Sed0000489 (gene) Chayote v1

Overview
NameSed0000489
Typegene
OrganismSechium edule (Chayote v1)
Descriptionprotein NRT1/ PTR FAMILY 5.2-like
LocationLG02: 50500512 .. 50504523 (-)
RNA-Seq ExpressionSed0000489
SyntenySed0000489
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTACAGCAACAGATCGAGAAACCGGGCACAACGACTACACTAAAGATGGAACCGTGGATCTTAAAGGCAACCCCGTTCTTCGCTCCAAAACCGGCCATTGGAAAGCCTGTTCTTTCATCATCGGTAAGTTTAAAAAATTCGTAGGAAATTAATTACATGCGATTGACGTGAGAATAATTGTGCTTGGATTAGTATCTTACAGGGTATGAGCTAATTGAAAGGATGATGTTCAATGGGATTTCAGTGAATCTCATGATATATTTGACCACCAAACTCAATCAAGGCACTGTCACAGCCTCTAACAATGTCACCAATTGGACTGGAACAGTTTGGATGATGCCCGTCTTGGGAGCTTACATTGCCGACGCTCATCTTGGCCGATATCGAACCTTATTGATCTCCTCTATCATCTGCCTTGTGGTATTGTTCCTGCTAATTTGTGTTTTGTTTTTAGTTCAATGAAGATTCAAACTTGTCTTTCAATAAATATTATGACCAAGCCTAAGATGTCTAAGTTACAATTAACAAAAATAATTAAAATCATAACAGCTTCTAAGCTTATATTTAATTAGTATTTTTTTTTTTAAAATAAATAAATTGAATTCTTTTGGTTAAACAAGTGTAAGAATCGAATCCATAATAGTTAGGGATAAACACTTGTTTTCACTCTTGCAAACTTTGATTTGGGAGTTATTTTTTCGTTTTTGAAAAAATTGAATTTTTTTTATTGGAGAAAAAGTCAACAAACGTAGTATGTCCAATTGGGACCAACTAGGATAAAAATAGATTTTTTTAAAAAACTAGGATAAAAATAAACCATTCGTGTTAATGTAACAAGTGTGTCTTTTCAAAAAAAAAAAACAAGTGGGCACGTGCCGCGCCTTATTTATTTGCTTTTAAGTTTTTTTAATCACTTAACTTAAAACTCTTGCATCTCATTAAAATTTAAACTATGATTGCATATCATACCAAGAGAGACGGTGATTTGATGGTTTTATTGTTTGGGGGTAATTCAATTCAACATCATAATTATAGGTTCGATTTCAAAAATTGATATTTGGTTCGATCTGTTGATAGTGATATATATCTCTTAACTAAGAAATCGTAAATGGTGAATCTTCAACCTTCTTAAGTACTAAAAAAAATCATACAAAAACAAAAATCTACTAAATATTTTAAATTTCAAATGAAGCTTTACAATTCATAAGTTGAATAAAGACAGATTTGAACATTATTATGAATTACTTTAGCACAGGCAATGTCGGTTTTAACACTAGCAGTATCAATCCCAAGCCTTAAACCACCTCCATGTCAAGAAGCCAACAAAGATAACTGCAAACAACAGGCTACTAAATTACAGCTAGCCGTGTTCTTCGGCTCGCTATACTTGTTGGCAGTTGCCTCGGGCGGTACGAAACCGAACATCTCGACGTTGGGAGCGGACCAGTTCGACGATTTCGATCCGAAAGAGAAGGCTCAAAAGCTGTCCTTCTTCAACTGGTGGTTGTTCACTGTCTTCTCTGGCATTCTGTTTGCCTCCACCATTTTGGTTTACATTCAAGACAATGTGGGTTGGGGCTTAGGATATGGCATTCCAACTATTGGTGTTGCATTTGCCATTCTTATATTTGTTTGTGGCACACCCTTTTATAGGCATAAATTAATTTCCTATAAAGGGGGCCCTTTTATTAGAATGGCTAATGTCATTGTTGCTGCTGCTTGGAATTGGAGCCTCCCTCTTCCTAATGACCCAAAGGAACTTTATGAGCTTGAGATTCAACACTACTCCAAGAGTACAACTTTCAAGATTGATACCACCCCATCCTTGAGGTGAGTTTCTATTACAGTTCTTGAACTTTCGATTTTGTATAACTCTTCGTGTGTTACGGAAGCATTAAATTTGTTGGGACATAAACAAATATCGACGAACAAAGAATAAAAGAATGAAAAGATCAATTCATAGATTTACATAGTTCGTACACTGTGTCCTAGTTACGTCCACATGCTTGTATATATATTTGCATCCCTCTTAAACGATACACTCAATGTACGCTACAATAAAATCCATCAACAGTGAGTGAAGTTGTGTTTGGAGTAACAACTGAATTATTATAAACGGTGGGTTATTATAAATTATGTTTAGGGTACAAATTATAACGAGGGTTATGAAAACAAGGTTTGAATGTAAGTTATTAGAACAAGGAAGAGAAAATGGTTAATCTCAAAGAGGACAAGAATTATATGAAATAGTGATAGAAATTATATTAGATGGTGGCTTGGGTTTGGGCTAAACGTGGATTGAGTTATCATAACTTAACCTCACGCGTGAAGTACCTAACTGATTCAAGTCATTACAACAAAAATAGTAATAACATGTCTCTTTTTTGGAAACTGGAAACGAACTTCGTTCCCAATATCCGGGGTCCGATACCGCATTTTTGAAGTATATGGACTCTATTGACAGTTTTGGAAATAAAGTTGCAATTTAGTTGCAGTTAGATTATTGTTCTCATCACGTGTTCTGTTTGCCTACTCTGCTTCTGTGTACAATCAGTGATTGAATTTGTGTAAGTAATAATGCATGGCATTATATGTAGGTTTCTGAACAAGGCTGCTATAAGAACTAATGCAAGTAATTCATGGAGGTTATGCTCAGTGACCCAAGTGGAGGAGACAAAACAAATGCTGAGAATGATCCCAATTCTGATATGCACATTCATGCCTAGCACAATGGTGGCACAAACACACACCCTTTTCATCAAACAAGGAACCACTCTCGATCGAAGCATCGGCAGCCACTTCGAAGTCCCCCCTGCAAGTTTGTATGCTTTCATTACCATCTCCATGCTTGTCTCCATTGTCATCTACGACAGGTTTGCCCAATTCTGTCTCTAATTATGGTTTTAAAACATTATCTTCTTAAGTATTGCTCGTTTACCTTTTTTGTGTTTCTTTGCACTCCAAGTGGAAGGATTCAAACATTCGACTTATTAGTTGAGAATATATACGTTAACCAGTTGAACTATGTTAAGATTATTAAAGGGTTTTTGAGGCAAGGAAAATATTCCCACGTTTGGGGCACTAACAGTCGCTCCCAGAATCAATATTCAATCCCTGAGCTAACGGTTCCCTCATATTCCTTTTTTCACCTCAAACACGATTAATCATTCTCTCATGTATATCCATTCCATCATAATCACTTTCATTCTCCCTACTCCAAACGTCCTCTAAATGATTTTCTTTATCTATGGAATTTGTAGTAGAGTTCGAAAAATAGAGACCAATATGATCATGTTCTTTAAAAGCACATAGGTGCAAAGGGATGCTTTGAAAGTAGCTAGAAAGTCAAAATATCCTATCTAAACCTGGTTAATACATTTATATTTTCATTTTTGTTACAGGATGTTTGTGAAGATAATGCAAAAAGTGACCAAAAACCCAAGAGGAATCACAATGGTACAAAGAATGGGACTTGGACTGATCTGTCATGTGCTGGTAATGACAGTGGCTTCTAGAGTTGAAAGGCACAGACTTGAAGTTGCGAGAACAAATGGGTTGGTGCAAAATGGATCAGGACATGAACAACTTCCCTTAACCATTTTTACTTTGCTCCCTCAGTTCATTCTAATGGGGATTGCTGATGCACTGCTTCAAATAGCCAACACTGAATTCTTCTATGATCAAGCCCCAGAAAGCATGAAGAGTTTAGGCAGTTCTTACACGTTGACTTCGCTCGGAATCGGGAACTTCCTCAGCAGTTTTATACTATCAACAGTTTCTAACATTACCAAAAAACATGGGAATGGTTGGATTCTGAACAACTTGAATGCTTCTCATCTTGATTACTTCTATGCCTTACTTGCTGTCATGAGTGCTGTTAACTTCTTCCTGTTTTTGGTTGTATCCCATTTCTATGAGTACAAAGCTGAAGTCTCCGACTCGATCGAAGTTCTCACCGATGAACTCAAGAATAAGAATTTCAAAGGCGTCCAACCACCCGGATAA

mRNA sequence

ATGGCGGCTACAGCAACAGATCGAGAAACCGGGCACAACGACTACACTAAAGATGGAACCGTGGATCTTAAAGGCAACCCCGTTCTTCGCTCCAAAACCGGCCATTGGAAAGCCTGTTCTTTCATCATCGGGTATGAGCTAATTGAAAGGATGATGTTCAATGGGATTTCAGTGAATCTCATGATATATTTGACCACCAAACTCAATCAAGGCACTGTCACAGCCTCTAACAATGTCACCAATTGGACTGGAACAGTTTGGATGATGCCCGTCTTGGGAGCTTACATTGCCGACGCTCATCTTGGCCGATATCGAACCTTATTGATCTCCTCTATCATCTGCCTTGTGGCAATGTCGGTTTTAACACTAGCAGTATCAATCCCAAGCCTTAAACCACCTCCATGTCAAGAAGCCAACAAAGATAACTGCAAACAACAGGCTACTAAATTACAGCTAGCCGTGTTCTTCGGCTCGCTATACTTGTTGGCAGTTGCCTCGGGCGGTACGAAACCGAACATCTCGACGTTGGGAGCGGACCAGTTCGACGATTTCGATCCGAAAGAGAAGGCTCAAAAGCTGTCCTTCTTCAACTGGTGGTTGTTCACTGTCTTCTCTGGCATTCTGTTTGCCTCCACCATTTTGGTTTACATTCAAGACAATGTGGGTTGGGGCTTAGGATATGGCATTCCAACTATTGGTGTTGCATTTGCCATTCTTATATTTGTTTGTGGCACACCCTTTTATAGGCATAAATTAATTTCCTATAAAGGGGGCCCTTTTATTAGAATGGCTAATGTCATTGTTGCTGCTGCTTGGAATTGGAGCCTCCCTCTTCCTAATGACCCAAAGGAACTTTATGAGCTTGAGATTCAACACTACTCCAAGAGTACAACTTTCAAGATTGATACCACCCCATCCTTGAGGTTTCTGAACAAGGCTGCTATAAGAACTAATGCAAGTAATTCATGGAGGTTATGCTCAGTGACCCAAGTGGAGGAGACAAAACAAATGCTGAGAATGATCCCAATTCTGATATGCACATTCATGCCTAGCACAATGGTGGCACAAACACACACCCTTTTCATCAAACAAGGAACCACTCTCGATCGAAGCATCGGCAGCCACTTCGAAGTCCCCCCTGCAAGTTTGTATGCTTTCATTACCATCTCCATGCTTGTCTCCATTGTCATCTACGACAGGATGTTTGTGAAGATAATGCAAAAAGTGACCAAAAACCCAAGAGGAATCACAATGGTACAAAGAATGGGACTTGGACTGATCTGTCATGTGCTGGTAATGACAGTGGCTTCTAGAGTTGAAAGGCACAGACTTGAAGTTGCGAGAACAAATGGGTTGGTGCAAAATGGATCAGGACATGAACAACTTCCCTTAACCATTTTTACTTTGCTCCCTCAGTTCATTCTAATGGGGATTGCTGATGCACTGCTTCAAATAGCCAACACTGAATTCTTCTATGATCAAGCCCCAGAAAGCATGAAGAGTTTAGGCAGTTCTTACACGTTGACTTCGCTCGGAATCGGGAACTTCCTCAGCAGTTTTATACTATCAACAGTTTCTAACATTACCAAAAAACATGGGAATGGTTGGATTCTGAACAACTTGAATGCTTCTCATCTTGATTACTTCTATGCCTTACTTGCTGTCATGAGTGCTGTTAACTTCTTCCTGTTTTTGGTTGTATCCCATTTCTATGAGTACAAAGCTGAAGTCTCCGACTCGATCGAAGTTCTCACCGATGAACTCAAGAATAAGAATTTCAAAGGCGTCCAACCACCCGGATAA

Coding sequence (CDS)

ATGGCGGCTACAGCAACAGATCGAGAAACCGGGCACAACGACTACACTAAAGATGGAACCGTGGATCTTAAAGGCAACCCCGTTCTTCGCTCCAAAACCGGCCATTGGAAAGCCTGTTCTTTCATCATCGGGTATGAGCTAATTGAAAGGATGATGTTCAATGGGATTTCAGTGAATCTCATGATATATTTGACCACCAAACTCAATCAAGGCACTGTCACAGCCTCTAACAATGTCACCAATTGGACTGGAACAGTTTGGATGATGCCCGTCTTGGGAGCTTACATTGCCGACGCTCATCTTGGCCGATATCGAACCTTATTGATCTCCTCTATCATCTGCCTTGTGGCAATGTCGGTTTTAACACTAGCAGTATCAATCCCAAGCCTTAAACCACCTCCATGTCAAGAAGCCAACAAAGATAACTGCAAACAACAGGCTACTAAATTACAGCTAGCCGTGTTCTTCGGCTCGCTATACTTGTTGGCAGTTGCCTCGGGCGGTACGAAACCGAACATCTCGACGTTGGGAGCGGACCAGTTCGACGATTTCGATCCGAAAGAGAAGGCTCAAAAGCTGTCCTTCTTCAACTGGTGGTTGTTCACTGTCTTCTCTGGCATTCTGTTTGCCTCCACCATTTTGGTTTACATTCAAGACAATGTGGGTTGGGGCTTAGGATATGGCATTCCAACTATTGGTGTTGCATTTGCCATTCTTATATTTGTTTGTGGCACACCCTTTTATAGGCATAAATTAATTTCCTATAAAGGGGGCCCTTTTATTAGAATGGCTAATGTCATTGTTGCTGCTGCTTGGAATTGGAGCCTCCCTCTTCCTAATGACCCAAAGGAACTTTATGAGCTTGAGATTCAACACTACTCCAAGAGTACAACTTTCAAGATTGATACCACCCCATCCTTGAGGTTTCTGAACAAGGCTGCTATAAGAACTAATGCAAGTAATTCATGGAGGTTATGCTCAGTGACCCAAGTGGAGGAGACAAAACAAATGCTGAGAATGATCCCAATTCTGATATGCACATTCATGCCTAGCACAATGGTGGCACAAACACACACCCTTTTCATCAAACAAGGAACCACTCTCGATCGAAGCATCGGCAGCCACTTCGAAGTCCCCCCTGCAAGTTTGTATGCTTTCATTACCATCTCCATGCTTGTCTCCATTGTCATCTACGACAGGATGTTTGTGAAGATAATGCAAAAAGTGACCAAAAACCCAAGAGGAATCACAATGGTACAAAGAATGGGACTTGGACTGATCTGTCATGTGCTGGTAATGACAGTGGCTTCTAGAGTTGAAAGGCACAGACTTGAAGTTGCGAGAACAAATGGGTTGGTGCAAAATGGATCAGGACATGAACAACTTCCCTTAACCATTTTTACTTTGCTCCCTCAGTTCATTCTAATGGGGATTGCTGATGCACTGCTTCAAATAGCCAACACTGAATTCTTCTATGATCAAGCCCCAGAAAGCATGAAGAGTTTAGGCAGTTCTTACACGTTGACTTCGCTCGGAATCGGGAACTTCCTCAGCAGTTTTATACTATCAACAGTTTCTAACATTACCAAAAAACATGGGAATGGTTGGATTCTGAACAACTTGAATGCTTCTCATCTTGATTACTTCTATGCCTTACTTGCTGTCATGAGTGCTGTTAACTTCTTCCTGTTTTTGGTTGTATCCCATTTCTATGAGTACAAAGCTGAAGTCTCCGACTCGATCGAAGTTCTCACCGATGAACTCAAGAATAAGAATTTCAAAGGCGTCCAACCACCCGGATAA

Protein sequence

MAATATDRETGHNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNLMIYLTTKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSVLTLAVSIPSLKPPPCQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILIFVCGTPFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFKIDTTPSLRFLNKAAIRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTLFIKQGTTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQRMGLGLICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIADALLQIANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGNGWILNNLNASHLDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEVLTDELKNKNFKGVQPPG
Homology
BLAST of Sed0000489 vs. NCBI nr
Match: XP_031737789.1 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Cucumis sativus] >KAE8650326.1 hypothetical protein Csa_011590 [Cucumis sativus])

HSP 1 Score: 951.0 bits (2457), Expect = 4.8e-273
Identity = 477/604 (78.97%), Postives = 541/604 (89.57%), Query Frame = 0

Query: 1   MAATATDRETGHND---YTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGIS 60
           MA  A D+E G  D   YTKDGTVD KGN VLRSKTG WKACSFI+GYELIERMMFNGI+
Sbjct: 1   MADAAADQEAGVLDVDNYTKDGTVDWKGNAVLRSKTGRWKACSFILGYELIERMMFNGIA 60

Query: 61  VNLMIYLTTKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVA 120
            NL+IYLTTKLNQGT+TASNNVTNWTGTVW+MP+LGAY+ADAHLGRYRT  ISS++C +A
Sbjct: 61  ANLIIYLTTKLNQGTLTASNNVTNWTGTVWIMPILGAYVADAHLGRYRTFFISSLVCFMA 120

Query: 121 MSVLTLAVSIPSLKPPPCQEA-NKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTL 180
           MS+LTL VSIPSLKPPPC  A +K+NCK QA+KLQLA+FFGSLYLLA+ASGGTKPNIST+
Sbjct: 121 MSLLTLTVSIPSLKPPPCSTAISKENCK-QASKLQLAMFFGSLYLLAIASGGTKPNISTM 180

Query: 181 GADQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAF 240
           GADQFDDFDPKEKAQKLSFFNWWLF+VFSGILFASTILVYIQDNVGW LGYGIPTIG+  
Sbjct: 181 GADQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGV 240

Query: 241 AILIFVCGTPFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKS 300
           AILIFV GTPFYRHKL S  G PFI+MANVIVAA WNW LPLPNDP +LYEL++QHYSK+
Sbjct: 241 AILIFVVGTPFYRHKLPS--GSPFIKMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKN 300

Query: 301 TTFKIDTTPSLRFLNKAAIRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQ 360
            TFKID+TPSLRFLNKAA+R  +S+ WR+C+VT+VEETKQM+RMIPI++CTF+PSTMVAQ
Sbjct: 301 GTFKIDSTPSLRFLNKAALRGVSSDPWRICTVTEVEETKQMVRMIPIMVCTFLPSTMVAQ 360

Query: 361 THTLFIKQGTTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGI 420
           +HTLFIKQGTTLDRSIGSHF+VPPASLYAF+TISML++I+IYDR+FVKIMQ+VTKNPRGI
Sbjct: 361 SHTLFIKQGTTLDRSIGSHFKVPPASLYAFVTISMLLTILIYDRIFVKIMQRVTKNPRGI 420

Query: 421 TMVQRMGLGLICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILM 480
           TM+QRMG+G+ICHVLVMTVAS+VE+HRL +A   G   +    ++LPLTIF LLPQFIL 
Sbjct: 421 TMLQRMGIGMICHVLVMTVASQVEKHRLHIAAKYG--SSAEVQKELPLTIFILLPQFILT 480

Query: 481 GIADALLQIANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGNGW 540
           G+ADA LQIA+ EFFYDQAPE+MKSLGSSY +TSLGIGNFLSSFILS VS ITK+ GNGW
Sbjct: 481 GVADAFLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSSFILSKVSEITKRQGNGW 540

Query: 541 ILNNLNASHLDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEVLTDELKNKNFKGV 600
           ILNNLNASHLDYFYALLAVMS+VN F+FL++S FY YKAEVSDSI+VLTDELK K  KG+
Sbjct: 541 ILNNLNASHLDYFYALLAVMSSVNLFIFLLISKFYIYKAEVSDSIQVLTDELKKKKSKGL 599

BLAST of Sed0000489 vs. NCBI nr
Match: XP_038874856.1 (protein NRT1/ PTR FAMILY 5.2-like [Benincasa hispida])

HSP 1 Score: 946.4 bits (2445), Expect = 1.2e-271
Identity = 479/605 (79.17%), Postives = 541/605 (89.42%), Query Frame = 0

Query: 1   MAATATDRETG--HNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISV 60
           MA  A D+ETG   ++YTKDGTVD KGNPVLRSKTG WKACSFII YELI+RMMFNGI+ 
Sbjct: 1   MAGAAADQETGLQLDNYTKDGTVDRKGNPVLRSKTGRWKACSFIIVYELIDRMMFNGIAA 60

Query: 61  NLMIYLTTKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAM 120
           NL+IYLTTKLNQGT+TASNNVTNWTGTVW+ P+LGAY+ADAHLGRYRT  ISS++CL+AM
Sbjct: 61  NLIIYLTTKLNQGTLTASNNVTNWTGTVWITPILGAYVADAHLGRYRTFFISSLLCLIAM 120

Query: 121 SVLTLAVSIPSLKPPPCQEA-NKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLG 180
           S+LTLAVS+PSLKPPPC EA +K+NCK QA+KLQLAVFFGSLYLLA+ASGGTKPNIST+G
Sbjct: 121 SLLTLAVSVPSLKPPPCLEAISKENCK-QASKLQLAVFFGSLYLLALASGGTKPNISTMG 180

Query: 181 ADQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFA 240
           ADQFDDFDPKEKAQKLSFFNWWLF+VFSGILFASTILVYIQDNVGW LGYGIP IG+  A
Sbjct: 181 ADQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPPIGLGVA 240

Query: 241 ILIFVCGTPFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKST 300
           ILIFV GTPFYRH+L +  G PF  MANVI+AA WNW  PLPNDP ELYELEIQHYSK  
Sbjct: 241 ILIFVVGTPFYRHRLPN--GSPFTTMANVIIAAIWNWRFPLPNDPNELYELEIQHYSKHG 300

Query: 301 TFKIDTTPSLRFLNKAAIRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQT 360
           TFKID+TPSLRFLNKAAIR ++S+ WR+C+VT+VEETKQM+RMIPI+ICTF+P+TMVAQ+
Sbjct: 301 TFKIDSTPSLRFLNKAAIRRDSSDPWRMCTVTEVEETKQMVRMIPIMICTFIPNTMVAQS 360

Query: 361 HTLFIKQGTTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGIT 420
           HTLFIKQGTTLDRSIGSHF+VPPASLY+F+TISML+SI+IYDR+FVKIMQ+VTKNPRGIT
Sbjct: 361 HTLFIKQGTTLDRSIGSHFKVPPASLYSFVTISMLLSILIYDRVFVKIMQRVTKNPRGIT 420

Query: 421 MVQRMGLGLICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQ--LPLTIFTLLPQFIL 480
           M+QRMG+G+ICHVL+MTVAS+VE+HRL +A  NGL    +  EQ  LPLTIF LLPQFIL
Sbjct: 421 MLQRMGIGMICHVLIMTVASQVEKHRLHIAEENGL---SAAQEQKVLPLTIFILLPQFIL 480

Query: 481 MGIADALLQIANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGNG 540
            GIADA LQIA+ EFFYDQAPE+MKSLG+SY +TS GIGNFLS+FILS VS ITK+ G G
Sbjct: 481 TGIADAFLQIASNEFFYDQAPENMKSLGNSYFMTSHGIGNFLSTFILSKVSKITKRQGKG 540

Query: 541 WILNNLNASHLDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEVLTDELKNKNFKG 600
           WILNNLNASHLDYFYALLAVMSAVNF LFL++S FY YKAEVSDSI+VLTDELK K  KG
Sbjct: 541 WILNNLNASHLDYFYALLAVMSAVNFGLFLLISKFYVYKAEVSDSIQVLTDELKKKKSKG 599

BLAST of Sed0000489 vs. NCBI nr
Match: XP_022143759.1 (protein NRT1/ PTR FAMILY 5.2-like [Momordica charantia])

HSP 1 Score: 929.5 bits (2401), Expect = 1.5e-266
Identity = 468/600 (78.00%), Postives = 539/600 (89.83%), Query Frame = 0

Query: 1   MAATATDRETG--HNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISV 60
           M   A + E G  ++DYT+DG+VDLKGNPVLR KTG WKACSFII YE+I+RMMFNGIS 
Sbjct: 1   MGGVAEEVEGGVVNDDYTQDGSVDLKGNPVLRRKTGRWKACSFIIVYEVIDRMMFNGISA 60

Query: 61  NLMIYLTTKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAM 120
           NL+IYLTTKLNQGTVTASNNVTNW+GT+W+ PVLGAYIADAHLGRYRT LIS+++C  +M
Sbjct: 61  NLIIYLTTKLNQGTVTASNNVTNWSGTLWITPVLGAYIADAHLGRYRTFLISTLLCFTSM 120

Query: 121 SVLTLAVSIPSLKPPPCQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGA 180
           +VLT+AVS+PSLKPPPC EANK+NCK QA+KLQL VFFG+LY LAVASGGTKPNIST+GA
Sbjct: 121 AVLTVAVSVPSLKPPPCLEANKENCK-QASKLQLGVFFGALYALAVASGGTKPNISTMGA 180

Query: 181 DQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAI 240
           DQFD+FDPKEKAQKLSFFNWWLF+VF GILFAST+LVYIQDNVGW LGYGIPT G+A AI
Sbjct: 181 DQFDEFDPKEKAQKLSFFNWWLFSVFFGILFASTVLVYIQDNVGWALGYGIPTSGLAVAI 240

Query: 241 LIFVCGTPFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTT 300
           LIF+ GTPFYRHKL S  G PF RMA VIVAAA+NW+LPLP DPK+L+EL+++HYSK  T
Sbjct: 241 LIFLAGTPFYRHKLPS--GSPFSRMAKVIVAAAFNWTLPLPKDPKQLHELDLEHYSKPGT 300

Query: 301 FKIDTTPSLRFLNKAAIRTNASNS----WRLCSVTQVEETKQMLRMIPILICTFMPSTMV 360
           F+ID+TPSLR LNKAAI+T +S++    W+LCSVTQVEETKQMLRMIPILICTF+PSTM+
Sbjct: 301 FRIDSTPSLRILNKAAIKTVSSSTTDHPWKLCSVTQVEETKQMLRMIPILICTFIPSTML 360

Query: 361 AQTHTLFIKQGTTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPR 420
           AQ HTLFIKQGTTLDRSIGSHF+VPPASLYAF+TISMLV+IV+YDR+FVKIMQ++TKNPR
Sbjct: 361 AQAHTLFIKQGTTLDRSIGSHFKVPPASLYAFVTISMLVTIVVYDRVFVKIMQRLTKNPR 420

Query: 421 GITMVQRMGLGLICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFI 480
           GIT++QRMG+G+I HVL+MTVASRVERHRL+VAR NGLV+NG GH  LPL+IFTLLPQF+
Sbjct: 421 GITLLQRMGIGMILHVLIMTVASRVERHRLDVARENGLVKNG-GHAVLPLSIFTLLPQFM 480

Query: 481 LMGIADALLQIANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGN 540
           LMGIADA +QIA+ EFFYDQAPE+MKSLGSSY +TSLGIGNFLSSF+LSTVS+IT K GN
Sbjct: 481 LMGIADAFVQIASVEFFYDQAPETMKSLGSSYMMTSLGIGNFLSSFLLSTVSDITNKRGN 540

Query: 541 GWILNNLNASHLDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEVLTDELKNKNFK 595
           GWI NNLNASHLDYFYALLAV++ VNF +FLVVS  Y YKAEVSDSI++LT+ LK K  K
Sbjct: 541 GWIQNNLNASHLDYFYALLAVLNVVNFCIFLVVSKMYVYKAEVSDSIKLLTEGLKKKKLK 596

BLAST of Sed0000489 vs. NCBI nr
Match: KAA0049221.1 (protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 922.5 bits (2383), Expect = 1.8e-264
Identity = 467/600 (77.83%), Postives = 532/600 (88.67%), Query Frame = 0

Query: 1   MAATATDRETG---HNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGIS 60
           MA  A ++E G   HN YTKDGTVD KGN VLRSKTG WKACSFI+GYELIERMMF+GIS
Sbjct: 1   MADAAANQEAGLDLHN-YTKDGTVDWKGNTVLRSKTGRWKACSFILGYELIERMMFHGIS 60

Query: 61  VNLMIYLTTKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVA 120
            NL+IYLTTKLNQGT+TASNNVTNW+GTVW MP++GAY+ADAHLGRYRT  ISS++C +A
Sbjct: 61  SNLIIYLTTKLNQGTLTASNNVTNWSGTVWTMPIIGAYVADAHLGRYRTFFISSLVCFMA 120

Query: 121 MSVLTLAVSIPSLKPPPCQEA-NKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTL 180
           M++LT AVSIPSLKPPPC  + +++NCK QA+KLQLAVFFGSLYLLA+ASGGTKPNIST+
Sbjct: 121 MTLLTAAVSIPSLKPPPCSASISRENCK-QASKLQLAVFFGSLYLLAIASGGTKPNISTM 180

Query: 181 GADQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAF 240
           GADQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGW LGYGIPTIG+  
Sbjct: 181 GADQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGV 240

Query: 241 AILIFVCGTPFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKS 300
           AILIFV GTPFYRHK     G PFI MANV VAA WNW LPLPNDP +L+EL++Q+YSK+
Sbjct: 241 AILIFVVGTPFYRHK--PPNGSPFITMANVFVAAIWNWRLPLPNDPNQLHELDLQNYSKN 300

Query: 301 TTFKIDTTPSLRFLNKAAIRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQ 360
            TFKID+TPSLRFLNKAAIR  +S+ WR+C+VT+VEETKQM+RMIPI++C+F+PS MVAQ
Sbjct: 301 GTFKIDSTPSLRFLNKAAIRRVSSDPWRICTVTEVEETKQMVRMIPIMVCSFIPSAMVAQ 360

Query: 361 THTLFIKQGTTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGI 420
           THTLFIKQGTTL+RSIGSHF+VPPASLYAF+TISML++I+IYDR+F+KIMQ+VTKNPRGI
Sbjct: 361 THTLFIKQGTTLNRSIGSHFKVPPASLYAFVTISMLLTILIYDRIFLKIMQRVTKNPRGI 420

Query: 421 TMVQRMGLGLICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQ--LPLTIFTLLPQFI 480
           TM+QRMG+G+ICHVLVMTVAS+VE+HRL +A   G     S HEQ  LPLTIF LLPQFI
Sbjct: 421 TMLQRMGIGMICHVLVMTVASQVEKHRLHIAAKYG----SSAHEQKELPLTIFILLPQFI 480

Query: 481 LMGIADALLQIANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGN 540
           L G+ADA L IAN EFFYDQAPE+MKSLGSSY  TSLGIGNFLS+FILS VS ITK+ GN
Sbjct: 481 LTGVADAFLLIANNEFFYDQAPENMKSLGSSYFTTSLGIGNFLSTFILSKVSEITKRQGN 540

Query: 541 GWILNNLNASHLDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEVLTDELKNKNFK 595
           GWILNNLN+SHL+YFYALLAVMS+VNFFLFL++S FY YKAEVSDSI+VLTDELK K  K
Sbjct: 541 GWILNNLNSSHLNYFYALLAVMSSVNFFLFLLISKFYVYKAEVSDSIQVLTDELKKKKSK 592

BLAST of Sed0000489 vs. NCBI nr
Match: XP_022924431.1 (protein NRT1/ PTR FAMILY 5.2-like [Cucurbita moschata])

HSP 1 Score: 907.5 bits (2344), Expect = 6.0e-260
Identity = 461/594 (77.61%), Postives = 520/594 (87.54%), Query Frame = 0

Query: 2   AATATDRETGHNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNLM 61
           AA A D+E+G +DYTKDGTVD KGNP LRS TG WKACSFI+ YELI+RMMFNGI+ NL+
Sbjct: 3   AAAAEDQESGLDDYTKDGTVDRKGNPTLRSNTGGWKACSFIVVYELIDRMMFNGIAANLI 62

Query: 62  IYLTTKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSVL 121
           IYLTTKLNQGTVTASNNVTNWTGTVW+ P+ GAY+ADAHLG YRT  ISS+   +AMS+L
Sbjct: 63  IYLTTKLNQGTVTASNNVTNWTGTVWITPIFGAYVADAHLGCYRTFFISSLASFMAMSLL 122

Query: 122 TLAVSIPSLKPPPCQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQF 181
           T+AVS+PSL+PPPC E +K+NCK QA+KLQLAVFFGSLY+LAVASGGTKPNIST+GADQF
Sbjct: 123 TVAVSVPSLQPPPCLEPSKENCK-QASKLQLAVFFGSLYMLAVASGGTKPNISTMGADQF 182

Query: 182 DDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILIF 241
           DDF PKEK+QKLSFFNWW+F+VFSGILFASTILVYIQDNVGW  GYGIPTIG+  AILIF
Sbjct: 183 DDFHPKEKSQKLSFFNWWMFSVFSGILFASTILVYIQDNVGWSFGYGIPTIGLGVAILIF 242

Query: 242 VCGTPFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFKI 301
           V GTPFYRH+L S  G PF+RMA VIVAAA NW +PLPNDP +LYELE+Q Y      KI
Sbjct: 243 VAGTPFYRHRLPS-GGSPFLRMARVIVAAARNWRVPLPNDPNQLYELEVQQYP-----KI 302

Query: 302 DTTPSLRFLNKAAIRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTLF 361
           D+TPS RFLNKAA+RT +S+ WR C+VTQVEETKQMLRMIPILICTF+PSTMVAQ+HTLF
Sbjct: 303 DSTPSFRFLNKAAVRTGSSHPWRSCTVTQVEETKQMLRMIPILICTFIPSTMVAQSHTLF 362

Query: 362 IKQGTTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQR 421
           IKQGTTLDR+IGSHF+VPPASLYAF+TISML+SIVIYDR+FVKIMQ+VT+NPRGITM+QR
Sbjct: 363 IKQGTTLDRTIGSHFKVPPASLYAFVTISMLLSIVIYDRIFVKIMQRVTRNPRGITMLQR 422

Query: 422 MGLGLICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIADA 481
           MG+G+I HVLVMTVASRVE+ RL VAR NGLV+NGSG + LPLTIFTLLPQF+L G+ADA
Sbjct: 423 MGIGMIFHVLVMTVASRVEKRRLHVARANGLVRNGSG-QVLPLTIFTLLPQFMLTGVADA 482

Query: 482 LLQIANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGNGWILNNL 541
           LLQIAN EFFYDQAP+SMKSLGSSY +TSLGIGNFLSSF+LS VS ITK+HG GWILNNL
Sbjct: 483 LLQIANVEFFYDQAPKSMKSLGSSYMMTSLGIGNFLSSFVLSKVSEITKRHGEGWILNNL 542

Query: 542 NASHLDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEVLTDELKNKNFKG 596
           NASHLDYFYALLA MS VNFF+FL +S  Y Y+AEVS       DEL  KN  G
Sbjct: 543 NASHLDYFYALLAAMSGVNFFVFLGISQLYVYRAEVS-------DELNKKNEVG 581

BLAST of Sed0000489 vs. ExPASy Swiss-Prot
Match: Q9FNL7 (Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 1.1e-208
Identity = 363/584 (62.16%), Postives = 456/584 (78.08%), Query Frame = 0

Query: 6   TDRETGHNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNLMIYLT 65
           T  E G +DYTKDGTVDL+GNPV RS  G WKACSF++ YE+ ERM + GIS NL IY+T
Sbjct: 2   TVEEVG-DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYMT 61

Query: 66  TKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSVLTLAV 125
           TKL+QGTV +SNNVTNW GT W+ P+LGAY+ DA LGRY T +IS  I    M VLTL+V
Sbjct: 62  TKLHQGTVKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLSV 121

Query: 126 SIPSLKPPPCQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQFDDFD 185
           +IP +KPP C   N +NC ++A+ LQLAVFFG+LY LA+ +GGTKPNIST+GADQFD FD
Sbjct: 122 TIPGIKPPECSTTNVENC-EKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFD 181

Query: 186 PKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILIFVCGT 245
           PKEK QKLSFFNWW+F++F G LFA+T+LVY+QDNVGW LGYG+PT+G+A +I IF+ GT
Sbjct: 182 PKEKTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGT 241

Query: 246 PFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFKIDTTP 305
           PFYRHKL +  G PF +MA VIVA+    + P+ +D    +EL    Y +   F I  TP
Sbjct: 242 PFYRHKLPT--GSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTP 301

Query: 306 SLRFLNKAAIRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTLFIKQG 365
           SLRFL++A+++T  ++ W LC+ T+VEETKQMLRM+P+L  TF+PS M+AQ +TLF+KQG
Sbjct: 302 SLRFLDRASLKTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQG 361

Query: 366 TTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQRMGLG 425
           TTLDR +   F +PPASL  F+T+SML+SIV+YDR+FVKI +K T NPRGIT++QRMG+G
Sbjct: 362 TTLDRKVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIG 421

Query: 426 LICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIADALLQI 485
           LI H+L+M VAS  ER+RL+VA  +GL+       +LPLTIF LLPQF+LMG+AD+ L++
Sbjct: 422 LIFHILIMIVASVTERYRLKVAADHGLIHQTG--VKLPLTIFALLPQFVLMGMADSFLEV 481

Query: 486 ANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGNGWILNNLNASH 545
           A  EFFYDQAPESMKSLG+SY+ TSL IGNF+SSF+LSTVS ITKK G GWILNNLN S 
Sbjct: 482 AKLEFFYDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNLNESR 541

Query: 546 LDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEVLTDELK 590
           LDY+Y   AV++ VNF LFLVV  FY Y+AEV+DS++V   E+K
Sbjct: 542 LDYYYLFFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMK 579

BLAST of Sed0000489 vs. ExPASy Swiss-Prot
Match: Q9FNL8 (Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 3.2e-203
Identity = 350/578 (60.55%), Postives = 452/578 (78.20%), Query Frame = 0

Query: 6   TDRETGHNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNLMIYLT 65
           T  E G +DYTKDGTVDL+GN V RS+TG WKACSF++ YE+ ERM + GIS NL+IY+T
Sbjct: 2   TVEEVG-DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMT 61

Query: 66  TKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSVLTLAV 125
           TKL+QGTV +SNNVTNW GT W+ P+LGAY+ADAH GRY T +ISS I L+ M++LTL+V
Sbjct: 62  TKLHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSV 121

Query: 126 SIPSLKPPPCQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQFDDFD 185
           S+P LKPP C  AN +NC ++A+ +QLAVFFG+LY LA+ +GGTKPNIST+GADQFD+FD
Sbjct: 122 SLPGLKPPKCSTANVENC-EKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFD 181

Query: 186 PKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILIFVCGT 245
           PK+K  K SFFNWW+F++F G  FA+T+LVY+QDNVGW +GYG+ T+G+AF+I IF+ GT
Sbjct: 182 PKDKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGT 241

Query: 246 PFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFKIDTTP 305
             YRHKL    G PF +MA VIVA+      P+ +D    YEL    Y+    F I +T 
Sbjct: 242 RLYRHKL--PMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTS 301

Query: 306 SLRFLNKAAIRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTLFIKQG 365
           SLRFLN+A+++T +++ WRLC++T+VEETKQML+M+P+L  TF+PS M+AQ  TLFIKQG
Sbjct: 302 SLRFLNRASLKTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQG 361

Query: 366 TTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQRMGLG 425
           TTLDR + ++F +PPASL  F T SMLVSIVIYDR+FVK M+K+T NPRGIT++QRMG+G
Sbjct: 362 TTLDRRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIG 421

Query: 426 LICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIADALLQI 485
           +I H+L+M +AS  ER+RL+VA  +GL    +    +PL+IFTLLPQ++LMG+ADA ++I
Sbjct: 422 MILHILIMIIASITERYRLKVAAEHGLTHQTA--VPIPLSIFTLLPQYVLMGLADAFIEI 481

Query: 486 ANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGNGWILNNLNASH 545
           A  EFFYDQAPESMKSLG+SYT TS+ +G F+SS +LS+VS ITKK G GWI NNLN S 
Sbjct: 482 AKLEFFYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNLNESR 541

Query: 546 LDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEV 584
           LD +Y   AV++ +NF LFLVV  FYEY+A+V+ S  V
Sbjct: 542 LDNYYMFFAVLNLLNFILFLVVIRFYEYRADVTQSANV 573

BLAST of Sed0000489 vs. ExPASy Swiss-Prot
Match: Q8VZR7 (Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2)

HSP 1 Score: 532.7 bits (1371), Expect = 5.3e-150
Identity = 275/563 (48.85%), Postives = 396/563 (70.34%), Query Frame = 0

Query: 15  YTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNLMIYLTTKLNQGTVT 74
           YT+DGTVDL+G PVL SKTG W+ACSF++GYE  ERM F GI+ NL+ YLT +L++ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 75  ASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSVLTLAVSIPSLKPPP 134
           +  NV NW+G VW+ P+ GAYIAD+++GR+ T   SS+I ++ M +LT+AV++ SL+ P 
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLR-PT 126

Query: 135 CQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQFDDFDPKEKAQKLS 194
           C+    +    +A+ LQ+  F+ SLY +A+ +GGTKPNIST GADQFD +  +EK QK+S
Sbjct: 127 CE----NGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVS 186

Query: 195 FFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILIFVCGTPFYRHKLIS 254
           FFNWW+F+ F G LFA+  LVYIQ+N+GWGLGYGIPT+G+  ++++F  GTPFYRHK+I 
Sbjct: 187 FFNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIK 246

Query: 255 YKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFKIDTTPSLRFLNKAA 314
                   +  V +AA  N  L  P+D  ELYEL+  +Y  +   ++  TP  RFL+KAA
Sbjct: 247 -TDNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAA 306

Query: 315 IRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTLFIKQGTTLDRSIGS 374
           I+T++      C+VT+VE  K++L +I I + T +PST+ AQ +TLF+KQGTTLDR IGS
Sbjct: 307 IKTSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRKIGS 366

Query: 375 HFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQRMGLGLICHVLVMT 434
           +F++P ASL +F+T+SML+S+ +YD+ FV  M+K T NPRGIT++QR+G+G    ++ + 
Sbjct: 367 NFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIVAIA 426

Query: 435 VASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIADALLQIANTEFFYDQ 494
           +AS VE  R+ V +   +    S  + +P++IF LLPQ+ L+GI D    I   EFFYDQ
Sbjct: 427 IASAVEVKRMRVIKEFHIT---SPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQ 486

Query: 495 APESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKK-HGNGWILNNLNASHLDYFYALL 554
           +PE M+SLG+++  + +G+GNFL+SF+++ +  IT K  G  WI NNLN S LDY+Y  L
Sbjct: 487 SPEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGFL 546

Query: 555 AVMSAVNFFLFLVVSHFYEYKAE 577
            V+S VN  LF+  +  Y YK++
Sbjct: 547 VVISIVNMGLFVWAASKYVYKSD 557

BLAST of Sed0000489 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 1.0e-132
Identity = 250/571 (43.78%), Postives = 374/571 (65.50%), Query Frame = 0

Query: 15  YTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNLMIYLTTKLNQGTVT 74
           YT+DGTVD+  NP  + KTG+WKAC FI+G E  ER+ + G+  NL+ YL ++LNQG  T
Sbjct: 7   YTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNAT 66

Query: 75  ASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSVLTLAVSIPSLKPPP 134
           A+NNVTNW+GT ++ P++GA+IADA+LGRY T+     I +  M++LTL+ S+P LKP  
Sbjct: 67  AANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGN 126

Query: 135 CQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQFDDFDPKEKAQKLS 194
           C   N D C   ++  Q AVFF +LY++A+ +GG KP +S+ GADQFD+ D  EK +K S
Sbjct: 127 C---NADTCHPNSS--QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSS 186

Query: 195 FFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILIFVCGTPFYRHKLIS 254
           FFNW+ F++  G L A+T+LV+IQ NVGWG G+G+PT+ +  A+  F  G+ FYR  L  
Sbjct: 187 FFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYR--LQR 246

Query: 255 YKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFKIDTTPSLRFLNKAA 314
             G P  R+  VIVAA    S+ +P D   L+E      +   + K+  T +L+F +KAA
Sbjct: 247 PGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAA 306

Query: 315 IRTNAS-------NSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTLFIKQGTT 374
           + + +        N WRLCSVTQVEE K ++ ++P+     + +T+ +Q  T+F+ QG T
Sbjct: 307 VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 366

Query: 375 LDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQRMGLGLI 434
           +D+ +G +FE+P ASL  F T+S+L    +YD+  + + +K T+N RG T +QRMG+GL+
Sbjct: 367 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 426

Query: 435 CHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIADALLQIAN 494
             +  M  A  +E  RL+  +T+    N    +Q+ ++IF  +PQ++L+G A+    I  
Sbjct: 427 VSIFAMITAGVLEVVRLDYVKTH----NAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQ 486

Query: 495 TEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGN-GWILNNLNASHL 554
            EFFYDQAP++M+SL S+ +LT++ +GN+LS+ +++ V  ITKK+G  GWI +NLN  HL
Sbjct: 487 LEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHL 546

Query: 555 DYFYALLAVMSAVNFFLFLVVSHFYEYKAEV 578
           DYF+ LLA +S +NF ++L +S  Y+YK  V
Sbjct: 547 DYFFYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Sed0000489 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 8.8e-129
Identity = 253/568 (44.54%), Postives = 373/568 (65.67%), Query Frame = 0

Query: 15  YTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNLMIYLTTKLNQGTVT 74
           Y +DG+VD  GNP L+ KTG+WKAC FI+G E  ER+ + GI+ NL+ YLTTKL+QG V+
Sbjct: 24  YAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVS 83

Query: 75  ASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSVLTLAVSIPSLKPPP 134
           A+ NVT W GT ++ P++GA +ADA+ GRY T+   S I  + MS LTL+ S+P+LKP  
Sbjct: 84  AATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE 143

Query: 135 CQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQFDDFDPKEKAQKLS 194
           C     D C   AT  Q A+FFG LYL+A+ +GG KP +S+ GADQFDD D +E+ +K S
Sbjct: 144 C---IGDFC-PSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKAS 203

Query: 195 FFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILIFVCGTPFYRHKLIS 254
           FFNW+ F++  G L +S++LV+IQ+N GWGLG+GIPT+ +  AI  F  GTP YR +   
Sbjct: 204 FFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQ--K 263

Query: 255 YKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFKIDTTPSLRFLNKAA 314
             G P  R++ V+VA+    S+ +P D   LYE + ++ + + + KI+ T   ++L+KAA
Sbjct: 264 PGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAA 323

Query: 315 IRT-------NASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTLFIKQGTT 374
           + +       + SNSWRLC+VTQVEE K ++RM PI     + S + AQ  T+F++QG  
Sbjct: 324 VISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRA 383

Query: 375 LDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQRMGLGLI 434
           ++  IGS F++PPA+L  F T S+++ + +YDR  V + +K T   +G T +QRMG+GL 
Sbjct: 384 MNCKIGS-FQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLF 443

Query: 435 CHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIADALLQIAN 494
             VL M  A+ VE  RL +A   GLV++G+    +P+++   +PQ+ ++G A+    I  
Sbjct: 444 VSVLCMAAAAIVEIIRLHMANDLGLVESGA---PVPISVLWQIPQYFILGAAEVFYFIGQ 503

Query: 495 TEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHG-NGWILNNLNASHL 554
            EFFYDQ+P++M+SL S+  L +  +GN+LSS IL+ V+  T ++G  GWI +NLN+ HL
Sbjct: 504 LEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHL 563

Query: 555 DYFYALLAVMSAVNFFLFLVVSHFYEYK 575
           DYF+ LLA +S VN  ++   +  Y+ K
Sbjct: 564 DYFFWLLAGLSLVNMAVYFFSAARYKQK 581

BLAST of Sed0000489 vs. ExPASy TrEMBL
Match: A0A6J1CQA1 (protein NRT1/ PTR FAMILY 5.2-like OS=Momordica charantia OX=3673 GN=LOC111013596 PE=3 SV=1)

HSP 1 Score: 929.5 bits (2401), Expect = 7.2e-267
Identity = 468/600 (78.00%), Postives = 539/600 (89.83%), Query Frame = 0

Query: 1   MAATATDRETG--HNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISV 60
           M   A + E G  ++DYT+DG+VDLKGNPVLR KTG WKACSFII YE+I+RMMFNGIS 
Sbjct: 1   MGGVAEEVEGGVVNDDYTQDGSVDLKGNPVLRRKTGRWKACSFIIVYEVIDRMMFNGISA 60

Query: 61  NLMIYLTTKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAM 120
           NL+IYLTTKLNQGTVTASNNVTNW+GT+W+ PVLGAYIADAHLGRYRT LIS+++C  +M
Sbjct: 61  NLIIYLTTKLNQGTVTASNNVTNWSGTLWITPVLGAYIADAHLGRYRTFLISTLLCFTSM 120

Query: 121 SVLTLAVSIPSLKPPPCQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGA 180
           +VLT+AVS+PSLKPPPC EANK+NCK QA+KLQL VFFG+LY LAVASGGTKPNIST+GA
Sbjct: 121 AVLTVAVSVPSLKPPPCLEANKENCK-QASKLQLGVFFGALYALAVASGGTKPNISTMGA 180

Query: 181 DQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAI 240
           DQFD+FDPKEKAQKLSFFNWWLF+VF GILFAST+LVYIQDNVGW LGYGIPT G+A AI
Sbjct: 181 DQFDEFDPKEKAQKLSFFNWWLFSVFFGILFASTVLVYIQDNVGWALGYGIPTSGLAVAI 240

Query: 241 LIFVCGTPFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTT 300
           LIF+ GTPFYRHKL S  G PF RMA VIVAAA+NW+LPLP DPK+L+EL+++HYSK  T
Sbjct: 241 LIFLAGTPFYRHKLPS--GSPFSRMAKVIVAAAFNWTLPLPKDPKQLHELDLEHYSKPGT 300

Query: 301 FKIDTTPSLRFLNKAAIRTNASNS----WRLCSVTQVEETKQMLRMIPILICTFMPSTMV 360
           F+ID+TPSLR LNKAAI+T +S++    W+LCSVTQVEETKQMLRMIPILICTF+PSTM+
Sbjct: 301 FRIDSTPSLRILNKAAIKTVSSSTTDHPWKLCSVTQVEETKQMLRMIPILICTFIPSTML 360

Query: 361 AQTHTLFIKQGTTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPR 420
           AQ HTLFIKQGTTLDRSIGSHF+VPPASLYAF+TISMLV+IV+YDR+FVKIMQ++TKNPR
Sbjct: 361 AQAHTLFIKQGTTLDRSIGSHFKVPPASLYAFVTISMLVTIVVYDRVFVKIMQRLTKNPR 420

Query: 421 GITMVQRMGLGLICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFI 480
           GIT++QRMG+G+I HVL+MTVASRVERHRL+VAR NGLV+NG GH  LPL+IFTLLPQF+
Sbjct: 421 GITLLQRMGIGMILHVLIMTVASRVERHRLDVARENGLVKNG-GHAVLPLSIFTLLPQFM 480

Query: 481 LMGIADALLQIANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGN 540
           LMGIADA +QIA+ EFFYDQAPE+MKSLGSSY +TSLGIGNFLSSF+LSTVS+IT K GN
Sbjct: 481 LMGIADAFVQIASVEFFYDQAPETMKSLGSSYMMTSLGIGNFLSSFLLSTVSDITNKRGN 540

Query: 541 GWILNNLNASHLDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEVLTDELKNKNFK 595
           GWI NNLNASHLDYFYALLAV++ VNF +FLVVS  Y YKAEVSDSI++LT+ LK K  K
Sbjct: 541 GWIQNNLNASHLDYFYALLAVLNVVNFCIFLVVSKMYVYKAEVSDSIKLLTEGLKKKKLK 596

BLAST of Sed0000489 vs. ExPASy TrEMBL
Match: A0A5A7U6G8 (Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G004610 PE=3 SV=1)

HSP 1 Score: 922.5 bits (2383), Expect = 8.8e-265
Identity = 467/600 (77.83%), Postives = 532/600 (88.67%), Query Frame = 0

Query: 1   MAATATDRETG---HNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGIS 60
           MA  A ++E G   HN YTKDGTVD KGN VLRSKTG WKACSFI+GYELIERMMF+GIS
Sbjct: 1   MADAAANQEAGLDLHN-YTKDGTVDWKGNTVLRSKTGRWKACSFILGYELIERMMFHGIS 60

Query: 61  VNLMIYLTTKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVA 120
            NL+IYLTTKLNQGT+TASNNVTNW+GTVW MP++GAY+ADAHLGRYRT  ISS++C +A
Sbjct: 61  SNLIIYLTTKLNQGTLTASNNVTNWSGTVWTMPIIGAYVADAHLGRYRTFFISSLVCFMA 120

Query: 121 MSVLTLAVSIPSLKPPPCQEA-NKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTL 180
           M++LT AVSIPSLKPPPC  + +++NCK QA+KLQLAVFFGSLYLLA+ASGGTKPNIST+
Sbjct: 121 MTLLTAAVSIPSLKPPPCSASISRENCK-QASKLQLAVFFGSLYLLAIASGGTKPNISTM 180

Query: 181 GADQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAF 240
           GADQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGW LGYGIPTIG+  
Sbjct: 181 GADQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGV 240

Query: 241 AILIFVCGTPFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKS 300
           AILIFV GTPFYRHK     G PFI MANV VAA WNW LPLPNDP +L+EL++Q+YSK+
Sbjct: 241 AILIFVVGTPFYRHK--PPNGSPFITMANVFVAAIWNWRLPLPNDPNQLHELDLQNYSKN 300

Query: 301 TTFKIDTTPSLRFLNKAAIRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQ 360
            TFKID+TPSLRFLNKAAIR  +S+ WR+C+VT+VEETKQM+RMIPI++C+F+PS MVAQ
Sbjct: 301 GTFKIDSTPSLRFLNKAAIRRVSSDPWRICTVTEVEETKQMVRMIPIMVCSFIPSAMVAQ 360

Query: 361 THTLFIKQGTTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGI 420
           THTLFIKQGTTL+RSIGSHF+VPPASLYAF+TISML++I+IYDR+F+KIMQ+VTKNPRGI
Sbjct: 361 THTLFIKQGTTLNRSIGSHFKVPPASLYAFVTISMLLTILIYDRIFLKIMQRVTKNPRGI 420

Query: 421 TMVQRMGLGLICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQ--LPLTIFTLLPQFI 480
           TM+QRMG+G+ICHVLVMTVAS+VE+HRL +A   G     S HEQ  LPLTIF LLPQFI
Sbjct: 421 TMLQRMGIGMICHVLVMTVASQVEKHRLHIAAKYG----SSAHEQKELPLTIFILLPQFI 480

Query: 481 LMGIADALLQIANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGN 540
           L G+ADA L IAN EFFYDQAPE+MKSLGSSY  TSLGIGNFLS+FILS VS ITK+ GN
Sbjct: 481 LTGVADAFLLIANNEFFYDQAPENMKSLGSSYFTTSLGIGNFLSTFILSKVSEITKRQGN 540

Query: 541 GWILNNLNASHLDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEVLTDELKNKNFK 595
           GWILNNLN+SHL+YFYALLAVMS+VNFFLFL++S FY YKAEVSDSI+VLTDELK K  K
Sbjct: 541 GWILNNLNSSHLNYFYALLAVMSSVNFFLFLLISKFYVYKAEVSDSIQVLTDELKKKKSK 592

BLAST of Sed0000489 vs. ExPASy TrEMBL
Match: A0A6J1E9F4 (protein NRT1/ PTR FAMILY 5.2-like OS=Cucurbita moschata OX=3662 GN=LOC111431936 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.9e-260
Identity = 461/594 (77.61%), Postives = 520/594 (87.54%), Query Frame = 0

Query: 2   AATATDRETGHNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNLM 61
           AA A D+E+G +DYTKDGTVD KGNP LRS TG WKACSFI+ YELI+RMMFNGI+ NL+
Sbjct: 3   AAAAEDQESGLDDYTKDGTVDRKGNPTLRSNTGGWKACSFIVVYELIDRMMFNGIAANLI 62

Query: 62  IYLTTKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSVL 121
           IYLTTKLNQGTVTASNNVTNWTGTVW+ P+ GAY+ADAHLG YRT  ISS+   +AMS+L
Sbjct: 63  IYLTTKLNQGTVTASNNVTNWTGTVWITPIFGAYVADAHLGCYRTFFISSLASFMAMSLL 122

Query: 122 TLAVSIPSLKPPPCQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQF 181
           T+AVS+PSL+PPPC E +K+NCK QA+KLQLAVFFGSLY+LAVASGGTKPNIST+GADQF
Sbjct: 123 TVAVSVPSLQPPPCLEPSKENCK-QASKLQLAVFFGSLYMLAVASGGTKPNISTMGADQF 182

Query: 182 DDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILIF 241
           DDF PKEK+QKLSFFNWW+F+VFSGILFASTILVYIQDNVGW  GYGIPTIG+  AILIF
Sbjct: 183 DDFHPKEKSQKLSFFNWWMFSVFSGILFASTILVYIQDNVGWSFGYGIPTIGLGVAILIF 242

Query: 242 VCGTPFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFKI 301
           V GTPFYRH+L S  G PF+RMA VIVAAA NW +PLPNDP +LYELE+Q Y      KI
Sbjct: 243 VAGTPFYRHRLPS-GGSPFLRMARVIVAAARNWRVPLPNDPNQLYELEVQQYP-----KI 302

Query: 302 DTTPSLRFLNKAAIRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTLF 361
           D+TPS RFLNKAA+RT +S+ WR C+VTQVEETKQMLRMIPILICTF+PSTMVAQ+HTLF
Sbjct: 303 DSTPSFRFLNKAAVRTGSSHPWRSCTVTQVEETKQMLRMIPILICTFIPSTMVAQSHTLF 362

Query: 362 IKQGTTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQR 421
           IKQGTTLDR+IGSHF+VPPASLYAF+TISML+SIVIYDR+FVKIMQ+VT+NPRGITM+QR
Sbjct: 363 IKQGTTLDRTIGSHFKVPPASLYAFVTISMLLSIVIYDRIFVKIMQRVTRNPRGITMLQR 422

Query: 422 MGLGLICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIADA 481
           MG+G+I HVLVMTVASRVE+ RL VAR NGLV+NGSG + LPLTIFTLLPQF+L G+ADA
Sbjct: 423 MGIGMIFHVLVMTVASRVEKRRLHVARANGLVRNGSG-QVLPLTIFTLLPQFMLTGVADA 482

Query: 482 LLQIANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGNGWILNNL 541
           LLQIAN EFFYDQAP+SMKSLGSSY +TSLGIGNFLSSF+LS VS ITK+HG GWILNNL
Sbjct: 483 LLQIANVEFFYDQAPKSMKSLGSSYMMTSLGIGNFLSSFVLSKVSEITKRHGEGWILNNL 542

Query: 542 NASHLDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEVLTDELKNKNFKG 596
           NASHLDYFYALLA MS VNFF+FL +S  Y Y+AEVS       DEL  KN  G
Sbjct: 543 NASHLDYFYALLAAMSGVNFFVFLGISQLYVYRAEVS-------DELNKKNEVG 581

BLAST of Sed0000489 vs. ExPASy TrEMBL
Match: A0A6J1IQK5 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479112 PE=3 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 6.5e-260
Identity = 445/594 (74.92%), Postives = 527/594 (88.72%), Query Frame = 0

Query: 1   MAATATDRETGHNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNL 60
           MAA+A + E+G +DYTKDGTVDLKGNPVLRSK G WKACSFI+ YE+ ERM + GIS NL
Sbjct: 1   MAASAAE-ESGVDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNL 60

Query: 61  MIYLTTKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSV 120
           +I+LT KL+QGTV ++NNVTNW+GTVW+MP+LGAYIADAHLGRYRT LI+S ICL  M +
Sbjct: 61  IIFLTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGL 120

Query: 121 LTLAVSIPSLKPPPCQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQ 180
           LTLAVS+PSLKPPPC + NK NCK  +T LQLAVFFG+LY+LA+ +GGTKPNIST+GADQ
Sbjct: 121 LTLAVSLPSLKPPPCLDVNKGNCKPAST-LQLAVFFGALYMLALGTGGTKPNISTIGADQ 180

Query: 181 FDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILI 240
           FD+F PKEKAQKLSFFNWW+F++F G LFA+TILVYIQDNVGW LGYG+PTIG+A +ILI
Sbjct: 181 FDEFHPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILI 240

Query: 241 FVCGTPFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFK 300
           FV GTPFYRHKL +  G PF +MA+VIVAA  NW LPLPNDPKEL+EL  + Y+K   F+
Sbjct: 241 FVAGTPFYRHKLPT--GSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFR 300

Query: 301 IDTTPSLRFLNKAAIRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTL 360
           ID+TPSLRFLNKAAIR  +S+SW+LC+VTQVEETKQMLRMIP+LICTFMPSTM+AQTHTL
Sbjct: 301 IDSTPSLRFLNKAAIRRGSSDSWKLCTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTL 360

Query: 361 FIKQGTTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQ 420
           FIKQGTTLDRSIGSHF++PPASL AF+TISML+S+VIYDR+FVKIMQ++TKNPRGIT++Q
Sbjct: 361 FIKQGTTLDRSIGSHFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQ 420

Query: 421 RMGLGLICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIAD 480
           RMG+G+I HVL+M +ASRVERHRL+VAR NG V NGSG + LPLTIFTLLPQF+L+G+AD
Sbjct: 421 RMGIGMILHVLIMIIASRVERHRLDVARQNGQVGNGSG-QALPLTIFTLLPQFMLVGVAD 480

Query: 481 ALLQIANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGNGWILNN 540
           A  ++A  EFFYDQAPESMKSLG+SY++TS+GIGNFLSSF+LSTVS+IT KHGNGWI+NN
Sbjct: 481 AFTEVAKIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKHGNGWIMNN 540

Query: 541 LNASHLDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEVLTDELKNKNFK 595
           LNASHLDY+YA LAV+SA+NFFLFL++S FY YKAEVS SI+ L DELK+K  K
Sbjct: 541 LNASHLDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKALADELKDKKLK 589

BLAST of Sed0000489 vs. ExPASy TrEMBL
Match: A0A6J1E956 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431935 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 9.4e-259
Identity = 444/594 (74.75%), Postives = 526/594 (88.55%), Query Frame = 0

Query: 1   MAATATDRETGHNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNL 60
           MAA+A + E+G +DYTKDGTVDLKGNPVLRSK G WKACSFI+ YE+ ERM + GIS NL
Sbjct: 1   MAASAAE-ESGVDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNL 60

Query: 61  MIYLTTKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSV 120
           +I+LT KL+QGTV ++NNVTNW+GTVW+MP+LGAYIADAHLGRYRT LI+S ICL  M +
Sbjct: 61  IIFLTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGL 120

Query: 121 LTLAVSIPSLKPPPCQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQ 180
           LTLAVS+PSLKPPPC + NK NCK  +T LQLAVFFG+LY+LA+ +GGTKPNIST+GADQ
Sbjct: 121 LTLAVSLPSLKPPPCLDVNKGNCKPAST-LQLAVFFGALYMLALGTGGTKPNISTIGADQ 180

Query: 181 FDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILI 240
           FD+F PKEKAQKLSFFNWW+F++F G LFA+TILVYIQDNVGW LGYG+PTIG+A +ILI
Sbjct: 181 FDEFHPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILI 240

Query: 241 FVCGTPFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFK 300
           FV GTPFYRHKL +  G PF +MA+VIVAA  NW LPLPNDPKEL+EL  + Y+K   F+
Sbjct: 241 FVAGTPFYRHKLPT--GSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFR 300

Query: 301 IDTTPSLRFLNKAAIRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTL 360
           ID+TPSLRFLNKAAIR  +S+SW+LC+VTQVEETKQMLRMIP+LICTFMPSTM+AQTHTL
Sbjct: 301 IDSTPSLRFLNKAAIRRGSSDSWKLCTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTL 360

Query: 361 FIKQGTTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQ 420
           FIKQGTTLDRSIGSHF++PPASL AF+TISML+S+VIYDR+FVKIMQ++TKNPRGIT++Q
Sbjct: 361 FIKQGTTLDRSIGSHFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQ 420

Query: 421 RMGLGLICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIAD 480
           RMG+G+I HVL+M +ASRVERHRL+VAR NG V N SG + LPLTIFTLLPQF+L+G+AD
Sbjct: 421 RMGIGMILHVLIMIIASRVERHRLDVARQNGQVGNASG-QALPLTIFTLLPQFMLVGVAD 480

Query: 481 ALLQIANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGNGWILNN 540
           A  ++A  EFFYDQAPESMKSLG+SY++TS+GIGNFLSSF+LSTVS+IT K GNGWI+NN
Sbjct: 481 AFTEVAKIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKRGNGWIMNN 540

Query: 541 LNASHLDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEVLTDELKNKNFK 595
           LNASHLDY+YA LAV+SA+NFFLFL++S FY YKAEVS SI+VL DELK+K  K
Sbjct: 541 LNASHLDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKVLADELKDKKLK 589

BLAST of Sed0000489 vs. TAIR 10
Match: AT5G46050.1 (peptide transporter 3 )

HSP 1 Score: 727.6 bits (1877), Expect = 8.0e-210
Identity = 363/584 (62.16%), Postives = 456/584 (78.08%), Query Frame = 0

Query: 6   TDRETGHNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNLMIYLT 65
           T  E G +DYTKDGTVDL+GNPV RS  G WKACSF++ YE+ ERM + GIS NL IY+T
Sbjct: 2   TVEEVG-DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYMT 61

Query: 66  TKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSVLTLAV 125
           TKL+QGTV +SNNVTNW GT W+ P+LGAY+ DA LGRY T +IS  I    M VLTL+V
Sbjct: 62  TKLHQGTVKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLSV 121

Query: 126 SIPSLKPPPCQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQFDDFD 185
           +IP +KPP C   N +NC ++A+ LQLAVFFG+LY LA+ +GGTKPNIST+GADQFD FD
Sbjct: 122 TIPGIKPPECSTTNVENC-EKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFD 181

Query: 186 PKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILIFVCGT 245
           PKEK QKLSFFNWW+F++F G LFA+T+LVY+QDNVGW LGYG+PT+G+A +I IF+ GT
Sbjct: 182 PKEKTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGT 241

Query: 246 PFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFKIDTTP 305
           PFYRHKL +  G PF +MA VIVA+    + P+ +D    +EL    Y +   F I  TP
Sbjct: 242 PFYRHKLPT--GSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTP 301

Query: 306 SLRFLNKAAIRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTLFIKQG 365
           SLRFL++A+++T  ++ W LC+ T+VEETKQMLRM+P+L  TF+PS M+AQ +TLF+KQG
Sbjct: 302 SLRFLDRASLKTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQG 361

Query: 366 TTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQRMGLG 425
           TTLDR +   F +PPASL  F+T+SML+SIV+YDR+FVKI +K T NPRGIT++QRMG+G
Sbjct: 362 TTLDRKVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIG 421

Query: 426 LICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIADALLQI 485
           LI H+L+M VAS  ER+RL+VA  +GL+       +LPLTIF LLPQF+LMG+AD+ L++
Sbjct: 422 LIFHILIMIVASVTERYRLKVAADHGLIHQTG--VKLPLTIFALLPQFVLMGMADSFLEV 481

Query: 486 ANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGNGWILNNLNASH 545
           A  EFFYDQAPESMKSLG+SY+ TSL IGNF+SSF+LSTVS ITKK G GWILNNLN S 
Sbjct: 482 AKLEFFYDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNLNESR 541

Query: 546 LDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEVLTDELK 590
           LDY+Y   AV++ VNF LFLVV  FY Y+AEV+DS++V   E+K
Sbjct: 542 LDYYYLFFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMK 579

BLAST of Sed0000489 vs. TAIR 10
Match: AT5G46040.1 (Major facilitator superfamily protein )

HSP 1 Score: 709.5 bits (1830), Expect = 2.2e-204
Identity = 350/578 (60.55%), Postives = 452/578 (78.20%), Query Frame = 0

Query: 6   TDRETGHNDYTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNLMIYLT 65
           T  E G +DYTKDGTVDL+GN V RS+TG WKACSF++ YE+ ERM + GIS NL+IY+T
Sbjct: 2   TVEEVG-DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMT 61

Query: 66  TKLNQGTVTASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSVLTLAV 125
           TKL+QGTV +SNNVTNW GT W+ P+LGAY+ADAH GRY T +ISS I L+ M++LTL+V
Sbjct: 62  TKLHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSV 121

Query: 126 SIPSLKPPPCQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQFDDFD 185
           S+P LKPP C  AN +NC ++A+ +QLAVFFG+LY LA+ +GGTKPNIST+GADQFD+FD
Sbjct: 122 SLPGLKPPKCSTANVENC-EKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFD 181

Query: 186 PKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILIFVCGT 245
           PK+K  K SFFNWW+F++F G  FA+T+LVY+QDNVGW +GYG+ T+G+AF+I IF+ GT
Sbjct: 182 PKDKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGT 241

Query: 246 PFYRHKLISYKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFKIDTTP 305
             YRHKL    G PF +MA VIVA+      P+ +D    YEL    Y+    F I +T 
Sbjct: 242 RLYRHKL--PMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTS 301

Query: 306 SLRFLNKAAIRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTLFIKQG 365
           SLRFLN+A+++T +++ WRLC++T+VEETKQML+M+P+L  TF+PS M+AQ  TLFIKQG
Sbjct: 302 SLRFLNRASLKTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQG 361

Query: 366 TTLDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQRMGLG 425
           TTLDR + ++F +PPASL  F T SMLVSIVIYDR+FVK M+K+T NPRGIT++QRMG+G
Sbjct: 362 TTLDRRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIG 421

Query: 426 LICHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIADALLQI 485
           +I H+L+M +AS  ER+RL+VA  +GL    +    +PL+IFTLLPQ++LMG+ADA ++I
Sbjct: 422 MILHILIMIIASITERYRLKVAAEHGLTHQTA--VPIPLSIFTLLPQYVLMGLADAFIEI 481

Query: 486 ANTEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGNGWILNNLNASH 545
           A  EFFYDQAPESMKSLG+SYT TS+ +G F+SS +LS+VS ITKK G GWI NNLN S 
Sbjct: 482 AKLEFFYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNLNESR 541

Query: 546 LDYFYALLAVMSAVNFFLFLVVSHFYEYKAEVSDSIEV 584
           LD +Y   AV++ +NF LFLVV  FYEY+A+V+ S  V
Sbjct: 542 LDNYYMFFAVLNLLNFILFLVVIRFYEYRADVTQSANV 573

BLAST of Sed0000489 vs. TAIR 10
Match: AT2G40460.1 (Major facilitator superfamily protein )

HSP 1 Score: 532.7 bits (1371), Expect = 3.8e-151
Identity = 275/563 (48.85%), Postives = 396/563 (70.34%), Query Frame = 0

Query: 15  YTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNLMIYLTTKLNQGTVT 74
           YT+DGTVDL+G PVL SKTG W+ACSF++GYE  ERM F GI+ NL+ YLT +L++ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 75  ASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSVLTLAVSIPSLKPPP 134
           +  NV NW+G VW+ P+ GAYIAD+++GR+ T   SS+I ++ M +LT+AV++ SL+ P 
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLR-PT 126

Query: 135 CQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQFDDFDPKEKAQKLS 194
           C+    +    +A+ LQ+  F+ SLY +A+ +GGTKPNIST GADQFD +  +EK QK+S
Sbjct: 127 CE----NGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVS 186

Query: 195 FFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILIFVCGTPFYRHKLIS 254
           FFNWW+F+ F G LFA+  LVYIQ+N+GWGLGYGIPT+G+  ++++F  GTPFYRHK+I 
Sbjct: 187 FFNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIK 246

Query: 255 YKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFKIDTTPSLRFLNKAA 314
                   +  V +AA  N  L  P+D  ELYEL+  +Y  +   ++  TP  RFL+KAA
Sbjct: 247 -TDNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAA 306

Query: 315 IRTNASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTLFIKQGTTLDRSIGS 374
           I+T++      C+VT+VE  K++L +I I + T +PST+ AQ +TLF+KQGTTLDR IGS
Sbjct: 307 IKTSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRKIGS 366

Query: 375 HFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQRMGLGLICHVLVMT 434
           +F++P ASL +F+T+SML+S+ +YD+ FV  M+K T NPRGIT++QR+G+G    ++ + 
Sbjct: 367 NFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIVAIA 426

Query: 435 VASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIADALLQIANTEFFYDQ 494
           +AS VE  R+ V +   +    S  + +P++IF LLPQ+ L+GI D    I   EFFYDQ
Sbjct: 427 IASAVEVKRMRVIKEFHIT---SPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQ 486

Query: 495 APESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKK-HGNGWILNNLNASHLDYFYALL 554
           +PE M+SLG+++  + +G+GNFL+SF+++ +  IT K  G  WI NNLN S LDY+Y  L
Sbjct: 487 SPEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGFL 546

Query: 555 AVMSAVNFFLFLVVSHFYEYKAE 577
            V+S VN  LF+  +  Y YK++
Sbjct: 547 VVISIVNMGLFVWAASKYVYKSD 557

BLAST of Sed0000489 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 475.3 bits (1222), Expect = 7.1e-134
Identity = 250/571 (43.78%), Postives = 374/571 (65.50%), Query Frame = 0

Query: 15  YTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNLMIYLTTKLNQGTVT 74
           YT+DGTVD+  NP  + KTG+WKAC FI+G E  ER+ + G+  NL+ YL ++LNQG  T
Sbjct: 7   YTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNAT 66

Query: 75  ASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSVLTLAVSIPSLKPPP 134
           A+NNVTNW+GT ++ P++GA+IADA+LGRY T+     I +  M++LTL+ S+P LKP  
Sbjct: 67  AANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGN 126

Query: 135 CQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQFDDFDPKEKAQKLS 194
           C   N D C   ++  Q AVFF +LY++A+ +GG KP +S+ GADQFD+ D  EK +K S
Sbjct: 127 C---NADTCHPNSS--QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSS 186

Query: 195 FFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILIFVCGTPFYRHKLIS 254
           FFNW+ F++  G L A+T+LV+IQ NVGWG G+G+PT+ +  A+  F  G+ FYR  L  
Sbjct: 187 FFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYR--LQR 246

Query: 255 YKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFKIDTTPSLRFLNKAA 314
             G P  R+  VIVAA    S+ +P D   L+E      +   + K+  T +L+F +KAA
Sbjct: 247 PGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAA 306

Query: 315 IRTNAS-------NSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTLFIKQGTT 374
           + + +        N WRLCSVTQVEE K ++ ++P+     + +T+ +Q  T+F+ QG T
Sbjct: 307 VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 366

Query: 375 LDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQRMGLGLI 434
           +D+ +G +FE+P ASL  F T+S+L    +YD+  + + +K T+N RG T +QRMG+GL+
Sbjct: 367 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 426

Query: 435 CHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIADALLQIAN 494
             +  M  A  +E  RL+  +T+    N    +Q+ ++IF  +PQ++L+G A+    I  
Sbjct: 427 VSIFAMITAGVLEVVRLDYVKTH----NAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQ 486

Query: 495 TEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHGN-GWILNNLNASHL 554
            EFFYDQAP++M+SL S+ +LT++ +GN+LS+ +++ V  ITKK+G  GWI +NLN  HL
Sbjct: 487 LEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHL 546

Query: 555 DYFYALLAVMSAVNFFLFLVVSHFYEYKAEV 578
           DYF+ LLA +S +NF ++L +S  Y+YK  V
Sbjct: 547 DYFFYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Sed0000489 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 462.2 bits (1188), Expect = 6.2e-130
Identity = 253/568 (44.54%), Postives = 373/568 (65.67%), Query Frame = 0

Query: 15  YTKDGTVDLKGNPVLRSKTGHWKACSFIIGYELIERMMFNGISVNLMIYLTTKLNQGTVT 74
           Y +DG+VD  GNP L+ KTG+WKAC FI+G E  ER+ + GI+ NL+ YLTTKL+QG V+
Sbjct: 24  YAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVS 83

Query: 75  ASNNVTNWTGTVWMMPVLGAYIADAHLGRYRTLLISSIICLVAMSVLTLAVSIPSLKPPP 134
           A+ NVT W GT ++ P++GA +ADA+ GRY T+   S I  + MS LTL+ S+P+LKP  
Sbjct: 84  AATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE 143

Query: 135 CQEANKDNCKQQATKLQLAVFFGSLYLLAVASGGTKPNISTLGADQFDDFDPKEKAQKLS 194
           C     D C   AT  Q A+FFG LYL+A+ +GG KP +S+ GADQFDD D +E+ +K S
Sbjct: 144 C---IGDFC-PSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKAS 203

Query: 195 FFNWWLFTVFSGILFASTILVYIQDNVGWGLGYGIPTIGVAFAILIFVCGTPFYRHKLIS 254
           FFNW+ F++  G L +S++LV+IQ+N GWGLG+GIPT+ +  AI  F  GTP YR +   
Sbjct: 204 FFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQ--K 263

Query: 255 YKGGPFIRMANVIVAAAWNWSLPLPNDPKELYELEIQHYSKSTTFKIDTTPSLRFLNKAA 314
             G P  R++ V+VA+    S+ +P D   LYE + ++ + + + KI+ T   ++L+KAA
Sbjct: 264 PGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAA 323

Query: 315 IRT-------NASNSWRLCSVTQVEETKQMLRMIPILICTFMPSTMVAQTHTLFIKQGTT 374
           + +       + SNSWRLC+VTQVEE K ++RM PI     + S + AQ  T+F++QG  
Sbjct: 324 VISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRA 383

Query: 375 LDRSIGSHFEVPPASLYAFITISMLVSIVIYDRMFVKIMQKVTKNPRGITMVQRMGLGLI 434
           ++  IGS F++PPA+L  F T S+++ + +YDR  V + +K T   +G T +QRMG+GL 
Sbjct: 384 MNCKIGS-FQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLF 443

Query: 435 CHVLVMTVASRVERHRLEVARTNGLVQNGSGHEQLPLTIFTLLPQFILMGIADALLQIAN 494
             VL M  A+ VE  RL +A   GLV++G+    +P+++   +PQ+ ++G A+    I  
Sbjct: 444 VSVLCMAAAAIVEIIRLHMANDLGLVESGA---PVPISVLWQIPQYFILGAAEVFYFIGQ 503

Query: 495 TEFFYDQAPESMKSLGSSYTLTSLGIGNFLSSFILSTVSNITKKHG-NGWILNNLNASHL 554
            EFFYDQ+P++M+SL S+  L +  +GN+LSS IL+ V+  T ++G  GWI +NLN+ HL
Sbjct: 504 LEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHL 563

Query: 555 DYFYALLAVMSAVNFFLFLVVSHFYEYK 575
           DYF+ LLA +S VN  ++   +  Y+ K
Sbjct: 564 DYFFWLLAGLSLVNMAVYFFSAARYKQK 581

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_031737789.14.8e-27378.97protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Cucumis sativus] >KAE8650326.1 hyp... [more]
XP_038874856.11.2e-27179.17protein NRT1/ PTR FAMILY 5.2-like [Benincasa hispida][more]
XP_022143759.11.5e-26678.00protein NRT1/ PTR FAMILY 5.2-like [Momordica charantia][more]
KAA0049221.11.8e-26477.83protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa][more]
XP_022924431.16.0e-26077.61protein NRT1/ PTR FAMILY 5.2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FNL71.1e-20862.16Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1[more]
Q9FNL83.2e-20360.55Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1[more]
Q8VZR75.3e-15048.85Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2[more]
Q9M3901.0e-13243.78Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
P460328.8e-12944.54Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CQA17.2e-26778.00protein NRT1/ PTR FAMILY 5.2-like OS=Momordica charantia OX=3673 GN=LOC111013596... [more]
A0A5A7U6G88.8e-26577.83Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A6J1E9F42.9e-26077.61protein NRT1/ PTR FAMILY 5.2-like OS=Cucurbita moschata OX=3662 GN=LOC111431936 ... [more]
A0A6J1IQK56.5e-26074.92protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1E9569.4e-25974.75protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G46050.18.0e-21062.16peptide transporter 3 [more]
AT5G46040.12.2e-20460.55Major facilitator superfamily protein [more]
AT2G40460.13.8e-15148.85Major facilitator superfamily protein [more]
AT3G54140.17.1e-13443.78peptide transporter 1 [more]
AT2G02040.16.2e-13044.54peptide transporter 2 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 104..536
e-value: 6.6E-96
score: 321.5
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 19..584
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 7..579
e-value: 5.8E-186
score: 620.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 24..568
NoneNo IPR availablePANTHERPTHR11654:SF453PROTEIN NRT1/ PTR FAMILY 5.2-RELATEDcoord: 19..584
IPR044739NRT1/PTR familyCDDcd17417MFS_NPF5coord: 36..573
e-value: 0.0
score: 637.389

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0000489.1Sed0000489.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0042938 dipeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity