Pay0020719 (gene) Melon (Payzawat) v1

Overview
NamePay0020719
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionMACPF domain-containing protein
Locationchr12: 22617638 .. 22621552 (-)
RNA-Seq ExpressionPay0020719
SyntenyPay0020719
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTTCAACATCAACCGGTTCAATCCTCAATCAGCCGCCGAGAAAGCGGTTTCAGTTATCGGCTTCGGCTACGATCTCTGTAACGATTTGCGATTGTCTTCTTGCAAGCCGGGTCCATCGGGGTCTAAGCTGATCGATATCGACTTCACTCGAAGTAGAGACCTTGTACTTCCGGCCGGCGTTGTCGTCCCTAATGTTCCTACTTCCATCAACTGTGATAAAGGCGAGCGTACCCGATTCCGGTCGGATGTCATCTCCTTCAATCAGGTAAATTTCTTTGAAATGGGTTTTTGCTGTCTTTAGCTTAGAATTCTCTTCGTTTTCTTGTTTTTTTTTTTTCATTTGTTGGCTTCATAGAACGAACTGGGTTTGTTTTTTTTCCGGTTAAAAACAGATGTCGGAGCTGTTTAATCAGCAATTATCTCTATCTGGGAAAATTCCGTCGGGGTTCTTTAACACCATGTTTGGATTAAAGGGTTGCTGGCAAAACGATGCAGGTTCCACAAGAAGTCTTGCATTTGATGGTTGTTTCATTACACTTTATAACATTGAGTTGGAAAAATCTCATATAACTCTCTCTGAGCAAGTCAAAAGGGAGGTTCCTTCTTCATGGGATCCTGCTGCCCTTGCAGAGTATGTTTCTTTTGTTTCTGTTTTTTTGTCTTTTCTTTAGTAGAATTCTAGACATTTGGATGTTTGTCTGTCTGGACTGAAAGTCTCTTCTATTGAAATATCTTCATCACTGATATTTAACGAACTTCTCTGTGTTCATCTTCTCCACATTAACACACTAATTGTTAATGGTGTTGAATGAATGAGAATGGATTTTTGGAAGTAATGCTTTCAACCATGCGCTTCTCTGTCCAACACTTGAGGAAGGACTGGTCTTTGAAATTGCTTTGACTATGTTGTCAAAAAGGTGGAAAATGTGCTTTTTGTTAGCTACTTTTAAGTTTTACTATCCCAAAAGTAAATAAAAACAGTGATGATGAGAATCTTTCTTGAAAGGATGGCCTTGTATGAACCTATTCTTTTAAATCCTTCGCATCTTCCCCTTTGTCCTAACAATCATTAGGTCATTTCATGGTCGTCAGTTCTTCTGAACTTCATAACTACCAGCTTAAAGGATGATCTGATTAGTATAATCCCTTGGATTCTTCTAATATATCATACAAAGACACATTTTGTTCACGAGTTCTTTGGTTTAGATTTTACCCAAGTGCTTGTTGGGTACGTCTCGTAATTACCAATATGTGTTCATGTTGCATTTGAAACATTGTTTATCGAATACATTACACAAACACTATCACATTAGACATTAGGGACATGTCAAATACTTGTTAAAGTGAAACACATTGGATATCTGATTTTTATGCCAACTAAGTACTAACTTGATTTCAGGTTCATTGAGAAATATGGTACTCATGTGGTTGTGGGTGTAAAAATGGGAGGAAAAGATGTCATTTTCATGAGGCAATTACGAGAATCGAATGTAGAGCAAACCGATGTGCAGAAGAAGTTAAAGAAATTAGCAGATGATAAATTTTCTGAAGATGCAAATGGAGGTTTCACGTCCAATTCTGTTGCAGCAAAAATCAAGGTACATACTCAAGAACATAAATGGCTATCACCATTTGGTCCATTCCATTGGTCCCATGTCAAAATAGAGAATGCTGATTAGCTATTGACGGAAATTAAAGAATCAATTTTCCTTGAGTCATGCATTATATGCAGCTCTTGATTCATGTTCTTTCCTTAAAATTCTTAGATTGAGATAAATCATATCAATAGAATATGGTTTAGTTGCTAAGTTCGCTATAGAATGTGGTTTAATTGTTACCTGATGTAGCAGTTGTCTTGCCTTCATCGTCACCTTGGCTTGCTTGTGAATTCTTCTTCTACTTCTGCATCTACACATATACTAAATATAACTCAACCAAGATTTATAAAAACTCAATAAACATGACTAGTCAGACAATGATTTTTGCTAATCGTATTCTAAATTAATCATTATAACCATGGGGCATACAATTTCTGATCCAATAGATTATTCTTTTTCTAAAACAATTTTGATAGGATTAATGATTCGCAATGGCATATTATTCAAATAGAACAGAAAAATTTAGAACTGCAAACAAGTTTTGTGGTTTGATGATGCATCTTCTAACTACATTTTCTATACGTATAAACATTTTAAGTCTCATCAGGAGAAATAAACTTTTATCCTCAATGATCCCAATTTTTAGGATGAATATTCTCTGCCATGGGAGCTTCGTAATGCATTTGCTGCCTCTATCAAACCACCAGTGATCAATCACTCAAAGGGTGATGTAAGCATCTCGGCATCTACCTGTTGTTCGTTTGGGGAGAATATTGCTACCCTATCGACCTATTAAGCAAATGAGAACTGACTTATTTAATTAAATGACTGGATGCAGGAGTTAACAGCGATTTATATTAGGAGAGGAGGTGTTGATGTTGGTCAAAGTCATAATCAATGGCTCTCAACCATTTCTCAGTCACCTAATGTTATCTCGATGTCCTTCGTACCAATAACATCACTCATGAATGGCGTCCGAGGCAATGGATTTCTAAGCCATGCAGTGAACCTGTATCTAAGATGTAAGTATGCAAGTCTTGCTTTTAAGTGTTGTTTCTCTACAGCCAATGGACCGAGGATAATTTTTGTCCTTTAATGAGAACCTGAAATATGGAGATCAGATACGTACATTATTTGCCCTTAAAATTTGACCTTAGTTACTCTTCCCATGGTAGCTGTAGTACTTGAGCTTCCACTATAAGATTTTGACCCTATGTTCATGCCAACAAGGTACCATTCACATTTAGTATTAGATTTTGATCATTGAAAAATTGTGCAGATAAACCTCCAATTGAGGAGCTCCAGCAATTTCTTGAATTTCAATTGCCTAGACAATGGGCGCCAGTGTACGCTGATCTTCCTCTTGCTCCTCGGCACAGGAAACAAGCCTCTCCATCTCTCAAGTTTACTTTGATGGGGCCCAAGCTTTATGTAAATACTACTAAGGTAATCGTCTGACTAGAATATTTGGGTCAGTTAAAAACTTAAAATAGCAATGGCACTGCCGCAAATGTGATAACTGCGGTTGGTTTTTGCCTGACCTATCTATTACAGAAAGGTTCCCTTTGCTTGGATGCAAATTGTTGAATGTTTCACATGGGGTCACAATTTTGAAACTGCAGGTTGATTCAAACAATCGTCCAGTAACGGGAGTTCGCTTGTTTCTGGAAGGAAAGAAAAGTGACCATCTGGCAATTCATCTCCAGCATCTATCAACTCTTCCCAAGACCCTTCAACTCTCAGATGATCTAAGCTATCGACCCATTGACGAGCCAGAGGAGAAGGGCTACTTCGAACCAGTTAAATGGAGCATATTCTCTCATGTCTGCACTGCGCCAGTAGAGTACCATGATTCTCTAATTGATGATGACTCTGCTTCTATCGTCACAAAGGCATGGTTTGAAGTTAAGGTTATTGGAATGAGAAAGGTCTTGTTCCTGAGGCTCGGGTTTTCGATGGTGGCATCAGCAGGGATCCGTAGATCGGACTGGGAAGGACCTTCAACAACTTCCCGGAAATCTGGACTCATCTCTACCTTTATCAGCACAAGGTTTAGTGCTGGGTTGCATCAACCAGAAGAGCAACCAAAAATTGAACTGAATTCGGCTGTTTATCCTGATGGTCCACCATTGACTTCTAGGGCAATGAAAATGTTGAATGTCATTGATACAAAAGAAATGGTGCGCGGTCCTGAGAATTCACCTGGTTATTGGGTGGTTACCGGAGCCAAGCTGTGCGTGGAAGGTGGCCGGATCTCTATCAAAGTGAAATACTCATTGTTAACCATATTGTCGGAGGATTCTATGATCTGA

mRNA sequence

ATGTCTTTCAACATCAACCGGTTCAATCCTCAATCAGCCGCCGAGAAAGCGGTTTCAGTTATCGGCTTCGGCTACGATCTCTGTAACGATTTGCGATTGTCTTCTTGCAAGCCGGGTCCATCGGGGTCTAAGCTGATCGATATCGACTTCACTCGAAGTAGAGACCTTGTACTTCCGGCCGGCGTTGTCGTCCCTAATGTTCCTACTTCCATCAACTGTGATAAAGGCGAGCGTACCCGATTCCGGTCGGATGTCATCTCCTTCAATCAGATGTCGGAGCTGTTTAATCAGCAATTATCTCTATCTGGGAAAATTCCGTCGGGGTTCTTTAACACCATGTTTGGATTAAAGGGTTGCTGGCAAAACGATGCAGGTTCCACAAGAAGTCTTGCATTTGATGGTTGTTTCATTACACTTTATAACATTGAGTTGGAAAAATCTCATATAACTCTCTCTGAGCAAGTCAAAAGGGAGGTTCCTTCTTCATGGGATCCTGCTGCCCTTGCAGAGTTCATTGAGAAATATGGTACTCATGTGGTTGTGGGTGTAAAAATGGGAGGAAAAGATGTCATTTTCATGAGGCAATTACGAGAATCGAATGTAGAGCAAACCGATGTGCAGAAGAAGTTAAAGAAATTAGCAGATGATAAATTTTCTGAAGATGCAAATGGAGGTTTCACGTCCAATTCTGTTGCAGCAAAAATCAAGGATGAATATTCTCTGCCATGGGAGCTTCGTAATGCATTTGCTGCCTCTATCAAACCACCAGTGATCAATCACTCAAAGGGTGATGAGTTAACAGCGATTTATATTAGGAGAGGAGGTGTTGATGTTGGTCAAAGTCATAATCAATGGCTCTCAACCATTTCTCAGTCACCTAATGTTATCTCGATGTCCTTCGTACCAATAACATCACTCATGAATGGCGTCCGAGGCAATGGATTTCTAAGCCATGCAGTGAACCTGTATCTAAGATATAAACCTCCAATTGAGGAGCTCCAGCAATTTCTTGAATTTCAATTGCCTAGACAATGGGCGCCAGTGTACGCTGATCTTCCTCTTGCTCCTCGGCACAGGAAACAAGCCTCTCCATCTCTCAAGTTTACTTTGATGGGGCCCAAGCTTTATGTAAATACTACTAAGGTTGATTCAAACAATCGTCCAGTAACGGGAGTTCGCTTGTTTCTGGAAGGAAAGAAAAGTGACCATCTGGCAATTCATCTCCAGCATCTATCAACTCTTCCCAAGACCCTTCAACTCTCAGATGATCTAAGCTATCGACCCATTGACGAGCCAGAGGAGAAGGGCTACTTCGAACCAGTTAAATGGAGCATATTCTCTCATGTCTGCACTGCGCCAGTAGAGTACCATGATTCTCTAATTGATGATGACTCTGCTTCTATCGTCACAAAGGCATGGTTTGAAGTTAAGGTTATTGGAATGAGAAAGGTCTTGTTCCTGAGGCTCGGGTTTTCGATGGTGGCATCAGCAGGGATCCGTAGATCGGACTGGGAAGGACCTTCAACAACTTCCCGGAAATCTGGACTCATCTCTACCTTTATCAGCACAAGGTTTAGTGCTGGGTTGCATCAACCAGAAGAGCAACCAAAAATTGAACTGAATTCGGCTGTTTATCCTGATGGTCCACCATTGACTTCTAGGGCAATGAAAATGTTGAATGTCATTGATACAAAAGAAATGGTGCGCGGTCCTGAGAATTCACCTGGTTATTGGGTGGTTACCGGAGCCAAGCTGTGCGTGGAAGGTGGCCGGATCTCTATCAAAGTGAAATACTCATTGTTAACCATATTGTCGGAGGATTCTATGATCTGA

Coding sequence (CDS)

ATGTCTTTCAACATCAACCGGTTCAATCCTCAATCAGCCGCCGAGAAAGCGGTTTCAGTTATCGGCTTCGGCTACGATCTCTGTAACGATTTGCGATTGTCTTCTTGCAAGCCGGGTCCATCGGGGTCTAAGCTGATCGATATCGACTTCACTCGAAGTAGAGACCTTGTACTTCCGGCCGGCGTTGTCGTCCCTAATGTTCCTACTTCCATCAACTGTGATAAAGGCGAGCGTACCCGATTCCGGTCGGATGTCATCTCCTTCAATCAGATGTCGGAGCTGTTTAATCAGCAATTATCTCTATCTGGGAAAATTCCGTCGGGGTTCTTTAACACCATGTTTGGATTAAAGGGTTGCTGGCAAAACGATGCAGGTTCCACAAGAAGTCTTGCATTTGATGGTTGTTTCATTACACTTTATAACATTGAGTTGGAAAAATCTCATATAACTCTCTCTGAGCAAGTCAAAAGGGAGGTTCCTTCTTCATGGGATCCTGCTGCCCTTGCAGAGTTCATTGAGAAATATGGTACTCATGTGGTTGTGGGTGTAAAAATGGGAGGAAAAGATGTCATTTTCATGAGGCAATTACGAGAATCGAATGTAGAGCAAACCGATGTGCAGAAGAAGTTAAAGAAATTAGCAGATGATAAATTTTCTGAAGATGCAAATGGAGGTTTCACGTCCAATTCTGTTGCAGCAAAAATCAAGGATGAATATTCTCTGCCATGGGAGCTTCGTAATGCATTTGCTGCCTCTATCAAACCACCAGTGATCAATCACTCAAAGGGTGATGAGTTAACAGCGATTTATATTAGGAGAGGAGGTGTTGATGTTGGTCAAAGTCATAATCAATGGCTCTCAACCATTTCTCAGTCACCTAATGTTATCTCGATGTCCTTCGTACCAATAACATCACTCATGAATGGCGTCCGAGGCAATGGATTTCTAAGCCATGCAGTGAACCTGTATCTAAGATATAAACCTCCAATTGAGGAGCTCCAGCAATTTCTTGAATTTCAATTGCCTAGACAATGGGCGCCAGTGTACGCTGATCTTCCTCTTGCTCCTCGGCACAGGAAACAAGCCTCTCCATCTCTCAAGTTTACTTTGATGGGGCCCAAGCTTTATGTAAATACTACTAAGGTTGATTCAAACAATCGTCCAGTAACGGGAGTTCGCTTGTTTCTGGAAGGAAAGAAAAGTGACCATCTGGCAATTCATCTCCAGCATCTATCAACTCTTCCCAAGACCCTTCAACTCTCAGATGATCTAAGCTATCGACCCATTGACGAGCCAGAGGAGAAGGGCTACTTCGAACCAGTTAAATGGAGCATATTCTCTCATGTCTGCACTGCGCCAGTAGAGTACCATGATTCTCTAATTGATGATGACTCTGCTTCTATCGTCACAAAGGCATGGTTTGAAGTTAAGGTTATTGGAATGAGAAAGGTCTTGTTCCTGAGGCTCGGGTTTTCGATGGTGGCATCAGCAGGGATCCGTAGATCGGACTGGGAAGGACCTTCAACAACTTCCCGGAAATCTGGACTCATCTCTACCTTTATCAGCACAAGGTTTAGTGCTGGGTTGCATCAACCAGAAGAGCAACCAAAAATTGAACTGAATTCGGCTGTTTATCCTGATGGTCCACCATTGACTTCTAGGGCAATGAAAATGTTGAATGTCATTGATACAAAAGAAATGGTGCGCGGTCCTGAGAATTCACCTGGTTATTGGGTGGTTACCGGAGCCAAGCTGTGCGTGGAAGGTGGCCGGATCTCTATCAAAGTGAAATACTCATTGTTAACCATATTGTCGGAGGATTCTATGATCTGA

Protein sequence

MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIGMRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLLTILSEDSMI
Homology
BLAST of Pay0020719 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 1.3e-225
Identity = 391/612 (63.89%), Postives = 475/612 (77.61%), Query Frame = 0

Query: 4   NINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVV 63
           N  R +  SAAEKAVSVIG GYDLC+D+R S+CK  P GS+L++ID TR+RDL+ P G+V
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQND 123
           V NV +SI CDKGERTR RSD++SFNQMSE FNQ + LSGKIPSG FN MF    CW  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGV 183
           A S ++LA+DG FI+LY++E+ +  +TL ++VKREVPSSWD AALA FIEKYGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSV-AAKIKDEYSLP 243
            MGGKDVI ++Q+R+SN E  ++QK LK   D++F  D     +  SV + K K+E  L 
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQ 244

Query: 244 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVP 303
           W L+  F  S+   V+ H+K +E+  + IRRGGVD+GQSH +WLST+SQ+PNVISM FVP
Sbjct: 245 WGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVP 304

Query: 304 ITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQA 363
           ITSL++G+ G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  R  KQ+
Sbjct: 305 ITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQS 364

Query: 364 SPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSD 423
           SPSL+F+LMGPKLYVNT+KVDS  RPVTG+R FLEGKK +HLAIHLQHLS  P +L LS 
Sbjct: 365 SPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSH 424

Query: 424 DLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIGMR 483
           D +Y PI+EP EKGY+ PVKW IFSHVCT PV+Y+ +   DD+ASIVTKAW EVK +GMR
Sbjct: 425 DDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKGMGMR 484

Query: 484 KVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLH----QPEEQPKI 543
           KVLFLRLGFS+ ASA  R+S W+  ST SRKSG+ S  ISTR S GL       + Q KI
Sbjct: 485 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKI 544

Query: 544 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 603
           ++NSAVYP GP    +  K+L+++DTKE++RGPE  PGYWVVTGAKLCVE G+ISIK KY
Sbjct: 545 DINSAVYPRGPSPPVKP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKY 604

Query: 604 SLLTILSEDSMI 611
           SLLT++SEDS++
Sbjct: 605 SLLTVISEDSLV 612

BLAST of Pay0020719 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 5.5e-179
Identity = 320/610 (52.46%), Postives = 421/610 (69.02%), Query Frame = 0

Query: 7   RFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDI-DFTRSRDLVLPAGVVVP 66
           R     AAE A+  IG GYDL  DLRL  CK G   S+L+DI +   + ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 126
           NV  SI CDKGER RFRSD++ F QM+E FNQ+LSL+GKIPSG FN MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            T++LAFDG FI+LY++ L+KS + L E VK+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDA---NGGFTSNSVAAKIKDEYSLP 246
           GGKDVI+ +Q   S ++  D+QK+LK++AD +F E +   N G      ++K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVD-VGQSHNQWLSTISQSPNVISMSFV 306
             LR A  +S+     +++  ++   +  RRGG D     HN+WL T+   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQ 366
           PITSL+NGV G+GFLSHA+NLYLRYKPPIEEL QFLEFQLPRQWAPV+++LPL P+ ++Q
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQ 363

Query: 367 ASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLS 426
           +  SL+F+  GPKLYVNTT VD   RP+TG+RL+LEG++S+ LAIHLQHLS+LPK  QL 
Sbjct: 364 SCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLE 423

Query: 427 DDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIGM 486
           DDL+     E  ++ Y+E V W  +SHVCT PVE       DD  S+VT A   V+  G 
Sbjct: 424 DDLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHGF 483

Query: 487 RKVLFLRLGFSMVASAG-IRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 546
           + VLFLRL FS V  A  ++ S+W+     + KSGLIST IS  F+A    P     + +
Sbjct: 484 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 543

Query: 547 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 606
           NSA+YP GPP+ ++A K+L  +DT EM RGP+ SPGYWVV+GA+L VE G+IS+KVKYSL
Sbjct: 544 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 600

Query: 607 LTILSEDSMI 611
            T +  D +I
Sbjct: 604 FTPILGDEVI 600

BLAST of Pay0020719 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 2.4e-129
Identity = 254/615 (41.30%), Postives = 375/615 (60.98%), Query Frame = 0

Query: 15  EKAVSVIGFGYDLCNDLRLSSCKPGPSGS---KLIDIDFTRSRDLVLPAGVVVPNVPTSI 74
           E AV  +G G+DL  D RL  CK G   +   +L+ +D T++R+L +P   V  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 75  NCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLK-GCWQNDAGSTRSL 134
           NCDKGERTRFRSD++ FN+MSE FNQ+ S++GKIPSG FN  FG + G W  DA + +SL
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 135 AFDGCFITLYNIELEK-SHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKD 194
             D   +TL+N+ +   + + L+++V+  VPSSWDP  LA FIE+YGTHV+ GV +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 195 VIFMRQLRESNVEQTDVQKKLKKLADDKFSEDA-------NGGFTSNSVAAKIKDEYSLP 254
           V+ +RQ + S+++   ++  L  L D  F+          N  +  +    K  + +++ 
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNV- 249

Query: 255 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVP 314
               +    +     IN   G  +T I  +RGG    +SH++WL T+   P+ I+ +F+P
Sbjct: 250 --FDDKQTVAFNNFSINSQNG--ITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIP 309

Query: 315 ITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPL-APRHRKQ 374
           ITSL+  V G+G LSHA++LYLRYKPP+ +LQ FL+F  PR WAPV+ DLP  A  +   
Sbjct: 310 ITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMAS 369

Query: 375 ASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL--Q 434
           A P+L    MGPKLYVNTT V S   PVTG+R FLEGKK + LAIHLQHL     T+  +
Sbjct: 370 AYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEK 429

Query: 435 LSDDLSYRPIDE-PEEKGYFEPVKWSIFSHVCTAPVEYHDSLI--------DDDSASIVT 494
           ++D+  +R  D+  +   YFEP+    FSHVCT PV+Y  + I         +D A IVT
Sbjct: 430 ITDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVT 489

Query: 495 KAWFEVKVIGMRKVLFLRLGFSMVASAGIRRSDW-EGPSTTSRKSGLISTFISTRFSAGL 554
            A  EVK  G + VL LRL ++ V+   + ++ W  GP  TS+KSG+ S+      S  +
Sbjct: 490 GAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSV 549

Query: 555 HQ---PEEQPKIELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLC 602
           H     +++ ++ L+S V+P GPP+ +   K++  +D  ++ RGP++SPG+W+VTG +L 
Sbjct: 550 HHNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLY 609

BLAST of Pay0020719 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 5.4e-118
Identity = 238/600 (39.67%), Postives = 350/600 (58.33%), Query Frame = 0

Query: 17  AVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVPNVPTSINCDKG 76
           A+  +G G+D+ +D+RL  CK  P GS+L+ I+  ++RDL L  G ++PNVP  I+C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTRSLAFDGCF 136
                R  V SF++M+E FN +  + G IP G FN MF   G WQ DA ST+SLA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIFMRQL 196
           I LY+++L K  + L  +++R VPSSWDPA+LA FIE YGTH+V  V +GG+DV+++RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYSLPWELRNAFAASIKPP 256
           + S +  ++++  +  +   +F E       S S+   +K                    
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHE-----AESQSITGPLK-------------------- 263

Query: 257 VINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSLMNGVRGNGFL 316
                K  ++T I+ RRGG D+ QSH +W  T+  +P++I+M+F PI SL+ GV G   L
Sbjct: 264 ----YKDKDITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 323

Query: 317 SHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLY 376
           + A+ LYL YKPPIE+LQ FL++Q+ R WAP  ++L    + ++    SL+F+LMGPKL+
Sbjct: 324 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLF 383

Query: 377 VNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSYRPIDEPEEKG 436
           ++  +V    +PVTG+RL LEG K + L+IHLQHL +LPK LQ   D S+ PI  P+ +G
Sbjct: 384 ISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKWQG 443

Query: 437 -------YFEPVKWSIFSHVCTAPVEYHDSLIDD-DSASIVTKAWFEVKVIGMRKVLFLR 496
                  +FEP+KW  FSHV T+P+E+ ++ I D     IVT A   V   G + VL L+
Sbjct: 444 PEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLK 503

Query: 497 LGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYPDG 556
           L FS V    IRRS W+                        H P     +  +  + P G
Sbjct: 504 LLFSKVPGCTIRRSVWD------------------------HTP-----VASSGRLEPGG 559

Query: 557 PPLTS-------RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLL 602
           P  +S       ++ K+  ++D+ EM++GP++ PG+W+VTGAKL VE G+I ++VKYSLL
Sbjct: 564 PSTSSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of Pay0020719 vs. ExPASy TrEMBL
Match: A0A5D3DSF4 (MACPF domain-containing protein NSL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G001020 PE=4 SV=1)

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 609/610 (99.84%), Postives = 610/610 (100.00%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSV+GFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Pay0020719 vs. ExPASy TrEMBL
Match: A0A0A0LV66 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G075560 PE=4 SV=1)

HSP 1 Score: 1213.7 bits (3139), Expect = 0.0e+00
Identity = 603/610 (98.85%), Postives = 604/610 (99.02%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGG TSN VAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNN PVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEY+ SLIDDDSASIVTKAWFEVK IG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Pay0020719 vs. ExPASy TrEMBL
Match: A0A5A7UMD4 (MACPF domain-containing protein NSL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G001940 PE=4 SV=1)

HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 584/610 (95.74%), Postives = 591/610 (96.89%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSV+GFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIK    
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIK---- 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +P +  + + +   P   +H    ELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 VPTQ-EHKWLSPFGPFHWSH----ELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 601

BLAST of Pay0020719 vs. ExPASy TrEMBL
Match: A0A6J1JYX1 (MACPF domain-containing protein NSL1 OS=Cucurbita maxima OX=3661 GN=LOC111490102 PE=4 SV=1)

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 558/610 (91.48%), Postives = 587/610 (96.23%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFN+NRF+PQSAAEKAVSVIGFG+DLCNDLRLSSCKPGPSGSKLIDID TR++DLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV  SI CDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SHITLSEQVKREVPSSWDPAALA+FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQT+VQKKLK+LAD++FSEDANGGF SNS AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQTEVQKKLKQLADERFSEDANGGFMSNSAAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDEL  IY+RRGGVDVGQSHNQWLST+SQSPNVI MSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELMTIYVRRGGVDVGQSHNQWLSTVSQSPNVILMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSN+RPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480
           SDDLSY PIDEPEEKGYF+PVKWSIFSHVCTAPV+Y+ SLIDDD+ASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFS VASA IRRS+WEGPST+SRKSGLISTFISTRFS GL QPE QPKIEL
Sbjct: 481 MRKVLFLRLGFSTVASAMIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLT RAMKMLN+IDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTVRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Pay0020719 vs. ExPASy TrEMBL
Match: A0A6J1GVB1 (MACPF domain-containing protein NSL1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457211 PE=4 SV=1)

HSP 1 Score: 1137.9 bits (2942), Expect = 0.0e+00
Identity = 555/610 (90.98%), Postives = 586/610 (96.07%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFN+NRF+PQSAAEKAVSVIGFG+DLCNDLRLSSCKPGPSGSKLIDID TR++DLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV  SI CDKGERTRFRSDV+SFNQMSELFNQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVVSFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SHITLSEQVKREVPSSWDPAALA+FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQ +VQKKLK+LAD++FSED NGGF SNS AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDVNGGFMSNSTAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDELT IY+RRGGVDVGQSHNQWLST+SQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSL+NGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA R RK
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRLRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSN+RPVTGVRLFLEGKKSDHLAIHLQHLSTLPKT+QL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480
           SDDLSY PIDEPEEKGYF+PVKWSIFSHVCTAPV+Y+ SLIDDD+ASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFS VASA IRRS+WEGPST+SRKSGLISTFISTRFS GL QPE QPKIEL
Sbjct: 481 MRKVLFLRLGFSTVASAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLT RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTVRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Pay0020719 vs. NCBI nr
Match: TYK26438.1 (MACPF domain-containing protein NSL1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 609/610 (99.84%), Postives = 610/610 (100.00%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSV+GFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Pay0020719 vs. NCBI nr
Match: XP_004146657.1 (MACPF domain-containing protein NSL1 [Cucumis sativus] >KGN64702.1 hypothetical protein Csa_013635 [Cucumis sativus])

HSP 1 Score: 1213.7 bits (3139), Expect = 0.0e+00
Identity = 603/610 (98.85%), Postives = 604/610 (99.02%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGG TSN VAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNN PVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEY+ SLIDDDSASIVTKAWFEVK IG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Pay0020719 vs. NCBI nr
Match: XP_038894492.1 (MACPF domain-containing protein NSL1 [Benincasa hispida])

HSP 1 Score: 1206.8 bits (3121), Expect = 0.0e+00
Identity = 596/610 (97.70%), Postives = 604/610 (99.02%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRF+PQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTR+RDLVLP 
Sbjct: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRTRDLVLPG 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV TSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLAD+KFSEDANGGFTSN  AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDS+NRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSHNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480
           SDDLSYRPIDEPEEKGYFEPV+WSIFSHVCTAPVEY+ SLIDDDSASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVRWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFS+GLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSSGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLN+IDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Pay0020719 vs. NCBI nr
Match: KAA0057012.1 (MACPF domain-containing protein NSL1 [Cucumis melo var. makuwa])

HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 584/610 (95.74%), Postives = 591/610 (96.89%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSV+GFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIK    
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIK---- 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +P +  + + +   P   +H    ELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 VPTQ-EHKWLSPFGPFHWSH----ELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 601

BLAST of Pay0020719 vs. NCBI nr
Match: KAG6573287.1 (MACPF domain-containing protein NSL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1142.9 bits (2955), Expect = 0.0e+00
Identity = 557/610 (91.31%), Postives = 588/610 (96.39%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFN+NRF+PQSAAEKAVSVIGFG+DLCNDLRLSSCKPGPSGSKLIDID TR++DLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV  SI CDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SHITLSEQVKREVPSSWDPAAL++FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQ +VQKKLK+LAD++FSEDANGGF SNS AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDELT IY+RRGGVDVGQSHNQWLST+SQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSL+NGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RHRK
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSN+RPVTGVRLFLEGKKSDHLAIHLQHLSTLPKT+QL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480
           SDDLSY PIDEPEEKGYF+PVKWSIFSHVCTAPV+Y+ SLIDDD+ASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFS VASA IRRS+WEGPST+SRKSGLISTFISTRFS GL QPE QPKIEL
Sbjct: 481 MRKVLFLRLGFSTVASAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLT RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTVRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Pay0020719 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 783.9 bits (2023), Expect = 9.5e-227
Identity = 391/612 (63.89%), Postives = 475/612 (77.61%), Query Frame = 0

Query: 4   NINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVV 63
           N  R +  SAAEKAVSVIG GYDLC+D+R S+CK  P GS+L++ID TR+RDL+ P G+V
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQND 123
           V NV +SI CDKGERTR RSD++SFNQMSE FNQ + LSGKIPSG FN MF    CW  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGV 183
           A S ++LA+DG FI+LY++E+ +  +TL ++VKREVPSSWD AALA FIEKYGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSV-AAKIKDEYSLP 243
            MGGKDVI ++Q+R+SN E  ++QK LK   D++F  D     +  SV + K K+E  L 
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQ 244

Query: 244 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVP 303
           W L+  F  S+   V+ H+K +E+  + IRRGGVD+GQSH +WLST+SQ+PNVISM FVP
Sbjct: 245 WGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVP 304

Query: 304 ITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQA 363
           ITSL++G+ G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  R  KQ+
Sbjct: 305 ITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQS 364

Query: 364 SPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSD 423
           SPSL+F+LMGPKLYVNT+KVDS  RPVTG+R FLEGKK +HLAIHLQHLS  P +L LS 
Sbjct: 365 SPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSH 424

Query: 424 DLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIGMR 483
           D +Y PI+EP EKGY+ PVKW IFSHVCT PV+Y+ +   DD+ASIVTKAW EVK +GMR
Sbjct: 425 DDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKGMGMR 484

Query: 484 KVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLH----QPEEQPKI 543
           KVLFLRLGFS+ ASA  R+S W+  ST SRKSG+ S  ISTR S GL       + Q KI
Sbjct: 485 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKI 544

Query: 544 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 603
           ++NSAVYP GP    +  K+L+++DTKE++RGPE  PGYWVVTGAKLCVE G+ISIK KY
Sbjct: 545 DINSAVYPRGPSPPVKP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKY 604

Query: 604 SLLTILSEDSMI 611
           SLLT++SEDS++
Sbjct: 605 SLLTVISEDSLV 612

BLAST of Pay0020719 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 629.0 bits (1621), Expect = 3.9e-180
Identity = 320/610 (52.46%), Postives = 421/610 (69.02%), Query Frame = 0

Query: 7   RFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDI-DFTRSRDLVLPAGVVVP 66
           R     AAE A+  IG GYDL  DLRL  CK G   S+L+DI +   + ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 126
           NV  SI CDKGER RFRSD++ F QM+E FNQ+LSL+GKIPSG FN MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            T++LAFDG FI+LY++ L+KS + L E VK+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDA---NGGFTSNSVAAKIKDEYSLP 246
           GGKDVI+ +Q   S ++  D+QK+LK++AD +F E +   N G      ++K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVD-VGQSHNQWLSTISQSPNVISMSFV 306
             LR A  +S+     +++  ++   +  RRGG D     HN+WL T+   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQ 366
           PITSL+NGV G+GFLSHA+NLYLRYKPPIEEL QFLEFQLPRQWAPV+++LPL P+ ++Q
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQ 363

Query: 367 ASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLS 426
           +  SL+F+  GPKLYVNTT VD   RP+TG+RL+LEG++S+ LAIHLQHLS+LPK  QL 
Sbjct: 364 SCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLE 423

Query: 427 DDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIGM 486
           DDL+     E  ++ Y+E V W  +SHVCT PVE       DD  S+VT A   V+  G 
Sbjct: 424 DDLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHGF 483

Query: 487 RKVLFLRLGFSMVASAG-IRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 546
           + VLFLRL FS V  A  ++ S+W+     + KSGLIST IS  F+A    P     + +
Sbjct: 484 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 543

Query: 547 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 606
           NSA+YP GPP+ ++A K+L  +DT EM RGP+ SPGYWVV+GA+L VE G+IS+KVKYSL
Sbjct: 544 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 600

Query: 607 LTILSEDSMI 611
            T +  D +I
Sbjct: 604 FTPILGDEVI 600

BLAST of Pay0020719 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 464.2 bits (1193), Expect = 1.7e-130
Identity = 254/615 (41.30%), Postives = 375/615 (60.98%), Query Frame = 0

Query: 15  EKAVSVIGFGYDLCNDLRLSSCKPGPSGS---KLIDIDFTRSRDLVLPAGVVVPNVPTSI 74
           E AV  +G G+DL  D RL  CK G   +   +L+ +D T++R+L +P   V  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 75  NCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLK-GCWQNDAGSTRSL 134
           NCDKGERTRFRSD++ FN+MSE FNQ+ S++GKIPSG FN  FG + G W  DA + +SL
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 135 AFDGCFITLYNIELEK-SHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKD 194
             D   +TL+N+ +   + + L+++V+  VPSSWDP  LA FIE+YGTHV+ GV +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 195 VIFMRQLRESNVEQTDVQKKLKKLADDKFSEDA-------NGGFTSNSVAAKIKDEYSLP 254
           V+ +RQ + S+++   ++  L  L D  F+          N  +  +    K  + +++ 
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNV- 249

Query: 255 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVP 314
               +    +     IN   G  +T I  +RGG    +SH++WL T+   P+ I+ +F+P
Sbjct: 250 --FDDKQTVAFNNFSINSQNG--ITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIP 309

Query: 315 ITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPL-APRHRKQ 374
           ITSL+  V G+G LSHA++LYLRYKPP+ +LQ FL+F  PR WAPV+ DLP  A  +   
Sbjct: 310 ITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMAS 369

Query: 375 ASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL--Q 434
           A P+L    MGPKLYVNTT V S   PVTG+R FLEGKK + LAIHLQHL     T+  +
Sbjct: 370 AYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEK 429

Query: 435 LSDDLSYRPIDE-PEEKGYFEPVKWSIFSHVCTAPVEYHDSLI--------DDDSASIVT 494
           ++D+  +R  D+  +   YFEP+    FSHVCT PV+Y  + I         +D A IVT
Sbjct: 430 ITDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVT 489

Query: 495 KAWFEVKVIGMRKVLFLRLGFSMVASAGIRRSDW-EGPSTTSRKSGLISTFISTRFSAGL 554
            A  EVK  G + VL LRL ++ V+   + ++ W  GP  TS+KSG+ S+      S  +
Sbjct: 490 GAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSV 549

Query: 555 HQ---PEEQPKIELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLC 602
           H     +++ ++ L+S V+P GPP+ +   K++  +D  ++ RGP++SPG+W+VTG +L 
Sbjct: 550 HHNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLY 609

BLAST of Pay0020719 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 426.4 bits (1095), Expect = 3.9e-119
Identity = 238/600 (39.67%), Postives = 350/600 (58.33%), Query Frame = 0

Query: 17  AVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVPNVPTSINCDKG 76
           A+  +G G+D+ +D+RL  CK  P GS+L+ I+  ++RDL L  G ++PNVP  I+C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTRSLAFDGCF 136
                R  V SF++M+E FN +  + G IP G FN MF   G WQ DA ST+SLA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIFMRQL 196
           I LY+++L K  + L  +++R VPSSWDPA+LA FIE YGTH+V  V +GG+DV+++RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYSLPWELRNAFAASIKPP 256
           + S +  ++++  +  +   +F E       S S+   +K                    
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHE-----AESQSITGPLK-------------------- 263

Query: 257 VINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSLMNGVRGNGFL 316
                K  ++T I+ RRGG D+ QSH +W  T+  +P++I+M+F PI SL+ GV G   L
Sbjct: 264 ----YKDKDITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 323

Query: 317 SHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLY 376
           + A+ LYL YKPPIE+LQ FL++Q+ R WAP  ++L    + ++    SL+F+LMGPKL+
Sbjct: 324 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLF 383

Query: 377 VNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSYRPIDEPEEKG 436
           ++  +V    +PVTG+RL LEG K + L+IHLQHL +LPK LQ   D S+ PI  P+ +G
Sbjct: 384 ISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKWQG 443

Query: 437 -------YFEPVKWSIFSHVCTAPVEYHDSLIDD-DSASIVTKAWFEVKVIGMRKVLFLR 496
                  +FEP+KW  FSHV T+P+E+ ++ I D     IVT A   V   G + VL L+
Sbjct: 444 PEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLK 503

Query: 497 LGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYPDG 556
           L FS V    IRRS W+                        H P     +  +  + P G
Sbjct: 504 LLFSKVPGCTIRRSVWD------------------------HTP-----VASSGRLEPGG 559

Query: 557 PPLTS-------RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLL 602
           P  +S       ++ K+  ++D+ EM++GP++ PG+W+VTGAKL VE G+I ++VKYSLL
Sbjct: 564 PSTSSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of Pay0020719 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 325.1 bits (832), Expect = 1.2e-88
Identity = 167/327 (51.07%), Postives = 223/327 (68.20%), Query Frame = 0

Query: 7   RFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDI-DFTRSRDLVLPAGVVVP 66
           R     AAE A+  IG GYDL  DLRL  CK G   S+L+DI +   + ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 126
           NV  SI CDKGER RFRSD++ F QM+E FNQ+LSL+GKIPSG FN MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            T++LAFDG FI+LY++ L+KS + L E VK+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDA---NGGFTSNSVAAKIKDEYSLP 246
           GGKDVI+ +Q   S ++  D+QK+LK++AD +F E +   N G      ++K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVD-VGQSHNQWLSTISQSPNVISMSFV 306
             LR A  +S+     +++  ++   +  RRGG D     HN+WL T+   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLMNGVRGNGFLSHAVNLYLRYKP 329
           PITSL+NGV G+GFLSHA+NLYLR KP
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRCKP 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SGN61.3e-22563.89MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=... [more]
Q9STW55.5e-17952.46MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4... [more]
Q8L6122.4e-12941.30MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Q9C7N25.4e-11839.67MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=... [more]
Match NameE-valueIdentityDescription
A0A5D3DSF40.0e+0099.84MACPF domain-containing protein NSL1-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A0A0LV660.0e+0098.85MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G075560 PE=4 ... [more]
A0A5A7UMD40.0e+0095.74MACPF domain-containing protein NSL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1JYX10.0e+0091.48MACPF domain-containing protein NSL1 OS=Cucurbita maxima OX=3661 GN=LOC111490102... [more]
A0A6J1GVB10.0e+0090.98MACPF domain-containing protein NSL1 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
TYK26438.10.0e+0099.84MACPF domain-containing protein NSL1-like [Cucumis melo var. makuwa][more]
XP_004146657.10.0e+0098.85MACPF domain-containing protein NSL1 [Cucumis sativus] >KGN64702.1 hypothetical ... [more]
XP_038894492.10.0e+0097.70MACPF domain-containing protein NSL1 [Benincasa hispida][more]
KAA0057012.10.0e+0095.74MACPF domain-containing protein NSL1 [Cucumis melo var. makuwa][more]
KAG6573287.10.0e+0091.31MACPF domain-containing protein NSL1, partial [Cucurbita argyrosperma subsp. sor... [more]
Match NameE-valueIdentityDescription
AT1G28380.19.5e-22763.89MAC/Perforin domain-containing protein [more]
AT4G24290.23.9e-18052.46MAC/Perforin domain-containing protein [more]
AT1G14780.11.7e-13041.30MAC/Perforin domain-containing protein [more]
AT1G29690.13.9e-11939.67MAC/Perforin domain-containing protein [more]
AT4G24290.11.2e-8851.07MAC/Perforin domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020864Membrane attack complex component/perforin (MACPF) domainSMARTSM00457MACPF_8coord: 127..325
e-value: 3.6E-11
score: 53.1
IPR020864Membrane attack complex component/perforin (MACPF) domainPFAMPF01823MACPFcoord: 110..323
e-value: 4.2E-34
score: 118.5
IPR020864Membrane attack complex component/perforin (MACPF) domainPROSITEPS51412MACPF_2coord: 2..337
score: 23.419956
NoneNo IPR availablePANTHERPTHR33199:SF18BNAA09G27290D PROTEINcoord: 1..609
IPR044663MACPF domain-containing protein CAD1/NSL1-likePANTHERPTHR33199MACPF DOMAIN-CONTAINING PROTEIN CAD1coord: 1..609

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0020719.1Pay0020719.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0052542 defense response by callose deposition
biological_process GO:0009626 plant-type hypersensitive response
biological_process GO:2000031 regulation of salicylic acid mediated signaling pathway
biological_process GO:0010337 regulation of salicylic acid metabolic process
biological_process GO:0006952 defense response
biological_process GO:0012501 programmed cell death