Sed0000677 (gene) Chayote v1

BLAST of Sed0000677 vs. NCBI nr
Match: XP_004152537.2 (pectate lyase isoform X1 [Cucumis sativus] >KGN64253.1 hypothetical protein Csa_013613 [Cucumis sativus])

HSP 1 Score: 773.5 bits (1996), Expect = 1.1e-219
Identity = 353/439 (80.41%), Postives = 403/439 (91.80%), Query Frame = 0

Query: 62  MRETECSIFRIKLLMIFAFVMIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEVA 121
           MRET+ +I  IKLLMIF FVMIFPTLKANI +FDEVWQ+R+ EA+KA+F+A++P PEE  
Sbjct: 1   MRETKTTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAIEAQKASFEAYEPHPEEET 60

Query: 122 NNLNKEVNSALYGGNKTRRNLGKYNGPCLATNPIDRCWRCDKNWYKNRKKLADCALGFGR 181
           NN NK+V+ +L GGN TRR+L KY GPCLATNPIDRCWRCD+NW +NRKKLADCALGFGR
Sbjct: 61  NNFNKQVHRSLDGGNNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGR 120

Query: 182 GTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFTDN 241
            T GGKDG+ YVVRDSSD+D++NPKPGTLRHAVIQ+RPLWI FA DMVIRL EELI TD+
Sbjct: 121 RTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIVTDD 180

Query: 242 KTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSDGD 301
           KTLDGRGANVHIA+GGQ+TLQFVKNIIIHN+H+HDIKAGNGG+IRDSVSHYGFRTRSDGD
Sbjct: 181 KTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGMIRDSVSHYGFRTRSDGD 240

Query: 302 GISLFGASKVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSDDR 361
           GIS+FGAS+VWIDHVS+ +C DGLIDA+MAST ITISNCHFTHHNDV+LLGAS+GYS+D+
Sbjct: 241 GISMFGASRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASNGYSNDQ 300

Query: 362 IMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFTAP 421
           IMQVTLAFNHFGKGLVQRMPRCRWGF HVVNND+THW MYAIGGS NPTIISQGNRF AP
Sbjct: 301 IMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAP 360

Query: 422 PNPNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRGHSKKDVISAKPGTY 481
           PNPNCKEVTKRV+AP+S W+SWNWRSEGDL +NGAFF+QSGNP++ +SKKDVI +KPGT+
Sbjct: 361 PNPNCKEVTKRVYAPESEWRSWNWRSEGDLMMNGAFFIQSGNPIKRYSKKDVIHSKPGTF 420

Query: 482 VTRLTRFSGTLHCRRNQPC 501
           VTRLTRF+G L C++NQPC
Sbjct: 421 VTRLTRFAGPLKCKKNQPC 439

BLAST of Sed0000677 vs. NCBI nr
Match: XP_038894610.1 (pectate lyase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 769.6 bits (1986), Expect = 1.6e-218
Identity = 355/439 (80.87%), Postives = 398/439 (90.66%), Query Frame = 0

Query: 62  MRETECSIFRIKLLMIFAFVMIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEVA 121
           MRETE +I  IKLLMIF FVMIFPTLKANI +FDEVWQ+R++EAKKA+F+A+QP PEEV 
Sbjct: 1   MRETETTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAEEAKKASFEAYQPHPEEVT 60

Query: 122 NNLNKEVNSALYGGNKTRRNLGKYNGPCLATNPIDRCWRCDKNWYKNRKKLADCALGFGR 181
           +N NK+V+ +L G N TRR+L KYNGPCLATNPIDRCWRCDK+W KNRKKLADCALGFGR
Sbjct: 61  SNFNKQVHKSLDGANNTRRHLRKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGR 120

Query: 182 GTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFTDN 241
           GT GGK+G  YVVRDSSD+D+LNPKPGTLR+ VIQDRPLWI FA DMVIRL EELI TDN
Sbjct: 121 GTTGGKNGRIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDN 180

Query: 242 KTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSDGD 301
           KTLDGRGANVHIA+GGQ+TLQFVKNIIIHN+H+HDIKAGNGG+IRDSV+HYGFRT SDGD
Sbjct: 181 KTLDGRGANVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGD 240

Query: 302 GISLFGASKVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSDDR 361
           GIS+FGASKVWIDHVS+ +C DGLIDA+MASTGITISNCHFT HNDV+LLGASD YS+D+
Sbjct: 241 GISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQ 300

Query: 362 IMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFTAP 421
           IMQVTLAFNHFGKGLVQRMPRCRWGF HVVNND+THW MYAIGGS NPTIISQGNRF AP
Sbjct: 301 IMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAP 360

Query: 422 PNPNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRGHSKKDVISAKPGTY 481
           PNPN KEVTKR +A +S W+SWNWRSEGDL +NGAFF+QSGNP+R +SKKDVI +KPGT+
Sbjct: 361 PNPNSKEVTKRAYASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRRYSKKDVIHSKPGTF 420

Query: 482 VTRLTRFSGTLHCRRNQPC 501
           VTRLTRF+G L C +NQPC
Sbjct: 421 VTRLTRFAGPLKCIKNQPC 439

BLAST of Sed0000677 vs. NCBI nr
Match: TYJ99102.1 (pectate lyase-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 764.6 bits (1973), Expect = 5.3e-217
Identity = 350/439 (79.73%), Postives = 397/439 (90.43%), Query Frame = 0

Query: 62  MRETECSIFRIKLLMIFAFVMIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEVA 121
           MRET+ +I  IKLLMIF FVMIFPTLKANI +FDEVWQ+R+ EAKKA+ +A+QP PEEV 
Sbjct: 1   MRETKTTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAIEAKKASLEAYQPHPEEVT 60

Query: 122 NNLNKEVNSALYGGNKTRRNLGKYNGPCLATNPIDRCWRCDKNWYKNRKKLADCALGFGR 181
           NN NK+V+ +L G N TRR+L KY GPCLATNPIDRCWRCD+NW +NRKKLADCALGFGR
Sbjct: 61  NNFNKQVHRSLDGANNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGR 120

Query: 182 GTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFTDN 241
           GT GGKDG+ YVVRDSSD+D++NPKPGTLRHAVIQ+RPLWI FA DMVIRL EELI TDN
Sbjct: 121 GTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIMTDN 180

Query: 242 KTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSDGD 301
           KTLDGRGANVHIA+GGQ+TLQFV+NIIIHN+H+HDIKAGNGG IRDSVSHYG+RT+SDGD
Sbjct: 181 KTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGD 240

Query: 302 GISLFGASKVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSDDR 361
           GIS+FG+S+VWIDHVS+ +C DGLIDA+MAST ITISNCHFTHHNDV+LLGASD YS+D+
Sbjct: 241 GISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYSNDK 300

Query: 362 IMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFTAP 421
           IMQVTLAFNHFGKGLVQRMPRCRWGF HVVNND+THW MYAIGGS NPTIISQGNRF AP
Sbjct: 301 IMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAP 360

Query: 422 PNPNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRGHSKKDVISAKPGTY 481
           PNPN KEVTKRV+A +  W+SWNWRSEGDL +NGAFF+QSGNP+R +SKKDVI +KPGT+
Sbjct: 361 PNPNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRRYSKKDVIHSKPGTF 420

Query: 482 VTRLTRFSGTLHCRRNQPC 501
           VTRLTRF+G L C+ NQPC
Sbjct: 421 VTRLTRFAGPLKCKMNQPC 439

BLAST of Sed0000677 vs. NCBI nr
Match: XP_022994124.1 (pectate lyase-like [Cucurbita maxima])

HSP 1 Score: 761.5 bits (1965), Expect = 4.5e-216
Identity = 354/440 (80.45%), Postives = 395/440 (89.77%), Query Frame = 0

Query: 62  MRETECSIFRIK-LLMIFAFVMIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEV 121
           MRE E +I   K LL+IFAF +IFPTL+A+I +FDEVWQ+R++EAKKAA +A+QP PEEV
Sbjct: 1   MRELESNIVGAKLLLLIFAFAIIFPTLEADIADFDEVWQQRAQEAKKAALEAYQPHPEEV 60

Query: 122 ANNLNKEVNSALYGGNKTRRNLGKYNGPCLATNPIDRCWRCDKNWYKNRKKLADCALGFG 181
             N N+ VN  L   N TRR+L KYNGPCLATNPIDRCWRCDKNW KNRKKLADCALGFG
Sbjct: 61  TENFNEHVNGDLDDANNTRRHLRKYNGPCLATNPIDRCWRCDKNWAKNRKKLADCALGFG 120

Query: 182 RGTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFTD 241
           RGT GGK+G FYVV+DSSD+DML+PKPGTLR+AVIQDRPLWITFA DMVIRL EELI TD
Sbjct: 121 RGTTGGKNGRFYVVQDSSDNDMLHPKPGTLRYAVIQDRPLWITFAHDMVIRLAEELIMTD 180

Query: 242 NKTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSDG 301
           NKTLDGRGANVHIA+GGQ+TLQFVKNIIIHN+H+HDIKAGNGGLIRDSV+HYG RTRSDG
Sbjct: 181 NKTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLIRDSVNHYGIRTRSDG 240

Query: 302 DGISLFGASKVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSDD 361
           DGIS+FGAS VWIDHVS+ +C DGLIDA+MAST ITISNCHF HHNDV+LLGASD +SDD
Sbjct: 241 DGISIFGASNVWIDHVSMSNCQDGLIDAVMASTAITISNCHFAHHNDVILLGASDSHSDD 300

Query: 362 RIMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFTA 421
           +IMQVTLAFNHFGKGLVQRMPRCRWGF HVVNND+THW MYAIGGS NPTIISQGNRF A
Sbjct: 301 QIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSRNPTIISQGNRFIA 360

Query: 422 PPNPNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRGHSKKDVISAKPGT 481
           PP+PNCKEVTKRV+AP+S WK+WNWRSEGDL +NGAFFV+SGNP+R +SKKDVI AKPGT
Sbjct: 361 PPDPNCKEVTKRVYAPESDWKTWNWRSEGDLLMNGAFFVESGNPIRKYSKKDVIHAKPGT 420

Query: 482 YVTRLTRFSGTLHCRRNQPC 501
           +VTRLTRF+G L C RNQPC
Sbjct: 421 FVTRLTRFAGPLKCTRNQPC 440

BLAST of Sed0000677 vs. NCBI nr
Match: XP_022954990.1 (pectate lyase-like [Cucurbita moschata])

HSP 1 Score: 754.2 bits (1946), Expect = 7.1e-214
Identity = 349/441 (79.14%), Postives = 395/441 (89.57%), Query Frame = 0

Query: 62  MRETECSI--FRIKLLMIFAFVMIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEE 121
           MRE + +I   ++ LL+IFAF +IFP+L+A I +FDEVWQ+R++EAKKAA +A+QP PEE
Sbjct: 1   MRELDSNIAGAKLLLLLIFAFAIIFPSLEAGIADFDEVWQQRAQEAKKAALEAYQPRPEE 60

Query: 122 VANNLNKEVNSALYGGNKTRRNLGKYNGPCLATNPIDRCWRCDKNWYKNRKKLADCALGF 181
           V  + N+ VN  L   N TRR+L KYNGPCLATNPIDRCWRCDKNW KNRKKLADCALGF
Sbjct: 61  VTESFNEHVNGDLDDANNTRRHLRKYNGPCLATNPIDRCWRCDKNWSKNRKKLADCALGF 120

Query: 182 GRGTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFT 241
           GRGT GGK+G FY+V+DSSD+DML+PKPGTLR+AVIQDRPLWITFA DMVIRL EELI T
Sbjct: 121 GRGTTGGKNGRFYIVQDSSDNDMLHPKPGTLRYAVIQDRPLWITFAHDMVIRLAEELIMT 180

Query: 242 DNKTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSD 301
           DNKTLDGRGANVHIA+GGQ+TLQFVKNIIIHN+H+HDIKAGNGGLIRDSV+HYG RTRSD
Sbjct: 181 DNKTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLIRDSVNHYGIRTRSD 240

Query: 302 GDGISLFGASKVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSD 361
           GDGIS+FGAS VWIDHVS+ +C DGLIDA+MAST ITISNCHF HHNDV+LLGASD +SD
Sbjct: 241 GDGISMFGASNVWIDHVSMSNCQDGLIDAVMASTAITISNCHFAHHNDVILLGASDSHSD 300

Query: 362 DRIMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFT 421
           D+IMQVTLAFNHFGKGLVQRMPRCRWGF HVVNND+THW MYAIGGS NPTIISQGNRF 
Sbjct: 301 DQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSRNPTIISQGNRFI 360

Query: 422 APPNPNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRGHSKKDVISAKPG 481
           APP+PNCKEVTKRV+AP+S WK+WNWRSEGDL +NGAFFVQSGNP+R +SKKDVI AKPG
Sbjct: 361 APPDPNCKEVTKRVYAPESEWKTWNWRSEGDLLMNGAFFVQSGNPIRKYSKKDVIHAKPG 420

Query: 482 TYVTRLTRFSGTLHCRRNQPC 501
           T+VTRLTRF+G L C RN+PC
Sbjct: 421 TFVTRLTRFAGPLKCIRNKPC 441

BLAST of Sed0000677 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 4.7e-160
Identity = 272/435 (62.53%), Postives = 321/435 (73.79%), Query Frame = 0

Query: 72  IKLLMIFAFVMIF----PTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEVANNLNKE 131
           +K    F + ++F        ANI EFDE WQ++SK A+  A  A+ P PEEV N+ NK 
Sbjct: 1   MKAAQFFLYSLLFFASAALSSANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKA 60

Query: 132 VNSALYGGNKTRRNLGKYN-GPCLATNPIDRCWRCDKNWYKNRKKLADCALGFGRGTIGG 191
           V+S+ + GN TRRNL     G CLATNPIDRCWRC KNW  NRK L  C  GFGR T GG
Sbjct: 61  VHSS-FEGNSTRRNLRTNKLGQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGG 120

Query: 192 KDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFTDNKTLDG 251
             GE YVV D SD  + +PK GTLR  VIQDRPLWI F   MVIRL +ELI  ++KT+DG
Sbjct: 121 AAGEIYVVTDPSDDSLTDPKFGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDG 180

Query: 252 RGANVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSDGDGISLF 311
           RGANV IA G Q+T+QFV N+IIH +H+HDIK G GGLIRDS  H G RTRSDGDGIS+ 
Sbjct: 181 RGANVQIAGGAQLTVQFVHNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISII 240

Query: 312 GASKVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSDDRIMQVT 371
           G+S +WIDHVSL  C+DGLID I+ ST ITISNCH T H+DV+LLGASD Y+ D IMQVT
Sbjct: 241 GSSNIWIDHVSLARCSDGLIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVT 300

Query: 372 LAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFTAPPNPNC 431
           +AFNHFG+GLVQRMPRCR+GF HVVNND+THW MYA+GGS +PTIISQGNR+ AP     
Sbjct: 301 VAFNHFGRGLVQRMPRCRYGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAA 360

Query: 432 KEVTKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRG-HSKKDVISAKPGTYVTRL 491
           KEVTKR +A  + W  W W+S+GDL V+GAFFV+SG P    +SKKD+I AKPGT+V RL
Sbjct: 361 KEVTKRDYAEPAEWSKWTWKSQGDLFVSGAFFVESGGPFENKYSKKDLIKAKPGTFVQRL 420

Query: 492 TRFSGTLHCRRNQPC 501
           TRFSG L+C+ N  C
Sbjct: 421 TRFSGALNCKENMEC 434

BLAST of Sed0000677 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 2.9e-157
Identity = 261/440 (59.32%), Postives = 322/440 (73.18%), Query Frame = 0

Query: 74  LLMIFAFVMIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEVANNLNKEVNSAL- 133
           L +   F  I P+L A+IG +DEVW+ R++EAK+ A + ++P PE V    N+++   + 
Sbjct: 11  LFLCITFATIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLRDTMK 70

Query: 134 -------YGGNKTRRNLG--KYNGPCLATNPIDRCWRCDKNWYKNRKKLADCALGFGRGT 193
                     N TRR LG  KY GPC+ TNPID+CWRCD NW  NRKKLADCA+GFG   
Sbjct: 71  ELKKVKGTHNNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSKA 130

Query: 194 IGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFTDNKT 253
           IGGKDGEFYVV D+SD D  +PKPGTLRHAVIQ  PLWI F   M IRL +E+I   +KT
Sbjct: 131 IGGKDGEFYVVTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDKT 190

Query: 254 LDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSDGDGI 313
           +D RG NVHI  G  +TLQ++KN+IIH +H+HDI  GNGG++RD+V H G RT+SDGDGI
Sbjct: 191 IDARGVNVHITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDGI 250

Query: 314 SLFGASKVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSDDRIM 373
           S+FGAS +WIDHVS+  C DGLIDA+  STGITISN HFT HN+V+L GASD  S D++M
Sbjct: 251 SIFGASYIWIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQVM 310

Query: 374 QVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFTAPPN 433
           Q+TLAFNHFGK L+QRMPRCRWG+ HVVNND+THW MYAIGGS +PTII QGNRF APP+
Sbjct: 311 QITLAFNHFGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPPD 370

Query: 434 PNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRGHSKKDV---ISAKPGT 493
              K+VTKR + P+SVW  W WRSEG+L +NGA+F +SG+P      KD+   ISA P  
Sbjct: 371 IFKKQVTKREYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDLYDGISAAPAE 430

Query: 494 YVTRLTRFSGTLHCRRNQPC 501
            VT +TRF+G L C+  +PC
Sbjct: 431 DVTWMTRFAGVLGCKPGKPC 449

BLAST of Sed0000677 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 4.1e-148
Identity = 249/438 (56.85%), Postives = 305/438 (69.63%), Query Frame = 0

Query: 82  MIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEVANNLNKEVNSALYGG---NKT 141
           ++ P ++ N+  FD  W +R  +A K    ++ P P  V N+ N  VN A+      N T
Sbjct: 22  IVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDT 81

Query: 142 RRNL---------GKYNGPCLATNPIDRCWRCDKNWYKNRKKLADCALGFGRGTIGGKDG 201
           RR L          K +G CLA NPID CWRCD+NW  NRKKLADC LGFGR T GGKDG
Sbjct: 82  RRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDG 141

Query: 202 EFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFTDNKTLDGRGA 261
             YVV+D+SD+D++NPKPGTLRHAV +D PLWI FA  M+I+L +EL+ T +KT+DGRGA
Sbjct: 142 PIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGA 201

Query: 262 NVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSDGDGISLFGAS 321
            V+I  G  +TLQFV N+IIHN++V  I  GNGGLIRDS +H G RT+SDGDGISLFGA+
Sbjct: 202 RVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGAT 261

Query: 322 KVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSDDRIMQVTLAF 381
            +WIDHVS+  C DG+IDAI  ST +TISN HFT H +V+L GA D +  D+ MQ+T+AF
Sbjct: 262 NIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAF 321

Query: 382 NHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFTAPPNPNCKEV 441
           NHFGK L QRMPRCR+G  HVVNND+THW MYAIGG+ NPTIISQGNRF APPN   K++
Sbjct: 322 NHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQI 381

Query: 442 TKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRGHSK-------KDVISAKPGTYV 501
           TKR + P   WKSWNW+SEGD  +NGA+FVQSG      SK       K  I  KPGT V
Sbjct: 382 TKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMV 441

BLAST of Sed0000677 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 7.8e-147
Identity = 244/465 (52.47%), Postives = 318/465 (68.39%), Query Frame = 0

Query: 73  KLLMIFAFVMIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEVANNLNK------ 132
           KL+  F   ++ PT++ NI E DE W +R+ EA++    A+  DP E+ ++ ++      
Sbjct: 8   KLMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNS 67

Query: 133 -EVNSALYGG-------------------NKTRRNL--------GKYNGPCLATNPIDRC 192
            +V +    G                   N TRR+L         K  GPC A+NPID+C
Sbjct: 68  TDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKC 127

Query: 193 WRCDKNWYKNRKKLADCALGFGRGTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDR 252
           WRC  +W K RKKL  C  GFG  T GGK G  YVV  + D DM+NPKPGTLRHAVIQ  
Sbjct: 128 WRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKE 187

Query: 253 PLWITFASDMVIRLVEELIFTDNKTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIK 312
           PLWI F +DM IRL +EL+   +KT+D RGANVH+AHG  +T+QFVKN+IIH +H+H I 
Sbjct: 188 PLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLHIHHIS 247

Query: 313 AGNGGLIRDSVSHYGFRTRSDGDGISLFGASKVWIDHVSLYHCTDGLIDAIMASTGITIS 372
             +GG+IRDSV H+G RTR+DGDG+S++G+S +W+DH+S+  C DGLIDAI+ STGITIS
Sbjct: 248 ESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITIS 307

Query: 373 NCHFTHHNDVLLLGASDGYSDDRIMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHW 432
           N HFTHHNDV+LLGA +    D+ MQVT+A+NHFGKGLVQRMPR RWGF HVVNND+THW
Sbjct: 308 NSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHW 367

Query: 433 RMYAIGGSSNPTIISQGNRFTAPPN-PNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAF 492
            +YAIGGS  PTI+S GNRF APP+ P+ +EVTKR +A +  WK WNWRS+ D+ +NGA+
Sbjct: 368 ELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAY 427

Query: 493 FVQSGNPVR--GHSKKDVISAKPGTYVTRLTRFSGTLHCRRNQPC 501
           F QSGNP     H+++ +I  K G  V++LT+++G L CR  + C
Sbjct: 428 FRQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

BLAST of Sed0000677 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 8.9e-143
Identity = 246/468 (52.56%), Postives = 314/468 (67.09%), Query Frame = 0

Query: 73  KLLMIFAFVMIFPTLKANIG-EFDEVWQERSKEAKKAAFDAFQPDPEEVANNLNKE--VN 132
           KL+ +     + PT++AN+  E DE W  ++ EA+K    A+ PDP E+ ++ ++    N
Sbjct: 8   KLVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDN 67

Query: 133 SALYGG----------------------NKTRRNL------------GKYNGPCLATNPI 192
           S    G                      N TRR+L             K  GPC A+NPI
Sbjct: 68  STDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPI 127

Query: 193 DRCWRCDKNWYKNRKKLADCALGFGRGTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVI 252
           D+CWRC  +W + RKKL  C  GFG  T GGK G  YVV    D DM+NP+PGTLRHAVI
Sbjct: 128 DKCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVI 187

Query: 253 QDRPLWITFASDMVIRLVEELIFTDNKTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVH 312
           Q  PLWI F  DM IRL +EL+ T +KT+D RGANVHIA+G  +T+Q+V NIIIH +HVH
Sbjct: 188 QKEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVH 247

Query: 313 DIKAGNGGLIRDSVSHYGFRTRSDGDGISLFGASKVWIDHVSLYHCTDGLIDAIMASTGI 372
            I   +GGLIRDS++H+G R  +DGDGIS+FGA+ +W+DH+S+  C DGLIDAIM ST I
Sbjct: 248 HIVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAI 307

Query: 373 TISNCHFTHHNDVLLLGASDGYSDDRIMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDF 432
           TISN HFTHHNDV+LLGA +   DD+ MQVT+A+NHFGKGLVQRMPR RWGF HVVNND+
Sbjct: 308 TISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDY 367

Query: 433 THWRMYAIGGSSNPTIISQGNRFTAPPN-PNCKEVTKRVFAPKSVWKSWNWRSEGDLTVN 492
           THW +YAIGGS  PTI+S GNRF APP+  + +EVTKR +A +S WK+WNWRSE D+ +N
Sbjct: 368 THWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMN 427

Query: 493 GAFFVQSGNP--VRGHSKKDVISAKPGTYVTRLTRFSGTLHCRRNQPC 501
            A+F QSGNP     HS++ +I  K G  V++LT+++G L CR  + C
Sbjct: 428 NAYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of Sed0000677 vs. ExPASy TrEMBL
Match: A0A0A0LR32 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G045510 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 5.5e-220
Identity = 353/439 (80.41%), Postives = 403/439 (91.80%), Query Frame = 0

Query: 62  MRETECSIFRIKLLMIFAFVMIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEVA 121
           MRET+ +I  IKLLMIF FVMIFPTLKANI +FDEVWQ+R+ EA+KA+F+A++P PEE  
Sbjct: 1   MRETKTTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAIEAQKASFEAYEPHPEEET 60

Query: 122 NNLNKEVNSALYGGNKTRRNLGKYNGPCLATNPIDRCWRCDKNWYKNRKKLADCALGFGR 181
           NN NK+V+ +L GGN TRR+L KY GPCLATNPIDRCWRCD+NW +NRKKLADCALGFGR
Sbjct: 61  NNFNKQVHRSLDGGNNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGR 120

Query: 182 GTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFTDN 241
            T GGKDG+ YVVRDSSD+D++NPKPGTLRHAVIQ+RPLWI FA DMVIRL EELI TD+
Sbjct: 121 RTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIVTDD 180

Query: 242 KTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSDGD 301
           KTLDGRGANVHIA+GGQ+TLQFVKNIIIHN+H+HDIKAGNGG+IRDSVSHYGFRTRSDGD
Sbjct: 181 KTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGMIRDSVSHYGFRTRSDGD 240

Query: 302 GISLFGASKVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSDDR 361
           GIS+FGAS+VWIDHVS+ +C DGLIDA+MAST ITISNCHFTHHNDV+LLGAS+GYS+D+
Sbjct: 241 GISMFGASRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASNGYSNDQ 300

Query: 362 IMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFTAP 421
           IMQVTLAFNHFGKGLVQRMPRCRWGF HVVNND+THW MYAIGGS NPTIISQGNRF AP
Sbjct: 301 IMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAP 360

Query: 422 PNPNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRGHSKKDVISAKPGTY 481
           PNPNCKEVTKRV+AP+S W+SWNWRSEGDL +NGAFF+QSGNP++ +SKKDVI +KPGT+
Sbjct: 361 PNPNCKEVTKRVYAPESEWRSWNWRSEGDLMMNGAFFIQSGNPIKRYSKKDVIHSKPGTF 420

Query: 482 VTRLTRFSGTLHCRRNQPC 501
           VTRLTRF+G L C++NQPC
Sbjct: 421 VTRLTRFAGPLKCKKNQPC 439

BLAST of Sed0000677 vs. ExPASy TrEMBL
Match: A0A5D3BH70 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003070 PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.5e-217
Identity = 350/439 (79.73%), Postives = 397/439 (90.43%), Query Frame = 0

Query: 62  MRETECSIFRIKLLMIFAFVMIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEVA 121
           MRET+ +I  IKLLMIF FVMIFPTLKANI +FDEVWQ+R+ EAKKA+ +A+QP PEEV 
Sbjct: 1   MRETKTTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAIEAKKASLEAYQPHPEEVT 60

Query: 122 NNLNKEVNSALYGGNKTRRNLGKYNGPCLATNPIDRCWRCDKNWYKNRKKLADCALGFGR 181
           NN NK+V+ +L G N TRR+L KY GPCLATNPIDRCWRCD+NW +NRKKLADCALGFGR
Sbjct: 61  NNFNKQVHRSLDGANNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGR 120

Query: 182 GTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFTDN 241
           GT GGKDG+ YVVRDSSD+D++NPKPGTLRHAVIQ+RPLWI FA DMVIRL EELI TDN
Sbjct: 121 GTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIMTDN 180

Query: 242 KTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSDGD 301
           KTLDGRGANVHIA+GGQ+TLQFV+NIIIHN+H+HDIKAGNGG IRDSVSHYG+RT+SDGD
Sbjct: 181 KTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGD 240

Query: 302 GISLFGASKVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSDDR 361
           GIS+FG+S+VWIDHVS+ +C DGLIDA+MAST ITISNCHFTHHNDV+LLGASD YS+D+
Sbjct: 241 GISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYSNDK 300

Query: 362 IMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFTAP 421
           IMQVTLAFNHFGKGLVQRMPRCRWGF HVVNND+THW MYAIGGS NPTIISQGNRF AP
Sbjct: 301 IMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAP 360

Query: 422 PNPNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRGHSKKDVISAKPGTY 481
           PNPN KEVTKRV+A +  W+SWNWRSEGDL +NGAFF+QSGNP+R +SKKDVI +KPGT+
Sbjct: 361 PNPNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRRYSKKDVIHSKPGTF 420

Query: 482 VTRLTRFSGTLHCRRNQPC 501
           VTRLTRF+G L C+ NQPC
Sbjct: 421 VTRLTRFAGPLKCKMNQPC 439

BLAST of Sed0000677 vs. ExPASy TrEMBL
Match: A0A6J1K214 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111489953 PE=3 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 2.2e-216
Identity = 354/440 (80.45%), Postives = 395/440 (89.77%), Query Frame = 0

Query: 62  MRETECSIFRIK-LLMIFAFVMIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEV 121
           MRE E +I   K LL+IFAF +IFPTL+A+I +FDEVWQ+R++EAKKAA +A+QP PEEV
Sbjct: 1   MRELESNIVGAKLLLLIFAFAIIFPTLEADIADFDEVWQQRAQEAKKAALEAYQPHPEEV 60

Query: 122 ANNLNKEVNSALYGGNKTRRNLGKYNGPCLATNPIDRCWRCDKNWYKNRKKLADCALGFG 181
             N N+ VN  L   N TRR+L KYNGPCLATNPIDRCWRCDKNW KNRKKLADCALGFG
Sbjct: 61  TENFNEHVNGDLDDANNTRRHLRKYNGPCLATNPIDRCWRCDKNWAKNRKKLADCALGFG 120

Query: 182 RGTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFTD 241
           RGT GGK+G FYVV+DSSD+DML+PKPGTLR+AVIQDRPLWITFA DMVIRL EELI TD
Sbjct: 121 RGTTGGKNGRFYVVQDSSDNDMLHPKPGTLRYAVIQDRPLWITFAHDMVIRLAEELIMTD 180

Query: 242 NKTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSDG 301
           NKTLDGRGANVHIA+GGQ+TLQFVKNIIIHN+H+HDIKAGNGGLIRDSV+HYG RTRSDG
Sbjct: 181 NKTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLIRDSVNHYGIRTRSDG 240

Query: 302 DGISLFGASKVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSDD 361
           DGIS+FGAS VWIDHVS+ +C DGLIDA+MAST ITISNCHF HHNDV+LLGASD +SDD
Sbjct: 241 DGISIFGASNVWIDHVSMSNCQDGLIDAVMASTAITISNCHFAHHNDVILLGASDSHSDD 300

Query: 362 RIMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFTA 421
           +IMQVTLAFNHFGKGLVQRMPRCRWGF HVVNND+THW MYAIGGS NPTIISQGNRF A
Sbjct: 301 QIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSRNPTIISQGNRFIA 360

Query: 422 PPNPNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRGHSKKDVISAKPGT 481
           PP+PNCKEVTKRV+AP+S WK+WNWRSEGDL +NGAFFV+SGNP+R +SKKDVI AKPGT
Sbjct: 361 PPDPNCKEVTKRVYAPESDWKTWNWRSEGDLLMNGAFFVESGNPIRKYSKKDVIHAKPGT 420

Query: 482 YVTRLTRFSGTLHCRRNQPC 501
           +VTRLTRF+G L C RNQPC
Sbjct: 421 FVTRLTRFAGPLKCTRNQPC 440

BLAST of Sed0000677 vs. ExPASy TrEMBL
Match: A0A6J1GTX4 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111457080 PE=3 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 3.4e-214
Identity = 349/441 (79.14%), Postives = 395/441 (89.57%), Query Frame = 0

Query: 62  MRETECSI--FRIKLLMIFAFVMIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEE 121
           MRE + +I   ++ LL+IFAF +IFP+L+A I +FDEVWQ+R++EAKKAA +A+QP PEE
Sbjct: 1   MRELDSNIAGAKLLLLLIFAFAIIFPSLEAGIADFDEVWQQRAQEAKKAALEAYQPRPEE 60

Query: 122 VANNLNKEVNSALYGGNKTRRNLGKYNGPCLATNPIDRCWRCDKNWYKNRKKLADCALGF 181
           V  + N+ VN  L   N TRR+L KYNGPCLATNPIDRCWRCDKNW KNRKKLADCALGF
Sbjct: 61  VTESFNEHVNGDLDDANNTRRHLRKYNGPCLATNPIDRCWRCDKNWSKNRKKLADCALGF 120

Query: 182 GRGTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFT 241
           GRGT GGK+G FY+V+DSSD+DML+PKPGTLR+AVIQDRPLWITFA DMVIRL EELI T
Sbjct: 121 GRGTTGGKNGRFYIVQDSSDNDMLHPKPGTLRYAVIQDRPLWITFAHDMVIRLAEELIMT 180

Query: 242 DNKTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSD 301
           DNKTLDGRGANVHIA+GGQ+TLQFVKNIIIHN+H+HDIKAGNGGLIRDSV+HYG RTRSD
Sbjct: 181 DNKTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLIRDSVNHYGIRTRSD 240

Query: 302 GDGISLFGASKVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSD 361
           GDGIS+FGAS VWIDHVS+ +C DGLIDA+MAST ITISNCHF HHNDV+LLGASD +SD
Sbjct: 241 GDGISMFGASNVWIDHVSMSNCQDGLIDAVMASTAITISNCHFAHHNDVILLGASDSHSD 300

Query: 362 DRIMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFT 421
           D+IMQVTLAFNHFGKGLVQRMPRCRWGF HVVNND+THW MYAIGGS NPTIISQGNRF 
Sbjct: 301 DQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSRNPTIISQGNRFI 360

Query: 422 APPNPNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRGHSKKDVISAKPG 481
           APP+PNCKEVTKRV+AP+S WK+WNWRSEGDL +NGAFFVQSGNP+R +SKKDVI AKPG
Sbjct: 361 APPDPNCKEVTKRVYAPESEWKTWNWRSEGDLLMNGAFFVQSGNPIRKYSKKDVIHAKPG 420

Query: 482 TYVTRLTRFSGTLHCRRNQPC 501
           T+VTRLTRF+G L C RN+PC
Sbjct: 421 TFVTRLTRFAGPLKCIRNKPC 441

BLAST of Sed0000677 vs. ExPASy TrEMBL
Match: A0A6J1C763 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009024 PE=3 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 7.0e-199
Identity = 320/408 (78.43%), Postives = 369/408 (90.44%), Query Frame = 0

Query: 94  FDEVWQERSKEAKKAAFDAFQPDPEEVANNLNKEVNSALYGGNKTRRNLGKYN-GPCLAT 153
           FDEVWQ+R++EAKKAA +A+QP PEEV +N N++V+ +L G N TRR+L  Y+ GPCLAT
Sbjct: 51  FDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLAT 110

Query: 154 NPIDRCWRCDKNWYKNRKKLADCALGFGRGTIGGKDGEFYVVRDSSDHDMLNPKPGTLRH 213
           NPIDRCWRCDK+W KNRKKLA+CALGFGRGT GGKDG +YVVRDSSD+D+LNPKPGTLR+
Sbjct: 111 NPIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLRY 170

Query: 214 AVIQDRPLWITFASDMVIRLVEELIFTDNKTLDGRGANVHIAHGGQMTLQFVKNIIIHNV 273
            VIQ++PLWI FA DMVIRL EELI TDNKT+DGRGANVHIA+GGQ+TLQFVKNIII N+
Sbjct: 171 GVIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISNL 230

Query: 274 HVHDIKAGNGGLIRDSVSHYGFRTRSDGDGISLFGASKVWIDHVSLYHCTDGLIDAIMAS 333
           H+HDIK G+GGLIRDSVSHYG RT SDGDGIS+FGA+K+WIDHVS+ +C DGLIDA+MAS
Sbjct: 231 HIHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMAS 290

Query: 334 TGITISNCHFTHHNDVLLLGASDGYSDDRIMQVTLAFNHFGKGLVQRMPRCRWGFFHVVN 393
           TGITISNCHFT HNDV+LLGASD YS+D+IMQVTLAFNHFGKGLVQRMPRCRWGF HVVN
Sbjct: 291 TGITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVN 350

Query: 394 NDFTHWRMYAIGGSSNPTIISQGNRFTAPPNPNCKEVTKRVFAPKSVWKSWNWRSEGDLT 453
           ND+THW MYAIGGS NPTIISQGNRF APP+PNCKEVTKR +A +S WKSWNWRSEGDL 
Sbjct: 351 NDYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDLM 410

Query: 454 VNGAFFVQSGNPVRGHSKKDVISAKPGTYVTRLTRFSGTLHCRRNQPC 501
           +NGAFF++SG+P+R +SKKDVI+AKPG++VTRLTRFSG L CR N+PC
Sbjct: 411 MNGAFFIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 458

BLAST of Sed0000677 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 526.2 bits (1354), Expect = 2.9e-149
Identity = 249/438 (56.85%), Postives = 305/438 (69.63%), Query Frame = 0

Query: 82  MIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEVANNLNKEVNSALYGG---NKT 141
           ++ P ++ N+  FD  W +R  +A K    ++ P P  V N+ N  VN A+      N T
Sbjct: 22  IVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDT 81

Query: 142 RRNL---------GKYNGPCLATNPIDRCWRCDKNWYKNRKKLADCALGFGRGTIGGKDG 201
           RR L          K +G CLA NPID CWRCD+NW  NRKKLADC LGFGR T GGKDG
Sbjct: 82  RRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDG 141

Query: 202 EFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFTDNKTLDGRGA 261
             YVV+D+SD+D++NPKPGTLRHAV +D PLWI FA  M+I+L +EL+ T +KT+DGRGA
Sbjct: 142 PIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGA 201

Query: 262 NVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSDGDGISLFGAS 321
            V+I  G  +TLQFV N+IIHN++V  I  GNGGLIRDS +H G RT+SDGDGISLFGA+
Sbjct: 202 RVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGAT 261

Query: 322 KVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSDDRIMQVTLAF 381
            +WIDHVS+  C DG+IDAI  ST +TISN HFT H +V+L GA D +  D+ MQ+T+AF
Sbjct: 262 NIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAF 321

Query: 382 NHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFTAPPNPNCKEV 441
           NHFGK L QRMPRCR+G  HVVNND+THW MYAIGG+ NPTIISQGNRF APPN   K++
Sbjct: 322 NHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQI 381

Query: 442 TKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRGHSK-------KDVISAKPGTYV 501
           TKR + P   WKSWNW+SEGD  +NGA+FVQSG      SK       K  I  KPGT V
Sbjct: 382 TKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMV 441

BLAST of Sed0000677 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 521.5 bits (1342), Expect = 7.2e-148
Identity = 243/465 (52.26%), Postives = 318/465 (68.39%), Query Frame = 0

Query: 73  KLLMIFAFVMIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEVANNLNK------ 132
           KL+  F   ++ PT++ NI E DE W +R+ EA++    A+  DP E+ ++ ++      
Sbjct: 8   KLMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNS 67

Query: 133 -EVNSALYGG-------------------NKTRRNL--------GKYNGPCLATNPIDRC 192
            +V +    G                   N TRR+L         K  GPC A+NPID+C
Sbjct: 68  TDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKC 127

Query: 193 WRCDKNWYKNRKKLADCALGFGRGTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDR 252
           WRC  +W K RKKL  C  GFG  T GGK G  YVV  + D DM+NPKPGTLRHAVIQ  
Sbjct: 128 WRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKE 187

Query: 253 PLWITFASDMVIRLVEELIFTDNKTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIK 312
           PLWI F +DM IRL +EL+   +KT+D RGANVH+AHG  +T+QFVKN++IH +H+H I 
Sbjct: 188 PLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIHHIS 247

Query: 313 AGNGGLIRDSVSHYGFRTRSDGDGISLFGASKVWIDHVSLYHCTDGLIDAIMASTGITIS 372
             +GG+IRDSV H+G RTR+DGDG+S++G+S +W+DH+S+  C DGLIDAI+ STGITIS
Sbjct: 248 ESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITIS 307

Query: 373 NCHFTHHNDVLLLGASDGYSDDRIMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHW 432
           N HFTHHNDV+LLGA +    D+ MQVT+A+NHFGKGLVQRMPR RWGF HVVNND+THW
Sbjct: 308 NSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHW 367

Query: 433 RMYAIGGSSNPTIISQGNRFTAPPN-PNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAF 492
            +YAIGGS  PTI+S GNRF APP+ P+ +EVTKR +A +  WK WNWRS+ D+ +NGA+
Sbjct: 368 ELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAY 427

Query: 493 FVQSGNPVR--GHSKKDVISAKPGTYVTRLTRFSGTLHCRRNQPC 501
           F QSGNP     H+++ +I  K G  V++LT+++G L CR  + C
Sbjct: 428 FRQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

BLAST of Sed0000677 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 508.4 bits (1308), Expect = 6.3e-144
Identity = 246/468 (52.56%), Postives = 314/468 (67.09%), Query Frame = 0

Query: 73  KLLMIFAFVMIFPTLKANIG-EFDEVWQERSKEAKKAAFDAFQPDPEEVANNLNKE--VN 132
           KL+ +     + PT++AN+  E DE W  ++ EA+K    A+ PDP E+ ++ ++    N
Sbjct: 8   KLVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDN 67

Query: 133 SALYGG----------------------NKTRRNL------------GKYNGPCLATNPI 192
           S    G                      N TRR+L             K  GPC A+NPI
Sbjct: 68  STDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPI 127

Query: 193 DRCWRCDKNWYKNRKKLADCALGFGRGTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVI 252
           D+CWRC  +W + RKKL  C  GFG  T GGK G  YVV    D DM+NP+PGTLRHAVI
Sbjct: 128 DKCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVI 187

Query: 253 QDRPLWITFASDMVIRLVEELIFTDNKTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVH 312
           Q  PLWI F  DM IRL +EL+ T +KT+D RGANVHIA+G  +T+Q+V NIIIH +HVH
Sbjct: 188 QKEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVH 247

Query: 313 DIKAGNGGLIRDSVSHYGFRTRSDGDGISLFGASKVWIDHVSLYHCTDGLIDAIMASTGI 372
            I   +GGLIRDS++H+G R  +DGDGIS+FGA+ +W+DH+S+  C DGLIDAIM ST I
Sbjct: 248 HIVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAI 307

Query: 373 TISNCHFTHHNDVLLLGASDGYSDDRIMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDF 432
           TISN HFTHHNDV+LLGA +   DD+ MQVT+A+NHFGKGLVQRMPR RWGF HVVNND+
Sbjct: 308 TISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDY 367

Query: 433 THWRMYAIGGSSNPTIISQGNRFTAPPN-PNCKEVTKRVFAPKSVWKSWNWRSEGDLTVN 492
           THW +YAIGGS  PTI+S GNRF APP+  + +EVTKR +A +S WK+WNWRSE D+ +N
Sbjct: 368 THWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMN 427

Query: 493 GAFFVQSGNP--VRGHSKKDVISAKPGTYVTRLTRFSGTLHCRRNQPC 501
            A+F QSGNP     HS++ +I  K G  V++LT+++G L CR  + C
Sbjct: 428 NAYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of Sed0000677 vs. TAIR 10
Match: AT1G04680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.3e-141
Identity = 231/386 (59.84%), Postives = 284/386 (73.58%), Query Frame = 0

Query: 116 DPEEVANNLNKEVNSALYGGNKTRRNLGKYNGPCLATNPIDRCWRCDKNWYKNRKKLADC 175
           +P+EVA+ +   +       +  RR LG +   C   NPID CWRCD NW+KNRK+LADC
Sbjct: 47  NPDEVADEV-LALTEMSVRNHTERRKLGYFT--CGTGNPIDDCWRCDPNWHKNRKRLADC 106

Query: 176 ALGFGRGTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEE 235
            +GFGR  IGG+DG FYVV D  D + +NP+PGTLRHAVIQDRPLWI F  DMVI+L +E
Sbjct: 107 GIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRPLWIVFKRDMVIQLKQE 166

Query: 236 LIFTDNKTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFR 295
           LI    KT+DGRGANVHIA+GG +T+QFV N+I+H +H+HD K     ++R S +H+G+R
Sbjct: 167 LIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKPTGNAMVRSSETHFGWR 226

Query: 296 TRSDGDGISLFGASKVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASD 355
           T +DGD IS+FG+S VWIDH SL HC DGL+DA+M ST ITISN H THHN+V+LLG SD
Sbjct: 227 TMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISNNHLTHHNEVMLLGHSD 286

Query: 356 GYSDDRIMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQG 415
            Y  D+ MQVT+A+NHFG GL+QRMPRCR G+FHVVNND+THW MYAIGGS+NPTI SQG
Sbjct: 287 SYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 346

Query: 416 NRFTAPPNPNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSGNPVRG-HSKKDVI 475
           NR+ AP NP  KEVTKRV  P S WK WNWRSEGDL  NGA+F  SG    G +++   +
Sbjct: 347 NRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGSYARASSL 406

Query: 476 SAKPGTYVTRLTRFSGTLHCRRNQPC 501
           SAK  + V  +T  +G L CRR + C
Sbjct: 407 SAKSSSLVGHITSDAGALPCRRGRQC 429

BLAST of Sed0000677 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 499.2 bits (1284), Expect = 3.8e-141
Identity = 242/448 (54.02%), Postives = 312/448 (69.64%), Query Frame = 0

Query: 77  IFAFV-----MIFPTLKANIGEFDEVWQERSKEAKKAAFDAFQPDPEEVANNLNKEVNSA 136
           +F FV     ++ P+++A+I E+DE W +R   A +   +++ P+PE V ++ N     A
Sbjct: 8   VFVFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYHAALA 67

Query: 137 LYGG---NKTRRNL---------GKYNGPCLATNPIDRCWRCDKNWYKNRKKLADCALGF 196
           +      N+TRR+L          +  G   + N ID+CWR DKNW KNRKKLADC LGF
Sbjct: 68  METTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVLGF 127

Query: 197 GRGTIGGKDGEFYVVRDSSDHDMLNPKPGTLRHAVIQDRPLWITFASDMVIRLVEELIFT 256
           GR T GGK+G  YVV D SD+D+L PKPGT+RHAV +DRPLWI FA  M+I+L +ELI T
Sbjct: 128 GRKTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELIIT 187

Query: 257 DNKTLDGRGANVHIAHGGQMTLQFVKNIIIHNVHVHDIKAGNGGLIRDSVSHYGFRTRSD 316
           ++KT+DGRGA ++I  G  +TLQFV+N+IIHN+H+  IK G GGLI DS  H+G RT SD
Sbjct: 188 NDKTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTVSD 247

Query: 317 GDGISLFGASKVWIDHVSLYHCTDGLIDAIMASTGITISNCHFTHHNDVLLLGASDGYSD 376
           GDGI++FGA+ VWIDHVS+  C+DG+IDAIM ST ITISN HFT H++V+L G ++    
Sbjct: 248 GDGINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKDVI 307

Query: 377 DRIMQVTLAFNHFGKGLVQRMPRCRWGFFHVVNNDFTHWRMYAIGGSSNPTIISQGNRFT 436
           D+ MQ+T+AFNHFGK L QRMPR R+G  HVVNND+THW MYAIGG+ NPTIISQGNRF 
Sbjct: 308 DKKMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNRFI 367

Query: 437 APPNPNCKEVTKRVFAPKSVWKSWNWRSEGDLTVNGAFFVQSG-------NPVRGHSKKD 496
           APP  + K+VTKR + P   WKSWNW+SE D  +NGA+FVQSG        P     +K 
Sbjct: 368 APPIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKF 427

Query: 497 VISAKPGTYVTRLTRFSGTLHCRRNQPC 501
            I  +PGT V RLT+ +GTL C+  + C
Sbjct: 428 AIRPQPGTKVRRLTKDAGTLGCKPGKSC 455