Sed0000326 (gene) Chayote v1

Overview
NameSed0000326
Typegene
OrganismSechium edule (Chayote v1)
Descriptionmalonate--CoA ligase
LocationLG07: 11221807 .. 11235432 (-)
RNA-Seq ExpressionSed0000326
SyntenySed0000326
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCAATAAGCAGCGTTTCTTGGCAGTGGCTATAAAGGAATTGATTTCTTTGATGGCGATTTATCAATCATAATCGCCACTGCCCACAAAATCGAGCTTTTAATTTCGAACCAAATTTTTCAAAACGAAACAGAGCCAAACCCAAAACCCCAAATGGCAAATTTACGAGCCCCACACCATAATTTTGGGGTTTGAATCGCCATAACCAAATATTAAACGCAAGCAATTACGACCCACCGCCCTTTGTCCATTTTTTCTGCTAACCCACCTACCCTTTCTTCATGAACTCTTCTTCATTTAATTATCCCACCTGTATATGTCATCTTTCATTTCCCCCTTCTCATTTGATCGCCATTTCGTCCAGTTCTGCTTGTTTTCGTCTTGGTAGAACCATTTCTAAGCTCTGTTTTCCACGTTCTCGACTTTTTTCAGGTGGGGTTTCTTCCTTTTCTCTTACTTGCTTCTTTTTTATGCATATAAGAGATGTGAAATGATGGGTATTTGTTTTTGTAGTTCTTCATTTAGTTTAAGGGTCTTTTAGAATGTTTCTTTATCTTGTTTGATTTAGAATTGTGTTTGTTGATTGTTCTTATGTGGGTTTGCCTTTTGAATTTGTGCTGTTATTTTAGGACACCTGCTAGTTGTGACTTATTTGTTCTGATATGTATGGGATGCGCTATAATATTTCTTCAATCTAATACCGGTAATTCAGCTATGCAATTCGTCCACTTTGGTTTTGCCACTTACCTAGTGTGGTGGAACCTTAAAAATCTAGTTTATGGTTATAATGATCTTTGTGGTAGAATTGTAGAATGTATAATTGAAGCTTAATCATGACATTGCTACTATTCAATGACTATAAATCTCTTTAAATCTTGAGTCAAGACGATTGATCCATATTGGTTTCCTCACTTACCTAGGTCCTAGTATGGTGGGGCCAGATCTTAGTGTAAGGGGTCATCTTTATGGTTAACTTCCATGTATGCATATCTGAACCTCAATCAAGGCATTGCTGCTCTGAAGTTTATAGTTTGTTACATTATGAAGTCCGGGGAGCTAGTTTTCCAATAAGAGTAATAGCTCAATCGACATAAGTGTGTGCTCATGACCTAAGGCCATGGGTTCAAATCTCCACCTCAATTGTTGTTGAACTCAAAAAACTCCAGGCAGCTAGTTTTGTAATAATCTGACTGAAGTTTCGAAAAATGTGCTTGGGTTCCACCAATGGGAAGTATATAAGTTTGGTGTCAACTGTTTTGCATAACTGTTTTACCTGTTCTTATTCTGGTTCTTATTTGTTTCTCATGTCATGTAGTATTAAGTTCTCCTACCTTCATGGAGGTGGTCAAATCAGCTGCTAAGCACGGGTCGACTTGTCACAAGAATGTCGCTATTAGAGCTAACCAGAAAAGTCACAGTTACATACAACTGATCAAATCTGCACTAAAGATATCTGATCTATTGACTAGAAGTGATTTACGAACGGTATGTAGCCTTTATATAGTGAAAACTGAATATTTTTGTTTGATACTGTGGCCTTTGCTTAGTATACCTGTGTATGAATGAGGTGAACCATTGGTAATTGTTGTCATAAGATTGTAGTAAGCAGATGTTCTAATTGATGTTATAATCTGGATGATAAAATTCTCCCTCATCCTCCTTACTCCAACCTCCTCCCTTTTCTCATTCTCTTGCTTTCAAGTCCACCCCTCGTCAAGGTCTCAAAGTTCCATGATTCATGGAGTTGTTATTCACCATAATTCTCCTGCTGCTTCGCTTGATCTAGTTTCAGACATGGAAGATGATGTTAGTGTGAGCAATATTGAATTGTCCTCAAGCGGAGTTTATTTCTCCTTCTGATTTAGCTGAACTAGCTATGTCCTCTCCATGGCCTCTGACCCTCCGTCTGATGATCAGCAGTGTGATCCAATTGCAATCCCGAAAACGTTTTCCAATATTGTTGCCACTTGTGGATTTCAGTTAAAGGCATGTCGTGGTTTTGCAGTTTGCTCCAGTTGAAGTTTGAAAGTTTCTTTTTGGGTTGAGCTACTCTGTCTTCCTTTTTGGTTTCTGGGTTTGTTGTGTCTCCTCAGTCATATGCTTTCTTTAGTCTCCCTTGGCGTCTATGTTAGTTGTGACATTTTTTCTTTGAAGATCGAAGCTTGTATATTTTTTACTATTTTTTTAAAAATCTTGTATCATTTTTTCATTCTTGGCACTTCAAACTTACTGTTGTATTTTGAACCTGGTGTGGTTCTTGTTTGTCTTGGCTGCTGTTTATTTCTTTAGTCTTGGCTTTGGTCTTTTTGCCTCTTTTCCTTTTGTTTTCCCCCTCTTTTTGAGGTTTGTATCTGAAGTTTTTGCCTCTATTCATAGAAATGTTTGTTTCTTGTTTCAAAAAAAGATCTGGATGATAAAACAACAAGGTGTTGACATGGTTAGGCATTTGACTTCCAAAATGTATTCTTTTTTCTGTTTCGAAGAAAATGTCACCGATGTACTGTTTGCTTCACATAAATCATTGTTTTGGTGCTTGAGACATAACTGTAAGTTCTTGCTGTATTTTCTTTAAGCATGATTGTGTACCCTCTATAGATTTTTGGAACTCTCATGCAATTGGTCTGCCATCTTATTAAATGATGTGAAGGGTCATGGGCATGTTGGTGGAGCTAAAATTGGAATTGTGGCAAAACCATCGGCTGAATTTCTTGCTGGAGTGCTGGGGACTTGGTTTAGTGGAGGTGTTGCTGTTCCACTTGCACTTAGCTATCCAGAGGCTGAGCTCTTACACGTCTTGAATGATTCGGTTTGCTCTTACCTGCCCTCTCATAACTTTTTTCCCTTTAAATTTTGGTATCTGGCTTTTTACTTGCAATATGTTGTGTCAAATATCTCACTTTGTTGATTGTGTAACCTATCATGCATCTGTAGTTTCAGAAGTTCCTACATCCCTTATTTTTCTGGCTCTTGAATCAAGAGTTCATGTTTTTCCTTTATCCTATTACTGGAAAGTTCTACCGTGACTAGTTTCATCCGTTAGCATCTATTTCTAATTTCCTTTTATTTTTATGGGTGGGGAAAGTGTGGATTATTTCGCTATCGCATCTTTTTTAACTTTGTAAATCTATTTAACACTTAACAGTATGCTTGACTATAGGAGGTCTCCATGATACTGAGTACTGAAGATCATGCTGAATCGATGCAAAGTGTTGCTGCTAAAACACTTGCAAAGTTTTCTCTTATTCCACCAGTTCCTCATGATCAATCAACCAGTATAGAAGAAGATGCAGCAGATGCAGAAAGAGTTTTTCAGGGGACAGTAGAGAATTTGAGTAAGATGTTTGAATAGCTTAGCGAAATTATGCATTGCACCTATCAAGCCATGCTTTTTATTTCTCATTTCAAATGTGCAGGTGATGATCCTGCATTGATAATTTATACTAGTGGTACAACTGGTAAACCGAAAGGAGCCATCCACACGCACAAAAGCATCACTTCACAGGTATGACATGGTTTTCTGACATGGTTTTCTATGTGTAATAATAAATTATGAATTTATAATTAACAACCTCGCTGCACATAAGTAATAACAGCAATATATGTGAGGTCCTTATTAGATTCAATGTTTCTTTTTAAGCTTCTATTGCTAGACATTTTTAATTACCCTGTAGGAGTAATTCTATTAGATTTGAGCCCCATATATAGATGGTCCCCTGTTTGTTAGGGCTGTTGATTTGTATGCCCCTCTTTGTATTATTCTTTCATTCTTTCCTTATTGAAAGTTTGGTTCATTATAAATAATACAAAAAATGTGAGGTCCACATTCACAATTTACAGTAATCTTTTTGGATTCTTTGGTCTAATTTCAGGTTCAGACATTGTCAAAAGCTTGGGAATATACATCAGCAGACCAGTTTCTGCATTGTCTTCCCCTGCACCATATCCTTGAAATTTTGTATAATTGTTGCAGTCATTTTAGTCGTCTTATTTCAGCAACTTAAAATTTTAAAATAGTTTGGAAATGTTGTTGATTTTAACTGGTGCTATACATGTGCATGGACTGTTCAATGGCCTGTTGGCCCCTATCTACGCAGGTTCCACGGTAATTTAACTTTAACGAGATTGTTGTTTATGCATAAAAATTCTTGCTTCATTTTGAAGTCAGCTTGACTTTTGTTTATTTTCATGTTTTCAATCAAGGTTGAGTTTATGCCAAAATTTAGTGTCAGTGGGATCTGGCAACGGTGGCGGGAATCATATCCATCCAATGATTCTAAATCCGATGACGCTATAACAGTTTTTACCGGGGTAAGCATAGACTTTATGTTGAGTATACAGTTCTTTTTGTTGCTATACTGCTTCGTTGGCTAAAATATATCACTATACACAAGCGCCATGAAACTTGCACCGTCTCAAATATAGATTTGGTTAAAATAATCGGCTGTCTGATTTTAGGCATTAGATATGTTTTGGTTATTCCTTTTATTTTGCACATTATTCACAATTATTTGAACATTTGATGCATTAATATTCTTGTACCAGAGGTTAAAAATTGCACCTTGGAGCATCATTATTCATATCGAAAAGTTCTAATGTGTTTTTAAGTGTTCTCTGGTCAAATATTTCTGGATGTATAGGTTCCAACCATTTATTCTCGACTGTTACAAGGCTATGAAGCTATGGAACCAGAGCTGCAGGTTGCTTCTGCCTCTGCTGCAAGACAGTTGCGCCTAATGGTGAGTTTGAGGAAAGGATCATGAGATACTAGTTGAATACTATAACGTTGAATAGTTCATTTTTCGAGCCCAATAACAGTATTTTTGGGGGGTTTAAAATAGTAAATGTCTTGTATAGTAGGATTATTGACCGAAGTTCTGCCTTAAAATTTACCCCCATCTTCTTCAGATGTGTGGCTCTTCTGCACTTCCTCCTCCAATCATGAAACAATGGAAGGTGGTAACTGGACACCAACTTTTAGAACGATATGGTATGACCGAAGTAAGTAAATTTGGTTAGACTTTGTCAAGGTTGCATCCTCTTGATACATCGCATTTTATTTCGGTACATTTTACTTTTTGTTGATAGGAAAAGCTCTAAAATATCATTCTCTTGATTTTGTTTGTGTTCTAGTTTGTCATGGCAATTTCAAATCCTTTAAATGGTAACAGAAAGATTGGAACTGTTGGCAAGCCCTTCCCTGGCGTGGAGGTTAGTCTTAATACTTATATTCTGAAAAATGTTCTGGAAAAAAAAAATTCTTGTTCATTTGAATGGTCTCCCTGTATCGTCTTCTTTTTCTATTTCCAACTGTTGTAAGGAATTTTACTCTTACATTATAGATAAGAGAGAAGAACTTCCTAGTAGGATGACCTCCCCATCCAGGAATTGAGCAATGATAAGATGGTCATACCATTGTTACTTTTTTTTTTGTTGTTGTGAGAAACGAGGAGGGAAATTGTTGGTTTATGTTTATTTTAGTATTTTCATCTTCTTAATTATTTGTTCCTTTTAGATTAGATTAAATATTATTTTCTATCTTTTCTTTTTATGACTATTTAGTATCTTTTCTTTTTATTATTAGGTCATCTTTTCCTTTTATGATTAGGTAGTAACTTTTTATTTTATGATTAGGTAGTAACTTTTTATTTTATGATTAGGTTATCTCTTGTAATCTATTTAAACCAAATATTGGGTTCATTATTAAATGAACAATTTGAATTCCGTCTTTCAACATGGTATCAGAGTCTCAAATTTTTCCTTTCAATACATGGTTAAATCTTTTCTCTTCTCCTCCTTCATCTCAAAACCTAGAAAATAATCCTCTCTATTATTCCTCACTTTTTTTTATTCATCGAGAAAATCAACCAAATTTTCTCTCTTCAAAGGTGTCAATGAACTGCCTGCAATTGGAAGAATCATACCGGCACCTGTCCTGACCAATATTCTCAATTGACCCTGACCAATATTCTCGACCCTGACCAGTATTCCCGATTGGCCCTGACAAATATTCCCGATTGGCCATGACCAGTATTATTCCCTATTGACCTTGTACGGTCTTCCCGATTTTCCGTGACATCAGTATTCCCTATTGGCCCTTTTCAGTATTCTCGATTGGCCCCGACAACACTCTTCTCAATTAGCTCTTGGCCCTGGCAGCAGCTATCCCAGTTGGCGAGGCATCATCCTAATATGATCCAAGATCTAGATTATATCAAAAGATAAAAGAATAAAGATAAGCCAAGTTTTGATAGGTCTTGGAACCACCTATTTCAAGAGAATCTCTTGAGCCGTATAATAAATCACTCAATAACTCATTTAATAATTGGATGTCTAAACCCTAACCTAACCCACCCTATTTATAACAAAATCCTAACTTACTCTCTAAGTAATTGCTAACATAGAGCTCTAGGCCCAACCTTATCAATACCCGCCACTTTAAAGCACCTTGTCCTCAAGGTGGGCATACACAAAAATAGAAATAGAAAAACAAATAAATGAAAACAATTGTTTTTTTTTTTTGGCGGCAACAGCAGCGGGGTGTCAGTGGTGGCCGAAACGACATCGTTGGTGGCGGCAGCGGTGGAAACGGCGGCGGTGGCGGCGGCGATGGCGGCAGCAGTGGAGGTGGCGGCGGCGATGTCAGAGGTGGTGGCGGCGGTGGTGTAAAAACCCTAGCCGTCACTTTTTCTTCCACGACCTTCCTTCTTCCTCGAATCCTAGCTTCCATCTCTGAGTACCATTGTTCCCTTGCCTTGGATTGCTCCTCTCTTTCTAATCTTCTTCGTTCCCGCTCCTTTGCACTCTCCTCCCTTATCTCTCTAAAACAACTTTTTATGTCATTGTAAAATTTTTGCCACATCTCTTGAAATAAGTGAACATGTTCTTGATCCATCTCTACAACCAAGAATCGAAACGGCTCTGATACCAATTGATATGATCCAGGATCTAGATTCTATCAAAAGATGAATGATTGCAAGATAAACCAAGATTTGATAGGTCTTGGAACCTCCCCTCTCAAGAGAATCTCTTGAACCGTACAATAAATCACTCAATAATTGGATGCCTAAACCCTAACCTAACCCACCCTATTTATAACAAAATCCTAACTTACTCTCCAAGTAATTGCTAACATAGATCCTTAGGTCCAATCGAGCCCTAGGCCCAATCTTATCACATCCCAATTCACATCATCATCTCAATTGGTGCATCATCATCCCAATTGGCACATCGTCGTCACAATTGGTGCATCATTATTACAATGACCAATTCTTATCAATTTAACTTCAAGCTTGGCTTGTATTTCATATACAATCCAGCTTGAGGGGGAGTGTTAGTTTATGTTTATTTTAGTATTTTCATCTTTTTAATTATTTATTCTTTTTAGAATTAGATTAAATATTATTCTCTATCTTTTTCTTTTATGACTAGGTAGTATCTTTTTCTTTATTATTAGGTCATCTTTTCCTTTTATGATTAGGTAGTATATGTTACTTTTATAATTAGGTTATCTCTTGTAATCTATTTAAACCCAATATTGGGTTCATTGTTAAATGAGCAATTTGAATTCCATCTTCCAACGGAAACTAAATTAATTTAAAGAGAAAGTTCACAAAAACTGAACAATGAGATAAGATATCATCCTAAAAAATGATTTCTCAGTATGTTACAAAATATCTCGTTAGAATTAATCAAAAGAGATCGGTAGTTCCAAGAAGCCCGTAATCTATTTAAACCCAATATTGGGTTCATTGTTAAATGAGCAATTTGAATTCCATCTTCCAACGGAAACTAAATTAATTTAAAGAGAAAGTTCACAAAAACTGAACAATGAGATAAGATATCATCCTAAAAAATGATTTCTCAGTATGTTACAAAATATCTCGTTAGAATTAATCAAAAGAGATCGGTAGTTCCAAGAAGCCCTTGACAAGGATGCTCTTTTTAGTGATAGGAAAATGCCAAATTCTAGGAAGTTGTTAATTTCCTCCCTCCAAATATTTTGAAATTCAAGCCACGAGCACTTATTTATTTCTTACGGACTGGTTAAACAACATAGAAACCAAGAAAGGAAATGTCAGAGTGACACCCATCTGAGGTCATCGGTTCGAATCTCTGTGTAATCTTATAATAAGGTGTTGGTTATTTGATGATTATTCTCAAATTATTTGTTTCCATATTTATTGGATTAGTATCTTGTATTTATAATATTATTGATTTGTAGAGGATATTATCCTAATGTATTATTTGTTTCCTTATTTGTAAAGTAAGGGTTTCTCTATTTAAGAAACACCTATTATCAAAGAATAATATAGAAAAATATTCCTCTCTTTATATTTTGTACATATTAGAGAATATGGGGTTCCACCTCAAAACCAATTGGTAATGAGAGGAGTAACCCATACCCCTTATAAATGTGTTGAAGACTCCTCACCTTTCCAATGTGGGACAATTGCCACATGGTGAGCATAGTGAGTATCTTCTACATGCCCCCTCAAGATGGCGGCCATATCGGGAAATGCCATCTTGAAATGGGTCATAATTTGTGGACCGAAATACCCGCTTTGGCTTACTCGCATAGGTCGGATCCAAACTCTGGGATCAAATACCCGCTTAAGCTTATGCCCGCTTGGGTGGGGTCCACACCCGTTTTTTTTTAATTCAAAGCATTTTGGGTAGGATCGGACTTTGGGGGGACCATTAACCCACATGGAGCTCTGATACCATACTAGATAATATGGGGTTCCACCTCAAAACCAATTGGTAATGAGAGGAGTAACCCATACCCCTTATAAATGTGTTGAAAACTCCACACCTTTCCAATGTGGGACAATTGCACATGGTGAGCATAGTGAGTATCTCCAACATAAGGATAGTCATTAATGTCTCTTAGAGTTACTTTCACCATAAGGGCTAGGAGTGTACAAATGAACCGACAACCCGACGAACCCGGACAACCCAACCCAAATTATAAGGGTTGGGTTGGGTTGAGTTTATTTTCGGTTCGGGTTGGGTCCAAAAAATGAAGAACCGAAATTTTCGGTTCGGGTTCGGGTTCGGGTTCGGGTTACTTATATTTTGGTTCGGTTCAACCCGAACCGAACCGAGATATGTATTAACTATGTGATAATTGTACCAAAAATGTATAGTGGCGTGGTGGTGTTTCTGCTATTATCACTTCCATGGGGCATGGGGTTCGAGCCTGCTTATAGCCAATTTTTATGAATTGTTTTTAAAAGCCTTTTAATAAATACTTTTTCTTTTGTTTTTTTAGAATATCTTTTTTTTTGTAGTTAATTTTTTTGGCAGACATTTATAAATAAAGGTAAGGTATTATTAGTGAGTTTTTAGAATATCTATTTATATAAATATATATATATATATATATATATATATATTTATTAATAACTCCTTGTATTATTTTTTATAGTTAATTTTTGTGATAGACAATTATAAACAAAGGAAATATATTATTAGTCTAAAATATATTTTCAACATCCTCCCTTTTATTTATAATAATAATGTCAATAAGATACTATTTAATGCAAAATTTAATTTATGTATTTTTGTAGCAGCTCAATGACATTAGTGTAGTTTCATGATCAAAATTTCTCTTCTTTCATAATAATTGAACTAAACAAAATGAAATTCAATTAAGTACAAATTGAAAATGTCCATTTTTTTTCTTCACTTTCTTTATGTTTTAATAACATTTATTTCCAATTTAATTTTTTAAAATTTTGATTTATGTACATATCGAAGCTTGAGATGGTAGTTTTTGCTTGATGTTTTCAATACAAATTTATGCATAAAAAAATTATCGATTCGATTTCAATACCAAATATTTTTTTCTCATAATTTTTTTAAACTACAACATATACTAATGTAACCCGAGGAACCCAACCCAACCCAACCCGAAAATATAGGGTTGGGTTGGGGTTGGGTTGTCTTTTTAATTGGGTTGGGTTGGGTTGGAAAAGTGACCAACCCGAAAATTTGGGTTGGGTTGAAAACCCCCTCAACCCAACCCAACCCAACCCATGTTCACCCCTAATAAGGGCCTTGGGATGGACACAAACTTTTAAAAGAAAAATAAAGGCTAAGGTTTTACATCTTGTTATTAAATGTTTGCTCTTATCTTAGACAAAACAGTATTGCAGTCCTATATATATTAAAAGCAAACCAGCGTCATGAACTCAATTTTGTTAATTTTGACTACAAAGATGTAACAAGGGTGTTAAATTAGTGTTGGTTAACCTGTGCTTGCAAAAGATCTTCTAACCGTAGCTAAATATCTACTGCCCTTCATGTATTAGGCCAAGATCGTTGCAGATGATGGGAGTACTGATGATTCAAACTTGGAGGGTGAGCTATGTATTAGAAGTCCTTCAATGTTTAAAGAATATTGGAAGCTTCCCGAGGTACGGATAACCGTACTAATTTTTGGTTAGCATATGCCTAGTTGATTCAAGTATTTTACTGACCAGAATTAGAACAATATTCTGAATGAAACCTTACTTATTGGGTCTTTTTTTTAAAAACAAAAACGGTAGTCTTCTCATATGCGTGAAATGCACAACTAGCAAAGAATTGAGGAATGAACCACCTGAAAGTCGTTTCGATCCAATCCATTCTTGGAGTTAGTGAAGTGACAACTTAACCACTAAATTCAATTAGTTTGAGACTTCAATTCCAGCTTAAAATCTTTGGTTAAATTACAAATTTGGTCCGATTGAGATAAATTTAGAATTTAGTTCTTATGTAGTCCTTGTCATTCGAAATCCTAGACAAATTAAATACGCGTTAGTTCTCTATTCATGTGCCAATGATAACGAGTAAATAAAAACAGCAGGGTACATCCATCTGACAGCTAGGAGTTGTTAACAAAGCATTTTCATAGATGTCTTTGCATGCCCATATCATGTTTGAAAGCATCAACTTGCTGAGCTCTATTCTCTTGTCAGCTAGTCCTCGTGTAGCTTCTTTAGAATTTGGCAGCTCAAGTTTTATCTAATCAACTATTCAACTCACACCGTTAACAAAGTACAAAAGACCACCTCATTTACATATGAATAGCAAAAAGCCGCATACGTAATTTTCCCAAGATTACCCATGGTAATGACTATTTTCTTATGTTTTATCAAATCGAGATTAAATTCTAATTTTTAAAATGATAGAGACTACATTCCAATTTTTTTTCAAACTATAGAGACCAAATATATAATTTAACCAAAATCTTCTCACTTGCCCTCGTAACTTTACAAAAGAGAGGGTAAAGAAAAGAAGACTAGGATTTTAATCGATTCAAATCCTGTGATTTCTATTCTAATTACAAATTCTTTCCGTAGGTTACCAAAGCGTCATTTATTGATGGAGGTTTCTTCAAGACTGGAGATGCTGTTAGACTGGATGAAGATGGTTACTACATCATACTAGGACGTAAGTTACCGATAAAGTACAATTGCTTGTGCTTCCATTGATTAGTGTAGATTTTACGCCCTACTTATTATGTGCTTGCTTCTCTAGTGCTTTGCCAATACATTTCACATCTAGTTCATAGTTCATATACTGGCTTTGCTCTACTGTGTTCTTTTACCTATGCTTGTCAACTCTTTTTGCTTTTATGACCAAGTCTGAGCATATGATGATGCAAAGAATTGATACCTATTAGACAACTAGCATCATCTTCTACCTTGTTGAATAAAACATGCTGTCTTATTCCTTGTCGGGCACGAGTGTTGGCGGGATCCTTTGGCGTTCCATCTTTGCCAGTCCTATAGGTAGGTGTACGTTGGTACATGCATCTGACAGCTAGAAGTTGTTAACAAAGCATTACAGAGATGTCTTTCCATGCTCATATCATGATTGAAAACATTAACTTGCTGAGCTTCATGCTCTTGTCAGCTAGGTGCATGTTTTAGTACATTTTATAAAAACCCTCCTAGTTTGGTGGTATATGCTTCCACACCTCACAAATGACAAATAGGCAATAAAGTTGACATGTAAATTTTGTAAATCTTCATGTGTATCCAGTACAAGGAATTTGGGGACCCTGCATCTCTTCCCTATAGTTGAGTTCAGTCAATAACAAAATAACTTTGAAATACCCCACCACTAACTTATCAGTGTCCCACTGTTCTTCTCTTTGTACTCCTTCCTCACATGACTGTTACTGCATAACTAACTCCTGCCACATCTTTCCCCATCTTTCTTTATTCTTCTAATTTCATATCAACAATGAAGTTGTTTCTACCAAAAAAGGAAAAATGGGTTAGGTATTGCAGCCTCTTATAATACTTGCAACATGTGAACTTTGTGGGTCAATCTCAACATCATCCACCAAAGTTTAGTCGCTTCATTTCCGATCTTATCACTAATTAATTCGACGACAGTCTTCTCTTCTTTTTCTGTTTGTTTTAAACTTGATTTTCTAAAAGTTGTTTTCATTGGGAATACAAACAAAGTAGTTCCACTCCACCTTGGTTAAATAAAAGGTAGATCTAGGGAGGATAAGCATCTCAATTGGTATGAGACAATTTAAGTAAAAGTAAAAACAAAATCACGAGGGCTTATGTCCAAAGTAGACAATATCATACCCAATGTGTAGATCCCTCGAGTGTTGGGTTGTCGTCTCGGCCTATTACAATGAATAGTCAATAATCCATTCTTTTGCTTTGATTTGGTTGATGTTTTCAGGTATGAACGCCGATATCATGAAAGTCGGTGGATTTAAGTTATCGGCATTAGAGATCGAATCAGTGATTTTGCAGGTAACCCATTTCTCCTGTATTCTCTCTCTTTTTCTCAAAAGAAAAGGGAAAGACAACCCTCACACCCACCACAAACAAAAGGAAGAATAAAGAAAACATGAAATGACACCGGCTAAATCACTTTGTGCAGCATCCTTCTGTCATAGAATGTTGTGTGTTGGGATTACCAGACAAAGACTATGGAGAACGTGTCTGTGCAATTATTGTGCTCAACTCTAAAACGACGACGACGCCTGGTGAGTCCAAGCCTACAATGAGCTTAGATGAACTCCGCACATGGGCTAAAGACAAGCTTGCTCCATACAAGGTAATCCATGGAGGAGATTTTAGTTGTTAAAAGTAAAATTGTCATTTCTGTGTAAATACATTATTATTAAGTCAGGGGATTTAATAATATTTTTAATAATACGACGACGCATTCTTTCCAGTTACCGACCATGTTGCTGTTGAAGGACTCGCTGCCTCGAAACGCCATGGGAAAGGTCGGTTGCAATCTTCAACTGGCATTGCTTTGCTTTCTTTCACTGTCTTTGTTTGTACTGAAATTTCTCTTGAACTGCAGGTGAACAAGAAAGAGTTGAAGAATAAACTGGCAGAAGAAGACTAAGCTTTAGTAGTGAACCTATTGCTATTGGTGATCAAAGATGAGGAATAAACATTGATTAGTAGTATCAATATTTGATTATTTGATAGGTTGTAATTTGTGCTGTTAGCAATTAATTAGCTTCAATAATGTTCTATCATTCCTAGTCATCTGAAGTATCAAATTTTTTTTTTTTTAGAAACAAGTTGAAATTTGGAATGAGTTGCAAATTTCACTAAACATTTTTTTTAGAAACACCTAGTCTTAAACATTCTT

mRNA sequence

CTTCAATAAGCAGCGTTTCTTGGCAGTGGCTATAAAGGAATTGATTTCTTTGATGGCGATTTATCAATCATAATCGCCACTGCCCACAAAATCGAGCTTTTAATTTCGAACCAAATTTTTCAAAACGAAACAGAGCCAAACCCAAAACCCCAAATGGCAAATTTACGAGCCCCACACCATAATTTTGGGGTTTGAATCGCCATAACCAAATATTAAACGCAAGCAATTACGACCCACCGCCCTTTGTCCATTTTTTCTGCTAACCCACCTACCCTTTCTTCATGAACTCTTCTTCATTTAATTATCCCACCTGTATATGTCATCTTTCATTTCCCCCTTCTCATTTGATCGCCATTTCGTCCAGTTCTGCTTGTTTTCGTCTTGGTAGAACCATTTCTAAGCTCTGTTTTCCACGTTCTCGACTTTTTTCAGTATTAAGTTCTCCTACCTTCATGGAGGTGGTCAAATCAGCTGCTAAGCACGGGTCGACTTGTCACAAGAATGTCGCTATTAGAGCTAACCAGAAAAGTCACAGTTACATACAACTGATCAAATCTGCACTAAAGATATCTGATCTATTGACTAGAAGTGATTTACGAACGGGTCATGGGCATGTTGGTGGAGCTAAAATTGGAATTGTGGCAAAACCATCGGCTGAATTTCTTGCTGGAGTGCTGGGGACTTGGTTTAGTGGAGGTGTTGCTGTTCCACTTGCACTTAGCTATCCAGAGGCTGAGCTCTTACACGTCTTGAATGATTCGGAGGTCTCCATGATACTGAGTACTGAAGATCATGCTGAATCGATGCAAAGTGTTGCTGCTAAAACACTTGCAAAGTTTTCTCTTATTCCACCAGTTCCTCATGATCAATCAACCAGTATAGAAGAAGATGCAGCAGATGCAGAAAGAGTTTTTCAGGGGACAGTAGAGAATTTGAGTGATGATCCTGCATTGATAATTTATACTAGTGGTACAACTGGTAAACCGAAAGGAGCCATCCACACGCACAAAAGCATCACTTCACAGGTTCAGACATTGTCAAAAGCTTGGGAATATACATCAGCAGACCAGTTTCTGCATTGTCTTCCCCTGCACCATGTGCATGGACTGTTCAATGGCCTGTTGGCCCCTATCTACGCAGGTTCCACGGTTGAGTTTATGCCAAAATTTAGTGTCAGTGGGATCTGGCAACGGTGGCGGGAATCATATCCATCCAATGATTCTAAATCCGATGACGCTATAACAGTTTTTACCGGGGTTCCAACCATTTATTCTCGACTGTTACAAGGCTATGAAGCTATGGAACCAGAGCTGCAGGTTGCTTCTGCCTCTGCTGCAAGACAGTTGCGCCTAATGATGTGTGGCTCTTCTGCACTTCCTCCTCCAATCATGAAACAATGGAAGGTGGTAACTGGACACCAACTTTTAGAACGATATGGTATGACCGAATTTGTCATGGCAATTTCAAATCCTTTAAATGGTAACAGAAAGATTGGAACTGTTGGCAAGCCCTTCCCTGGCGTGGAGGCCAAGATCGTTGCAGATGATGGGAGTACTGATGATTCAAACTTGGAGGGTGAGCTATGTATTAGAAGTCCTTCAATGTTTAAAGAATATTGGAAGCTTCCCGAGGTTACCAAAGCGTCATTTATTGATGGAGGTTTCTTCAAGACTGGAGATGCTGTTAGACTGGATGAAGATGGTTACTACATCATACTAGGACGTATGAACGCCGATATCATGAAAGTCGGTGGATTTAAGTTATCGGCATTAGAGATCGAATCAGTGATTTTGCAGCATCCTTCTGTCATAGAATGTTGTGTGTTGGGATTACCAGACAAAGACTATGGAGAACGTGTCTGTGCAATTATTGTGCTCAACTCTAAAACGACGACGACGCCTGGTGAGTCCAAGCCTACAATGAGCTTAGATGAACTCCGCACATGGGCTAAAGACAAGCTTGCTCCATACAAGTTACCGACCATGTTGCTGTTGAAGGACTCGCTGCCTCGAAACGCCATGGGAAAGGTGAACAAGAAAGAGTTGAAGAATAAACTGGCAGAAGAAGACTAAGCTTTAGTAGTGAACCTATTGCTATTGGTGATCAAAGATGAGGAATAAACATTGATTAGTAGTATCAATATTTGATTATTTGATAGGTTGTAATTTGTGCTGTTAGCAATTAATTAGCTTCAATAATGTTCTATCATTCCTAGTCATCTGAAGTATCAAATTTTTTTTTTTTTAGAAACAAGTTGAAATTTGGAATGAGTTGCAAATTTCACTAAACATTTTTTTTAGAAACACCTAGTCTTAAACATTCTT

Coding sequence (CDS)

ATGAACTCTTCTTCATTTAATTATCCCACCTGTATATGTCATCTTTCATTTCCCCCTTCTCATTTGATCGCCATTTCGTCCAGTTCTGCTTGTTTTCGTCTTGGTAGAACCATTTCTAAGCTCTGTTTTCCACGTTCTCGACTTTTTTCAGTATTAAGTTCTCCTACCTTCATGGAGGTGGTCAAATCAGCTGCTAAGCACGGGTCGACTTGTCACAAGAATGTCGCTATTAGAGCTAACCAGAAAAGTCACAGTTACATACAACTGATCAAATCTGCACTAAAGATATCTGATCTATTGACTAGAAGTGATTTACGAACGGGTCATGGGCATGTTGGTGGAGCTAAAATTGGAATTGTGGCAAAACCATCGGCTGAATTTCTTGCTGGAGTGCTGGGGACTTGGTTTAGTGGAGGTGTTGCTGTTCCACTTGCACTTAGCTATCCAGAGGCTGAGCTCTTACACGTCTTGAATGATTCGGAGGTCTCCATGATACTGAGTACTGAAGATCATGCTGAATCGATGCAAAGTGTTGCTGCTAAAACACTTGCAAAGTTTTCTCTTATTCCACCAGTTCCTCATGATCAATCAACCAGTATAGAAGAAGATGCAGCAGATGCAGAAAGAGTTTTTCAGGGGACAGTAGAGAATTTGAGTGATGATCCTGCATTGATAATTTATACTAGTGGTACAACTGGTAAACCGAAAGGAGCCATCCACACGCACAAAAGCATCACTTCACAGGTTCAGACATTGTCAAAAGCTTGGGAATATACATCAGCAGACCAGTTTCTGCATTGTCTTCCCCTGCACCATGTGCATGGACTGTTCAATGGCCTGTTGGCCCCTATCTACGCAGGTTCCACGGTTGAGTTTATGCCAAAATTTAGTGTCAGTGGGATCTGGCAACGGTGGCGGGAATCATATCCATCCAATGATTCTAAATCCGATGACGCTATAACAGTTTTTACCGGGGTTCCAACCATTTATTCTCGACTGTTACAAGGCTATGAAGCTATGGAACCAGAGCTGCAGGTTGCTTCTGCCTCTGCTGCAAGACAGTTGCGCCTAATGATGTGTGGCTCTTCTGCACTTCCTCCTCCAATCATGAAACAATGGAAGGTGGTAACTGGACACCAACTTTTAGAACGATATGGTATGACCGAATTTGTCATGGCAATTTCAAATCCTTTAAATGGTAACAGAAAGATTGGAACTGTTGGCAAGCCCTTCCCTGGCGTGGAGGCCAAGATCGTTGCAGATGATGGGAGTACTGATGATTCAAACTTGGAGGGTGAGCTATGTATTAGAAGTCCTTCAATGTTTAAAGAATATTGGAAGCTTCCCGAGGTTACCAAAGCGTCATTTATTGATGGAGGTTTCTTCAAGACTGGAGATGCTGTTAGACTGGATGAAGATGGTTACTACATCATACTAGGACGTATGAACGCCGATATCATGAAAGTCGGTGGATTTAAGTTATCGGCATTAGAGATCGAATCAGTGATTTTGCAGCATCCTTCTGTCATAGAATGTTGTGTGTTGGGATTACCAGACAAAGACTATGGAGAACGTGTCTGTGCAATTATTGTGCTCAACTCTAAAACGACGACGACGCCTGGTGAGTCCAAGCCTACAATGAGCTTAGATGAACTCCGCACATGGGCTAAAGACAAGCTTGCTCCATACAAGTTACCGACCATGTTGCTGTTGAAGGACTCGCTGCCTCGAAACGCCATGGGAAAGGTGAACAAGAAAGAGTTGAAGAATAAACTGGCAGAAGAAGACTAA

Protein sequence

MNSSSFNYPTCICHLSFPPSHLIAISSSSACFRLGRTISKLCFPRSRLFSVLSSPTFMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRTGHGHVGGAKIGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHAESMQSVAAKTLAKFSLIPPVPHDQSTSIEEDAADAERVFQGTVENLSDDPALIIYTSGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEAMEPELQVASASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPLNGNRKIGTVGKPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAVRLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLNSKTTTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKELKNKLAEED
Homology
BLAST of Sed0000326 vs. NCBI nr
Match: XP_038904615.1 (malonate--CoA ligase isoform X1 [Benincasa hispida])

HSP 1 Score: 1048.9 bits (2711), Expect = 1.7e-302
Identity = 534/609 (87.68%), Postives = 556/609 (91.30%), Query Frame = 0

Query: 1   MNSSSFNYPTC----ICHLSFPPSHLIAISSSSACFRLGRTISKLCFPRSRLFSVLSSPT 60
           MNSS+FN  T     ICHLS PPSHLIAI S+SACF LGR   +LCF R RLFSVLSS T
Sbjct: 1   MNSSTFNNLTSLFTRICHLSLPPSHLIAIRSTSACFHLGRNTPQLCFSRFRLFSVLSSYT 60

Query: 61  FMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRTGHGHVGGAK 120
           FMEVVKSAAKHGSTC +NVAIRANQKSHSYIQLI SALKISDLLT S+LRTGH H+GGAK
Sbjct: 61  FMEVVKSAAKHGSTCRENVAIRANQKSHSYIQLISSALKISDLLTSSNLRTGHAHLGGAK 120

Query: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHAESMQ 180
           IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDH E MQ
Sbjct: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHVEFMQ 180

Query: 181 SVAAKTLAKFSLIPPVP--------HDQSTSIEEDAADAERVFQGTVENLSDDPALIIYT 240
           SVAAKT+A  SLIPPVP        HDQST IE DAADA+RVFQGTVENLSDDPALIIYT
Sbjct: 181 SVAAKTVATVSLIPPVPHIYSERNSHDQSTDIEVDAADADRVFQGTVENLSDDPALIIYT 240

Query: 241 SGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS 300
           SGTTGKPKG +HTHKSITSQV TLS AWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVHTLSNAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS 300

Query: 301 TVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEAMEPELQVAS 360
           TVEFMPKFSVSGIW+RWRESYPS  SKSDDAITVFTGVPT+Y+RLLQGYEAM+ ELQ+AS
Sbjct: 301 TVEFMPKFSVSGIWKRWRESYPSKSSKSDDAITVFTGVPTMYARLLQGYEAMDSELQLAS 360

Query: 361 ASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPLNGNRKIGTVG 420
           ASAARQLRLMMCGSSALPPPIMKQWK VTGH+LLERYGMTEFVMAISNPLNG RKIGTVG
Sbjct: 361 ASAARQLRLMMCGSSALPPPIMKQWKEVTGHELLERYGMTEFVMAISNPLNGQRKIGTVG 420

Query: 421 KPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAV 480
           KPFPGVEAKIVADDGST+DS+LEGELCIRSPSMFKEYWKLPEVTK SFIDGGFFKTGDA 
Sbjct: 421 KPFPGVEAKIVADDGSTNDSSLEGELCIRSPSMFKEYWKLPEVTKESFIDGGFFKTGDAA 480

Query: 481 RLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540
           RLDEDGYYIILGR++ADIMKVGG+KLSALEIESVILQHPSV+ECCVLGLPDKDYGERVCA
Sbjct: 481 RLDEDGYYIILGRLSADIMKVGGYKLSALEIESVILQHPSVVECCVLGLPDKDYGERVCA 540

Query: 541 IIVLNSKT-TTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKE 597
           IIVL   T TTTP ES PTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKK+
Sbjct: 541 IIVLQPNTKTTTPDESNPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKD 600

BLAST of Sed0000326 vs. NCBI nr
Match: XP_008452304.1 (PREDICTED: malonate--CoA ligase isoform X1 [Cucumis melo])

HSP 1 Score: 1037.3 bits (2681), Expect = 5.0e-299
Identity = 527/609 (86.54%), Postives = 556/609 (91.30%), Query Frame = 0

Query: 1   MNSSSFNYPTC----ICHLSFPPSHLIAISSSSACFRLGRTISKLCFPRSRLFSVLSSPT 60
           MNSS FN PT     ICHLS P SHLIAI SSSACF +G++I K CF RSRLFSVLSS T
Sbjct: 1   MNSSPFNNPTSLFTRICHLSLPSSHLIAIRSSSACFCIGKSIPKFCFSRSRLFSVLSSYT 60

Query: 61  FMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRTGHGHVGGAK 120
           FMEVVKSAAKHG+TC ++VAIRANQKSHSYIQLI SALKISDLLT SDLRTGHGH+GGAK
Sbjct: 61  FMEVVKSAAKHGATCRESVAIRANQKSHSYIQLISSALKISDLLTSSDLRTGHGHLGGAK 120

Query: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHAESMQ 180
           IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDH E MQ
Sbjct: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHVEFMQ 180

Query: 181 SVAAKTLAKFSLIPPVP--------HDQSTSIEEDAADAERVFQGTVENLSDDPALIIYT 240
           SVAAKT+A  SLIPPVP        HDQST+IE +AADA+RVFQGTVENLSDDPALIIYT
Sbjct: 181 SVAAKTVAIVSLIPPVPHIYSERNSHDQSTNIEVNAADADRVFQGTVENLSDDPALIIYT 240

Query: 241 SGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS 300
           SGTTGKPKG +HTHKSITSQVQTLS AWEYTSADQFLHCLPLHHVHG+FNGLLAPIYAGS
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQTLSNAWEYTSADQFLHCLPLHHVHGMFNGLLAPIYAGS 300

Query: 301 TVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEAMEPELQVAS 360
           TVEFMPKFSVSGIW+RWRESYPS  SKSDDAITVFTGVPT+Y+RLLQGYEAM+ ELQ+AS
Sbjct: 301 TVEFMPKFSVSGIWKRWRESYPSKSSKSDDAITVFTGVPTMYARLLQGYEAMDSELQLAS 360

Query: 361 ASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPLNGNRKIGTVG 420
           ASAARQLRLMMCGSSALPPPIMKQW+ VTGH+LLERYGMTEFVMAISNPLNG RKIGTVG
Sbjct: 361 ASAARQLRLMMCGSSALPPPIMKQWREVTGHELLERYGMTEFVMAISNPLNGQRKIGTVG 420

Query: 421 KPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAV 480
           KPFPGVEAKIVADDGST  S+LEGELCIRSPSMFKEYWKLP+VTK SFIDGGFFKTGDA 
Sbjct: 421 KPFPGVEAKIVADDGSTTGSSLEGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAA 480

Query: 481 RLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540
           RLDEDGYYIILGR++ADIMKVGG+KLSALEIESVILQHPSV ECCVLGLPDKDYGERVCA
Sbjct: 481 RLDEDGYYIILGRLSADIMKVGGYKLSALEIESVILQHPSVTECCVLGLPDKDYGERVCA 540

Query: 541 IIVLNSKT-TTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKE 597
           I+VL + T TT P  S P+MSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKE
Sbjct: 541 IVVLQANTKTTIPDGSNPSMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKE 600

BLAST of Sed0000326 vs. NCBI nr
Match: XP_023515293.1 (malonate--CoA ligase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1035.4 bits (2676), Expect = 1.9e-298
Identity = 527/609 (86.54%), Postives = 552/609 (90.64%), Query Frame = 0

Query: 1   MNSSSFNYPTC----ICHLSFPPSHLIAISSSSACFRLGRTISKLCFPRSRLFSVLSSPT 60
           MNS +FNY T     ICHLSF PSHLIAI S SAC RL R+I K+CFPRSRLFSV SS T
Sbjct: 1   MNSFTFNYSTYLFRRICHLSFAPSHLIAIQSCSACLRLHRSIPKICFPRSRLFSVSSSST 60

Query: 61  FMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRTGHGHVGGAK 120
           FMEVVKSAAKHGSTC +NVAIRAN KSHSY+QLI SALKISDL T  DLRTGHGH+ GAK
Sbjct: 61  FMEVVKSAAKHGSTCRENVAIRANMKSHSYMQLISSALKISDLFTSCDLRTGHGHLDGAK 120

Query: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHAESMQ 180
           IGI+AKPSAEFLAG+LGTWFSGGVAVPLAL+YPE ELLHVLNDSEVSMILSTEDHAE MQ
Sbjct: 121 IGIIAKPSAEFLAGLLGTWFSGGVAVPLALNYPEPELLHVLNDSEVSMILSTEDHAEIMQ 180

Query: 181 SVAAKTLAKFSLIPPVPH--------DQSTSIEEDAADAERVFQGTVENLSDDPALIIYT 240
           SVAAKT AKFSLIPPVPH        DQST IE    DAERVFQGTVENLSDDPAL+IYT
Sbjct: 181 SVAAKTAAKFSLIPPVPHIYSETNSRDQSTDIE---MDAERVFQGTVENLSDDPALVIYT 240

Query: 241 SGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS 300
           SGTTGKPKG +HTHKSITSQVQTLSKAWEYTSADQ LHCLPLHHVHGLFNGLLAPIYAGS
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQLLHCLPLHHVHGLFNGLLAPIYAGS 300

Query: 301 TVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEAMEPELQVAS 360
           TVEFMPKFSVSGIW+RWRESYPSN SK+DDAIT+FTGVPT+Y+RLLQGYEAMEP+LQVAS
Sbjct: 301 TVEFMPKFSVSGIWKRWRESYPSNGSKNDDAITIFTGVPTMYARLLQGYEAMEPDLQVAS 360

Query: 361 ASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPLNGNRKIGTVG 420
           A+AARQLRLMMCGSSALPPPIMKQWK VTGHQLLERYGMTEFVMAISNPLNG RKIGTVG
Sbjct: 361 ANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMTEFVMAISNPLNGERKIGTVG 420

Query: 421 KPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAV 480
           KP PGVEAKIV+DDGS +D+NLEGELCIRSPSMFKEYWKLP+VTK SFIDGGFFKTGDA 
Sbjct: 421 KPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAA 480

Query: 481 RLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540
           RLDEDGYY ILGRMNADIMKVGG+KLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA
Sbjct: 481 RLDEDGYYTILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540

Query: 541 IIVL--NSKTTTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKK 596
           IIVL  NSK  T P +SKP MSLD+LRTWAKDKLAPYKLPT LLLKDSLPRNAMGKVNKK
Sbjct: 541 IIVLHPNSK-ATPPDQSKPNMSLDDLRTWAKDKLAPYKLPTELLLKDSLPRNAMGKVNKK 600

BLAST of Sed0000326 vs. NCBI nr
Match: XP_022964295.1 (malonate--CoA ligase [Cucurbita moschata])

HSP 1 Score: 1030.8 bits (2664), Expect = 4.7e-297
Identity = 523/609 (85.88%), Postives = 551/609 (90.48%), Query Frame = 0

Query: 1   MNSSSFNYPTC----ICHLSFPPSHLIAISSSSACFRLGRTISKLCFPRSRLFSVLSSPT 60
           MNS +FNY T     ICHLSF PSHLIAI S S C RL R+I KLCFPRSRLFSV SS T
Sbjct: 1   MNSFTFNYSTYLFRHICHLSFAPSHLIAIQSCSTCLRLHRSIPKLCFPRSRLFSVSSSST 60

Query: 61  FMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRTGHGHVGGAK 120
           FMEVVKSAA HGSTCH+NVAIRAN KSHSY+QLI SALKISDL T  DLRTGHGH+ GAK
Sbjct: 61  FMEVVKSAANHGSTCHENVAIRANMKSHSYMQLISSALKISDLFTSCDLRTGHGHLDGAK 120

Query: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHAESMQ 180
           IGI+AKPSAEFLAG+LGTWFSGGVAVPLAL+YPE ELLHVLNDSEVSMILSTEDHAE MQ
Sbjct: 121 IGIIAKPSAEFLAGLLGTWFSGGVAVPLALNYPEPELLHVLNDSEVSMILSTEDHAEIMQ 180

Query: 181 SVAAKTLAKFSLIPPVPH--------DQSTSIEEDAADAERVFQGTVENLSDDPALIIYT 240
           SVAAKT AKFSLIPPVPH        DQST IE    DA+RVFQGTVEN SDDPAL+IYT
Sbjct: 181 SVAAKTAAKFSLIPPVPHIYSEINSRDQSTDIE---MDAQRVFQGTVENSSDDPALVIYT 240

Query: 241 SGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS 300
           SGTTGKPKG +HTHKSITSQVQTLSKAWEYTSADQ LHCLPLHHVHGLFNGLLAPIYAGS
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQLLHCLPLHHVHGLFNGLLAPIYAGS 300

Query: 301 TVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEAMEPELQVAS 360
           TVEFMPKFSVSGIW+RWRESYPSN SK+DDAIT+FTGVPT+Y+RLLQGYEAMEP+LQVAS
Sbjct: 301 TVEFMPKFSVSGIWKRWRESYPSNGSKNDDAITIFTGVPTMYARLLQGYEAMEPDLQVAS 360

Query: 361 ASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPLNGNRKIGTVG 420
           A+AARQLRLMMCGSSALPPPIMKQWK VTGHQLLERYGMTEFVMAISNPLNG RKIGTVG
Sbjct: 361 ANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMTEFVMAISNPLNGERKIGTVG 420

Query: 421 KPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAV 480
           KP PGVEAKIV+DDGS +D+NLEGELCIRSPSMFKEYWKLP+VTK SFIDGGFFKTGDA 
Sbjct: 421 KPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAA 480

Query: 481 RLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540
           RLDEDGYY ILGRM+ADIMKVGG+KLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA
Sbjct: 481 RLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540

Query: 541 IIVL--NSKTTTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKK 596
           IIVL  NSK  T P +SKP MSLD+LRTWAKDKLAPYK+PT+LLLKDSLPRNAMGKVNKK
Sbjct: 541 IIVLHPNSK-ATPPDQSKPNMSLDDLRTWAKDKLAPYKIPTVLLLKDSLPRNAMGKVNKK 600

BLAST of Sed0000326 vs. NCBI nr
Match: XP_023000346.1 (malonate--CoA ligase [Cucurbita maxima])

HSP 1 Score: 1030.8 bits (2664), Expect = 4.7e-297
Identity = 526/609 (86.37%), Postives = 551/609 (90.48%), Query Frame = 0

Query: 1   MNSSSFNYPTC----ICHLSFPPSHLIAISSSSACFRLGRTISKLCFPRSRLFSVLSSPT 60
           MNS +FNY T     I HLSF PSHLIAI S SAC RL R+I KLCFPRSRLFSV SS T
Sbjct: 1   MNSFTFNYSTYLFSRIYHLSFAPSHLIAIQSCSACIRLHRSIPKLCFPRSRLFSVSSSST 60

Query: 61  FMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRTGHGHVGGAK 120
           FMEVVKSAAKHGSTC +NVAIRAN KSHSY+QLI SALKISDLLT  DLRTGHGH+GGAK
Sbjct: 61  FMEVVKSAAKHGSTCRENVAIRANMKSHSYMQLISSALKISDLLTSCDLRTGHGHLGGAK 120

Query: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHAESMQ 180
           IGI+AKPSAEFLAG+LGTWFSGGVAVPLAL+YPE ELLHVLNDSEVSMILSTEDHAE MQ
Sbjct: 121 IGIIAKPSAEFLAGLLGTWFSGGVAVPLALNYPEPELLHVLNDSEVSMILSTEDHAEIMQ 180

Query: 181 SVAAKTLAKFSLIPPVPH--------DQSTSIEEDAADAERVFQGTVENLSDDPALIIYT 240
           SVAAKT AKFSLIPPVPH        DQST IE    DAERVFQGTVENLSDDPAL+IYT
Sbjct: 181 SVAAKTAAKFSLIPPVPHIYSETNSRDQSTDIE---MDAERVFQGTVENLSDDPALVIYT 240

Query: 241 SGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS 300
           SGTTGKPKG +HTHKSITSQVQTLSKAWEYTSADQ LHCLPLHHVHGLFNG LAPIYAGS
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQLLHCLPLHHVHGLFNGFLAPIYAGS 300

Query: 301 TVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEAMEPELQVAS 360
           TVEFMPKFSVSGIW+RWRESYPSN SK+DDAIT+FTGVPT+Y+RLLQGYEAMEP+LQV S
Sbjct: 301 TVEFMPKFSVSGIWKRWRESYPSNGSKNDDAITIFTGVPTMYARLLQGYEAMEPDLQVDS 360

Query: 361 ASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPLNGNRKIGTVG 420
           ASAARQLRLMMCGSSALP PIMKQWK VTGHQLLERYGMTEFVMAISNPLNG RKIGTVG
Sbjct: 361 ASAARQLRLMMCGSSALPTPIMKQWKAVTGHQLLERYGMTEFVMAISNPLNGERKIGTVG 420

Query: 421 KPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAV 480
           KP PGVEAKIV+DDGS +D+NLEGELCIRSPSMFKEYWKLP+VTK SFIDGGFFKTGDA 
Sbjct: 421 KPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAA 480

Query: 481 RLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540
           RLDEDGYY ILGRMNADIMKVGG+KLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA
Sbjct: 481 RLDEDGYYTILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540

Query: 541 IIVL--NSKTTTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKK 596
           IIVL  NSK  T P +SKP MSLD+LRTWAKDKLAPYK+PT+LLLKDSLPRNAMGKVNKK
Sbjct: 541 IIVLHPNSK-ATPPDQSKPNMSLDDLRTWAKDKLAPYKIPTVLLLKDSLPRNAMGKVNKK 600

BLAST of Sed0000326 vs. ExPASy Swiss-Prot
Match: M4IS90 (Probable CoA ligase CCL8 OS=Humulus lupulus OX=3486 GN=CCL8 PE=2 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 3.0e-222
Identity = 394/580 (67.93%), Postives = 462/580 (79.66%), Query Frame = 0

Query: 42  CFPRSRLFSVLSSPTFMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLT 101
           C P S L S  ++   ME++K  AK GS+    VAIRA+QKS+SY QLI SA +I  LL 
Sbjct: 29  CRPFSSLHSGSANLILMELLKEVAKEGSSASVGVAIRADQKSYSYKQLISSAQRICSLLC 88

Query: 102 RSDLR-----------------TGHGHVGGAKIGIVAKPSAEFLAGVLGTWFSGGVAVPL 161
            +DL+                 +GHGH+GGA++GIVAKPSAEF+ GVLGTW SGGVAVPL
Sbjct: 89  STDLKASHKAGKQVTPALINGLSGHGHLGGARVGIVAKPSAEFVTGVLGTWLSGGVAVPL 148

Query: 162 ALSYPEAELLHVLNDSEVSMILSTEDHAESMQSVAAKTLAKFSLIPPVPHDQSTSIEEDA 221
           ALSYPEAELLHV+ DS++SMILSTEDH E MQ +AAKT A+FSLIPPVP   S+  +E A
Sbjct: 149 ALSYPEAELLHVMTDSDISMILSTEDHQELMQKIAAKTAAQFSLIPPVP---SSCSQEGA 208

Query: 222 AD--------AERVFQGTVENLSDDPALIIYTSGTTGKPKGAIHTHKSITSQVQTLSKAW 281
            D         + +   T E  +++PALI+YTSGTTGKPKG +HTHKSI +QVQTL+KAW
Sbjct: 209 VDHLQTGDINTDSILHNT-EISNENPALIVYTSGTTGKPKGVVHTHKSINAQVQTLAKAW 268

Query: 282 EYTSADQFLHCLPLHHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWQRWRESYPSNDSKS 341
           EYT ADQFLHCLPLHHVHGLFN L AP+YA STVEFMPKFSV GIWQRWRESYP+ ++K 
Sbjct: 269 EYTPADQFLHCLPLHHVHGLFNALFAPLYARSTVEFMPKFSVRGIWQRWRESYPTCETKV 328

Query: 342 DDAITVFTGVPTIYSRLLQGYEAMEPELQVASASAARQLRLMMCGSSALPPPIMKQWKVV 401
           DD ITVFTGVPT+Y+RL+QGYEAM+PEL+ ASASAA+QLRLMMCGSSALP P+M+QW+ +
Sbjct: 329 DDCITVFTGVPTMYTRLIQGYEAMDPELKEASASAAKQLRLMMCGSSALPIPVMQQWQTI 388

Query: 402 TGHQLLERYGMTEFVMAISNPLNGNRKIGTVGKPFPGVEAKIVADDGSTDDSNLEGELCI 461
           TGH+LLERYGMTEFVMAISNPL G RK GTVGKPFPGVE +IVA+D +  D+   GELC+
Sbjct: 389 TGHRLLERYGMTEFVMAISNPLKGERKAGTVGKPFPGVEVRIVAEDENGSDTTGVGELCV 448

Query: 462 RSPSMFKEYWKLPEVTKASFIDGGFFKTGDAVRLDEDGYYIILGRMNADIMKVGGFKLSA 521
           RSPS+FKEYW+LPEVTK+SF D GFFKTGDA ++DEDGYY+ILGR +ADIMKVGG+KLSA
Sbjct: 449 RSPSLFKEYWRLPEVTKSSFTDDGFFKTGDAGKVDEDGYYVILGRTSADIMKVGGYKLSA 508

Query: 522 LEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLNSKTTTT-PGESKPTMSLDELRTW 581
           LEIESV+L+HP+V ECCVLGLPDKDYGE V AIIV  ++       ESKP +SL+EL +W
Sbjct: 509 LEIESVLLEHPTVEECCVLGLPDKDYGEAVSAIIVPAAEAKKKGEEESKPAISLEELFSW 568

Query: 582 AKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKELKNKLAEE 596
           A+ KLAPYKLPT L L DSLPRNAMGKVNKKELK KL  E
Sbjct: 569 AQHKLAPYKLPTRLFLWDSLPRNAMGKVNKKELKKKLTVE 604

BLAST of Sed0000326 vs. ExPASy Swiss-Prot
Match: Q8H151 (Malonate--CoA ligase OS=Arabidopsis thaliana OX=3702 GN=AAE13 PE=1 SV=2)

HSP 1 Score: 722.6 bits (1864), Expect = 3.6e-207
Identity = 382/619 (61.71%), Postives = 464/619 (74.96%), Query Frame = 0

Query: 5   SFNYPTCICH---------LS---FPPSHLIAISSSSACFRLGRTISKLCFPRSRLFSVL 64
           SFNY + I H         LS    P SH  + S S++ FR         F  + LFS  
Sbjct: 9   SFNYLSLINHRLNHNSYAILSSPLLPRSHPASTSFSNSGFRF--------FQSNHLFS-S 68

Query: 65  SSPTFMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRT----- 124
            S + MEV K+A    S     +AI+A+ KS+SY QL  SAL+IS L  + D        
Sbjct: 69  QSGSLMEVFKAAFSEASNSCDRIAIKADGKSYSYGQLTSSALRISKLFLKDDTTNGGQET 128

Query: 125 ----GHGHVGGAKIGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVS 184
               G G + GA+IGIVAKPSAEF+AGVLGTWFSGGVAVPLALSYPEAELLHV+NDS++S
Sbjct: 129 KKYEGFGSLKGARIGIVAKPSAEFVAGVLGTWFSGGVAVPLALSYPEAELLHVMNDSDIS 188

Query: 185 MILSTEDHAESMQSVAAKTLAKFSLIPPVPHDQSTSI-----EEDAADAERVFQGTVENL 244
           ++LSTEDH+E+M+++AAK+ A+F LIPPV +  S ++     ++D+ +AE  F       
Sbjct: 189 LLLSTEDHSETMKTIAAKSGARFHLIPPVVNSTSETVACNQFQDDSFEAEGKF------- 248

Query: 245 SDDPALIIYTSGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFN 304
            DDPALI+YTSGTTGKPKG +HTH SI SQV+ L++AWEYTSAD FLHCLPLHHVHGLFN
Sbjct: 249 LDDPALIVYTSGTTGKPKGVVHTHNSINSQVRMLTEAWEYTSADHFLHCLPLHHVHGLFN 308

Query: 305 GLLAPIYAGSTVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYE 364
            L AP+YA S VEF+PKFSVSGIW+RWRESYP ND K++D+ITVFTGVPT+Y+RL+QGYE
Sbjct: 309 ALFAPLYARSLVEFLPKFSVSGIWRRWRESYPVNDEKTNDSITVFTGVPTMYTRLIQGYE 368

Query: 365 AMEPELQVASASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPL 424
           AM+ E+Q +SA AAR+LRLMM GSSALP P+M QW+ +TGH+LLERYGMTEFVMA+SNPL
Sbjct: 369 AMDKEMQDSSAFAARKLRLMMSGSSALPRPVMHQWESITGHRLLERYGMTEFVMAMSNPL 428

Query: 425 NGNRKIGTVGKPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVTKASFID 484
            G R  GTVGKP PGVEAKI  D+   +D+N  GE+C++SPS+FKEYW LPEVTK SF +
Sbjct: 429 RGARNAGTVGKPLPGVEAKIKEDE---NDANGVGEICVKSPSLFKEYWNLPEVTKESFTE 488

Query: 485 GGFFKTGDAVRLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLP 544
            G+FKTGDA R+DEDGYY+ILGR +ADIMKVGG+KLSALEIES +L+HP+V ECCVLGL 
Sbjct: 489 DGYFKTGDAGRVDEDGYYVILGRNSADIMKVGGYKLSALEIESTLLEHPTVAECCVLGLT 548

Query: 545 DKDYGERVCAIIVLNSKT-TTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPR 597
           D DYGE V AII+  S        ESKP ++L+EL  WAKDKLAPYKLPT LL+ +SLPR
Sbjct: 549 DNDYGEAVTAIIIAESAAKKRREDESKPVITLEELCGWAKDKLAPYKLPTRLLIWESLPR 608

BLAST of Sed0000326 vs. ExPASy Swiss-Prot
Match: Q58DN7 (Malonate--CoA ligase ACSF3, mitochondrial OS=Bos taurus OX=9913 GN=ACSF3 PE=2 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.3e-103
Identity = 216/564 (38.30%), Postives = 315/564 (55.85%), Query Frame = 0

Query: 52  LSSPTFMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRTGHGH 111
           + +P F + +    +HG   +K++ +R+ + S    QL   A                G 
Sbjct: 49  IRAPAFGDRLALIDQHGRHTYKDLYLRSLRLSREICQLRACA---------------DGD 108

Query: 112 VGGAKIGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDH 171
           +   ++ ++      F+      W SGGVAVPL   +P A+L + + DS  S++L+  +H
Sbjct: 109 LREERVSLLCSNDVSFVVAQWAAWMSGGVAVPLYRKHPRAQLEYFIQDSRSSVVLAGPEH 168

Query: 172 AESMQSVAAKTLAKFSLIPPVPHDQSTSIEEDAADAERVFQGTVENLSDDPALIIYTSGT 231
            E +  VA K       +PP  +       E+    ER       N  D  A+IIYTSGT
Sbjct: 169 VELLSPVAQKLGVPLLPLPPTVYHGVAEDPEEGLVLER-------NWRDRGAMIIYTSGT 228

Query: 232 TGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGSTVE 291
           TG+PKG + TH +I + V  L   W +T  D  LH LPLHHVHG+ N LL P++ G+T  
Sbjct: 229 TGRPKGVLSTHDNIRAVVTGLVHKWAWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCV 288

Query: 292 FMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEA--MEPELQ-VAS 351
            +P+FS   +W+++        S     I VF  VPTIYS+L+  Y+    +P +Q    
Sbjct: 289 MLPEFSAQLVWEKFL-------SSEAPQINVFMAVPTIYSKLMDYYDKHFTQPHVQDFVR 348

Query: 352 ASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPLNGNRKIGTVG 411
           A    ++RLM+ GS+ALP P++++WK +TGH LLERYGMTE  MA+SNPL   R  G+VG
Sbjct: 349 AVCEEKIRLMVSGSAALPLPVLEKWKGITGHTLLERYGMTEIGMALSNPLTAARLPGSVG 408

Query: 412 KPFPGVEAKIVADDGSTDDS--------------------NLEGELCIRSPSMFKEYWKL 471
            P PGVE +IV+++   D S                      EGEL +R PS+F+EYW  
Sbjct: 409 TPLPGVEVRIVSENPQKDSSPYLIHAEGSEENTKVTPGFEEKEGELLVRGPSVFREYWDK 468

Query: 472 PEVTKASFIDGGFFKTGDAVRLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPS 531
           PE TKA+F   G+FKTGD V + +DG Y I GR + DI+K GG+K+SALE+E ++L HPS
Sbjct: 469 PEETKAAFTSDGWFKTGDTV-VFKDGCYWIRGRTSVDIIKSGGYKVSALEVERLLLAHPS 528

Query: 532 VIECCVLGLPDKDYGERVCAIIVLNSKTTTTPGESKPTMSLDELRTWAKDKLAPYKLPTM 591
           + +  V+G+PD  +G+RV A++ L         +   ++S  EL+ WA+  LAPY +P+ 
Sbjct: 529 ITDVAVIGVPDMTWGQRVTAVVTL---------QEGHSLSHRELKEWARGVLAPYAVPSE 573

Query: 592 LLLKDSLPRNAMGKVNKKELKNKL 593
           LLL + +PRN MGKVNK++L  +L
Sbjct: 589 LLLVEEIPRNQMGKVNKRDLVRQL 573

BLAST of Sed0000326 vs. ExPASy Swiss-Prot
Match: Q3URE1 (Malonate--CoA ligase ACSF3, mitochondrial OS=Mus musculus OX=10090 GN=Acsf3 PE=1 SV=2)

HSP 1 Score: 359.8 bits (922), Expect = 6.1e-98
Identity = 201/532 (37.78%), Postives = 306/532 (57.52%), Query Frame = 0

Query: 84  HSYIQLIKSALKISDLLTRSDLRTGHGHVGGAKIGIVAKPSAEFLAGVLGTWFSGGVAVP 143
           H+Y +L   +L ++  + R       G +   ++  +      ++     +W SGGVAVP
Sbjct: 66  HTYRELYDRSLCLAQEICRLQ-GCKVGDLQEERVSFLCSNDVSYVVAQWASWMSGGVAVP 125

Query: 144 LALSYPEAELLHVLNDSEVSMILSTEDHAESMQSVAAKT-LAKFSLIPPVPHDQSTSIEE 203
           L   +PEA+L + + DS  S+++  +++ E +  +A +  +    L P V H  +    E
Sbjct: 126 LYWKHPEAQLEYFIQDSRSSLVVVGQEYLERLSPLAQRLGVPLLPLTPAVYHGATEKPTE 185

Query: 204 DAADAERVFQGTVENLSDDPALIIYTSGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSAD 263
              +             D  A+I YTSGTTG+PKGA+ TH+++ + V  L  +W +T  D
Sbjct: 186 QPVEE--------SGWRDRGAMIFYTSGTTGRPKGALSTHRNLAAVVTGLVHSWAWTKND 245

Query: 264 QFLHCLPLHHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITV 323
             LH LPLHHVHG+ N LL P++ G+T   +P+FS   +W+++        S     ITV
Sbjct: 246 VILHVLPLHHVHGVVNKLLCPLWVGATCVMLPEFSAQQVWEKFL-------SSEAPQITV 305

Query: 324 FTGVPTIYSRLLQGYEA--MEPELQ-VASASAARQLRLMMCGSSALPPPIMKQWKVVTGH 383
           F  VPT+YS+LL  Y+    +P +Q    A    ++RLM+ GS+ALP P++++W+  TGH
Sbjct: 306 FMAVPTVYSKLLDYYDKHFTQPHVQDFVRAVCKERIRLMVSGSAALPVPLLEKWRSATGH 365

Query: 384 QLLERYGMTEFVMAISNPLNGNRKIGTVGKPFPGVEAKIVAD------------DGSTDD 443
            LLERYGMTE  MA+SNPL   R  G+VG P PGVE +I+++            +G+   
Sbjct: 366 TLLERYGMTEIGMALSNPLTEARVPGSVGTPLPGVEVRIISENPQKGSPYIIHAEGNERG 425

Query: 444 SNL-------EGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAVRLDEDGYYIILG 503
           + +       EGEL +R PS+F+EYW  PE TK++F   G+F+TGD   + +D  Y I G
Sbjct: 426 TKVTPGFEEKEGELLVRGPSVFREYWDKPEETKSAFTSDGWFRTGDTA-VFKDARYWIRG 485

Query: 504 RMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLNSKTTTTP 563
           R + DI+K GG+K+SALEIE  +L HPS+ +  V+G+PD  +G+RV A++ L        
Sbjct: 486 RTSVDIIKTGGYKVSALEIERHLLAHPSITDVAVIGVPDMTWGQRVTAVVAL-------- 545

Query: 564 GESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKELKNKL 593
            +   ++S  +L+ WA+  LAPY +P+ LLL + +PRN MGKVNKKEL  +L
Sbjct: 546 -QEGHSLSHGDLKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKKELLKQL 571

BLAST of Sed0000326 vs. ExPASy Swiss-Prot
Match: Q4G176 (Malonate--CoA ligase ACSF3, mitochondrial OS=Homo sapiens OX=9606 GN=ACSF3 PE=1 SV=3)

HSP 1 Score: 358.6 bits (919), Expect = 1.4e-97
Identity = 206/538 (38.29%), Postives = 313/538 (58.18%), Query Frame = 0

Query: 75  VAIRANQKSHSYIQLIKSALKISDLLTRSDLRTGHGHVGGAKIGIVAKPSAEFLAGVLGT 134
           +A+      H+Y +L   +L++S  + R     G G +   ++  +    A ++     +
Sbjct: 57  IALVDQHGRHTYRELYSRSLRLSQEICRLCGCVG-GDLREERVSFLCANDASYVVAQWAS 116

Query: 135 WFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHAESMQSVAAKTLAKFSLIPPVPH 194
           W SGGVAVPL   +P A+L +V+ DS+ S++L+++++ E +  V  K      L+P  P 
Sbjct: 117 WMSGGVAVPLYRKHPAAQLEYVICDSQSSVVLASQEYLELLSPVVRK--LGVPLLPLTPA 176

Query: 195 DQSTSIEEDAADAERVFQGTVENLSDDPALIIYTSGTTGKPKGAIHTHKSITSQVQTLSK 254
             + ++EE A           +   +  A+IIYTSGTTG+PKG + TH++I + V  L  
Sbjct: 177 IYTGAVEEPAE-----VPVPEQGWRNKGAMIIYTSGTTGRPKGVLSTHQNIRAVVTGLVH 236

Query: 255 AWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWQRWRESYPSNDS 314
            W +T  D  LH LPLHHVHG+ N LL P++ G+T   MP+FS   +W+++        S
Sbjct: 237 KWAWTKDDVILHVLPLHHVHGVVNALLCPLWVGATCVMMPEFSPQQVWEKFL-------S 296

Query: 315 KSDDAITVFTGVPTIYSRLLQGYEA--MEPELQ-VASASAARQLRLMMCGSSALPPPIMK 374
                I VF  VPTIY++L++ Y+    +P  Q    A    ++RLM+ GS+ALP P+++
Sbjct: 297 SETPRINVFMAVPTIYTKLMEYYDRHFTQPHAQDFLRAVCEEKIRLMVSGSAALPLPVLE 356

Query: 375 QWKVVTGHQLLERYGMTEFVMAISNPL-NGNRKIGTVGKPFPGVEAKIVADDGSTDDSNL 434
           +WK +TGH LLERYGMTE  MA+S PL    R  G+VG P PGV+ +IV+++   +  + 
Sbjct: 357 KWKNITGHTLLERYGMTEIGMALSGPLTTAVRLPGSVGTPLPGVQVRIVSENPQREACSY 416

Query: 435 --------------------EGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAVRL 494
                               EGEL +R PS+F+EYW  PE TK++F   G+FKTGD V +
Sbjct: 417 TIHAEGDERGTKVTPGFEEKEGELLVRGPSVFREYWNKPEETKSAFTLDGWFKTGDTV-V 476

Query: 495 DEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAII 554
            +DG Y I GR + DI+K GG+K+SALE+E  +L HPS+ +  V+G+PD  +G+RV A++
Sbjct: 477 FKDGQYWIRGRTSVDIIKTGGYKVSALEVEWHLLAHPSITDVAVIGVPDMTWGQRVTAVV 536

Query: 555 VLNSKTTTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKEL 589
            L        G S   +S  EL+ WA++ LAPY +P+ L+L + +PRN MGK++KK L
Sbjct: 537 TLRE------GHS---LSHRELKEWARNVLAPYAVPSELVLVEEIPRNQMGKIDKKAL 569

BLAST of Sed0000326 vs. ExPASy TrEMBL
Match: A0A1S3BUN8 (malonate--CoA ligase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493370 PE=3 SV=1)

HSP 1 Score: 1037.3 bits (2681), Expect = 2.4e-299
Identity = 527/609 (86.54%), Postives = 556/609 (91.30%), Query Frame = 0

Query: 1   MNSSSFNYPTC----ICHLSFPPSHLIAISSSSACFRLGRTISKLCFPRSRLFSVLSSPT 60
           MNSS FN PT     ICHLS P SHLIAI SSSACF +G++I K CF RSRLFSVLSS T
Sbjct: 1   MNSSPFNNPTSLFTRICHLSLPSSHLIAIRSSSACFCIGKSIPKFCFSRSRLFSVLSSYT 60

Query: 61  FMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRTGHGHVGGAK 120
           FMEVVKSAAKHG+TC ++VAIRANQKSHSYIQLI SALKISDLLT SDLRTGHGH+GGAK
Sbjct: 61  FMEVVKSAAKHGATCRESVAIRANQKSHSYIQLISSALKISDLLTSSDLRTGHGHLGGAK 120

Query: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHAESMQ 180
           IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDH E MQ
Sbjct: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHVEFMQ 180

Query: 181 SVAAKTLAKFSLIPPVP--------HDQSTSIEEDAADAERVFQGTVENLSDDPALIIYT 240
           SVAAKT+A  SLIPPVP        HDQST+IE +AADA+RVFQGTVENLSDDPALIIYT
Sbjct: 181 SVAAKTVAIVSLIPPVPHIYSERNSHDQSTNIEVNAADADRVFQGTVENLSDDPALIIYT 240

Query: 241 SGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS 300
           SGTTGKPKG +HTHKSITSQVQTLS AWEYTSADQFLHCLPLHHVHG+FNGLLAPIYAGS
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQTLSNAWEYTSADQFLHCLPLHHVHGMFNGLLAPIYAGS 300

Query: 301 TVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEAMEPELQVAS 360
           TVEFMPKFSVSGIW+RWRESYPS  SKSDDAITVFTGVPT+Y+RLLQGYEAM+ ELQ+AS
Sbjct: 301 TVEFMPKFSVSGIWKRWRESYPSKSSKSDDAITVFTGVPTMYARLLQGYEAMDSELQLAS 360

Query: 361 ASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPLNGNRKIGTVG 420
           ASAARQLRLMMCGSSALPPPIMKQW+ VTGH+LLERYGMTEFVMAISNPLNG RKIGTVG
Sbjct: 361 ASAARQLRLMMCGSSALPPPIMKQWREVTGHELLERYGMTEFVMAISNPLNGQRKIGTVG 420

Query: 421 KPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAV 480
           KPFPGVEAKIVADDGST  S+LEGELCIRSPSMFKEYWKLP+VTK SFIDGGFFKTGDA 
Sbjct: 421 KPFPGVEAKIVADDGSTTGSSLEGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAA 480

Query: 481 RLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540
           RLDEDGYYIILGR++ADIMKVGG+KLSALEIESVILQHPSV ECCVLGLPDKDYGERVCA
Sbjct: 481 RLDEDGYYIILGRLSADIMKVGGYKLSALEIESVILQHPSVTECCVLGLPDKDYGERVCA 540

Query: 541 IIVLNSKT-TTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKE 597
           I+VL + T TT P  S P+MSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKE
Sbjct: 541 IVVLQANTKTTIPDGSNPSMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKE 600

BLAST of Sed0000326 vs. ExPASy TrEMBL
Match: A0A6J1KFM3 (malonate--CoA ligase OS=Cucurbita maxima OX=3661 GN=LOC111494602 PE=4 SV=1)

HSP 1 Score: 1030.8 bits (2664), Expect = 2.3e-297
Identity = 526/609 (86.37%), Postives = 551/609 (90.48%), Query Frame = 0

Query: 1   MNSSSFNYPTC----ICHLSFPPSHLIAISSSSACFRLGRTISKLCFPRSRLFSVLSSPT 60
           MNS +FNY T     I HLSF PSHLIAI S SAC RL R+I KLCFPRSRLFSV SS T
Sbjct: 1   MNSFTFNYSTYLFSRIYHLSFAPSHLIAIQSCSACIRLHRSIPKLCFPRSRLFSVSSSST 60

Query: 61  FMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRTGHGHVGGAK 120
           FMEVVKSAAKHGSTC +NVAIRAN KSHSY+QLI SALKISDLLT  DLRTGHGH+GGAK
Sbjct: 61  FMEVVKSAAKHGSTCRENVAIRANMKSHSYMQLISSALKISDLLTSCDLRTGHGHLGGAK 120

Query: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHAESMQ 180
           IGI+AKPSAEFLAG+LGTWFSGGVAVPLAL+YPE ELLHVLNDSEVSMILSTEDHAE MQ
Sbjct: 121 IGIIAKPSAEFLAGLLGTWFSGGVAVPLALNYPEPELLHVLNDSEVSMILSTEDHAEIMQ 180

Query: 181 SVAAKTLAKFSLIPPVPH--------DQSTSIEEDAADAERVFQGTVENLSDDPALIIYT 240
           SVAAKT AKFSLIPPVPH        DQST IE    DAERVFQGTVENLSDDPAL+IYT
Sbjct: 181 SVAAKTAAKFSLIPPVPHIYSETNSRDQSTDIE---MDAERVFQGTVENLSDDPALVIYT 240

Query: 241 SGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS 300
           SGTTGKPKG +HTHKSITSQVQTLSKAWEYTSADQ LHCLPLHHVHGLFNG LAPIYAGS
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQLLHCLPLHHVHGLFNGFLAPIYAGS 300

Query: 301 TVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEAMEPELQVAS 360
           TVEFMPKFSVSGIW+RWRESYPSN SK+DDAIT+FTGVPT+Y+RLLQGYEAMEP+LQV S
Sbjct: 301 TVEFMPKFSVSGIWKRWRESYPSNGSKNDDAITIFTGVPTMYARLLQGYEAMEPDLQVDS 360

Query: 361 ASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPLNGNRKIGTVG 420
           ASAARQLRLMMCGSSALP PIMKQWK VTGHQLLERYGMTEFVMAISNPLNG RKIGTVG
Sbjct: 361 ASAARQLRLMMCGSSALPTPIMKQWKAVTGHQLLERYGMTEFVMAISNPLNGERKIGTVG 420

Query: 421 KPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAV 480
           KP PGVEAKIV+DDGS +D+NLEGELCIRSPSMFKEYWKLP+VTK SFIDGGFFKTGDA 
Sbjct: 421 KPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAA 480

Query: 481 RLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540
           RLDEDGYY ILGRMNADIMKVGG+KLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA
Sbjct: 481 RLDEDGYYTILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540

Query: 541 IIVL--NSKTTTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKK 596
           IIVL  NSK  T P +SKP MSLD+LRTWAKDKLAPYK+PT+LLLKDSLPRNAMGKVNKK
Sbjct: 541 IIVLHPNSK-ATPPDQSKPNMSLDDLRTWAKDKLAPYKIPTVLLLKDSLPRNAMGKVNKK 600

BLAST of Sed0000326 vs. ExPASy TrEMBL
Match: A0A6J1HKE3 (malonate--CoA ligase OS=Cucurbita moschata OX=3662 GN=LOC111464352 PE=4 SV=1)

HSP 1 Score: 1030.8 bits (2664), Expect = 2.3e-297
Identity = 523/609 (85.88%), Postives = 551/609 (90.48%), Query Frame = 0

Query: 1   MNSSSFNYPTC----ICHLSFPPSHLIAISSSSACFRLGRTISKLCFPRSRLFSVLSSPT 60
           MNS +FNY T     ICHLSF PSHLIAI S S C RL R+I KLCFPRSRLFSV SS T
Sbjct: 1   MNSFTFNYSTYLFRHICHLSFAPSHLIAIQSCSTCLRLHRSIPKLCFPRSRLFSVSSSST 60

Query: 61  FMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRTGHGHVGGAK 120
           FMEVVKSAA HGSTCH+NVAIRAN KSHSY+QLI SALKISDL T  DLRTGHGH+ GAK
Sbjct: 61  FMEVVKSAANHGSTCHENVAIRANMKSHSYMQLISSALKISDLFTSCDLRTGHGHLDGAK 120

Query: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHAESMQ 180
           IGI+AKPSAEFLAG+LGTWFSGGVAVPLAL+YPE ELLHVLNDSEVSMILSTEDHAE MQ
Sbjct: 121 IGIIAKPSAEFLAGLLGTWFSGGVAVPLALNYPEPELLHVLNDSEVSMILSTEDHAEIMQ 180

Query: 181 SVAAKTLAKFSLIPPVPH--------DQSTSIEEDAADAERVFQGTVENLSDDPALIIYT 240
           SVAAKT AKFSLIPPVPH        DQST IE    DA+RVFQGTVEN SDDPAL+IYT
Sbjct: 181 SVAAKTAAKFSLIPPVPHIYSEINSRDQSTDIE---MDAQRVFQGTVENSSDDPALVIYT 240

Query: 241 SGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS 300
           SGTTGKPKG +HTHKSITSQVQTLSKAWEYTSADQ LHCLPLHHVHGLFNGLLAPIYAGS
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQLLHCLPLHHVHGLFNGLLAPIYAGS 300

Query: 301 TVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEAMEPELQVAS 360
           TVEFMPKFSVSGIW+RWRESYPSN SK+DDAIT+FTGVPT+Y+RLLQGYEAMEP+LQVAS
Sbjct: 301 TVEFMPKFSVSGIWKRWRESYPSNGSKNDDAITIFTGVPTMYARLLQGYEAMEPDLQVAS 360

Query: 361 ASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPLNGNRKIGTVG 420
           A+AARQLRLMMCGSSALPPPIMKQWK VTGHQLLERYGMTEFVMAISNPLNG RKIGTVG
Sbjct: 361 ANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMTEFVMAISNPLNGERKIGTVG 420

Query: 421 KPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAV 480
           KP PGVEAKIV+DDGS +D+NLEGELCIRSPSMFKEYWKLP+VTK SFIDGGFFKTGDA 
Sbjct: 421 KPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAA 480

Query: 481 RLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540
           RLDEDGYY ILGRM+ADIMKVGG+KLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA
Sbjct: 481 RLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540

Query: 541 IIVL--NSKTTTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKK 596
           IIVL  NSK  T P +SKP MSLD+LRTWAKDKLAPYK+PT+LLLKDSLPRNAMGKVNKK
Sbjct: 541 IIVLHPNSK-ATPPDQSKPNMSLDDLRTWAKDKLAPYKIPTVLLLKDSLPRNAMGKVNKK 600

BLAST of Sed0000326 vs. ExPASy TrEMBL
Match: A0A0A0L7U7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G099620 PE=3 SV=1)

HSP 1 Score: 1030.4 bits (2663), Expect = 3.0e-297
Identity = 528/610 (86.56%), Postives = 554/610 (90.82%), Query Frame = 0

Query: 1   MNSSSFNYPTC----ICHLSFPPSHLIAISSSSACFRLGRTISKLCFPRSRLFSVLSSPT 60
           MN S+FN PT     I HLS P S LIAI SSSACF +G++I K CF R RLFSVLSS T
Sbjct: 1   MNYSTFNNPTSLFTRISHLSLPSSRLIAIRSSSACFCIGKSIPKFCFSRFRLFSVLSSYT 60

Query: 61  FMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRTGHGHVGGAK 120
           FMEVVKSAAKHG+TC +NVAIRANQKSHSYIQLI SALKISDLLT SDLRTGHGH+GGAK
Sbjct: 61  FMEVVKSAAKHGATCRENVAIRANQKSHSYIQLISSALKISDLLTSSDLRTGHGHLGGAK 120

Query: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHAESMQ 180
           IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDH E MQ
Sbjct: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHVEFMQ 180

Query: 181 SVAAKTLAKFSLIPPVP--------HDQSTSIEEDAADAERVFQGTVENLSDDPALIIYT 240
           SVAAKT+A  SLIPPVP        HDQST+IE DAADA+RVFQGTVENLSDDPALIIYT
Sbjct: 181 SVAAKTVATVSLIPPVPHIYSERNSHDQSTNIEVDAADADRVFQGTVENLSDDPALIIYT 240

Query: 241 SGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS 300
           SGTTGKPKG +HTHKSITSQVQ LS AWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQMLSNAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS 300

Query: 301 TVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEAMEPELQVAS 360
           TVEFMPKFSVSGIW+RWRESYPS  SKSDDAITVFTGVPT+Y+RLLQGYEAM+ ELQ+AS
Sbjct: 301 TVEFMPKFSVSGIWKRWRESYPSKSSKSDDAITVFTGVPTMYARLLQGYEAMDSELQLAS 360

Query: 361 ASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPLNGNRKIGTVG 420
           ASAARQLRLMMCGSSALPPPIMKQWK VTGH+LLERYGMTEFVMAISNPLNG RKIGTVG
Sbjct: 361 ASAARQLRLMMCGSSALPPPIMKQWKEVTGHELLERYGMTEFVMAISNPLNGQRKIGTVG 420

Query: 421 KPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAV 480
           KPFPGVEAKIVADDGST+DS+LEGELCIRSPSMFKEYWKLPEVTK SFIDGGFFKTGDA 
Sbjct: 421 KPFPGVEAKIVADDGSTNDSSLEGELCIRSPSMFKEYWKLPEVTKESFIDGGFFKTGDAA 480

Query: 481 RLDED-GYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVC 540
           RLDED GYYIILGR++ADIMKVGG+KLSALEIESVILQHPSVIECCVLGLPDKDYGERVC
Sbjct: 481 RLDEDGGYYIILGRLSADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVC 540

Query: 541 AIIVLNSK-TTTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKK 597
           AI+VL +   TT P  S P+MSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKK
Sbjct: 541 AIVVLQANMKTTIPDGSNPSMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKK 600

BLAST of Sed0000326 vs. ExPASy TrEMBL
Match: A0A1S3BSX8 (malonate--CoA ligase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493370 PE=3 SV=1)

HSP 1 Score: 971.8 bits (2511), Expect = 1.3e-279
Identity = 502/609 (82.43%), Postives = 531/609 (87.19%), Query Frame = 0

Query: 1   MNSSSFNYPTC----ICHLSFPPSHLIAISSSSACFRLGRTISKLCFPRSRLFSVLSSPT 60
           MNSS FN PT     ICHLS P SHLIAI SSSACF +G++I K CF RSRLFSVLSS T
Sbjct: 1   MNSSPFNNPTSLFTRICHLSLPSSHLIAIRSSSACFCIGKSIPKFCFSRSRLFSVLSSYT 60

Query: 61  FMEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRTGHGHVGGAK 120
           FMEVVKSAAKHG+TC ++VAIRANQKSHSYIQLI SALKISDLLT SDLRTGHGH+GGAK
Sbjct: 61  FMEVVKSAAKHGATCRESVAIRANQKSHSYIQLISSALKISDLLTSSDLRTGHGHLGGAK 120

Query: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHAESMQ 180
           IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDH E MQ
Sbjct: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHVEFMQ 180

Query: 181 SVAAKTLAKFSLIPPVP--------HDQSTSIEEDAADAERVFQGTVENLSDDPALIIYT 240
           SVAAKT+A  SLIPPVP        HDQST+IE +AADA+RVFQGTVENLSDDPALIIYT
Sbjct: 181 SVAAKTVAIVSLIPPVPHIYSERNSHDQSTNIEVNAADADRVFQGTVENLSDDPALIIYT 240

Query: 241 SGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNGLLAPIYAGS 300
           SGTTGKPKG +HTHKSITSQVQTLS AWEYTSADQFLHCLPLHHVHG+FNGLLAPIYAGS
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQTLSNAWEYTSADQFLHCLPLHHVHGMFNGLLAPIYAGS 300

Query: 301 TVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEAMEPELQVAS 360
           TVEFMPKFSVSGIW+RWRESYPS  SKSDDAITVFTGVPT+Y+RLLQGYEAM+ ELQ+AS
Sbjct: 301 TVEFMPKFSVSGIWKRWRESYPSKSSKSDDAITVFTGVPTMYARLLQGYEAMDSELQLAS 360

Query: 361 ASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPLNGNRKIGTVG 420
           ASAARQLRLMMCGSSALPPPIMKQW+ VTGH+LLERYGMT                    
Sbjct: 361 ASAARQLRLMMCGSSALPPPIMKQWREVTGHELLERYGMT-------------------- 420

Query: 421 KPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAV 480
                 EAKIVADDGST  S+LEGELCIRSPSMFKEYWKLP+VTK SFIDGGFFKTGDA 
Sbjct: 421 ------EAKIVADDGSTTGSSLEGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAA 480

Query: 481 RLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 540
           RLDEDGYYIILGR++ADIMKVGG+KLSALEIESVILQHPSV ECCVLGLPDKDYGERVCA
Sbjct: 481 RLDEDGYYIILGRLSADIMKVGGYKLSALEIESVILQHPSVTECCVLGLPDKDYGERVCA 540

Query: 541 IIVLNSKT-TTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKE 597
           I+VL + T TT P  S P+MSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKE
Sbjct: 541 IVVLQANTKTTIPDGSNPSMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKE 583

BLAST of Sed0000326 vs. TAIR 10
Match: AT3G16170.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 717.2 bits (1850), Expect = 1.1e-206
Identity = 362/554 (65.34%), Postives = 438/554 (79.06%), Query Frame = 0

Query: 58  MEVVKSAAKHGSTCHKNVAIRANQKSHSYIQLIKSALKISDLLTRSDLRT---------G 117
           MEV K+A    S     +AI+A+ KS+SY QL  SAL+IS L  + D            G
Sbjct: 1   MEVFKAAFSEASNSCDRIAIKADGKSYSYGQLTSSALRISKLFLKDDTTNGGQETKKYEG 60

Query: 118 HGHVGGAKIGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILST 177
            G + GA+IGIVAKPSAEF+AGVLGTWFSGGVAVPLALSYPEAELLHV+NDS++S++LST
Sbjct: 61  FGSLKGARIGIVAKPSAEFVAGVLGTWFSGGVAVPLALSYPEAELLHVMNDSDISLLLST 120

Query: 178 EDHAESMQSVAAKTLAKFSLIPPVPHDQSTSI-----EEDAADAERVFQGTVENLSDDPA 237
           EDH+E+M+++AAK+ A+F LIPPV +  S ++     ++D+ +AE  F        DDPA
Sbjct: 121 EDHSETMKTIAAKSGARFHLIPPVVNSTSETVACNQFQDDSFEAEGKF-------LDDPA 180

Query: 238 LIIYTSGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNGLLAP 297
           LI+YTSGTTGKPKG +HTH SI SQV+ L++AWEYTSAD FLHCLPLHHVHGLFN L AP
Sbjct: 181 LIVYTSGTTGKPKGVVHTHNSINSQVRMLTEAWEYTSADHFLHCLPLHHVHGLFNALFAP 240

Query: 298 IYAGSTVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEAMEPE 357
           +YA S VEF+PKFSVSGIW+RWRESYP ND K++D+ITVFTGVPT+Y+RL+QGYEAM+ E
Sbjct: 241 LYARSLVEFLPKFSVSGIWRRWRESYPVNDEKTNDSITVFTGVPTMYTRLIQGYEAMDKE 300

Query: 358 LQVASASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMTEFVMAISNPLNGNRK 417
           +Q +SA AAR+LRLMM GSSALP P+M QW+ +TGH+LLERYGMTEFVMA+SNPL G R 
Sbjct: 301 MQDSSAFAARKLRLMMSGSSALPRPVMHQWESITGHRLLERYGMTEFVMAMSNPLRGARN 360

Query: 418 IGTVGKPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVTKASFIDGGFFK 477
            GTVGKP PGVEAKI  D+   +D+N  GE+C++SPS+FKEYW LPEVTK SF + G+FK
Sbjct: 361 AGTVGKPLPGVEAKIKEDE---NDANGVGEICVKSPSLFKEYWNLPEVTKESFTEDGYFK 420

Query: 478 TGDAVRLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIECCVLGLPDKDYG 537
           TGDA R+DEDGYY+ILGR +ADIMKVGG+KLSALEIES +L+HP+V ECCVLGL D DYG
Sbjct: 421 TGDAGRVDEDGYYVILGRNSADIMKVGGYKLSALEIESTLLEHPTVAECCVLGLTDNDYG 480

Query: 538 ERVCAIIVLNSKT-TTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGK 597
           E V AII+  S        ESKP ++L+EL  WAKDKLAPYKLPT LL+ +SLPRNAMGK
Sbjct: 481 EAVTAIIIAESAAKKRREDESKPVITLEELCGWAKDKLAPYKLPTRLLIWESLPRNAMGK 540

BLAST of Sed0000326 vs. TAIR 10
Match: AT3G48990.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 230.3 bits (586), Expect = 4.0e-60
Identity = 161/510 (31.57%), Postives = 261/510 (51.18%), Query Frame = 0

Query: 95  KISDLLTR--SDLRTGHGHVGGAKIGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAE 154
           ++ DL+ R  S L +  G   G  + +    + EF+   L    +   A PL  +Y   E
Sbjct: 35  RLHDLIERAASRLVSDAGIKPGDVVALTFPNTVEFVIMFLAVIRARATAAPLNAAYTAEE 94

Query: 155 LLHVLNDSEVSMILSTEDHAESMQSVAAKTLAKFSLIPPVPHDQSTSIEEDAADAERVFQ 214
               L+DS+  ++L++++     Q  A+K   K S +     D  + +    AD++ V  
Sbjct: 95  FEFYLSDSDSKLLLTSKEGNAPAQEAASK--LKISHVTATLLDAGSDLVLSVADSDSVVD 154

Query: 215 GTVE--NLSDDPALIIYTSGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPL 274
              E  N  DD AL ++TSGTT +PKG   T  ++ S V+ +   ++ T +D  +  LPL
Sbjct: 155 SATELVNHPDDGALFLHTSGTTSRPKGVPLTQLNLASSVKNIKAVYKLTESDSTVIVLPL 214

Query: 275 HHVHGLFNGLLAPIYAGS--TVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPT 334
            HVHGL  GLL+ + AG+  T+    +FS +  W          D K  +A T +T VPT
Sbjct: 215 FHVHGLLAGLLSSLGAGAAVTLPAAGRFSATTFWP---------DMKKYNA-TWYTAVPT 274

Query: 335 IYSRLLQGYEAMEPELQVASASAARQLRLMMCGSSALPPPIMKQWKVVTGHQLLERYGMT 394
           I+  +L  + A  PE +        +LR +   S++L P I+ + +   G  +LE Y MT
Sbjct: 275 IHQIILDRH-ASHPETEYP------KLRFIRSCSASLAPVILSRLEEAFGAPVLEAYAMT 334

Query: 395 EFV-MAISNPL--NGNRKIGTVGKPFPGVEAKIVADDGSTDDSNLEGELCIRSPSMFKEY 454
           E   +  SNPL   G  K G+VGKP  G E  I+ + G   + N +GE+CIR P++ K Y
Sbjct: 335 EATHLMSSNPLPEEGPHKPGSVGKPV-GQEMAILNEKGEIQEPNNKGEVCIRGPNVTKGY 394

Query: 455 WKLPEVTKASFIDGGFFKTGDAVRLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQ 514
              PE  KA F + G+F TGD    D DGY  ++GR+  +++  GG K+S +E+++V+L 
Sbjct: 395 KNNPEANKAGF-EFGWFHTGDIGYFDTDGYLHLVGRIK-ELINRGGEKISPIEVDAVLLT 454

Query: 515 HPSVIECCVLGLPDKDYGERVCAIIVLNSKTTTTPGESKPTMSLDELRTWAKDKLAPYKL 574
           HP V +    G+PD+ YGE +   ++    TT T          ++++ + K  LA +K+
Sbjct: 455 HPDVSQGVAFGVPDEKYGEEINCAVIPREGTTVTE---------EDIKAFCKKNLAAFKV 513

Query: 575 PTMLLLKDSLPRNAMGKVNKKELKNKLAEE 596
           P  + + D+LP+ A GK+ ++ +     E+
Sbjct: 515 PKRVFITDNLPKTASGKIQRRIVAQHFLEK 513

BLAST of Sed0000326 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 156.4 bits (394), Expect = 7.3e-38
Identity = 106/385 (27.53%), Postives = 197/385 (51.17%), Query Frame = 0

Query: 220 DDPALIIYTSGTTGKPKGAIHTHKSITSQVQTL----SKAWEYT-SADQFLHCLPLHHVH 279
           DD A I+Y+SGTTG  KG + TH+++ + ++      +  +EY  S++ +L  LPL H++
Sbjct: 204 DDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPLCHIY 263

Query: 280 GLFNGLLAPIYAGSTVEFMPKFSVS---GIWQRWRESYPSNDSKSDDAITVFTGVPTIYS 339
           GL   ++  +  GST+  M +F  S    + +R++             IT F  VP +  
Sbjct: 264 GLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFK-------------ITHFPVVPPMLM 323

Query: 340 RLLQGYEAMEPELQVASASAARQLRLMMCGSSALPPPIMKQWKVVTGH-QLLERYGMTEF 399
                  A+  + +       + L+ +  G++ L    ++ +     H  L++ YGMTE 
Sbjct: 324 -------ALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTES 383

Query: 400 VMAISNPLNGNR--KIGTVGKPFPGVEAKIVA-DDGSTDDSNLEGELCIRSPSMFKEYWK 459
               +   N  +  +  +VG   P ++AK+V    GS       GEL I+ P + K Y  
Sbjct: 384 TAVGTRGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLN 443

Query: 460 LPEVTKASFIDGGFFKTGDAVRLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHP 519
            P+ T+ S ++  + +TGD    DEDGY  I+ R+  +I+K  GF+++  ++E+V++ HP
Sbjct: 444 NPKATQMSIVEDSWLRTGDIAYFDEDGYLFIVDRIK-EIIKYKGFQIAPADLEAVLVSHP 503

Query: 520 SVIECCVLGLPDKDYGERVCAIIVLNSKTTTTPGESKPTMSLDELRTWAKDKLAPYKLPT 579
            +I+  V   P+++ GE   A +V   +T         T+S +++ ++   ++APY+   
Sbjct: 504 LIIDAAVTAAPNEECGEIPVAFVVRRQET---------TLSEEDVISYVASQVAPYRKVR 558

Query: 580 MLLLKDSLPRNAMGKVNKKELKNKL 593
            +++ +S+P++  GK+ +KELK  L
Sbjct: 564 KVVMVNSIPKSPTGKILRKELKRIL 558

BLAST of Sed0000326 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 148.3 bits (373), Expect = 2.0e-35
Identity = 118/519 (22.74%), Postives = 237/519 (45.66%), Query Frame = 0

Query: 85  SYIQLIKSALKISDLLTRSDLRTGHGHVGGAKIGIVAKPSAEFLAGVLGTWFSGGVAVPL 144
           ++ +L ++   ++D L+   +R GH       + +++  S  F    L     G +    
Sbjct: 59  TFTELWRAVESVADCLSEIGIRKGH------VVLLLSPNSILFPVVCLSVMSLGAIITTT 118

Query: 145 ALSYPEAELLHVLNDSEVSMILSTEDHAESMQSVAAKTLAKFSLIPPVPHDQSTSIEEDA 204
                  E+   + DS   +  +T      + + A K       +P V  D+     +  
Sbjct: 119 NPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKK-------LPIVLMDEER--VDSV 178

Query: 205 ADAERVFQGTVENLS----------DDPALIIYTSGTTGKPKGAIHTHKSITSQVQTLSK 264
            D  R+ +   +  S          DD A ++Y+SGTTG  KG I +H+++ + VQT+  
Sbjct: 179 GDVRRLVEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVN 238

Query: 265 AWEYTSADQ-FLHCLPLHHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWQRWRESYPSND 324
            +     +Q F+  +P+ H++GL       +  GST+  + KF          E +    
Sbjct: 239 RFGSDDGEQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKF----------EMHEMMS 298

Query: 325 SKSDDAITVFTGVPTIYSRLLQGYEAMEPELQVASASAARQLRLMMCGSSALPPPIMKQW 384
           +      T    VP I   ++ G + ++ +  ++S      +  ++CG + L   + + +
Sbjct: 299 AIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSS------MHTVLCGGAPLSKEVTEGF 358

Query: 385 -KVVTGHQLLERYGMTEF--VMAISNPLNGNRKIGTVGKPFPGVEAKIVAD-DGSTDDSN 444
            +     ++L+ YG+TE   + A ++ +  +R+ GT GK    +E +IV    G      
Sbjct: 359 AEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPK 418

Query: 445 LEGELCIRSPSMFKEYWKLPEVTKASFIDGGFFKTGDAVRLDEDGYYIILGRMNADIMKV 504
             GEL ++ PS+ K Y+   E T ++    G+ +TGD   +DEDG+  ++ R+  +++K 
Sbjct: 419 QTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLK-ELIKY 478

Query: 505 GGFKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLNSKTTTTPGESKPTMSL 564
            G++++  E+E+++L HP + +  V+  PDK+ G+   A +V  + +         ++S 
Sbjct: 479 KGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGS---------SLSE 536

Query: 565 DELRTWAKDKLAPYKLPTMLLLKDSLPRNAMGKVNKKEL 589
             +  +   ++APYK    +    S+P+N  GK+ +K+L
Sbjct: 539 KTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDL 536

BLAST of Sed0000326 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 144.1 bits (362), Expect = 3.7e-34
Identity = 97/376 (25.80%), Postives = 185/376 (49.20%), Query Frame = 0

Query: 220 DDPALIIYTSGTTGKPKGAIHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHVHGLFNG 279
           DD A+++Y+SGTTG  KG I +H+++T+ V            D F+  +P+ H +GL   
Sbjct: 197 DDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFISD-NLKRDDIFICTVPMFHTYGLLTF 256

Query: 280 LLAPIYAGSTVEFMPKFSVSGIWQRWRESYPSNDSKSDDAITVFTGVPTIYSRLLQGYEA 339
            +  +  GSTV  + +F +          +   D+      T     P +   ++   + 
Sbjct: 257 AMGTVALGSTVVILRRFQL----------HDMMDAVEKHRATALALAPPVLVAMINDADL 316

Query: 340 MEPELQVASASAARQLRLMMCGSSALPPPI----MKQWKVVTGHQLLERYGMTEF--VMA 399
           ++ +  ++S      L+ + CG + L   +    ++++  V    +L+ Y +TE     A
Sbjct: 317 IKAKYDLSS------LKTVRCGGAPLSKEVTEGFLEKYPTV---DILQGYALTESNGGGA 376

Query: 400 ISNPLNGNRKIGTVGKPFPGVEAKIV-ADDGSTDDSNLEGELCIRSPSMFKEYWKLPEVT 459
            +N    +R+ GT G     VEA+IV  + G     N  GEL ++ PS+ K Y+K  E T
Sbjct: 377 FTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEAT 436

Query: 460 KASFIDGGFFKTGDAVRLDEDGYYIILGRMNADIMKVGGFKLSALEIESVILQHPSVIEC 519
             +    G+ KTGD   +DEDG+  ++ R+  +++K  G+++   E+E++++ HP +++ 
Sbjct: 437 NETINLEGWLKTGDLCYIDEDGFLFVVDRLK-ELIKYKGYQVPPAELEALLITHPDILDA 496

Query: 520 CVLGLPDKDYGERVCAIIVLNSKTTTTPGESKPTMSLDELRTWAKDKLAPYKLPTMLLLK 579
            V+  PDK+ G+   A +V   ++          +S  ++  +   ++APYK    +   
Sbjct: 497 AVIPFPDKEAGQYPMAYVVRKHES---------NLSEKQVIDFISKQVAPYKKIRKVSFI 542

Query: 580 DSLPRNAMGKVNKKEL 589
           +S+P+ A GK  +K+L
Sbjct: 557 NSIPKTASGKTLRKDL 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038904615.11.7e-30287.68malonate--CoA ligase isoform X1 [Benincasa hispida][more]
XP_008452304.15.0e-29986.54PREDICTED: malonate--CoA ligase isoform X1 [Cucumis melo][more]
XP_023515293.11.9e-29886.54malonate--CoA ligase [Cucurbita pepo subsp. pepo][more]
XP_022964295.14.7e-29785.88malonate--CoA ligase [Cucurbita moschata][more]
XP_023000346.14.7e-29786.37malonate--CoA ligase [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
M4IS903.0e-22267.93Probable CoA ligase CCL8 OS=Humulus lupulus OX=3486 GN=CCL8 PE=2 SV=1[more]
Q8H1513.6e-20761.71Malonate--CoA ligase OS=Arabidopsis thaliana OX=3702 GN=AAE13 PE=1 SV=2[more]
Q58DN71.3e-10338.30Malonate--CoA ligase ACSF3, mitochondrial OS=Bos taurus OX=9913 GN=ACSF3 PE=2 SV... [more]
Q3URE16.1e-9837.78Malonate--CoA ligase ACSF3, mitochondrial OS=Mus musculus OX=10090 GN=Acsf3 PE=1... [more]
Q4G1761.4e-9738.29Malonate--CoA ligase ACSF3, mitochondrial OS=Homo sapiens OX=9606 GN=ACSF3 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A1S3BUN82.4e-29986.54malonate--CoA ligase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493370 PE=3 SV=... [more]
A0A6J1KFM32.3e-29786.37malonate--CoA ligase OS=Cucurbita maxima OX=3661 GN=LOC111494602 PE=4 SV=1[more]
A0A6J1HKE32.3e-29785.88malonate--CoA ligase OS=Cucurbita moschata OX=3662 GN=LOC111464352 PE=4 SV=1[more]
A0A0A0L7U73.0e-29786.56Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G099620 PE=3 SV=1[more]
A0A1S3BSX81.3e-27982.43malonate--CoA ligase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493370 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G16170.11.1e-20665.34AMP-dependent synthetase and ligase family protein [more]
AT3G48990.14.0e-6031.57AMP-dependent synthetase and ligase family protein [more]
AT4G19010.17.3e-3827.53AMP-dependent synthetase and ligase family protein [more]
AT1G20510.12.0e-3522.74OPC-8:0 CoA ligase1 [more]
AT1G20500.13.7e-3425.80AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 73..488
e-value: 9.8E-87
score: 291.3
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 498..582
e-value: 3.5E-16
score: 59.9
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 62..481
e-value: 1.7E-105
score: 355.0
NoneNo IPR availableGENE3D3.30.300.30coord: 483..596
e-value: 1.6E-26
score: 94.7
NoneNo IPR availablePANTHERPTHR43201ACYL-COA SYNTHETASEcoord: 39..595
NoneNo IPR availablePANTHERPTHR43201:SF20MALONATE--COA LIGASE-LIKEcoord: 39..595
NoneNo IPR availableCDDcd05941MCScoord: 75..590
e-value: 0.0
score: 657.826
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 65..594
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 225..236

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0000326.1Sed0000326.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0090410 malonate catabolic process
biological_process GO:0009698 phenylpropanoid metabolic process
cellular_component GO:0005829 cytosol
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005634 nucleus
molecular_function GO:0016207 4-coumarate-CoA ligase activity
molecular_function GO:0090409 malonyl-CoA synthetase activity
molecular_function GO:0031956 medium-chain fatty acid-CoA ligase activity