Sed0000099 (gene) Chayote v1

BLAST of Sed0000099 vs. NCBI nr
Match: XP_038874862.1 (ubiquitin-like-specific protease ESD4 isoform X1 [Benincasa hispida])

HSP 1 Score: 804.7 bits (2077), Expect = 4.6e-229
Identity = 414/509 (81.34%), Postives = 457/509 (89.78%), Query Frame = 0

Query: 1   MGARTSNRKRDDECLSVNRSYSSFKSPDFHVSKKLKFSSMSTDPPVVSSNSTAARLSRYP 60
           MGARTSNRKRDDECLS+NRSYSS +SPDFHVSKK KFS+MS+D PVVSSNST ARLSRYP
Sbjct: 1   MGARTSNRKRDDECLSLNRSYSSLRSPDFHVSKKPKFSTMSSDRPVVSSNSTVARLSRYP 60

Query: 61  EETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNRELSEQDKMGNILSYNYQIAKRNAIGA 120
           EET QLRR+VHGPCR+ KFGL   F R  ES+N ELSEQD++GNILS+NYQIAK  AIG+
Sbjct: 61  EETSQLRREVHGPCRLFKFGLSRSFHRFWESKNSELSEQDEVGNILSHNYQIAKSRAIGS 120

Query: 121 LRSFPKDVIELDSDM---------SKDDDEVEVIEDDKRERRSVSEDRRLCEVDTLQELD 180
           LRSFPKDVIELDSD          SK +D+VEV+EDDK       +D+R  EVDT++ELD
Sbjct: 121 LRSFPKDVIELDSDSQTERNASGDSKSEDDVEVVEDDK-------QDQRPREVDTVEELD 180

Query: 181 AKVVDVHQPSSSSVVVDLTSGDSKLENAEKMLGALSLRQDLSSVSAYKKLLQSVEKRTSK 240
            KV++VHQPSSS V+VDLT+ +SK+ENAEKMLGALSL +DLSSVSAYKKLLQSVEKRTS+
Sbjct: 181 EKVMNVHQPSSSLVIVDLTNDNSKVENAEKMLGALSLDRDLSSVSAYKKLLQSVEKRTSR 240

Query: 241 LKSLDFEIELNEKRRSILQSLTPKKKLVDEIPKEIFLPLTKEEEVEVQRAFSTNRRKILV 300
           LKSLDFEIELNEKRRSILQSLTPKKK +D IP+E+F PLTKEEE EV++A S+NRR++LV
Sbjct: 241 LKSLDFEIELNEKRRSILQSLTPKKKPLDVIPRELFTPLTKEEEAEVEQALSSNRRRVLV 300

Query: 301 THDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL 360
            H+NSNIE+TG+  QCLRP AWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL
Sbjct: 301 AHENSNIEITGETFQCLRPAAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL 360

Query: 361 INGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAVINKKEKKFQYLDSLK 420
            NGR+GYDY+SVRRWT+QRKLKYELIDC+KIFVPIHREIHWCLAVINKKEKKFQYLDSLK
Sbjct: 361 -NGRNGYDYRSVRRWTSQRKLKYELIDCDKIFVPIHREIHWCLAVINKKEKKFQYLDSLK 420

Query: 421 GMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS 480
           GMDS VL+TLARY+VDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS
Sbjct: 421 GMDSHVLKTLARYFVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS 480

Query: 481 RGLTLCFKQEHMPYFRLRTAKEILKLIAN 501
           RGL LCFKQEHMPYFRLRTAKEILKL AN
Sbjct: 481 RGLNLCFKQEHMPYFRLRTAKEILKLRAN 501

BLAST of Sed0000099 vs. NCBI nr
Match: XP_004148212.1 (ubiquitin-like-specific protease ESD4 [Cucumis sativus] >KGN47369.1 hypothetical protein Csa_022913 [Cucumis sativus])

HSP 1 Score: 803.9 bits (2075), Expect = 7.8e-229
Identity = 414/509 (81.34%), Postives = 455/509 (89.39%), Query Frame = 0

Query: 1   MGARTSNRKRDDECLSVNRSYSSFKSPDFHVSKKLKFSSMSTDPPVVSSNSTAARLSRYP 60
           MGARTSNRKRDDECLSVNRSYSS +SPDFHVSKK KFS+MSTD PVVSSNST ARLSRYP
Sbjct: 1   MGARTSNRKRDDECLSVNRSYSSLRSPDFHVSKKPKFSTMSTDRPVVSSNSTVARLSRYP 60

Query: 61  EETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNRELSEQDKMGNILSYNYQIAKRNAIGA 120
           EET  LRR+VHGPCR+ KFGL     R  ES+N +LSEQD++GN+LSYNYQ+AK  AIG+
Sbjct: 61  EETSLLRREVHGPCRLFKFGLSRSINRFWESKNSDLSEQDEVGNVLSYNYQVAKSRAIGS 120

Query: 121 LRSFPKDVIELDSDM---------SKDDDEVEVIEDDKRERRSVSEDRRLCEVDTLQELD 180
           LRSFP+DVIELDSD          SK++D+VEVIED+ +E RS        EV T++ELD
Sbjct: 121 LRSFPRDVIELDSDSQTEKDVSGDSKNEDDVEVIEDENQEHRS-------HEVVTMEELD 180

Query: 181 AKVVDVHQPSSSSVVVDLTSGDSKLENAEKMLGALSLRQDLSSVSAYKKLLQSVEKRTSK 240
            KV+DVHQPSSS  VVDLT+ DSK+ENAEKMLGALSL  D+SSV AYKKLLQSVEKRTS+
Sbjct: 181 TKVMDVHQPSSSLEVVDLTNDDSKVENAEKMLGALSLNPDMSSVLAYKKLLQSVEKRTSR 240

Query: 241 LKSLDFEIELNEKRRSILQSLTPKKKLVDEIPKEIFLPLTKEEEVEVQRAFSTNRRKILV 300
           LKSLDFEIELNEKRRS+LQSLTPKKK VDEIP+E+F PLTKEEE EV+RAFS+NRR+ILV
Sbjct: 241 LKSLDFEIELNEKRRSVLQSLTPKKKPVDEIPQELFTPLTKEEEAEVERAFSSNRRRILV 300

Query: 301 THDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL 360
            H+NSNIE+TG+ LQCLRP AWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL
Sbjct: 301 AHENSNIEITGETLQCLRPAAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL 360

Query: 361 INGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAVINKKEKKFQYLDSLK 420
            NGR+GYDY+SV+RWT+QRKLKYELIDC+KIFVPIHREIHWCLAVINKKEKKFQYLDSLK
Sbjct: 361 -NGRNGYDYRSVKRWTSQRKLKYELIDCDKIFVPIHREIHWCLAVINKKEKKFQYLDSLK 420

Query: 421 GMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS 480
           GMDSRVL+TLARY+VDEVKDKSGK+IDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS
Sbjct: 421 GMDSRVLKTLARYFVDEVKDKSGKEIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS 480

Query: 481 RGLTLCFKQEHMPYFRLRTAKEILKLIAN 501
           RGL LCFKQEHMPYFRLRTAKEILKL AN
Sbjct: 481 RGLNLCFKQEHMPYFRLRTAKEILKLRAN 501

BLAST of Sed0000099 vs. NCBI nr
Match: XP_008458450.1 (PREDICTED: ubiquitin-like-specific protease ESD4 [Cucumis melo])

HSP 1 Score: 790.8 bits (2041), Expect = 6.9e-225
Identity = 407/509 (79.96%), Postives = 448/509 (88.02%), Query Frame = 0

Query: 1   MGARTSNRKRDDECLSVNRSYSSFKSPDFHVSKKLKFSSMSTDPPVVSSNSTAARLSRYP 60
           MGARTSNRKRDDEC S+NR YSS +SPDFHV KK KFS+MS D PVVSSNST ARLSRYP
Sbjct: 1   MGARTSNRKRDDECSSINRCYSSLRSPDFHVPKKRKFSTMSADRPVVSSNSTVARLSRYP 60

Query: 61  EETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNRELSEQDKMGNILSYNYQIAKRNAIGA 120
           EET QLRR+VHGPCR+ KFGL     R  ES+N +LSEQD++GN+LSYNYQ+AK  AIG+
Sbjct: 61  EETSQLRREVHGPCRLFKFGLSRSIHRFWESKNSDLSEQDEVGNVLSYNYQVAKSRAIGS 120

Query: 121 LRSFPKDVIELDSDMS---------KDDDEVEVIEDDKRERRSVSEDRRLCEVDTLQELD 180
           LRSFPKDVIELDSD           K++D VEVIED+ +E RS        EV T++ELD
Sbjct: 121 LRSFPKDVIELDSDSQTERDVSGDLKNEDVVEVIEDENQEHRS-------REVVTMEELD 180

Query: 181 AKVVDVHQPSSSSVVVDLTSGDSKLENAEKMLGALSLRQDLSSVSAYKKLLQSVEKRTSK 240
            KV+DV QPSSS  VVDLT+  SK+ENAEKMLGALSL  D+SSV AYKKLLQSVEKRTS+
Sbjct: 181 TKVMDVRQPSSSLEVVDLTNDGSKVENAEKMLGALSLNPDMSSVLAYKKLLQSVEKRTSR 240

Query: 241 LKSLDFEIELNEKRRSILQSLTPKKKLVDEIPKEIFLPLTKEEEVEVQRAFSTNRRKILV 300
           LKSLDFEIELNEKRRSILQSLTPKKK +DEIP+E+F PLTKEEE EV+RAFS+NRR++LV
Sbjct: 241 LKSLDFEIELNEKRRSILQSLTPKKKPLDEIPQELFTPLTKEEEAEVERAFSSNRRRVLV 300

Query: 301 THDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL 360
            H+NSNIE+TG+ LQCLRP AWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL
Sbjct: 301 AHENSNIEITGETLQCLRPAAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL 360

Query: 361 INGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAVINKKEKKFQYLDSLK 420
            NGR+GYDY+SV+RWT+QRKLKYELIDC+KIFVPIHREIHWCLAVINKKEKKFQYLDSLK
Sbjct: 361 -NGRNGYDYRSVKRWTSQRKLKYELIDCDKIFVPIHREIHWCLAVINKKEKKFQYLDSLK 420

Query: 421 GMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS 480
           GMDSRVL+TLARY+VDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS
Sbjct: 421 GMDSRVLKTLARYFVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS 480

Query: 481 RGLTLCFKQEHMPYFRLRTAKEILKLIAN 501
           RGL LCFKQEHMPYFRLRTAKEIL+L AN
Sbjct: 481 RGLNLCFKQEHMPYFRLRTAKEILRLRAN 501

BLAST of Sed0000099 vs. NCBI nr
Match: XP_023006303.1 (ubiquitin-like-specific protease ESD4 [Cucurbita maxima])

HSP 1 Score: 776.9 bits (2005), Expect = 1.0e-220
Identity = 405/510 (79.41%), Postives = 447/510 (87.65%), Query Frame = 0

Query: 1   MGARTSNRKRDDECLSVNRSYSSFKSPDFHVSKKLKFSSMSTDPPVVSSNSTAARLSRYP 60
           MGART+NRKRDDECLS+NRSYSS KSPDFHVSKK K S++S D PV SSN T ARLSRYP
Sbjct: 1   MGARTNNRKRDDECLSLNRSYSSLKSPDFHVSKKPKVSALSLDRPVSSSNYTVARLSRYP 60

Query: 61  EETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNR-ELSEQDKMGNILSYNYQIAKRNAIG 120
           EETPQLRR+VHGPCR+ KFGL   F+R  ES+N  E SEQ   GN+LSYNYQIAK  AIG
Sbjct: 61  EETPQLRREVHGPCRIRKFGLWKSFYRHWESKNNCESSEQYAAGNMLSYNYQIAKNRAIG 120

Query: 121 ALRSFPKDVIELDSDM---------SKDDDEVEVIEDDKRERRSVSEDRRLCEVDTLQEL 180
           ALRSFPKD I LDSD          SK++D +E IEDDK        D+R CEV+T+QEL
Sbjct: 121 ALRSFPKDFINLDSDSETERGASGDSKNEDNIEAIEDDK-------PDQRPCEVNTMQEL 180

Query: 181 DAKVVDVHQPSSSSVVVDLTSGDSKLENAEKMLGALSLRQDLSSVSAYKKLLQSVEKRTS 240
           DAK+ +VHQP S+SVVVDLT+ DSK+ENAE MLGALSL +DLSSVSAYKKLLQ+VE+RTS
Sbjct: 181 DAKMKNVHQP-STSVVVDLTNADSKVENAEAMLGALSLDRDLSSVSAYKKLLQTVERRTS 240

Query: 241 KLKSLDFEIELNEKRRSILQSLTPKKKLVDEIPKEIFLPLTKEEEVEVQRAFSTNRRKIL 300
           +LKSLDFEIELNEKRRSILQS TPKKK +DEIP+E+F PLTKEEEV+V+RA STNRR++L
Sbjct: 241 RLKSLDFEIELNEKRRSILQSRTPKKKPLDEIPQELFTPLTKEEEVQVERALSTNRRRVL 300

Query: 301 VTHDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKK 360
           VTH+NSNIE+TG+ LQCLRP AWLNDEVINLYLELLKERERREPEKYLKCHFF+TFFYKK
Sbjct: 301 VTHENSNIEITGETLQCLRPAAWLNDEVINLYLELLKERERREPEKYLKCHFFSTFFYKK 360

Query: 361 LINGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAVINKKEKKFQYLDSL 420
           L NGR+GYDY SVR+WT  +KLKYELIDC+KIFVPIHREIHWCLAVINKKEKKFQYLDSL
Sbjct: 361 L-NGRNGYDYGSVRKWTNPKKLKYELIDCDKIFVPIHREIHWCLAVINKKEKKFQYLDSL 420

Query: 421 KGMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFY 480
           KGMDSRVL+TLARY+VDEVK+KSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFY
Sbjct: 421 KGMDSRVLKTLARYFVDEVKNKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFY 480

Query: 481 SRGLTLCFKQEHMPYFRLRTAKEILKLIAN 501
           SRGL LCFKQEHMPYFR RTAKEILKL AN
Sbjct: 481 SRGLNLCFKQEHMPYFRRRTAKEILKLRAN 501

BLAST of Sed0000099 vs. NCBI nr
Match: XP_022930536.1 (ubiquitin-like-specific protease ESD4 isoform X3 [Cucurbita moschata])

HSP 1 Score: 773.5 bits (1996), Expect = 1.1e-219
Identity = 402/509 (78.98%), Postives = 433/509 (85.07%), Query Frame = 0

Query: 1   MGARTSNRKRDDECLSVNRSYSSFKSPDFHVSKKLKFSSMSTDPPVVSSNSTAARLSRYP 60
           MGARTSNRKRDDE  S+ RSYSS KSPDFHVSKKLKFS+MS+D PVVSSNST ARLSRYP
Sbjct: 1   MGARTSNRKRDDEYSSIKRSYSSLKSPDFHVSKKLKFSTMSSDRPVVSSNSTVARLSRYP 60

Query: 61  EETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNRELSEQDKMGNILSYNYQIAKRNAIGA 120
           EETPQLRR+VHGPCR  KFGL   FFR  ES+N E SEQD +GNILSYNYQIAKRNAIGA
Sbjct: 61  EETPQLRREVHGPCRGLKFGLSRSFFRFWESKNSEPSEQDDVGNILSYNYQIAKRNAIGA 120

Query: 121 LRSFPKDVIELDSDM---------SKDDDEVEVIEDDKRERRSVSEDRRLCEVDTLQELD 180
           LRSFPKD  ELDSD          SKDD+EVEVIED                  T+QEL 
Sbjct: 121 LRSFPKDAFELDSDTQTERDASGDSKDDEEVEVIED-----------------VTVQELV 180

Query: 181 AKVVDVHQPSSSSVVVDLTSGDSKLENAEKMLGALSLRQDLSSVSAYKKLLQSVEKRTSK 240
           AK +D HQPSSSSVV+DLT  DSK+ENAEKMLG LSL +++SSVS YKKLLQ VEKRTS+
Sbjct: 181 AKKMDAHQPSSSSVVIDLTDEDSKVENAEKMLGVLSLDREISSVSTYKKLLQGVEKRTSR 240

Query: 241 LKSLDFEIELNEKRRSILQSLTPKKKLVDEIPKEIFLPLTKEEEVEVQRAFSTNRRKILV 300
           LK+LDFEIELNEKRRSILQS TPKK  VDEI +E F+PLT++EE EV+ A S NR K+LV
Sbjct: 241 LKTLDFEIELNEKRRSILQSQTPKKMPVDEIQQEPFVPLTRDEEAEVEAALSANRSKVLV 300

Query: 301 THDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL 360
           TH+NSNIE+T Q LQCLRPGAWLNDEVINLYLELLKERERREPEKYL CHFFNTFFYKKL
Sbjct: 301 THENSNIEITAQALQCLRPGAWLNDEVINLYLELLKERERREPEKYLNCHFFNTFFYKKL 360

Query: 361 INGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAVINKKEKKFQYLDSLK 420
           INGRDGYDYKSVRRWT+QRKLKYEL DC+KIFVPIHREIHWCLAVINKKEKKFQYLDS+K
Sbjct: 361 INGRDGYDYKSVRRWTSQRKLKYELNDCDKIFVPIHREIHWCLAVINKKEKKFQYLDSVK 420

Query: 421 GMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS 480
           GMDSRVL+TLARY+VDEVKDKSGKDIDVSSWA EFV+DLPEQ NG+DCGMFMIKY DFYS
Sbjct: 421 GMDSRVLKTLARYFVDEVKDKSGKDIDVSSWAHEFVKDLPEQFNGYDCGMFMIKYTDFYS 480

Query: 481 RGLTLCFKQEHMPYFRLRTAKEILKLIAN 501
           RGL LCFKQEHMPYFR RTAKEIL L AN
Sbjct: 481 RGLDLCFKQEHMPYFRRRTAKEILNLRAN 492

BLAST of Sed0000099 vs. ExPASy Swiss-Prot
Match: Q94F30 (Ubiquitin-like-specific protease ESD4 OS=Arabidopsis thaliana OX=3702 GN=ESD4 PE=1 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 5.1e-130
Identity = 272/524 (51.91%), Postives = 351/524 (66.98%), Query Frame = 0

Query: 1   MGARTSNRKRDDECLSVNRSYSS---FKSPDFHVSKKLKFS-SMSTDP-PVVSSNSTAAR 60
           MGA   NRKR DE  +     S+     SP F  SKK +FS +MS D     SSN T +R
Sbjct: 1   MGAVAINRKRSDESFNFINQQSTNPLRNSPYFQASKKRRFSFAMSEDSGKPASSNPTISR 60

Query: 61  LSRYPEETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNRELSEQDKMGNILSYNYQIAKR 120
           +SRYP+    LRR++H P R           R  ++++ +  E+D   N     Y  AKR
Sbjct: 61  ISRYPDAKAPLRREIHAPSR--------GILRYGKAKSNDYCEKD--ANFFVRKYDDAKR 120

Query: 121 NAIGALR--SFPKDVIELDSDMSK-----DDDEVEVIE----DDKRERRSVSEDRRLCEV 180
           +A+ ALR  +  KD ++L  ++ K     DD  V+ IE    DD  E++++         
Sbjct: 121 SALEALRFVNKGKDFVDLGDEVEKEEVVSDDSSVQAIEVIDCDDDEEKKNL--------- 180

Query: 181 DTLQELDAKVVDVHQPSSSSVVVDLTSGDS-KLENAEKMLGALSLRQDL----SSVSAYK 240
                         QPS SS V D+  G++ ++E+   ML +LSL +D+    SS+ AY+
Sbjct: 181 --------------QPSFSSGVTDVKKGENFRVEDTSMMLDSLSLDRDVDNDASSLEAYR 240

Query: 241 KLLQSVEKRTSKLKSLDFEIELNEKRRSILQSLTPK--KKLVDEIPKEIFLPLTKEEEVE 300
           KL+QS EKR SKL++L FEI LNEK+ S+L+   PK  +K V E+P+E F+PLT++EE E
Sbjct: 241 KLMQSAEKRNSKLEALGFEIVLNEKKLSLLRQSRPKTVEKRV-EVPREPFIPLTEDEEAE 300

Query: 301 VQRAFS-TNRRKILVTHDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEK 360
           V RAFS  NRRK+L TH+NSNI++TG++LQCL P AWLNDEVIN+YLELLKERE REP+K
Sbjct: 301 VYRAFSGRNRRKVLATHENSNIDITGEVLQCLTPSAWLNDEVINVYLELLKERETREPKK 360

Query: 361 YLKCHFFNTFFYKKLINGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAV 420
           YLKCH+FNTFFYKKL++   GY++K+VRRWTTQRKL Y LIDC+ IFVPIHR +HW LAV
Sbjct: 361 YLKCHYFNTFFYKKLVSD-SGYNFKAVRRWTTQRKLGYALIDCDMIFVPIHRGVHWTLAV 420

Query: 421 INKKEKKFQYLDSLKGMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLPEQENG 480
           IN +E K  YLDSL G+D  +L  LA+Y  DE  +KSGK ID +SW  EFVEDLP+Q+NG
Sbjct: 421 INNRESKLLYLDSLNGVDPMILNALAKYMGDEANEKSGKKIDANSWDMEFVEDLPQQKNG 480

Query: 481 FDCGMFMIKYADFYSRGLTLCFKQEHMPYFRLRTAKEILKLIAN 501
           +DCGMFM+KY DF+SRGL LCF QEHMPYFRLRTAKEIL+L A+
Sbjct: 481 YDCGMFMLKYIDFFSRGLGLCFSQEHMPYFRLRTAKEILRLRAD 489

BLAST of Sed0000099 vs. ExPASy Swiss-Prot
Match: Q8GYL3 (Ubiquitin-like-specific protease 1A OS=Arabidopsis thaliana OX=3702 GN=ULP1A PE=2 SV=2)

HSP 1 Score: 401.4 bits (1030), Expect = 1.5e-110
Identity = 225/468 (48.08%), Postives = 301/468 (64.32%), Query Frame = 0

Query: 48  SSNSTAARLSRYPEETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNRELSEQDKMGNILS 107
           SS   A  + RYPE    LRRQVH P R+   G           R+      + +G  L+
Sbjct: 61  SSRPMAPGIYRYPEVKSSLRRQVHAPVRILNSG---------RDRSTRQGSGNVLGTFLT 120

Query: 108 YNYQIAKRNAIGALRSF--PKDVIELDS-----DMSKDDDEVEVIED-----DKRERRSV 167
            N  + KRNA+ +   +   ++VI++D      +M  DD   E +E+     D+ E ++ 
Sbjct: 121 RNNDMWKRNALDSSLRYRTDREVIDVDDELGDVEMISDDTSREGVENVAMEVDEVEEKAE 180

Query: 168 SEDRRLCEVDTLQELDAKVVDVHQPSSSSVVVDLTSGDSKLENAEKMLGALSLRQDLSSV 227
             +    EV +L+    +V +  + +SSS+VV+                      D++S 
Sbjct: 181 MGNGLFSEVASLKNGSLRVGECSKANSSSLVVNRPV------------------TDVTSF 240

Query: 228 SAYKKLLQSVEKRTSKLKSLDFEIELNEKRRSILQSLT----PKKKLVDEIPKEIFLPLT 287
            AY+K+L+S   RTSKLK   F     E+ R++L+SL+      ++ V+ + +E F+PL+
Sbjct: 241 EAYRKVLESAVNRTSKLKDRGFVDFFKERGRALLRSLSSFWRQDEEPVEVVQREAFVPLS 300

Query: 288 KEEEVEVQRAFSTNRRKILVTHDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERER 347
           +EEE  V+RAFS N   ILVTH NSNI++TG+IL+CL+PG WLNDEVINLY+ LLKERE 
Sbjct: 301 REEETAVRRAFSANDSNILVTHKNSNIDITGKILRCLKPGKWLNDEVINLYMVLLKEREA 360

Query: 348 REPEKYLKCHFFNTFFYKKLINGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIH 407
           REP+K+LKCHFFNTFF+ KL+N   GY+Y +VRRWT+ ++L Y L DC+KIF+PIH  IH
Sbjct: 361 REPKKFLKCHFFNTFFFTKLVNSATGYNYGAVRRWTSMKRLGYHLKDCDKIFIPIHMNIH 420

Query: 408 WCLAVINKKEKKFQYLDSLKGMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLP 467
           W LAVIN K++KFQYLDS KG + ++L  LARY+VDEV+DKS  D+DVS W QEFV+DLP
Sbjct: 421 WTLAVINIKDQKFQYLDSFKGREPKILDALARYFVDEVRDKSEVDLDVSRWRQEFVQDLP 480

Query: 468 EQENGFDCGMFMIKYADFYSRGLTLCFKQEHMPYFRLRTAKEILKLIA 500
            Q NGFDCGMFM+KY DFYSRGL LCF QE MPYFR RTAKEIL+L A
Sbjct: 481 MQRNGFDCGMFMVKYIDFYSRGLDLCFTQEQMPYFRARTAKEILQLKA 501

BLAST of Sed0000099 vs. ExPASy Swiss-Prot
Match: O65278 (Putative ubiquitin-like-specific protease 1B OS=Arabidopsis thaliana OX=3702 GN=ULP1B PE=5 SV=2)

HSP 1 Score: 320.1 bits (819), Expect = 4.5e-86
Identity = 172/297 (57.91%), Postives = 214/297 (72.05%), Query Frame = 0

Query: 213 SSVSAYKKLLQSVEKRTSKLKSLDFEIELNEKR-------RSILQSLTPKKKLVDEIPKE 272
           SSVS  KK  +  + +   +K  D  IE +           + L S T  KKLV     E
Sbjct: 48  SSVSLKKKAARCSQSQ-QVVKHNDDRIEEHSSSNRGFKDGNNFLVSSTETKKLV-AFCGE 107

Query: 273 IFLPLTKEEEVEVQRAFS-TNRRKILVTHDNSNIELTGQILQCLRPGAWLNDEVINLYLE 332
            F PL +EE   V  A S  NR+KILV+H NSNI+++G+ LQCLRP  WLND+V NLYLE
Sbjct: 108 PFQPLNEEEVALVNSALSKRNRKKILVSHKNSNIDISGETLQCLRPNQWLNDDVTNLYLE 167

Query: 333 LLKERERREPEKYLKCHFFNTFFYKKLINGRDGYDYKSVRRWTTQRKLKYELIDCEKIFV 392
           LLKER+ R+P+KY KCHFFNTFFY KL++G  GY+YK+V RWTT+RKL Y+LIDC+ IFV
Sbjct: 168 LLKERQTRDPQKYFKCHFFNTFFYVKLVSG-SGYNYKAVSRWTTKRKLGYDLIDCDIIFV 227

Query: 393 PIHREIHWCLAVINKKEKKFQYLDSL-KGMDSRVLRTLARYYVDEVKDKSGKDIDVSSWA 452
           PIH +IHW L VIN +E+KF YLDSL  G+   +L  +A+Y VDEVK KS K+IDVSSW 
Sbjct: 228 PIHIDIHWTLGVINNRERKFVYLDSLFTGVGHTILNAMAKYLVDEVKQKSQKNIDVSSWG 287

Query: 453 QEFVEDLPEQENGFDCGMFMIKYADFYSRGLTLCFKQEHMPYFRLRTAKEILKLIAN 501
            E+VE+ P+Q+NG+DCGMFM+KY DFYSRGL+L F Q+ MPYFRLRTAKEIL+L A+
Sbjct: 288 MEYVEERPQQQNGYDCGMFMLKYIDFYSRGLSLQFSQKDMPYFRLRTAKEILRLRAD 341

BLAST of Sed0000099 vs. ExPASy Swiss-Prot
Match: O42957 (Ubiquitin-like-specific protease 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ulp1 PE=3 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 2.5e-36
Identity = 82/199 (41.21%), Postives = 114/199 (57.29%), Query Frame = 0

Query: 297 NIELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKLINGRD 356
           NI +T + L  LR   WLNDEVIN Y+ L+ ER + +     + H FNTFFY  L   R 
Sbjct: 376 NIPITLKDLHTLRNRQWLNDEVINFYMNLISERSKID-SSLPRVHGFNTFFYTSL--QRR 435

Query: 357 GYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAVINKKEKKFQYLDSLKGMDSR 416
           G  Y  VRRW   +K +  + D + +F+P+H ++HWC+AVINK +K+F+Y DSL G   +
Sbjct: 436 G--YAGVRRWA--KKARVNIADMDAVFIPVHLDVHWCMAVINKSKKRFEYWDSLAGSPGK 495

Query: 417 VLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYSRGLTL 476
           V   L  YY+ E K      +DVS W     ++ P Q NG DCG+F  K A+  SR + +
Sbjct: 496 VFDLLRDYYIAETKGA----VDVSDWENFMDDNSPRQRNGHDCGVFACKTAECVSRNVPV 555

Query: 477 CFKQEHMPYFRLRTAKEIL 496
            F Q  MP  R++ A  I+
Sbjct: 556 QFSQNDMPELRIKMAASII 563

BLAST of Sed0000099 vs. ExPASy Swiss-Prot
Match: Q9HC62 (Sentrin-specific protease 2 OS=Homo sapiens OX=9606 GN=SENP2 PE=1 SV=3)

HSP 1 Score: 153.3 bits (386), Expect = 7.3e-36
Identity = 88/230 (38.26%), Postives = 129/230 (56.09%), Query Frame = 0

Query: 268 LPLTKEEEVEVQRAFSTNRRKILVTHDNSNIELTGQILQCLRPGAWLNDEVINLYLELLK 327
           L LT++ E E+  A     +  +++     + +T   +Q L+   WLNDEVIN Y+ LL 
Sbjct: 366 LELTEDMEKEISNALGHGPQDEILS-SAFKLRITRGDIQTLKNYHWLNDEVINFYMNLLV 425

Query: 328 ERERREPEKYLKCHFFNTFFYKKLINGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIH 387
           ER ++  + Y   H F+TFFY KL +G     Y++V+RWT        L + E I VPIH
Sbjct: 426 ERNKK--QGYPALHVFSTFFYPKLKSG----GYQAVKRWTK----GVNLFEQEIILVPIH 485

Query: 388 REIHWCLAVINKKEKKFQYLDSLKGMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFV 447
           R++HW L VI+ ++K  +YLDS+     R+   L +Y  DE K K   D+++  W    +
Sbjct: 486 RKVHWSLVVIDLRKKCLKYLDSMGQKGHRICEILLQYLQDESKTKRNSDLNLLEWTHHSM 545

Query: 448 E--DLPEQENGFDCGMFMIKYADFYSRGLTLCFKQEHMPYFRLRTAKEIL 496
           +  ++P+Q NG DCGMF  KYAD+ SR   + F Q  MP FR +   EIL
Sbjct: 546 KPHEIPQQLNGSDCGMFTCKYADYISRDKPITFTQHQMPLFRKKMVWEIL 584

BLAST of Sed0000099 vs. ExPASy TrEMBL
Match: A0A0A0KEW0 (ULP_PROTEASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G306310 PE=3 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 3.8e-229
Identity = 414/509 (81.34%), Postives = 455/509 (89.39%), Query Frame = 0

Query: 1   MGARTSNRKRDDECLSVNRSYSSFKSPDFHVSKKLKFSSMSTDPPVVSSNSTAARLSRYP 60
           MGARTSNRKRDDECLSVNRSYSS +SPDFHVSKK KFS+MSTD PVVSSNST ARLSRYP
Sbjct: 1   MGARTSNRKRDDECLSVNRSYSSLRSPDFHVSKKPKFSTMSTDRPVVSSNSTVARLSRYP 60

Query: 61  EETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNRELSEQDKMGNILSYNYQIAKRNAIGA 120
           EET  LRR+VHGPCR+ KFGL     R  ES+N +LSEQD++GN+LSYNYQ+AK  AIG+
Sbjct: 61  EETSLLRREVHGPCRLFKFGLSRSINRFWESKNSDLSEQDEVGNVLSYNYQVAKSRAIGS 120

Query: 121 LRSFPKDVIELDSDM---------SKDDDEVEVIEDDKRERRSVSEDRRLCEVDTLQELD 180
           LRSFP+DVIELDSD          SK++D+VEVIED+ +E RS        EV T++ELD
Sbjct: 121 LRSFPRDVIELDSDSQTEKDVSGDSKNEDDVEVIEDENQEHRS-------HEVVTMEELD 180

Query: 181 AKVVDVHQPSSSSVVVDLTSGDSKLENAEKMLGALSLRQDLSSVSAYKKLLQSVEKRTSK 240
            KV+DVHQPSSS  VVDLT+ DSK+ENAEKMLGALSL  D+SSV AYKKLLQSVEKRTS+
Sbjct: 181 TKVMDVHQPSSSLEVVDLTNDDSKVENAEKMLGALSLNPDMSSVLAYKKLLQSVEKRTSR 240

Query: 241 LKSLDFEIELNEKRRSILQSLTPKKKLVDEIPKEIFLPLTKEEEVEVQRAFSTNRRKILV 300
           LKSLDFEIELNEKRRS+LQSLTPKKK VDEIP+E+F PLTKEEE EV+RAFS+NRR+ILV
Sbjct: 241 LKSLDFEIELNEKRRSVLQSLTPKKKPVDEIPQELFTPLTKEEEAEVERAFSSNRRRILV 300

Query: 301 THDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL 360
            H+NSNIE+TG+ LQCLRP AWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL
Sbjct: 301 AHENSNIEITGETLQCLRPAAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL 360

Query: 361 INGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAVINKKEKKFQYLDSLK 420
            NGR+GYDY+SV+RWT+QRKLKYELIDC+KIFVPIHREIHWCLAVINKKEKKFQYLDSLK
Sbjct: 361 -NGRNGYDYRSVKRWTSQRKLKYELIDCDKIFVPIHREIHWCLAVINKKEKKFQYLDSLK 420

Query: 421 GMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS 480
           GMDSRVL+TLARY+VDEVKDKSGK+IDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS
Sbjct: 421 GMDSRVLKTLARYFVDEVKDKSGKEIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS 480

Query: 481 RGLTLCFKQEHMPYFRLRTAKEILKLIAN 501
           RGL LCFKQEHMPYFRLRTAKEILKL AN
Sbjct: 481 RGLNLCFKQEHMPYFRLRTAKEILKLRAN 501

BLAST of Sed0000099 vs. ExPASy TrEMBL
Match: A0A1S3C941 (ubiquitin-like-specific protease ESD4 OS=Cucumis melo OX=3656 GN=LOC103497852 PE=3 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 3.3e-225
Identity = 407/509 (79.96%), Postives = 448/509 (88.02%), Query Frame = 0

Query: 1   MGARTSNRKRDDECLSVNRSYSSFKSPDFHVSKKLKFSSMSTDPPVVSSNSTAARLSRYP 60
           MGARTSNRKRDDEC S+NR YSS +SPDFHV KK KFS+MS D PVVSSNST ARLSRYP
Sbjct: 1   MGARTSNRKRDDECSSINRCYSSLRSPDFHVPKKRKFSTMSADRPVVSSNSTVARLSRYP 60

Query: 61  EETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNRELSEQDKMGNILSYNYQIAKRNAIGA 120
           EET QLRR+VHGPCR+ KFGL     R  ES+N +LSEQD++GN+LSYNYQ+AK  AIG+
Sbjct: 61  EETSQLRREVHGPCRLFKFGLSRSIHRFWESKNSDLSEQDEVGNVLSYNYQVAKSRAIGS 120

Query: 121 LRSFPKDVIELDSDMS---------KDDDEVEVIEDDKRERRSVSEDRRLCEVDTLQELD 180
           LRSFPKDVIELDSD           K++D VEVIED+ +E RS        EV T++ELD
Sbjct: 121 LRSFPKDVIELDSDSQTERDVSGDLKNEDVVEVIEDENQEHRS-------REVVTMEELD 180

Query: 181 AKVVDVHQPSSSSVVVDLTSGDSKLENAEKMLGALSLRQDLSSVSAYKKLLQSVEKRTSK 240
            KV+DV QPSSS  VVDLT+  SK+ENAEKMLGALSL  D+SSV AYKKLLQSVEKRTS+
Sbjct: 181 TKVMDVRQPSSSLEVVDLTNDGSKVENAEKMLGALSLNPDMSSVLAYKKLLQSVEKRTSR 240

Query: 241 LKSLDFEIELNEKRRSILQSLTPKKKLVDEIPKEIFLPLTKEEEVEVQRAFSTNRRKILV 300
           LKSLDFEIELNEKRRSILQSLTPKKK +DEIP+E+F PLTKEEE EV+RAFS+NRR++LV
Sbjct: 241 LKSLDFEIELNEKRRSILQSLTPKKKPLDEIPQELFTPLTKEEEAEVERAFSSNRRRVLV 300

Query: 301 THDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL 360
            H+NSNIE+TG+ LQCLRP AWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL
Sbjct: 301 AHENSNIEITGETLQCLRPAAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL 360

Query: 361 INGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAVINKKEKKFQYLDSLK 420
            NGR+GYDY+SV+RWT+QRKLKYELIDC+KIFVPIHREIHWCLAVINKKEKKFQYLDSLK
Sbjct: 361 -NGRNGYDYRSVKRWTSQRKLKYELIDCDKIFVPIHREIHWCLAVINKKEKKFQYLDSLK 420

Query: 421 GMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS 480
           GMDSRVL+TLARY+VDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS
Sbjct: 421 GMDSRVLKTLARYFVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS 480

Query: 481 RGLTLCFKQEHMPYFRLRTAKEILKLIAN 501
           RGL LCFKQEHMPYFRLRTAKEIL+L AN
Sbjct: 481 RGLNLCFKQEHMPYFRLRTAKEILRLRAN 501

BLAST of Sed0000099 vs. ExPASy TrEMBL
Match: A0A6J1L4J0 (ubiquitin-like-specific protease ESD4 OS=Cucurbita maxima OX=3661 GN=LOC111499079 PE=3 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 5.0e-221
Identity = 405/510 (79.41%), Postives = 447/510 (87.65%), Query Frame = 0

Query: 1   MGARTSNRKRDDECLSVNRSYSSFKSPDFHVSKKLKFSSMSTDPPVVSSNSTAARLSRYP 60
           MGART+NRKRDDECLS+NRSYSS KSPDFHVSKK K S++S D PV SSN T ARLSRYP
Sbjct: 1   MGARTNNRKRDDECLSLNRSYSSLKSPDFHVSKKPKVSALSLDRPVSSSNYTVARLSRYP 60

Query: 61  EETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNR-ELSEQDKMGNILSYNYQIAKRNAIG 120
           EETPQLRR+VHGPCR+ KFGL   F+R  ES+N  E SEQ   GN+LSYNYQIAK  AIG
Sbjct: 61  EETPQLRREVHGPCRIRKFGLWKSFYRHWESKNNCESSEQYAAGNMLSYNYQIAKNRAIG 120

Query: 121 ALRSFPKDVIELDSDM---------SKDDDEVEVIEDDKRERRSVSEDRRLCEVDTLQEL 180
           ALRSFPKD I LDSD          SK++D +E IEDDK        D+R CEV+T+QEL
Sbjct: 121 ALRSFPKDFINLDSDSETERGASGDSKNEDNIEAIEDDK-------PDQRPCEVNTMQEL 180

Query: 181 DAKVVDVHQPSSSSVVVDLTSGDSKLENAEKMLGALSLRQDLSSVSAYKKLLQSVEKRTS 240
           DAK+ +VHQP S+SVVVDLT+ DSK+ENAE MLGALSL +DLSSVSAYKKLLQ+VE+RTS
Sbjct: 181 DAKMKNVHQP-STSVVVDLTNADSKVENAEAMLGALSLDRDLSSVSAYKKLLQTVERRTS 240

Query: 241 KLKSLDFEIELNEKRRSILQSLTPKKKLVDEIPKEIFLPLTKEEEVEVQRAFSTNRRKIL 300
           +LKSLDFEIELNEKRRSILQS TPKKK +DEIP+E+F PLTKEEEV+V+RA STNRR++L
Sbjct: 241 RLKSLDFEIELNEKRRSILQSRTPKKKPLDEIPQELFTPLTKEEEVQVERALSTNRRRVL 300

Query: 301 VTHDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKK 360
           VTH+NSNIE+TG+ LQCLRP AWLNDEVINLYLELLKERERREPEKYLKCHFF+TFFYKK
Sbjct: 301 VTHENSNIEITGETLQCLRPAAWLNDEVINLYLELLKERERREPEKYLKCHFFSTFFYKK 360

Query: 361 LINGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAVINKKEKKFQYLDSL 420
           L NGR+GYDY SVR+WT  +KLKYELIDC+KIFVPIHREIHWCLAVINKKEKKFQYLDSL
Sbjct: 361 L-NGRNGYDYGSVRKWTNPKKLKYELIDCDKIFVPIHREIHWCLAVINKKEKKFQYLDSL 420

Query: 421 KGMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFY 480
           KGMDSRVL+TLARY+VDEVK+KSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFY
Sbjct: 421 KGMDSRVLKTLARYFVDEVKNKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFY 480

Query: 481 SRGLTLCFKQEHMPYFRLRTAKEILKLIAN 501
           SRGL LCFKQEHMPYFR RTAKEILKL AN
Sbjct: 481 SRGLNLCFKQEHMPYFRRRTAKEILKLRAN 501

BLAST of Sed0000099 vs. ExPASy TrEMBL
Match: A0A6J1ER64 (ubiquitin-like-specific protease ESD4 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111436959 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 5.5e-220
Identity = 402/509 (78.98%), Postives = 433/509 (85.07%), Query Frame = 0

Query: 1   MGARTSNRKRDDECLSVNRSYSSFKSPDFHVSKKLKFSSMSTDPPVVSSNSTAARLSRYP 60
           MGARTSNRKRDDE  S+ RSYSS KSPDFHVSKKLKFS+MS+D PVVSSNST ARLSRYP
Sbjct: 1   MGARTSNRKRDDEYSSIKRSYSSLKSPDFHVSKKLKFSTMSSDRPVVSSNSTVARLSRYP 60

Query: 61  EETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNRELSEQDKMGNILSYNYQIAKRNAIGA 120
           EETPQLRR+VHGPCR  KFGL   FFR  ES+N E SEQD +GNILSYNYQIAKRNAIGA
Sbjct: 61  EETPQLRREVHGPCRGLKFGLSRSFFRFWESKNSEPSEQDDVGNILSYNYQIAKRNAIGA 120

Query: 121 LRSFPKDVIELDSDM---------SKDDDEVEVIEDDKRERRSVSEDRRLCEVDTLQELD 180
           LRSFPKD  ELDSD          SKDD+EVEVIED                  T+QEL 
Sbjct: 121 LRSFPKDAFELDSDTQTERDASGDSKDDEEVEVIED-----------------VTVQELV 180

Query: 181 AKVVDVHQPSSSSVVVDLTSGDSKLENAEKMLGALSLRQDLSSVSAYKKLLQSVEKRTSK 240
           AK +D HQPSSSSVV+DLT  DSK+ENAEKMLG LSL +++SSVS YKKLLQ VEKRTS+
Sbjct: 181 AKKMDAHQPSSSSVVIDLTDEDSKVENAEKMLGVLSLDREISSVSTYKKLLQGVEKRTSR 240

Query: 241 LKSLDFEIELNEKRRSILQSLTPKKKLVDEIPKEIFLPLTKEEEVEVQRAFSTNRRKILV 300
           LK+LDFEIELNEKRRSILQS TPKK  VDEI +E F+PLT++EE EV+ A S NR K+LV
Sbjct: 241 LKTLDFEIELNEKRRSILQSQTPKKMPVDEIQQEPFVPLTRDEEAEVEAALSANRSKVLV 300

Query: 301 THDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL 360
           TH+NSNIE+T Q LQCLRPGAWLNDEVINLYLELLKERERREPEKYL CHFFNTFFYKKL
Sbjct: 301 THENSNIEITAQALQCLRPGAWLNDEVINLYLELLKERERREPEKYLNCHFFNTFFYKKL 360

Query: 361 INGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAVINKKEKKFQYLDSLK 420
           INGRDGYDYKSVRRWT+QRKLKYEL DC+KIFVPIHREIHWCLAVINKKEKKFQYLDS+K
Sbjct: 361 INGRDGYDYKSVRRWTSQRKLKYELNDCDKIFVPIHREIHWCLAVINKKEKKFQYLDSVK 420

Query: 421 GMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS 480
           GMDSRVL+TLARY+VDEVKDKSGKDIDVSSWA EFV+DLPEQ NG+DCGMFMIKY DFYS
Sbjct: 421 GMDSRVLKTLARYFVDEVKDKSGKDIDVSSWAHEFVKDLPEQFNGYDCGMFMIKYTDFYS 480

Query: 481 RGLTLCFKQEHMPYFRLRTAKEILKLIAN 501
           RGL LCFKQEHMPYFR RTAKEIL L AN
Sbjct: 481 RGLDLCFKQEHMPYFRRRTAKEILNLRAN 492

BLAST of Sed0000099 vs. ExPASy TrEMBL
Match: A0A6J1EVH3 (ubiquitin-like-specific protease ESD4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436959 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 5.5e-220
Identity = 402/509 (78.98%), Postives = 433/509 (85.07%), Query Frame = 0

Query: 1   MGARTSNRKRDDECLSVNRSYSSFKSPDFHVSKKLKFSSMSTDPPVVSSNSTAARLSRYP 60
           MGARTSNRKRDDE  S+ RSYSS KSPDFHVSKKLKFS+MS+D PVVSSNST ARLSRYP
Sbjct: 17  MGARTSNRKRDDEYSSIKRSYSSLKSPDFHVSKKLKFSTMSSDRPVVSSNSTVARLSRYP 76

Query: 61  EETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNRELSEQDKMGNILSYNYQIAKRNAIGA 120
           EETPQLRR+VHGPCR  KFGL   FFR  ES+N E SEQD +GNILSYNYQIAKRNAIGA
Sbjct: 77  EETPQLRREVHGPCRGLKFGLSRSFFRFWESKNSEPSEQDDVGNILSYNYQIAKRNAIGA 136

Query: 121 LRSFPKDVIELDSDM---------SKDDDEVEVIEDDKRERRSVSEDRRLCEVDTLQELD 180
           LRSFPKD  ELDSD          SKDD+EVEVIED                  T+QEL 
Sbjct: 137 LRSFPKDAFELDSDTQTERDASGDSKDDEEVEVIED-----------------VTVQELV 196

Query: 181 AKVVDVHQPSSSSVVVDLTSGDSKLENAEKMLGALSLRQDLSSVSAYKKLLQSVEKRTSK 240
           AK +D HQPSSSSVV+DLT  DSK+ENAEKMLG LSL +++SSVS YKKLLQ VEKRTS+
Sbjct: 197 AKKMDAHQPSSSSVVIDLTDEDSKVENAEKMLGVLSLDREISSVSTYKKLLQGVEKRTSR 256

Query: 241 LKSLDFEIELNEKRRSILQSLTPKKKLVDEIPKEIFLPLTKEEEVEVQRAFSTNRRKILV 300
           LK+LDFEIELNEKRRSILQS TPKK  VDEI +E F+PLT++EE EV+ A S NR K+LV
Sbjct: 257 LKTLDFEIELNEKRRSILQSQTPKKMPVDEIQQEPFVPLTRDEEAEVEAALSANRSKVLV 316

Query: 301 THDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKL 360
           TH+NSNIE+T Q LQCLRPGAWLNDEVINLYLELLKERERREPEKYL CHFFNTFFYKKL
Sbjct: 317 THENSNIEITAQALQCLRPGAWLNDEVINLYLELLKERERREPEKYLNCHFFNTFFYKKL 376

Query: 361 INGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAVINKKEKKFQYLDSLK 420
           INGRDGYDYKSVRRWT+QRKLKYEL DC+KIFVPIHREIHWCLAVINKKEKKFQYLDS+K
Sbjct: 377 INGRDGYDYKSVRRWTSQRKLKYELNDCDKIFVPIHREIHWCLAVINKKEKKFQYLDSVK 436

Query: 421 GMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLPEQENGFDCGMFMIKYADFYS 480
           GMDSRVL+TLARY+VDEVKDKSGKDIDVSSWA EFV+DLPEQ NG+DCGMFMIKY DFYS
Sbjct: 437 GMDSRVLKTLARYFVDEVKDKSGKDIDVSSWAHEFVKDLPEQFNGYDCGMFMIKYTDFYS 496

Query: 481 RGLTLCFKQEHMPYFRLRTAKEILKLIAN 501
           RGL LCFKQEHMPYFR RTAKEIL L AN
Sbjct: 497 RGLDLCFKQEHMPYFRRRTAKEILNLRAN 508

BLAST of Sed0000099 vs. TAIR 10
Match: AT4G15880.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 466.1 bits (1198), Expect = 3.6e-131
Identity = 272/524 (51.91%), Postives = 351/524 (66.98%), Query Frame = 0

Query: 1   MGARTSNRKRDDECLSVNRSYSS---FKSPDFHVSKKLKFS-SMSTDP-PVVSSNSTAAR 60
           MGA   NRKR DE  +     S+     SP F  SKK +FS +MS D     SSN T +R
Sbjct: 1   MGAVAINRKRSDESFNFINQQSTNPLRNSPYFQASKKRRFSFAMSEDSGKPASSNPTISR 60

Query: 61  LSRYPEETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNRELSEQDKMGNILSYNYQIAKR 120
           +SRYP+    LRR++H P R           R  ++++ +  E+D   N     Y  AKR
Sbjct: 61  ISRYPDAKAPLRREIHAPSR--------GILRYGKAKSNDYCEKD--ANFFVRKYDDAKR 120

Query: 121 NAIGALR--SFPKDVIELDSDMSK-----DDDEVEVIE----DDKRERRSVSEDRRLCEV 180
           +A+ ALR  +  KD ++L  ++ K     DD  V+ IE    DD  E++++         
Sbjct: 121 SALEALRFVNKGKDFVDLGDEVEKEEVVSDDSSVQAIEVIDCDDDEEKKNL--------- 180

Query: 181 DTLQELDAKVVDVHQPSSSSVVVDLTSGDS-KLENAEKMLGALSLRQDL----SSVSAYK 240
                         QPS SS V D+  G++ ++E+   ML +LSL +D+    SS+ AY+
Sbjct: 181 --------------QPSFSSGVTDVKKGENFRVEDTSMMLDSLSLDRDVDNDASSLEAYR 240

Query: 241 KLLQSVEKRTSKLKSLDFEIELNEKRRSILQSLTPK--KKLVDEIPKEIFLPLTKEEEVE 300
           KL+QS EKR SKL++L FEI LNEK+ S+L+   PK  +K V E+P+E F+PLT++EE E
Sbjct: 241 KLMQSAEKRNSKLEALGFEIVLNEKKLSLLRQSRPKTVEKRV-EVPREPFIPLTEDEEAE 300

Query: 301 VQRAFS-TNRRKILVTHDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEK 360
           V RAFS  NRRK+L TH+NSNI++TG++LQCL P AWLNDEVIN+YLELLKERE REP+K
Sbjct: 301 VYRAFSGRNRRKVLATHENSNIDITGEVLQCLTPSAWLNDEVINVYLELLKERETREPKK 360

Query: 361 YLKCHFFNTFFYKKLINGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAV 420
           YLKCH+FNTFFYKKL++   GY++K+VRRWTTQRKL Y LIDC+ IFVPIHR +HW LAV
Sbjct: 361 YLKCHYFNTFFYKKLVSD-SGYNFKAVRRWTTQRKLGYALIDCDMIFVPIHRGVHWTLAV 420

Query: 421 INKKEKKFQYLDSLKGMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLPEQENG 480
           IN +E K  YLDSL G+D  +L  LA+Y  DE  +KSGK ID +SW  EFVEDLP+Q+NG
Sbjct: 421 INNRESKLLYLDSLNGVDPMILNALAKYMGDEANEKSGKKIDANSWDMEFVEDLPQQKNG 480

Query: 481 FDCGMFMIKYADFYSRGLTLCFKQEHMPYFRLRTAKEILKLIAN 501
           +DCGMFM+KY DF+SRGL LCF QEHMPYFRLRTAKEIL+L A+
Sbjct: 481 YDCGMFMLKYIDFFSRGLGLCFSQEHMPYFRLRTAKEILRLRAD 489

BLAST of Sed0000099 vs. TAIR 10
Match: AT3G06910.1 (UB-like protease 1A )

HSP 1 Score: 401.4 bits (1030), Expect = 1.1e-111
Identity = 225/468 (48.08%), Postives = 301/468 (64.32%), Query Frame = 0

Query: 48  SSNSTAARLSRYPEETPQLRRQVHGPCRVPKFGLPSRFFRLSESRNRELSEQDKMGNILS 107
           SS   A  + RYPE    LRRQVH P R+   G           R+      + +G  L+
Sbjct: 61  SSRPMAPGIYRYPEVKSSLRRQVHAPVRILNSG---------RDRSTRQGSGNVLGTFLT 120

Query: 108 YNYQIAKRNAIGALRSF--PKDVIELDS-----DMSKDDDEVEVIED-----DKRERRSV 167
            N  + KRNA+ +   +   ++VI++D      +M  DD   E +E+     D+ E ++ 
Sbjct: 121 RNNDMWKRNALDSSLRYRTDREVIDVDDELGDVEMISDDTSREGVENVAMEVDEVEEKAE 180

Query: 168 SEDRRLCEVDTLQELDAKVVDVHQPSSSSVVVDLTSGDSKLENAEKMLGALSLRQDLSSV 227
             +    EV +L+    +V +  + +SSS+VV+                      D++S 
Sbjct: 181 MGNGLFSEVASLKNGSLRVGECSKANSSSLVVNRPV------------------TDVTSF 240

Query: 228 SAYKKLLQSVEKRTSKLKSLDFEIELNEKRRSILQSLT----PKKKLVDEIPKEIFLPLT 287
            AY+K+L+S   RTSKLK   F     E+ R++L+SL+      ++ V+ + +E F+PL+
Sbjct: 241 EAYRKVLESAVNRTSKLKDRGFVDFFKERGRALLRSLSSFWRQDEEPVEVVQREAFVPLS 300

Query: 288 KEEEVEVQRAFSTNRRKILVTHDNSNIELTGQILQCLRPGAWLNDEVINLYLELLKERER 347
           +EEE  V+RAFS N   ILVTH NSNI++TG+IL+CL+PG WLNDEVINLY+ LLKERE 
Sbjct: 301 REEETAVRRAFSANDSNILVTHKNSNIDITGKILRCLKPGKWLNDEVINLYMVLLKEREA 360

Query: 348 REPEKYLKCHFFNTFFYKKLINGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIH 407
           REP+K+LKCHFFNTFF+ KL+N   GY+Y +VRRWT+ ++L Y L DC+KIF+PIH  IH
Sbjct: 361 REPKKFLKCHFFNTFFFTKLVNSATGYNYGAVRRWTSMKRLGYHLKDCDKIFIPIHMNIH 420

Query: 408 WCLAVINKKEKKFQYLDSLKGMDSRVLRTLARYYVDEVKDKSGKDIDVSSWAQEFVEDLP 467
           W LAVIN K++KFQYLDS KG + ++L  LARY+VDEV+DKS  D+DVS W QEFV+DLP
Sbjct: 421 WTLAVINIKDQKFQYLDSFKGREPKILDALARYFVDEVRDKSEVDLDVSRWRQEFVQDLP 480

Query: 468 EQENGFDCGMFMIKYADFYSRGLTLCFKQEHMPYFRLRTAKEILKLIA 500
            Q NGFDCGMFM+KY DFYSRGL LCF QE MPYFR RTAKEIL+L A
Sbjct: 481 MQRNGFDCGMFMVKYIDFYSRGLDLCFTQEQMPYFRARTAKEILQLKA 501

BLAST of Sed0000099 vs. TAIR 10
Match: AT4G00690.1 (UB-like protease 1B )

HSP 1 Score: 313.2 bits (801), Expect = 3.9e-85
Identity = 172/304 (56.58%), Postives = 214/304 (70.39%), Query Frame = 0

Query: 213 SSVSAYKKLLQSVEKRTSKLKSLDFEIELNEKR-------RSILQSLTPKKKLVDEIPKE 272
           SSVS  KK  +  + +   +K  D  IE +           + L S T  KKLV     E
Sbjct: 48  SSVSLKKKAARCSQSQ-QVVKHNDDRIEEHSSSNRGFKDGNNFLVSSTETKKLV-AFCGE 107

Query: 273 IFLPLTKEEEVEVQRAFS-TNRRKILVTHDNSNIELTGQILQCLRPGAWLNDEVINLYLE 332
            F PL +EE   V  A S  NR+KILV+H NSNI+++G+ LQCLRP  WLND+V NLYLE
Sbjct: 108 PFQPLNEEEVALVNSALSKRNRKKILVSHKNSNIDISGETLQCLRPNQWLNDDVTNLYLE 167

Query: 333 LLKERERREPEKYLKCHFFNTFFYKKLINGRDGYDYKSVRRWTTQRKLKYELIDCEKIFV 392
           LLKER+ R+P+KY KCHFFNTFFY KL++G  GY+YK+V RWTT+RKL Y+LIDC+ IFV
Sbjct: 168 LLKERQTRDPQKYFKCHFFNTFFYVKLVSG-SGYNYKAVSRWTTKRKLGYDLIDCDIIFV 227

Query: 393 PIHREIHWCLAVINKKEKKFQYLDSL-KGMDSRVLRTLARYYVDEVKDKSGKDIDVSSWA 452
           PIH +IHW L VIN +E+KF YLDSL  G+   +L  +A+Y VDEVK KS K+IDVSSW 
Sbjct: 228 PIHIDIHWTLGVINNRERKFVYLDSLFTGVGHTILNAMAKYLVDEVKQKSQKNIDVSSWG 287

Query: 453 QEFVEDLPEQENGFDCGMFMIKYADFYSRGLTLCFKQ-------EHMPYFRLRTAKEILK 501
            E+VE+ P+Q+NG+DCGMFM+KY DFYSRGL+L F Q       + MPYFRLRTAKEIL+
Sbjct: 288 MEYVEERPQQQNGYDCGMFMLKYIDFYSRGLSLQFSQVIRDVIKKDMPYFRLRTAKEILR 347

BLAST of Sed0000099 vs. TAIR 10
Match: AT1G10570.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 2.4e-18
Identity = 81/289 (28.03%), Postives = 135/289 (46.71%), Query Frame = 0

Query: 245 RRSILQSLTPKKKLVDEI----PKEIFLPLTKEEEVEVQRAFSTNRRKILVTHDNSN--- 304
           + S LQS + +KK  D++      E   P+  EE  E+      +      + D S+   
Sbjct: 272 KASPLQSSSCRKKSDDKVINLDEDEPLSPMVVEEACELPEGLPED--IYYPSSDQSDGRD 331

Query: 305 -IELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKLI---- 364
            ++++ + L+CL PG +L   VIN Y+  ++       +    CHFFNTFFYKKL     
Sbjct: 332 LVQVSLKDLKCLSPGEYLTSPVINFYIRYVQHHVFSADKTAANCHFFNTFFYKKLTEAVS 391

Query: 365 ---NGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAVINKKEKKFQ---- 424
              N RD Y  K  R W       ++L     IF+PIH ++HW L +I   +K+ +    
Sbjct: 392 YKGNDRDAYFVKFRRWWK-----GFDLFCKSYIFIPIHEDLHWSLVIICIPDKEDESGLT 451

Query: 425 --YLDSLKGMDSR--VLRTLARYYVDE---VKDKSGKDIDVSS--W------AQEFVEDL 484
             +LDSL G+  R  +   + R+  +E   +   +  D+ +S+  W        E    +
Sbjct: 452 IIHLDSL-GLHPRNLIFNNVKRFLREEWNYLNQDAPLDLPISAKVWRDLPNMINEAEVQV 511

Query: 485 PEQENGFDCGMFMI----KYADFYSRGLTL-CFKQEHMPYFRLRTAKEI 495
           P+Q+N FDCG+F++    ++ +   + LTL   K  H  +F+   A  +
Sbjct: 512 PQQKNDFDCGLFLLFFIRRFIEEAPQRLTLQDLKMIHKKWFKPEEASAL 552

BLAST of Sed0000099 vs. TAIR 10
Match: AT1G10570.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 2.4e-18
Identity = 81/289 (28.03%), Postives = 135/289 (46.71%), Query Frame = 0

Query: 245 RRSILQSLTPKKKLVDEI----PKEIFLPLTKEEEVEVQRAFSTNRRKILVTHDNSN--- 304
           + S LQS + +KK  D++      E   P+  EE  E+      +      + D S+   
Sbjct: 271 KASPLQSSSCRKKSDDKVINLDEDEPLSPMVVEEACELPEGLPED--IYYPSSDQSDGRD 330

Query: 305 -IELTGQILQCLRPGAWLNDEVINLYLELLKERERREPEKYLKCHFFNTFFYKKLI---- 364
            ++++ + L+CL PG +L   VIN Y+  ++       +    CHFFNTFFYKKL     
Sbjct: 331 LVQVSLKDLKCLSPGEYLTSPVINFYIRYVQHHVFSADKTAANCHFFNTFFYKKLTEAVS 390

Query: 365 ---NGRDGYDYKSVRRWTTQRKLKYELIDCEKIFVPIHREIHWCLAVINKKEKKFQ---- 424
              N RD Y  K  R W       ++L     IF+PIH ++HW L +I   +K+ +    
Sbjct: 391 YKGNDRDAYFVKFRRWWK-----GFDLFCKSYIFIPIHEDLHWSLVIICIPDKEDESGLT 450

Query: 425 --YLDSLKGMDSR--VLRTLARYYVDE---VKDKSGKDIDVSS--W------AQEFVEDL 484
             +LDSL G+  R  +   + R+  +E   +   +  D+ +S+  W        E    +
Sbjct: 451 IIHLDSL-GLHPRNLIFNNVKRFLREEWNYLNQDAPLDLPISAKVWRDLPNMINEAEVQV 510

Query: 485 PEQENGFDCGMFMI----KYADFYSRGLTL-CFKQEHMPYFRLRTAKEI 495
           P+Q+N FDCG+F++    ++ +   + LTL   K  H  +F+   A  +
Sbjct: 511 PQQKNDFDCGLFLLFFIRRFIEEAPQRLTLQDLKMIHKKWFKPEEASAL 551