneNo IPR availablePANTHERPTHR12411:SF796ERVATAMIN-B-LIKEcoord: 22..332
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 22..332
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 291..310
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 274..284
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 134..145
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 117..330
e-value: 1.14436E-112
score: 323.807
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 31..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0001993.1Sed0001993.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008234 cysteine-type peptidase activity