Sed0001282 (gene) Chayote v1

Overview
NameSed0001282
Typegene
OrganismSechium edule (Chayote v1)
DescriptionRING-type E3 ubiquitin transferase
LocationLG04: 41224796 .. 41226763 (-)
RNA-Seq ExpressionSed0001282
SyntenySed0001282
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTAAATCTCTTCTTATAGTTTTTTCTTTTGAACTCTTACTTTATGATGATTTACTCTTGGAGGCAGTGGAGGGCCACCCGGCGAAGAATGACGAGGGATGGCGGTGTCGGCATCGGGGAGCTGACGATCCCGGCCAATTTCCAGTGCCCGATATCGCTTGACTTGATGAAAGATCCAGTTATCTTGTCGACCGGGATAACGTACGATCGAACCAGCATCGAGACTTGGATTGAAGCAGGGAATTTTAGGTGCCCCATCACCAAACAGCCATTGCAGAGCCTTGATTCTATTCCTAATCATAACATTAGGAGGATGATCCAAGATTGGTGTGTTGACAACAGGGCTTATGGAATTGAGAGGATTCCAACACCAAGGGTTCCCGTAAGTCCCATGCAGATTCGAGAGATTCTCGAGAAGCTTTCGAATGCTGCACGGCGGGAAGATGGTGATAGATGCAAGTCCCTGGTGGAGAAGATCAAGATGCTGGGAAAAGAAAGTGAAAGGAACAAAAAATGCATTATCACTAATGGAACAGGTTTTTAAACCAACATGTTACTCAAAACTTTAGCGTTTGATTTTTTGTTTTATTTTGTTTTTCACATTGCACTATAATTCTCTATATGGGGTTTTATATATCTTTTGAAAAAGTTTGAATTTTGAGTTTAATTTGAAAAACAAAAACAATTTTTTAAGCACGCATACGAATGGAAATATAACCCAAAAAAAAAAAAATCTGTAGACATTAAGTAGTATTCATAGGTTTAGTTTTTAAAAATAAATGGTTATCAATTGGAGCACACTTTAAGGTACTTTTATGAACTCAAAACTTCAACAATATCTAAATCTTTTATTCATATGAAAAATGCAGCTATTGTATTATCGTCCGCGTTCGAAGCTTTTTCAAAACCCGAAACATTCGAGAAAAGTGTCGAGGTTTTAGAAGAAATATTATCAGCCATGGCAATCATCTTTCCTCTACAAGAAGAAGCCATCAAAAACCTAAAATCAGAAACTTCATTGCAATCATTGGTTTGGTTTTTGAGAGGAGGAGACTTATCAGGAAGAAGAAATTCAATTTTGGTATTGAAAGAAATCATTTCATCATATCCAGAAAAAGTAACAAAATTAGCTGAAATCCAAGGAGTTTTAGAGAGTCTGGTAAAGCTCATAAAAGAGCCCATTTGCTCATCTTCAAAAAAAGCCTCACTTTTCATAACATATCACATCATAGCCTCCTCTTCCAATCCTAATGGATTCACAAAATCACTTCTACAAATGGGGTTCCTCCCTTTGCTTCTTGAAATGCTTGTCGACTCCGAGAGGAGCGTGTGCGAGAGGGCATTGGGAGCGTTTGATGGAATTTGTGAAAGCGAGGAGGGAAGAGAAGAGGCATATGCGCACGCTTTGACGATACCCGTGATAGTGAAAAAGATACTTCGAGTTTCGGATTTGGCGACCGAGCTAGCGGTCTCCATTGTTTGGAAGCTTGTAAAGCATGAAGAAGATGATGGATGGGTTAAAGTGGATGCACTTCAAGTGGGAGCATTTCAAAAGCTTTTGTTGTTGTTGCAAGTTGGTTGCAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAAATGTTGAATAGTGTTCATAGGAATAGGTTAGAGTGTATTGATTCATTGGATTTTAAGAATCTTAAAAGACCCTTTTGAATTGAGAGAAAAATATTTTATTCATAAGAATTAATTTGTAAAATTGTAAGTTGAGTAATAAGTTGTGTGTGGATAAGGATTTTTGTGATTTGTGTTTTAATCTTTTATTTTTATGCAATTTTATTATTGTACAAAGAGTCAACACGAGTACAGGTATGCCATTTTTATGTGATTCTTCGTGATGAACAAATCTATAGCATTAATTTTGATGTGTGATGTATTGTTTTTAATTCAATTCACCAAGAGAAATCAAGATAGATTATCTTTTT

mRNA sequence

TCTAAATCTCTTCTTATAGTTTTTTCTTTTGAACTCTTACTTTATGATGATTTACTCTTGGAGGCAGTGGAGGGCCACCCGGCGAAGAATGACGAGGGATGGCGGTGTCGGCATCGGGGAGCTGACGATCCCGGCCAATTTCCAGTGCCCGATATCGCTTGACTTGATGAAAGATCCAGTTATCTTGTCGACCGGGATAACGTACGATCGAACCAGCATCGAGACTTGGATTGAAGCAGGGAATTTTAGGTGCCCCATCACCAAACAGCCATTGCAGAGCCTTGATTCTATTCCTAATCATAACATTAGGAGGATGATCCAAGATTGGTGTGTTGACAACAGGGCTTATGGAATTGAGAGGATTCCAACACCAAGGGTTCCCGTAAGTCCCATGCAGATTCGAGAGATTCTCGAGAAGCTTTCGAATGCTGCACGGCGGGAAGATGGTGATAGATGCAAGTCCCTGGTGGAGAAGATCAAGATGCTGGGAAAAGAAAGTGAAAGGAACAAAAAATGCATTATCACTAATGGAACAGCTATTGTATTATCGTCCGCGTTCGAAGCTTTTTCAAAACCCGAAACATTCGAGAAAAGTGTCGAGGTTTTAGAAGAAATATTATCAGCCATGGCAATCATCTTTCCTCTACAAGAAGAAGCCATCAAAAACCTAAAATCAGAAACTTCATTGCAATCATTGGTTTGGTTTTTGAGAGGAGGAGACTTATCAGGAAGAAGAAATTCAATTTTGGTATTGAAAGAAATCATTTCATCATATCCAGAAAAAGTAACAAAATTAGCTGAAATCCAAGGAGTTTTAGAGAGTCTGGTAAAGCTCATAAAAGAGCCCATTTGCTCATCTTCAAAAAAAGCCTCACTTTTCATAACATATCACATCATAGCCTCCTCTTCCAATCCTAATGGATTCACAAAATCACTTCTACAAATGGGGTTCCTCCCTTTGCTTCTTGAAATGCTTGTCGACTCCGAGAGGAGCGTGTGCGAGAGGGCATTGGGAGCGTTTGATGGAATTTGTGAAAGCGAGGAGGGAAGAGAAGAGGCATATGCGCACGCTTTGACGATACCCGTGATAGTGAAAAAGATACTTCGAGTTTCGGATTTGGCGACCGAGCTAGCGGTCTCCATTGTTTGGAAGCTTGTAAAGCATGAAGAAGATGATGGATGGGTTAAAGTGGATGCACTTCAAGTGGGAGCATTTCAAAAGCTTTTGTTGTTGTTGCAAGTTGGTTGCAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAAATGTTGAATAGTGTTCATAGGAATAGGTTAGAGTGTATTGATTCATTGGATTTTAAGAATCTTAAAAGACCCTTTTGAATTGAGAGAAAAATATTTTATTCATAAGAATTAATTTGTAAAATTGTAAGTTGAGTAATAAGTTGTGTGTGGATAAGGATTTTTGTGATTTGTGTTTTAATCTTTTATTTTTATGCAATTTTATTATTGTACAAAGAGTCAACACGAGTACAGGTATGCCATTTTTATGTGATTCTTCGTGATGAACAAATCTATAGCATTAATTTTGATGTGTGATGTATTGTTTTTAATTCAATTCACCAAGAGAAATCAAGATAGATTATCTTTTT

Coding sequence (CDS)

ATGATGATTTACTCTTGGAGGCAGTGGAGGGCCACCCGGCGAAGAATGACGAGGGATGGCGGTGTCGGCATCGGGGAGCTGACGATCCCGGCCAATTTCCAGTGCCCGATATCGCTTGACTTGATGAAAGATCCAGTTATCTTGTCGACCGGGATAACGTACGATCGAACCAGCATCGAGACTTGGATTGAAGCAGGGAATTTTAGGTGCCCCATCACCAAACAGCCATTGCAGAGCCTTGATTCTATTCCTAATCATAACATTAGGAGGATGATCCAAGATTGGTGTGTTGACAACAGGGCTTATGGAATTGAGAGGATTCCAACACCAAGGGTTCCCGTAAGTCCCATGCAGATTCGAGAGATTCTCGAGAAGCTTTCGAATGCTGCACGGCGGGAAGATGGTGATAGATGCAAGTCCCTGGTGGAGAAGATCAAGATGCTGGGAAAAGAAAGTGAAAGGAACAAAAAATGCATTATCACTAATGGAACAGCTATTGTATTATCGTCCGCGTTCGAAGCTTTTTCAAAACCCGAAACATTCGAGAAAAGTGTCGAGGTTTTAGAAGAAATATTATCAGCCATGGCAATCATCTTTCCTCTACAAGAAGAAGCCATCAAAAACCTAAAATCAGAAACTTCATTGCAATCATTGGTTTGGTTTTTGAGAGGAGGAGACTTATCAGGAAGAAGAAATTCAATTTTGGTATTGAAAGAAATCATTTCATCATATCCAGAAAAAGTAACAAAATTAGCTGAAATCCAAGGAGTTTTAGAGAGTCTGGTAAAGCTCATAAAAGAGCCCATTTGCTCATCTTCAAAAAAAGCCTCACTTTTCATAACATATCACATCATAGCCTCCTCTTCCAATCCTAATGGATTCACAAAATCACTTCTACAAATGGGGTTCCTCCCTTTGCTTCTTGAAATGCTTGTCGACTCCGAGAGGAGCGTGTGCGAGAGGGCATTGGGAGCGTTTGATGGAATTTGTGAAAGCGAGGAGGGAAGAGAAGAGGCATATGCGCACGCTTTGACGATACCCGTGATAGTGAAAAAGATACTTCGAGTTTCGGATTTGGCGACCGAGCTAGCGGTCTCCATTGTTTGGAAGCTTGTAAAGCATGAAGAAGATGATGGATGGGTTAAAGTGGATGCACTTCAAGTGGGAGCATTTCAAAAGCTTTTGTTGTTGTTGCAAGTTGGTTGCAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAAATGTTGAATAGTGTTCATAGGAATAGGTTAGAGTGTATTGATTCATTGGATTTTAAGAATCTTAAAAGACCCTTTTGA

Protein sequence

MMIYSWRQWRATRRRMTRDGGVGIGELTIPANFQCPISLDLMKDPVILSTGITYDRTSIETWIEAGNFRCPITKQPLQSLDSIPNHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIREILEKLSNAARREDGDRCKSLVEKIKMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETFEKSVEVLEEILSAMAIIFPLQEEAIKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEIISSYPEKVTKLAEIQGVLESLVKLIKEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQMGFLPLLLEMLVDSERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKILRVSDLATELAVSIVWKLVKHEEDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKATELLKMLNSVHRNRLECIDSLDFKNLKRPF
Homology
BLAST of Sed0001282 vs. NCBI nr
Match: KAG6599923.1 (U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 695.3 bits (1793), Expect = 3.4e-196
Identity = 352/443 (79.46%), Postives = 398/443 (89.84%), Query Frame = 0

Query: 2   MIYSWRQWRATRRRMTRDGGVGIGELTIPANFQCPISLDLMKDPVILSTGITYDRTSIET 61
           MI SWR+ RAT RR  R  G  + ELTIP++F+CPISLD+MKDPV LSTGITYDR SIET
Sbjct: 1   MISSWRRRRAT-RRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIET 60

Query: 62  WIEAGNFRCPITKQPLQSLDSIPNHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIRE 121
           WIE GNF CPIT QPLQ+LDSIPNHNIR+MIQDWCV+N+AYG+ERIPTPRVPVSP+QIR+
Sbjct: 61  WIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRD 120

Query: 122 ILEKLSNAARREDGDRCKSLVEKIKMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETF 181
           ILEK++ AARR DGD CKS VEKIKML KESERNKKCI+ NG + V+SSAF+A SK E  
Sbjct: 121 ILEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPL 180

Query: 182 EKSVEVLEEILSAMAIIFPLQEEAIKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEII 241
           EKS+E+LEE++SAMAI+FPLQEE+IKNLKS+ SLQ+LV F++GGDLS RRNSIL+LK+II
Sbjct: 181 EKSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGDLSRRRNSILILKQII 240

Query: 242 SSYPEKVTKLAEIQGVLESLVKLIKEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQM 301
           SSYP+K TKL EIQG LE LVKLIKEPICSSSKKASLFITYHIIASSSNP  FTK+LL+M
Sbjct: 241 SSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEM 300

Query: 302 GFLPLLLEMLVDSERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKILRVSDLAT 361
           GF+ LLLE LVD+ERSVCERALGAFDGICES+EGREEAYAHALT+PVIVKKILRVSDLAT
Sbjct: 301 GFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLAT 360

Query: 362 ELAVSIVWKLVKH----EEDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKATELLKML 421
           EL+VSIVWKLVKH    EE+DG VKV+ALQVGAFQKLLLLLQVGCSEWTKEKATELLK+L
Sbjct: 361 ELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLL 420

Query: 422 NSVHRNRLECIDSLDFKNLKRPF 441
           N++HR+ LECIDSLDFK +KRPF
Sbjct: 421 NNLHRDSLECIDSLDFKGIKRPF 442

BLAST of Sed0001282 vs. NCBI nr
Match: XP_022942705.1 (U-box domain-containing protein 21-like [Cucurbita moschata])

HSP 1 Score: 694.5 bits (1791), Expect = 5.9e-196
Identity = 352/441 (79.82%), Postives = 395/441 (89.57%), Query Frame = 0

Query: 2   MIYSWRQWRATRRRMTRDGGVGIGELTIPANFQCPISLDLMKDPVILSTGITYDRTSIET 61
           MI SWRQ RAT RR  R  G  + ELTIP++F+CPISLD+MKDPV LSTGITYDR SIET
Sbjct: 1   MISSWRQRRAT-RRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIET 60

Query: 62  WIEAGNFRCPITKQPLQSLDSIPNHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIRE 121
           WIE GNF CPIT QPLQ+LDSIPNHNIR+MIQDWCV+N+AYG+ERIPTPRVPVSP+QIR+
Sbjct: 61  WIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRD 120

Query: 122 ILEKLSNAARREDGDRCKSLVEKIKMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETF 181
           ILEK++ AARR DGD CKS VEKIKML KESERNKKCI+ NG + V+SSAF+A SK E  
Sbjct: 121 ILEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPL 180

Query: 182 EKSVEVLEEILSAMAIIFPLQEEAIKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEII 241
           EKS+E+LEE++SAMAI+FPLQEE+IKNLKSE SLQ+LV F++GGDLS RRNSIL+LK+II
Sbjct: 181 EKSIEILEEMISAMAIMFPLQEESIKNLKSEASLQALVLFMKGGDLSRRRNSILILKQII 240

Query: 242 SSYPEKVTKLAEIQGVLESLVKLIKEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQM 301
           SSYP+K TKL EIQG LE LVKLIKEPICSSSKKASLFITYHIIASSSNP  FTK LL+M
Sbjct: 241 SSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKVLLEM 300

Query: 302 GFLPLLLEMLVDSERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKILRVSDLAT 361
           GF+ LLLE LVD+ERSVCERALGAFDGICE +EGREEAYAHALT+PVIVKKILRVSDLAT
Sbjct: 301 GFVSLLLETLVDAERSVCERALGAFDGICERKEGREEAYAHALTMPVIVKKILRVSDLAT 360

Query: 362 ELAVSIVWKLVKH--EEDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKATELLKMLNS 421
           EL+VSIVWKLVKH  EE+DG  KV+ALQVGAFQKLLLLLQVGCSEWTKEKATELLK+LN+
Sbjct: 361 ELSVSIVWKLVKHESEEEDGRAKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNN 420

Query: 422 VHRNRLECIDSLDFKNLKRPF 441
           +HR+ LECIDSLDFK +KRPF
Sbjct: 421 LHRDSLECIDSLDFKGIKRPF 440

BLAST of Sed0001282 vs. NCBI nr
Match: XP_022995728.1 (U-box domain-containing protein 21-like [Cucurbita maxima])

HSP 1 Score: 694.5 bits (1791), Expect = 5.9e-196
Identity = 350/441 (79.37%), Postives = 395/441 (89.57%), Query Frame = 0

Query: 2   MIYSWRQWRATRRRMTRDGGVGIGELTIPANFQCPISLDLMKDPVILSTGITYDRTSIET 61
           MI SWR+ RATRR     GG  + ELTIP++F+CPISLD+MKDPV LSTGITYDR SIET
Sbjct: 1   MISSWRRRRATRRTARGTGGC-MRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIET 60

Query: 62  WIEAGNFRCPITKQPLQSLDSIPNHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIRE 121
           WIE GNF CPIT QPLQ+LDSIPNHNIR+MIQDWCV+N+AYG+ERIPTPRVP SP+QIR+
Sbjct: 61  WIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPASPVQIRD 120

Query: 122 ILEKLSNAARREDGDRCKSLVEKIKMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETF 181
           IL+K++ A RR DGD CKS VEKIKML KESERNKKCI+ NG + V+SSAF+A SK E  
Sbjct: 121 ILDKITAATRRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPL 180

Query: 182 EKSVEVLEEILSAMAIIFPLQEEAIKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEII 241
           EKS+E+LEE++S MAI+FPLQEE+IKNLKSE SLQ+L  F+RGGDLS RRNSIL+LKEII
Sbjct: 181 EKSIEILEEMISGMAIMFPLQEESIKNLKSEASLQALALFMRGGDLSRRRNSILILKEII 240

Query: 242 SSYPEKVTKLAEIQGVLESLVKLIKEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQM 301
           SSYP+K TKL EIQG LE LVKLIKEPICSSSKKASLFITYHIIASSSNP  FTK+LLQM
Sbjct: 241 SSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLQM 300

Query: 302 GFLPLLLEMLVDSERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKILRVSDLAT 361
           GF+ LLLE LVD+ERS+CERALG FDGICES+EGREEAYAHALT+PVIVKKILRVSDLAT
Sbjct: 301 GFVSLLLETLVDAERSICERALGVFDGICESKEGREEAYAHALTMPVIVKKILRVSDLAT 360

Query: 362 ELAVSIVWKLVKH--EEDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKATELLKMLNS 421
           EL+VSIVWKLVKH  EE+DG VKV+ALQVGAFQKLLLLLQVGCSEWTKEKATELLK+LN+
Sbjct: 361 ELSVSIVWKLVKHESEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNN 420

Query: 422 VHRNRLECIDSLDFKNLKRPF 441
           +HR+RLECIDSLDFK++KRPF
Sbjct: 421 LHRDRLECIDSLDFKDIKRPF 440

BLAST of Sed0001282 vs. NCBI nr
Match: KAG7030606.1 (U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 694.1 bits (1790), Expect = 7.7e-196
Identity = 352/443 (79.46%), Postives = 398/443 (89.84%), Query Frame = 0

Query: 2   MIYSWRQWRATRRRMTRDGGVGIGELTIPANFQCPISLDLMKDPVILSTGITYDRTSIET 61
           MI SWR+ RAT RR  R  G  + ELTIP++F+CPISLD+MKDPV LSTGITYDR SIET
Sbjct: 1   MISSWRRRRAT-RRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIET 60

Query: 62  WIEAGNFRCPITKQPLQSLDSIPNHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIRE 121
           WIE GNF CPIT QPLQ+LDSIPNHNIR+MIQDWCV+N+AYG+ERIPTPRVPVSP+QIR+
Sbjct: 61  WIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRD 120

Query: 122 ILEKLSNAARREDGDRCKSLVEKIKMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETF 181
           ILEK++ AARR DGD CKS VEKIKML KESERNKKCI+ NG + V+SSAF+AFSK E  
Sbjct: 121 ILEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQAFSKQEPL 180

Query: 182 EKSVEVLEEILSAMAIIFPLQEEAIKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEII 241
           EKS+E+LEE++SAMAI+FPLQEE+IKNLKS+ SLQ+LV F++GG LS RRNSIL+LK+II
Sbjct: 181 EKSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGVLSRRRNSILILKQII 240

Query: 242 SSYPEKVTKLAEIQGVLESLVKLIKEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQM 301
           SSYP+K TKL EIQG LE LVKLIKEPICSSSKKASLFITYHIIASSSNP  FTK+LL+M
Sbjct: 241 SSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEM 300

Query: 302 GFLPLLLEMLVDSERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKILRVSDLAT 361
           GF+ LLLE LVD+ERSVCERALGAFDGICES+EGREEAYAHALT+PVIVKKILRVSDLAT
Sbjct: 301 GFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLAT 360

Query: 362 ELAVSIVWKLVKH----EEDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKATELLKML 421
           EL+VSIVWKLVKH    EE+DG VKV+ALQVGAFQKLLLLLQVGCSEWTKEKATELLK+L
Sbjct: 361 ELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLL 420

Query: 422 NSVHRNRLECIDSLDFKNLKRPF 441
           N++HR+ LECIDSLDFK +KRPF
Sbjct: 421 NNLHRDSLECIDSLDFKGIKRPF 442

BLAST of Sed0001282 vs. NCBI nr
Match: XP_023512745.1 (U-box domain-containing protein 21-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 693.7 bits (1789), Expect = 1.0e-195
Identity = 352/443 (79.46%), Postives = 397/443 (89.62%), Query Frame = 0

Query: 2   MIYSWRQWRATRRRMTRDGGVGIGELTIPANFQCPISLDLMKDPVILSTGITYDRTSIET 61
           MI SWR+ RAT RR  R  G  + ELTIP++F+CPISLD+MKDPV LSTGITYDR SIET
Sbjct: 1   MISSWRRRRAT-RRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIET 60

Query: 62  WIEAGNFRCPITKQPLQSLDSIPNHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIRE 121
           WIE GNF CPIT QPLQ+LDSIPNHNIR+MIQDWCV+N+ YG+ERIPTPRVPVSP+QI +
Sbjct: 61  WIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKDYGVERIPTPRVPVSPVQIGD 120

Query: 122 ILEKLSNAARREDGDRCKSLVEKIKMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETF 181
           ILEK++ AARR DGD CKS VEKIKML KESERNKKCI+ NG + V+SSAF+A SK E  
Sbjct: 121 ILEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPL 180

Query: 182 EKSVEVLEEILSAMAIIFPLQEEAIKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEII 241
           EKS+E+LEE++SAMAI+FPLQEE+IKNLKS+ SLQ+LV F++GGDLS RRNSIL+LKEII
Sbjct: 181 EKSIEILEEMISAMAIMFPLQEESIKNLKSKASLQALVLFMKGGDLSRRRNSILILKEII 240

Query: 242 SSYPEKVTKLAEIQGVLESLVKLIKEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQM 301
           SSYP+K TKL EIQG LE LVKLIKEPICSSSKKASLFITYHIIASSSNP  FTK+LL+M
Sbjct: 241 SSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLEM 300

Query: 302 GFLPLLLEMLVDSERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKILRVSDLAT 361
           GF+ LLLE LVD+ERSVCERALGAFDGICES+EGREEAYAHALT+PVIVKKILRVSDLAT
Sbjct: 301 GFVSLLLETLVDAERSVCERALGAFDGICESKEGREEAYAHALTMPVIVKKILRVSDLAT 360

Query: 362 ELAVSIVWKLVKH----EEDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKATELLKML 421
           EL+VSIVWKLVKH    EE+DG VKV+ALQVGAFQKLLLLLQVGCSEWTKEKATELLK+L
Sbjct: 361 ELSVSIVWKLVKHESEEEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLL 420

Query: 422 NSVHRNRLECIDSLDFKNLKRPF 441
           N++HR+RLECIDSLDFK +KRPF
Sbjct: 421 NNLHRDRLECIDSLDFKGIKRPF 442

BLAST of Sed0001282 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.1e-103
Identity = 203/416 (48.80%), Postives = 278/416 (66.83%), Query Frame = 0

Query: 26  ELTIPANFQCPISLDLMKDPVILSTGITYDRTSIETWIEAGNFRCPITKQPLQSLDSIPN 85
           E+TIP  FQCPIS+DLMKDPVI+STGITYDR SIETWI +GN  CP+T   L + D IPN
Sbjct: 28  EITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPN 87

Query: 86  HNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIREILEKLSNAARREDGDRCKSLVEKI 145
           H IR+MIQ WCV+  +  I+RIPTPRVP+ P ++ EI  KLS+A RR D ++C  ++EKI
Sbjct: 88  HTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKI 147

Query: 146 KMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETFEKSVEVLEEILSAMAIIFPLQEEA 205
           K LG ESE+N+KC+  N    VL   F+ FS     EK   +L EILS +  +FP+  E 
Sbjct: 148 KKLGDESEKNRKCVNENSVGWVLCDCFDKFSGD---EKLTFMLNEILSLLTWMFPIGLEG 207

Query: 206 IKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEIISSYPEKVTKLAEIQGVLESLVKLI 265
           I  L S TS + +   L+  D S R+N+  ++KEI+S    +V   A   GV E+LVKLI
Sbjct: 208 ISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKLI 267

Query: 266 KEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQMGFLPLLLEMLVDSERSVCERALGA 325
           ++ + SSS K+SL   Y ++             L++G + + +EM+VD+E SVCE+AL  
Sbjct: 268 RDSVSSSSTKSSLIAIYQMVLQKPE---IASEFLEIGLVSITVEMIVDAENSVCEKALAV 327

Query: 326 FDGICESEEGREEAYAHALTIPVIVKKILRVSDLATELAVSIVWKLVKHEEDDGWVKVDA 385
            D ICE+E GREE   +AL +P++VKKI +VS+LAT  ++S++ KL K    +     DA
Sbjct: 328 LDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWK--TGNTVAVEDA 387

Query: 386 LQVGAFQKLLLLLQVGCSEWTKEKATELLKMLNSVHRNRLECIDSL-DFKNLKRPF 441
           +++GAFQK+LL+LQVG  E TKEKATELLKM+N+  +   +C+DSL +FK +K+PF
Sbjct: 388 VRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYIKKPF 435

BLAST of Sed0001282 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 6.5e-89
Identity = 189/419 (45.11%), Postives = 265/419 (63.25%), Query Frame = 0

Query: 26  ELTIPANFQCPISLDLMKDPVILSTGITYDRTSIETWIEAGNFRCPITKQPLQSLDSIPN 85
           E+TIP+ FQCPIS +LMKDPVI+++GITYDR +IE W E+G   CP+T   L SL+ IPN
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLEQIPN 89

Query: 86  HNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIREILEKLSNAARREDGDRCKSLVEKI 145
           H IRRMIQ WC  +   GIERIPTPRVPV+  Q+ EI E+LS A RR D   C  +V K+
Sbjct: 90  HTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMVTKM 149

Query: 146 KMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETFEKSVEVLEEILSAMAIIFPLQEEA 205
             LGKESERN+KC+  NG  +VL   F+AFS  E    S+ +LEE +S +  + P+  E 
Sbjct: 150 TRLGKESERNRKCVKENGAGLVLCVCFDAFS--ENANASL-LLEETVSVLTWMLPIGLEG 209

Query: 206 IKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEIISSYPEKVTKLAEIQGVLESLVKLI 265
              L + +S   LV  LR GD    +N+  ++KE++      V  L +I GV E+ +K I
Sbjct: 210 QSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAFMKSI 269

Query: 266 KEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQMGFLPLLLEMLVDSERSVCERALGA 325
                 S+   SL   +H+I ++          L++  + + +EMLVDSE SVCE+AL  
Sbjct: 270 NR---DSTCVNSLISIHHMILTNQET---VSRFLELDLVNITVEMLVDSENSVCEKALTV 329

Query: 326 FDGICESEEGREEAYAHALTIPVIVKKILRVSDLATELAVSIVWKLVKHEEDDGWVKVDA 385
            + ICE++EGRE+   + L IP++VKKIL++S+   +  VS++WK+ K    DG    +A
Sbjct: 330 LNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK--SGDGSEVEEA 389

Query: 386 LQVGAFQKLLLLLQVGCSEWTKEKATELLKMLNSVHRNR----LECIDSLDFKNLKRPF 441
           L++GAF+KL+++LQVGC E TKEK TELLKM+N V +           S++FK++K+PF
Sbjct: 390 LRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEFKHVKKPF 431

BLAST of Sed0001282 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 6.8e-54
Identity = 134/403 (33.25%), Postives = 223/403 (55.33%), Query Frame = 0

Query: 20  GGVGIGELTIPANFQCPISLDLMKDPVILSTGITYDRTSIETWIEAGNFR-CPITKQPLQ 79
           GG+   E+ IP  F CPISL++MKDPVI+STGITYDR SIE W+ AG    CP+TKQ + 
Sbjct: 3   GGIMDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDIT 62

Query: 80  SLDSIPNHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIREILEKLSNAARREDGDRC 139
             D  PNH +RR+IQ WC  N +YG+ERIPTPR P+   +I +++    +A+  E+  +C
Sbjct: 63  DADLTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIR--DSASSHENQVKC 122

Query: 140 KSLVEKIKMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETF-EKSVEVLEEILSAMAI 199
              +++++ +  E+  NK+C+   G    L++     S+  +  ++++ +L  + ++  +
Sbjct: 123 ---LKRLRQIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETV 182

Query: 200 IFPLQEEAIKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEIISSYPEKVTKLAEIQGV 259
           +    +  + N K    ++SL   ++ G    R  + L+LK I+    + +  +     V
Sbjct: 183 L----KNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNIL-EVADPMQSMTLKPEV 242

Query: 260 LESLVKLIKEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQMGFLPLLLEMLVD---- 319
              +V+++ + I   + KA++ I  +I     N +      ++ G + +++E+L+D    
Sbjct: 243 FTEVVQILDDRISQKATKAAMHILVNICPWGRNRH----KAVEAGVISVIIELLMDESFT 302

Query: 320 SERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKILRVSDLATELAVSIVWKLVK 379
           SER   E A+   D +C+  EGR E   H   I V+ KKILRVS  A++ AV ++  + +
Sbjct: 303 SERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGR 362

Query: 380 HEEDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKATELLKM 417
                  +  + LQ+G   KL L+LQV C   TKEKA ELLK+
Sbjct: 363 FCATPALLH-EMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKL 390

BLAST of Sed0001282 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.1e-51
Identity = 133/395 (33.67%), Postives = 207/395 (52.41%), Query Frame = 0

Query: 27  LTIPANFQCPISLDLMKDPVILSTGITYDRTSIETWIEAGNFRCPITKQPLQSLDSIPNH 86
           + IP +F+CPISLDLM DPV +STG TYDRTSI++WI  GN  CP+T+  L     IPNH
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNH 71

Query: 87  NIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIREILEKLSNAARREDGDRCK-SLVEKI 146
            +RR+IQ+WCV NR+ G+ERIPTP+ P  P+ +R +L + S         R + + + ++
Sbjct: 72  TLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRL 131

Query: 147 KMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETFEKSVEVLEEILSAMAIIFPLQEEA 206
           + L ++SE+N+  I  +    +L      F+  ET   S E++ E L+ + ++   + E 
Sbjct: 132 RGLARDSEKNRVLIAGHNAREILVRIL--FADIETTSLSSELVSESLALLVLLHMTETEC 191

Query: 207 IKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEIISSYPEKVTKL--AEIQGVLESLVK 266
                  + +  +   L    +  R N+  +++ +++       KL  +    + E ++ 
Sbjct: 192 EAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEGVLD 251

Query: 267 LIKEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQMGFLPLLLEML-VDSERSVCERA 326
           L+K PI   S + +L I    I +           +  G   +L++ L  D +R   ER 
Sbjct: 252 LLKNPI---SSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDTERG 311

Query: 327 LGAFDGICESEEGREEAYAHALTIPVIVKKILRVSDLATELAVSIVWKLVKHEEDDGWVK 386
           L   + +C   EG      HALT+P++VK ILRVSD ATE A   +  L   EE     +
Sbjct: 312 LATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTAEER---CR 371

Query: 387 VDALQVGAFQKLLLLLQVGCSEWTKEKATELLKML 418
            +A   G   +LLLL+Q  C+E  K KA  LLK+L
Sbjct: 372 DEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLL 398

BLAST of Sed0001282 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 4.1e-51
Identity = 134/429 (31.24%), Postives = 230/429 (53.61%), Query Frame = 0

Query: 26  ELTIPANFQCPISLDLMKDPVILSTGITYDRTSIETWIEAGNFR-CPITKQPLQSLDSIP 85
           E+ IP+ F CPISLD+MKDPVI+STGITYDR SIE W+ +G    CP+TKQ +   D  P
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 86  NHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIREILEKLSNAARREDGDRCKSLVEK 145
           NH +RR+IQ WC  N +YGIERIPTP+ P+   +I +++++ S++   +        +++
Sbjct: 64  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQ-----VKCLKR 123

Query: 146 IKMLGKESERNKKCI----ITNGTAIVLSSAFEAFSKPET-------------------F 205
           ++ +  E+  NK+C+    +    A ++S++ + ++ P +                   F
Sbjct: 124 LRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRF 183

Query: 206 EKSVEVLEEILSAMAIIFPLQEEAIKNL----KSETSLQSLVWFLRGGDLSGRRNSILVL 265
           + S  +++E LS +  +    E A+K+L    K    +++L   ++ G    R  + L+L
Sbjct: 184 DSSRSLMDEALSVLYHL-DTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLL 243

Query: 266 KEIISSYPEKVTKLAEIQGVLESLVKLIKEPICSSSKKASLFITYHIIASSSNPNGFTKS 325
           K+++         L E + +   +++++ + I   + ++++ I         N +     
Sbjct: 244 KKLLEVADPMQIILLE-RELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRH----K 303

Query: 326 LLQMGFLPLLLEMLVD----SERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKI 385
            ++ G + +++E+L+D    SER   E A+   D +C+  EGR E   H   I V+ KKI
Sbjct: 304 AVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKI 363

Query: 386 LRVSDLATELAVSIVWKLVKHEEDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKATEL 423
           LRVS + +E AV ++  + +       ++ + LQ+G   KL L+LQV C   TKEKA EL
Sbjct: 364 LRVSQITSERAVRVLLSVGRFCATPSLLQ-EMLQLGVVAKLCLVLQVSCGNKTKEKAKEL 420

BLAST of Sed0001282 vs. ExPASy TrEMBL
Match: A0A6J1K8U9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491172 PE=4 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 2.8e-196
Identity = 350/441 (79.37%), Postives = 395/441 (89.57%), Query Frame = 0

Query: 2   MIYSWRQWRATRRRMTRDGGVGIGELTIPANFQCPISLDLMKDPVILSTGITYDRTSIET 61
           MI SWR+ RATRR     GG  + ELTIP++F+CPISLD+MKDPV LSTGITYDR SIET
Sbjct: 1   MISSWRRRRATRRTARGTGGC-MRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIET 60

Query: 62  WIEAGNFRCPITKQPLQSLDSIPNHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIRE 121
           WIE GNF CPIT QPLQ+LDSIPNHNIR+MIQDWCV+N+AYG+ERIPTPRVP SP+QIR+
Sbjct: 61  WIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPASPVQIRD 120

Query: 122 ILEKLSNAARREDGDRCKSLVEKIKMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETF 181
           IL+K++ A RR DGD CKS VEKIKML KESERNKKCI+ NG + V+SSAF+A SK E  
Sbjct: 121 ILDKITAATRRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPL 180

Query: 182 EKSVEVLEEILSAMAIIFPLQEEAIKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEII 241
           EKS+E+LEE++S MAI+FPLQEE+IKNLKSE SLQ+L  F+RGGDLS RRNSIL+LKEII
Sbjct: 181 EKSIEILEEMISGMAIMFPLQEESIKNLKSEASLQALALFMRGGDLSRRRNSILILKEII 240

Query: 242 SSYPEKVTKLAEIQGVLESLVKLIKEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQM 301
           SSYP+K TKL EIQG LE LVKLIKEPICSSSKKASLFITYHIIASSSNP  FTK+LLQM
Sbjct: 241 SSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALLQM 300

Query: 302 GFLPLLLEMLVDSERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKILRVSDLAT 361
           GF+ LLLE LVD+ERS+CERALG FDGICES+EGREEAYAHALT+PVIVKKILRVSDLAT
Sbjct: 301 GFVSLLLETLVDAERSICERALGVFDGICESKEGREEAYAHALTMPVIVKKILRVSDLAT 360

Query: 362 ELAVSIVWKLVKH--EEDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKATELLKMLNS 421
           EL+VSIVWKLVKH  EE+DG VKV+ALQVGAFQKLLLLLQVGCSEWTKEKATELLK+LN+
Sbjct: 361 ELSVSIVWKLVKHESEEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNN 420

Query: 422 VHRNRLECIDSLDFKNLKRPF 441
           +HR+RLECIDSLDFK++KRPF
Sbjct: 421 LHRDRLECIDSLDFKDIKRPF 440

BLAST of Sed0001282 vs. ExPASy TrEMBL
Match: A0A6J1FWS8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447660 PE=4 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 2.8e-196
Identity = 352/441 (79.82%), Postives = 395/441 (89.57%), Query Frame = 0

Query: 2   MIYSWRQWRATRRRMTRDGGVGIGELTIPANFQCPISLDLMKDPVILSTGITYDRTSIET 61
           MI SWRQ RAT RR  R  G  + ELTIP++F+CPISLD+MKDPV LSTGITYDR SIET
Sbjct: 1   MISSWRQRRAT-RRTARGTGSDMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASIET 60

Query: 62  WIEAGNFRCPITKQPLQSLDSIPNHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIRE 121
           WIE GNF CPIT QPLQ+LDSIPNHNIR+MIQDWCV+N+AYG+ERIPTPRVPVSP+QIR+
Sbjct: 61  WIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPVSPVQIRD 120

Query: 122 ILEKLSNAARREDGDRCKSLVEKIKMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETF 181
           ILEK++ AARR DGD CKS VEKIKML KESERNKKCI+ NG + V+SSAF+A SK E  
Sbjct: 121 ILEKITAAARRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQEPL 180

Query: 182 EKSVEVLEEILSAMAIIFPLQEEAIKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEII 241
           EKS+E+LEE++SAMAI+FPLQEE+IKNLKSE SLQ+LV F++GGDLS RRNSIL+LK+II
Sbjct: 181 EKSIEILEEMISAMAIMFPLQEESIKNLKSEASLQALVLFMKGGDLSRRRNSILILKQII 240

Query: 242 SSYPEKVTKLAEIQGVLESLVKLIKEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQM 301
           SSYP+K TKL EIQG LE LVKLIKEPICSSSKKASLFITYHIIASSSNP  FTK LL+M
Sbjct: 241 SSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKVLLEM 300

Query: 302 GFLPLLLEMLVDSERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKILRVSDLAT 361
           GF+ LLLE LVD+ERSVCERALGAFDGICE +EGREEAYAHALT+PVIVKKILRVSDLAT
Sbjct: 301 GFVSLLLETLVDAERSVCERALGAFDGICERKEGREEAYAHALTMPVIVKKILRVSDLAT 360

Query: 362 ELAVSIVWKLVKH--EEDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKATELLKMLNS 421
           EL+VSIVWKLVKH  EE+DG  KV+ALQVGAFQKLLLLLQVGCSEWTKEKATELLK+LN+
Sbjct: 361 ELSVSIVWKLVKHESEEEDGRAKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNN 420

Query: 422 VHRNRLECIDSLDFKNLKRPF 441
           +HR+ LECIDSLDFK +KRPF
Sbjct: 421 LHRDSLECIDSLDFKGIKRPF 440

BLAST of Sed0001282 vs. ExPASy TrEMBL
Match: A0A0A0KMI8 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE=4 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.4e-195
Identity = 353/445 (79.33%), Postives = 392/445 (88.09%), Query Frame = 0

Query: 2   MIYSWRQWRATRRRMTRDGGVGIGELTIPANFQCPISLDLMKDPVILSTGITYDRTSIET 61
           MI SWR+ RAT RRM R G     ELTIP +F+CPISLDLMKDPV LSTGITYDR SIET
Sbjct: 1   MISSWRRRRAT-RRMARGGLSHTIELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIET 60

Query: 62  WIEAGNFRCPITKQPLQSLDSIPNHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIRE 121
           WIE GNF CP T QPLQ++DSIPNHNIR+MIQDWCV+NRAYGIERIPTPRVP SP Q+RE
Sbjct: 61  WIEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRE 120

Query: 122 ILEKLSNAARREDGDRCKSLVEKIKMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETF 181
           ILEK++ AARR D D CKS+VEKIK LGKESERNKKCII NGT   LSS FEAFS PETF
Sbjct: 121 ILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNTLSSIFEAFSNPETF 180

Query: 182 EKSVEVLEEILSAMAIIFPLQEEAIKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEII 241
           EK +E+LEEILSAM + FPLQEE+IK+LKSETSLQSLVWFL+GGD+SGRRNS+LVLKEII
Sbjct: 181 EKRIEILEEILSAMTVAFPLQEESIKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEII 240

Query: 242 SSYPEKVTKLAEIQGVLESLVKLIKEPICSSSKKASLFITYHIIA---SSSNPNGFTKSL 301
           SSYPEKV +L EIQG LE L+KLIK+PICSSS+KASLFITYH+IA   SSSN   FTK L
Sbjct: 241 SSYPEKVDELGEIQGALEGLIKLIKDPICSSSRKASLFITYHVIASTSSSSNRERFTKEL 300

Query: 302 LQMGFLPLLLEMLVDSERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKILRVSD 361
           LQMG + LLLE LVD+ERSVCERALGAFDGICE+++GREEAYAHALT+PVIVKKILRVSD
Sbjct: 301 LQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360

Query: 362 LATELAVSIVWKLVKH---EEDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKATELLK 421
           LATEL+V IVWKLVKH   EE+DG +KV+ALQVGAFQKLLLLLQVGCSEWTKEKATELLK
Sbjct: 361 LATELSVCIVWKLVKHESKEEEDGGIKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLK 420

Query: 422 MLNSVHRNRLECIDSLDFKNLKRPF 441
           +LN++HR+RLECIDSLDFK+LKR F
Sbjct: 421 LLNNLHRDRLECIDSLDFKDLKRTF 444

BLAST of Sed0001282 vs. ExPASy TrEMBL
Match: A0A6J1D0F8 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111015879 PE=4 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 4.5e-194
Identity = 349/440 (79.32%), Postives = 389/440 (88.41%), Query Frame = 0

Query: 2   MIYSWRQWRATRRRMTRDGGVGIGELTIPANFQCPISLDLMKDPVILSTGITYDRTSIET 61
           MI SWR+ RATR+R     G G  ELTIP +F+CPISLDLMKDPV LSTGITYDR SIET
Sbjct: 1   MISSWRRRRATRQREVE--GRGAVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIET 60

Query: 62  WIEAGNFRCPITKQPLQSLDSIPNHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIRE 121
           WIE GNF CPIT QPL SLD IPNHNIR+MIQDWCVDNRAYGIERIPTPRVP+S +Q+ E
Sbjct: 61  WIEGGNFSCPITNQPLVSLDPIPNHNIRKMIQDWCVDNRAYGIERIPTPRVPLSSVQLLE 120

Query: 122 ILEKLSNAARREDGDRCKSLVEKIKMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETF 181
           IL K++ A RR D + C+SLVE+IK LGKESERNKKCII NGT  VL+SAFE FS+PETF
Sbjct: 121 ILSKITAATRRGDAEECQSLVERIKRLGKESERNKKCIIANGTGHVLASAFEVFSRPETF 180

Query: 182 EKSVEVLEEILSAMAIIFPLQEEAIKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEII 241
           EKS EVL E+LSA+A +FPLQEEAIK+LKSE SLQSLVWFL+GGDLSGRR+S+LVLKEII
Sbjct: 181 EKSNEVLAEVLSAIATVFPLQEEAIKHLKSEASLQSLVWFLKGGDLSGRRSSVLVLKEII 240

Query: 242 SSYPEKVTKLAEIQGVLESLVKLIKEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQM 301
           SSYPEKVT+LAEIQG LE  VKLIKEPIC SS+KASL ITYH IASSSNP  FTK+LLQM
Sbjct: 241 SSYPEKVTELAEIQGALEGFVKLIKEPICPSSRKASLLITYHAIASSSNPERFTKALLQM 300

Query: 302 GFLPLLLEMLVDSERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKILRVSDLAT 361
           GF+ LLLE LVD+ERSVCERALGAFDGICES+EGREE Y HALT+PVIVKKILRVSD+AT
Sbjct: 301 GFVSLLLETLVDAERSVCERALGAFDGICESKEGREEVYGHALTMPVIVKKILRVSDVAT 360

Query: 362 ELAVSIVWKLVKHE-EDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKATELLKMLNSV 421
           E++V+IVWKLVKHE  +DG VKV+ALQVGAFQKLLLLLQVGCSEWTKEKATELLK+LN +
Sbjct: 361 EISVAIVWKLVKHESREDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNGL 420

Query: 422 HRNRLECIDSLDFKNLKRPF 441
           HR+RLECI+SLDFK+LKRPF
Sbjct: 421 HRDRLECIESLDFKDLKRPF 438

BLAST of Sed0001282 vs. ExPASy TrEMBL
Match: A0A6J1FPJ6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447642 PE=4 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 5.9e-194
Identity = 351/449 (78.17%), Postives = 396/449 (88.20%), Query Frame = 0

Query: 1   MMIYSWRQWRATRRRMTRDGGVGIG---ELTIPANFQCPISLDLMKDPVILSTGITYDRT 60
           MM++SWR+ RAT RRM R GG G G   ELTIP++F CPISLDLMKDPV +STGITYDR 
Sbjct: 1   MMMFSWRRRRAT-RRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRA 60

Query: 61  SIETWIEAGNFRCPITKQPLQSLDSIPNHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPM 120
           +IETWIE GNF CP T QPL ++DSIPNHNIR+MIQ WCV+NRA+GIERIPTPRVP SP+
Sbjct: 61  NIETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPV 120

Query: 121 QIREILEKLSNAARREDGDRCKSLVEKIKMLGKESERNKKCIITNGTAIVLSSAFEAFSK 180
           Q+REIL+K++ A RRED + CKSLVEKIK+L +ESERNKKCII NGT  VLSSAFEAFSK
Sbjct: 121 QVREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSK 180

Query: 181 PETFEKSVEVLEEILSAMAIIFPLQEEAIKNLKSETSLQSLVWFLRGGDLSGRRNSILVL 240
           PE FEK VE+LEEILS MA+IFPLQEEAIK+LKSE S+Q+LVWF+RG D S RR+SILVL
Sbjct: 181 PERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVL 240

Query: 241 KEIISSYPEKVTKLAEIQGVLESLVKLIKEPICSSSKKASLFITYHIIASSSNPNGFTKS 300
           KEIISSYPEKV++L EIQGVLE L+KLIKEPICSSSKKASLFITYH IASSSN  GFTK+
Sbjct: 241 KEIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKA 300

Query: 301 LLQMGFLPLLLEMLVDSERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKILRVS 360
            LQMG +PLLLE LVD+ERSVCERALG FDGICE+++GREEAYAHALT+PVIVKKILRVS
Sbjct: 301 FLQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVS 360

Query: 361 DLATELAVSIVWKLVKH------EEDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKAT 420
           DL TEL+VSIVWKLVKH      E++DG VKV+ALQVGAFQKLLLLLQVGCSEWTKEKAT
Sbjct: 361 DLVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKAT 420

Query: 421 ELLKMLNSVHRNRLECIDSLDFKNLKRPF 441
           ELLK+LN++HR RLECIDSLDFK+LKR F
Sbjct: 421 ELLKLLNNLHRERLECIDSLDFKDLKRTF 448

BLAST of Sed0001282 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.5e-104
Identity = 203/416 (48.80%), Postives = 278/416 (66.83%), Query Frame = 0

Query: 26  ELTIPANFQCPISLDLMKDPVILSTGITYDRTSIETWIEAGNFRCPITKQPLQSLDSIPN 85
           E+TIP  FQCPIS+DLMKDPVI+STGITYDR SIETWI +GN  CP+T   L + D IPN
Sbjct: 28  EITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPN 87

Query: 86  HNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIREILEKLSNAARREDGDRCKSLVEKI 145
           H IR+MIQ WCV+  +  I+RIPTPRVP+ P ++ EI  KLS+A RR D ++C  ++EKI
Sbjct: 88  HTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKI 147

Query: 146 KMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETFEKSVEVLEEILSAMAIIFPLQEEA 205
           K LG ESE+N+KC+  N    VL   F+ FS     EK   +L EILS +  +FP+  E 
Sbjct: 148 KKLGDESEKNRKCVNENSVGWVLCDCFDKFSGD---EKLTFMLNEILSLLTWMFPIGLEG 207

Query: 206 IKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEIISSYPEKVTKLAEIQGVLESLVKLI 265
           I  L S TS + +   L+  D S R+N+  ++KEI+S    +V   A   GV E+LVKLI
Sbjct: 208 ISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKLI 267

Query: 266 KEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQMGFLPLLLEMLVDSERSVCERALGA 325
           ++ + SSS K+SL   Y ++             L++G + + +EM+VD+E SVCE+AL  
Sbjct: 268 RDSVSSSSTKSSLIAIYQMVLQKPE---IASEFLEIGLVSITVEMIVDAENSVCEKALAV 327

Query: 326 FDGICESEEGREEAYAHALTIPVIVKKILRVSDLATELAVSIVWKLVKHEEDDGWVKVDA 385
            D ICE+E GREE   +AL +P++VKKI +VS+LAT  ++S++ KL K    +     DA
Sbjct: 328 LDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWK--TGNTVAVEDA 387

Query: 386 LQVGAFQKLLLLLQVGCSEWTKEKATELLKMLNSVHRNRLECIDSL-DFKNLKRPF 441
           +++GAFQK+LL+LQVG  E TKEKATELLKM+N+  +   +C+DSL +FK +K+PF
Sbjct: 388 VRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYIKKPF 435

BLAST of Sed0001282 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 329.3 bits (843), Expect = 4.6e-90
Identity = 189/419 (45.11%), Postives = 265/419 (63.25%), Query Frame = 0

Query: 26  ELTIPANFQCPISLDLMKDPVILSTGITYDRTSIETWIEAGNFRCPITKQPLQSLDSIPN 85
           E+TIP+ FQCPIS +LMKDPVI+++GITYDR +IE W E+G   CP+T   L SL+ IPN
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLEQIPN 89

Query: 86  HNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIREILEKLSNAARREDGDRCKSLVEKI 145
           H IRRMIQ WC  +   GIERIPTPRVPV+  Q+ EI E+LS A RR D   C  +V K+
Sbjct: 90  HTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMVTKM 149

Query: 146 KMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETFEKSVEVLEEILSAMAIIFPLQEEA 205
             LGKESERN+KC+  NG  +VL   F+AFS  E    S+ +LEE +S +  + P+  E 
Sbjct: 150 TRLGKESERNRKCVKENGAGLVLCVCFDAFS--ENANASL-LLEETVSVLTWMLPIGLEG 209

Query: 206 IKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEIISSYPEKVTKLAEIQGVLESLVKLI 265
              L + +S   LV  LR GD    +N+  ++KE++      V  L +I GV E+ +K I
Sbjct: 210 QSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAFMKSI 269

Query: 266 KEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQMGFLPLLLEMLVDSERSVCERALGA 325
                 S+   SL   +H+I ++          L++  + + +EMLVDSE SVCE+AL  
Sbjct: 270 NR---DSTCVNSLISIHHMILTNQET---VSRFLELDLVNITVEMLVDSENSVCEKALTV 329

Query: 326 FDGICESEEGREEAYAHALTIPVIVKKILRVSDLATELAVSIVWKLVKHEEDDGWVKVDA 385
            + ICE++EGRE+   + L IP++VKKIL++S+   +  VS++WK+ K    DG    +A
Sbjct: 330 LNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK--SGDGSEVEEA 389

Query: 386 LQVGAFQKLLLLLQVGCSEWTKEKATELLKMLNSVHRNR----LECIDSLDFKNLKRPF 441
           L++GAF+KL+++LQVGC E TKEK TELLKM+N V +           S++FK++K+PF
Sbjct: 390 LRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEFKHVKKPF 431

BLAST of Sed0001282 vs. TAIR 10
Match: AT1G66160.2 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 298.1 bits (762), Expect = 1.1e-80
Identity = 179/419 (42.72%), Postives = 251/419 (59.90%), Query Frame = 0

Query: 26  ELTIPANFQCPISLDLMKDPVILSTGITYDRTSIETWIEAGNFRCPITKQPLQSLDSIPN 85
           E+TIP+ FQCPIS +LMKDP                W E+G   CP+T   L SL+ IPN
Sbjct: 30  EITIPSQFQCPISYELMKDP----------------WFESGYQTCPVTNTVLTSLEQIPN 89

Query: 86  HNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIREILEKLSNAARREDGDRCKSLVEKI 145
           H IRRMIQ WC  +   GIERIPTPRVPV+  Q+ EI E+LS A RR D   C  +V K+
Sbjct: 90  HTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMVTKM 149

Query: 146 KMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETFEKSVEVLEEILSAMAIIFPLQEEA 205
             LGKESERN+KC+  NG  +VL   F+AFS  E    S+ +LEE +S +  + P+  E 
Sbjct: 150 TRLGKESERNRKCVKENGAGLVLCVCFDAFS--ENANASL-LLEETVSVLTWMLPIGLEG 209

Query: 206 IKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEIISSYPEKVTKLAEIQGVLESLVKLI 265
              L + +S   LV  LR GD    +N+  ++KE++      V  L +I GV E+ +K I
Sbjct: 210 QSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAFMKSI 269

Query: 266 KEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQMGFLPLLLEMLVDSERSVCERALGA 325
                 S+   SL   +H+I ++          L++  + + +EMLVDSE SVCE+AL  
Sbjct: 270 NR---DSTCVNSLISIHHMILTNQET---VSRFLELDLVNITVEMLVDSENSVCEKALTV 329

Query: 326 FDGICESEEGREEAYAHALTIPVIVKKILRVSDLATELAVSIVWKLVKHEEDDGWVKVDA 385
            + ICE++EGRE+   + L IP++VKKIL++S+   +  VS++WK+ K    DG    +A
Sbjct: 330 LNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK--SGDGSEVEEA 389

Query: 386 LQVGAFQKLLLLLQVGCSEWTKEKATELLKMLNSVHRNR----LECIDSLDFKNLKRPF 441
           L++GAF+KL+++LQVGC E TKEK TELLKM+N V +           S++FK++K+PF
Sbjct: 390 LRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEFKHVKKPF 415

BLAST of Sed0001282 vs. TAIR 10
Match: AT3G02840.1 (ARM repeat superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 1.0e-68
Identity = 151/346 (43.64%), Postives = 227/346 (65.61%), Query Frame = 0

Query: 104 IERIPTPRVPVSPMQIREILEKLSNAARREDGDRCKSLVEKIKMLGKESERNKKCIITNG 163
           IERI +PRV ++P    EI  +L NAA RE+   C  +V KIK LG+  + NKKC++ NG
Sbjct: 40  IERIQSPRVLLTPRDAVEISRRLQNAAAREEYAECLEIVSKIKNLGRGGDTNKKCLVQNG 99

Query: 164 TAIVLSSAFEAFSKPETFEKSVEVLEEILSAMAIIFPL-QEEAIKNLKSETSLQSLVWFL 223
           + + LSS FE F+     +  + +LEEIL  ++   PL + E    + S  SL  LV FL
Sbjct: 100 SVLALSSCFERFAAAR--DGHMRLLEEILFVLSSWLPLNRSEGFNKMGSTASLNCLVRFL 159

Query: 224 RGGDLSGRRNSILVLKEIISSYPEKVTKLAEIQGVLESLVKLIKEPICSSSKKASLFITY 283
            G D   R+N+   ++E+I+     V  L +++G  E LVK+I++ + +SS KASL + Y
Sbjct: 160 NGKDAKTRQNAAFCIREVIAVDKRYVYALTDVEGACEGLVKIIRDSVSTSSTKASLMVIY 219

Query: 284 HIIASSSNPNGFTKSLLQMGFLPLLLEMLV-DSERSVCERALGAFDGICESEEGREEAYA 343
             I+S+      T+  +++G + L+ EM+V ++E+SVCER+L   +  C++E+G+E+   
Sbjct: 220 RAISSNDK---ITEKFVKLGLVELITEMMVNNAEKSVCERSLVVLNATCDNEQGKEDVLR 279

Query: 344 HALTIPVIVKKILRVSDLATELAVSIVWKLVKHEED---DGWVKVDALQVGAFQKLLLLL 403
           +AL +P++VKKILRVSDLAT+ +VSI+WKL K+++D   D  + V+ALQVGAF+KLL+LL
Sbjct: 280 NALIVPLLVKKILRVSDLATQCSVSILWKLWKNKKDGECDDRLLVEALQVGAFEKLLVLL 339

Query: 404 QVGCSEWTKEKATELLKMLNSVHRNRLE---CID-SLDFKNLKRPF 441
           QVGC + TKEKA+ELL+ LN   RN +E   C+D S+  KN+K+ F
Sbjct: 340 QVGCEDKTKEKASELLRNLNRC-RNEIEKTNCVDSSMHLKNVKKSF 379

BLAST of Sed0001282 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 213.0 bits (541), Expect = 4.8e-55
Identity = 134/403 (33.25%), Postives = 223/403 (55.33%), Query Frame = 0

Query: 20  GGVGIGELTIPANFQCPISLDLMKDPVILSTGITYDRTSIETWIEAGNFR-CPITKQPLQ 79
           GG+   E+ IP  F CPISL++MKDPVI+STGITYDR SIE W+ AG    CP+TKQ + 
Sbjct: 3   GGIMDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDIT 62

Query: 80  SLDSIPNHNIRRMIQDWCVDNRAYGIERIPTPRVPVSPMQIREILEKLSNAARREDGDRC 139
             D  PNH +RR+IQ WC  N +YG+ERIPTPR P+   +I +++    +A+  E+  +C
Sbjct: 63  DADLTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIR--DSASSHENQVKC 122

Query: 140 KSLVEKIKMLGKESERNKKCIITNGTAIVLSSAFEAFSKPETF-EKSVEVLEEILSAMAI 199
              +++++ +  E+  NK+C+   G    L++     S+  +  ++++ +L  + ++  +
Sbjct: 123 ---LKRLRQIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETV 182

Query: 200 IFPLQEEAIKNLKSETSLQSLVWFLRGGDLSGRRNSILVLKEIISSYPEKVTKLAEIQGV 259
           +    +  + N K    ++SL   ++ G    R  + L+LK I+    + +  +     V
Sbjct: 183 L----KNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNIL-EVADPMQSMTLKPEV 242

Query: 260 LESLVKLIKEPICSSSKKASLFITYHIIASSSNPNGFTKSLLQMGFLPLLLEMLVD---- 319
              +V+++ + I   + KA++ I  +I     N +      ++ G + +++E+L+D    
Sbjct: 243 FTEVVQILDDRISQKATKAAMHILVNICPWGRNRH----KAVEAGVISVIIELLMDESFT 302

Query: 320 SERSVCERALGAFDGICESEEGREEAYAHALTIPVIVKKILRVSDLATELAVSIVWKLVK 379
           SER   E A+   D +C+  EGR E   H   I V+ KKILRVS  A++ AV ++  + +
Sbjct: 303 SERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGR 362

Query: 380 HEEDDGWVKVDALQVGAFQKLLLLLQVGCSEWTKEKATELLKM 417
                  +  + LQ+G   KL L+LQV C   TKEKA ELLK+
Sbjct: 363 FCATPALLH-EMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKL 390

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6599923.13.4e-19679.46U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022942705.15.9e-19679.82U-box domain-containing protein 21-like [Cucurbita moschata][more]
XP_022995728.15.9e-19679.37U-box domain-containing protein 21-like [Cucurbita maxima][more]
KAG7030606.17.7e-19679.46U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_023512745.11.0e-19579.46U-box domain-containing protein 21-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q5PNY62.1e-10348.80U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
Q9C8D16.5e-8945.11U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1... [more]
Q84TG36.8e-5433.25E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9FXA41.1e-5133.67U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Q9SVC64.1e-5131.24E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1K8U92.8e-19679.37RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491172 P... [more]
A0A6J1FWS82.8e-19679.82RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447660... [more]
A0A0A0KMI81.4e-19579.33RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE... [more]
A0A6J1D0F84.5e-19479.32RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101587... [more]
A0A6J1FPJ65.9e-19478.17RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447642... [more]
Match NameE-valueIdentityDescription
AT5G37490.11.5e-10448.80ARM repeat superfamily protein [more]
AT1G66160.14.6e-9045.11CYS, MET, PRO, and GLY protein 1 [more]
AT1G66160.21.1e-8042.72CYS, MET, PRO, and GLY protein 1 [more]
AT3G02840.11.0e-6843.64ARM repeat superfamily protein [more]
AT2G35930.14.8e-5533.25plant U-box 23 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 32..95
e-value: 5.7E-25
score: 99.0
IPR003613U box domainPFAMPF04564U-boxcoord: 29..100
e-value: 2.0E-19
score: 69.5
IPR003613U box domainPROSITEPS51698U_BOXcoord: 28..102
score: 37.472153
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 117..429
e-value: 2.4E-19
score: 71.4
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 28..102
e-value: 1.9E-27
score: 97.1
NoneNo IPR availablePANTHERPTHR22849:SF139ARM REPEAT SUPERFAMILY PROTEINcoord: 22..440
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 22..440
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 33..75
e-value: 3.71725E-23
score: 89.6002
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 20..105
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 121..417

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0001282.1Sed0001282.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity