Pay0020265 (gene) Melon (Payzawat) v1

Overview


Name	Pay0020265
Type	gene
Organism	Cucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description	DNA oxidative demethylase ALKBH2
Location	chr08: 4389628 .. 4395290 (-)
RNA-Seq Expression	Pay0020265
Synteny	Pay0020265

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGACATTTTATGGACATTAAACCTCTGTTCGTGGCCTTCCGGGCGCAGTTACCGGTGGCATCGATCAAATTAAATTTGGCGCAAAAGAAACGAAACGAAACGAGAGCACGAACTATATAAATATGGTACAGTTAAGAAGACAAGAGCCGCAAATTATGAAGAAATAATGAGTCTCAGGTTCAAAGAAGCGACCGACTCAGAAACGGAGACGAAGATCCCTCGTCCTTTTGATCTAGGAAAGCGGCAGACGTTGGATCTCGGTAATGGAAGCGAAATCCTCTACATTAGCAAGTTCGTCTCCTCCGATCAGGCTTGGACCTGGTTCGATTATCTCAATCAACGTATTCCATGGACCAGGCCCACCATTCGCGTCTTCGGTCGCTCCGTTCTTCAGGTCTTCCCCTTTCTCCATTTCTTATGTTAGGGTTTTGAGTTATTGCTGAACAGCTATAGCATTGTTCGGCGGTTTTTAAGTTTTAATTTGCTAATGCAGCCTAGAGATACTTGCTACGTTGCACATCCTGGATTGACTACATTAACTTATAGTGGGTATAAACCTCATGCCTATACTTGGGATGATTTTACTCCTCTTAAGGACATTTTGGATGCTGTAAGATTTTTTGTTTCGATCCTTCCGTATTGGGTTTTCTTTAGCTACATGTTCTTGTTGACGTTTCGAGTGGCTCCTGATTGGCTTCACTTTTTTCTCTGTCAAGGTCCACGAAGCTCTTCCGGGAAGCAGATTTAATAGCTTACTCTTGAACAGGTATAAAGGTGGTAATGATTATGTGGGTTGGCATTCTGATGATGAGAAAGTTTATGCACTGAACCAGGAGATTGCTTCTGTATCATTTGGATGTGAACGGGATTTCTTGTTGAAGAAGAAGCCCAACAAAACTTCTCAACGTATCCAGTCTACTTTATCTGTTTCTTTTTTGTTTCTCTGTTCTGATTTGGAAAGCCCTTTTCTCAAATCTTCAGTCTTTTGCTCAATTGCTTTCTAGTTCCATTATCTAATGGTTTATTTGACGTGGCCACAGGAAGAAATGATGAGGAGCCTCCAGCCAAGAAATCAAAGAAATGCAGTGGGGCTGATACACACTCATTTGTACTAAAGCATGGTTCACTTTTGGTTATGAAAGGCTACACTCAACGAGATTGGATGCATTCAGTGCCTAAGCGCCTTAAGGCAGAGGCAACCCGCATCAATCTTACTTTCAGACACATTATAGGTGATTGATTTTGAAGTTAATACCAGATTGTTGTAGGAATGTCCTTCGTAAGGTTCAAGTGGCTCTATCTTTGGAGTGGACGTTTCATTAGTTTTGGTATGTATTTTTCTTGTCTATTGTGTACATTTCTTTCATAGAGCAATGTAAGGTGGAGTAGATCCGGTTTCTCAATTGGCTCATCTATTATATTCGCTTCCCTGTCTCTTATTGCATTTCAAATTCTTTATGATTGCTCATTTTCATCTACAGCTCGCAGCTACCCGGAATCAAAT

mRNA sequence

TGACATTTTATGGACATTAAACCTCTGTTCGTGGCCTTCCGGGCGCAGTTACCGGTGGCATCGATCAAATTAAATTTGGCGCAAAAGAAACGAAACGAAACGAGAGCACGAACTATATAAATATGGTACAGTTAAGAAGACAAGAGCCGCAAATTATGAAGAAATAATGAGTCTCAGGTTCAAAGAAGCGACCGACTCAGAAACGGAGACGAAGATCCCTCGTCCTTTTGATCTAGGAAAGCGGCAGACGTTGGATCTCGGTAATGGAAGCGAAATCCTCTACATTAGCAAGTTCGTCTCCTCCGATCAGGCTTGGACCTGGTTCGATTATCTCAATCAACGTATTCCATGGACCAGGCCCACCATTCGCGTCTTCGGTCGCTCCGTTCTTCAGCCTAGAGATACTTGCTACGTTGCACATCCTGGATTGACTACATTAACTTATAGTGGGTATAAACCTCATGCCTATACTTGGGATGATTTTACTCCTCTTAAGGACATTTTGGATGCTGTCCACGAAGCTCTTCCGGGAAGCAGATTTAATAGCTTACTCTTGAACAGGTATAAAGGTGGTAATGATTATGTGGGTTGGCATTCTGATGATGAGAAAGTTTATGCACTGAACCAGGAGATTGCTTCTGTATCATTTGGATGTGAACGGGATTTCTTGTTGAAGAAGAAGCCCAACAAAACTTCTCAACGAAGAAATGATGAGGAGCCTCCAGCCAAGAAATCAAAGAAATGCAGTGGGGCTGATACACACTCATTTGTACTAAAGCATGGTTCACTTTTGGTTATGAAAGGCTACACTCAACGAGATTGGATGCATTCAGTGCCTAAGCGCCTTAAGGCAGAGGCAACCCGCATCAATCTTACTTTCAGACACATTATAGGTGATTGATTTTGAAGTTAATACCAGATTGTTGTAGGAATGTCCTTCGTAAGGTTCAAGTGGCTCTATCTTTGGAGTGGACGTTTCATTAGTTTTGGTATGTATTTTTCTTGTCTATTGTGTACATTTCTTTCATAGAGCAATGTAAGGTGGAGTAGATCCGGTTTCTCAATTGGCTCATCTATTATATTCGCTTCCCTGTCTCTTATTGCATTTCAAATTCTTTATGATTGCTCATTTTCATCTACAGCTCGCAGCTACCCGGAATCAAAT

Coding sequence (CDS)

ATGAGTCTCAGGTTCAAAGAAGCGACCGACTCAGAAACGGAGACGAAGATCCCTCGTCCTTTTGATCTAGGAAAGCGGCAGACGTTGGATCTCGGTAATGGAAGCGAAATCCTCTACATTAGCAAGTTCGTCTCCTCCGATCAGGCTTGGACCTGGTTCGATTATCTCAATCAACGTATTCCATGGACCAGGCCCACCATTCGCGTCTTCGGTCGCTCCGTTCTTCAGCCTAGAGATACTTGCTACGTTGCACATCCTGGATTGACTACATTAACTTATAGTGGGTATAAACCTCATGCCTATACTTGGGATGATTTTACTCCTCTTAAGGACATTTTGGATGCTGTCCACGAAGCTCTTCCGGGAAGCAGATTTAATAGCTTACTCTTGAACAGGTATAAAGGTGGTAATGATTATGTGGGTTGGCATTCTGATGATGAGAAAGTTTATGCACTGAACCAGGAGATTGCTTCTGTATCATTTGGATGTGAACGGGATTTCTTGTTGAAGAAGAAGCCCAACAAAACTTCTCAACGAAGAAATGATGAGGAGCCTCCAGCCAAGAAATCAAAGAAATGCAGTGGGGCTGATACACACTCATTTGTACTAAAGCATGGTTCACTTTTGGTTATGAAAGGCTACACTCAACGAGATTGGATGCATTCAGTGCCTAAGCGCCTTAAGGCAGAGGCAACCCGCATCAATCTTACTTTCAGACACATTATAGGTGATTGA

Protein sequence

MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRIPWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEALPGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRRNDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHIIGD

Homology

[Close]

BLAST of Pay0020265 vs. ExPASy Swiss-Prot
Match: Q9SIE0 (DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 SV=2)

HSP 1 Score: 321.6 bits (823), Expect = 7.5e-87
Identity = 152/244 (62.30%), Postives = 187/244 (76.64%), Query Frame = 0


Query: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60
           MSL    A +S + +  P       R+T+DLG+GS+++YI +F+   Q+WT+FDYL++ I
Sbjct: 77  MSLTSIVAVESPSSSNAP------SRKTIDLGHGSDLIYIQRFLPFQQSWTFFDYLDKHI 136

Query: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120
           PWTRPTIRVFGRS LQPRDTCYVA  GLT L YSGY+P +Y+WDDF PLK+ILDA+++ L
Sbjct: 137 PWTRPTIRVFGRSCLQPRDTCYVASSGLTALVYSGYRPTSYSWDDFPPLKEILDAIYKVL 196

Query: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQ-- 180
           PGSRFNSLLLNRYKG +DYV WH+DDEK+Y    EIASVSFGCERDF+LKKK ++ S   
Sbjct: 197 PGSRFNSLLLNRYKGASDYVAWHADDEKIYGPTPEIASVSFGCERDFVLKKKKDEESSQG 256

Query: 181 RRNDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTF 240
           +  D  P  K+ K+ S  D  S  LKHGSLLVM+GYTQRDW+HSVPKR KAE TRINLTF
Sbjct: 257 KTGDSGPAKKRLKRSSREDQQSLTLKHGSLLVMRGYTQRDWIHSVPKRAKAEGTRINLTF 314

Query: 241 RHII 243
           R ++
Sbjct: 317 RLVL 314

[Close]

BLAST of Pay0020265 vs. ExPASy Swiss-Prot
Match: Q58DM4 (DNA oxidative demethylase ALKBH2 OS=Bos taurus OX=9913 GN=ALKBH2 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.9e-21
Identity = 66/176 (37.50%), Postives = 90/176 (51.14%), Query Frame = 0


Query: 67  IRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEALPGSRFN 126
           ++VFG+    PR        GL T T+SG       W     L+ + D V   + G  FN
Sbjct: 98  VQVFGKWHSVPRKQATYGDTGL-TYTFSGLTLSPKPW--IPVLERVRDRV-SLVTGQTFN 157

Query: 127 SLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRRNDEEPP 186
            +L+NRYK G D++G H DDE+  AL   IASVSFG  RDF+ + K ++          P
Sbjct: 158 FVLINRYKDGQDHIGEHRDDERELALGSPIASVSFGACRDFVFRHKDSRGKH-------P 217

Query: 187 AKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHII 243
           +++       +     L HGSLL+M   T   W HS+P R K  A R+NLTFR I+
Sbjct: 218 SRR------LEVVRLQLAHGSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRKIL 256

[Close]

BLAST of Pay0020265 vs. ExPASy Swiss-Prot
Match: Q32L00 (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Bos taurus OX=9913 GN=ALKBH3 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 4.2e-21
Identity = 73/214 (34.11%), Postives = 98/214 (45.79%), Query Frame = 0


Query: 35  SEILYISKFVSSDQAWTWFDYLNQRIPWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYS 94
           S +     FV   +A + F+ L + +PW + T      +  QPR T +    G    TYS
Sbjct: 88  SRVCLCPGFVDLKEADSVFEQLCRDVPWKQRTGIRDDVTYQQPRLTAWY---GELPYTYS 147

Query: 95  GY----KPHAYTWDDFTPLKDILDAVHEALPGSRFNSLLLNRYKGGNDYVGWHSDDEKVY 154
                  PH      + P+  +L    E   G  FNSLL N Y+   D V WHSDDE   
Sbjct: 148 RITMEPNPH------WHPVLLMLKNQIEENTGHSFNSLLCNLYRNEKDSVDWHSDDEPSL 207

Query: 155 ALNQEIASVSFGCERDFLLKKKPNKTSQRRNDEEPPAKKSKKCSGADTHSFVLKHGSLLV 214
                IAS+SFG  R F ++KK            PP + +   +  +     L HG+LL+
Sbjct: 208 GRCPIIASLSFGATRMFEMRKK------------PPPEDNGDYTYVERVKIPLDHGTLLI 267

Query: 215 MKGYTQRDWMHSVPKRLKAEATRINLTFRHIIGD 245
           M+G TQ DW H VPK   +   RINLTFR +  D
Sbjct: 268 MEGATQADWQHRVPKEYHSREPRINLTFRTVYPD 280

[Close]

BLAST of Pay0020265 vs. ExPASy Swiss-Prot
Match: Q96Q83 (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Homo sapiens OX=9606 GN=ALKBH3 PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 9.4e-21
Identity = 71/212 (33.49%), Postives = 98/212 (46.23%), Query Frame = 0


Query: 35  SEILYISKFVSSDQAWTWFDYLNQRIPWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYS 94
           S +     FV   +A    + L Q +PW + T      +  QPR T +    G    TYS
Sbjct: 88  SRVCLYPGFVDVKEADWILEQLCQDVPWKQRTGIREDITYQQPRLTAWY---GELPYTYS 147

Query: 95  --GYKPHAYTWDDFTPLKDILDAVHEALPGSRFNSLLLNRYKGGNDYVGWHSDDEKVYAL 154
               +P+ +       LK+ +    E   G  FNSLL N Y+   D V WHSDDE     
Sbjct: 148 RITMEPNPHWHPVLRTLKNRI----EENTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGR 207

Query: 155 NQEIASVSFGCERDFLLKKKPNKTSQRRNDEEPPAKKSKKCSGADTHSFVLKHGSLLVMK 214
              IAS+SFG  R F ++KK            PP +++   +  +     L HG+LL+M+
Sbjct: 208 CPIIASLSFGATRTFEMRKK------------PPPEENGDYTYVERVKIPLDHGTLLIME 267

Query: 215 GYTQRDWMHSVPKRLKAEATRINLTFRHIIGD 245
           G TQ DW H VPK   +   R+NLTFR +  D
Sbjct: 268 GATQADWQHRVPKEYHSREPRVNLTFRTVYPD 280

[Close]

BLAST of Pay0020265 vs. ExPASy Swiss-Prot
Match: Q6P6J4 (DNA oxidative demethylase ALKBH2 OS=Mus musculus OX=10090 GN=Alkbh2 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 3.6e-20
Identity = 66/176 (37.50%), Postives = 88/176 (50.00%), Query Frame = 0


Query: 67  IRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEALPGSRFN 126
           ++VFG+    PR        GL T T+SG       W     L+ + D V E + G  FN
Sbjct: 77  VQVFGKWHSVPRKQATYGDAGL-TYTFSGLTLTPKPW--VPVLERVRDRVCE-VTGQTFN 136

Query: 127 SLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRRNDEEPP 186
            +L+NRYK G D++G H DDE+  A    IASVSFG  RDF+ + K ++  + R   E  
Sbjct: 137 FVLVNRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFIFRHKDSRGKRPRRTVE-- 196

Query: 187 AKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHII 243
                           L HGSLL+M   T   W HS+P R +  A R+NLTFR I+
Sbjct: 197 -----------VVRLQLAHGSLLMMNPPTNTHWYHSLPIRKRVLAPRVNLTFRKIL 235

[Close]

BLAST of Pay0020265 vs. ExPASy TrEMBL
Match: A0A5A7SMW2 (DNA oxidative demethylase ALKBH2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold253G00710 PE=4 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 1.9e-141
Identity = 242/244 (99.18%), Postives = 243/244 (99.59%), Query Frame = 0


Query: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60
           MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI
Sbjct: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60

Query: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120
           PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL
Sbjct: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180
           PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDE PPAKKSKKC+GADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEVPPAKKSKKCNGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 IIGD 245
           IIGD
Sbjct: 241 IIGD 244

[Close]

BLAST of Pay0020265 vs. ExPASy TrEMBL
Match: A0A5D3CPV3 (DNA oxidative demethylase ALKBH2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00320 PE=4 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.2e-140
Identity = 241/244 (98.77%), Postives = 241/244 (98.77%), Query Frame = 0


Query: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60
           MSLRFKE TDSETETKIPRPFD GKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI
Sbjct: 1   MSLRFKEVTDSETETKIPRPFDPGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60

Query: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120
           PWTRPTIRVFGRSVLQPRDTCYVAHPG TTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL
Sbjct: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGSTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180
           PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 IIGD 245
           IIGD
Sbjct: 241 IIGD 244

[Close]

BLAST of Pay0020265 vs. ExPASy TrEMBL
Match: A0A1S3B2C3 (DNA oxidative demethylase ALKBH2 OS=Cucumis melo OX=3656 GN=LOC103485223 PE=4 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 2.7e-140
Identity = 240/244 (98.36%), Postives = 241/244 (98.77%), Query Frame = 0


Query: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60
           MSLRFKE TDSETETKIPRPFD GKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI
Sbjct: 1   MSLRFKEVTDSETETKIPRPFDPGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60

Query: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120
           PWTRPTIRVFGRSVLQPRDTCYVAHPG TTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL
Sbjct: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGSTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180
           PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYA+NQEIASVSFGCERDFLLKKKPNKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYAVNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 IIGD 245
           IIGD
Sbjct: 241 IIGD 244

[Close]

BLAST of Pay0020265 vs. ExPASy TrEMBL
Match: A0A0A0KL20 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G510990 PE=4 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.0e-135
Identity = 234/244 (95.90%), Postives = 238/244 (97.54%), Query Frame = 0


Query: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60
           MSLRFKE TDSE+ETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFD+LNQRI
Sbjct: 1   MSLRFKEVTDSESETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDFLNQRI 60

Query: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120
           PWTRPTI VFGRSVLQPRDTCYVA+PGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL
Sbjct: 61  PWTRPTICVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180
           PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKK S AD HSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADRHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 IIGD 245
           +I D
Sbjct: 241 VIVD 244

[Close]

BLAST of Pay0020265 vs. ExPASy TrEMBL
Match: A0A6J1GEX9 (DNA oxidative demethylase ALKBH2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453571 PE=4 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 2.4e-128
Identity = 220/241 (91.29%), Postives = 230/241 (95.44%), Query Frame = 0


Query: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60
           MSLRFKE  DSE+ETKIPRP DLG+RQTLDLGNGSEI+YISKFVSSDQAWTWFD+LNQ I
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120
           PWTRPTIRVFGRSV+QPRDTCYV +PGLTT +YSGYKPHAYTWDDF PLKDIL+AVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180
           PGS FNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVSFGCERDFLLKKK NKTSQRR
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 194

Query: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKK S ADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 195 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 254

Query: 241 I 242
           +
Sbjct: 255 V 255

[Close]

BLAST of Pay0020265 vs. NCBI nr
Match: KAA0025581.1 (DNA oxidative demethylase ALKBH2 [Cucumis melo var. makuwa])

HSP 1 Score: 511.5 bits (1316), Expect = 3.9e-141
Identity = 242/244 (99.18%), Postives = 243/244 (99.59%), Query Frame = 0


Query: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60
           MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI
Sbjct: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60

Query: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120
           PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL
Sbjct: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180
           PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDE PPAKKSKKC+GADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEVPPAKKSKKCNGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 IIGD 245
           IIGD
Sbjct: 241 IIGD 244

[Close]

BLAST of Pay0020265 vs. NCBI nr
Match: TYK12456.1 (DNA oxidative demethylase ALKBH2 [Cucumis melo var. makuwa])

HSP 1 Score: 508.8 bits (1309), Expect = 2.5e-140
Identity = 241/244 (98.77%), Postives = 241/244 (98.77%), Query Frame = 0


Query: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60
           MSLRFKE TDSETETKIPRPFD GKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI
Sbjct: 1   MSLRFKEVTDSETETKIPRPFDPGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60

Query: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120
           PWTRPTIRVFGRSVLQPRDTCYVAHPG TTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL
Sbjct: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGSTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180
           PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 IIGD 245
           IIGD
Sbjct: 241 IIGD 244

[Close]

BLAST of Pay0020265 vs. NCBI nr
Match: XP_008440972.1 (PREDICTED: DNA oxidative demethylase ALKBH2 [Cucumis melo] >XP_016899335.1 PREDICTED: DNA oxidative demethylase ALKBH2 [Cucumis melo] >XP_016899336.1 PREDICTED: DNA oxidative demethylase ALKBH2 [Cucumis melo])

HSP 1 Score: 507.7 bits (1306), Expect = 5.7e-140
Identity = 240/244 (98.36%), Postives = 241/244 (98.77%), Query Frame = 0


Query: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60
           MSLRFKE TDSETETKIPRPFD GKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI
Sbjct: 1   MSLRFKEVTDSETETKIPRPFDPGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60

Query: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120
           PWTRPTIRVFGRSVLQPRDTCYVAHPG TTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL
Sbjct: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGSTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180
           PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYA+NQEIASVSFGCERDFLLKKKPNKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYAVNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 IIGD 245
           IIGD
Sbjct: 241 IIGD 244

[Close]

BLAST of Pay0020265 vs. NCBI nr
Match: XP_004134811.1 (DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742939.1 DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742940.1 DNA oxidative demethylase ALKBH2 [Cucumis sativus] >KGN49032.1 hypothetical protein Csa_003044 [Cucumis sativus])

HSP 1 Score: 491.5 bits (1264), Expect = 4.2e-135
Identity = 234/244 (95.90%), Postives = 238/244 (97.54%), Query Frame = 0


Query: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60
           MSLRFKE TDSE+ETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFD+LNQRI
Sbjct: 1   MSLRFKEVTDSESETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDFLNQRI 60

Query: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120
           PWTRPTI VFGRSVLQPRDTCYVA+PGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL
Sbjct: 61  PWTRPTICVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180
           PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKK S AD HSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADRHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 IIGD 245
           +I D
Sbjct: 241 VIVD 244

[Close]

BLAST of Pay0020265 vs. NCBI nr
Match: XP_038882915.1 (DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882916.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882917.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882918.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882919.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882920.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882921.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida])

HSP 1 Score: 476.5 bits (1225), Expect = 1.4e-130
Identity = 224/244 (91.80%), Postives = 234/244 (95.90%), Query Frame = 0


Query: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60
           MSLR KE TDSE+ETKIPRPF+ G+RQTLDLGNGSEI+Y+ KFV SDQAWTWFD+LNQRI
Sbjct: 1   MSLRLKEVTDSESETKIPRPFNSGRRQTLDLGNGSEIVYVPKFVPSDQAWTWFDFLNQRI 60

Query: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120
           PWTRPTIRVFGRSVLQPRDTCYVA+PGLTTLTYSGYKPHAYTWDDF PLKDILDAVHEAL
Sbjct: 61  PWTRPTIRVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFPPLKDILDAVHEAL 120

Query: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180
           PGS FNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR
Sbjct: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPAKKSKK S AD+HSFVLKHGSLLVM+GYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADSHSFVLKHGSLLVMRGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 IIGD 245
           +I D
Sbjct: 241 VIVD 244

[Close]

BLAST of Pay0020265 vs. TAIR 10
Match: AT2G22260.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 321.6 bits (823), Expect = 5.4e-88
Identity = 152/244 (62.30%), Postives = 187/244 (76.64%), Query Frame = 0


Query: 1   MSLRFKEATDSETETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDYLNQRI 60
           MSL    A +S + +  P       R+T+DLG+GS+++YI +F+   Q+WT+FDYL++ I
Sbjct: 77  MSLTSIVAVESPSSSNAP------SRKTIDLGHGSDLIYIQRFLPFQQSWTFFDYLDKHI 136

Query: 61  PWTRPTIRVFGRSVLQPRDTCYVAHPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120
           PWTRPTIRVFGRS LQPRDTCYVA  GLT L YSGY+P +Y+WDDF PLK+ILDA+++ L
Sbjct: 137 PWTRPTIRVFGRSCLQPRDTCYVASSGLTALVYSGYRPTSYSWDDFPPLKEILDAIYKVL 196

Query: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQ-- 180
           PGSRFNSLLLNRYKG +DYV WH+DDEK+Y    EIASVSFGCERDF+LKKK ++ S   
Sbjct: 197 PGSRFNSLLLNRYKGASDYVAWHADDEKIYGPTPEIASVSFGCERDFVLKKKKDEESSQG 256

Query: 181 RRNDEEPPAKKSKKCSGADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTF 240
           +  D  P  K+ K+ S  D  S  LKHGSLLVM+GYTQRDW+HSVPKR KAE TRINLTF
Sbjct: 257 KTGDSGPAKKRLKRSSREDQQSLTLKHGSLLVMRGYTQRDWIHSVPKRAKAEGTRINLTF 314

Query: 241 RHII 243
           R ++
Sbjct: 317 RLVL 314

The following BLAST results are available for this feature:

BLAST of Pay0020265 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIE0	7.5e-87	62.30	DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 ...	[more]
Q58DM4	1.9e-21	37.50	DNA oxidative demethylase ALKBH2 OS=Bos taurus OX=9913 GN=ALKBH2 PE=2 SV=1	[more]
Q32L00	4.2e-21	34.11	Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Bos taurus OX=9913 G...	[more]
Q96Q83	9.4e-21	33.49	Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Homo sapiens OX=9606...	[more]
Q6P6J4	3.6e-20	37.50	DNA oxidative demethylase ALKBH2 OS=Mus musculus OX=10090 GN=Alkbh2 PE=1 SV=1	[more]

BLAST of Pay0020265 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SMW2	1.9e-141	99.18	DNA oxidative demethylase ALKBH2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5D3CPV3	1.2e-140	98.77	DNA oxidative demethylase ALKBH2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B2C3	2.7e-140	98.36	DNA oxidative demethylase ALKBH2 OS=Cucumis melo OX=3656 GN=LOC103485223 PE=4 SV...	[more]
A0A0A0KL20	2.0e-135	95.90	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A6J1GEX9	2.4e-128	91.29	DNA oxidative demethylase ALKBH2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

BLAST of Pay0020265 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0025581.1	3.9e-141	99.18	DNA oxidative demethylase ALKBH2 [Cucumis melo var. makuwa]	[more]
TYK12456.1	2.5e-140	98.77	DNA oxidative demethylase ALKBH2 [Cucumis melo var. makuwa]	[more]
XP_008440972.1	5.7e-140	98.36	PREDICTED: DNA oxidative demethylase ALKBH2 [Cucumis melo] >XP_016899335.1 PREDI...	[more]
XP_004134811.1	4.2e-135	95.90	DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742939.1 DNA oxidative...	[more]
XP_038882915.1	1.4e-130	91.80	DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882916.1 DNA oxidati...	[more]

BLAST of Pay0020265 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22260.1	5.4e-88	62.30	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 38..239 e-value: 3.1E-32 score: 112.3
IPR037151	Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily	GENE3D	2.60.120.590		coord: 25..243 e-value: 2.8E-60 score: 205.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 172..194
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 33..241
IPR032852	DNA oxidative demethylase ALKBH2	PANTHER	PTHR31573	ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 2	coord: 23..241
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 124..242 score: 12.920472

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Pay0020265.2	Pay0020265.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006307	DNA dealkylation involved in DNA repair
biological_process	GO:0032259	methylation
biological_process	GO:0035511	oxidative DNA demethylation
biological_process	GO:0006281	DNA repair
molecular_function	GO:0051747	cytosine C-5 DNA demethylase activity
molecular_function	GO:0043734	DNA-N1-methyladenine dioxygenase activity
molecular_function	GO:0008198	ferrous iron binding
molecular_function	GO:0008168	methyltransferase activity