Pay0021335 (gene) Melon (Payzawat) v1

BLAST of Pay0021335 vs. ExPASy Swiss-Prot
Match: Q0WQ75 (Vacuolar protein sorting-associated protein 51 homolog OS=Arabidopsis thaliana OX=3702 GN=VPS51 PE=1 SV=1)

HSP 1 Score: 982.2 bits (2538), Expect = 3.3e-285
Identity = 531/785 (67.64%), Postives = 635/785 (80.89%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60
           M  +  PMDEKAKRMRDLLSSFY+PD S+S S  GSS    +  + IN+TSF+ DQYM++
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSISTS--GSS--INASFDNINSTSFDADQYMDL 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           +++KSNLE LLQRHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRM +NI GME NM
Sbjct: 61  MIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           +QLL+KI+SVQS+SDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARL KCIK+EAY
Sbjct: 121 DQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240
            DAVRFYTGAMPI K YGD+SFQDC+RASEEAI +++KNLQ KLFSDSESIQ RAEAAVL
Sbjct: 181 GDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALV-NASSKDGNTSELVYGASHEAS 300
           LKQLD PVDSLK KLLEKLEQS   LQ+  E  ++ +  + SS D  +++      HE +
Sbjct: 241 LKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHEDA 300

Query: 301 VREFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTD 360
           VR F+EA+RAYR IF DS+ +L KLA+ L   HF+  E +IKK++ AAD L +F  +W D
Sbjct: 301 VRGFSEAIRAYREIFPDSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVWED 360

Query: 361 VLLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSL 420
           V+L+ EVL +A L D S +AAQV +KQ+V   FS L QDISD L +    +KE V+   L
Sbjct: 361 VVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQKEAVEGELL 420

Query: 421 QLELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFM 480
           ++ LEAS+KAVLQG+ +I  DFRQLL++++G+ I  +D I  W+Q+G QDFFR+L  +F+
Sbjct: 421 KVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEAQFL 480

Query: 481 LLSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           +LSGK    T S    E   ++K++AGL+LVLAQ+SVFIEQ  IPR+TEEIAASFSGG  
Sbjct: 481 VLSGK----TSSSNDIEGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTEEIAASFSGGNS 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           + +E GPAF+P E+CR+F AA EK L  YI+ R+Q++SVLL KRF+TPNWVKHKEPREVH
Sbjct: 541 QAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQILPEGT-RKHRRTDSNGS--TTSSRSNPLREEKLNRSNTQR 660
           M+VD+FL ELE VG EVKQ+LP+GT RKH+RTDSNGS  TTSSRSN L  +K+ RSN+QR
Sbjct: 601 MYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHNDKMARSNSQR 660

Query: 661 ARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQI 720
           ARSQL ETHLAKLFKQK+EIFT+VEFTQ SVVTT VKL LK+LQE+VRLQTFNRSGFQQI
Sbjct: 661 ARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQTFNRSGFQQI 720

Query: 721 QLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKD 780
           QLD+QFL+ PLKE  +DEAAIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAK+K+
Sbjct: 721 QLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLIQAKLAKSKE 777

Query: 781 QNPMT 782
            N  T
Sbjct: 781 HNNNT 777

BLAST of Pay0021335 vs. ExPASy Swiss-Prot
Match: Q54KG3 (Vacuolar protein sorting-associated protein 51 homolog OS=Dictyostelium discoideum OX=44689 GN=vps51 PE=3 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 5.1e-68
Identity = 222/799 (27.78%), Postives = 398/799 (49.81%), Query Frame = 0

Query: 11  KAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNILVQKSNLEGL 70
           ++KR+R+LL ++Y P     GS + S N    PL  I+  SFN + Y + +V+ S L  L
Sbjct: 147 ESKRVRNLLKTYYGPGIG-EGSDSSSMN---DPLN-IDGPSFNLNSYFDSIVKSSTLNQL 206

Query: 71  LQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSV 130
           +Q+  +M +EI+ LD D++ LVY+NY KFI+ATD IK+M  N+  ME  M  L + +  +
Sbjct: 207 IQKDNQMVSEIRTLDGDMKTLVYDNYTKFINATDIIKKMKTNVENMEEGMALLSKNMDLI 266

Query: 131 QSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGA 190
            + S+ +N++L  +R+ I++L   +   +K+QF+  LP+ L  C+  +AY  AVR+Y   
Sbjct: 267 TNCSEKINSTLSVRRDRIDQLSGLQKFFQKLQFLTALPSSLNHCLAMQAYNQAVRYYNSN 326

Query: 191 MPIFKAYGD-SSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVD 250
             I K Y    SFQ+ +   +  +  +   L E+L S S S     E+A +L  L  PV+
Sbjct: 327 SGILKQYSHIPSFQNIQNECDSIMKTMKDKLYERLSSLSTSQTDCVESAEVLMDLLEPVE 386

Query: 251 SLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASVREFTEAVRA 310
            ++ K LE  +  T+ L  N E  +   +    K+ N + L           E++  + +
Sbjct: 387 LVRSKYLESRKHHTITLLENLEKKSVEHITDYIKELNANFL----------SEYSYNITS 446

Query: 311 YRVIF------ADSDR-------QLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTI 370
           Y+ +F      +DS +       QL   ++DL  K+ +  +  +       + ++    I
Sbjct: 447 YKSLFINRLDGSDSKKERQQSLLQLEDFSKDLFNKYLNIAKSKLSSFKDPIEKIMALEII 506

Query: 371 WTDVLLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHT----RKKE 430
            +DV  LG  L+    +   + +    V   + F F  L + I + + Q+++    R+ E
Sbjct: 507 NSDVSRLGSELSSHEKITNIINS---TVHDQIDFYFDNLQKTIKEHIHQLNSTLNERRDE 566

Query: 431 GVQEYSLQLELEASKKAVLQGSMDILLDFRQL----LEDQSGLIINQRDSIVDWVQEGFQ 490
            ++ ++LQ   +A+ KA++    DI+L F  L    L  ++  + + +D+I   +Q   Q
Sbjct: 567 VLEGHNLQELSDATSKAIVN---DIILLFANLKPFFLPTETQFLSSYQDTIFTKIQVKLQ 626

Query: 491 DFFRALVDRFMLLSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITE 550
            FF  LV+   L       Y    A T  +  E+ +   +LVL+ I ++ E   I  + +
Sbjct: 627 QFFLFLVNIHFL------EYLDIIATT--SNREQFSGRFLLVLSSICLYFENKGITLVVQ 686

Query: 551 EIA--ASFSGGGIRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRT 610
            ++   + +  G +      +F   ++C+  R  G + L+++  + SQ++  +L K   +
Sbjct: 687 LMSEFITVAKQGAKKELNVFSFNAPDLCKRVRETGLQILNVFTRLSSQKLDKILKKGLES 746

Query: 611 ---PNWVKHKEPREVHMFVDLFLQELEAVGSEVKQILP------EGTRKHRRTDSNGSTT 670
               NW+  KEPR+V    D++L+E+    +E  ++LP         + H RT S G++ 
Sbjct: 747 IPNNNWLVLKEPRDVRSVNDIYLEEILKFQNETSKLLPVINHHHHSLKSHSRTGSGGNSV 806

Query: 671 SSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLK 730
           SS ++       +R N   + S         LF++K++    V+F   SV+  I+KLSLK
Sbjct: 807 SSNNSSSNTPDGHRRNPSASSSSSSNNTNTTLFEKKVD---HVDFNTLSVLIAIIKLSLK 866

Query: 731 TLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIP 777
           +  E +RL+TF  +G  QIQ+D+ +L+  L ++     + D LL E     +ERC+D +P
Sbjct: 867 SFNESLRLKTFGTNGCHQIQIDLHYLKLSLYDLFGSN-SFDNLLQECENTVTERCVDPLP 912

BLAST of Pay0021335 vs. ExPASy Swiss-Prot
Match: Q3UVL4 (Vacuolar protein sorting-associated protein 51 homolog OS=Mus musculus OX=10090 GN=Vps51 PE=1 SV=2)

HSP 1 Score: 231.5 bits (589), Expect = 3.3e-59
Identity = 212/787 (26.94%), Postives = 371/787 (47.14%), Query Frame = 0

Query: 10  EKAKRMRDLLSSFYS-PDASMSGSPTGSSNRYASPLEA--INTTSFNPDQYMNILVQKSN 69
           E+ ++   +L  +Y   +  ++G P G       PL+   +N   F+P+ Y++ L ++  
Sbjct: 28  ERRRKAHGMLKLYYGLSEGEVAGHPAG-----PDPLDPTDLNGAHFDPEVYLDKLRRECP 87

Query: 70  LEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEK 129
           L  L+    +M  +I+ LD+D+Q LVYENYNKFISATDTI++M N+   ME  M++L   
Sbjct: 88  LAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLATN 147

Query: 130 ILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRF 189
           +  + + S  ++ +L ++ E I KL     LLRK+QF+++LP+RL KC++  AY  AVR+
Sbjct: 148 MAVITNFSARISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRY 207

Query: 190 YTGAMPIFKAYGD-SSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVLLKQLD 249
              A  + + Y    SF+  +   +   A + + L+++          +AE   LL  L 
Sbjct: 208 QGRARAVLQQYQHLPSFRAIQDDCQVITARLAQQLRQRFREGCSGAPEQAECVELLLALG 267

Query: 250 FPVDSLKVKLLE----KLEQ--STLDLQLNAENLTSALVNASSKDGNTSELVYGASHEAS 309
            P + L  + L     +LE+  S+L+ +L        ++  + + GN             
Sbjct: 268 EPAEELCEEFLAHARGRLEEELSSLEAELGPSPPAPDVLEFTDRGGN-----------GF 327

Query: 310 VREFTEAVRAYRVIFADSD----RQLIKLAQDLVTKHFDSTEQFIKKQICAAD---LLLV 369
           V    +   AY+ +FA        +L   AQ+L  ++F   E+ + ++   +D   L+  
Sbjct: 328 VGGLCQVAAAYQELFAAQGPAGAEKLAAFAQELGGRYFALVERRLAQEQGGSDNSLLVRA 387

Query: 370 FGTIWTDVLLLGEVLNDAGLLDYSLKAAQVAVKQYVTF-------TFSRLLQDISDALTQ 429
                  +   G +L  AGL + + +  +   ++ ++         F   L D+  AL  
Sbjct: 388 LDRFHRRLRAPGALLAAAGLSESATEIVERVARERLSHHLQGLKAAFLSSLTDVRQALAA 447

Query: 430 VHTRKKEGVQEYSLQLELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQE 489
                KEG     L   + +S  + ++ S+  +  F       S     + +     V+E
Sbjct: 448 PRLAGKEGPSLAELLANVASSILSHIKTSLASVHLFTAKEVSFSNKPYFRGEFCSQGVRE 507

Query: 490 GFQDFFRALVDRFMLLSGKNNSYTQSQALTEATQAEKVNA---GLVLVLAQISVFIEQTA 549
           G             L+ G   S  Q+      +  EK  A    L+L+L+++ +  E   
Sbjct: 508 G-------------LIVGFIRSMCQTAQSFCDSPGEKGGATPPALLLLLSRLCLDYETAT 567

Query: 550 IPRITEEIAASFSGGGIRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTK 609
           I  I       F        +  P    + +C   R    + L  Y+ ++   IS +L K
Sbjct: 568 ISYILTLTDEQF-----LVQDQSPVTPVSTLCAEARETARRLLTHYVKVQGLVISQMLRK 627

Query: 610 RFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSN 669
              T +W+   EPR V   +   +++  A+  +V  +  EG RK + +DS+  T S  S+
Sbjct: 628 SVETRDWLSTLEPRNVRAVMKRVVEDTTAIDVQVGLLYEEGVRKAQSSDSSKRTFSVYSS 687

Query: 670 PLREEKLNRSNTQRARSQL-LETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQE 729
             ++ +   S T  A     L +++ KLF ++I++F+ VEF + SV+T I+K+SLKTL E
Sbjct: 688 SRQQGRYAPSYTPSAPMDTNLLSNIQKLFSERIDVFSPVEFNKVSVLTGIIKISLKTLLE 747

Query: 730 FVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPP 769
            VRL+TF R G QQ+Q+D  FL+  L     DE  +  LLDEV+ +A+ RC D +P+EP 
Sbjct: 748 CVRLRTFGRFGLQQVQVDCHFLQLYLWRFVADEELVHLLLDEVVASAALRCPDPVPMEPS 780

BLAST of Pay0021335 vs. ExPASy Swiss-Prot
Match: Q155U0 (Vacuolar protein sorting-associated protein 51 homolog OS=Danio rerio OX=7955 GN=vps51 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 3.6e-58
Identity = 224/834 (26.86%), Postives = 381/834 (45.68%), Query Frame = 0

Query: 13  KRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEA--INTTSFNPDQYMNILVQKSNLEGL 72
           +R+  +L  +Y  +        G +   A  L+   IN   F+P+ Y+N L ++ +L  L
Sbjct: 16  RRVHSMLKLYYGLNEE------GKATEQAESLDPCDINGPHFDPEIYLNKLRKECSLTEL 75

Query: 73  LQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSV 132
           +     M  +I++LD+D+Q LVYENYNKFISATDTI++M N+   ME  M+ L   + ++
Sbjct: 76  MDHESCMVKQIRSLDSDMQTLVYENYNKFISATDTIRKMKNDFKKMEDEMDCLSANMAAI 135

Query: 133 QSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGA 192
              S  ++ +L ++   I KL     LLRK+QF+++LPARL KC++ +AYA AV  +  A
Sbjct: 136 TEFSARISGTLQDQHAQITKLSGVHTLLRKLQFLFELPARLNKCLELQAYAQAVSSHRRA 195

Query: 193 MPIFKAYGD-SSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVD 252
             + + Y    SF+  +      +  + + L++K      S +  +E   LL QLD P +
Sbjct: 196 RCVLQQYSHMPSFRGIQDDCHVIMEQLAQQLRQKFRDGGSSAKDLSECVELLLQLDEPAE 255

Query: 253 SLKVKLLEKLE-QSTLDLQ-LNAENLTSALV--------------NASSKDGNTSELVYG 312
            L  K L   + +   DLQ L AE   SA+               N  S   + S     
Sbjct: 256 ELCDKFLSHAQSRLEADLQGLEAELKDSAVTDTGAGSVQKTSPGSNPVSPSSSVSNPFLS 315

Query: 313 ASHEASVREFTE------------AVRAYRVIFAD---------------SDRQLIKLAQ 372
            +    + EF +             + +Y+ +F +               ++ +L     
Sbjct: 316 PAAGTDILEFIDRGCNEFVSNLCLVIASYQELFINRPQESELASKNIPEMANGKLHVFVD 375

Query: 373 DLVTKHFDSTEQFIKKQICAADLLLVFGTI--------WTDVLLLGEVLNDAGLLDYSLK 432
            L  ++F   E+ I+++    D  L+   +            LL G  +   G  +  ++
Sbjct: 376 TLAARYFSLVERRIQEEKGVGDNSLLVRALDRFHRRLQAISKLLPGSAVPSQG-TEIVVR 435

Query: 433 AAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQLELEASKKAVLQG--SMD 492
           AA+  +KQY+    S L     D+LT V  R+       S+     +   A++ G  S D
Sbjct: 436 AARERIKQYL----SALQTFYHDSLTDV--RQALAAPRLSVGGASASGGGALVGGASSKD 495

Query: 493 ILLDFRQLLEDQSGLIINQRDSIV---------------------DWVQEGFQDFFRALV 552
                 +LL   S  I+NQ  S++                     ++  +G ++      
Sbjct: 496 APPSLPELLTSLSNFILNQLKSVLASVHLFTAKDITFSNKPYFKGEFCSQGVREGLVVSF 555

Query: 553 DRFMLLSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFS 612
            +F+  S +   Y +S       +       L+L+L+++ +  E + I  I       F 
Sbjct: 556 IKFICQSSR--QYCES----AGDRGGSTPPALLLLLSRLCLDYETSTISYILTLTDEQF- 615

Query: 613 GGGIRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEP 672
                   + P      +C   R A +K L+ Y+ ++   IS +L K   T +WV   EP
Sbjct: 616 ----LVQHHTPVTPVTALCAEAREAAQKLLNHYVKVQGLIISQMLRKSVETRDWVNTIEP 675

Query: 673 REVHMFVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQ 732
           R V   +   +++  ++  +V  +  EG RK   +DS+  T S  S+  ++ +   S T 
Sbjct: 676 RNVRAVMKRVVEDTTSIDVQVGLLYEEGVRKAHSSDSSKRTFSVYSSSRQQIRYAPSYTP 735

Query: 733 RARSQL-LETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQ 769
            A     L +++ KLF ++I+IF+ VEF + SV+T I+K+SLKT  E VRL+TF R G Q
Sbjct: 736 SAPMDTNLLSNIHKLFSERIDIFSPVEFNKVSVLTGIIKISLKTFLECVRLRTFGRYGLQ 795

BLAST of Pay0021335 vs. ExPASy Swiss-Prot
Match: Q9UID3 (Vacuolar protein sorting-associated protein 51 homolog OS=Homo sapiens OX=9606 GN=VPS51 PE=1 SV=2)

HSP 1 Score: 228.0 bits (580), Expect = 3.6e-58
Identity = 213/781 (27.27%), Postives = 363/781 (46.48%), Query Frame = 0

Query: 10  EKAKRMRDLLSSFYS-PDASMSGSPTGSSNRYASPLEA--INTTSFNPDQYMNILVQKSN 69
           E+ ++   +L  +Y   +   +G P G       PL+   +N   F+P+ Y++ L ++  
Sbjct: 28  ERRRKAHGMLKLYYGLSEGEAAGRPAG-----PDPLDPTDLNGAHFDPEVYLDKLRRECP 87

Query: 70  LEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEK 129
           L  L+    +M  +I+ LD+D+Q LVYENYNKFISATDTI++M N+   ME  M++L   
Sbjct: 88  LAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLATN 147

Query: 130 ILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRF 189
           +  +   S  ++ +L ++ E I KL     LLRK+QF+++LP+RL KC++  AY  AVR+
Sbjct: 148 MAVITDFSARISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRY 207

Query: 190 YTGAMPIFKAYGD-SSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVLLKQLD 249
              A  + + Y    SF+  +   +   A + + L+++          +AE   LL  L 
Sbjct: 208 QGRAQAVLQQYQHLPSFRAIQDDCQVITARLAQQLRQRFREGGSGAPEQAECVELLLALG 267

Query: 250 FPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASVREFTE 309
            P + L     E L  +   L+    NL + L  +           +G S    V    +
Sbjct: 268 EPAEEL---CEEFLAHARGRLEKELRNLEAELGPSPPAPDVLEFTDHGGS--GFVGGLCQ 327

Query: 310 AVRAYRVIFADSD----RQLIKLAQDLVTKHFDSTEQFIKKQICAAD---LLLVFGTIWT 369
              AY+ +FA        +L   A+ L +++F   E+ + ++    D   L+        
Sbjct: 328 VAAAYQELFAAQGPAGAEKLAAFARQLGSRYFALVERRLAQEQGGGDNSLLVRALDRFHR 387

Query: 370 DVLLLGEVLNDAGLLDYSLKAAQVAVK-------QYVTFTFSRLLQDISDALTQVHTRKK 429
            +   G +L  AGL D + +  +   +       Q +   F   L D+  AL       K
Sbjct: 388 RLRAPGALLAAAGLADAATEIVERVARERLGHHLQGLRAAFLGCLTDVRQALAAPRVAGK 447

Query: 430 EGVQEYSLQLELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFF 489
           EG     L   + +S  + ++ S+  +  F       S     + +     V+EG     
Sbjct: 448 EGPGLAELLANVASSILSHIKASLAAVHLFTAKEVSFSNKPYFRGEFCSQGVREG----- 507

Query: 490 RALVDRFMLLSGKNNSYTQSQALTEATQAEKVNA---GLVLVLAQISVFIEQTAIPRITE 549
                   L+ G  +S  Q+      +  EK  A    L+L+L+++ +  E   I  I  
Sbjct: 508 --------LIVGFVHSMCQTAQSFCDSPGEKGGATPPALLLLLSRLCLDYETATISYILT 567

Query: 550 EIAASFSGGGIRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPN 609
                F        +  P    + +C   R    + L  Y+ ++   IS +L K   T +
Sbjct: 568 LTDEQF-----LVQDQFPVTPVSTLCAEARETARRLLTHYVKVQGLVISQMLRKSVETRD 627

Query: 610 WVKHKEPREVHMFVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEK 669
           W+   EPR V   +   +++  A+  +V  +  EG RK + +DS+  T S  S+  ++ +
Sbjct: 628 WLSTLEPRNVRAVMKRVVEDTTAIDVQVGLLYEEGVRKAQSSDSSKRTFSVYSSSRQQGR 687

Query: 670 LNRSNTQRARSQL-LETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQT 729
              S T  A     L +++ KLF ++I++F+ VEF + SV+T I+K+SLKTL E VRL+T
Sbjct: 688 YAPSYTPSAPMDTNLLSNIQKLFSERIDVFSPVEFNKVSVLTGIIKISLKTLLECVRLRT 747

Query: 730 FNRSGFQQIQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLI 769
           F R G QQ+Q+D  FL+  L     DE  +  LLDEV+ +A+ RC D +P+EP +++ + 
Sbjct: 748 FGRFGLQQVQVDCHFLQLYLWRFVADEELVHLLLDEVVASAALRCPDPVPMEPSVVEVIC 780

BLAST of Pay0021335 vs. ExPASy TrEMBL
Match: A0A1S3CES5 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucumis melo OX=3656 GN=LOC103500085 PE=3 SV=1)

HSP 1 Score: 1474.9 bits (3817), Expect = 0.0e+00
Identity = 781/782 (99.87%), Postives = 781/782 (99.87%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60
           MEIDDVPMDEKAKRMRDLLSSFYSPDASMS SPTGSSNRYASPLEAINTTSFNPDQYMNI
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSSSPTGSSNRYASPLEAINTTSFNPDQYMNI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300
           LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300

Query: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360
           REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV
Sbjct: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360

Query: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ
Sbjct: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420

Query: 421 LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480
           LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML
Sbjct: 421 LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR
Sbjct: 481 LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540

Query: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600
           GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660
           FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660

Query: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM
Sbjct: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720

Query: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780
           QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780

Query: 781 TP 783
           TP
Sbjct: 781 TP 782

BLAST of Pay0021335 vs. ExPASy TrEMBL
Match: A0A6J1EMM4 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita moschata OX=3662 GN=LOC111435972 PE=3 SV=1)

HSP 1 Score: 1387.1 bits (3589), Expect = 0.0e+00
Identity = 736/783 (94.00%), Postives = 755/783 (96.42%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60
           MEID+ PMDEK KRMRDLLSSFYSPDAS SGS  GSSNRYASPLEAINTTSFNPDQYM+I
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQ+HVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLL KVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLCKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFK YGDSSFQDCKRASEEAIA+VLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQLNAE+L+SALV  SSKDGN SE VYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASV 300

Query: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360
           REF EA+RAYRVIFADSDRQLIKLAQDLVTKHFD+TEQFI+KQICAADLL VFG IWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKK-EGVQEYSL 420
           LLLGEVLNDAGL DYSLKAAQVAVKQYVT TFSRLLQDISDAL QVHTRKK EGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFM 480
           QL LEASKKAVLQGSMD+LLDFRQLLEDQSGL I+QRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           LLSGKN+S++QSQ L EATQAEKV AGLVLVLAQ+SVFIEQTAIPRITEEIAASFSGGG 
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEAVGSEVKQILP+GTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTT VKLSLKTL EFVRLQTFNRSGFQQ+QLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNP 780
           MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLD+IPLEPPILDKLIQAKLAKAKDQNP
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDTIPLEPPILDKLIQAKLAKAKDQNP 780

Query: 781 MTP 783
            +P
Sbjct: 781 TSP 783

BLAST of Pay0021335 vs. ExPASy TrEMBL
Match: A0A6J1JAN2 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita maxima OX=3661 GN=LOC111483261 PE=3 SV=1)

HSP 1 Score: 1382.1 bits (3576), Expect = 0.0e+00
Identity = 733/783 (93.61%), Postives = 753/783 (96.17%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60
           MEID+  MDEK KRMRDLLSSFYSPDAS SGS  GSSNRYASPLEAINTTSFNPDQYM+I
Sbjct: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQ+HVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFK YGDSSFQDCKRASEEAIA+VLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQLNAE+L+SALV  SSKDGN SE VYGASHE SV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEGSV 300

Query: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360
           REF EA+RAYRVIFADSDRQLIKLAQDLVTKHF++ EQFIKKQICAADLL VFG IWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFNAIEQFIKKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKK-EGVQEYSL 420
           LLLGEVLNDAGL DYSLKAAQVAVKQYVT  FSRLLQDISDAL QVHTRKK EGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCMFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFM 480
           QL LEASKKAVLQGSMD+LLDFRQLLEDQSGL I+QRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           LLSGKN+SY+QSQ L EATQAEKV AGLVLVLAQISVFIEQTAIPRITEEIAASFSGGG 
Sbjct: 481 LLSGKNSSYSQSQVLPEATQAEKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEA+GSEVKQILP+GTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTT VKLSLKTL EF+RLQTFNRSGFQQ+QLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFIRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNP 780
           MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLD+IPLEPPILDKLI+AKLAKAKDQNP
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIKAKLAKAKDQNP 780

Query: 781 MTP 783
           M+P
Sbjct: 781 MSP 783

BLAST of Pay0021335 vs. ExPASy TrEMBL
Match: A0A6J1K9H7 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita maxima OX=3661 GN=LOC111492889 PE=3 SV=1)

HSP 1 Score: 1358.2 bits (3514), Expect = 0.0e+00
Identity = 721/782 (92.20%), Postives = 748/782 (95.65%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60
           MEI+DVPMDEKAKRMRDLLSSFYSPDASMSGS TGSSNRYASPLE INTTSFNPDQYM+I
Sbjct: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTGSSNRYASPLETINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQK+NLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKANLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIA+VLK LQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKKLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQLNA++L+S L+ AS K+ N SE  YGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNADDLSSGLLIASPKERNDSESAYGASHEASV 300

Query: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360
           REF EAVRAYRVIFADSDRQLIKLAQDLVTKHFD+ EQFIKKQI AADLL VFG IWTDV
Sbjct: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDAAEQFIKKQIFAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDA L DYSLKAAQVAVKQYVT TFSRLLQ+ISDALT+VHTRKKE VQEYSLQ
Sbjct: 361 LLLGEVLNDACLHDYSLKAAQVAVKQYVTCTFSRLLQNISDALTRVHTRKKEDVQEYSLQ 420

Query: 421 LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480
           L LEA KKAVLQGSMD+LLDFR LLE+QSGLIINQRDSIVDWVQEGFQDFFR LVDRFML
Sbjct: 421 LALEAGKKAVLQGSMDVLLDFRLLLENQSGLIINQRDSIVDWVQEGFQDFFRELVDRFML 480

Query: 481 LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSGKNNSY+QSQ LTEA QA+KV AGLVLVLAQIS+FIEQTAI RITEEIAASFSGGG+R
Sbjct: 481 LSGKNNSYSQSQVLTEANQADKVVAGLVLVLAQISIFIEQTAIHRITEEIAASFSGGGVR 540

Query: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600
           GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660
           FVDLFLQELEAVGSEV+QIL +GTRKHRRT+SNGSTTSSRS PLREEKLNRS+ QRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVRQILLQGTRKHRRTNSNGSTTSSRSGPLREEKLNRSSAQRARSQ 660

Query: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLE+HLAKLFKQKIEIFTRVEFTQGSVVTT VKLSLKTLQEFVRLQT+NRSGFQQIQLDM
Sbjct: 661 LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTYNRSGFQQIQLDM 720

Query: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780
           QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA+DQN +
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNSI 780

Query: 781 TP 783
           +P
Sbjct: 781 SP 782

BLAST of Pay0021335 vs. ExPASy TrEMBL
Match: A0A6J1GBC0 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita moschata OX=3662 GN=LOC111452610 PE=3 SV=1)

HSP 1 Score: 1358.2 bits (3514), Expect = 0.0e+00
Identity = 720/782 (92.07%), Postives = 749/782 (95.78%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60
           MEI+DVPMDEKAKRMRDLLSSFYSPDASMSGS TGSSNRYASPLE INTTSFNPDQYM+I
Sbjct: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTGSSNRYASPLETINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQK+NLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKANLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIA+VLK LQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKKLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQLNA++L+S L+ AS K+ N SE  YGA+HEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNADDLSSGLLIASPKERNDSESAYGAAHEASV 300

Query: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360
           REF EAVRAYRVIFADSD QLIKLAQDLVTKHFD+ EQFIKKQI AADLL VFG IWTDV
Sbjct: 301 REFAEAVRAYRVIFADSDMQLIKLAQDLVTKHFDAAEQFIKKQIFAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDA L DYSLKAAQVAVKQYVT TFSRLLQ+ISDALT+VHTRKKE VQEYSLQ
Sbjct: 361 LLLGEVLNDACLHDYSLKAAQVAVKQYVTCTFSRLLQNISDALTRVHTRKKEDVQEYSLQ 420

Query: 421 LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480
           L LEA KKAVLQGSMD+LLDFR LLE+QSGLIINQRDSIVDWVQEGFQDFFRALVDRFML
Sbjct: 421 LALEAGKKAVLQGSMDVLLDFRLLLENQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSGKNNSY+QSQ LTEATQA+KV AGLVLVLAQIS+FIEQTAI RITEEIAASFSGGG+R
Sbjct: 481 LSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISIFIEQTAIHRITEEIAASFSGGGVR 540

Query: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600
           GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660
           FVDLFLQELEAVGSEV+QIL +GTRKHRRT+SNGSTTSSRS PLREEKLN++N QRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVRQILLQGTRKHRRTNSNGSTTSSRSGPLREEKLNKTNAQRARSQ 660

Query: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLE+HLAKLFKQKIEIFTRVEFTQGSVVTT VKLSLKTLQEFVRLQT+NRSGFQQIQLDM
Sbjct: 661 LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTYNRSGFQQIQLDM 720

Query: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780
           QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA+DQN +
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNSI 780

Query: 781 TP 783
           +P
Sbjct: 781 SP 782

BLAST of Pay0021335 vs. NCBI nr
Match: XP_008461504.1 (PREDICTED: vacuolar protein sorting-associated protein 51 homolog [Cucumis melo] >XP_008461505.1 PREDICTED: vacuolar protein sorting-associated protein 51 homolog [Cucumis melo])

HSP 1 Score: 1474.9 bits (3817), Expect = 0.0e+00
Identity = 781/782 (99.87%), Postives = 781/782 (99.87%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60
           MEIDDVPMDEKAKRMRDLLSSFYSPDASMS SPTGSSNRYASPLEAINTTSFNPDQYMNI
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSSSPTGSSNRYASPLEAINTTSFNPDQYMNI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300
           LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300

Query: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360
           REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV
Sbjct: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360

Query: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ
Sbjct: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420

Query: 421 LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480
           LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML
Sbjct: 421 LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR
Sbjct: 481 LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540

Query: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600
           GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660
           FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660

Query: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM
Sbjct: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720

Query: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780
           QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780

Query: 781 TP 783
           TP
Sbjct: 781 TP 782

BLAST of Pay0021335 vs. NCBI nr
Match: XP_004139639.1 (vacuolar protein sorting-associated protein 51 homolog [Cucumis sativus] >XP_011659140.1 vacuolar protein sorting-associated protein 51 homolog [Cucumis sativus] >KGN44805.2 hypothetical protein Csa_015985 [Cucumis sativus])

HSP 1 Score: 1461.4 bits (3782), Expect = 0.0e+00
Identity = 773/782 (98.85%), Postives = 779/782 (99.62%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60
           MEI+DVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI
Sbjct: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300
           LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLT+ALVNASSKDGN+SELVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTTALVNASSKDGNSSELVYGASHEASV 300

Query: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360
           REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFG IWTDV
Sbjct: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420
           LL GEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ
Sbjct: 361 LLFGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420

Query: 421 LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480
           LELEASKKAVLQGSMD+LL+FRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML
Sbjct: 421 LELEASKKAVLQGSMDVLLEFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSGKNNSYTQSQALTEATQAEKV AGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR
Sbjct: 481 LSGKNNSYTQSQALTEATQAEKVIAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540

Query: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600
           GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVK+KEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKYKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660
           FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660

Query: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM
Sbjct: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720

Query: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780
           QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780

Query: 781 TP 783
           TP
Sbjct: 781 TP 782

BLAST of Pay0021335 vs. NCBI nr
Match: XP_038887792.1 (vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887793.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887794.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887795.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887796.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 1412.1 bits (3654), Expect = 0.0e+00
Identity = 746/782 (95.40%), Postives = 762/782 (97.44%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60
           MEIDDVPMDEKAKRMRDLLSSFY+PDASMS S  GSSNRY SPLEAINTTSFNPDQYM+I
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYAPDASMSSSSMGSSNRYTSPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSFQDCKRASEE IAVVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEVIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQ+NAE+L+SALVN SSKDG+TSELVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQVNAEDLSSALVNGSSKDGSTSELVYGASHEASV 300

Query: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360
           REF EAVRAYRVIFADSDRQLIKLAQDLVTKHFD+TEQFIKKQICAADLLLVFG IWTDV
Sbjct: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICAADLLLVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDAGL DYSLKAAQVAVKQYV   FSRLLQDISDALTQVHTRKKEG QEYSLQ
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVKCKFSRLLQDISDALTQVHTRKKEGAQEYSLQ 420

Query: 421 LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480
           L LEA+KKAVLQGSMD+LLDFRQLLED+SGLIINQRDSIVDWVQEGFQDFFRALVDRFML
Sbjct: 421 LALEATKKAVLQGSMDVLLDFRQLLEDKSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSGKNNSY+QSQ LTEATQAEKV AGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR
Sbjct: 481 LSGKNNSYSQSQVLTEATQAEKVIAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540

Query: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600
           GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRIS+L TKRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISILSTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660
           FVDLFLQELEAVGSEVKQILP+GTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660

Query: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLETHLAKLFKQKIEIFTRVEFTQGSVVTT+VKLSLKTLQEFVR QTFNRSGFQQIQLDM
Sbjct: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTLVKLSLKTLQEFVRFQTFNRSGFQQIQLDM 720

Query: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780
           QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQN +
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNSI 780

Query: 781 TP 783
           +P
Sbjct: 781 SP 782

BLAST of Pay0021335 vs. NCBI nr
Match: KAG6577400.1 (Vacuolar protein sorting-associated protein 51-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1387.5 bits (3590), Expect = 0.0e+00
Identity = 735/783 (93.87%), Postives = 755/783 (96.42%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60
           MEID+ PMDEK KRMRDLLSSFYSPDAS SGS  GSSNRYASPLEAINTTSFNPDQYM+I
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQ+HVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFK YGDSSFQDCKRASEEAIA+VLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQLNAE+L+SALV  SSKDGN SE VYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASV 300

Query: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360
           REF EA+RAYRVIFADSDRQLIKLAQDLVTKHFD+ EQFI+KQICAADLL VFG IWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKK-EGVQEYSL 420
           LLLGEVLNDAGL DYSLKAAQVAVKQYVT TFSRLLQDISDAL QVHTRKK EGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFM 480
           QL LEASKKAVLQGSMD+LLDFRQLLEDQSGL I+QRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           LLSGKN+S++QSQ L EATQAEKV AGLVLVLAQ+SVFIEQTAIPRITEEIAASFSGGG 
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEA+GSEVKQILP+GTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTT VKLSLKTL EFVRLQTFNRSGFQQ+QLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNP 780
           MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLD+IPLEPPILDKLIQAKLAKAKDQNP
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNP 780

Query: 781 MTP 783
            +P
Sbjct: 781 TSP 783

BLAST of Pay0021335 vs. NCBI nr
Match: XP_022929381.1 (vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929382.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929383.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929384.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 1387.1 bits (3589), Expect = 0.0e+00
Identity = 736/783 (94.00%), Postives = 755/783 (96.42%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60
           MEID+ PMDEK KRMRDLLSSFYSPDAS SGS  GSSNRYASPLEAINTTSFNPDQYM+I
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNLEGLLQ+HVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLL KVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLCKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFK YGDSSFQDCKRASEEAIA+VLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQLNAE+L+SALV  SSKDGN SE VYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASV 300

Query: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360
           REF EA+RAYRVIFADSDRQLIKLAQDLVTKHFD+TEQFI+KQICAADLL VFG IWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKK-EGVQEYSL 420
           LLLGEVLNDAGL DYSLKAAQVAVKQYVT TFSRLLQDISDAL QVHTRKK EGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFM 480
           QL LEASKKAVLQGSMD+LLDFRQLLEDQSGL I+QRDSIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           LLSGKN+S++QSQ L EATQAEKV AGLVLVLAQ+SVFIEQTAIPRITEEIAASFSGGG 
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEAVGSEVKQILP+GTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTT VKLSLKTL EFVRLQTFNRSGFQQ+QLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNP 780
           MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLD+IPLEPPILDKLIQAKLAKAKDQNP
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDTIPLEPPILDKLIQAKLAKAKDQNP 780

Query: 781 MTP 783
            +P
Sbjct: 781 TSP 783

BLAST of Pay0021335 vs. TAIR 10
Match: AT4G02030.1 (Vps51/Vps67 family (components of vesicular transport) protein )

HSP 1 Score: 982.2 bits (2538), Expect = 2.3e-286
Identity = 531/785 (67.64%), Postives = 635/785 (80.89%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60
           M  +  PMDEKAKRMRDLLSSFY+PD S+S S  GSS    +  + IN+TSF+ DQYM++
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSISTS--GSS--INASFDNINSTSFDADQYMDL 60

Query: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           +++KSNLE LLQRHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRM +NI GME NM
Sbjct: 61  MIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           +QLL+KI+SVQS+SDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARL KCIK+EAY
Sbjct: 121 DQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240
            DAVRFYTGAMPI K YGD+SFQDC+RASEEAI +++KNLQ KLFSDSESIQ RAEAAVL
Sbjct: 181 GDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVL 240

Query: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALV-NASSKDGNTSELVYGASHEAS 300
           LKQLD PVDSLK KLLEKLEQS   LQ+  E  ++ +  + SS D  +++      HE +
Sbjct: 241 LKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHEDA 300

Query: 301 VREFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTD 360
           VR F+EA+RAYR IF DS+ +L KLA+ L   HF+  E +IKK++ AAD L +F  +W D
Sbjct: 301 VRGFSEAIRAYREIFPDSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVWED 360

Query: 361 VLLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSL 420
           V+L+ EVL +A L D S +AAQV +KQ+V   FS L QDISD L +    +KE V+   L
Sbjct: 361 VVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQKEAVEGELL 420

Query: 421 QLELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFM 480
           ++ LEAS+KAVLQG+ +I  DFRQLL++++G+ I  +D I  W+Q+G QDFFR+L  +F+
Sbjct: 421 KVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEAQFL 480

Query: 481 LLSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           +LSGK    T S    E   ++K++AGL+LVLAQ+SVFIEQ  IPR+TEEIAASFSGG  
Sbjct: 481 VLSGK----TSSSNDIEGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTEEIAASFSGGNS 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           + +E GPAF+P E+CR+F AA EK L  YI+ R+Q++SVLL KRF+TPNWVKHKEPREVH
Sbjct: 541 QAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQILPEGT-RKHRRTDSNGS--TTSSRSNPLREEKLNRSNTQR 660
           M+VD+FL ELE VG EVKQ+LP+GT RKH+RTDSNGS  TTSSRSN L  +K+ RSN+QR
Sbjct: 601 MYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHNDKMARSNSQR 660

Query: 661 ARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQI 720
           ARSQL ETHLAKLFKQK+EIFT+VEFTQ SVVTT VKL LK+LQE+VRLQTFNRSGFQQI
Sbjct: 661 ARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQTFNRSGFQQI 720

Query: 721 QLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKD 780
           QLD+QFL+ PLKE  +DEAAIDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAK+K+
Sbjct: 721 QLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLIQAKLAKSKE 777

Query: 781 QNPMT 782
            N  T
Sbjct: 781 HNNNT 777

BLAST of Pay0021335 vs. TAIR 10
Match: AT4G02030.2 (Vps51/Vps67 family (components of vesicular transport) protein )

HSP 1 Score: 968.4 bits (2502), Expect = 3.5e-282
Identity = 531/810 (65.56%), Postives = 635/810 (78.40%), Query Frame = 0

Query: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60
           M  +  PMDEKAKRMRDLLSSFY+PD S+S S  GSS    +  + IN+TSF+ DQYM++
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSISTS--GSS--INASFDNINSTSFDADQYMDL 60

Query: 61  L-------------------------VQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYEN 120
           +                         ++KSNLE LLQRHV+MAAEIKNLDTDLQMLVYEN
Sbjct: 61  MIRLFDLGLSDLPAMVDSSELNWVYEIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYEN 120

Query: 121 YNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTR 180
           YNKFISATDTIKRM +NI GME NM+QLL+KI+SVQS+SDGVNTSLFEKREHIEKLHRTR
Sbjct: 121 YNKFISATDTIKRMKSNIFGMEGNMDQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTR 180

Query: 181 NLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAV 240
           NLLRKVQFIYDLPARL KCIK+EAY DAVRFYTGAMPI K YGD+SFQDC+RASEEAI +
Sbjct: 181 NLLRKVQFIYDLPARLQKCIKSEAYGDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEI 240

Query: 241 VLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTS 300
           ++KNLQ KLFSDSESIQ RAEAAVLLKQLD PVDSLK KLLEKLEQS   LQ+  E  ++
Sbjct: 241 IIKNLQTKLFSDSESIQARAEAAVLLKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEAST 300

Query: 301 ALV-NASSKDGNTSELVYGASHEASVREFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFD 360
            +  + SS D  +++      HE +VR F+EA+RAYR IF DS+ +L KLA+ L   HF+
Sbjct: 301 LVEDDDSSNDTESNDQHPAKIHEDAVRGFSEAIRAYREIFPDSEERLFKLARALTAMHFE 360

Query: 361 STEQFIKKQICAADLLLVFGTIWTDVLLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSR 420
             E +IKK++ AAD L +F  +W DV+L+ EVL +A L D S +AAQV +KQ+V   FS 
Sbjct: 361 YMELYIKKRVSAADFLGIFRIVWEDVVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSH 420

Query: 421 LLQDISDALTQVHTRKKEGVQEYSLQLELEASKKAVLQGSMDILLDFRQLLEDQSGLIIN 480
           L QDISD L +    +KE V+   L++ LEAS+KAVLQG+ +I  DFRQLL++++G+ I 
Sbjct: 421 LQQDISDTLLKFDINQKEAVEGELLKVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIK 480

Query: 481 QRDSIVDWVQEGFQDFFRALVDRFMLLSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQI 540
            +D I  W+Q+G QDFFR+L  +F++LSGK    T S    E   ++K++AGL+LVLAQ+
Sbjct: 481 MKDLISGWIQKGSQDFFRSLEAQFLVLSGK----TSSSNDIEGKSSDKIHAGLILVLAQL 540

Query: 541 SVFIEQTAIPRITEEIAASFSGGGIRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQ 600
           SVFIEQ  IPR+TEEIAASFSGG  + +E GPAF+P E+CR+F AA EK L  YI+ R+Q
Sbjct: 541 SVFIEQKVIPRVTEEIAASFSGGNSQAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQ 600

Query: 601 RISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEVKQILPEGT-RKHRRTDSN 660
           ++SVLL KRF+TPNWVKHKEPREVHM+VD+FL ELE VG EVKQ+LP+GT RKH+RTDSN
Sbjct: 601 KVSVLLRKRFKTPNWVKHKEPREVHMYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSN 660

Query: 661 GS--TTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTI 720
           GS  TTSSRSN L  +K+ RSN+QRARSQL ETHLAKLFKQK+EIFT+VEFTQ SVVTT 
Sbjct: 661 GSNTTTSSRSNTLHNDKMARSNSQRARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTT 720

Query: 721 VKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRTPLKEIADDEAAIDFLLDEVIVAASER 780
           VKL LK+LQE+VRLQTFNRSGFQQIQLD+QFL+ PLKE  +DEAAIDFLLDEVIVAASER
Sbjct: 721 VKLCLKSLQEYVRLQTFNRSGFQQIQLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASER 780

Query: 781 CLDSIPLEPPILDKLIQAKLAKAKDQNPMT 782
           CLD IPLEPPILDKLIQAKLAK+K+ N  T
Sbjct: 781 CLDVIPLEPPILDKLIQAKLAKSKEHNNNT 802