Pay0021435 (gene) Melon (Payzawat) v1

Overview
NamePay0021435
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Descriptionserine/threonine-protein kinase SAPK2-like
Locationchr08: 26387553 .. 26393701 (-)
RNA-Seq ExpressionPay0021435
SyntenyPay0021435
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATATGCTGCTGGGGGAGAACTCTTCAAACGAATTTACAATTCTAGTCGTTGTACTGAAGATGAGGTCTCTTATCTATCTACTCTTTCTCCCTATCTCTCGTATACATGAAATCAGTCTCTCTAGTTGTATTATTCTTGTCAAGGTTTATTCAAAACTTAATACCTCATAACACAGCCAATTACACACCCCAAATTACTCACCCAAAAGCTAAGCTTCTTCCTAGCATTATCTCGCCCACGAGAGACTGTGGTGGTTATGTTGCAAGTGTTTCTCATGAACTTTCAAGAGAAGAAATTTGTTCTTTTATAAAACTCAAAAACATAGAAGAATATTGTTATCTTGAGGCACACTTTTCTTTCTAAGCTTCAATATTCTTCCATTGATTTGATACTTTTTTCTTGCAAACAAATGAAGGAAATGAACTCTTTATATAGTACTCAATAGATACTTTCTAAAAGACGTAAAATAAATGAAGTATATGCATATATATCATTTATGTACTCGAACAATTACATGTATCTAGAAATGCTTGTATCTTGAAATTAGAAATATATCTAGGATTGTGTTATCCATAATGTATCTAGCTTCTTAATTTTTAACATGCCCCCTTAAGCTAGACTTGCCTTGACTCCGAACTTCTTTTTCATTTCCAAGAATGAGTTCGTCTTTAATGCTTTTGTGAATATGTCCGCAACTTTATCTTGAGTCTTGGAATACTTGATATATACTTCTCCATCTTTGATCAATTCTCTATTGAAATGATACTTGATCTTTATGTTTTTGCTTTTTCTATGAAAAACTGGATTCTTCGAAAGTAAAATAGATGATTGATTGTCAAGGAACATGATGATCCCTTTCTCTTGTTGACTCTTCAATTCATGTGGTACCTTTCTTATCCAAAGTGCTTGACAACAAGCTGCAGACAAAGAAACGTATTCAACTTCCATTGTCGACAATGCTACAACATCCTGCTTCTTTGATGCCCATGACTGCTCCCAAACCAATATTAAATACATACCCATGAATACTTTTGAAATAATCAATATTTTCTCCCCAATCACCTTAGCCAACAAGAACGTTATCCATATTCTTTTTATAATGGATTCCATGATCAACAACAATCCAAGAATATATCTAAGAACTCTCTTTCTAGCTTTGTAATGACTTCTCTTTGGTGATGCCTAATTTTACTCAATAAACTAACTGAGAACATAAGATCAAATCTAGTTGTAGTTAAATACATTAAACTTCCAACATGACTTCTATAAATGGTGACATCAAAAACTTCACTAACGTCATGCTTGCTTAACTTTAAACTCAGTTCCATAGAAGTACTAGCACGATAAGCATTCTCCATTTTAAAATTTATCAATAAATCTTTTGCAGATACTTCTTTTGGGAAATTGCAATCTCATTACCACTTTGTTTAACTTCAATACCAAGAAAATAATGAAGTAATCCCATAACCATCAACTCAAATATTTTTTTTCATGCTCTCTTTGAATTCTTCAATCATCATGTTTTTTTTCGTAAATATTAAATCGTCAACATATAGACAAATTAATTATCAAGAAATTACCATTTTTCGTCTTCTTTAGTGTAGAGATCATGTTCACATGGACATCTTCTCAAACAATCCTTCAAGAAAAAATTGTCAATGTGGCTGTAGCAAGCACCAGTGCTTGCTTTAGCCCATACAATAAATCGACACACCTTATTTTCTTCTTTAATCTTGGCATAACCAGGAAGATGCTCAACATGATTTATTTCAGCCTCTAAATACCCATTTAGGAATGCAAACTTTACATCCATTTGGTGTATATTAAGTTGAAGCTCATTTTTTACTGCAAGGGCTAAAAACAAATGAATAGTCTCCAAGCAAGTTAATATTGAAAAAACATTTTCATAATCCACCCCAAACTTTTGTTTGTAACCTTTTAAGACGAGTCTGACCTTGTATATTTCTTCACTTCTCCATTTTGCTCTAGTTTTATTTATAGATCCATTTGACTCCAAGAGCTTTTTTGTTTCTATTAAATTGACTAACTCTCATATATGTCTCGTTTCTTTTGATTGCATCAATCACTTGATCTGTTGCATCTTTCCATTTTTCATCTTGAATTTCTTCTTCAAACTATATAGGATCAACATATGCAAATAAAGCAAAATCAATATGTTCATCATCTAGTATCCTTTTTTAAGTATTATAGATCTCTGGAGTATTTCTTATCTTCCTTGGATTAGTTTCTTCATCACTTGTGGAGTGTGATGAAGAAGGTGAAATTAGATAGTGGTTGAGTCTCTTCAAGTTCCAAGCCTCGAGCATCTTTTTTGCCATCCATGTCAACAAGTAATGAATTCTAGTCTTCATTTGGTGCATCCCATTGCTAGACTTTTGCTTCATCAAACTTGACATCTCGACTATTAATAATTTTCCTACTTATAGGGTTGTATAATTTGTAAGCCTTGGAGTTCTCACTCTACCCAACAAAAATGCATTTGTCTAATTTGTCATCTAGCTTACCTCGTTTCTGATCTGAAATATGAGAGTAAGCAATGCACCCAAACACCCTTATAGGTGACTAACAGTTGGTTTCAATCTGTTTCATGCTTCTTGAGATGTAATACCTTGCACAGTTTTAAGTTAAAGCTCGGTTTGGAAGACAAACAGAACAAGTTACTGCGTCTCCCCAAAGTTGATTGGGAAGCTACTTGGCCTTAAACATACTTCTTGCAAATTCCATTATTATTATTCTTGTTTTCCTCTCTATAACTCCATTTTGTTTAGGAATTCTTCGAACAGTCTTATGAACTTCTTTCCCTTCCTCTATTTGAAGAAGATTGTTTGTCTCTACTCTCTAACTCTGTATTTGTTACGGCTTTGGGTCATCCATTGACTCTACCGCCACGTCCACCTCTTCTTCCACCAAATCGCCCTTTAGGGGAGGATTGCATATGAAAAATTTCTTCCGAAGGAGTAGAATCAAACAACTTCAATCTGAGCTCCTAAGATTGAAGGGATCCCATTAAGCTTTTGATGGAGAGATCAGTTGACAATATGTTCATATTTTCTAGTCATGTTTCTAGGGAATTTTTGAACCACCCTTTGATCATTAATTGTTTCTGCTTTTCATCTCATTTGATTAACAATTAAGAGAATACGGTTGAAAAATGCTTCAATAGATACAGCATCTTCCATTCAAATGGTATATATCAAATTCAGTTCAAAGTGTTCGCAATCGAACCAATTTTACTTTTTCTTCTCCGTCATACAAATTATGCAATGGATCCCATGCTGCTTTAGCACTAAAGACTCCTGATATATTCTTTCAAAAATATTTTCATCTATAGCTTGATAGATGAAAAATAATGTCTTCTTATCTTTTTTTCTAGCATCTCTCAAGTCATTGAGTTGTTGTGGTGAAGGACCATTCTCACTTTCTGGCTCCAAATATCCTTTGTCAACAATATCACAAAGATCATGAGATCCATAAAGAACCTTCATTTGTTAGTTCCACTGTCTATAGTATTTTCCCTCAAAGCGAGGAAGTTAAGGCTACAACATGTGGAGATGAATCGTTTTAATCTTCAACTTTGGTGGATACCACTCTTATTGGAAGCATTGACAAGTCCACTTTAAATCTCACAAACTTTTAAGAGAAGAAACTTGTTTTCTTACAAAACTAAAAAACACGCAATAGAAGAATATTGTTCTCTTGAAACTCATTTTCTTTCTAAGCTTGAATATTCTTTCATTGATTTGATGCTTTTTTCATGCATACAAATTAATGAAGGAAATGAACTCTTTATATAGTACTCACGAGACACTTCTTTAAGAGACACAAATAGAGTTGAAGTACTGCATAGTTATCCAATTCAAGTACTTGAACAGATTTACTGTATATCGAAGAAAATGCATGTATTTGGTTGGTTCATTTGATACATCAAGGAGAAGTAATGCAAGAGAAAATGTAGTTAAATTAATGAAATGTTTGATTATATGGTAATTTCAAACCTCACACTACAATGATCTTATTTGTTACAGGCAAGATATTTCTTCCAACAGCTGATTTGTGGACTCCAATATATTCATTCAATGGTAGGATATAATTAAAAACTAAACATTATATGGTTTATTGTTTAAAAATGTTATTATACCGTATTTTGTTTGATAATGCAAACAGGTTTATGCAACAAATATGCCATAGAGACTTGAAGCTAGATAATAGATGGAAGCAAAGCACCACGCGTGAAGATCTGTGATTTTGGCTACTCCAAGGTCGCTCTTTTAATATATAAATGATCGTGAGAGGTAAGAGTGATAATTGTATTTGCTCATTTCAAGTTAACTTGCTCTTGCTTCAAATTTGTTTTGTAGTCATCCATGCTGTATTCAAGGCTAAATGCAATTGTTGATTCTACAACGAATGCAGAACCAATGGATCTAGCAAACAGACAATATAATCGAGAGGTATCTGAGATAATCATACTGCGCAACTACATTATTGCAAAGTGATGTACTTAAATTTCTAAATTTCTTTGGTCCTATTTCAAGATATAATTATATCTGCATTGTCTTCTTCTTTTGGCATGTGTTTTAGTTGTTAGCTACATTTATGGTCACCTAAAGTTATTCTCCATTATTAAATCGACAAATAAAGATAATAACAAAAAAAAAAAATGGTGTAAACAACAGAGGATTGTCGACTTTGGTTCACCAGTAATATGTTAAGCCTAATTCACTTATGAGTATAAGGAGAACGATTTATTATAGAGAGAATAATTAAGATTATGTAAAGATGCTCTAGGTTACAAATTTTATATGATGTTAGTCAAAATCGTAAATAACATAATTATAAAAAATTTGAAGACCTATTTGTCTTAGGTATATCAGCCTTTTCTCAAGTCCCTTTGTATATGTTAATGATTGCACTCTTAATTGACGAGCTTTTCTCTAGCCTCTTTGTATTTGTTAACATTTGAACTCTTAATTGTCAGGCTTTTCTTTAGCCCGTTTGTACGGGCATGATGAGGGTGTTTGAGAGGAATAATGGTCTAGTTGAGATATCACAGTGCAACTAATGATCTTACTGATCGAATCTAGCCAAAAAAAAATGAGTGAAGACAAGACATTGCAACACTTCTTTGTCTTACGTTTTTGTATTGTCAATTTGTTCTTCAGTTATACCCGTTGCATTCAACACCAAATACAGCGGTTGGTTCTCCAACATACGCAACACCATAGGTTCTAGTTGAAGGAAAGTATGATGGAAAGGTATTTGTGATGATCAAATTGCAATTACGTTATTGCAAAGTCTACTACTAAATACTTTAAACTTTGTTTCAAGATACAATTAATATGTACATTGTCTTCTCCTGCACATGTTTTTTAGATAGCGGATGTATGGTCATGTGGGGTTACTCTCTATATTATGTTGGTTGGCTCATATTCTTTCAACGATCCGAACGATCATGAAGACGTCCAAAAAACTATTCAGGTACTTCCCAGCTTACTGCATACTACTTATTACAATAAAGTCATCATATCTTTCTCACTTCTCATTTCTTCTGCAGCGAATAATGGGTGCAGATTATAAAATACCACATAATATTAAAATATCTGAAGATTGTCGTGAACTACTCTCTCGCATCTTTGTCCGAGCCCTTGAAGGTATACTTTTCTACGACGAACTAATATATTTTTCTCATTTTCTTATTTTTGATTACTAAATACAAAATTATCTTCGTTTTATTTTATACGACATGGTTAAATGACTAAAAATATGACAAATCTTTGTTTGTAGTTAATAGACGTACAATCTCCTTATTTCTTTTCAAATTTTACCTTCATTAAGGTGTTGGGATATACTGCTTCTATTCAATAATGTAAATCTTACGATCTCGACATGATATGACAGAGAATTTTGCTGAAAGAGATTAAAAGTCACCCATGGTTCTTAAAGAAACTGGCATGGGAACTGAATGAAGGAATCCAAGCCCTCTTTTACAGGAGGGACATTCGAACCTTCTCTCATCAAAGTGATGAAGAAATCATGAAGGTCGTGGGAGAGGCGAAAAAGAAACTGCCCTCGTCGTCGACAACCATCGTGGGCTACAAATCAAAAACTGAAGAAGAAAAATAG

mRNA sequence

ATGGAATATGCTGCTGGGGGAGAACTCTTCAAACGAATTTACAATTCTAGTCGTTGTACTGAAGATGAGTCATCCATGCTGTATTCAAGGCTAAATGCAATTGTTGATTCTACAACGAATGCAGAACCAATGGATCTAGCAAACAGACAATATAATCGAGAGATAGCGGATGTATGGTCATGTGGGGTTACTCTCTATATTATGTTGGTTGGCTCATATTCTTTCAACGATCCGAACGATCATGAAGACGTCCAAAAAACTATTCAGCGAATAATGGGTGCAGATTATAAAATACCACATAATATTAAAATATCTGAAGATTGTCGTGAACTACTCTCTCGCATCTTTGTCCGAGCCCTTGAAGGTGTTGGGATATACTGCTTCTATTCAATAATGAGGGACATTCGAACCTTCTCTCATCAAAGTGATGAAGAAATCATGAAGGTCGTGGGAGAGGCGAAAAAGAAACTGCCCTCGTCGTCGACAACCATCGTGGGCTACAAATCAAAAACTGAAGAAGAAAAATAG

Coding sequence (CDS)

ATGGAATATGCTGCTGGGGGAGAACTCTTCAAACGAATTTACAATTCTAGTCGTTGTACTGAAGATGAGTCATCCATGCTGTATTCAAGGCTAAATGCAATTGTTGATTCTACAACGAATGCAGAACCAATGGATCTAGCAAACAGACAATATAATCGAGAGATAGCGGATGTATGGTCATGTGGGGTTACTCTCTATATTATGTTGGTTGGCTCATATTCTTTCAACGATCCGAACGATCATGAAGACGTCCAAAAAACTATTCAGCGAATAATGGGTGCAGATTATAAAATACCACATAATATTAAAATATCTGAAGATTGTCGTGAACTACTCTCTCGCATCTTTGTCCGAGCCCTTGAAGGTGTTGGGATATACTGCTTCTATTCAATAATGAGGGACATTCGAACCTTCTCTCATCAAAGTGATGAAGAAATCATGAAGGTCGTGGGAGAGGCGAAAAAGAAACTGCCCTCGTCGTCGACAACCATCGTGGGCTACAAATCAAAAACTGAAGAAGAAAAATAG

Protein sequence

MEYAAGGELFKRIYNSSRCTEDESSMLYSRLNAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVGSYSFNDPNDHEDVQKTIQRIMGADYKIPHNIKISEDCRELLSRIFVRALEGVGIYCFYSIMRDIRTFSHQSDEEIMKVVGEAKKKLPSSSTTIVGYKSKTEEEK
Homology
BLAST of Pay0021435 vs. ExPASy Swiss-Prot
Match: Q6ZI44 (Serine/threonine-protein kinase SAPK6 OS=Oryza sativa subsp. japonica OX=39947 GN=SAPK6 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 5.5e-31
Identity = 90/247 (36.44%), Postives = 120/247 (48.58%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           MEYAAGGELF RI ++ R +EDE                                     
Sbjct: 77  MEYAAGGELFDRICSAGRFSEDESRYFFQQLICGVSYCHFMQICHRDLKLENTLLDGSPA 136

Query: 61  ------------SSMLYSRLNAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVG 120
                       SS+L+S+  + V +     P  L+ R+Y+ ++ADVWSCGVTLY+MLVG
Sbjct: 137 PRLKICDFGYSKSSLLHSKPKSTVGTPAYIAPEVLSRREYDGKMADVWSCGVTLYVMLVG 196

Query: 121 SYSFNDPNDHEDVQKTIQRIMGADYKIPHNIKISEDCRELLSRIFV-------------- 176
           +Y F DP+D ++ +KTI RI+   YKIP  + IS+DCR+LLSRIFV              
Sbjct: 197 AYPFEDPDDPKNFRKTIGRIVSIQYKIPEYVHISQDCRQLLSRIFVANPAKRITIREIRN 256

BLAST of Pay0021435 vs. ExPASy Swiss-Prot
Match: Q7XQP4 (Serine/threonine-protein kinase SAPK7 OS=Oryza sativa subsp. japonica OX=39947 GN=SAPK7 PE=2 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 9.4e-31
Identity = 89/247 (36.03%), Postives = 119/247 (48.18%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           MEYAAGGELF RI N+ R +EDE                                     
Sbjct: 77  MEYAAGGELFDRICNAGRFSEDEARYFFQQLICGVSYCHFMQICHRDLKLENTLLDGSPA 136

Query: 61  ------------SSMLYSRLNAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVG 120
                       SS+L+S+  + V +     P  L+ R+Y+ + ADVWSCGVTLY+MLVG
Sbjct: 137 PRLKICDFGYSKSSLLHSKPKSTVGTPAYIAPEVLSRREYDGKTADVWSCGVTLYVMLVG 196

Query: 121 SYSFNDPNDHEDVQKTIQRIMGADYKIPHNIKISEDCRELLSRIFV-------------- 176
           +Y F DP+D ++ +KTI RIM   YKIP  + +S+DCR+LLSRIFV              
Sbjct: 197 AYPFEDPDDPKNFRKTIGRIMSIQYKIPEYVHVSQDCRQLLSRIFVANPAKRITIREIRN 256

BLAST of Pay0021435 vs. ExPASy Swiss-Prot
Match: Q9FFP9 (Serine/threonine-protein kinase SRK2H OS=Arabidopsis thaliana OX=3702 GN=SRK2H PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.8e-29
Identity = 87/243 (35.80%), Postives = 119/243 (48.97%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           MEYA+GGELF+RI N+ R +E E                                     
Sbjct: 77  MEYASGGELFERICNAGRFSEAEARYFFQQLICGVDYCHSLQICHRDLKLENTLLDGSPA 136

Query: 61  ------------SSMLYSRLNAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVG 120
                       SS+L+SR  + V +     P  L+ R+Y+ + ADVWSCGVTLY+MLVG
Sbjct: 137 PLLKICDFGYSKSSLLHSRPKSTVGTPAYIAPEVLSRREYDGKHADVWSCGVTLYVMLVG 196

Query: 121 SYSFNDPNDHEDVQKTIQRIMGADYKIPHNIKISEDCRELLSRIFVR------ALEGVGI 174
            Y F DP+D  + +KTIQRIM   YKIP  + IS++CR LLSRIFV        L+ +  
Sbjct: 197 GYPFEDPDDPRNFRKTIQRIMAVQYKIPDYVHISQECRHLLSRIFVTNSAKRITLKEIKK 256

BLAST of Pay0021435 vs. ExPASy Swiss-Prot
Match: P43292 (Serine/threonine-protein kinase SRK2G OS=Arabidopsis thaliana OX=3702 GN=SRK2G PE=1 SV=2)

HSP 1 Score: 127.9 bits (320), Expect = 1.1e-28
Identity = 86/244 (35.25%), Postives = 118/244 (48.36%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           MEYA+GGELF RI  + R +E E                                     
Sbjct: 77  MEYASGGELFDRICTAGRFSEAEARYFFQQLICGVDYCHSLQICHRDLKLENTLLDGSPA 136

Query: 61  ------------SSMLYSRLNAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVG 120
                       SS+L+SR  + V +     P  L+ R+Y+ + ADVWSCGVTLY+MLVG
Sbjct: 137 PLLKICDFGYSKSSILHSRPKSTVGTPAYIAPEVLSRREYDGKHADVWSCGVTLYVMLVG 196

Query: 121 SYSFNDPNDHEDVQKTIQRIMGADYKIPHNIKISEDCRELLSRIFVR------ALEGVGI 176
           +Y F DPND ++ +KTIQRIM   YKIP  + IS++C+ LLSRIFV        L+ +  
Sbjct: 197 AYPFEDPNDPKNFRKTIQRIMAVQYKIPDYVHISQECKHLLSRIFVTNSAKRITLKEIKN 256

BLAST of Pay0021435 vs. ExPASy Swiss-Prot
Match: Q5N942 (Serine/threonine-protein kinase SAPK4 OS=Oryza sativa subsp. japonica OX=39947 GN=SAPK4 PE=2 SV=2)

HSP 1 Score: 126.7 bits (317), Expect = 2.6e-28
Identity = 84/247 (34.01%), Postives = 115/247 (46.56%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           ME+AAGGELF RI +  R +EDE                                     
Sbjct: 77  MEFAAGGELFDRICDRGRFSEDEARYFFQQLICGVSYCHHMQICHRDLKLENVLLDGSPA 136

Query: 61  ------------SSMLYSRLNAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVG 120
                       SS+L+SR  + V +     P  L+ R+Y+ ++ADVWSCGVTLY+MLVG
Sbjct: 137 PRLKICDFGYSKSSVLHSRPKSAVGTPAYIAPEVLSRREYDGKLADVWSCGVTLYVMLVG 196

Query: 121 SYSFNDPNDHEDVQKTIQRIMGADYKIPHNIKISEDCRELLSRIFV-------------- 176
           +Y F D +D ++++KTIQRIM   YKIP  + IS +C++L++RIFV              
Sbjct: 197 AYPFEDQDDPKNIRKTIQRIMSVQYKIPDYVHISAECKQLIARIFVNNPLRRITMKEIKS 256

BLAST of Pay0021435 vs. ExPASy TrEMBL
Match: A0A1S3B5L7 (serine/threonine-protein kinase SAPK2-like OS=Cucumis melo OX=3656 GN=LOC103486039 PE=3 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.8e-46
Identity = 125/261 (47.89%), Postives = 136/261 (52.11%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           MEYAAGGELFKRI  S RCTEDE                                     
Sbjct: 162 MEYAAGGELFKRISISGRCTEDETRYFFQQLICGLEYIHSRQICHRDLKLDNILLDGSKA 221

Query: 61  ------------SSMLYSRLNAIVDSTTNAEPMDLANRQYNRE----------------- 120
                       SSMLY R N IVDSTTNA PMD ANRQY+RE                 
Sbjct: 222 PRVKICDFGYSKSSMLYLRPNTIVDSTTNAAPMDPANRQYDRELYSLHSTPNTAVGSPTY 281

Query: 121 --------------IADVWSCGVTLYIMLVGSYSFNDPNDHEDVQKTIQRIMGADYKIPH 160
                         IAD+WSCGVTLYIMLVGSY F+ PNDHEDVQKTIQRI   DY IPH
Sbjct: 282 AAPEVLDEGEYDGQIADIWSCGVTLYIMLVGSYPFDHPNDHEDVQKTIQRIKNVDYNIPH 341

BLAST of Pay0021435 vs. ExPASy TrEMBL
Match: A0A5D3C0E9 (Serine/threonine-protein kinase SRK2H-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G00680 PE=3 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 4.1e-37
Identity = 109/256 (42.58%), Postives = 125/256 (48.83%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDESSMLYSRL----------------------------- 60
           MEYAAGGELFKRI  S RCTEDE+   + +L                             
Sbjct: 77  MEYAAGGELFKRISISGRCTEDETRYFFQQLICGLEYIHSRQICHRDLKLDNILLDGSKA 136

Query: 61  --------------------NAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVG 120
                               N  V S T A P  L   +Y+ +IAD+WSCGVTLYIMLVG
Sbjct: 137 PRVKICDFGYSKLYSLHSTPNTAVGSPTYAAPEVLDEGEYDGQIADIWSCGVTLYIMLVG 196

Query: 121 SYSFNDPNDHEDVQKTI--------------------------QRIMGADYKIPHNIKIS 160
           SY F+ PNDHEDVQKTI                          QRI   DY IPHNIKIS
Sbjct: 197 SYPFDHPNDHEDVQKTIQVLPSLLHTNYYNKVIISFSLLIVLLQRIKNVDYNIPHNIKIS 256

BLAST of Pay0021435 vs. ExPASy TrEMBL
Match: A0A5A7TJY2 (Serine/threonine-protein kinase SAPK2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67G002590 PE=4 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 4.1e-37
Identity = 109/256 (42.58%), Postives = 125/256 (48.83%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDESSMLYSRL----------------------------- 60
           MEYAAGGELFKRI  S RCTEDE+   + +L                             
Sbjct: 170 MEYAAGGELFKRISISGRCTEDETRYFFQQLICGLEYIHSRQICHRDLKLDNILLDGSKA 229

Query: 61  --------------------NAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVG 120
                               N  V S T A P  L   +Y+ +IAD+WSCGVTLYIMLVG
Sbjct: 230 PRVKICDFGYSKLYSLHSTPNTAVGSPTYAAPEVLDEGEYDGQIADIWSCGVTLYIMLVG 289

Query: 121 SYSFNDPNDHEDVQKTI--------------------------QRIMGADYKIPHNIKIS 160
           SY F+ PNDHEDVQKTI                          QRI   DY IPHNIKIS
Sbjct: 290 SYPFDHPNDHEDVQKTIQVLPSLLHTNYYNKVIISFSLLIVLLQRIKNVDYNIPHNIKIS 349

BLAST of Pay0021435 vs. ExPASy TrEMBL
Match: A0A1S3C2K4 (serine/threonine-protein kinase SRK2H-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496177 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 2.1e-33
Identity = 102/279 (36.56%), Postives = 128/279 (45.88%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           MEYAAGGELF+RIY+  R +EDE                                     
Sbjct: 77  MEYAAGGELFERIYHGGRFSEDEARYFFQQLICGVDYFHSLEICHRDLKLDNILLDGFPA 136

Query: 61  ------------SSMLYSRLNAIVDSTTNAEPMDLANR---------------------- 120
                       SSMLYS+ N  V STT A P DL                         
Sbjct: 137 PRLKICDFGFSKSSMLYSKPNINVGSTTYAAPEDLPKEQHDGKLYPLHSTPNTAVGSPTY 196

Query: 121 ---------QYNREIADVWSCGVTLYIMLVGSYSFNDPNDHEDVQKTIQRIMGADYKIPH 175
                    QY+ ++AD+WSCGVTLY+MLVG+Y F DPND ++  KT+ RIM   YKIP 
Sbjct: 197 SAPEVLVEGQYDGKVADIWSCGVTLYVMLVGAYPFEDPNDSKNFHKTVTRIMAVQYKIPD 256

BLAST of Pay0021435 vs. ExPASy TrEMBL
Match: A0A6V7QXZ4 (Protein kinase domain-containing protein OS=Ananas comosus var. bracteatus OX=296719 GN=CB5_LOCUS30817 PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 3.0e-32
Identity = 93/228 (40.79%), Postives = 120/228 (52.63%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE-------------------------------SSMLYS 60
           MEYAAGGELF RI N+ R +EDE                               SS+L+S
Sbjct: 115 MEYAAGGELFDRICNNGRFSEDEQICHRDLKLENTLLDGNPAPRLKICDFGYSKSSLLHS 174

Query: 61  RLNAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVGSYSFNDPNDHEDVQKTIQ 120
           R  + V +     P  L+ R+Y+ ++ADVWSCGVTLY+MLVG+Y F DP+D +D +KTI 
Sbjct: 175 RPKSTVGTPAYIAPEVLSRREYDGKLADVWSCGVTLYVMLVGAYPFEDPDDPKDFRKTIG 234

Query: 121 RIMGADYKIPHNIKISEDCRELLSRIFV-----------------------RALEGVGIY 175
           RIM   YKIP  + IS+DCR+LLSRIFV                       R L      
Sbjct: 235 RIMSVQYKIPDYVHISQDCRQLLSRIFVTNPARRITIREIRNHPWFLKNLSRDLTEAAQA 294

BLAST of Pay0021435 vs. NCBI nr
Match: XP_008442071.2 (PREDICTED: serine/threonine-protein kinase SAPK2-like [Cucumis melo])

HSP 1 Score: 194.9 bits (494), Expect = 5.8e-46
Identity = 125/261 (47.89%), Postives = 136/261 (52.11%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           MEYAAGGELFKRI  S RCTEDE                                     
Sbjct: 162 MEYAAGGELFKRISISGRCTEDETRYFFQQLICGLEYIHSRQICHRDLKLDNILLDGSKA 221

Query: 61  ------------SSMLYSRLNAIVDSTTNAEPMDLANRQYNRE----------------- 120
                       SSMLY R N IVDSTTNA PMD ANRQY+RE                 
Sbjct: 222 PRVKICDFGYSKSSMLYLRPNTIVDSTTNAAPMDPANRQYDRELYSLHSTPNTAVGSPTY 281

Query: 121 --------------IADVWSCGVTLYIMLVGSYSFNDPNDHEDVQKTIQRIMGADYKIPH 160
                         IAD+WSCGVTLYIMLVGSY F+ PNDHEDVQKTIQRI   DY IPH
Sbjct: 282 AAPEVLDEGEYDGQIADIWSCGVTLYIMLVGSYPFDHPNDHEDVQKTIQRIKNVDYNIPH 341

BLAST of Pay0021435 vs. NCBI nr
Match: XP_031740794.1 (serine/threonine-protein kinase SAPK7 isoform X1 [Cucumis sativus])

HSP 1 Score: 187.2 bits (474), Expect = 1.2e-43
Identity = 124/277 (44.77%), Postives = 144/277 (51.99%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           MEYA GG+LF+RI NS  CTEDE                                     
Sbjct: 95  MEYAGGGQLFRRISNSGCCTEDEARYFFQQLICGLEYIHSMQICHRDLKLDNILLDGSKA 154

Query: 61  ------------SSMLYSRLNAIVD-STTNAEPMDLAN---------------------- 120
                       SSMLYS+ N IVD STT A PMD AN                      
Sbjct: 155 PRVKICDFGYSKSSMLYSKPNTIVDYSTTYAAPMDQANGQDDGQLYPLHSIPNTRVGSTT 214

Query: 121 ---------RQYNREIADVWSCGVTLYIMLVGSYSFNDPNDHEDVQKTIQRIMGADYKIP 176
                     QYN +IADVWSCGVTLY+ML GSY F DPND ++++KTI+RIM  DYKIP
Sbjct: 215 YSAPEVLVEGQYNGKIADVWSCGVTLYVMLFGSYPFEDPNDPKNIRKTIKRIMHIDYKIP 274

BLAST of Pay0021435 vs. NCBI nr
Match: KAE8649702.1 (hypothetical protein Csa_012923 [Cucumis sativus])

HSP 1 Score: 172.9 bits (437), Expect = 2.4e-39
Identity = 97/170 (57.06%), Postives = 116/170 (68.24%), Query Frame = 0

Query: 27  LYSRLNAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVGSYSFNDPNDHEDVQK 86
           L+S  N  V STT + P  L   QYN +IADVWSCGVTLY+ML GSY F DPND ++++K
Sbjct: 301 LHSIPNTRVGSTTYSAPEVLVEGQYNGKIADVWSCGVTLYVMLFGSYPFEDPNDPKNIRK 360

Query: 87  TIQRIMGADYKIPHNIKISEDCRELLSRIFV------RALEGVGIYCFYS---------- 146
           TI+RIM  DYKIP NIKISEDCR+LLS IFV        L+ +  + ++           
Sbjct: 361 TIKRIMHIDYKIPENIKISEDCRDLLSHIFVVDPSMRIPLQEIKSHPWFEKKLAWELRKE 420

Query: 147 -----IMRDIRTFSHQSDEEIMKVVGEAKKKLPSSSTTIVGYKSKTEEEK 176
                 MR+I TFSHQS EEIMKVVGEAKKKLPSSSTTIV Y S+TE+E+
Sbjct: 421 IQALFYMRNIPTFSHQSVEEIMKVVGEAKKKLPSSSTTIVDYNSETEKEE 470

BLAST of Pay0021435 vs. NCBI nr
Match: KAA0041871.1 (serine/threonine-protein kinase SAPK2-like [Cucumis melo var. makuwa])

HSP 1 Score: 164.5 bits (415), Expect = 8.4e-37
Identity = 109/256 (42.58%), Postives = 125/256 (48.83%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDESSMLYSRL----------------------------- 60
           MEYAAGGELFKRI  S RCTEDE+   + +L                             
Sbjct: 170 MEYAAGGELFKRISISGRCTEDETRYFFQQLICGLEYIHSRQICHRDLKLDNILLDGSKA 229

Query: 61  --------------------NAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVG 120
                               N  V S T A P  L   +Y+ +IAD+WSCGVTLYIMLVG
Sbjct: 230 PRVKICDFGYSKLYSLHSTPNTAVGSPTYAAPEVLDEGEYDGQIADIWSCGVTLYIMLVG 289

Query: 121 SYSFNDPNDHEDVQKTI--------------------------QRIMGADYKIPHNIKIS 160
           SY F+ PNDHEDVQKTI                          QRI   DY IPHNIKIS
Sbjct: 290 SYPFDHPNDHEDVQKTIQVLPSLLHTNYYNKVIISFSLLIVLLQRIKNVDYNIPHNIKIS 349

BLAST of Pay0021435 vs. NCBI nr
Match: TYK05391.1 (serine/threonine-protein kinase SRK2H-like [Cucumis melo var. makuwa])

HSP 1 Score: 164.5 bits (415), Expect = 8.4e-37
Identity = 109/256 (42.58%), Postives = 125/256 (48.83%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDESSMLYSRL----------------------------- 60
           MEYAAGGELFKRI  S RCTEDE+   + +L                             
Sbjct: 77  MEYAAGGELFKRISISGRCTEDETRYFFQQLICGLEYIHSRQICHRDLKLDNILLDGSKA 136

Query: 61  --------------------NAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVG 120
                               N  V S T A P  L   +Y+ +IAD+WSCGVTLYIMLVG
Sbjct: 137 PRVKICDFGYSKLYSLHSTPNTAVGSPTYAAPEVLDEGEYDGQIADIWSCGVTLYIMLVG 196

Query: 121 SYSFNDPNDHEDVQKTI--------------------------QRIMGADYKIPHNIKIS 160
           SY F+ PNDHEDVQKTI                          QRI   DY IPHNIKIS
Sbjct: 197 SYPFDHPNDHEDVQKTIQVLPSLLHTNYYNKVIISFSLLIVLLQRIKNVDYNIPHNIKIS 256

BLAST of Pay0021435 vs. TAIR 10
Match: AT5G63650.1 (SNF1-related protein kinase 2.5 )

HSP 1 Score: 130.6 bits (327), Expect = 1.3e-30
Identity = 87/243 (35.80%), Postives = 119/243 (48.97%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           MEYA+GGELF+RI N+ R +E E                                     
Sbjct: 77  MEYASGGELFERICNAGRFSEAEARYFFQQLICGVDYCHSLQICHRDLKLENTLLDGSPA 136

Query: 61  ------------SSMLYSRLNAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVG 120
                       SS+L+SR  + V +     P  L+ R+Y+ + ADVWSCGVTLY+MLVG
Sbjct: 137 PLLKICDFGYSKSSLLHSRPKSTVGTPAYIAPEVLSRREYDGKHADVWSCGVTLYVMLVG 196

Query: 121 SYSFNDPNDHEDVQKTIQRIMGADYKIPHNIKISEDCRELLSRIFVR------ALEGVGI 174
            Y F DP+D  + +KTIQRIM   YKIP  + IS++CR LLSRIFV        L+ +  
Sbjct: 197 GYPFEDPDDPRNFRKTIQRIMAVQYKIPDYVHISQECRHLLSRIFVTNSAKRITLKEIKK 256

BLAST of Pay0021435 vs. TAIR 10
Match: AT5G08590.1 (SNF1-related protein kinase 2.1 )

HSP 1 Score: 127.9 bits (320), Expect = 8.1e-30
Identity = 86/244 (35.25%), Postives = 118/244 (48.36%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           MEYA+GGELF RI  + R +E E                                     
Sbjct: 77  MEYASGGELFDRICTAGRFSEAEARYFFQQLICGVDYCHSLQICHRDLKLENTLLDGSPA 136

Query: 61  ------------SSMLYSRLNAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVG 120
                       SS+L+SR  + V +     P  L+ R+Y+ + ADVWSCGVTLY+MLVG
Sbjct: 137 PLLKICDFGYSKSSILHSRPKSTVGTPAYIAPEVLSRREYDGKHADVWSCGVTLYVMLVG 196

Query: 121 SYSFNDPNDHEDVQKTIQRIMGADYKIPHNIKISEDCRELLSRIFVR------ALEGVGI 176
           +Y F DPND ++ +KTIQRIM   YKIP  + IS++C+ LLSRIFV        L+ +  
Sbjct: 197 AYPFEDPNDPKNFRKTIQRIMAVQYKIPDYVHISQECKHLLSRIFVTNSAKRITLKEIKN 256

BLAST of Pay0021435 vs. TAIR 10
Match: AT1G10940.1 (Protein kinase superfamily protein )

HSP 1 Score: 126.3 bits (316), Expect = 2.4e-29
Identity = 83/230 (36.09%), Postives = 116/230 (50.43%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           MEYAAGGELF+RI ++ R +EDE                                     
Sbjct: 77  MEYAAGGELFERICSAGRFSEDEARYFFQQLISGVSYCHAMQICHRDLKLENTLLDGSPA 136

Query: 61  ------------SSMLYSRLNAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVG 120
                       SS+L+SR  + V +     P  L+ R+Y+ ++ADVWSCGVTLY+MLVG
Sbjct: 137 PRLKICDFGYSKSSLLHSRPKSTVGTPAYIAPEVLSRREYDGKMADVWSCGVTLYVMLVG 196

Query: 121 SYSFNDPNDHEDVQKTIQRIMGADYKIPHNIKISEDCRELLSRIFV-RALEGVGI----- 161
           +Y F D  D ++ +KTIQ+IM   YKIP  + IS+DC+ LLSRIFV  +L+ + I     
Sbjct: 197 AYPFEDQEDPKNFRKTIQKIMAVQYKIPDYVHISQDCKNLLSRIFVANSLKRITIAEIKK 256

BLAST of Pay0021435 vs. TAIR 10
Match: AT1G10940.2 (Protein kinase superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 2.0e-28
Identity = 83/238 (34.87%), Postives = 116/238 (48.74%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           MEYAAGGELF+RI ++ R +EDE                                     
Sbjct: 77  MEYAAGGELFERICSAGRFSEDEEEGNKRKHARYFFQQLISGVSYCHAMQICHRDLKLEN 136

Query: 61  --------------------SSMLYSRLNAIVDSTTNAEPMDLANRQYNREIADVWSCGV 120
                               SS+L+SR  + V +     P  L+ R+Y+ ++ADVWSCGV
Sbjct: 137 TLLDGSPAPRLKICDFGYSKSSLLHSRPKSTVGTPAYIAPEVLSRREYDGKMADVWSCGV 196

Query: 121 TLYIMLVGSYSFNDPNDHEDVQKTIQRIMGADYKIPHNIKISEDCRELLSRIFV-RALEG 161
           TLY+MLVG+Y F D  D ++ +KTIQ+IM   YKIP  + IS+DC+ LLSRIFV  +L+ 
Sbjct: 197 TLYVMLVGAYPFEDQEDPKNFRKTIQKIMAVQYKIPDYVHISQDCKNLLSRIFVANSLKR 256

BLAST of Pay0021435 vs. TAIR 10
Match: AT1G60940.1 (SNF1-related protein kinase 2.10 )

HSP 1 Score: 122.1 bits (305), Expect = 4.5e-28
Identity = 81/226 (35.84%), Postives = 108/226 (47.79%), Query Frame = 0

Query: 1   MEYAAGGELFKRIYNSSRCTEDE------------------------------------- 60
           MEYAAGGELF+RI ++ R +EDE                                     
Sbjct: 77  MEYAAGGELFERICSAGRFSEDEARYFFQQLISGVSYCHAMQICHRDLKLENTLLDGSPA 136

Query: 61  ------------SSMLYSRLNAIVDSTTNAEPMDLANRQYNREIADVWSCGVTLYIMLVG 120
                       SS+L+S   + V +     P  L+  +Y+ ++ADVWSCGVTLY+MLVG
Sbjct: 137 PRLKICDFGYSKSSLLHSMPKSTVGTPAYIAPEVLSRGEYDGKMADVWSCGVTLYVMLVG 196

Query: 121 SYSFNDPNDHEDVQKTIQRIMGADYKIPHNIKISEDCRELLSRIFV-------------- 155
           +Y F D  D ++ +KTIQRIM   YKIP  + IS+DC+ LLSRIFV              
Sbjct: 197 AYPFEDQEDPKNFKKTIQRIMAVKYKIPDYVHISQDCKHLLSRIFVTNSNKRITIGDIKK 256

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6ZI445.5e-3136.44Serine/threonine-protein kinase SAPK6 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q7XQP49.4e-3136.03Serine/threonine-protein kinase SAPK7 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9FFP91.8e-2935.80Serine/threonine-protein kinase SRK2H OS=Arabidopsis thaliana OX=3702 GN=SRK2H P... [more]
P432921.1e-2835.25Serine/threonine-protein kinase SRK2G OS=Arabidopsis thaliana OX=3702 GN=SRK2G P... [more]
Q5N9422.6e-2834.01Serine/threonine-protein kinase SAPK4 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Match NameE-valueIdentityDescription
A0A1S3B5L72.8e-4647.89serine/threonine-protein kinase SAPK2-like OS=Cucumis melo OX=3656 GN=LOC1034860... [more]
A0A5D3C0E94.1e-3742.58Serine/threonine-protein kinase SRK2H-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A5A7TJY24.1e-3742.58Serine/threonine-protein kinase SAPK2-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3C2K42.1e-3336.56serine/threonine-protein kinase SRK2H-like isoform X1 OS=Cucumis melo OX=3656 GN... [more]
A0A6V7QXZ43.0e-3240.79Protein kinase domain-containing protein OS=Ananas comosus var. bracteatus OX=29... [more]
Match NameE-valueIdentityDescription
XP_008442071.25.8e-4647.89PREDICTED: serine/threonine-protein kinase SAPK2-like [Cucumis melo][more]
XP_031740794.11.2e-4344.77serine/threonine-protein kinase SAPK7 isoform X1 [Cucumis sativus][more]
KAE8649702.12.4e-3957.06hypothetical protein Csa_012923 [Cucumis sativus][more]
KAA0041871.18.4e-3742.58serine/threonine-protein kinase SAPK2-like [Cucumis melo var. makuwa][more]
TYK05391.18.4e-3742.58serine/threonine-protein kinase SRK2H-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT5G63650.11.3e-3035.80SNF1-related protein kinase 2.5 [more]
AT5G08590.18.1e-3035.25SNF1-related protein kinase 2.1 [more]
AT1G10940.12.4e-2936.09Protein kinase superfamily protein [more]
AT1G10940.22.0e-2834.87Protein kinase superfamily protein [more]
AT1G60940.14.5e-2835.84SNF1-related protein kinase 2.10 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 46..116
e-value: 1.4E-8
score: 34.5
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 9..121
e-value: 7.0E-15
score: 57.0
NoneNo IPR availablePANTHERPTHR24343SERINE/THREONINE KINASEcoord: 24..118
NoneNo IPR availablePANTHERPTHR24343SERINE/THREONINE KINASEcoord: 130..174
NoneNo IPR availablePANTHERPTHR24343:SF437SERINE/THREONINE-PROTEIN KINASE SRK2Hcoord: 130..174
coord: 24..118
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 1..118

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0021435.1Pay0021435.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity