Pay0021069 (gene) Melon (Payzawat) v1

Overview
NamePay0021069
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationchr05: 1857440 .. 1860484 (-)
RNA-Seq ExpressionPay0021069
SyntenyPay0021069
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAATATCTATAGGGAAATACATCAAGTTACAGGGTAAGAAATGGTCCACATTCCAGCTTTCGAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTATTGCTCTTTGGTTCTTCTCTCCTCCTGAAACTTCTCACCACCGTCTCTCGTCCGTCCGGCATACAGCATTTTTAAGGTAATCCGGTAAAGCAATGGGTGTGATTCTTTAGGCTTGTTTGTTACATGAATCAACTGATTTTGATTTCTATTTTGGGAATATTGTTGTTGTTGTAGTGATGGGTTGGGGAAGAGAGGGGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTTTATCATAATTTCTTAGTAAGTTTCTAGAATTGTGGTTTTTTTTGGAAAAAGAAAGGAAAATTTGTTTTGTTAAAATGGTTGGCTGATATTGGAATGGTGTTGGACAGTCCAAGGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCTCACATGGAGAAATCAACTGTGGTTGATAGCGAAACTGGCAAGAGTGTGGATAGCAGGTATATATTTGTTATTTTCTTTGCTGTCCTGTTACTAACAGAAACAGAACTTGTCAATGTCAATTATTGACTATGAACTAACTCTGGTGGGGCTTACACTTTGACACATTTAAAGTGTAAGAAAAAGTAATCTCAAAGAGAGTAAATTTGAACTAAACTCATATTTTAAAGATGGTTTATCTATATAGTCGTTTCTCATTTAGAACTTAAAAGCCAAAGGAACTGAGAGACTAATCCCAAACGTGGCTGCGTCTAATGGTGCAAGCAAAAAGGATAAAAAAAAATAGTGTAATATACTATTTTACTGTAACCTACTAGCTTAACCTTTTAAGTTGATTTTTAATTTAATACAGTATTAAGTAGGAGGTCTCCGATCTTTGTAGTGTCATTTGTTCCCGCCACAAAATTGAAAAGCTAACCCTTTCTCCTGGACTAAGCTTTTGGGTCGATCAGTAATTTAACTATTTGAATTATACTGTGTAGGAGCGAAGATAGTATGAATACACTTTTGTTGTTCCCTTTGGTTTGATTGGTTTAAGTGAGATGGGTAGATTGGTCGGTTCGGTTCTTGAAAAATCAGTTCGGTAAAAGTCCAACCTGAACTGATCACAATAGAATGGTTATGTATGAAGCATAATAGAAGGGTATAAAGTAGAACAATACAATTTCAATGAACAAGGCAAACTTACAACTTTTTGCTGTACAATGTCACGTCCACAGTGTGCGAACCAGTTCTGGGATGTTTCTGAATAGAGGGCAGGACAAAATCATCAGTAACATAGAGAAAAGAATAGCAGATTTTACATTCATTCCTATAGGTAAATAATTCAAAACTTTCATATCACCATGGTTAATTTCTGAAGCATTTTGTGATAGTTCCTATATGTAAGGGCTCATTTAGGAATACTTTTCAAAACAGTTTTTGAAAAACATGTTCGAATCACTGTTTTTTGCTAGTTGTTTTATTTCGTCTGTGTTAGTGATTTCATTCAATATATCAATCTTTACAATGCATTCAACTGTAGTTATATTGAATAGTTCTTCAAAATAGAGTTGCAACCTGAACATAATTCTCAAACTAACCTTTGTTAGACTAACCAAACATTGGCGAAATATTCGTTAAATTTCATGTGGGATTTTGTTCACCATTCTTGTCTTTTGGCCCTAACCAAAAAGGGCTTTGGTGAATGAGCTATTCATTTGCTGCTTGTTGTTTTGTTGATGTCACTGGCATTTTTGGTCTTAATACGTTTTGGTACTGATGTTGATATTCGCAATTTGAAACATGCATACAGAGTGTATCGTGAAGTTGAAATGATAGACAATTGTTATAATTTAGTTAATCTGGGTTTAGTTGTAAATTTTGATTGGTTAGTGTTTGATTATTAGTTTATTTGTTATCCCCTTTGAGGTTTGTATTGATTTCCCTTTATTTATTTACTTTTATTTTTAGATCTTTGTTTTATAGACTAGAGAGTTTTCTCTCAACTCCAAGGACAAATTTCTCTTATTTGGCCATGGATTTGGTCTATTCTCAAATCTGCTTCATTAGCATGGAGAATGTGGGTTTGTTCTCCATTCTAGTTTTGAGGCCCAAACCACACAGGGATTTGGCGAACATCATTCATTTCTCTTTATTTTTATTTACTTTATTTCATTATTGTTTTTATTTTATTTATTTTGTTTTCTTTGTGGGGATGGGAGACCAAACCTTGACTTCTAAGGAAAAAGACCATGCCAAATATCATTGGACTAACATCACTTTGGCTATTCATTTGCTTGTTAATGGTTTTTTTGATGTCCCTGTCATTCTTGGTCTTAATACATTTTGGAACTGAGAGACTTGTCTCATTTATTATCATTAATAAAGAGGATTTTTCCGTTCAAGAAAAAAAATACATTTTGGTACTGATATTCACAATTCCAAACAGAGCATGGAGAAGACATTCAAATTCTCCATTATGCAGTTGGGCAGAAGTATGATGCTCATTATGATTTCTTTGTTGATGAGTACAACCTCAAAAGTGTAGGCCAAAGAATGGCCACTCTCCTCATGTATTTGTAAGATTCTACTTTGACAATCTCTCAAACTCATCTACCCTCTTCTCTAGTTTGTCTCTGAATCGATACCAATACCAATGCAGGTCGGATGTTGAAGAGGGGGGCGAGACGGTGTTCCCCGCTGCAAAAGGAAACTTTAGCTCTGTGCCATGGTGGAATGAACTGTCTGAATGTGGTAAAAGTGGACTTTCTATAAAACCAAAAATGGGAGATGCTTTATTGTTCTGGAGCATGAAGCCCGATACTACCTTAGACCCTACAAGTTTACATGGTTAGTCCTTTTCATTTTCCTTCCTATTTTACTGTTTTCACGCATTATAATCTTATTCGTCTTTTCTTTTTTATTGATATGTAGGTGCCTGCCCTGTCATTAGAGGGAACAAATGGTCATGTACAAAGTGGATACATGTTAATAAATATATTTAA

mRNA sequence

ATGGCAATATCTATAGGGAAATACATCAAGTTACAGGGTAAGAAATGGTCCACATTCCAGCTTTCGAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTATTGCTCTTTGGTTCTTCTCTCCTCCTGAAACTTCTCACCACCGTCTCTCGTCCGTCCGGCATACAGCATTTTTAAGTGATGGGTTGGGGAAGAGAGGGGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTTTATCATAATTTCTTATCCAAGGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCTCACATGGAGAAATCAACTGTGGTTGATAGCGAAACTGGCAAGAGTGTGGATAGCAGTGTGCGAACCAGTTCTGGGATGTTTCTGAATAGAGGGCAGGACAAAATCATCAGTAACATAGAGAAAAGAATAGCAGATTTTACATTCATTCCTATAGAGCATGGAGAAGACATTCAAATTCTCCATTATGCAGTTGGGCAGAAGTATGATGCTCATTATGATTTCTTTGTTGATGAGTACAACCTCAAAAGTGTAGGCCAAAGAATGGCCACTCTCCTCATGTATTTGTCGGATGTTGAAGAGGGGGGCGAGACGGTGTTCCCCGCTGCAAAAGGAAACTTTAGCTCTGTGCCATGGTGGAATGAACTGTCTGAATGTGGTAAAAGTGGACTTTCTATAAAACCAAAAATGGGAGATGCTTTATTGTTCTGGAGCATGAAGCCCGATACTACCTTAGACCCTACAAGTTTACATGGTGCCTGCCCTGTCATTAGAGGGAACAAATGGTCATGTACAAAGTGGATACATGTTAATAAATATATTTAA

Coding sequence (CDS)

ATGGCAATATCTATAGGGAAATACATCAAGTTACAGGGTAAGAAATGGTCCACATTCCAGCTTTCGAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTATTGCTCTTTGGTTCTTCTCTCCTCCTGAAACTTCTCACCACCGTCTCTCGTCCGTCCGGCATACAGCATTTTTAAGTGATGGGTTGGGGAAGAGAGGGGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTTTATCATAATTTCTTATCCAAGGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCTCACATGGAGAAATCAACTGTGGTTGATAGCGAAACTGGCAAGAGTGTGGATAGCAGTGTGCGAACCAGTTCTGGGATGTTTCTGAATAGAGGGCAGGACAAAATCATCAGTAACATAGAGAAAAGAATAGCAGATTTTACATTCATTCCTATAGAGCATGGAGAAGACATTCAAATTCTCCATTATGCAGTTGGGCAGAAGTATGATGCTCATTATGATTTCTTTGTTGATGAGTACAACCTCAAAAGTGTAGGCCAAAGAATGGCCACTCTCCTCATGTATTTGTCGGATGTTGAAGAGGGGGGCGAGACGGTGTTCCCCGCTGCAAAAGGAAACTTTAGCTCTGTGCCATGGTGGAATGAACTGTCTGAATGTGGTAAAAGTGGACTTTCTATAAAACCAAAAATGGGAGATGCTTTATTGTTCTGGAGCATGAAGCCCGATACTACCTTAGACCCTACAAGTTTACATGGTGCCTGCCCTGTCATTAGAGGGAACAAATGGTCATGTACAAAGTGGATACATGTTAATAAATATATTTAA

Protein sequence

MAISIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSHHRLSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSVDSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFVDEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKMGDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI
Homology
BLAST of Pay0021069 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 4.2e-113
Identity = 199/282 (70.57%), Postives = 226/282 (80.14%), Query Frame = 0

Query: 7   KYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPP----ETSHHRLSSVRHTAF- 66
           ++ + Q +KWST  L  + M  +L +   ML+A   FS P    E+S   LS  R  A  
Sbjct: 5   RHSRFQARKWSTLML-VLFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRRAATE 64

Query: 67  LSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSVD 126
            S+GLGKRGDQW E +SWEPRAFVYHNFLSKEEC YLISLAKPHM KSTVVDSETGKS D
Sbjct: 65  RSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKD 124

Query: 127 SSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFVD 186
           S VRTSSG FL RG+DKII  IEKRIAD+TFIP +HGE +Q+LHY  GQKY+ HYD+FVD
Sbjct: 125 SRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVD 184

Query: 187 EYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKMG 246
           E+N K+ GQRMAT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELSECGK GLS+KP+MG
Sbjct: 185 EFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPRMG 244

Query: 247 DALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKY 284
           DALLFWSM+PD TLDPTSLHG CPVIRGNKWS TKW+HV +Y
Sbjct: 245 DALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

BLAST of Pay0021069 vs. ExPASy Swiss-Prot
Match: F4JZ24 (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 1.9e-102
Identity = 178/270 (65.93%), Postives = 212/270 (78.52%), Query Frame = 0

Query: 22  SKMIMALVLALGFFMLIALWF--FSPPET------SHHRLSSVRHTAFLSDGLGKRGDQW 81
           S ++ A+++   F +LI L F   S P        ++   S VR T   S     + ++W
Sbjct: 18  STLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKNERW 77

Query: 82  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSVDSSVRTSSGMFLN 141
           VE ISWEPRA VYHNFL+KEEC YLI LAKPHMEKSTVVD +TGKS DS VRTSSG FL 
Sbjct: 78  VEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGTFLA 137

Query: 142 RGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFVDEYNLKSVGQRMA 201
           RG+DK I  IEKRI+DFTFIP+EHGE +Q+LHY +GQKY+ HYD+F+DEYN ++ GQR+A
Sbjct: 138 RGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGGQRIA 197

Query: 202 TLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKMGDALLFWSMKPDT 261
           T+LMYLSDVEEGGETVFPAAKGN+S+VPWWNELSECGK GLS+KPKMGDALLFWSM PD 
Sbjct: 198 TVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKMGDALLFWSMTPDA 257

Query: 262 TLDPTSLHGACPVIRGNKWSCTKWIHVNKY 284
           TLDP+SLHG C VI+GNKWS TKW+ V++Y
Sbjct: 258 TLDPSSLHGGCAVIKGNKWSSTKWLRVHEY 287

BLAST of Pay0021069 vs. ExPASy Swiss-Prot
Match: F4JNU8 (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 4.6e-96
Identity = 164/283 (57.95%), Postives = 208/283 (73.50%), Query Frame = 0

Query: 10  KLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSHHR---------LSSVRHTA 69
           +L+ K   +F      + +++     +L+ L  FS P T+            + +++   
Sbjct: 7   QLRNKPRKSFSTQTFTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQTIQERE 66

Query: 70  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 129
              D     GD+W+E ISWEPRAFVYHNFL+ EEC +LISLAKP M KS VVD +TGKS+
Sbjct: 67  SFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVKTGKSI 126

Query: 130 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 189
           DS VRTSSG FLNRG D+I+  IE RI+DFTFIP E+GE +Q+LHY VGQ+Y+ H+D+F 
Sbjct: 127 DSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPHHDYFF 186

Query: 190 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 249
           DE+N++  GQR+AT+LMYLSDV+EGGETVFPAAKGN S VPWW+ELS+CGK GLS+ PK 
Sbjct: 187 DEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLSVLPKK 246

Query: 250 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKY 284
            DALLFWSMKPD +LDP+SLHG CPVI+GNKWS TKW HV++Y
Sbjct: 247 RDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

BLAST of Pay0021069 vs. ExPASy Swiss-Prot
Match: Q24JN5 (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.5e-94
Identity = 162/289 (56.06%), Postives = 213/289 (73.70%), Query Frame = 0

Query: 1   MAISIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSHHRLSS----- 60
           MA    ++++ Q +K  +       + ++L +   +L+ L   S P  + +   +     
Sbjct: 1   MASKSKQHLRYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTN 60

Query: 61  -VRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 120
            VR +   S      G++WVE ISWEPRA VYHNFL+ EEC +LISLAKP M KSTVVD 
Sbjct: 61  IVRKSETSSGDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDE 120

Query: 121 ETGKSVDSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDA 180
           +TG S DS VRTSSG FL RG D+++  IEKRI+DFTFIP+E+GE +Q+LHY VGQKY+ 
Sbjct: 121 KTGGSKDSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEP 180

Query: 181 HYDFFVDEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGL 240
           HYD+F+DE+N K+ GQR+AT+LMYLSDV++GGETVFPAA+GN S+VPWWNELS+CGK GL
Sbjct: 181 HYDYFLDEFNTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGL 240

Query: 241 SIKPKMGDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKY 284
           S+ PK  DALLFW+M+PD +LDP+SLHG CPV++GNKWS TKW HV+++
Sbjct: 241 SVLPKKRDALLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEF 289

BLAST of Pay0021069 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 4.2e-65
Identity = 113/207 (54.59%), Postives = 156/207 (75.36%), Query Frame = 0

Query: 77  ISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSVDSSVRTSSGMFLNRGQ 136
           +SW PR F+Y  FLS EEC + I LAK  +EKS V D+++G+SV+S VRTSSGMFL++ Q
Sbjct: 59  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQ 118

Query: 137 DKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFVDEYNLKSVGQRMATLL 196
           D I+SN+E ++A +TF+P E+GE +QILHY  GQKY+ H+D+F D+ NL+  G R+AT+L
Sbjct: 119 DDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVL 178

Query: 197 MYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKMGDALLFWSMKPDTTLD 256
           MYLS+VE+GGETVFP  KG  + +   +  +EC K G ++KP+ GDALLF+++ P+ T D
Sbjct: 179 MYLSNVEKGGETVFPMWKGKATQLK-DDSWTECAKQGYAVKPRKGDALLFFNLHPNATTD 238

Query: 257 PTSLHGACPVIRGNKWSCTKWIHVNKY 284
             SLHG+CPV+ G KWS T+WIHV  +
Sbjct: 239 SNSLHGSCPVVEGEKWSATRWIHVKSF 264

BLAST of Pay0021069 vs. ExPASy TrEMBL
Match: A0A1S4DZG7 (probable prolyl 4-hydroxylase 3 OS=Cucumis melo OX=3656 GN=LOC103496316 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.4e-164
Identity = 282/284 (99.30%), Postives = 283/284 (99.65%), Query Frame = 0

Query: 1   MAISIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSHHRLSSVRHTA 60
           MA+SIGKYIKLQGKKWSTFQLSKMIMALVLALGFFML ALWFFSPPETSHHRLSSVRHTA
Sbjct: 1   MAVSIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLSALWFFSPPETSHHRLSSVRHTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120
           FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV
Sbjct: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120

Query: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180
           DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV
Sbjct: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180

Query: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240
           DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM
Sbjct: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240

Query: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI 285
           GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI
Sbjct: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI 284

BLAST of Pay0021069 vs. ExPASy TrEMBL
Match: A0A1S3C2P6 (probable prolyl 4-hydroxylase 3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496331 PE=4 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.3e-154
Identity = 265/284 (93.31%), Postives = 272/284 (95.77%), Query Frame = 0

Query: 1   MAISIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSHHRLSSVRHTA 60
           MA+S GKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIAL FFSPPETSHHRL SVR TA
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLPSVRRTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120
           F SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS+TGKSV
Sbjct: 61  FQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSV 120

Query: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180
           DS VRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGE +QILHY VGQKYDAHYD+FV
Sbjct: 121 DSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240
           DEYN+K  GQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGK GLS+KPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI 285
           GDALLFWSMKPD TLDPTSLHGACPVIRGNKWSCTKW+HVNKYI
Sbjct: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVNKYI 284

BLAST of Pay0021069 vs. ExPASy TrEMBL
Match: A0A5D3BGX5 (Putative prolyl 4-hydroxylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001170 PE=4 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.1e-148
Identity = 260/262 (99.24%), Postives = 261/262 (99.62%), Query Frame = 0

Query: 1   MAISIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSHHRLSSVRHTA 60
           MA+SIGKYIKLQGKKWSTFQLSKMIMALVLALGFFML ALWFFSPPETSHHRLSSVRHTA
Sbjct: 1   MAVSIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLSALWFFSPPETSHHRLSSVRHTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120
           FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV
Sbjct: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120

Query: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180
           DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV
Sbjct: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180

Query: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240
           DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM
Sbjct: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240

Query: 241 GDALLFWSMKPDTTLDPTSLHG 263
           GDALLFWSMKPDTTLDPTSLHG
Sbjct: 241 GDALLFWSMKPDTTLDPTSLHG 262

BLAST of Pay0021069 vs. ExPASy TrEMBL
Match: A0A0A0LFF5 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009620 PE=4 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.9e-140
Identity = 240/259 (92.66%), Postives = 248/259 (95.75%), Query Frame = 0

Query: 26  MALVLALGFFMLIALWFFSPPETSHHRLSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV 85
           MALVLALGFFMLIAL F SPPETSHHR SSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV
Sbjct: 1   MALVLALGFFMLIALRFLSPPETSHHRFSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV 60

Query: 86  YHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSVDSSVRTSSGMFLNRGQDKIISNIEK 145
           YHNFLSKEECLYLISLAKPHMEKSTVVDS+TG+SVDS VRTSSGMFLNRGQDKII NIEK
Sbjct: 61  YHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEK 120

Query: 146 RIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFVDEYNLKSVGQRMATLLMYLSDVEEG 205
           RIADFTFIPIEHGE +QILHY VGQKYDAHYD+FVDEYN+K  GQRMATLLMYLSDVEEG
Sbjct: 121 RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEG 180

Query: 206 GETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKMGDALLFWSMKPDTTLDPTSLHGACP 265
           GETVFPAAKGNFSSVPWWNELSECGK GLS+KPKMGDALLFWSMKPD TLDPTSLHGACP
Sbjct: 181 GETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSLHGACP 240

Query: 266 VIRGNKWSCTKWIHVNKYI 285
           VIRGNKWSCTKW+HV+KYI
Sbjct: 241 VIRGNKWSCTKWMHVDKYI 259

BLAST of Pay0021069 vs. ExPASy TrEMBL
Match: A0A5D3BDQ0 (Putative prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001180 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 4.6e-139
Identity = 244/262 (93.13%), Postives = 250/262 (95.42%), Query Frame = 0

Query: 1   MAISIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSHHRLSSVRHTA 60
           MA+S GKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIAL FFSPPETSHHRL SVR TA
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLPSVRRTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120
           F SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS+TGKSV
Sbjct: 61  FQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSV 120

Query: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180
           DS VRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGE +QILHY VGQKYDAHYD+FV
Sbjct: 121 DSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240
           DEYN+K  GQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGK GLS+KPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDTTLDPTSLHG 263
           GDALLFWSMKPD TLDPTSLHG
Sbjct: 241 GDALLFWSMKPDATLDPTSLHG 262

BLAST of Pay0021069 vs. NCBI nr
Match: XP_016901368.1 (PREDICTED: probable prolyl 4-hydroxylase 3 [Cucumis melo])

HSP 1 Score: 588.6 bits (1516), Expect = 2.9e-164
Identity = 282/284 (99.30%), Postives = 283/284 (99.65%), Query Frame = 0

Query: 1   MAISIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSHHRLSSVRHTA 60
           MA+SIGKYIKLQGKKWSTFQLSKMIMALVLALGFFML ALWFFSPPETSHHRLSSVRHTA
Sbjct: 1   MAVSIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLSALWFFSPPETSHHRLSSVRHTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120
           FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV
Sbjct: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120

Query: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180
           DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV
Sbjct: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180

Query: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240
           DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM
Sbjct: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240

Query: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI 285
           GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI
Sbjct: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI 284

BLAST of Pay0021069 vs. NCBI nr
Match: XP_008456388.1 (PREDICTED: probable prolyl 4-hydroxylase 3 isoform X2 [Cucumis melo])

HSP 1 Score: 555.4 bits (1430), Expect = 2.8e-154
Identity = 265/284 (93.31%), Postives = 272/284 (95.77%), Query Frame = 0

Query: 1   MAISIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSHHRLSSVRHTA 60
           MA+S GKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIAL FFSPPETSHHRL SVR TA
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLPSVRRTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120
           F SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS+TGKSV
Sbjct: 61  FQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSV 120

Query: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180
           DS VRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGE +QILHY VGQKYDAHYD+FV
Sbjct: 121 DSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240
           DEYN+K  GQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGK GLS+KPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI 285
           GDALLFWSMKPD TLDPTSLHGACPVIRGNKWSCTKW+HVNKYI
Sbjct: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVNKYI 284

BLAST of Pay0021069 vs. NCBI nr
Match: XP_011648735.2 (probable prolyl 4-hydroxylase 3 [Cucumis sativus] >KAE8651549.1 hypothetical protein Csa_019368 [Cucumis sativus])

HSP 1 Score: 555.1 bits (1429), Expect = 3.6e-154
Identity = 264/284 (92.96%), Postives = 273/284 (96.13%), Query Frame = 0

Query: 1   MAISIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSHHRLSSVRHTA 60
           MAIS GKYIKLQG+KWSTFQLSKMIMALVLALGFFMLIAL FFSPPETSHHR SSVRHTA
Sbjct: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSSVRHTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120
           FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS+TG+SV
Sbjct: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120

Query: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180
           DS VRTSSGMFLNRGQDKII NIEKRIADFTFIPIEHGE +QILHY VGQKYDAHYD+FV
Sbjct: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240
           DEYN+K  GQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGK GLS+KPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI 285
           GDALLFWSMKPD TLDPTSLHGACPVIRGNKWSCTKW+HV+KYI
Sbjct: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI 284

BLAST of Pay0021069 vs. NCBI nr
Match: XP_011650099.2 (probable prolyl 4-hydroxylase 3 [Cucumis sativus] >XP_031736348.1 probable prolyl 4-hydroxylase 3 [Cucumis sativus])

HSP 1 Score: 552.0 bits (1421), Expect = 3.0e-153
Identity = 261/282 (92.55%), Postives = 272/282 (96.45%), Query Frame = 0

Query: 1   MAISIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSHHRLSSVRHTA 60
           MA+SI KYIKLQGKKWSTFQLSKMIMALVLALGFFMLIAL F SPPETSHHR SSVRHTA
Sbjct: 1   MAVSIRKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPPETSHHRFSSVRHTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120
           FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVD+ETGK+V
Sbjct: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNV 120

Query: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180
           + SVRTSSGMFLNRGQDKI+SNIEKRIADFTFIPIEHGE +QILHY VGQKYDAHYDFF 
Sbjct: 121 EDSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFD 180

Query: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240
           DE+NLK +GQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELS+CGK GLS+KPKM
Sbjct: 181 DEFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNK 283
           GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVN+
Sbjct: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 282

BLAST of Pay0021069 vs. NCBI nr
Match: XP_038889689.1 (probable prolyl 4-hydroxylase 3 [Benincasa hispida])

HSP 1 Score: 540.8 bits (1392), Expect = 7.0e-150
Identity = 258/288 (89.58%), Postives = 271/288 (94.10%), Query Frame = 0

Query: 1   MAISIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSH----HRLSSV 60
           MA+S GKY K+QGKKWSTF+LSKMIMALVLALGFFML+AL FFSPPETSH    H L+SV
Sbjct: 1   MALSKGKYTKIQGKKWSTFELSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHHLASV 60

Query: 61  RHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSET 120
           RH+A  SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS+T
Sbjct: 61  RHSAVESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 121 GKSVDSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHY 180
           GKSVDS VRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGE +QILHY VGQKYDAHY
Sbjct: 121 GKSVDSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 180

Query: 181 DFFVDEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSI 240
           D+FVDEYN+K  GQRMATLLMYLSDVEEGGETVFPAA+GNFSSVPWWNELSECGK GLS+
Sbjct: 181 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAARGNFSSVPWWNELSECGKGGLSV 240

Query: 241 KPKMGDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI 285
           KPKMGDALLFWSMKPD TLDPTSLHGACPVIRGNKWSCTKW+HVNKYI
Sbjct: 241 KPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVNKYI 288

BLAST of Pay0021069 vs. TAIR 10
Match: AT1G20270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 409.1 bits (1050), Expect = 3.0e-114
Identity = 199/282 (70.57%), Postives = 226/282 (80.14%), Query Frame = 0

Query: 7   KYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPP----ETSHHRLSSVRHTAF- 66
           ++ + Q +KWST  L  + M  +L +   ML+A   FS P    E+S   LS  R  A  
Sbjct: 5   RHSRFQARKWSTLML-VLFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRRAATE 64

Query: 67  LSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSVD 126
            S+GLGKRGDQW E +SWEPRAFVYHNFLSKEEC YLISLAKPHM KSTVVDSETGKS D
Sbjct: 65  RSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKD 124

Query: 127 SSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFVD 186
           S VRTSSG FL RG+DKII  IEKRIAD+TFIP +HGE +Q+LHY  GQKY+ HYD+FVD
Sbjct: 125 SRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVD 184

Query: 187 EYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKMG 246
           E+N K+ GQRMAT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELSECGK GLS+KP+MG
Sbjct: 185 EFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPRMG 244

Query: 247 DALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKY 284
           DALLFWSM+PD TLDPTSLHG CPVIRGNKWS TKW+HV +Y
Sbjct: 245 DALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

BLAST of Pay0021069 vs. TAIR 10
Match: AT5G66060.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 373.6 bits (958), Expect = 1.4e-103
Identity = 178/270 (65.93%), Postives = 212/270 (78.52%), Query Frame = 0

Query: 22  SKMIMALVLALGFFMLIALWF--FSPPET------SHHRLSSVRHTAFLSDGLGKRGDQW 81
           S ++ A+++   F +LI L F   S P        ++   S VR T   S     + ++W
Sbjct: 18  STLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKNERW 77

Query: 82  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSVDSSVRTSSGMFLN 141
           VE ISWEPRA VYHNFL+KEEC YLI LAKPHMEKSTVVD +TGKS DS VRTSSG FL 
Sbjct: 78  VEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGTFLA 137

Query: 142 RGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFVDEYNLKSVGQRMA 201
           RG+DK I  IEKRI+DFTFIP+EHGE +Q+LHY +GQKY+ HYD+F+DEYN ++ GQR+A
Sbjct: 138 RGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGGQRIA 197

Query: 202 TLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKMGDALLFWSMKPDT 261
           T+LMYLSDVEEGGETVFPAAKGN+S+VPWWNELSECGK GLS+KPKMGDALLFWSM PD 
Sbjct: 198 TVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKMGDALLFWSMTPDA 257

Query: 262 TLDPTSLHGACPVIRGNKWSCTKWIHVNKY 284
           TLDP+SLHG C VI+GNKWS TKW+ V++Y
Sbjct: 258 TLDPSSLHGGCAVIKGNKWSSTKWLRVHEY 287

BLAST of Pay0021069 vs. TAIR 10
Match: AT4G35810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 352.4 bits (903), Expect = 3.3e-97
Identity = 164/283 (57.95%), Postives = 208/283 (73.50%), Query Frame = 0

Query: 10  KLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSHHR---------LSSVRHTA 69
           +L+ K   +F      + +++     +L+ L  FS P T+            + +++   
Sbjct: 7   QLRNKPRKSFSTQTFTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQTIQERE 66

Query: 70  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 129
              D     GD+W+E ISWEPRAFVYHNFL+ EEC +LISLAKP M KS VVD +TGKS+
Sbjct: 67  SFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVKTGKSI 126

Query: 130 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 189
           DS VRTSSG FLNRG D+I+  IE RI+DFTFIP E+GE +Q+LHY VGQ+Y+ H+D+F 
Sbjct: 127 DSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPHHDYFF 186

Query: 190 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 249
           DE+N++  GQR+AT+LMYLSDV+EGGETVFPAAKGN S VPWW+ELS+CGK GLS+ PK 
Sbjct: 187 DEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLSVLPKK 246

Query: 250 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKY 284
            DALLFWSMKPD +LDP+SLHG CPVI+GNKWS TKW HV++Y
Sbjct: 247 RDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

BLAST of Pay0021069 vs. TAIR 10
Match: AT2G17720.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 346.7 bits (888), Expect = 1.8e-95
Identity = 162/289 (56.06%), Postives = 213/289 (73.70%), Query Frame = 0

Query: 1   MAISIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALWFFSPPETSHHRLSS----- 60
           MA    ++++ Q +K  +       + ++L +   +L+ L   S P  + +   +     
Sbjct: 1   MASKSKQHLRYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTN 60

Query: 61  -VRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 120
            VR +   S      G++WVE ISWEPRA VYHNFL+ EEC +LISLAKP M KSTVVD 
Sbjct: 61  IVRKSETSSGDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDE 120

Query: 121 ETGKSVDSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDA 180
           +TG S DS VRTSSG FL RG D+++  IEKRI+DFTFIP+E+GE +Q+LHY VGQKY+ 
Sbjct: 121 KTGGSKDSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEP 180

Query: 181 HYDFFVDEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGL 240
           HYD+F+DE+N K+ GQR+AT+LMYLSDV++GGETVFPAA+GN S+VPWWNELS+CGK GL
Sbjct: 181 HYDYFLDEFNTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGL 240

Query: 241 SIKPKMGDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKY 284
           S+ PK  DALLFW+M+PD +LDP+SLHG CPV++GNKWS TKW HV+++
Sbjct: 241 SVLPKKRDALLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEF 289

BLAST of Pay0021069 vs. TAIR 10
Match: AT5G66060.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 276.6 bits (706), Expect = 2.3e-74
Identity = 139/220 (63.18%), Postives = 166/220 (75.45%), Query Frame = 0

Query: 22  SKMIMALVLALGFFMLIALWF--FSPPET------SHHRLSSVRHTAFLSDGLGKRGDQW 81
           S ++ A+++   F +LI L F   S P        ++   S VR T   S     + ++W
Sbjct: 18  STLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKNERW 77

Query: 82  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSVDSSVRTSSGMFLN 141
           VE ISWEPRA VYHNFL  EEC YLI LAKPHMEKSTVVD +TGKS DS VRTSSG FL 
Sbjct: 78  VEIISWEPRASVYHNFL--EECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGTFLA 137

Query: 142 RGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFVDEYNLKSVGQRMA 201
           RG+DK I  IEKRI+DFTFIP+EHGE +Q+LHY +GQKY+ HYD+F+DEYN ++ GQR+A
Sbjct: 138 RGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGGQRIA 197

Query: 202 TLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSG 234
           T+LMYLSDVEEGGETVFPAAKGN+S+VPWWNELSECGK G
Sbjct: 198 TVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGG 235

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LN204.2e-11370.57Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=... [more]
F4JZ241.9e-10265.93Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 S... [more]
F4JNU84.6e-9657.95Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=... [more]
Q24JN52.5e-9456.06Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1[more]
Q8L9704.2e-6554.59Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A1S4DZG71.4e-16499.30probable prolyl 4-hydroxylase 3 OS=Cucumis melo OX=3656 GN=LOC103496316 PE=4 SV=... [more]
A0A1S3C2P61.3e-15493.31probable prolyl 4-hydroxylase 3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034963... [more]
A0A5D3BGX51.1e-14899.24Putative prolyl 4-hydroxylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A0A0LFF51.9e-14092.66Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G... [more]
A0A5D3BDQ04.6e-13993.13Putative prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo var. makuwa OX=119469... [more]
Match NameE-valueIdentityDescription
XP_016901368.12.9e-16499.30PREDICTED: probable prolyl 4-hydroxylase 3 [Cucumis melo][more]
XP_008456388.12.8e-15493.31PREDICTED: probable prolyl 4-hydroxylase 3 isoform X2 [Cucumis melo][more]
XP_011648735.23.6e-15492.96probable prolyl 4-hydroxylase 3 [Cucumis sativus] >KAE8651549.1 hypothetical pro... [more]
XP_011650099.23.0e-15392.55probable prolyl 4-hydroxylase 3 [Cucumis sativus] >XP_031736348.1 probable proly... [more]
XP_038889689.17.0e-15089.58probable prolyl 4-hydroxylase 3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G20270.13.0e-11470.572-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G66060.11.4e-10365.932-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G35810.13.3e-9757.952-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G17720.11.8e-9556.062-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G66060.22.3e-7463.182-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 81..279
e-value: 2.9E-65
score: 232.8
IPR044862Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domainPFAMPF136402OG-FeII_Oxy_3coord: 162..279
e-value: 3.5E-20
score: 72.7
NoneNo IPR availableGENE3D2.60.120.620q2cbj1_9rhob like domaincoord: 74..280
e-value: 1.4E-78
score: 265.4
NoneNo IPR availablePANTHERPTHR10869:SF169PROLYL 4-HYDROXYLASE 3-RELATEDcoord: 6..283
IPR045054Prolyl 4-hydroxylasePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 6..283
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 157..280
score: 11.767708

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0021069.1Pay0021069.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen