Pay0021433 (gene) Melon (Payzawat) v1

Overview
NamePay0021433
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionRibonuclease H
Locationchr03: 4783034 .. 4783551 (+)
RNA-Seq ExpressionPay0021433
SyntenyPay0021433
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCCTATAAAGTACATTTTTGAAAAGCCATCCTTGTCTGGACGAATAGCTAAATGGCAGGTGTTGCCATCCGAGTATGATATCATCTATGTTACAAGAAATGTCGTGAAAGGAAGTGCAATTACCGATTGTTTAGCTGATTTGCCAGTCGAAGACTATGAACCAATGAAATTTGAATTTCTAGATGAGGACGTCATGACAGTATCAGATACACCTCAAGATACAGATATATGGACAATGTTCTTTGATGGAGCCACAAATAAGGTAGGACATGGTGTGGGGGCTATTCTAACATTTCCTAATGGAAAATCGTATCCTCTAACTGCTAAGTTGTACTTTAATTGCACAAATAACATGGCTAATATGAAACATGTAGTATGGGAGTTAAAATGGCCTATGACTTGAAGATTAAGAAGTTGCAAGTTTATGAAGATTCTCTCTTAGTAATACATCAGCTCAATAGGGAATGGGAGACAAGAGACTCTAAATTGATTCCCTACAACAAGTATATTTGA

mRNA sequence

ATGGATCCTATAAAGTACATTTTTGAAAAGCCATCCTTGTCTGGACGAATAGCTAAATGGCAGGTGTTGCCATCCGAGTATGATATCATCTATGTTACAAGAAATGTCGTGAAAGGAAGTGCAATTACCGATTGTTTAGCTGATTTGCCAGTCGAAGACTATGAACCAATGAAATTTGAATTTCTAGATGAGGACGTCATGACAGTATCAGATACACCTCAAGATACAGATATATGGACAATGTTCTTTGATGGAGCCACAAATAAGGTAGGACATGGTGTGGGGGCTATTCTAACATTTCCTAATGGAAAATCGTATCCTCTAACTGCTAAGTTGTACTTTAATTGCACAAATAACATGGCTAATATGAAACATATTAAGAAGTTGCAAGTTTATGAAGATTCTCTCTTAGTAATACATCAGCTCAATAGGGAATGGGAGACAAGAGACTCTAAATTGATTCCCTACAACAAGTATATTTGA

Coding sequence (CDS)

ATGGATCCTATAAAGTACATTTTTGAAAAGCCATCCTTGTCTGGACGAATAGCTAAATGGCAGGTGTTGCCATCCGAGTATGATATCATCTATGTTACAAGAAATGTCGTGAAAGGAAGTGCAATTACCGATTGTTTAGCTGATTTGCCAGTCGAAGACTATGAACCAATGAAATTTGAATTTCTAGATGAGGACGTCATGACAGTATCAGATACACCTCAAGATACAGATATATGGACAATGTTCTTTGATGGAGCCACAAATAAGGTAGGACATGGTGTGGGGGCTATTCTAACATTTCCTAATGGAAAATCGTATCCTCTAACTGCTAAGTTGTACTTTAATTGCACAAATAACATGGCTAATATGAAACATATTAAGAAGTTGCAAGTTTATGAAGATTCTCTCTTAGTAATACATCAGCTCAATAGGGAATGGGAGACAAGAGACTCTAAATTGATTCCCTACAACAAGTATATTTGA

Protein sequence

MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYEPMKFEFLDEDVMTVSDTPQDTDIWTMFFDGATNKVGHGVGAILTFPNGKSYPLTAKLYFNCTNNMANMKHIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI
Homology
BLAST of Pay0021433 vs. ExPASy TrEMBL
Match: A0A5A7UKV4 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1163G00350 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 6.1e-80
Identity = 151/172 (87.79%), Postives = 156/172 (90.70%), Query Frame = 0

Query: 1   MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYEPMKFE 60
           MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYE MKFE
Sbjct: 1   MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYELMKFE 60

Query: 61  FLDEDVMTVSDTPQDTDIWTMFFDGATNKVGHGVGAILTFPNGKSYPLTAKLYFNCTNNM 120
           FLDEDVMTVSDTPQDT+IWTMFFDGATN+VGHGVGAILTFP+GKSYPLTAKLYF+CTNNM
Sbjct: 61  FLDEDVMTVSDTPQDTEIWTMFFDGATNEVGHGVGAILTFPDGKSYPLTAKLYFDCTNNM 120

Query: 121 ANMK------------HIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 161
           A  +             IKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI
Sbjct: 121 AEYETCSMGVKMAYDLKIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 172

BLAST of Pay0021433 vs. ExPASy TrEMBL
Match: A0A1S4DTT9 (uncharacterized protein LOC107990505 OS=Cucumis melo OX=3656 GN=LOC107990505 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 6.1e-80
Identity = 151/172 (87.79%), Postives = 156/172 (90.70%), Query Frame = 0

Query: 1   MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYEPMKFE 60
           MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYE MKFE
Sbjct: 1   MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYELMKFE 60

Query: 61  FLDEDVMTVSDTPQDTDIWTMFFDGATNKVGHGVGAILTFPNGKSYPLTAKLYFNCTNNM 120
           FLDEDVMTVSDTPQDT+IWTMFFDGATN+VGHGVGAILTFP+GKSYPLTAKLYF+CTNNM
Sbjct: 61  FLDEDVMTVSDTPQDTEIWTMFFDGATNEVGHGVGAILTFPDGKSYPLTAKLYFDCTNNM 120

Query: 121 ANMK------------HIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 161
           A  +             IKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI
Sbjct: 121 AEYETCSMGVKMAYDLKIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 172

BLAST of Pay0021433 vs. ExPASy TrEMBL
Match: A0A5D3BPI8 (RNase H domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49G00050 PE=4 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 7.2e-65
Identity = 129/172 (75.00%), Postives = 138/172 (80.23%), Query Frame = 0

Query: 1   MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYEPMKFE 60
           MDPIKYIFEK SL GRIAKWQVL  EYDIIYVTR  +KGSAI DCLA+ PVEDYEPMK E
Sbjct: 64  MDPIKYIFEKSSLFGRIAKWQVLLLEYDIIYVTRKAMKGSAIVDCLANFPVEDYEPMKIE 123

Query: 61  FLDEDVMTVSDTPQDTDIWTMFFDGATNKVGHGVGAILTFPNGKSYPLTAKLYFNCTNNM 120
           F DEDVM VSDT QDT+IWTMFFDGATN+VGHGVGAILT  +GKSYPLT KLYF+CT+NM
Sbjct: 124 FPDEDVMIVSDTLQDTEIWTMFFDGATNEVGHGVGAILTSSDGKSYPLTTKLYFDCTHNM 183

Query: 121 ANMK------------HIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 161
           A  +             IKKLQVY DSLL IHQLN EWET DSKLIPY+KYI
Sbjct: 184 AEYEACSMGVQMAYDMKIKKLQVYRDSLLAIHQLNGEWETIDSKLIPYDKYI 235

BLAST of Pay0021433 vs. ExPASy TrEMBL
Match: A0A1S3BH42 (uncharacterized protein LOC103489784 OS=Cucumis melo OX=3656 GN=LOC103489784 PE=4 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.5e-54
Identity = 107/172 (62.21%), Postives = 126/172 (73.26%), Query Frame = 0

Query: 1   MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYEPMKFE 60
           MDPIKYIFEKPSLS +IAKW VL  E+DI+Y+TR  +KGS I DCL +LP+EDYEPMKF+
Sbjct: 64  MDPIKYIFEKPSLSEKIAKWHVLLLEFDIVYITRKAIKGSVIVDCLVELPIEDYEPMKFD 123

Query: 61  FLDEDVMTVSDTPQDTDIWTMFFDGATNKVGHGVGAILTFPNGKSYPLTAKLYFNCTNNM 120
           FLDED+MT+ +T  DT  WTM FD ATNKVGHG+G  L  P+ K YP T KLYF+CTNNM
Sbjct: 124 FLDEDIMTIVNTSSDT--WTMMFDRATNKVGHGMGVTLMSPDEKRYPFTTKLYFDCTNNM 183

Query: 121 ANMK------------HIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 161
              K             IK L+V  DSLLV+H+LN E+ETRD+K IPYN YI
Sbjct: 184 VEYKACSMGVRIAYEMKIKNLRVLGDSLLVVHKLNGEYETRDAKFIPYNDYI 233

BLAST of Pay0021433 vs. ExPASy TrEMBL
Match: A0A6P5WLA5 (Ribonuclease H OS=Durio zibethinus OX=66656 GN=LOC111275425 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 4.1e-52
Identity = 105/172 (61.05%), Postives = 125/172 (72.67%), Query Frame = 0

Query: 1    MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYEPMKFE 60
            +DPIKYIFEKPSL+GRIA+WQV+ SEYDIIYVT+  +KGSAI + LAD  VEDYEPMK +
Sbjct: 1337 LDPIKYIFEKPSLTGRIARWQVMLSEYDIIYVTQKAIKGSAIAEFLADRAVEDYEPMKLD 1396

Query: 61   FLDEDVMTVSDTPQDTDIWTMFFDGATNKVGHGVGAILTFPNGKSYPLTAKLYFNCTNNM 120
            FLDEDVM + +       W M+FDGA N +GHG+GAIL  P+G  YP+TA+L FNCTNN+
Sbjct: 1397 FLDEDVMAIEENEPVEKSWRMYFDGAANALGHGIGAILISPDGHYYPITARLNFNCTNNV 1456

Query: 121  ANM------------KHIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 161
            A              K IK L V+ DS LVI+QL  EWETRDSKLI Y+KYI
Sbjct: 1457 AEYEACVMGLXAAIEKKIKVLDVFRDSALVIYQLRGEWETRDSKLIRYHKYI 1508

BLAST of Pay0021433 vs. NCBI nr
Match: XP_016899404.1 (PREDICTED: uncharacterized protein LOC107990505 [Cucumis melo] >KAA0054926.1 Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa] >TYK22715.1 Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa])

HSP 1 Score: 306.6 bits (784), Expect = 1.3e-79
Identity = 151/172 (87.79%), Postives = 156/172 (90.70%), Query Frame = 0

Query: 1   MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYEPMKFE 60
           MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYE MKFE
Sbjct: 1   MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYELMKFE 60

Query: 61  FLDEDVMTVSDTPQDTDIWTMFFDGATNKVGHGVGAILTFPNGKSYPLTAKLYFNCTNNM 120
           FLDEDVMTVSDTPQDT+IWTMFFDGATN+VGHGVGAILTFP+GKSYPLTAKLYF+CTNNM
Sbjct: 61  FLDEDVMTVSDTPQDTEIWTMFFDGATNEVGHGVGAILTFPDGKSYPLTAKLYFDCTNNM 120

Query: 121 ANMK------------HIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 161
           A  +             IKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI
Sbjct: 121 AEYETCSMGVKMAYDLKIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 172

BLAST of Pay0021433 vs. NCBI nr
Match: KAA0033845.1 (uncharacterized protein E6C27_scaffold43059G00190 [Cucumis melo var. makuwa] >TYK01227.1 uncharacterized protein E5676_scaffold49G00050 [Cucumis melo var. makuwa])

HSP 1 Score: 256.5 bits (654), Expect = 1.5e-64
Identity = 129/172 (75.00%), Postives = 138/172 (80.23%), Query Frame = 0

Query: 1   MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYEPMKFE 60
           MDPIKYIFEK SL GRIAKWQVL  EYDIIYVTR  +KGSAI DCLA+ PVEDYEPMK E
Sbjct: 64  MDPIKYIFEKSSLFGRIAKWQVLLLEYDIIYVTRKAMKGSAIVDCLANFPVEDYEPMKIE 123

Query: 61  FLDEDVMTVSDTPQDTDIWTMFFDGATNKVGHGVGAILTFPNGKSYPLTAKLYFNCTNNM 120
           F DEDVM VSDT QDT+IWTMFFDGATN+VGHGVGAILT  +GKSYPLT KLYF+CT+NM
Sbjct: 124 FPDEDVMIVSDTLQDTEIWTMFFDGATNEVGHGVGAILTSSDGKSYPLTTKLYFDCTHNM 183

Query: 121 ANMK------------HIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 161
           A  +             IKKLQVY DSLL IHQLN EWET DSKLIPY+KYI
Sbjct: 184 AEYEACSMGVQMAYDMKIKKLQVYRDSLLAIHQLNGEWETIDSKLIPYDKYI 235

BLAST of Pay0021433 vs. NCBI nr
Match: XP_008447313.1 (PREDICTED: uncharacterized protein LOC103489784 [Cucumis melo])

HSP 1 Score: 222.2 bits (565), Expect = 3.1e-54
Identity = 107/172 (62.21%), Postives = 126/172 (73.26%), Query Frame = 0

Query: 1   MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYEPMKFE 60
           MDPIKYIFEKPSLS +IAKW VL  E+DI+Y+TR  +KGS I DCL +LP+EDYEPMKF+
Sbjct: 64  MDPIKYIFEKPSLSEKIAKWHVLLLEFDIVYITRKAIKGSVIVDCLVELPIEDYEPMKFD 123

Query: 61  FLDEDVMTVSDTPQDTDIWTMFFDGATNKVGHGVGAILTFPNGKSYPLTAKLYFNCTNNM 120
           FLDED+MT+ +T  DT  WTM FD ATNKVGHG+G  L  P+ K YP T KLYF+CTNNM
Sbjct: 124 FLDEDIMTIVNTSSDT--WTMMFDRATNKVGHGMGVTLMSPDEKRYPFTTKLYFDCTNNM 183

Query: 121 ANMK------------HIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 161
              K             IK L+V  DSLLV+H+LN E+ETRD+K IPYN YI
Sbjct: 184 VEYKACSMGVRIAYEMKIKNLRVLGDSLLVVHKLNGEYETRDAKFIPYNDYI 233

BLAST of Pay0021433 vs. NCBI nr
Match: XP_022716542.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111275425 [Durio zibethinus])

HSP 1 Score: 214.2 bits (544), Expect = 8.5e-52
Identity = 105/172 (61.05%), Postives = 125/172 (72.67%), Query Frame = 0

Query: 1    MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYEPMKFE 60
            +DPIKYIFEKPSL+GRIA+WQV+ SEYDIIYVT+  +KGSAI + LAD  VEDYEPMK +
Sbjct: 1337 LDPIKYIFEKPSLTGRIARWQVMLSEYDIIYVTQKAIKGSAIAEFLADRAVEDYEPMKLD 1396

Query: 61   FLDEDVMTVSDTPQDTDIWTMFFDGATNKVGHGVGAILTFPNGKSYPLTAKLYFNCTNNM 120
            FLDEDVM + +       W M+FDGA N +GHG+GAIL  P+G  YP+TA+L FNCTNN+
Sbjct: 1397 FLDEDVMAIEENEPVEKSWRMYFDGAANALGHGIGAILISPDGHYYPITARLNFNCTNNV 1456

Query: 121  ANM------------KHIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 161
            A              K IK L V+ DS LVI+QL  EWETRDSKLI Y+KYI
Sbjct: 1457 AEYEACVMGLXAAIEKKIKVLDVFRDSALVIYQLRGEWETRDSKLIRYHKYI 1508

BLAST of Pay0021433 vs. NCBI nr
Match: XP_022751308.1 (uncharacterized protein LOC111300005, partial [Durio zibethinus])

HSP 1 Score: 210.7 bits (535), Expect = 9.4e-51
Identity = 104/172 (60.47%), Postives = 124/172 (72.09%), Query Frame = 0

Query: 1    MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYEPMKFE 60
            +DPIKYIFEKPSL+GRIA+WQV+ SEYDIIYVT+  +KGSAI + LAD  VEDYEPMK +
Sbjct: 1715 LDPIKYIFEKPSLTGRIARWQVMLSEYDIIYVTQKAIKGSAIAEFLADRAVEDYEPMKLD 1774

Query: 61   FLDEDVMTVSDTPQDTDIWTMFFDGATNKVGHGVGAILTFPNGKSYPLTAKLYFNCTNNM 120
            F DEDVM + +       W M+FDGA N +GHG+GAIL  P+G  YP+TA+L FNCTNN+
Sbjct: 1775 FPDEDVMAIEENKPVEKSWRMYFDGAANALGHGIGAILISPDGHYYPITARLNFNCTNNV 1834

Query: 121  ANM------------KHIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 161
            A              K IK L V+ DS LVI+QL  EWETRDSKLI Y+KYI
Sbjct: 1835 AEYEACVMGLQAAIEKKIKVLNVFGDSALVIYQLRGEWETRDSKLIRYHKYI 1886

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7UKV46.1e-8087.79Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. mak... [more]
A0A1S4DTT96.1e-8087.79uncharacterized protein LOC107990505 OS=Cucumis melo OX=3656 GN=LOC107990505 PE=... [more]
A0A5D3BPI87.2e-6575.00RNase H domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3BH421.5e-5462.21uncharacterized protein LOC103489784 OS=Cucumis melo OX=3656 GN=LOC103489784 PE=... [more]
A0A6P5WLA54.1e-5261.05Ribonuclease H OS=Durio zibethinus OX=66656 GN=LOC111275425 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_016899404.11.3e-7987.79PREDICTED: uncharacterized protein LOC107990505 [Cucumis melo] >KAA0054926.1 Ret... [more]
KAA0033845.11.5e-6475.00uncharacterized protein E6C27_scaffold43059G00190 [Cucumis melo var. makuwa] >TY... [more]
XP_008447313.13.1e-5462.21PREDICTED: uncharacterized protein LOC103489784 [Cucumis melo][more]
XP_022716542.18.5e-5261.05LOW QUALITY PROTEIN: uncharacterized protein LOC111275425 [Durio zibethinus][more]
XP_022751308.19.4e-5160.47uncharacterized protein LOC111300005, partial [Durio zibethinus][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 78..160
e-value: 6.9E-8
score: 34.6
NoneNo IPR availablePANTHERPTHR24559:SF322RNA-DIRECTED DNA POLYMERASE (REVERSE TRANSCRIPTASE), RIBONUCLEASE H-LIKE PROTEINcoord: 2..160
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 2..160
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 78..159

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0021433.1Pay0021433.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0016020 membrane
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity