Pay0022727 (gene) Melon (Payzawat) v1

Overview


Name	Pay0022727
Type	gene
Organism	Cucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description	Protease inhibitor protein
Location	chr06: 3288128 .. 3288587 (+)
RNA-Seq Expression	Pay0022727
Synteny	Pay0022727

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGAAGAACCTGCTCAAAGTAAGTTCATCATATCTTATGCTTATTTATAATAATCTAACATGTATTTGATCAATTATAATAAGTATTTTATGTTATAAGATTTGGCTTTTGTTAATTAATTTGAAGTTTTGTATATTTATTTTGTTTGATCATGTATGTAATATGTAATTTAGAGTTATATTCAATCAATAAGGGTATACTTTGATCGGATTATCTTTGATATCTATGATAACATTTAACATAAATATAATTCTTCAAATAGGGAAAATGGAATGGCGAGAGCTTGTTTTTGTAAAATTTTCTATTGCTGCTGCGATCATAGAAAAAGAAAACCCTGATGTGAAGGCTATCAAAATCCTAGCTGGTACTCCTAGACTTCATAGTTTTGATGCTACACGAGTTTGGGTTGATTGCAATATAGAAGATGTGGTTGTTCTAATCCCTATAGTTGGTTAG

mRNA sequence

ATGGCAGAAGAACCTGCTCAAAGGAAAATGGAATGGCGAGAGCTTGTTTTTGTAAAATTTTCTATTGCTGCTGCGATCATAGAAAAAGAAAACCCTGATGTGAAGGCTATCAAAATCCTAGCTGGTACTCCTAGACTTCATAGTTTTGATGCTACACGAGTTTGGGTTGATTGCAATATAGAAGATGTGGTTGTTCTAATCCCTATAGTTGGTTAG

Coding sequence (CDS)

ATGGCAGAAGAACCTGCTCAAAGGAAAATGGAATGGCGAGAGCTTGTTTTTGTAAAATTTTCTATTGCTGCTGCGATCATAGAAAAAGAAAACCCTGATGTGAAGGCTATCAAAATCCTAGCTGGTACTCCTAGACTTCATAGTTTTGATGCTACACGAGTTTGGGTTGATTGCAATATAGAAGATGTGGTTGTTCTAATCCCTATAGTTGGTTAG

Protein sequence

MAEEPAQRKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNIEDVVVLIPIVG

Homology

[Close]

BLAST of Pay0022727 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 4.7e-05
Identity = 27/63 (42.86%), Postives = 36/63 (57.14%), Query Frame = 0


Query: 9  KMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNIEDVVVLIP 68
          K  W  LV V  S+A AIIE++NP+VKA+ +  GTP    F   RV +  N   +VV  P
Sbjct: 6  KSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSPP 65

Query: 69 IVG 72
           +G
Sbjct: 66 RIG 68

[Close]

BLAST of Pay0022727 vs. ExPASy TrEMBL
Match: A0A5A7U4U0 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001250 PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.8e-20
Identity = 52/71 (73.24%), Postives = 59/71 (83.10%), Query Frame = 0


Query: 1  MAEEPAQRKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNI 60
          MAEE AQRK  W ELVFV+FS AA IIEKENPDVKAIKILA +PR+ +FD +RVWVDCN+
Sbjct: 1  MAEESAQRKTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNV 60

Query: 61 EDVVVLIPIVG 72
          ED VV +P VG
Sbjct: 61 EDKVVKVPSVG 71

[Close]

BLAST of Pay0022727 vs. ExPASy TrEMBL
Match: A0A0A0L8E6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 9.1e-20
Identity = 52/71 (73.24%), Postives = 60/71 (84.51%), Query Frame = 0


Query: 1  MAEEPAQRKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNI 60
          MAEE A RK EW ELVFVK+SIAAAIIEKENPDV+A+KIL+GTPR+ +FD  RVWVD N+
Sbjct: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINV 60

Query: 61 EDVVVLIPIVG 72
          E+VVV  P VG
Sbjct: 61 EEVVVKKPKVG 71

[Close]

BLAST of Pay0022727 vs. ExPASy TrEMBL
Match: A0A0A0L785 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141900 PE=3 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 7.7e-19
Identity = 50/71 (70.42%), Postives = 56/71 (78.87%), Query Frame = 0


Query: 1  MAEEPAQRKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNI 60
          MAEE AQRK  W ELVFV F  AA IIEKENPDVKAIKIL  +PR+ +FD +RVWVDCNI
Sbjct: 1  MAEESAQRKTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNI 60

Query: 61 EDVVVLIPIVG 72
          E+ VV +P VG
Sbjct: 61 EERVVKVPSVG 71

[Close]

BLAST of Pay0022727 vs. ExPASy TrEMBL
Match: A0A5A7U228 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001230 PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.2e-16
Identity = 43/68 (63.24%), Postives = 57/68 (83.82%), Query Frame = 0


Query: 3  EEPAQRKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNIED 62
          ++ A RK +W ELVF+K+SIAAAIIE+ENPDV+A+KIL+GTPR+ +FD  RVW D N+ED
Sbjct: 4  DQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVED 63

Query: 63 VVVLIPIV 71
          V+V +P V
Sbjct: 64 VIVKMPKV 71

[Close]

BLAST of Pay0022727 vs. ExPASy TrEMBL
Match: A0A5A7U4L9 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001240 PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 4.7e-16
Identity = 45/69 (65.22%), Postives = 54/69 (78.26%), Query Frame = 0


Query: 3  EEPAQRKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNIED 62
          ++ A RK +W ELVFVK+S AAAIIEKENPDVKA+KIL+G  R+ +FD  RVW D N+ED
Sbjct: 4  DQSAPRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVED 63

Query: 63 VVVLIPIVG 72
          VVV  P VG
Sbjct: 64 VVVKTPKVG 72

[Close]

BLAST of Pay0022727 vs. NCBI nr
Match: KAA0049286.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 107.8 bits (268), Expect = 3.8e-20
Identity = 52/71 (73.24%), Postives = 59/71 (83.10%), Query Frame = 0


Query: 1  MAEEPAQRKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNI 60
          MAEE AQRK  W ELVFV+FS AA IIEKENPDVKAIKILA +PR+ +FD +RVWVDCN+
Sbjct: 1  MAEESAQRKTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNV 60

Query: 61 EDVVVLIPIVG 72
          ED VV +P VG
Sbjct: 61 EDKVVKVPSVG 71

[Close]

BLAST of Pay0022727 vs. NCBI nr
Match: KGN56897.1 (hypothetical protein Csa_010510 [Cucumis sativus])

HSP 1 Score: 105.5 bits (262), Expect = 1.9e-19
Identity = 52/71 (73.24%), Postives = 60/71 (84.51%), Query Frame = 0


Query: 1  MAEEPAQRKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNI 60
          MAEE A RK EW ELVFVK+SIAAAIIEKENPDV+A+KIL+GTPR+ +FD  RVWVD N+
Sbjct: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINV 60

Query: 61 EDVVVLIPIVG 72
          E+VVV  P VG
Sbjct: 61 EEVVVKKPKVG 71

[Close]

BLAST of Pay0022727 vs. NCBI nr
Match: KAE8650337.1 (hypothetical protein Csa_010581 [Cucumis sativus])

HSP 1 Score: 99.8 bits (247), Expect = 1.0e-17
Identity = 48/68 (70.59%), Postives = 54/68 (79.41%), Query Frame = 0


Query: 1  MAEEPAQRKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNI 60
          MAEE AQRK  W ELVFV F  AA IIEKENPDVKAIKIL  +PR+ +FD +RVWVDCNI
Sbjct: 1  MAEESAQRKTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNI 60

Query: 61 EDVVVLIP 69
          E+ VV +P
Sbjct: 61 EERVVKVP 68

[Close]

BLAST of Pay0022727 vs. NCBI nr
Match: KAA0049288.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 95.1 bits (235), Expect = 2.5e-16
Identity = 43/68 (63.24%), Postives = 57/68 (83.82%), Query Frame = 0


Query: 3  EEPAQRKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNIED 62
          ++ A RK +W ELVF+K+SIAAAIIE+ENPDV+A+KIL+GTPR+ +FD  RVW D N+ED
Sbjct: 4  DQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVED 63

Query: 63 VVVLIPIV 71
          V+V +P V
Sbjct: 64 VIVKMPKV 71

[Close]

BLAST of Pay0022727 vs. NCBI nr
Match: KAA0049287.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 93.2 bits (230), Expect = 9.7e-16
Identity = 45/69 (65.22%), Postives = 54/69 (78.26%), Query Frame = 0


Query: 3  EEPAQRKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNIED 62
          ++ A RK +W ELVFVK+S AAAIIEKENPDVKA+KIL+G  R+ +FD  RVW D N+ED
Sbjct: 4  DQSAPRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVED 63

Query: 63 VVVLIPIVG 72
          VVV  P VG
Sbjct: 64 VVVKTPKVG 72

[Close]

BLAST of Pay0022727 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 48.9 bits (115), Expect = 1.9e-06
Identity = 30/71 (42.25%), Postives = 41/71 (57.75%), Query Frame = 0


Query: 1  MAEEPAQRKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNI 60
          + E+P   K  W EL+  K   A  +IE+ENP +KA+ IL GT     F  +RV+V  N 
Sbjct: 31 ICEDPG--KSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVND 90

Query: 61 EDVVVLIPIVG 72
            +VV IPI+G
Sbjct: 91 CGIVVQIPIIG 99

[Close]

BLAST of Pay0022727 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 42.7 bits (99), Expect = 1.4e-04
Identity = 29/66 (43.94%), Postives = 34/66 (51.52%), Query Frame = 0


Query: 8  RKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSF--DATRVWVDCNIEDVVV 67
          RK  W EL       AA +IE+ENP V A  IL G+P    F  D  RV+VD N   +VV
Sbjct: 7  RKNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGN--RIVV 66

Query: 68 LIPIVG 72
            P  G
Sbjct: 67 KTPKSG 70

[Close]

BLAST of Pay0022727 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 41.2 bits (95), Expect = 4.1e-04
Identity = 23/64 (35.94%), Postives = 33/64 (51.56%), Query Frame = 0


Query: 8  RKMEWRELVFVKFSIAAAIIEKENPDVKAIKILAGTPRLHSFDATRVWVDCNIEDVVVLI 67
          +K  W ELV     +A   IE+ENP+V AI +  G  R+ +F   RV+V       V   
Sbjct: 22 KKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADA 81

Query: 68 PIVG 72
          P++G
Sbjct: 82 PMIG 85

The following BLAST results are available for this feature:

BLAST of Pay0022727 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	4.7e-05	42.86	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]

BLAST of Pay0022727 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U4U0	1.8e-20	73.24	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A0A0L8E6	9.1e-20	73.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1	[more]
A0A0A0L785	7.7e-19	70.42	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141900 PE=3 SV=1	[more]
A0A5A7U228	1.2e-16	63.24	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7U4L9	4.7e-16	65.22	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]

BLAST of Pay0022727 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0049286.1	3.8e-20	73.24	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhib...	[more]
KGN56897.1	1.9e-19	73.24	hypothetical protein Csa_010510 [Cucumis sativus]	[more]
KAE8650337.1	1.0e-17	70.59	hypothetical protein Csa_010581 [Cucumis sativus]	[more]
KAA0049288.1	2.5e-16	63.24	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhib...	[more]
KAA0049287.1	9.7e-16	65.22	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhib...	[more]

BLAST of Pay0022727 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43580.1	1.9e-06	42.25	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38870.1	1.4e-04	43.94	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	4.1e-04	35.94	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 9..71 e-value: 9.1E-14 score: 51.9
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 3..71
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 4..71 e-value: 7.6E-16 score: 60.2
None	No IPR available	PANTHER	PTHR33091:SF62	PROTEASE INHIBITOR PROTEIN	coord: 3..71
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 7..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Pay0022727.1	Pay0022727.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity