Sed0001988 (gene) Chayote v1

BLAST of Sed0001988 vs. NCBI nr
Match: XP_022953917.1 (putative disease resistance protein RGA4 [Cucurbita moschata])

HSP 1 Score: 947.6 bits (2448), Expect = 8.7e-272
Identity = 532/972 (54.73%), Postives = 663/972 (68.21%), Query Frame = 0

Query: 1   MAEGVLSNFAGNVLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSK 60
           MAEGVL N A +++ +LS+ A +QLGML G+KD+L  LKS+VSAI+AV+ DAEER TKS 
Sbjct: 1   MAEGVLFNLAADIIKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEERQTKSH 60

Query: 61  LLKDWLNKLGEALYDADDVLDEISTQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRMAL 120
            LKDWL KL EALYDA+DVLDEIS +A+R     RG  AKQVRIFFS+ N +AF+Y+M  
Sbjct: 61  FLKDWLEKLKEALYDAEDVLDEISGEAVR-----RGRKAKQVRIFFSRYNPIAFDYKMTR 120

Query: 121 KLKNIRGRLDEISEEKNKFGLIEKSEARTR---YDGLSHQIGTHSAFIEGEVVGREDDKM 180
           ++KN+R RL  I+ E+  F L E    +T+   +D +     THS     EV+GR+D K 
Sbjct: 121 QIKNVRERLGVIAAERKNFHLTEIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHKR 180

Query: 181 RIKSLLLDETDHNNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEFDVK 240
            IK LL D    +N+SFIAIVGM GIGKTTLA+ L+ND+Q+S+ FDLK+WVWVS  F+VK
Sbjct: 181 VIKDLLQDMNVKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFEVK 240

Query: 241 RVVEKMIESASGSKPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPLLMG 300
            VVEK+IESA+ S+PNV  M+ L  +LQK+++GKKYLLVMDDIWNESE+KW +LK LLMG
Sbjct: 241 IVVEKIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLLMG 300

Query: 301 CACGSKILITKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIEDANPKMIQL 360
            A GSKI+ITKRDS +  E+ +MT LI LKGLS++ SWSLF+KVAF   IE  NPK+IQL
Sbjct: 301 GARGSKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLIQL 360

Query: 361 GKEILKKCGGVPLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNSMTIIFELSYKHL 420
           GKEIL KCGGVPLVIRHIGRLLY   SE EWISFKN++L  V Q+ + M  I +LSY HL
Sbjct: 361 GKEILVKCGGVPLVIRHIGRLLYIEKSEEEWISFKNNQLLEVIQRDHDMASILKLSYNHL 420

Query: 421 PPHLKQCFAYSSLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCWRFF 480
           P +LKQCFAYSSL  K   I KD+L++QWVA GFVQS  G KSMED  NDYF ELCWRFF
Sbjct: 421 PSNLKQCFAYSSLLLKYRLIHKDELIRQWVAQGFVQSLNGCKSMEDTGNDYFMELCWRFF 480

Query: 481 Y-----EHQDGNNVCMHDMMRDLARMVASTGYARGNLKDED--GERIHHISLGHDIESWN 540
           Y     EH   ++V +HD+M DLARMVA   Y  GN   +   GE   HI    +I  W 
Sbjct: 481 YENFNDEHNFEDDVYIHDVMHDLARMVAGNEYVGGNQNHDHVVGESTRHILFDREIGIWE 540

Query: 541 DLPPELQKAKGLRTFHKS-----RMNHINEGVLDKLFSTFPHLRVLNMSWTEFKTVPDSL 600
            +P +L+KAKGLRTF  S     R N +NE VLD+LF  F  LRVL++ ++  K VPDS+
Sbjct: 541 HVPSKLRKAKGLRTFILSNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRVPDSI 600

Query: 601 NKLRHLRYLNLSDNRYLISLPNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRHLDLD 660
            +L HLRYL+LS N Y+ SLPNS++ LVNLQTL L  C  L +LPR  +NLVNLRHL++ 
Sbjct: 601 KRLTHLRYLDLSGN-YMKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRHLNIS 660

Query: 661 SVSKLTHTPKGIRKLTCLQTLNLFVLD--ENKQGSKLGELNELRDLRGGLYIKGLEQVRG 720
             + LTH P+G+ KLTCLQTL+ FVLD   ++ GSKL ELN L DLRG L I GLE ++ 
Sbjct: 661 DSTHLTHMPEGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLENLKF 720

Query: 721 NPSEARFANLKGKKGLRDLVLQWKPSSNEDESEDGLNDEIILEGLEPHPNVESVCIWRYK 780
            PSEA  ANLK KKGLR L L WK  S ++       DE IL GLEP+P +ES  I  Y 
Sbjct: 721 TPSEASLANLKEKKGLRHLTLIWKNLSGDEHRFQA--DEEILAGLEPNPAIESFRISGYF 780

Query: 781 GEGLPNW--MTNLVSKLTHISLAKCNRLQRLPQLDQLPTLKKLHLQELTCLQFIDKNNPG 840
           G  LPNW  +++ + KLT I +  C RLQ LPQ+  LP LK L+L  LT L+FID  +P 
Sbjct: 781 GVKLPNWLSLSSPLMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKFIDNTDPY 840

Query: 841 SSKSVFFPCLDILKLKNMPSLEGWWGGVLVGDGGSTKTRRLLPKFARLTRLYIYDCPKLG 900
           SS ++FFP L+ L L +MP+LE WW      +  S      LP F  LT L I +CPKL 
Sbjct: 841 SS-ALFFPSLESLCLMDMPNLEAWW------ERESMVEEEFLPTFPCLTILSISNCPKLA 900

Query: 901 WVPRQPSLEKVELNNVGVRMVRSI------GPSLYLEYVTLKNIEDLVSLPKKLCS--LT 946
            +P  P L  + L+ VG++M+ SI       PS  L+ + LK IEDL +L  KL     +
Sbjct: 901 TMPGHPPLTDITLDGVGLQMMSSIRSSSNTSPSSSLKSLELKGIEDLKNLSHKLRQNLNS 957

BLAST of Sed0001988 vs. NCBI nr
Match: XP_023547878.1 (putative disease resistance protein RGA3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 945.3 bits (2442), Expect = 4.3e-271
Identity = 529/972 (54.42%), Postives = 664/972 (68.31%), Query Frame = 0

Query: 1   MAEGVLSNFAGNVLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSK 60
           MAEGVL N A +++ +LS+ A +QLGML G+KD+L  L+S+VSAI+AV+ DAEER TKS 
Sbjct: 4   MAEGVLFNLAADIIKKLSSLASQQLGMLWGVKDELTELRSSVSAIKAVVLDAEERQTKSH 63

Query: 61  LLKDWLNKLGEALYDADDVLDEISTQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRMAL 120
            LKDWL KL EALYDA+DVLDEIS +A+R     RG  AKQVRIFFS+ N +AF+Y++  
Sbjct: 64  FLKDWLEKLKEALYDAEDVLDEISCEAVR-----RGRKAKQVRIFFSRYNPIAFDYKITR 123

Query: 121 KLKNIRGRLDEISEEKNKFGLIEKSEARTR---YDGLSHQIGTHSAFIEGEVVGREDDKM 180
           ++KN+R RL+ I+ E+  F L E    +T+   +D +     THS     EV+GR+D K 
Sbjct: 124 QIKNVRERLEVIAAERKNFHLTEIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHKR 183

Query: 181 RIKSLLLDETDHNNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEFDVK 240
            IK LL D    +N+SFIAIVGM GIGKTTLA+ L+ND+Q+S+ FDLK+WVWVS  F+VK
Sbjct: 184 VIKDLLQDMNVKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFEVK 243

Query: 241 RVVEKMIESASGSKPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPLLMG 300
            VVEK+IESA+ S+PNV  M+ L  +LQK+++GKKYLLVMDDIWNESE+KW +LK LLMG
Sbjct: 244 IVVEKIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLLMG 303

Query: 301 CACGSKILITKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIEDANPKMIQL 360
            A GSKI+ITKRDS +  E+ +MT LI LKGLS++ SWSLF+KVAF   IE  NPK+IQL
Sbjct: 304 GARGSKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLIQL 363

Query: 361 GKEILKKCGGVPLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNSMTIIFELSYKHL 420
           GKEIL KCGGVPLVIRHIGRLLY   SE EWISFKN++L  V Q+ + M  I +LSY HL
Sbjct: 364 GKEILVKCGGVPLVIRHIGRLLYIEKSEEEWISFKNNQLLEVIQRDHDMASILKLSYNHL 423

Query: 421 PPHLKQCFAYSSLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCWRFF 480
           P +LKQCFAYSSL  K   I KD+L++QWVA GFVQS  G KSMED  NDYF ELCWRFF
Sbjct: 424 PSNLKQCFAYSSLLLKYRLIHKDELIRQWVAQGFVQSLNGCKSMEDTGNDYFMELCWRFF 483

Query: 481 Y-----EHQDGNNVCMHDMMRDLARMVASTGYARGNLKDED--GERIHHISLGHDIESWN 540
           Y     EH   ++V +HD+M DLARMVA   Y  GN   +   GE   HI    +I  W 
Sbjct: 484 YENFNDEHNFEDDVYIHDVMHDLARMVAGNEYVGGNQNHDHVVGESTRHILFDREIGIWE 543

Query: 541 DLPPELQKAKGLRTFHKS-----RMNHINEGVLDKLFSTFPHLRVLNMSWTEFKTVPDSL 600
            +P +L+KAKGLRTF  S     R N +NE VLD+LF  F  LRVL++ ++  K VPDS+
Sbjct: 544 HVPSKLRKAKGLRTFILSNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRVPDSI 603

Query: 601 NKLRHLRYLNLSDNRYLISLPNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRHLDLD 660
            +L HLRYL+LS N Y+ SLPNS++ LVNLQTL L  C  L +LPR  +NLVNLRHL++ 
Sbjct: 604 KRLTHLRYLDLSGN-YMKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRHLNIS 663

Query: 661 SVSKLTHTPKGIRKLTCLQTLNLFVLD--ENKQGSKLGELNELRDLRGGLYIKGLEQVRG 720
             + LTH P+G+ KLTCLQTL+ FVLD   ++ GSKL ELN L DLRG L I GLE ++ 
Sbjct: 664 DSTHLTHMPEGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLENLKF 723

Query: 721 NPSEARFANLKGKKGLRDLVLQWKPSSNEDESEDGLNDEIILEGLEPHPNVESVCIWRYK 780
            PSEA  ANLK KKGLR L L WK  S ++       DE IL GLEP+P +ES  I  Y 
Sbjct: 724 TPSEASLANLKEKKGLRHLTLIWKNLSGDEHRFQA--DEEILAGLEPNPAIESFRISGYF 783

Query: 781 GEGLPNW--MTNLVSKLTHISLAKCNRLQRLPQLDQLPTLKKLHLQELTCLQFIDKNNPG 840
           G  LPNW  +++ + KLT I +  C RLQ LPQ+  LP LK L+L  LT L+FID  +P 
Sbjct: 784 GVKLPNWLSLSSPLMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKFIDNTDPY 843

Query: 841 SSKSVFFPCLDILKLKNMPSLEGWWGGVLVGDGGSTKTRRLLPKFARLTRLYIYDCPKLG 900
           SS ++FFP L+ L L +MP+LE WW      +  S      LP F  LT L I +CPKL 
Sbjct: 844 SS-ALFFPSLESLCLMDMPNLEAWW------ERESMVEEEFLPTFPCLTILSISNCPKLA 903

Query: 901 WVPRQPSLEKVELNNVGVRMVRSI------GPSLYLEYVTLKNIEDLVSLPKKLCS--LT 946
            +P  P L  + L+ VG++M+ SI       PS  L+ + LK IEDL +L  KL     +
Sbjct: 904 TMPGHPPLTDITLDGVGLQMMSSIRSSSNSSPSSSLKSLELKGIEDLQNLSHKLRQNLNS 960

BLAST of Sed0001988 vs. NCBI nr
Match: XP_031745107.1 (putative disease resistance protein RGA4 [Cucumis sativus])

HSP 1 Score: 932.9 bits (2410), Expect = 2.2e-267
Identity = 525/995 (52.76%), Postives = 683/995 (68.64%), Query Frame = 0

Query: 1   MAEGVLSNFAGNVLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSK 60
           MAE +L   AG +L +LS++A ++LGML GLK DL +L + VS I+ VL DAE R TKS 
Sbjct: 1   MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60

Query: 61  LLKDWLNKLGEALYDADDVLDEISTQALRRQVMTRG-IHAKQVRIFFSKSNQLAFNYRMA 120
           LL++WL+KL EALYDA+DVLDE+ST+ALRR++MTR   +AKQVRIFFSKSNQ+AFNYRMA
Sbjct: 61  LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQVRIFFSKSNQIAFNYRMA 120

Query: 121 LKLKNIRGRLDEISEEKNKFGLIEKSEARTRY---DGLSHQIGTHSAFIEGEVVGREDDK 180
            ++KNI  RLD I  EK +F L E  E+RT+Y   D +     T S+  + EV+GR+DD 
Sbjct: 121 RQIKNIWERLDAIDAEKTQFHLRENCESRTQYGSFDRIMMGRETWSSSNDEEVIGRDDDI 180

Query: 181 MRIKSLLLDETDH--NNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEF 240
             +K  LLD   +  +NVSFIAI GMGGIGKTTLAK LYND ++S  FDLK+WVWVSD+F
Sbjct: 181 KEVKERLLDMNMNVTHNVSFIAIAGMGGIGKTTLAKSLYNDEEVSGFFDLKIWVWVSDQF 240

Query: 241 DVKRVVEKMIESASGSKPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPL 300
           +V+ V EKMIESA+ + P+V+ M+ L  +LQK+I  +KYLLVMDD+WNESEEKW  LK L
Sbjct: 241 EVQVVAEKMIESATKNNPSVKGMEALQAKLQKVIGERKYLLVMDDVWNESEEKWHGLKSL 300

Query: 301 LMGCACGSKILITKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIEDANPKM 360
           LMG A GSK+LITKRD KV  E+ SMT L  L+GLS+++SW LF KVAFK   E  +P  
Sbjct: 301 LMGGARGSKVLITKRDRKVATEIKSMTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPST 360

Query: 361 IQLGKEILKKCGGVPLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNSMTIIFELSY 420
           I LGKEIL +CGGVPLVIRH+GR+LYS+ S+ EW+SFK++EL  V QQ N MT I +LSY
Sbjct: 361 IHLGKEILVRCGGVPLVIRHVGRMLYSKTSQEEWMSFKDNELLEVIQQDNDMTSILKLSY 420

Query: 421 KHLPPHLKQCFAYSSLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCW 480
            HLPP+LK+CFAYSSL+ K   I+   L++QWVA GF++   G KS+ED   DYF ELCW
Sbjct: 421 NHLPPNLKRCFAYSSLFPKGYKIEIKDLIRQWVAQGFIEVSNGRKSLEDTGKDYFNELCW 480

Query: 481 RFFYEHQD-----GNNVCMHDMMRDLARMVASTG-YARGNLKDE--DGERIHHISLGHDI 540
           RFFY +        + VCMHD+M +  R VA    Y RGN  ++    E+  HIS  + I
Sbjct: 481 RFFYANSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQTLHISFDYGI 540

Query: 541 ESWNDLPPELQKAKGLRTF-----HKSRMNHINEGVLDKLFSTFPHLRVLNMSWTEFKTV 600
           +SW D+  +L KAKGLRT         +MN I++ +LD+LFS+FP LRVL++ +++   V
Sbjct: 541 QSWQDVLSKLCKAKGLRTILLLFRPYEKMNKIDKAILDELFSSFPRLRVLDLHFSQISVV 600

Query: 601 PDSLNKLRHLRYLNLSDNRYLISLPNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRH 660
           P S+ KLRHLRYL+LS+N   + +P+SI EL NLQTL L  C EL ELPRDI NLVNLRH
Sbjct: 601 PKSIKKLRHLRYLDLSENDMEL-IPHSIIELQNLQTLNLTECYELKELPRDIDNLVNLRH 660

Query: 661 LDLDSVSKLTHTPKGIRKLTCLQTLNLFVLDENKQGSKLGELNELRDLRGGLYIKGLEQV 720
           L  +   ++T T +G+ KLTCLQT++LFV D  K+ +KL ELN+L  L G L I GLE++
Sbjct: 661 LTFEPCMEVTPTSEGMEKLTCLQTISLFVFD-CKKTNKLWELNDLSYLTGELKIIGLEKL 720

Query: 721 RGNPSEARFANLKGKKGLRDLVLQWKPSSNEDESEDGLNDEIILEGLEPHPNVESVCIWR 780
           R +PSE    NLK KKG + L L+WK   +E E E    DE I+EGLEPHPNVES+ I  
Sbjct: 721 RSSPSEITLINLKDKKGWQGLNLEWKLGKDEYEGE---ADETIMEGLEPHPNVESLSING 780

Query: 781 YKGEGLPNWMTNLVSKLTHISLAKCNRLQRLPQLDQLPTLKKLHLQELTCLQFIDKNNPG 840
           Y G  LPNW+ N + KLT I +  C R+Q LPQ +QL  L+ LHL  L  L+FIDK++P 
Sbjct: 781 YTGGALPNWVFNSLMKLTEIEIENCPRVQHLPQFNQLQDLRALHLVGLRSLEFIDKSDPY 840

Query: 841 SSKSVFFPCLDILKLKNMPSLEGWW---GGVLVGDGGSTKTRRLLPKFARLTRLYIYDCP 900
           SS SVFFP L  L+L++MP+LEGWW      +V    S K + L P F ++  L IY CP
Sbjct: 841 SS-SVFFPSLKFLRLEDMPNLEGWWELGESKVVARETSGKAKWLPPTFPQVNFLRIYGCP 900

Query: 901 KLGWVPRQPSL-EKVELNNVGVRMVRSIGP-------SLY----LEYVTLKNIEDLVSLP 960
           KL  +P+  S+   V L+++GV+MV +IGP       S++    L+Y+  +  +DLVS  
Sbjct: 901 KLSSMPKLASIGADVILHDIGVQMVSTIGPVSSFMFLSMHGMTNLKYLWEEFQQDLVSSS 960

BLAST of Sed0001988 vs. NCBI nr
Match: KAE8646395.1 (hypothetical protein Csa_016171 [Cucumis sativus])

HSP 1 Score: 932.9 bits (2410), Expect = 2.2e-267
Identity = 525/995 (52.76%), Postives = 683/995 (68.64%), Query Frame = 0

Query: 1   MAEGVLSNFAGNVLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSK 60
           MAE +L   AG +L +LS++A ++LGML GLK DL +L + VS I+ VL DAE R TKS 
Sbjct: 1   MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60

Query: 61  LLKDWLNKLGEALYDADDVLDEISTQALRRQVMTRG-IHAKQVRIFFSKSNQLAFNYRMA 120
           LL++WL+KL EALYDA+DVLDE+ST+ALRR++MTR   +AKQVRIFFSKSNQ+AFNYRMA
Sbjct: 61  LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQVRIFFSKSNQIAFNYRMA 120

Query: 121 LKLKNIRGRLDEISEEKNKFGLIEKSEARTRY---DGLSHQIGTHSAFIEGEVVGREDDK 180
            ++KNI  RLD I  EK +F L E  E+RT+Y   D +     T S+  + EV+GR+DD 
Sbjct: 121 RQIKNIWERLDAIDAEKTQFHLRENCESRTQYGSFDRIMMGRETWSSSNDEEVIGRDDDI 180

Query: 181 MRIKSLLLDETDH--NNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEF 240
             +K  LLD   +  +NVSFIAI GMGGIGKTTLAK LYND ++S  FDLK+WVWVSD+F
Sbjct: 181 KEVKERLLDMNMNVTHNVSFIAIAGMGGIGKTTLAKSLYNDEEVSGFFDLKIWVWVSDQF 240

Query: 241 DVKRVVEKMIESASGSKPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPL 300
           +V+ V EKMIESA+ + P+V+ M+ L  +LQK+I  +KYLLVMDD+WNESEEKW  LK L
Sbjct: 241 EVQVVAEKMIESATKNNPSVKGMEALQAKLQKVIGERKYLLVMDDVWNESEEKWHGLKSL 300

Query: 301 LMGCACGSKILITKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIEDANPKM 360
           LMG A GSK+LITKRD KV  E+ SMT L  L+GLS+++SW LF KVAFK   E  +P  
Sbjct: 301 LMGGARGSKVLITKRDRKVATEIKSMTSLFTLEGLSESNSWLLFSKVAFKEGKESTDPST 360

Query: 361 IQLGKEILKKCGGVPLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNSMTIIFELSY 420
           I LGKEIL +CGGVPLVIRH+GR+LYS+ S+ EW+SFK++EL  V QQ N MT I +LSY
Sbjct: 361 IHLGKEILVRCGGVPLVIRHVGRMLYSKTSQEEWMSFKDNELLEVIQQDNDMTSILKLSY 420

Query: 421 KHLPPHLKQCFAYSSLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCW 480
            HLPP+LK+CFAYSSL+ K   I+   L++QWVA GF++   G KS+ED   DYF ELCW
Sbjct: 421 NHLPPNLKRCFAYSSLFPKGYKIEIKDLIRQWVAQGFIEVSNGRKSLEDTGKDYFNELCW 480

Query: 481 RFFYEHQD-----GNNVCMHDMMRDLARMVASTG-YARGNLKDE--DGERIHHISLGHDI 540
           RFFY +        + VCMHD+M +  R VA    Y RGN  ++    E+  HIS  + I
Sbjct: 481 RFFYANSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQTLHISFDYGI 540

Query: 541 ESWNDLPPELQKAKGLRTF-----HKSRMNHINEGVLDKLFSTFPHLRVLNMSWTEFKTV 600
           +SW D+  +L KAKGLRT         +MN I++ +LD+LFS+FP LRVL++ +++   V
Sbjct: 541 QSWQDVLSKLCKAKGLRTILLLFRPYEKMNKIDKAILDELFSSFPRLRVLDLHFSQISVV 600

Query: 601 PDSLNKLRHLRYLNLSDNRYLISLPNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRH 660
           P S+ KLRHLRYL+LS+N   + +P+SI EL NLQTL L  C EL ELPRDI NLVNLRH
Sbjct: 601 PKSIKKLRHLRYLDLSENDMEL-IPHSIIELQNLQTLNLTECYELKELPRDIDNLVNLRH 660

Query: 661 LDLDSVSKLTHTPKGIRKLTCLQTLNLFVLDENKQGSKLGELNELRDLRGGLYIKGLEQV 720
           L  +   ++T T +G+ KLTCLQT++LFV D  K+ +KL ELN+L  L G L I GLE++
Sbjct: 661 LTFEPCMEVTPTSEGMEKLTCLQTISLFVFD-CKKTNKLWELNDLSYLTGELKIIGLEKL 720

Query: 721 RGNPSEARFANLKGKKGLRDLVLQWKPSSNEDESEDGLNDEIILEGLEPHPNVESVCIWR 780
           R +PSE    NLK KKG + L L+WK   +E E E    DE I+EGLEPHPNVES+ I  
Sbjct: 721 RSSPSEITLINLKDKKGWQGLNLEWKLGKDEYEGE---ADETIMEGLEPHPNVESLSING 780

Query: 781 YKGEGLPNWMTNLVSKLTHISLAKCNRLQRLPQLDQLPTLKKLHLQELTCLQFIDKNNPG 840
           Y G  LPNW+ N + KLT I +  C R+Q LPQ +QL  L+ LHL  L  L+FIDK++P 
Sbjct: 781 YTGGALPNWVFNSLMKLTEIEIENCPRVQHLPQFNQLQDLRALHLVGLRSLEFIDKSDPY 840

Query: 841 SSKSVFFPCLDILKLKNMPSLEGWW---GGVLVGDGGSTKTRRLLPKFARLTRLYIYDCP 900
           SS SVFFP L  L+L++MP+LEGWW      +V    S K + L P F ++  L IY CP
Sbjct: 841 SS-SVFFPSLKFLRLEDMPNLEGWWELGESKVVARETSGKAKWLPPTFPQVNFLRIYGCP 900

Query: 901 KLGWVPRQPSL-EKVELNNVGVRMVRSIGP-------SLY----LEYVTLKNIEDLVSLP 960
           KL  +P+  S+   V L+++GV+MV +IGP       S++    L+Y+  +  +DLVS  
Sbjct: 901 KLSSMPKLASIGADVILHDIGVQMVSTIGPVSSFMFLSMHGMTNLKYLWEEFQQDLVSSS 960

BLAST of Sed0001988 vs. NCBI nr
Match: XP_038896095.1 (putative disease resistance protein RGA4 [Benincasa hispida])

HSP 1 Score: 932.9 bits (2410), Expect = 2.2e-267
Identity = 519/980 (52.96%), Postives = 666/980 (67.96%), Query Frame = 0

Query: 1   MAEGVLSNFAGNVLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSK 60
           MAEG+L N AG ++ +LS+ A +QLGML G+ DDL +L S+VSAI+AVL DAEER T S 
Sbjct: 1   MAEGILFNLAGEIIKKLSSLAFQQLGMLWGVNDDLTKLTSSVSAIKAVLLDAEERQTGSN 60

Query: 61  LLKDWLNKLGEALYDADDVLDEISTQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRMAL 120
            L+DWL KL EALYDA+DVLDEIS +AL R+VMT G  AKQVRIFFSKSN +AFNY+MA 
Sbjct: 61  SLQDWLGKLKEALYDAEDVLDEISYEALHREVMTAGKKAKQVRIFFSKSNPVAFNYKMAR 120

Query: 121 KLKNIRGRLDEISEEKNKFGLIEKSEARTRY---DGLSHQIGTHSAFIEGEVVGREDDKM 180
           ++KN+R RL  I+ E+ +F   E    + +Y   D +     T S     EV+GR+  K 
Sbjct: 121 QIKNVRERLGVIAAERKEFHFAEMFGQKFQYPLFDQIHIGAETRSKSNNEEVIGRDGHKR 180

Query: 181 RIKSLLLDETDHNNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEFDVK 240
            IK LL D    +N+SFIAIVGMGGIGKTTLA+ L+ND Q+SK+FDLK+WVWVS  F+VK
Sbjct: 181 VIKDLLQDMKVKHNISFIAIVGMGGIGKTTLARSLFNDAQVSKYFDLKIWVWVSQNFEVK 240

Query: 241 RVVEKMIESASGSKPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPLLMG 300
            V+EK+IES + S+PNVQ M+ L  +LQK+I+GKKYLLVMDDIWNESE+KW  LK LLMG
Sbjct: 241 IVMEKIIESITESRPNVQGMESLQNQLQKMIQGKKYLLVMDDIWNESEQKWFDLKNLLMG 300

Query: 301 CACGSKILITKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIEDANPKMIQL 360
            A GSKI+ITKRDS +  E+ +MT LI LKGLS+ +SWSL ++VAF   IE  NPK I++
Sbjct: 301 GARGSKIMITKRDSTLAVEISNMTNLITLKGLSECNSWSLLKRVAFMDGIEPTNPKFIKV 360

Query: 361 GKEILKKCGGVPLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNSMTIIFELSYKHL 420
           GKEI+ KCGGVPL+IRHIGRLLY+  SE EW SFKN++LSRV Q   +M  I  LSY +L
Sbjct: 361 GKEIVVKCGGVPLIIRHIGRLLYNNTSEEEWTSFKNNQLSRVIQHDQNMIPILRLSYNYL 420

Query: 421 PPHLKQCFAYSSLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCWRFF 480
           PP+LK+CFAYSSL  K+  I KD+L++QW+A GF+Q+    KSMEDI NDYF ELCWRFF
Sbjct: 421 PPNLKRCFAYSSLLLKHWPIHKDELIQQWMAQGFIQASNCGKSMEDIGNDYFMELCWRFF 480

Query: 481 YEHQ---DGNNVCMHDMMRDLARMVASTGY---ARGNLKDEDGERIHHISLGHDIESWND 540
           +EH      + + +HD+M DLA+ VA   Y   +  N  D   E+  H+S  H I+SW D
Sbjct: 481 FEHSGRVSDSYIFVHDVMHDLAKTVAGNEYVCISSKNNYDNVSEKTRHVSFDHKIKSWWD 540

Query: 541 LPPELQKAKGLRTFHKS-----RMNHINEGVLDKLFSTFPHLRVLNMSWTEFKTVPDSLN 600
             P L K +GLRTF  S      +  +N+  L+ LFS+FP LRVL +  +E K VP S+ 
Sbjct: 541 DLPTLCKGEGLRTFLPSYYPYKAVVEVNKVSLNALFSSFPRLRVLCLGDSEIKIVPKSIE 600

Query: 601 KLRHLRYLNLSDNRYLISLPNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRHLDLDS 660
           KLR LRYL+LS N  ++SLPNSIS+L +L+TL L  C  L ELPR   NLVNLRHL L  
Sbjct: 601 KLRQLRYLDLSGNENMVSLPNSISKLQHLETLNLKRCFALRELPRGTCNLVNLRHLYLPL 660

Query: 661 VSKLTHTPKGIRKLTCLQTLNLFVLDENKQ---GSKLGELNELRDLRGGLYIKGLEQVRG 720
              LT+ P+G+ KLTCLQTL+ FVLD N++    SKL ELN L+DLRG L I GL+Q+R 
Sbjct: 661 C--LTYMPEGMGKLTCLQTLSFFVLDNNREDTRSSKLSELNGLKDLRGCLTIDGLDQLRS 720

Query: 721 NPSEARFANLKGKKGLRDLVLQWKPSSNEDESED---GLNDEIILEGLEPHPNVESVCIW 780
           NP EA  ANLK KKGL++L L W  S +     D   G +D  ++EGLEP  NVE + I 
Sbjct: 721 NPKEASSANLKDKKGLQNLQLTWNLSKDVKYKADEAGGCSDVKVMEGLEPQQNVEYLSIH 780

Query: 781 RYKGEGLPNWMTNLVSKLTHISLAKCNRLQRLPQLDQLPTLKKLHLQELTCLQFIDKNNP 840
            Y G  LP+WM+  + KLT I + +C+RLQ L QL+ LP+L+++ L+ +  ++FIDKN+P
Sbjct: 781 GYSGARLPDWMSRPLLKLTSIRIQECHRLQHLTQLNYLPSLREVILRSIIPVKFIDKNDP 840

Query: 841 GSSKSVFFPCLDILKLKNMPSLEGWWGGVLVGDGGSTKTRRLLPKFARLTRLYIYDCPKL 900
             S SVFFP L  L L+NMP+L+GWW            T  L P F  LT L I +CPKL
Sbjct: 841 CPS-SVFFPSLKTLCLENMPNLKGWW--------EKESTAGLRPAFPCLTTLSISNCPKL 900

Query: 901 GWVPRQPSLEKVELNNVGVRMVRSIGPSL----------YLEYVTLKNIEDLVSLPKKLC 949
             +P  P LE ++L++VG++M+ SIG SL           L+ + LK I DL +LP KL 
Sbjct: 901 ASIPEHPCLENLKLDDVGLQMMSSIGTSLDASQSSSSSSSLKSLNLKRIGDLENLPCKLR 960

BLAST of Sed0001988 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 488.0 bits (1255), Expect = 2.5e-136
Identity = 334/987 (33.84%), Postives = 528/987 (53.50%), Query Frame = 0

Query: 1   MAEGVLSNFAGNVLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSK 60
           MAE  L     N+   +  E    LG++ G + + K+L S  S IQAVL DA+E+  K K
Sbjct: 1   MAEAFLQVLLDNLTFFIQGE----LGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYK 60

Query: 61  LLKDWLNKLGEALYDADDVLDEISTQALR-RQVMTRGIHAKQVRIFFSKSNQLAFNYRMA 120
            +K+WL KL  A Y+ DD+LD+  T+A R +Q +    H + +           F Y++ 
Sbjct: 61  AIKNWLQKLNVAAYEVDDILDDCKTEAARFKQAVLGRYHPRTI----------TFCYKVG 120

Query: 121 LKLKNIRGRLDEISEEKNKFGL----IEKSEARTRYDGLSHQIGTHSAFIEGEVVGREDD 180
            ++K +  +LD I+EE+  F L    IE+  AR +         T     E +V GRE +
Sbjct: 121 KRMKEMMEKLDAIAEERRNFHLDERIIERQAARRQ---------TGFVLTEPKVYGREKE 180

Query: 181 KMRIKSLLLDETDHN-NVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEF 240
           +  I  +L++   ++  V  + I+GMGG+GKTTLA++++ND ++++HF+LK+WV VSD+F
Sbjct: 181 EDEIVKILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDF 240

Query: 241 DVKRVVEKMIESASGSKPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPL 300
           D KR+++ ++ES  G      ++ PL  +LQ+L+ GK+Y LV+DD+WNE +EKW+ L+ +
Sbjct: 241 DEKRLIKAIVESIEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAV 300

Query: 301 LMGCACGSKILITKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIEDANPKM 360
           L   A G+ ILIT R  K+ + +G++ +L  L  LS    W LF++ AF    E  +PK+
Sbjct: 301 LKIGASGASILITTRLEKIGSIMGTL-QLYQLSNLSQEDCWLLFKQRAFCHQTE-TSPKL 360

Query: 361 IQLGKEILKKCGGVPLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNSMTIIFELSY 420
           +++GKEI+KKCGGVPL  + +G LL  +  E EW   ++ E+  + Q +NS+     LSY
Sbjct: 361 MEIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDENSVLPALRLSY 420

Query: 421 KHLPPHLKQCFAYSSLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCW 480
            HLP  L+QCFAY +++ K+  I+K+ L+  W+AH F+ S +G+  +ED+ N+ + EL  
Sbjct: 421 HHLPLDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFLLS-KGNMELEDVGNEVWNELYL 480

Query: 481 RFFYEHQDGNN----VCMHDMMRDLARMVASTGYARGNLKD---EDGERIHHISLGHDIE 540
           R F++  +  +      MHD++ DLA  + S   +  +++    +D E +  I     + 
Sbjct: 481 RSFFQEIEVKSGKTYFKMHDLIHDLATSMFSASASSRSIRQINVKDDEDMMFI-----VT 540

Query: 541 SWNDLPPELQKAKGLRTFHKSRMNHINEGVLDKLFSTFPHLRVLNMSWTEFKTVPDSLNK 600
           ++ D+                  + +       LF  F  LRVLN+S +EF+ +P S+  
Sbjct: 541 NYKDM-------------MSIGFSEVVSSYSPSLFKRFVSLRVLNLSNSEFEQLPSSVGD 600

Query: 601 LRHLRYLNLSDNRYLISLPNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRHLDLDSV 660
           L HLRYL+LS N+ + SLP  + +L NLQTL L  C+ L+ LP+    L +LR+L LD  
Sbjct: 601 LVHLRYLDLSGNK-ICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDH- 660

Query: 661 SKLTHTPKGIRKLTCLQTLNLFVLDENKQGSKLGELNELRDLRGGLYIKGLEQVRGNPSE 720
             LT  P  I  LTCL+TL  FV+ E K G +LGEL  L +LRG + I  LE+V+ N  E
Sbjct: 661 CPLTSMPPRIGLLTCLKTLGYFVVGERK-GYQLGELRNL-NLRGAISITHLERVK-NDME 720

Query: 721 ARFANLKGKKGLRDLVLQW-KPSSNEDESEDGLNDEIILEGLEPHPNVESVCIWRYKGEG 780
           A+ ANL  K  L  L + W +P+  E E      +  +LE L+PHPN++ + I  + G  
Sbjct: 721 AKEANLSAKANLHSLSMSWDRPNRYESE------EVKVLEALKPHPNLKYLEIIDFCGFC 780

Query: 781 LPNWMTNLVSK-LTHISLAKCNRLQRLPQLDQLPTLKKLHLQELTCLQFIDKNNPGSSKS 840
           LP+WM + V K +  I ++ C     LP   +LP L+ L LQ+            GS + 
Sbjct: 781 LPDWMNHSVLKNVVSILISGCENCSCLPPFGELPCLESLELQD------------GSVEV 840

Query: 841 VFFPCLDILKLKNMPSLEGWWGGVLVGDGGSTKTRRLLPKFARLTRLYIYDCPKLGWVPR 900
            +      L  +  PSL     G      G  + +    +F  L  + I DCP   + P 
Sbjct: 841 EYVEDSGFLTRRRFPSLRKLHIGGFCNLKGLQRMKG-AEQFPVLEEMKISDCPMFVF-PT 900

Query: 901 QPSLEKVEL-------------------------NNVGVRMVRSIGPSL-YLEYVTLKNI 946
             S++K+E+                         N+    ++  +  +L  L Y+++  +
Sbjct: 901 LSSVKKLEIWGEADAGGLSSISNLSTLTSLKIFSNHTVTSLLEEMFKNLENLIYLSVSFL 918

BLAST of Sed0001988 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.0e-130
Identity = 322/975 (33.03%), Postives = 530/975 (54.36%), Query Frame = 0

Query: 13  VLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSKLLKDWLNKLGEA 72
           +L  L++    +L ++ G + + ++L S  S IQAVL DA+E+  K K +++WL KL  A
Sbjct: 9   LLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKLNSA 68

Query: 73  LYDADDVLDEISTQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRMALKLKNIRGRLDEI 132
            Y+ DD+L E   +A+R          +Q R+ F     + F +++  ++K I  +LD I
Sbjct: 69  AYEVDDILGECKNEAIR---------FEQSRLGFYHPGIINFRHKIGRRMKEIMEKLDAI 128

Query: 133 SEEKNKFGLIEK-----SEARTRYDGLSHQIGTHSAFIEGEVVGREDDKMRIKSLLLDET 192
           SEE+ KF  +EK     + A TR  G            E +V GR+ ++  I  +L++  
Sbjct: 129 SEERRKFHFLEKITERQAAAATRETGF--------VLTEPKVYGRDKEEDEIVKILINNV 188

Query: 193 D-HNNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEFDVKRVVEKMIES 252
           +    +    I+GMGG+GKTTLA++++ND +++KHF+ K+WV VSD+FD KR+++ +I +
Sbjct: 189 NVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGN 248

Query: 253 ASGSKPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPLLMGCACGSKILI 312
              S P+V+++     +LQ+L+ GK+YLLV+DD+WN+  EKW +L+ +L   A G+ IL 
Sbjct: 249 IERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGARGASILA 308

Query: 313 TKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIEDANPKMIQLGKEILKKCG 372
           T R  KV + +G++ +  +L  LS   S  LF + AF    ++ANP ++ +GKEI+KKCG
Sbjct: 309 TTRLEKVGSIMGTL-QPYHLSNLSPHDSLLLFMQRAFGQQ-KEANPNLVAIGKEIVKKCG 368

Query: 373 GVPLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNSMTIIFELSYKHLPPHLKQCFA 432
           GVPL  + +G LL  +  E EW   +++E+  + Q ++S+     LSY HLP  L+QCFA
Sbjct: 369 GVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESSILPALRLSYHHLPLDLRQCFA 428

Query: 433 YSSLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCWRFFY---EHQDG 492
           Y +++ K+  + K+ L+  W+AHGF+ S +G+  +ED+ N+ + EL  R F+   E + G
Sbjct: 429 YCAVFPKDTKMIKENLITLWMAHGFLLS-KGNLELEDVGNEVWNELYLRSFFQEIEAKSG 488

Query: 493 NNVC-MHDMMRDLARMVASTGYARGNLKDEDGERIHH-ISLGHDIESWNDLPPELQKAKG 552
           N    +HD++ DLA  + S   + GN+++ + +   H +S+G      +  P  L+K   
Sbjct: 489 NTYFKIHDLIHDLATSLFSASASCGNIREINVKDYKHTVSIGFAAVVSSYSPSLLKK--- 548

Query: 553 LRTFHKSRMNHINEGVLDKLFSTFPHLRVLNMSWTEFKTVPDSLNKLRHLRYLNLSDNRY 612
                                  F  LRVLN+S+++ + +P S+  L HLRYL+LS N +
Sbjct: 549 -----------------------FVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCNNF 608

Query: 613 LISLPNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRHLDLDSVSKLTHTPKGIRKLT 672
             SLP  + +L NLQTL ++ C  L  LP+    L +LRHL +D    LT TP  I  LT
Sbjct: 609 R-SLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDG-CPLTSTPPRIGLLT 668

Query: 673 CLQTLNLFVLDENKQGSKLGELNELRDLRGGLYIKGLEQVRGNPSEARFANLKGKKGLRD 732
           CL+TL  F++  +K+G +LGEL  L +L G + I  LE+V+ N ++A  ANL  K  L+ 
Sbjct: 669 CLKTLGFFIVG-SKKGYQLGELKNL-NLCGSISITHLERVK-NDTDAE-ANLSAKANLQS 728

Query: 733 LVLQWKPSSNEDESEDGLNDEIILEGLEPHPNVESVCIWRYKGEGLPNWMT-NLVSKLTH 792
           L + W    N+  +     +  +LE L+PHPN++ + I  + G   P+W+  +++ K+  
Sbjct: 729 LSMSW---DNDGPNRYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVIS 788

Query: 793 ISLAKCNRLQRLPQLDQLPTLKKLHLQELTC-LQFIDKNNPGSSKSV--FFPCLDILKLK 852
           + +  C     LP   +LP L+ L LQ  +  ++++++++  S  S    FP L  L++ 
Sbjct: 789 VRIKSCKNCLCLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIW 848

Query: 853 NMPSLEGWWGGVLVGDGGSTKTRRLLPKFARLTRLYIYDCPKLGWVPRQPSLEKVELN-N 912
              SL+G     L+ + G         KF  L  + I  CP L   P   S++K+E++ N
Sbjct: 849 FFRSLKG-----LMKEEGE-------EKFPMLEEMAILYCP-LFVFPTLSSVKKLEVHGN 908

Query: 913 VGVRMVRSIG-------------------------PSLYLEYVTLKNIEDLVSLPKKLCS 946
              R + SI                              LE+++  + ++L  LP  L S
Sbjct: 909 TNTRGLSSISNLSTLTSLRIGANYRATSLPEEMFTSLTNLEFLSFFDFKNLKDLPTSLTS 915

BLAST of Sed0001988 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 3.8e-129
Identity = 325/967 (33.61%), Postives = 518/967 (53.57%), Query Frame = 0

Query: 1   MAEGVLSNFAGNVLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSK 60
           MAE  +     N+ + L  E    L +L G +D+ +RL S  S IQAVL DA+E+   +K
Sbjct: 1   MAEAFIQVLLDNLTSFLKGE----LVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNK 60

Query: 61  LLKDWLNKLGEALYDADDVLDEISTQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRMAL 120
            L++WL KL  A Y+ DD+LDE  T+A R      G +  +V         + F +++  
Sbjct: 61  PLENWLQKLNAATYEVDDILDEYKTKATRFSQSEYGRYHPKV---------IPFRHKVGK 120

Query: 121 KLKNIRGRLDEISEEKNKFGLIEKSEARTRYDGLSHQIGTHSAFIEGEVVGREDDKMRIK 180
           ++  +  +L  I+EE+  F L EK   R      + +  T S   E +V GR+ +K  I 
Sbjct: 121 RMDQVMKKLKAIAEERKNFHLHEKIVERQ-----AVRRETGSVLTEPQVYGRDKEKDEIV 180

Query: 181 SLLLDE-TDHNNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEFDVKRV 240
            +L++  +D  ++S + I+GMGG+GKTTLA++++ND ++++HF  K+W+ VS++FD KR+
Sbjct: 181 KILINNVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRL 240

Query: 241 VEKMIESASGSKPNVQEMD--PLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPLLMG 300
           ++ ++ES  G +P + EMD  PL  +LQ+L+ GK+YLLV+DD+WNE ++KW  L+ +L  
Sbjct: 241 IKAIVESIEG-RPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLKV 300

Query: 301 CACGSKILITKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIEDANPKMIQL 360
            A G+ +L T R  KV + +G++ +   L  LS    W LF + AF    E+ NP ++ +
Sbjct: 301 GASGASVLTTTRLEKVGSIMGTL-QPYELSNLSQEDCWLLFMQRAFGHQ-EEINPNLVAI 360

Query: 361 GKEILKKCGGVPLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNSMTIIFELSYKHL 420
           GKEI+KK GGVPL  + +G +L  +  E  W   ++  +  + Q ++S+     LSY  L
Sbjct: 361 GKEIVKKSGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESSILPALRLSYHQL 420

Query: 421 PPHLKQCFAYSSLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCWRFF 480
           P  LKQCFAY +++ K+  ++K++L+  W+AHGF+ S +G+  +ED+ ++ + EL  R F
Sbjct: 421 PLDLKQCFAYCAVFPKDAKMEKEKLISLWMAHGFLLS-KGNMELEDVGDEVWKELYLRSF 480

Query: 481 Y---EHQDGNNVC-MHDMMRDLARMVASTGYARGNLKD-EDGERIHHISLGH-DIESWND 540
           +   E +DG     MHD++ DLA  + S   +  N+++       H +S+G  ++  +  
Sbjct: 481 FQEIEVKDGKTYFKMHDLIHDLATSLFSANTSSSNIREINKHSYTHMMSIGFAEVVFFYT 540

Query: 541 LPPELQKAKGLRTFHKSRMNHINEGVLDKLFSTFPHLRVLNMSWTEFKTVPDSLNKLRHL 600
           LPP                       L+K  S    LRVLN+  + F  +P S+  L HL
Sbjct: 541 LPP-----------------------LEKFIS----LRVLNLGDSTFNKLPSSIGDLVHL 600

Query: 601 RYLNLSDNRYLISLPNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRHLDLDSVSKLT 660
           RYLNL  +  + SLP  + +L NLQTL L  C +L  LP++   L +LR+L LD    LT
Sbjct: 601 RYLNLYGSG-MRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLT 660

Query: 661 HTPKGIRKLTCLQTLNLFVLDENKQGSKLGELNELRDLRGGLYIKGLEQVRGNPSEARFA 720
             P  I  LTCL+TL  FV+   K+G +LGEL  L +L G + I  LE+V+ N  +A+ A
Sbjct: 661 CMPPRIGSLTCLKTLGQFVVG-RKKGYQLGELGNL-NLYGSIKISHLERVK-NDKDAKEA 720

Query: 721 NLKGKKGLRDLVLQWK---PSSNEDESEDGLNDEIILEGLEPHPNVESVCIWRYKGEGLP 780
           NL  K  L  L + W    P   E E      +  +LE L+PH N+ S+ I+ ++G  LP
Sbjct: 721 NLSAKGNLHSLSMSWNNFGPHIYESE------EVKVLEALKPHSNLTSLKIYGFRGIHLP 780

Query: 781 NWMTNLVSK-LTHISLAKCNRLQRLPQLDQLPTLKKLHLQ----ELTCLQFIDKN-NPGS 840
            WM + V K +  I ++       LP    LP L+ L L     ++  ++ +D + + G 
Sbjct: 781 EWMNHSVLKNIVSILISNFRNCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGF 840

Query: 841 SKSVFFPCLDILKLKNMPSLEGWWGGVLVGDGGSTKTRRLLPKFARLTRLYIYDCPKLGW 900
              + FP L  L + +  SL+    G+L  +G          +F  L  + I++CP L  
Sbjct: 841 PTRIRFPSLRKLDIWDFGSLK----GLLKKEGEE--------QFPVLEEMIIHECPFLTL 896

Query: 901 VPRQPSLEKVEL--NNVGVRMVRSIGPSL-YLEYVTLKNIEDLVSLPKKLCSL-TIRKLN 946
                +L  + +  N V       +  +L  L+Y+T+    +L  LP  L SL  ++ L 
Sbjct: 901 SSNLRALTSLRICYNKVATSFPEEMFKNLANLKYLTISRCNNLKELPTSLASLNALKSLK 896

BLAST of Sed0001988 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 5.7e-125
Identity = 298/899 (33.15%), Postives = 481/899 (53.50%), Query Frame = 0

Query: 35  LKRLKSNVSAIQAVLTDAEERSTKSKLLKDWLNKLGEALYDADDVLDEISTQALRRQVMT 94
           L+RL + +  I AVL DAEE+   + +++ W+N+L + +Y A+D LD+I+T+ALR  +  
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98

Query: 95  RGIHAKQVRIFFSKSNQLAF----NYRMALKLKNIRGRLDEISEEKNKFGLIEKSEARTR 154
               + ++R    + +   F    +  +  +L+ +  RL+ ++ ++N  GL E +    +
Sbjct: 99  ESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMIPK 158

Query: 155 YDGLSHQIGTHSAFIEGEVVGREDDKMRIKSLLLDETDHNN-VSFIAIVGMGGIGKTTLA 214
                 ++ T S   E EV GR+DDK  I   L+ E   +N ++ +AIVG+GG+GKTTL+
Sbjct: 159 -----QRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLS 218

Query: 215 KLLYNDTQLSKHFDLKMWVWVSDEFDVKRVVEKMIESASGSKPNVQEMDPLGGELQKLIE 274
           +LLYND  +  +F  K+W  VS+EFDV ++ +K+ ES +       ++D L  +L++ + 
Sbjct: 219 QLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLT 278

Query: 275 GK--KYLLVMDDIWNESEEKWEQLKPLLMGCACGSKILITKRDSKVPAEVGSMTKLINLK 334
           G    +LLV+DD+WNE+   W+ L+   +  A GS+IL+T R  +V A +     + NL+
Sbjct: 279 GTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRV-ASIMCAVHVHNLQ 338

Query: 335 GLSDASSWSLFQKVAFKVDIEDANPKMIQLGKEILKKCGGVPLVIRHIGRLLYSRFSELE 394
            LSD   WSLF K  F       N ++  L + I+ KC G+PL ++ +G +L      +E
Sbjct: 339 PLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIE 398

Query: 395 WISFKNDELSRVTQQKNSMTIIFELSYKHLPPHLKQCFAYSSLYTKNEYIDKDQLLKQWV 454
           W    +  +  +   K+++  +  +SY +LP HLK+CFAY S++ K    +KD+++  W+
Sbjct: 399 WERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWM 458

Query: 455 AHGFVQSHEGSKSMEDIANDYFTELCWRFFYEHQDGNNVCMHDMMRDLARMVASTGYARG 514
           A GF+Q    SK++E++ N+YF+EL  R   + +      MHD + +LA+  +       
Sbjct: 459 AEGFLQQTRSSKNLEELGNEYFSELESRSLLQ-KTKTRYIMHDFINELAQFAS----GEF 518

Query: 515 NLKDEDG------ERIHHISLGHDIESWNDLPPELQKAKGLRTF------HKSRMNHINE 574
           + K EDG      ER  ++S   D  +       L++ K LRTF      + SR   +++
Sbjct: 519 SSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSCCLDQ 578

Query: 575 GVLDKLFSTFPHLRVLNMSWTEF-KTVPDSLNKLRHLRYLNLSDNRYLISLPNSISELVN 634
            V +KL  T   LRVL++S  +  +  PD    + H R+L+LS    L  LP S+  + N
Sbjct: 579 MVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTE-LEKLPKSLCYMYN 638

Query: 635 LQTLCLNGCRELTELPRDIKNLVNLRHLDLDSVSKLTHTPKGIRKLTCLQTLNLFVLDEN 694
           LQTL L+ C  L ELP DI NL+NLR+LDL   +KL   P+   +L  LQTL  F +  +
Sbjct: 639 LQTLLLSYCSSLKELPTDISNLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFVSAS 698

Query: 695 KQGSKLGELNELRDLRGGLYIKGLEQVRGNPSEARFANLKGKKGLRDLVLQWKPSSNEDE 754
             GS++ EL  L DL G L I  L++V  + ++A  ANL  KK LR++   W+  S+  E
Sbjct: 699 -DGSRISELGGLHDLHGKLKIVELQRV-VDVADAAEANLNSKKHLREIDFVWRTGSSSSE 758

Query: 755 SEDG----LNDEIILEGLEPHPNVESVCIWRYKGEGLPNWMTN-LVSKLTHISLAKCNRL 814
           +        N+  + E L PH ++E + I RYKG   P+W+++   S++  I L +C   
Sbjct: 759 NNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYC 818

Query: 815 QRLPQLDQLPTLKKLHLQELTCLQFIDKNNPGSSKSVF------FPCLDILKLKNMPSLE 874
             LP L QLP LK+LH+  +  LQ I +    S + +       F  L+ L+  N+P  +
Sbjct: 819 TSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQ 878

Query: 875 GWWG-GVLVGDGGSTKTRRLLPKFARLTRLYIYDCPKL-GWVPR-QPSLEKVELNNVGV 900
            W    V  GD            F  L +L+I  CP+L G +P   PSL  + +   G+
Sbjct: 879 EWLDVRVTRGD-----------LFPSLKKLFILRCPELTGTLPTFLPSLISLHIYKCGL 911

BLAST of Sed0001988 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 447.2 bits (1149), Expect = 4.9e-124
Identity = 313/967 (32.37%), Postives = 505/967 (52.22%), Query Frame = 0

Query: 13  VLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSKLLKDWLNKLGEA 72
           VL  L++    +L +L G +D+ +RL S  S IQAVL DA+E+    K L++WL KL  A
Sbjct: 9   VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68

Query: 73  LYDADDVLDEISTQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRMALKLKNIRGRLDEI 132
            Y+ DD+LDE  T+A R      G +  +V         + F +++  ++  +  +L+ I
Sbjct: 69  TYEVDDILDEYKTKATRFLQSEYGRYHPKV---------IPFRHKVGKRMDQVMKKLNAI 128

Query: 133 SEEKNKFGLIEK---SEARTRYDGLSHQIGTHSAFIEGEVVGREDDKMRIKSLLLD-ETD 192
           +EE+ KF L EK    +A TR  G        S   E +V GR+ +K  I  +L++  +D
Sbjct: 129 AEERKKFHLQEKIIERQAATRETG--------SVLTEPQVYGRDKEKDEIVKILINTASD 188

Query: 193 HNNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEFDVKRVVEKMIESAS 252
              +S + I+GMGG+GKTTL+++++ND ++++ F  K+W+ +SD+F+ KR+++ ++ES  
Sbjct: 189 AQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIE 248

Query: 253 GSKPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPLLMGCACGSKILITK 312
           G   +  ++ PL  +LQ+L+ GK+Y LV+DD+WNE + KW  L+ +L   A G+ +L T 
Sbjct: 249 GKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGASGAFVLTTT 308

Query: 313 RDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIEDANPKMIQLGKEILKKCGGV 372
           R  KV + +G++ +   L  LS    W LF + AF    E+ NP ++ +GKEI+KKCGGV
Sbjct: 309 RLEKVGSIMGTL-QPYELSNLSPEDCWFLFMQRAFGHQ-EEINPNLMAIGKEIVKKCGGV 368

Query: 373 PLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNSMTIIFELSYKHLPPHLKQCFAYS 432
           PL  + +G +L  +  E EW   ++  +  + Q ++S+     LSY HLP  L+QCF Y 
Sbjct: 369 PLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESSILPALRLSYHHLPLDLRQCFVYC 428

Query: 433 SLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCWRFFY---EHQDGNN 492
           +++ K+  + K+ L+  W+AHGF+ S +G+  +ED+ N+ + EL  R F+   E + G  
Sbjct: 429 AVFPKDTKMAKENLIAFWMAHGFLLS-KGNLELEDVGNEVWNELYLRSFFQEIEVESGKT 488

Query: 493 VC-MHDMMRDLARMVASTGYARGNLKDEDGE---RIHHISLGHDIESWNDLPPELQKAKG 552
              MHD++ DLA  + S   +  N+++ +      +  I     + S++  P  LQK   
Sbjct: 489 YFKMHDLIHDLATSLFSANTSSSNIREINANYDGYMMSIGFAEVVSSYS--PSLLQK--- 548

Query: 553 LRTFHKSRMNHINEGVLDKLFSTFPHLRVLNMSWTEFKTVPDSLNKLRHLRYLNLSDNRY 612
                                  F  LRVLN+  +    +P S+  L HLRYL+LS N  
Sbjct: 549 -----------------------FVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFR 608

Query: 613 LISLPNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRHLDLDSVSKLTHTPKGIRKLT 672
           + +LP  + +L NLQTL L+ C  L+ LP+    L +LR+L LD  S LT TP  I  LT
Sbjct: 609 IRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCS-LTSTPPRIGLLT 668

Query: 673 CLQTLNLFVLDENKQGSKLGELNELRDLRGGLYIKGLEQVRGNPSEARFANLKGKKGLRD 732
           CL++L+ FV+ + K G +LGEL  L +L G + I  L++V+   ++A+ ANL  K  L  
Sbjct: 669 CLKSLSCFVIGKRK-GHQLGELKNL-NLYGSISITKLDRVK-KDTDAKEANLSAKANLHS 728

Query: 733 LVLQWKPSSNEDESEDGLNDEIILEGLEPHPNVESVCIWRYKGEGLPNWMTNLVSK-LTH 792
           L L W      D       D  +LE L+PH N++ + I  + G  LP+WM   V K +  
Sbjct: 729 LCLSW------DLDGKHRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVS 788

Query: 793 ISLAKCNRLQRLPQLDQLPTLKKLHLQELTC-LQFIDKN-NPGSSKSVFFPCL------D 852
           I +  C     LP   +LP L+ L L   +  +++++ N +PG      FP L      D
Sbjct: 789 IRIRGCENCSCLPPFGELPCLESLELHTGSADVEYVEDNVHPGR-----FPSLRKLVIWD 848

Query: 853 ILKLKNMPSLEG-------------WWGGVLVGDGGSTKTRRLLPKFARLTRLYIYDCPK 912
              LK +  +EG             W    ++    S KT +++   A + R        
Sbjct: 849 FSNLKGLLKMEGEKQFPVLEEMTFYWCPMFVIPTLSSVKTLKVIVTDATVLR----SISN 905

Query: 913 LGWVPRQPSLEKVELNNVGVRMVRSIGPSLYLEYVTLKNIEDLVSLPKKLCSL-TIRKLN 946
           L  +      + VE  ++   M +S+    YL+    +N+++   LP  L SL  ++ L 
Sbjct: 909 LRALTSLDISDNVEATSLPEEMFKSLANLKYLKISFFRNLKE---LPTSLASLNALKSLK 905

BLAST of Sed0001988 vs. ExPASy TrEMBL
Match: A0A6J1GPK9 (putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111456321 PE=4 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 4.2e-272
Identity = 532/972 (54.73%), Postives = 663/972 (68.21%), Query Frame = 0

Query: 1   MAEGVLSNFAGNVLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSK 60
           MAEGVL N A +++ +LS+ A +QLGML G+KD+L  LKS+VSAI+AV+ DAEER TKS 
Sbjct: 1   MAEGVLFNLAADIIKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEERQTKSH 60

Query: 61  LLKDWLNKLGEALYDADDVLDEISTQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRMAL 120
            LKDWL KL EALYDA+DVLDEIS +A+R     RG  AKQVRIFFS+ N +AF+Y+M  
Sbjct: 61  FLKDWLEKLKEALYDAEDVLDEISGEAVR-----RGRKAKQVRIFFSRYNPIAFDYKMTR 120

Query: 121 KLKNIRGRLDEISEEKNKFGLIEKSEARTR---YDGLSHQIGTHSAFIEGEVVGREDDKM 180
           ++KN+R RL  I+ E+  F L E    +T+   +D +     THS     EV+GR+D K 
Sbjct: 121 QIKNVRERLGVIAAERKNFHLTEIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHKR 180

Query: 181 RIKSLLLDETDHNNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEFDVK 240
            IK LL D    +N+SFIAIVGM GIGKTTLA+ L+ND+Q+S+ FDLK+WVWVS  F+VK
Sbjct: 181 VIKDLLQDMNVKDNISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFEVK 240

Query: 241 RVVEKMIESASGSKPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPLLMG 300
            VVEK+IESA+ S+PNV  M+ L  +LQK+++GKKYLLVMDDIWNESE+KW +LK LLMG
Sbjct: 241 IVVEKIIESATNSRPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLLMG 300

Query: 301 CACGSKILITKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIEDANPKMIQL 360
            A GSKI+ITKRDS +  E+ +MT LI LKGLS++ SWSLF+KVAF   IE  NPK+IQL
Sbjct: 301 GARGSKIMITKRDSTLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLIQL 360

Query: 361 GKEILKKCGGVPLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNSMTIIFELSYKHL 420
           GKEIL KCGGVPLVIRHIGRLLY   SE EWISFKN++L  V Q+ + M  I +LSY HL
Sbjct: 361 GKEILVKCGGVPLVIRHIGRLLYIEKSEEEWISFKNNQLLEVIQRDHDMASILKLSYNHL 420

Query: 421 PPHLKQCFAYSSLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCWRFF 480
           P +LKQCFAYSSL  K   I KD+L++QWVA GFVQS  G KSMED  NDYF ELCWRFF
Sbjct: 421 PSNLKQCFAYSSLLLKYRLIHKDELIRQWVAQGFVQSLNGCKSMEDTGNDYFMELCWRFF 480

Query: 481 Y-----EHQDGNNVCMHDMMRDLARMVASTGYARGNLKDED--GERIHHISLGHDIESWN 540
           Y     EH   ++V +HD+M DLARMVA   Y  GN   +   GE   HI    +I  W 
Sbjct: 481 YENFNDEHNFEDDVYIHDVMHDLARMVAGNEYVGGNQNHDHVVGESTRHILFDREIGIWE 540

Query: 541 DLPPELQKAKGLRTFHKS-----RMNHINEGVLDKLFSTFPHLRVLNMSWTEFKTVPDSL 600
            +P +L+KAKGLRTF  S     R N +NE VLD+LF  F  LRVL++ ++  K VPDS+
Sbjct: 541 HVPSKLRKAKGLRTFILSNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRVPDSI 600

Query: 601 NKLRHLRYLNLSDNRYLISLPNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRHLDLD 660
            +L HLRYL+LS N Y+ SLPNS++ LVNLQTL L  C  L +LPR  +NLVNLRHL++ 
Sbjct: 601 KRLTHLRYLDLSGN-YMKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRHLNIS 660

Query: 661 SVSKLTHTPKGIRKLTCLQTLNLFVLD--ENKQGSKLGELNELRDLRGGLYIKGLEQVRG 720
             + LTH P+G+ KLTCLQTL+ FVLD   ++ GSKL ELN L DLRG L I GLE ++ 
Sbjct: 661 DSTHLTHMPEGMGKLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLENLKF 720

Query: 721 NPSEARFANLKGKKGLRDLVLQWKPSSNEDESEDGLNDEIILEGLEPHPNVESVCIWRYK 780
            PSEA  ANLK KKGLR L L WK  S ++       DE IL GLEP+P +ES  I  Y 
Sbjct: 721 TPSEASLANLKEKKGLRHLTLIWKNLSGDEHRFQA--DEEILAGLEPNPAIESFRISGYF 780

Query: 781 GEGLPNW--MTNLVSKLTHISLAKCNRLQRLPQLDQLPTLKKLHLQELTCLQFIDKNNPG 840
           G  LPNW  +++ + KLT I +  C RLQ LPQ+  LP LK L+L  LT L+FID  +P 
Sbjct: 781 GVKLPNWLSLSSPLMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKFIDNTDPY 840

Query: 841 SSKSVFFPCLDILKLKNMPSLEGWWGGVLVGDGGSTKTRRLLPKFARLTRLYIYDCPKLG 900
           SS ++FFP L+ L L +MP+LE WW      +  S      LP F  LT L I +CPKL 
Sbjct: 841 SS-ALFFPSLESLCLMDMPNLEAWW------ERESMVEEEFLPTFPCLTILSISNCPKLA 900

Query: 901 WVPRQPSLEKVELNNVGVRMVRSI------GPSLYLEYVTLKNIEDLVSLPKKLCS--LT 946
            +P  P L  + L+ VG++M+ SI       PS  L+ + LK IEDL +L  KL     +
Sbjct: 901 TMPGHPPLTDITLDGVGLQMMSSIRSSSNTSPSSSLKSLELKGIEDLKNLSHKLRQNLNS 957

BLAST of Sed0001988 vs. ExPASy TrEMBL
Match: A0A6J1JVS7 (putative disease resistance protein RGA3 OS=Cucurbita maxima OX=3661 GN=LOC111488319 PE=4 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 1.0e-262
Identity = 520/959 (54.22%), Postives = 651/959 (67.88%), Query Frame = 0

Query: 14   LTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSKLLKDWLNKLGEAL 73
            L +LS+ A +QLGML G+KD+L  LKS+VSAI+AV+ DAEE+ TKS  LKDWL KL EAL
Sbjct: 233  LKKLSSLASQQLGMLWGVKDELTELKSSVSAIKAVVLDAEEQ-TKSHFLKDWLEKLKEAL 292

Query: 74   YDADDVLDEISTQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRMALKLKNIRGRLDEIS 133
            YDA+DVLDEIS +A+R     RG  AKQVRIFFS+ N +AF+Y+M  ++KN+R RL+ I+
Sbjct: 293  YDAEDVLDEISCEAVR-----RGRKAKQVRIFFSRYNPIAFDYKMTCQIKNVRERLEVIA 352

Query: 134  EEKNKFGLIEKSEARTR---YDGLSHQIGTHSAFIEGEVVGREDDKMRIKSLLLDETDHN 193
             E+  F L E    +T+   +D +     THS     EV+GR+D K  IK LL D    +
Sbjct: 353  AERKNFHLTEIIGQKTQHGSFDQIHMGAETHSFSNNEEVIGRDDHKRVIKDLLQDMNVKD 412

Query: 194  NVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEFDVKRVVEKMIESASGS 253
            N+SFIAIVGM GIGKTTLA+ L+ND+Q+S+ FDLK+WVWVS  F+VK VVEK+IESA+ S
Sbjct: 413  NISFIAIVGMSGIGKTTLARSLFNDSQVSQCFDLKIWVWVSQNFEVKIVVEKIIESATNS 472

Query: 254  KPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPLLMGCACGSKILITKRD 313
            +PNV  M+ L  +LQK+++GKKYLLVMDDIWNESE+KW +LK LLMG A GSKI+ITKRD
Sbjct: 473  RPNVHGMESLQTQLQKMLQGKKYLLVMDDIWNESEQKWFELKNLLMGGARGSKIMITKRD 532

Query: 314  SKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIEDANPKMIQLGKEILKKCGGVPL 373
            S +  E+ +MT LI LKGLS++ SWSLF+KVAF   IE  NPK+IQLGKEIL KCGGVPL
Sbjct: 533  STLAVEISNMTSLITLKGLSESDSWSLFKKVAFMDGIEPENPKLIQLGKEILVKCGGVPL 592

Query: 374  VIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNSMTIIFELSYKHLPPHLKQCFAYSSL 433
            VIRHIGRLLY + SE EWISFKN++L  V Q+ + M  I +LSY HL  +LKQCFAYSSL
Sbjct: 593  VIRHIGRLLYIQKSEEEWISFKNNQLLEVIQRDHDMASILKLSYNHLSSNLKQCFAYSSL 652

Query: 434  YTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCWRFFY-----EHQDGNN 493
              K   I KD+L++QWVA GFVQS  G KSMED ANDYF ELCWRFFY     EH   ++
Sbjct: 653  LLKYRLIHKDELIRQWVAQGFVQSLSGCKSMEDTANDYFMELCWRFFYENFNDEHNFEDD 712

Query: 494  VCMHDMMRDLARMVASTGYARGNLKDED--GERIHHISLGHDIESWNDLPPELQKAKGLR 553
            V +HD+M DLARMVA   Y  GN   +    E   HI    +I  W  +P +L KAKGLR
Sbjct: 713  VYIHDVMHDLARMVAGNEYVGGNQNHDHVVAESTRHILFDREIGIWEQVPSKLLKAKGLR 772

Query: 554  TFHKS-----RMNHINEGVLDKLFSTFPHLRVLNMSWTEFKTVPDSLNKLRHLRYLNLSD 613
            TF  S     R N +NE VLD+LF  F  LRVL++ ++  K VPDS+ +L HLRYL+LS 
Sbjct: 773  TFILSNDILDRKNQMNEVVLDELFCRFSRLRVLDLKYSNIKRVPDSIKRLTHLRYLDLSG 832

Query: 614  NRYLISLPNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRHLDLDSVSKLTHTPKGIR 673
            N Y+ SLPNS++ LVNLQTL L  C  L +LPR  +NLVNLRHL++   + LTH P+G+ 
Sbjct: 833  N-YMKSLPNSVTALVNLQTLNLKQCYALKKLPRGTRNLVNLRHLNISDSTHLTHMPEGMG 892

Query: 674  KLTCLQTLNLFVLD--ENKQGSKLGELNELRDLRGGLYIKGLEQVRGNPSEARFANLKGK 733
            KLTCLQTL+ FVLD   ++ GSKL ELN L DLRG L I GLE ++  PSEA  ANLK K
Sbjct: 893  KLTCLQTLSSFVLDSHSHRLGSKLIELNGLNDLRGSLEIIGLENLKFTPSEASLANLKEK 952

Query: 734  KGLRDLVLQWKPSSNEDESEDGLNDEIILEGLEPHPNVESVCIWRYKGEGLPNW--MTNL 793
            KGLR L L WK  S ++       DE IL GLEP+P +ES  I  Y G  LPNW  +++ 
Sbjct: 953  KGLRHLKLIWKNLSGDEHRFQA--DEEILAGLEPNPAIESFRISGYFGVKLPNWLSLSSP 1012

Query: 794  VSKLTHISLAKCNRLQRLPQLDQLPTLKKLHLQELTCLQFIDKNNPGSSKSVFFPCLDIL 853
            + KLT I +  C RLQ LPQ+  LP LK L+L  LT L+FID  +P SS ++FFP L+ L
Sbjct: 1013 LMKLTRIMIVNCKRLQNLPQIQHLPALKSLYLVNLTSLKFIDNTDPYSS-ALFFPSLESL 1072

Query: 854  KLKNMPSLEGWWGGVLVGDGGSTKTRRLLPKFARLTRLYIYDCPKLGWVPRQPSLEKVEL 913
             L +M +LE WW      +  S      LP F  LT L I +CPKL  +P  P L  + L
Sbjct: 1073 CLMDMLNLEAWW------ERESMVEEESLPTFPCLTILSISNCPKLATMPGHPPLTDITL 1132

Query: 914  NNVGVRMVRS------IGPSLYLEYVTLKNIEDLVSLPKKLCS--LTIRKLNISNCPSL 946
            + VG++M+ S        PS  L+ + LK IEDL +L  KL     +++ L I+ C +L
Sbjct: 1133 DGVGLQMMSSRRSSSNTSPSSSLKSLELKGIEDLKNLSHKLRQNLNSLKFLYITECENL 1175

BLAST of Sed0001988 vs. ExPASy TrEMBL
Match: A0A6J1D168 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111016078 PE=4 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 6.3e-252
Identity = 489/954 (51.26%), Postives = 642/954 (67.30%), Query Frame = 0

Query: 1   MAEGVLSNFAGNVLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEE--RSTK 60
           MAEGVL + AG +L +L + A ++LGM+ G+++DL +L S VS I+ VL DAEE  ++  
Sbjct: 1   MAEGVLFHVAGEILMKLGSIASQRLGMVRGVENDLTKLTSTVSVIKNVLLDAEEHCQTKT 60

Query: 61  SKLLKDWLNKLGEALYDADDVLDEISTQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRM 120
              L+DWL KL EALYDA+DVLDE+S++ L R+V+T+G +AKQVRIFFS SNQ+AFNYR+
Sbjct: 61  DHTLQDWLQKLEEALYDAEDVLDELSSETLHREVVTKGKNAKQVRIFFSGSNQIAFNYRV 120

Query: 121 ALKLKNIRGRLDEISEEKNKFGLIEKSEARTRYDGLSH----QIGTHSAFIEGEVVGRED 180
           A  +K I  RLD I  E+ +F L E  + RT Y         +  T S     EV+GR+ 
Sbjct: 121 ARNIKKIWERLDAIDAERKQFNLTENCQQRTLYGSFDQIMMGRKETCSFPNVEEVIGRDY 180

Query: 181 DKMRIKSLLLD--ETDHNNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSD 240
           DK ++K LLLD      +NVSFI I GMGGIGKTTLAK LY+D ++S+ FD  +W+W+SD
Sbjct: 181 DKKKLKDLLLDINMNVRHNVSFIVIAGMGGIGKTTLAKSLYHDREVSECFDSTIWIWISD 240

Query: 241 EFDVKRVVEKMIESASGSKPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLK 300
           +F+V+ VVEK+IESA+  KPNV+ M+ L  ELQ++I GKKYLLVMDD+W E+EEKW +LK
Sbjct: 241 QFEVRVVVEKIIESATKRKPNVEGMEALNSELQEVIGGKKYLLVMDDVWKETEEKWHELK 300

Query: 301 PLLMGCACGSKILITKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIEDANP 360
           PLLMG A GSKILITKRDSKV  E+ SMT L  LKGL +++SWSLF+KVAFK   + AN 
Sbjct: 301 PLLMGGASGSKILITKRDSKVATEIESMTSLFTLKGLQESNSWSLFRKVAFKEAKDPANS 360

Query: 361 KMIQLGKEILKKCGGVPLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQ---KNSMTII 420
            +I+LGKEIL KCGGVPLVIRHIGRLLYS+ SE EW+SFKN+EL +V QQ    + +T I
Sbjct: 361 NLIKLGKEILAKCGGVPLVIRHIGRLLYSKTSEEEWMSFKNNELLKVIQQTDKNDGLTPI 420

Query: 421 FELSYKHLPPHLKQCFAYSSLYTKNEYIDKDQLLKQWVAHGFVQSHEG-SKSMEDIANDY 480
             LSY HLPP+LKQCFAY SL+ K   ++   L++QW+AHGF++S  G + SME++   Y
Sbjct: 421 LNLSYNHLPPNLKQCFAYLSLFPKGTRLEIKDLIRQWMAHGFIESSNGTANSMEEVGKSY 480

Query: 481 FTELCWRFFYEHQD-----GNNVCMHDMMRDLARMVASTGYARGNLKDE--DGERIHHIS 540
           F ELCWRFF+E+        ++V MHD+MR++A  VA   Y  GNL  +    E+  HI 
Sbjct: 481 FKELCWRFFFENSSDECNFDDDVDMHDVMREVAMNVAGEKYVHGNLNCDFVVSEKTRHIL 540

Query: 541 LGHDIESWNDLPPELQKAKGLRTF-------HKSRMNHINEGVLDKLFSTFPHLRVLNMS 600
               I+ W D+  +LQKAKGLRTF        K+R+    E VL++L S+FP LRVL ++
Sbjct: 541 FDSKIKLWQDVLLKLQKAKGLRTFLFLSYFPFKNRLEDNIEAVLEELCSSFPRLRVLGLN 600

Query: 601 WTEFKTVPDSLNKLRHLRYLNLSDNRYLISLPNSISELVNLQTLCLNGCRELTELPRDIK 660
            ++   VP+S+  LRHLRYLNLS N Y+ SLP SI++L NLQ+L L  C +L ELPRD  
Sbjct: 601 HSKIHVVPNSIKNLRHLRYLNLSRN-YMESLPTSITKLQNLQSLDLKDCYKLNELPRDTS 660

Query: 661 NLVNLRHLDL-DSVSKLTHTPKGIRKLTCLQTLNLFVLDENKQGSKLGELNELRDLRGGL 720
           NLVNLRHL L D +    H  +G+RKL+CL+TL  FVLD  K+ +   ELN      G L
Sbjct: 661 NLVNLRHLVLPDHLDTPLHMLEGMRKLSCLRTLGYFVLD-CKRSNMQSELNVPNYFNGRL 720

Query: 721 YIKGLEQVRGNPSEARFANLKGKKGLRDLVLQWKPSSNEDESEDGLNDEIILEGLEPHPN 780
            I GLEQ+R  PS+    NLK  +GLRDL L WK S  +D+  +G  DE ++EGL+P  N
Sbjct: 721 KIVGLEQLRRTPSKVGLVNLKDIQGLRDLKLVWKLSGRDDDEGEGKGDERVMEGLQPQSN 780

Query: 781 VESVCIWRYKGEGLPNWMTNLVSKLTHISLAKCNRLQRLPQLDQLPTLKKLHLQELTCLQ 840
           VES+ I  Y G GLPNW++  +S LT I++  C+R+Q LPQL  L TLK + L+++  L+
Sbjct: 781 VESLRIEGYSGVGLPNWVSTSLSNLTKIAICNCHRVQHLPQLAHLQTLKGIFLEDMRSLK 840

Query: 841 FIDKNNPGSSKSVFFPCLDILKLKNMPSLEGWWGGVLVGDGGSTKTRRLLPKFARLTRLY 900
           F+DKN PG    + FP L  L+LKNM +LEGWW   +VG+         LP F  L  L 
Sbjct: 841 FVDKNEPG---LLCFPSLVSLRLKNMGNLEGWWESRVVGE--------TLPIFPELQYLR 900

Query: 901 IYDCPKLGWVPR--QPSLEKVELNNVGVRMVRSIGPSLYLEYVTLKNIEDLVSL 926
           I  CPKL  +P+    +L+   L +VGV++V +IGP    E V L+ I+DL  L
Sbjct: 901 IQHCPKLSLMPKIASSTLDSFFLCDVGVQVVTAIGPLWNFEVVILQEIKDLKDL 941

BLAST of Sed0001988 vs. ExPASy TrEMBL
Match: A0A1S3CEH4 (disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 2.6e-237
Identity = 485/956 (50.73%), Postives = 627/956 (65.59%), Query Frame = 0

Query: 1    MAEGVLSNFAGNVLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSK 60
            MAEG+L   AG +LT+L +   ++ GML GLKDDL +LKS VS IQ+ L DA+   T   
Sbjct: 255  MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNLPTGLD 314

Query: 61   LLKDWLNKLGEALYDADDVLDEISTQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRMAL 120
             +  WL KL   L+DA+D++DE   +   R+VMTR  HAK+VRIFFS SNQLAFNY+MA 
Sbjct: 315  SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKKVRIFFSSSNQLAFNYKMAR 374

Query: 121  KLKNIRGRLDEISEEKNKFGLIEKSEARTRYDGLSHQI--------GTHSAFIEGEVVGR 180
            ++K IR RLD I +EK+   L   S  + R   +   I         THS+  +  V+GR
Sbjct: 375  RVKRIRERLDVIDKEKSY--LWSHSFVQGRQQDVPSPIRMRMSLSRETHSSLNKERVIGR 434

Query: 181  EDDKMRIKSLLL--DETDHNNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWV 240
            +DDK  +KSLLL  D +  +NVS IAIVGMGGIGKTTLAK LYND  +SKHF++KMW+WV
Sbjct: 435  DDDKRYLKSLLLVDDMSLKDNVSLIAIVGMGGIGKTTLAKSLYNDGDVSKHFEIKMWIWV 494

Query: 241  SDEFDVKRVVEKMIESASGSKPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQ 300
            S +F  K VV+K++ESA+   P  QE+D +  +LQ +I GKKYLLVMD+I NESE++W++
Sbjct: 495  SKQFVEKIVVKKILESATKIPPIGQELDTVQSQLQDVIGGKKYLLVMDEIMNESEDEWKK 554

Query: 301  LKPLLMGCACGSKILITKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIED- 360
            LK LL+    GSKI+ITKRD KV +E+  MT  + LKGLS+  SWSLF++VAF+ ++ + 
Sbjct: 555  LKDLLVVGKRGSKIIITKRDRKVASEIEGMTTSVTLKGLSEDRSWSLFREVAFEGNLPNP 614

Query: 361  ANPKMIQLGKEILKKCGGVPLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNSMTII 420
             NPK+  +GKEI  +CGGVPL IRH+GR L S+  E EW+S  +D+L  VTQQ N +  I
Sbjct: 615  INPKLELMGKEISSRCGGVPLAIRHMGRFLCSKRCEEEWMSSLSDKLLEVTQQNNDVASI 674

Query: 421  FELSYKHLPPHLKQCFAYSSLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYF 480
             ELSY  LP HLKQCFAYSSL+ K   +  ++L++QWVA GF++S +G KSME I  +YF
Sbjct: 675  LELSYNDLPLHLKQCFAYSSLFPKVHKLKVNELIRQWVAQGFIESSKGEKSMETIGKNYF 734

Query: 481  TELCWRFFYEH-QDGNN----VCMHDMMRDLARMVASTG-YARGNLKDE--DGERIHHIS 540
             ELCWRFFYE+  D NN    V MHD+M +LAR VA    Y  G++ +     E+  H S
Sbjct: 735  DELCWRFFYENSSDENNFDDYVYMHDVMYELARKVADKKLYVCGDINNNYVVSEQTLHFS 794

Query: 541  LGHDIESWNDLPPELQKAKGLRTF---HK--SRMNHINEGVLDKLFSTFPHLRVLNMSWT 600
              + I+SW D+  +L KAKGLRTF   H      N +NE  LD+LFS+FP LRVL +  +
Sbjct: 795  FDYKIQSWQDVLSKLCKAKGLRTFMFLHSPYEEKNEVNEANLDELFSSFPRLRVLLLGGS 854

Query: 601  EFKTVPDSLNKLRHLRYLNLSDNRYLISLPNSISELVNLQTLCLNGCRELTELPRDIKNL 660
                +P+S+ KL+ LRYL+LS+N  + SLPNSI+EL +LQTL L GC  L E+PRDI  L
Sbjct: 855  NIHVLPNSIKKLKLLRYLDLSENN-MKSLPNSITELQDLQTLKLTGCYALKEMPRDINKL 914

Query: 661  VNLRHLDLDSVSKLTH-TPKGIRKLTCLQTLNLFVLDENKQGSKLGELNELRDLRGGLYI 720
            VNLRHLD    S++TH  P+G+ KL+CLQT++LFV D  K  +KL ELNEL +L G L I
Sbjct: 915  VNLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFVFDCTKT-NKLRELNELNNLTGELKI 974

Query: 721  KGLEQVRGNPSEARFANLKGKKGLRDLVLQWKPSSNEDESEDGLNDEIILEGLEPHPNVE 780
             GL+Q+R  P E    NLK KK LR L L+WK    + E E    DE +++GLEP+PNVE
Sbjct: 975  TGLQQLRSTPYEVSLVNLKDKKCLRSLKLEWKMGLYKHEDE---ADERVMKGLEPNPNVE 1034

Query: 781  SVCIWRYKGEGLPNWMTNLVSKLTHISLAKCNRLQRLPQLDQLPTLKKLHLQELTCLQFI 840
            S+ I  Y G GLP WM N   KLT I +  C+RLQ LPQ   L TLK L L++L  L+FI
Sbjct: 1035 SLGIKGYNGVGLPKWMFNCHLKLTEIEIVSCHRLQHLPQFHHLQTLKTLCLRDLRSLKFI 1094

Query: 841  DKNNPGSSKSVFFPCLDILKLKNMPSLEGWWG-GVLVGDGGSTKTRRLLPKFARLTRLYI 900
            DK +   S S+FFP L  L L NM +LEGWW     V    S   + L P F  L  L I
Sbjct: 1095 DKFDNPFSSSMFFPSLKSLCLANMANLEGWWELSRAVAGETSENIQWLPPTFPELEILDI 1154

Query: 901  YDCPKLGWVPRQPSLEK--VELNNVGVRMVRS-IGPSLY-LEYVTLKNIEDLVSLP 927
            Y CPKL  +P+ PS  K  V L +VGV++V S IGP L  L  + L+ I++L  LP
Sbjct: 1155 YQCPKLNSMPKLPSSTKAYVSLCDVGVQLVISTIGPVLLCLRTLRLEEIKNLNCLP 1203

BLAST of Sed0001988 vs. ExPASy TrEMBL
Match: A0A5A7UWP6 (Disease resistance protein RGA2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00750 PE=4 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 4.1e-235
Identity = 482/955 (50.47%), Postives = 625/955 (65.45%), Query Frame = 0

Query: 1   MAEGVLSNFAGNVLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSK 60
           MAEG+L   AG +LT+L +   ++ GML GLKDDL +LKS VS IQ+ L DA+   T   
Sbjct: 1   MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNWPTGLD 60

Query: 61  LLKDWLNKLGEALYDADDVLDEISTQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRMAL 120
            +  WL KL   L+DA+D++DE   +   R+VMTR  HAK+VRIFFS SNQLAFNY+MA 
Sbjct: 61  SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKKVRIFFSSSNQLAFNYKMAR 120

Query: 121 KLKNIRGRLDEISEEKNKFGLIEKSEARTR------YDGLSHQIGTHSAFIEGEVVGRED 180
           ++K IR RLD I +EK+        + R +         +S    THS+  +  V+GR+D
Sbjct: 121 RVKRIRERLDVIDKEKSYLWSHSFVQGRQQDVPSPIRMRMSSSRETHSSLNKERVIGRDD 180

Query: 181 DKMRIKSLLL--DETDHNNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSD 240
           DK  +KSLLL  D +  +NVS IAIVGMGGIGKTTLAK LYND  +S+HF++KMW+WVS 
Sbjct: 181 DKRYLKSLLLVDDMSLKDNVSLIAIVGMGGIGKTTLAKSLYNDGDVSEHFEIKMWIWVSK 240

Query: 241 EFDVKRVVEKMIESASGSKPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLK 300
           +F  K VV+K++ESA+   P  QE+D +  +LQ +I GKKYLLVMD+I NESE++W++LK
Sbjct: 241 QFVEKIVVKKILESATKIPPIGQELDTVQSQLQDVIGGKKYLLVMDEIMNESEDEWKKLK 300

Query: 301 PLLMGCACGSKILITKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDI-EDAN 360
            LL+    GSKI+ITKRD KV +E+  MT  + LKGLS+ SSWSLF++VAF+ ++ +  N
Sbjct: 301 DLLVVGKRGSKIIITKRDRKVASEIEGMTTSVTLKGLSEDSSWSLFREVAFEENLPKPIN 360

Query: 361 PKMIQLGKEILKKCGGVPLVIRHIGRLLYSRFSELEWISFKN-DELSRVTQQKNSMTIIF 420
           PK+  +GKEI   CGGVPL IRH+GR L S+  E EW+S  +  +   V QQ N +  I 
Sbjct: 361 PKLELMGKEISSICGGVPLAIRHMGRFLCSKRCEEEWMSSLSWKKHLEVAQQNNDVASIL 420

Query: 421 ELSYKHLPPHLKQCFAYSSLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFT 480
           ELSY  LP HLKQCFAYSSL+ K   +  ++L++QWVA GF++S +G KSME I  +YF 
Sbjct: 421 ELSYNDLPLHLKQCFAYSSLFPKGHKLKVNELIRQWVAQGFIESSKGEKSMETIGKNYFD 480

Query: 481 ELCWRFFYEH-QDGNN----VCMHDMMRDLARMVASTG-YARGNLKDE--DGERIHHISL 540
           ELCWRFFYE+  D NN    V MHD+M +LAR VA    +  G++ +     E+  H SL
Sbjct: 481 ELCWRFFYENSSDENNFDDYVYMHDVMYELARKVADKKLFVCGDINNNYVVSEQTLHFSL 540

Query: 541 GHDIESWNDLPPELQKAKGLRTF---HK--SRMNHINEGVLDKLFSTFPHLRVLNMSWTE 600
            + I+SW D+  +L KAKGLRTF   H      N +NE  LD+LFS+FP LRVL +  + 
Sbjct: 541 DYKIQSWQDVLSKLCKAKGLRTFMFLHSPYEEKNEVNEANLDELFSSFPRLRVLLLGGSN 600

Query: 601 FKTVPDSLNKLRHLRYLNLSDNRYLISLPNSISELVNLQTLCLNGCRELTELPRDIKNLV 660
              +P+S+ KL+ LRYL+LS+N  + SLPNSI+EL +LQTL L GC  L E+PRDI  LV
Sbjct: 601 IHVLPNSIKKLKLLRYLDLSENN-MKSLPNSITELQDLQTLKLTGCYALKEMPRDINKLV 660

Query: 661 NLRHLDLDSVSKLTH-TPKGIRKLTCLQTLNLFVLDENKQGSKLGELNELRDLRGGLYIK 720
           NLRHLD    S++TH  P+G+ KL+CLQT++LFV D  K  +KL ELNEL +L G L I 
Sbjct: 661 NLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFVFDCTKT-NKLRELNELNNLTGELKIT 720

Query: 721 GLEQVRGNPSEARFANLKGKKGLRDLVLQWKPSSNEDESEDGLNDEIILEGLEPHPNVES 780
           GL+Q+R  P E    NLKGKK LR L L+WK    + E E    DE +++GLEP+PNVES
Sbjct: 721 GLQQLRSTPYEVSLVNLKGKKCLRSLKLEWKMGLYKHEDE---ADERVMKGLEPNPNVES 780

Query: 781 VCIWRYKGEGLPNWMTNLVSKLTHISLAKCNRLQRLPQLDQLPTLKKLHLQELTCLQFID 840
           + I  Y G GLP WM N   KLT I +  C+RLQ LPQ   L TLK L L++L  L+FID
Sbjct: 781 LGIKGYNGVGLPKWMFNCHLKLTEIEMVSCHRLQHLPQFHHLQTLKTLCLRDLRSLKFID 840

Query: 841 KNNPGSSKSVFFPCLDILKLKNMPSLEGWWG-GVLVGDGGSTKTRRLLPKFARLTRLYIY 900
           K +   S S+FFP L  L L NM +LEGWW     V    S   + L P F  L  L IY
Sbjct: 841 KFDNPFSSSMFFPSLKSLCLANMANLEGWWELSRAVAGETSENIQWLPPTFPELEILDIY 900

Query: 901 DCPKLGWVPRQPSLEK--VELNNVGVRMVRS-IGPSLY-LEYVTLKNIEDLVSLP 927
            CPKL  +P+ PS  K  V L +VGV++V S IGP L  L  + L+ I+DL  LP
Sbjct: 901 QCPKLNSMPKLPSSTKAYVSLCDVGVQLVISTIGPVLLCLRTLRLEEIKDLNCLP 950

BLAST of Sed0001988 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 450.3 bits (1157), Expect = 4.1e-126
Identity = 298/899 (33.15%), Postives = 481/899 (53.50%), Query Frame = 0

Query: 35  LKRLKSNVSAIQAVLTDAEERSTKSKLLKDWLNKLGEALYDADDVLDEISTQALRRQVMT 94
           L+RL + +  I AVL DAEE+   + +++ W+N+L + +Y A+D LD+I+T+ALR  +  
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98

Query: 95  RGIHAKQVRIFFSKSNQLAF----NYRMALKLKNIRGRLDEISEEKNKFGLIEKSEARTR 154
               + ++R    + +   F    +  +  +L+ +  RL+ ++ ++N  GL E +    +
Sbjct: 99  ESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMIPK 158

Query: 155 YDGLSHQIGTHSAFIEGEVVGREDDKMRIKSLLLDETDHNN-VSFIAIVGMGGIGKTTLA 214
                 ++ T S   E EV GR+DDK  I   L+ E   +N ++ +AIVG+GG+GKTTL+
Sbjct: 159 -----QRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLS 218

Query: 215 KLLYNDTQLSKHFDLKMWVWVSDEFDVKRVVEKMIESASGSKPNVQEMDPLGGELQKLIE 274
           +LLYND  +  +F  K+W  VS+EFDV ++ +K+ ES +       ++D L  +L++ + 
Sbjct: 219 QLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLT 278

Query: 275 GK--KYLLVMDDIWNESEEKWEQLKPLLMGCACGSKILITKRDSKVPAEVGSMTKLINLK 334
           G    +LLV+DD+WNE+   W+ L+   +  A GS+IL+T R  +V A +     + NL+
Sbjct: 279 GTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRV-ASIMCAVHVHNLQ 338

Query: 335 GLSDASSWSLFQKVAFKVDIEDANPKMIQLGKEILKKCGGVPLVIRHIGRLLYSRFSELE 394
            LSD   WSLF K  F       N ++  L + I+ KC G+PL ++ +G +L      +E
Sbjct: 339 PLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIE 398

Query: 395 WISFKNDELSRVTQQKNSMTIIFELSYKHLPPHLKQCFAYSSLYTKNEYIDKDQLLKQWV 454
           W    +  +  +   K+++  +  +SY +LP HLK+CFAY S++ K    +KD+++  W+
Sbjct: 399 WERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWM 458

Query: 455 AHGFVQSHEGSKSMEDIANDYFTELCWRFFYEHQDGNNVCMHDMMRDLARMVASTGYARG 514
           A GF+Q    SK++E++ N+YF+EL  R   + +      MHD + +LA+  +       
Sbjct: 459 AEGFLQQTRSSKNLEELGNEYFSELESRSLLQ-KTKTRYIMHDFINELAQFAS----GEF 518

Query: 515 NLKDEDG------ERIHHISLGHDIESWNDLPPELQKAKGLRTF------HKSRMNHINE 574
           + K EDG      ER  ++S   D  +       L++ K LRTF      + SR   +++
Sbjct: 519 SSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSCCLDQ 578

Query: 575 GVLDKLFSTFPHLRVLNMSWTEF-KTVPDSLNKLRHLRYLNLSDNRYLISLPNSISELVN 634
            V +KL  T   LRVL++S  +  +  PD    + H R+L+LS    L  LP S+  + N
Sbjct: 579 MVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTE-LEKLPKSLCYMYN 638

Query: 635 LQTLCLNGCRELTELPRDIKNLVNLRHLDLDSVSKLTHTPKGIRKLTCLQTLNLFVLDEN 694
           LQTL L+ C  L ELP DI NL+NLR+LDL   +KL   P+   +L  LQTL  F +  +
Sbjct: 639 LQTLLLSYCSSLKELPTDISNLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFVSAS 698

Query: 695 KQGSKLGELNELRDLRGGLYIKGLEQVRGNPSEARFANLKGKKGLRDLVLQWKPSSNEDE 754
             GS++ EL  L DL G L I  L++V  + ++A  ANL  KK LR++   W+  S+  E
Sbjct: 699 -DGSRISELGGLHDLHGKLKIVELQRV-VDVADAAEANLNSKKHLREIDFVWRTGSSSSE 758

Query: 755 SEDG----LNDEIILEGLEPHPNVESVCIWRYKGEGLPNWMTN-LVSKLTHISLAKCNRL 814
           +        N+  + E L PH ++E + I RYKG   P+W+++   S++  I L +C   
Sbjct: 759 NNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYC 818

Query: 815 QRLPQLDQLPTLKKLHLQELTCLQFIDKNNPGSSKSVF------FPCLDILKLKNMPSLE 874
             LP L QLP LK+LH+  +  LQ I +    S + +       F  L+ L+  N+P  +
Sbjct: 819 TSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQ 878

Query: 875 GWWG-GVLVGDGGSTKTRRLLPKFARLTRLYIYDCPKL-GWVPR-QPSLEKVELNNVGV 900
            W    V  GD            F  L +L+I  CP+L G +P   PSL  + +   G+
Sbjct: 879 EWLDVRVTRGD-----------LFPSLKKLFILRCPELTGTLPTFLPSLISLHIYKCGL 911

BLAST of Sed0001988 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 414.8 bits (1065), Expect = 1.9e-115
Identity = 322/1035 (31.11%), Postives = 525/1035 (50.72%), Query Frame = 0

Query: 1   MAEGVLSNFAGNVLTQLST-EALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKS 60
           MA   LS+ A  ++ +++T + L +L         LKRLK  +     VL DA++R+   
Sbjct: 1   MANSYLSSCANVMVERINTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEHV 60

Query: 61  KLLKDWLNKLGEALYDADDVLDEISTQALRRQVMTR--GIHAKQVRIFFSKSNQLAFNYR 120
           + +K WL  + +A + A+D+LDE+ T+ALRR+V+    G+      +   +    A   +
Sbjct: 61  REVKHWLTGIKDAFFQAEDILDELQTEALRRRVVAEAGGLGGLFQNLMAGRE---AIQKK 120

Query: 121 MALKLKNIRGRLDEISEEKNKFGLIEKSE---------ARTRYDGLSHQIGTHSAFIEGE 180
           +  K++ +   L+   +     GL E SE         +R+R D L           +G 
Sbjct: 121 IEPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWRQASRSRPDDLP----------QGR 180

Query: 181 VVGREDDKMRIKSLLL--DETDHNNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKM 240
           +VGR +DK+ + +LLL  DE      + I++VGM G+GKTTL ++++ND ++++HF++KM
Sbjct: 181 LVGRVEDKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKM 240

Query: 241 WVWVSDEFDVKRVVEKMIESASGSKPNVQEMDPLGGELQKLIEGKKYLLVMDDIWNESEE 300
           W+     F+V  V + +++  + S  N +++  L  +L+K + GK++LLV+DD W+ES+ 
Sbjct: 241 WISAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKTLSGKRFLLVLDDFWSESDS 300

Query: 301 KWEQLKPLLMGCACGSKILITKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAF-KV 360
           +WE  +        GSKI++T R S++ + V    K+  +K +++   W L  + AF  +
Sbjct: 301 EWESFQVAFTDAEEGSKIVLTTR-SEIVSTVAKAEKIYQMKLMTNEECWELISRFAFGNI 360

Query: 361 DIEDANPKMIQLGKEILKKCGGVPLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNS 420
            +   N ++  +GK I ++C G+PL  R I   L S+ +  +W +   +     +   NS
Sbjct: 361 SVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKN----FSSYTNS 420

Query: 421 MTIIFELSYKHLPPHLKQCFAYSSLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIA 480
           +  + +LSY  LPP LK+CFA  S++ K    D+++L+  W+A   +     S+ +EDI 
Sbjct: 421 ILPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQPRSSRRLEDIG 480

Query: 481 NDYFTELCWRFFYEHQD--GNNVCMHDMMRDLARMVASTGYARGNLKDEDGERI----HH 540
           NDY  +L  + F++  D    +  MHD+M DLA+ V+     R  L+D++   I     H
Sbjct: 481 NDYLGDLVAQSFFQRLDITMTSFVMHDLMNDLAKAVSGDFCFR--LEDDNIPEIPSTTRH 540

Query: 541 ISLGHDIESWNDLPPELQKAKGLRTF------HKSRMNHINEGVLDKLFSTFPHLRVLNM 600
            S        +     +  A+ LRT              + E VL+ L +    LR+L++
Sbjct: 541 FSFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLESLQLTEKVLNPLLNALSGLRILSL 600

Query: 601 SWTEFKTVPDSLNKLRHLRYLNLSDNRYLISLPNSISELVNLQTLCLNGCRELTELPRDI 660
           S  +   +P SL  L+ LRYL+LS  + +  LP  +  L NLQTL L+ CR+LT LP+ I
Sbjct: 601 SHYQITNLPKSLKGLKLLRYLDLSSTK-IKELPEFVCTLCNLQTLLLSNCRDLTSLPKSI 660

Query: 661 KNLVNLRHLDLDSVSKLTHTPKGIRKLTCLQTLNLFVLDENKQGSKLGELNELRDLRGGL 720
             L+NLR LDL   + L   P GI+KL  LQ L+ FV+     G+ L EL EL  LRG L
Sbjct: 661 AELINLRLLDLVG-TPLVEMPPGIKKLRSLQKLSNFVIG-RLSGAGLHELKELSHLRGTL 720

Query: 721 YIKGLEQVRGNPSEARFANLKGKKGLRDLVLQW--KPSSNEDESEDGL--NDEIILEGLE 780
            I  L+ V    SEA+ A LK K  L  L+L+W  K S     S + L  + + +L  LE
Sbjct: 721 RISELQNV-AFASEAKDAGLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLE 780

Query: 781 PHPNVESVCIWRYKGEGLPNWM-TNLVSKLTHISLAKCNRLQRLPQLDQLPTLKKLHLQE 840
           PHP++++ CI  Y+G   P W+  +    +T ++L+ CN    LP + QLP+LK L +++
Sbjct: 781 PHPHLKTFCIESYQGGAFPKWLGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEK 840

Query: 841 LTCLQ------FIDKNNPGSSKSVFFPCLDILKLKNMPSLEGWWGGVLVGDGGSTKTRRL 900
              LQ      F  +NN   S+ V F  L ILK   MP  + W    L  DG        
Sbjct: 841 FNILQKVGLDFFFGENN---SRGVPFQSLQILKFYGMPRWDEWICPEL-EDG-------- 900

Query: 901 LPKFARLTRLYIYDCPKL--GWVPRQPSLEKVELNNVGVRMVRSIGPSLYLEYVTLKNIE 960
              F  L +L I  CP L   +    PS  +V +++  +R V S G + +      +++ 
Sbjct: 901 --IFPCLQKLIIQRCPSLRKKFPEGLPSSTEVTISDCPLRAV-SGGENSF-----RRSLT 960

Query: 961 DLVSLPKKLCSLTIRKLNISNCPSLRP------STVPPFVRVNSSNYCNDEPLLTSPDVI 990
           ++   P  + S++ R+L   + P+  P      S  P F    SS+  ND+  +TS   +
Sbjct: 961 NIPESPASIPSMSRREL---SSPTGNPKSDASTSAQPGFA---SSSQSNDDNEVTSTSSL 983

BLAST of Sed0001988 vs. TAIR 10
Match: AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 257.3 bits (656), Expect = 5.1e-68
Identity = 221/785 (28.15%), Postives = 371/785 (47.26%), Query Frame = 0

Query: 32  KDDLKRLKSNVSAIQAVLTDAEERSTKSKLLKDWLNKLGEALYDADDVL--------DEI 91
           +  L+ L+S +  +Q+ L DAE +   ++ L+  +  L E +Y+A+D+L        D+ 
Sbjct: 28  RKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELVYEAEDILVDCQLADGDDG 87

Query: 92  STQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRMALKLKNIRGRLDEI-SEEKNKFGLI 151
           + Q      ++R +H  +V + + KS           +L+ I  R+ +I S+ +  F  I
Sbjct: 88  NEQRSSNAWLSR-LHPARVPLQYKKSK----------RLQEINERITKIKSQVEPYFEFI 147

Query: 152 EKSEA--RTRYDGLSHQIGTHSAFIEGEVVGREDDKMRIKSLLLDETDHNNVSFIAIVGM 211
             S        D  S  +  H+     +VVG E DK +IK  L    D + +  +A VGM
Sbjct: 148 TPSNVGRDNGTDRWSSPVYDHT-----QVVGLEGDKRKIKEWLFRSND-SQLLIMAFVGM 207

Query: 212 GGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEFDVKRVVEKMIESASGSKPNVQEMDPL 271
           GG+GKTT+A+ ++ND ++   F+ ++WV VS  F  ++++  ++ +  G      ++  L
Sbjct: 208 GGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNL-GDASVGDDIGTL 267

Query: 272 GGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPLLMGCACGSKILITKRDSKVPAEVGSM 331
             ++Q+ + GK+YL+VMDD+W+++   W+++   L     GS I+ T+ +S         
Sbjct: 268 LRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQGLPRGQGGSVIVTTRSESVAKRVQARD 327

Query: 332 TKLINLKGLSDASSWSLFQKVAFKV-DIEDANPKMIQLGKEILKKCGGVPLVIRHIGRLL 391
            K    + LS  +SW LF  VAF   D     P++  +GKEI+ KC G+PL I+ +G LL
Sbjct: 328 DKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVGGLL 387

Query: 392 YSRFSEL-EW---ISFKNDELSRVTQQKNSMTIIFELSYKHLPPHLKQCFAYSSLYTKNE 451
             +     EW        DEL   T + +++    +LSY  LP HLK C    SLY ++ 
Sbjct: 388 LCKDHVYHEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDC 447

Query: 452 YIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCWRFFYEHQDGN------NVCMH 511
            I K QL+  W+  GFV    G +S  +   D F+ L  R   E  D           +H
Sbjct: 448 VIPKQQLVHGWIGEGFVMWRNG-RSATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIH 507

Query: 512 DMMRDLARMVASTGYARGNLKDEDGERIHHISLGHDIESWNDLPPELQKAKGLRTFHKSR 571
           DM+RDL   +A     + +  + +G    H+ +  + +    +    +    + T     
Sbjct: 508 DMVRDLVIDIAK----KDSFSNPEGLNCRHLGISGNFDE-KQIKVNHKLRGVVSTTKTGE 567

Query: 572 MNHINEGVLDKLFSTFPHLRVLNMSWTEF----KTVPDSLNKLRHLRYLNLSDNRYLISL 631
           +N +N   L K F+   +LRVL++S + F      + D +  L+HL  L+LS+   LI  
Sbjct: 568 VNKLNSD-LAKKFTDCKYLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNTHPLIQF 627

Query: 632 PNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRHLDLDSVSKLTHTPKGIRKLTCLQT 691
           P S+ +L NLQ L  + C+ L +L   I     L  LD+ +   L   PKGI  L  L+ 
Sbjct: 628 PRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEV 687

Query: 692 LNLFVLDENKQGSKLGELNELRDLRGGLYIKGLEQVRGNPSEARFANLKGKKGLRDLVLQ 751
           L  F    +  G KL E+  L +LR      GL   RG+  E        ++ L  L+  
Sbjct: 688 LLGFKPARSNNGCKLSEVKNLTNLRK----LGLSLTRGDQIE--------EEELDSLINL 747

Query: 752 WKPSSNEDESEDGLNDEII--LEGLEPHPNVESVCIWRYKGEGLPNWMT-NLVSKLTHIS 788
            K  S      D   D++I  ++ L P   +  + +  Y G+  P+W++ + +  L ++S
Sbjct: 748 SKLMSISINCYDSYGDDLITKIDALTPPHQLHELSLQFYPGKSSPSWLSPHKLPMLRYMS 775

BLAST of Sed0001988 vs. TAIR 10
Match: AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 257.3 bits (656), Expect = 5.1e-68
Identity = 221/785 (28.15%), Postives = 371/785 (47.26%), Query Frame = 0

Query: 32  KDDLKRLKSNVSAIQAVLTDAEERSTKSKLLKDWLNKLGEALYDADDVL--------DEI 91
           +  L+ L+S +  +Q+ L DAE +   ++ L+  +  L E +Y+A+D+L        D+ 
Sbjct: 28  RKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELVYEAEDILVDCQLADGDDG 87

Query: 92  STQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRMALKLKNIRGRLDEI-SEEKNKFGLI 151
           + Q      ++R +H  +V + + KS           +L+ I  R+ +I S+ +  F  I
Sbjct: 88  NEQRSSNAWLSR-LHPARVPLQYKKSK----------RLQEINERITKIKSQVEPYFEFI 147

Query: 152 EKSEA--RTRYDGLSHQIGTHSAFIEGEVVGREDDKMRIKSLLLDETDHNNVSFIAIVGM 211
             S        D  S  +  H+     +VVG E DK +IK  L    D + +  +A VGM
Sbjct: 148 TPSNVGRDNGTDRWSSPVYDHT-----QVVGLEGDKRKIKEWLFRSND-SQLLIMAFVGM 207

Query: 212 GGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEFDVKRVVEKMIESASGSKPNVQEMDPL 271
           GG+GKTT+A+ ++ND ++   F+ ++WV VS  F  ++++  ++ +  G      ++  L
Sbjct: 208 GGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNL-GDASVGDDIGTL 267

Query: 272 GGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPLLMGCACGSKILITKRDSKVPAEVGSM 331
             ++Q+ + GK+YL+VMDD+W+++   W+++   L     GS I+ T+ +S         
Sbjct: 268 LRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQGLPRGQGGSVIVTTRSESVAKRVQARD 327

Query: 332 TKLINLKGLSDASSWSLFQKVAFKV-DIEDANPKMIQLGKEILKKCGGVPLVIRHIGRLL 391
            K    + LS  +SW LF  VAF   D     P++  +GKEI+ KC G+PL I+ +G LL
Sbjct: 328 DKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVGGLL 387

Query: 392 YSRFSEL-EW---ISFKNDELSRVTQQKNSMTIIFELSYKHLPPHLKQCFAYSSLYTKNE 451
             +     EW        DEL   T + +++    +LSY  LP HLK C    SLY ++ 
Sbjct: 388 LCKDHVYHEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDC 447

Query: 452 YIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCWRFFYEHQDGN------NVCMH 511
            I K QL+  W+  GFV    G +S  +   D F+ L  R   E  D           +H
Sbjct: 448 VIPKQQLVHGWIGEGFVMWRNG-RSATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIH 507

Query: 512 DMMRDLARMVASTGYARGNLKDEDGERIHHISLGHDIESWNDLPPELQKAKGLRTFHKSR 571
           DM+RDL   +A     + +  + +G    H+ +  + +    +    +    + T     
Sbjct: 508 DMVRDLVIDIAK----KDSFSNPEGLNCRHLGISGNFDE-KQIKVNHKLRGVVSTTKTGE 567

Query: 572 MNHINEGVLDKLFSTFPHLRVLNMSWTEF----KTVPDSLNKLRHLRYLNLSDNRYLISL 631
           +N +N   L K F+   +LRVL++S + F      + D +  L+HL  L+LS+   LI  
Sbjct: 568 VNKLNSD-LAKKFTDCKYLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNTHPLIQF 627

Query: 632 PNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRHLDLDSVSKLTHTPKGIRKLTCLQT 691
           P S+ +L NLQ L  + C+ L +L   I     L  LD+ +   L   PKGI  L  L+ 
Sbjct: 628 PRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEV 687

Query: 692 LNLFVLDENKQGSKLGELNELRDLRGGLYIKGLEQVRGNPSEARFANLKGKKGLRDLVLQ 751
           L  F    +  G KL E+  L +LR      GL   RG+  E        ++ L  L+  
Sbjct: 688 LLGFKPARSNNGCKLSEVKNLTNLRK----LGLSLTRGDQIE--------EEELDSLINL 747

Query: 752 WKPSSNEDESEDGLNDEII--LEGLEPHPNVESVCIWRYKGEGLPNWMT-NLVSKLTHIS 788
            K  S      D   D++I  ++ L P   +  + +  Y G+  P+W++ + +  L ++S
Sbjct: 748 SKLMSISINCYDSYGDDLITKIDALTPPHQLHELSLQFYPGKSSPSWLSPHKLPMLRYMS 775

BLAST of Sed0001988 vs. TAIR 10
Match: AT3G46730.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 244.6 bits (623), Expect = 3.4e-64
Identity = 240/864 (27.78%), Postives = 396/864 (45.83%), Query Frame = 0

Query: 11  GNVLTQLSTEALKQLGMLLGLKDDLKRLKSNVSAIQAVLTDAEERSTKSKLLKDWLNKLG 70
           G VL ++    + ++  L+G+KDDL+ LK+ ++ I   L D E R  + ++ K+W   + 
Sbjct: 7   GFVLNKIGGYLINEVLALMGVKDDLEELKTELTCIHGYLKDVEAREREDEVSKEWTKLVL 66

Query: 71  EALYDADDVLD----EISTQALRRQVMTRGIHAKQVRIFFSKSNQLAFNYRMALKLKNIR 130
           +  YD +DVLD    ++  ++LRR ++             +K  +    Y +   ++ ++
Sbjct: 67  DIAYDIEDVLDTYFLKLEERSLRRGLLR----------LTNKIGKKRDAYNIVEDIRTLK 126

Query: 131 GRLDEISEEKNKFGLIEKSEARTR--YDGLSHQIGTHSAFIEGE-VVGREDDKMRIKSLL 190
            R+ +I+ ++  FG+   +E R     +    Q+       + E VVG EDD   +   L
Sbjct: 127 RRILDITRKRETFGIGSFNEPRGENITNVRVRQLRRAPPVDQEELVVGLEDDVKILLVKL 186

Query: 191 LDETDHNNVSFIAIVGMGGIGKTTLAKLLYNDTQLSKHFDLKMWVWVSDEFDVKRVVEKM 250
           L + + +    I+I GMGG+GKT LA+ LYN   + + FD + W +VS E+  + ++ ++
Sbjct: 187 LSDNEKDKSYIISIFGMGGLGKTALARKLYNSGDVKRRFDCRAWTYVSQEYKTRDILIRI 246

Query: 251 IES---ASGSKPN----VQEMDPLGGELQKLIEGKKYLLVMDDIWNESEEKWEQLKPLLM 310
           I S    S  +       +E + L   L  L+EGK Y++V+DD+W+   + WE LK  L 
Sbjct: 247 IRSLGIVSAEEMEKIKMFEEDEELEVYLYGLLEGKNYMVVVDDVWD--PDAWESLKRALP 306

Query: 311 GCACGSKILITKRDSKVPAEVGSMTKLINLKGLSDASSWSLFQKVAFKVDIEDANPKMIQ 370
               GSK++IT R   +   V        L+ L+   SW+LF++ AF  +IE  +  + +
Sbjct: 307 CDHRGSKVIITTRIRAIAEGVEGTVYAHKLRFLTFEESWTLFERKAFS-NIEKVDEDLQR 366

Query: 371 LGKEILKKCGGVPLVIRHIGRLLYSRFSELEWISFKNDELSRVTQQKNSMTIIFELSYKH 430
            GKE++KKCGG+PL I  +  LL SR    EW         R+      ++ +F+LS+K 
Sbjct: 367 TGKEMVKKCGGLPLAIVVLSGLL-SRKRTNEWHEVCASLWRRLKDNSIHISTVFDLSFKE 426

Query: 431 LPPHLKQCFAYSSLYTKNEYIDKDQLLKQWVAHGFVQSHEGSKSMEDIANDYFTELCWRF 490
           +   LK CF Y S++ ++  I  ++L+   VA GF+Q  E    MED+A  Y  EL  R 
Sbjct: 427 MRHELKLCFLYFSVFPEDYEIKVEKLIHLLVAEGFIQEDE-EMMMEDVARCYIDELVDRS 486

Query: 491 F-----YEHQDGNNVCMHDMMRDLA-RMVASTGYAR-GNLKDEDGERIHHISLGHDIESW 550
                  E     +  +HD++RDLA +      +    N K    +      + H +  +
Sbjct: 487 LVKAERIERGKVMSCRIHDLLRDLAIKKAKELNFVNVYNEKQHSSDICRREVVHHLMNDY 546

Query: 551 NDLPPELQKAKGLRTFHKSRMNHINEGVLDKLFSTFPHLRVLNMSWTEF------KTVPD 610
                 + K      F   R      G ++        LRVLNM    F       T+PD
Sbjct: 547 YLCDRRVNKRMRSFLFIGERRGF---GYVNTTNLKLKLLRVLNMEGLLFVSKNISNTLPD 606

Query: 611 SLNKLRHLRYLNLSDNRYLISLPNSISELVNLQTLCLNGCRELTELPRDIKNLVNLRHLD 670
            + +L HLRYL ++D  Y+  LP SIS L  LQTL  +G  +  +   D+  L +LRH+ 
Sbjct: 607 VIGELIHLRYLGIADT-YVSILPASISNLRFLQTLDASG-NDPFQYTTDLSKLTSLRHVI 666

Query: 671 LDSVSKLTHTPKGIRKLTCLQTLNLFVLDENKQGSKLGE--LNELRDLRGGLYIKGLEQV 730
              V +       I +   LQTL       +   SKL    L  L+DL    + K ++Q 
Sbjct: 667 GKFVGECL-----IGEGVNLQTLRSI---SSYSWSKLNHELLRNLQDLEIYDHSKWVDQR 726

Query: 731 RGNPSEARFANLKGKKGLRDLVLQWK--PSSNEDESEDGLND-----------------E 790
           R       F +    K LR L L+ +    S+E  +  GL D                 E
Sbjct: 727 R---VPLNFVSFSKPKNLRVLKLEMRNFKLSSESRTTIGLVDVNFPSLESLTLVGTTLEE 786

Query: 791 IILEGLEPHPNVESVCI--WRYKGEGLPNWMTNLVSKLTHISLAKCNRLQRLPQL----D 818
             +  L+  P +E + +    Y G  + +       +L ++ ++   R   L +L    +
Sbjct: 787 NSMPALQKLPRLEDLVLKDCNYSGVKIMSISAQGFGRLKNLEMSMERRGHGLDELRIEEE 839