Pay0019811 (gene) Melon (Payzawat) v1

Overview
NamePay0019811
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationchr01: 34484216 .. 34485510 (+)
RNA-Seq ExpressionPay0019811
SyntenyPay0019811
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTGTGAAGCAAAATCCAAAGTGGTGATTCCCACAATAGATTTGTCAAAATTAGAGGTAGGCACAAAAGAATGGGGTTTGGCATGCAAAGAGATGAGATTTGGTTTAGAAGAATTTGGTTGTTTTTCAGTGGTTTATGACAAAGTTTCTTTGGAGCTTCATAACTCCATTGTTGAAGCAACAAAAGAGCTTTTTCATCTCCCAATTGAAACAAGAATGAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCATTTCTTCCTCTCTATGAATCCATGGCTATTGATAATCCAATTCAGCTAAAATCAACTCAGAGTTTTGCCAATCTCATGTGGCCTACTAGAGATAACAATCACTTCTGGTAATTTCATTATTAACACATACATACACACTATATATAGTACTTTTGTTGTCCATTGTATCTATTTAGTTTCACTTAAATCTTAACTCTGATTTTCACTTAAATCGCAACAAATCTTTTAGGGGAACAAAAATTGATTATGAACCAATTTTAATAATACCTCAAACTTTCAATTCTCGGTGTCTATGTTGATGACAGTGTAACTGTTGAACGTTTCTCAACATTGGTGGCGAAATTGGAGAAGATGGTAACAAAAATGGTGTTGGAAAGCTATGGAGTATCAAAGGTTGTGAATGAACCAATAATGGAGTCCACAAATTACCTTCTTCGGCTCTTCAAATACAGAGTAGCTGAGAAAGATGAAACCGATGTTGGTTTGCATTCCCATACAGATCTAACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGTCTTCAAATTCAATCACTTAATGATGATCAATGGCTCGATGTTCACCCCTCATCCCATTGCTCCTTCATTGTCATGGCTGGCGATGCACTCATGGTAAGTACTACACACCAATCCTTTCTTTTTTTCTTTTGTTTTTCGCAAAATTTAGTTCATGAGCATTAGTTATCGGAATAGAGATCTTTAAAGCATAAATTTTGTCTCGTGAAACCATATGATTAATTGGTGGGTGTATGTAGGCATGGAGCAACGAGAGAATAAGGTCTTGTAGACATAGAGTGATAATGAGAGGAGAGGAAACAAGATACTCAATTGGGATGTTTTCATTCAAGAATGGGACAATTGAAGTACCTCAAGGGTTTGTGGACGATGCCAACCCACTACGTTACAAGCCATTTAATCACTATGACTTCCTTACTTATGACAAAGCTAATGCTTCTCATAATACTATCTCTCGTATCAAAGATTATTGTGGCCTACTTTGA

mRNA sequence

ATGGTTTGTGAAGCAAAATCCAAAGTGGTGATTCCCACAATAGATTTGTCAAAATTAGAGGTAGGCACAAAAGAATGGGGTTTGGCATGCAAAGAGATGAGATTTGGTTTAGAAGAATTTGGTTGTTTTTCAGTGGTTTATGACAAAGTTTCTTTGGAGCTTCATAACTCCATTGTTGAAGCAACAAAAGAGCTTTTTCATCTCCCAATTGAAACAAGAATGAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCATTTCTTCCTCTCTATGAATCCATGGCTATTGATAATCCAATTCAGCTAAAATCAACTCAGAGTTTTGCCAATCTCATGTGGCCTACTAGAGATAACAATCACTTCTGTGTAACTGTTGAACGTTTCTCAACATTGGTGGCGAAATTGGAGAAGATGGTAACAAAAATGGTGTTGGAAAGCTATGGAGTATCAAAGGTTGTGAATGAACCAATAATGGAGTCCACAAATTACCTTCTTCGGCTCTTCAAATACAGAGTAGCTGAGAAAGATGAAACCGATGTTGGTTTGCATTCCCATACAGATCTAACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGTCTTCAAATTCAATCACTTAATGATGATCAATGGCTCGATGTTCACCCCTCATCCCATTGCTCCTTCATTGTCATGGCTGGCGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCTTGTAGACATAGAGTGATAATGAGAGGAGAGGAAACAAGATACTCAATTGGGATGTTTTCATTCAAGAATGGGACAATTGAAGTACCTCAAGGGTTTGTGGACGATGCCAACCCACTACGTTACAAGCCATTTAATCACTATGACTTCCTTACTTATGACAAAGCTAATGCTTCTCATAATACTATCTCTCGTATCAAAGATTATTGTGGCCTACTTTGA

Coding sequence (CDS)

ATGGTTTGTGAAGCAAAATCCAAAGTGGTGATTCCCACAATAGATTTGTCAAAATTAGAGGTAGGCACAAAAGAATGGGGTTTGGCATGCAAAGAGATGAGATTTGGTTTAGAAGAATTTGGTTGTTTTTCAGTGGTTTATGACAAAGTTTCTTTGGAGCTTCATAACTCCATTGTTGAAGCAACAAAAGAGCTTTTTCATCTCCCAATTGAAACAAGAATGAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCATTTCTTCCTCTCTATGAATCCATGGCTATTGATAATCCAATTCAGCTAAAATCAACTCAGAGTTTTGCCAATCTCATGTGGCCTACTAGAGATAACAATCACTTCTGTGTAACTGTTGAACGTTTCTCAACATTGGTGGCGAAATTGGAGAAGATGGTAACAAAAATGGTGTTGGAAAGCTATGGAGTATCAAAGGTTGTGAATGAACCAATAATGGAGTCCACAAATTACCTTCTTCGGCTCTTCAAATACAGAGTAGCTGAGAAAGATGAAACCGATGTTGGTTTGCATTCCCATACAGATCTAACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGTCTTCAAATTCAATCACTTAATGATGATCAATGGCTCGATGTTCACCCCTCATCCCATTGCTCCTTCATTGTCATGGCTGGCGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCTTGTAGACATAGAGTGATAATGAGAGGAGAGGAAACAAGATACTCAATTGGGATGTTTTCATTCAAGAATGGGACAATTGAAGTACCTCAAGGGTTTGTGGACGATGCCAACCCACTACGTTACAAGCCATTTAATCACTATGACTTCCTTACTTATGACAAAGCTAATGCTTCTCATAATACTATCTCTCGTATCAAAGATTATTGTGGCCTACTTTGA

Protein sequence

MVCEAKSKVVIPTIDLSKLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTISRIKDYCGLL
Homology
BLAST of Pay0019811 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-56
Identity = 120/301 (39.87%), Postives = 178/301 (59.14%), Query Frame = 0

Query: 11  IPTIDLS--KLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEATKELFHL 70
           +P ID S   L+ G+ +W     ++   LE++GCF   +DK+S+EL+ S+ EA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFEL 73

Query: 71  PIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVT 130
           PI T+ +N S KP+HGY        LYES+ ID+   L+    F   +WP   N     T
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGIDDANVLEKVNDFTQQLWPDHGNKSISET 133

Query: 131 VERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKD-----ETD 190
           +  FS  + +L+ MV +M++ES+G+   ++E  + ST YL RL KY     D     ET 
Sbjct: 134 IHLFSEQLVELDLMVRRMIMESFGIENYIDEH-LNSTYYLTRLMKYTSPPDDDDDDEETK 193

Query: 191 VGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERI 250
           +GL SHTD    +I+HQ+ + GL++++  DD+W+ V PS   S +VM GD+L A  N R+
Sbjct: 194 LGLRSHTDKNIITILHQYQVDGLEVKT-KDDKWIKVKPSQD-SVLVMVGDSLCALLNGRL 253

Query: 251 RSCRHRVIMRGEETRYSIGMFSF-KNGT-IEVPQGFVDDANPLRYKPFNHYDFLTYDKAN 303
            S  HRVIM G++TRYS G+FS  K G  I+ P+  VD  +P  +KPF + DFL + +  
Sbjct: 254 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 307

BLAST of Pay0019811 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 5.2e-56
Identity = 119/302 (39.40%), Postives = 178/302 (58.94%), Query Frame = 0

Query: 11  IPTIDLS--KLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEATKELFHL 70
           +P ID S   L+ G+ +W     ++   LE++GCF   +DK+S+EL+ S+ EA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 73

Query: 71  PIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVT 130
           PI T+ +N S KP+HGY        LYES+ I++   L+    F   +WP   N     T
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISET 133

Query: 131 VERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKD------ET 190
           +  FS  + +L+ MV +M++ES+G+   ++E  + ST YL RL KY     D      ET
Sbjct: 134 IHLFSEQLVELDLMVRRMIMESFGIENYIDEH-LNSTYYLTRLMKYTSPPDDDDDDDEET 193

Query: 191 DVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNER 250
            +GL SHTD    +I+HQ+ + GL++++  DD+W+ V PS   S +VM GD+L A  N R
Sbjct: 194 KLGLRSHTDKNIITILHQYQVDGLEVKT-KDDKWIKVKPSQD-SVLVMVGDSLCALLNGR 253

Query: 251 IRSCRHRVIMRGEETRYSIGMFSF-KNGT-IEVPQGFVDDANPLRYKPFNHYDFLTYDKA 303
           + S  HRVIM G++TRYS G+FS  K G  I+ P+  VD  +P  +KPF + DFL + + 
Sbjct: 254 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 308

BLAST of Pay0019811 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.2e-55
Identity = 119/300 (39.67%), Postives = 178/300 (59.33%), Query Frame = 0

Query: 11  IPTIDLS--KLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEATKELFHL 70
           +P ID S   L+ G+ +W     ++   LE++GCF   +DK+S+EL+ S+ EA ++LF L
Sbjct: 7   LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 66

Query: 71  PIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVT 130
           PI T+ +N S K +HGY        LYES+ I++   L+    F   +WP   N     T
Sbjct: 67  PIPTKQRNVSSKLFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISET 126

Query: 131 VERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKD----ETDV 190
           +  FS  + +L+ MV +M++ES+G+ K ++E  + ST YL RL KY     D    ET +
Sbjct: 127 IHLFSEQLVELDLMVRRMIMESFGIEKYIDEH-LNSTYYLTRLMKYTSPPDDDDDEETKL 186

Query: 191 GLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIR 250
           GL SHTD    +I+HQ+ + GL++++  DD+W+ V PS   S +VM GD+L A  N R+ 
Sbjct: 187 GLRSHTDKNIITILHQYQVDGLEVKT-KDDKWIKVKPSQD-SVLVMVGDSLCALLNGRLH 246

Query: 251 SCRHRVIMRGEETRYSIGMFSF-KNGT-IEVPQGFVDDANPLRYKPFNHYDFLTYDKANA 303
           S  HRVIM G++TRYS G+FS  K G  I+ P+  VD  +P  +KPF + DFL + +  A
Sbjct: 247 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 299

BLAST of Pay0019811 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 9.9e-47
Identity = 107/305 (35.08%), Postives = 157/305 (51.48%), Query Frame = 0

Query: 11  IPTIDLS--KLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEATKELFHL 70
           +P IDLS   L+ GT  W     ++R  LEE+GCF  V D VS EL  ++     EL +L
Sbjct: 9   LPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKGHELLNL 68

Query: 71  PIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVT 130
           P+ET+M N ++  Y G+    S   L E M +     L+  + F NLMWP   N  FC T
Sbjct: 69  PLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-EGNKDFCET 128

Query: 131 VERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHS 190
           V  +   +A+++ ++  MV ES+G+ K   +   ESTNYLL   +Y+   KD     L  
Sbjct: 129 VYSYGKRMAEVDHILKMMVFESFGMEKHF-DSFCESTNYLLHFMRYQQPGKDGRSPALSL 188

Query: 191 HTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRH 250
           H D +  +I++Q+ + GL+ ++  D +W+     +H   IV+ GD  MAWSN R+ S  H
Sbjct: 189 HKDKSILTIVNQNDVKGLEFET-KDGEWILPTADNH---IVLLGDCFMAWSNGRLHSPLH 248

Query: 251 RVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTIS 310
           RV +   + R S   FSF    IE P   VD+ +PL + PF   + L Y         + 
Sbjct: 249 RVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAKAVC 304

Query: 311 RIKDY 314
            +K Y
Sbjct: 309 DLKQY 304

BLAST of Pay0019811 vs. ExPASy Swiss-Prot
Match: Q9ZTA2 (Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.5e-39
Identity = 112/395 (28.35%), Postives = 184/395 (46.58%), Query Frame = 0

Query: 11  IPTIDLS--KLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEATKELFHL 70
           +P I+L+   LE G+ +W     ++R  LE+FGCF   YDKVSLEL  SI++  +ELF L
Sbjct: 8   LPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFAL 67

Query: 71  PIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRD-NNHFCV 130
           P+ET+ +N   KPY GY    +   L ES+ I N   L++   F   +WP  D N +   
Sbjct: 68  PVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENINEFTQQLWPHGDGNENISK 127

Query: 131 TVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKY------------- 190
           T++ F+  + +++ MV +MV+ES+G+ K +++  ++ST Y                    
Sbjct: 128 TIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDH-LKSTAYATDELNIVGVEPNVGVKVNA 187

Query: 191 --------------------------------------------------------RVA- 250
                                                                    VA 
Sbjct: 188 DISDDVNANASVNAGVGANVNADTGVNDNLNVDANVAVGGGVNANTDLGVGVNVNSNVAV 247

Query: 251 -------------EKDETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSH 310
                        + +E  +GL  HTD   F+++ QH I GL++++  D++W+ V PS +
Sbjct: 248 NAKTGGDDVEANDDNEEKKLGLPCHTDKNLFTVLFQHEIEGLEVKT-KDEKWIRVKPSPN 307

Query: 311 CSFIVMAGDALMAWSNERIRSCRHRV-IMRGEETRYSIGMFSF--KNGTIEVPQGFVDDA 317
            +FIV+AGD+L A  N RIR+  HRV +   + TRY+  +F+    +  IE P+  VD+ 
Sbjct: 308 -TFIVIAGDSLCALMNGRIRAPYHRVRVTEKKRTRYTAAIFTCPKPDYVIEAPKELVDEK 367

BLAST of Pay0019811 vs. ExPASy TrEMBL
Match: A0A1S3CDV2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 3.6e-185
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MVCEAKSKVVIPTIDLSKLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVE 60
           MVCEAKSKVVIPTIDLSKLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVE
Sbjct: 1   MVCEAKSKVVIPTIDLSKLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVE 60

Query: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTR 120
           ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTR
Sbjct: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTR 120

Query: 121 DNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKD 180
           DNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKD
Sbjct: 121 DNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKD 180

Query: 181 ETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSN 240
           ETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSN
Sbjct: 181 ETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSN 240

Query: 241 ERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKA 300
           ERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKA
Sbjct: 241 ERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKA 300

Query: 301 NASHNTISRIKDYCGLL 318
           NASHNTISRIKDYCGLL
Sbjct: 301 NASHNTISRIKDYCGLL 317

BLAST of Pay0019811 vs. ExPASy TrEMBL
Match: A0A5A7USF0 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002400 PE=3 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 9.4e-141
Identity = 243/244 (99.59%), Postives = 244/244 (100.00%), Query Frame = 0

Query: 74  MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVTVERFS 133
           MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVTVERFS
Sbjct: 1   MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVTVERFS 60

Query: 134 TLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHSHTDLT 193
           TLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHSHTDLT
Sbjct: 61  TLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHSHTDLT 120

Query: 194 FFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRHRVIMR 253
           FFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRH+VIMR
Sbjct: 121 FFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRHKVIMR 180

Query: 254 GEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTISRIKDY 313
           GEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTISRIKDY
Sbjct: 181 GEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTISRIKDY 240

Query: 314 CGLL 318
           CGLL
Sbjct: 241 CGLL 244

BLAST of Pay0019811 vs. ExPASy TrEMBL
Match: A0A6J1GPR2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111456352 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 4.4e-130
Identity = 229/315 (72.70%), Postives = 260/315 (82.54%), Query Frame = 0

Query: 5   AKSKVVIPTIDLS---KLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEA 64
           ++++  +P IDLS   KLE GT  WG  CK MR GLEEFGCF VV+++VSLELHNS   A
Sbjct: 3   SETQAGVPVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGA 62

Query: 65  TKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRD 124
             ELF LPIETR+KNTSEK YHGYFG YSFLPLYESMAIDNP QL + QSFANLMWP + 
Sbjct: 63  VDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPDQG 122

Query: 125 NNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDE 184
           NN F  T++RFSTLVAKL+K+VT+MVLESYGVSK + +  ++ST  LLR FKYRV E+ E
Sbjct: 123 NNRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPEQHE 182

Query: 185 TDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNE 244
           TDVGLH+HTDLTFFSIIHQHLI GLQIQSL DDQW+D+ PSSHCSFIVMAGDALMAWSN+
Sbjct: 183 TDVGLHAHTDLTFFSIIHQHLIGGLQIQSL-DDQWIDLEPSSHCSFIVMAGDALMAWSND 242

Query: 245 RIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKAN 304
           RIRSCRHRV M+ EETRYS GMFSFKNGTI VP+  VDDANPLRYKPFNHYDFLT+DKA 
Sbjct: 243 RIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDKAK 302

Query: 305 ASHNTISRIKDYCGL 317
            S   + RI+DYC +
Sbjct: 303 GSKKFLYRIRDYCSI 316

BLAST of Pay0019811 vs. ExPASy TrEMBL
Match: A0A6J1JXN6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111488804 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.3e-129
Identity = 224/315 (71.11%), Postives = 261/315 (82.86%), Query Frame = 0

Query: 5   AKSKVVIPTIDLSK---LEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEA 64
           ++++  +P IDLSK   LE GT+ WG  CK MR GLEEFGCF VV+++VSLELHNS   A
Sbjct: 3   SETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGA 62

Query: 65  TKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRD 124
             ELF LPIETR+KNTSEK YHGYFG YSFLPLYESMAI+NP QL + QSFANLMWP + 
Sbjct: 63  VDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPAQG 122

Query: 125 NNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDE 184
           NNHF  T++RFSTL+AKL+K++T+MVLESYGV+K + +  ++ST  LLR FKYRV E+ E
Sbjct: 123 NNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEEHE 182

Query: 185 TDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNE 244
           TDVGLH+HTDLTFFSIIHQHLI GLQIQSL DDQW+D+ PS HCSF+VMAGDALMAWSN+
Sbjct: 183 TDVGLHAHTDLTFFSIIHQHLIGGLQIQSL-DDQWIDLEPSGHCSFVVMAGDALMAWSND 242

Query: 245 RIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKAN 304
           RIRSCRHRV M+ EETRYS GMFSFKNGTI VP+  VDDANPLRYKPFNH+DFLTYDKA 
Sbjct: 243 RIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKAK 302

Query: 305 ASHNTISRIKDYCGL 317
            S   + RI+DYC +
Sbjct: 303 GSKKFLYRIRDYCSI 316

BLAST of Pay0019811 vs. ExPASy TrEMBL
Match: A0A6J1CXE5 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111015077 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 9.1e-128
Identity = 228/321 (71.03%), Postives = 268/321 (83.49%), Query Frame = 0

Query: 1   MVCEAKSKVVIPTIDLS--KLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSI 60
           M  +AKSK  IPTI+LS  KL+VG++EW L  KE+R  LEEFGCF VVY+KVSLELHNSI
Sbjct: 1   MGTQAKSK--IPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSI 60

Query: 61  VEATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWP 120
            EATKELFHLPIETR++NTSEKPYHGYFG YSFLPLYESMA+DNP  L++ Q+F NLMWP
Sbjct: 61  FEATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWP 120

Query: 121 TRDNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAE 180
              N  FC TV  FSTLVAKLE+ VT+MVLESYGVSK V E  +EST +LLR FKYRV +
Sbjct: 121 A-GNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPK 180

Query: 181 KDETDVGLHSHTDLTFFSIIHQHLISGLQIQSL---NDDQWLDVHPSSHCSFIVMAGDAL 240
           +DETD+GLHSHTDLTF SI+HQHLI GLQ++SL   ND +W+D+ P    SFIVMAGDAL
Sbjct: 181 QDETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNNDGEWIDIDPVGRSSFIVMAGDAL 240

Query: 241 MAWSNERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFL 300
           MAWSN+RIR CRHRV+MRG+ETRYS+G+FSFK+GTI VP+  V+D +PL+YKPF+H++FL
Sbjct: 241 MAWSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFL 300

Query: 301 TYDKANASHNTISRIKDYCGL 317
            YDKANASH T SRIKDYCG+
Sbjct: 301 RYDKANASHKTCSRIKDYCGI 318

BLAST of Pay0019811 vs. NCBI nr
Match: XP_008461057.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo])

HSP 1 Score: 657.1 bits (1694), Expect = 7.5e-185
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MVCEAKSKVVIPTIDLSKLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVE 60
           MVCEAKSKVVIPTIDLSKLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVE
Sbjct: 1   MVCEAKSKVVIPTIDLSKLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVE 60

Query: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTR 120
           ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTR
Sbjct: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTR 120

Query: 121 DNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKD 180
           DNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKD
Sbjct: 121 DNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKD 180

Query: 181 ETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSN 240
           ETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSN
Sbjct: 181 ETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSN 240

Query: 241 ERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKA 300
           ERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKA
Sbjct: 241 ERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKA 300

Query: 301 NASHNTISRIKDYCGLL 318
           NASHNTISRIKDYCGLL
Sbjct: 301 NASHNTISRIKDYCGLL 317

BLAST of Pay0019811 vs. NCBI nr
Match: XP_004136073.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis sativus])

HSP 1 Score: 602.1 bits (1551), Expect = 2.9e-168
Identity = 294/317 (92.74%), Postives = 301/317 (94.95%), Query Frame = 0

Query: 1   MVCEAKSKVVIPTIDLSKLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVE 60
           MVCEAKSKVVIP IDLSKLE GTKEWGLAC+EMRFGLE+FGCFSVVYDKVSLELHNSI E
Sbjct: 1   MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFE 60

Query: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTR 120
           ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQL+ TQSFANLMWPT 
Sbjct: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTT 120

Query: 121 DNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVS-KVVNEPIMESTNYLLRLFKYRVAEK 180
           +NNHFCV VERFSTLVAKLE MVTKMVLESYGVS KVV+EPIMESTNYLLRLFKYRVAEK
Sbjct: 121 ENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEK 180

Query: 181 DETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWS 240
           DE DVGLHSHTDLTF SIIHQHLISGLQIQ L DDQWL+VHPSSHCSF VMAGDALMAWS
Sbjct: 181 DENDVGLHSHTDLTFLSIIHQHLISGLQIQLL-DDQWLEVHPSSHCSFTVMAGDALMAWS 240

Query: 241 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDK 300
           NERIRSCRHRVIMRGEETRYSIGMFSFKNG IEVPQ FVDDANPLRYKPF+HYDFLTYDK
Sbjct: 241 NERIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDK 300

Query: 301 ANASHNTISRIKDYCGL 317
           ANASH TISRIKDYCGL
Sbjct: 301 ANASHKTISRIKDYCGL 316

BLAST of Pay0019811 vs. NCBI nr
Match: XP_038898684.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 566.2 bits (1458), Expect = 1.7e-157
Identity = 271/314 (86.31%), Postives = 291/314 (92.68%), Query Frame = 0

Query: 4   EAKSKVVIPTIDLSKLEV-GTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEAT 63
           EAK+K V+PTIDLSKLE  GT+EWGLACKEMR GLEEFGCFSVVYD+VSLELHNSI EAT
Sbjct: 5   EAKAK-VLPTIDLSKLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEAT 64

Query: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDN 123
           KELFHLP+ETR+KNTSEKPYHGYFGGYS LPLYESM IDNPI+L++TQSFANLMWPT++N
Sbjct: 65  KELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKEN 124

Query: 124 NHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDET 183
           NHFC TVERFSTLVA+LEKMVTKMVLESYG+SK+VNEP MES NYLLR FKYR  EKDET
Sbjct: 125 NHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDET 184

Query: 184 DVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNER 243
           DVGLHSHTDLTFFSIIHQHLISGLQ+QSL DDQW+D+ PS HC FIVMAGDALMAWSNER
Sbjct: 185 DVGLHSHTDLTFFSIIHQHLISGLQVQSL-DDQWIDIQPSGHCYFIVMAGDALMAWSNER 244

Query: 244 IRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANA 303
           IRSCRHRVIMRGEETRYSIGMFSFKNGTI VP+  VDDANPLRYKPFNHYDFLTYDKA A
Sbjct: 245 IRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKA 304

Query: 304 SHNTISRIKDYCGL 317
           SH  +SRIKDYCG+
Sbjct: 305 SHQNVSRIKDYCGI 316

BLAST of Pay0019811 vs. NCBI nr
Match: KAA0058792.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo var. makuwa] >TYK10586.1 putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 509.6 bits (1311), Expect = 1.9e-140
Identity = 243/244 (99.59%), Postives = 244/244 (100.00%), Query Frame = 0

Query: 74  MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVTVERFS 133
           MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVTVERFS
Sbjct: 1   MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVTVERFS 60

Query: 134 TLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHSHTDLT 193
           TLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHSHTDLT
Sbjct: 61  TLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHSHTDLT 120

Query: 194 FFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRHRVIMR 253
           FFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRH+VIMR
Sbjct: 121 FFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRHKVIMR 180

Query: 254 GEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTISRIKDY 313
           GEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTISRIKDY
Sbjct: 181 GEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTISRIKDY 240

Query: 314 CGLL 318
           CGLL
Sbjct: 241 CGLL 244

BLAST of Pay0019811 vs. NCBI nr
Match: XP_031744410.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 [Cucumis sativus] >KAE8646456.1 hypothetical protein Csa_016394 [Cucumis sativus])

HSP 1 Score: 500.0 bits (1286), Expect = 1.5e-137
Identity = 251/316 (79.43%), Postives = 259/316 (81.96%), Query Frame = 0

Query: 1   MVCEAKSKVVIPTIDLSKLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVE 60
           MVCEAKSKVVIP IDLSKLE GTKEWGLAC+EMRFGLE+FGCFSVVYDKVSLELHNSI E
Sbjct: 1   MVCEAKSKVVIPIIDLSKLEEGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFE 60

Query: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTR 120
           ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQL+ TQSFANLMWPT 
Sbjct: 61  ATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTT 120

Query: 121 DNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKD 180
           +NNHFCV VERFSTLVAKLE MVTKMVLESYGVS                          
Sbjct: 121 ENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVS-------------------------- 180

Query: 181 ETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSN 240
                             ++HLISGLQIQ L DDQWL+VHPSSHCSF VMAGDALMAWSN
Sbjct: 181 ------------------NKHLISGLQIQLL-DDQWLEVHPSSHCSFTVMAGDALMAWSN 240

Query: 241 ERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKA 300
           ERIRSCRHRVIMRGEETRYSIGMFSFKNG IEVPQ FVDDANPLRYKPF+HYDFLTYDKA
Sbjct: 241 ERIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKA 271

Query: 301 NASHNTISRIKDYCGL 317
           NASH TISRIKDYCGL
Sbjct: 301 NASHKTISRIKDYCGL 271

BLAST of Pay0019811 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.0e-71
Identity = 131/308 (42.53%), Postives = 204/308 (66.23%), Query Frame = 0

Query: 11  IPTIDLSK--LEVGTKEWGLACKEMRFGLEEFGCFSV-VYDKVSLELHNSIVEATKELFH 70
           IPT+D S+  L+ GTK W    + +R  LEE+GCF + + DK  L+L + +  +  +LF 
Sbjct: 5   IPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLFD 64

Query: 71  LPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCV 130
           LP +T+MKN  +KP +GY G    LPL+ES+ IDN   L++T+SF  LMWP + N HF  
Sbjct: 65  LPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGNEHFSE 124

Query: 131 TVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLH 190
            + +++   A+L++MVT+MV +SY V K   +P +EST YLLR+ K R    +   +G  
Sbjct: 125 CLYKYAEFAAELDQMVTRMVFQSYNVEKYY-DPYIESTTYLLRVLKNRAPNNENPTLGFV 184

Query: 191 SHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCR 250
           +HTD +F +I+HQ  ++GL++++  + + ++++ SS   F+V+AGDALMAWSN+R+ S R
Sbjct: 185 THTDKSFTTILHQDQVNGLEMET-REGERININLSSPSLFMVVAGDALMAWSNDRVWSPR 244

Query: 251 HRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTI 310
           H+V++ GE  RYS+GMF+F NGT++VP+  +D  +PL YKPF+H   L + + +  + + 
Sbjct: 245 HQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKSE 304

Query: 311 SRIKDYCG 316
             IK YCG
Sbjct: 305 CPIKAYCG 309

BLAST of Pay0019811 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 1.9e-69
Identity = 128/309 (41.42%), Postives = 188/309 (60.84%), Query Frame = 0

Query: 11  IPTIDLS---KLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEATKELFH 70
           +P +DL+   +L+  T  W    +E    LEE+GCF  VYD V+ +L +SI  A +ELF 
Sbjct: 10  VPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELFD 69

Query: 71  LPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCV 130
           LP ET+ KN +EKPYHGY G    +PL+E + +D     +  Q F +LMWP + N+ FC 
Sbjct: 70  LPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRFCN 129

Query: 131 TVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLH 190
           TV  FS  VA+L+++V +M+ E+YGV K   E  + S  YLL+  KY    +  +     
Sbjct: 130 TVHTFSNAVAELDRLVVRMIFENYGVEKHY-ESHVGSKTYLLKFLKYLAPPESISMPAFP 189

Query: 191 SHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCR 250
            HTD TF SI+HQ+ ++GL+++S  D +W+ +      S++VMAGD  M WSN+RIRSC 
Sbjct: 190 QHTDKTFLSILHQNDVNGLEVKS-KDGEWISLQLPPK-SYVVMAGDISMGWSNDRIRSCE 249

Query: 251 HRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTI 310
           HRV M G++TRY++G+FSF    + +P+  VDD +PL YKPF++   + +          
Sbjct: 250 HRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREAN 309

Query: 311 SRIKDYCGL 317
           S +K YCG+
Sbjct: 310 STLKAYCGI 314

BLAST of Pay0019811 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.3e-65
Identity = 121/310 (39.03%), Postives = 193/310 (62.26%), Query Frame = 0

Query: 11  IPTIDLS--KLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEATKELFHL 70
           +P ID S   L+ G  EW L   +++  L+++G F   +D++  EL  S+  A +ELF L
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFDL 71

Query: 71  PIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVT 130
           P++T+++N S+KP+HGY G Y  +PLYESM ID+    +   +F   +WP + N  F  T
Sbjct: 72  PLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFSTT 131

Query: 131 VERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHS 190
           ++ FS  +++L+  + +M++ES+G+ K ++E  + STNYLLR+ KY+  + +ET VGL++
Sbjct: 132 IQSFSKKLSELDITIRRMIMESFGLDKYIDEH-LHSTNYLLRVMKYKGPDTEETKVGLNA 191

Query: 191 HTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRH 250
           HTD    +I++Q+ + GL++Q+  D  W+ V P+   SF VM GD+L A  N R+ S  H
Sbjct: 192 HTDKNIVTILYQNHVEGLEVQT-KDKNWIKVKPTQD-SFTVMIGDSLYALLNGRLHSPYH 251

Query: 251 RVIMRGEETRYSIGMFSFKNG--TIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNT 310
           RV+M G ETRYS+G+FS       +  P   VD+ +P  +KPF+H +FL +    A   +
Sbjct: 252 RVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQRS 311

Query: 311 ISRIKDYCGL 317
            S +K YCG+
Sbjct: 312 QSALKTYCGI 317

BLAST of Pay0019811 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.5e-61
Identity = 128/313 (40.89%), Postives = 179/313 (57.19%), Query Frame = 0

Query: 11  IPTIDLS--KLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEATKELFHL 70
           +P I+LS   L+  T+ W      +R  +E  G F   Y+     LH SI+EA KEL  L
Sbjct: 9   LPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILEAAKELLDL 68

Query: 71  PIETRMKNTSEKPYHGYFGGYS-FLPLYESMAIDNPIQLKSTQSFANLMWPT--RDNNHF 130
           P+E ++KN + K  HGY    S   P++E + ID    ++  + F+ LMWP    DN+ F
Sbjct: 69  PVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHDDNDRF 128

Query: 131 CVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVG 190
           C TV  ++ + A+LE++V +M+ ESY V K   E  +  T YLLRL KYR     E +  
Sbjct: 129 CETVHAYAKMQAELEQLVIRMLFESYNVEK-YTEKYIGGTRYLLRLLKYRRLPNGEPNRK 188

Query: 191 LHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRS 250
             SHTD +F SI+HQ+ I+GL ++S  +D W    PS    F+V+AGDA+MAWSN+RI++
Sbjct: 189 FISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPSP-TRFVVIAGDAIMAWSNDRIKA 248

Query: 251 CRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHN 310
           C H+V M   E RYS+G FSF+ G I  P+  VD  +PL Y PF+H   L Y +    H 
Sbjct: 249 CYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETLEVHL 308

Query: 311 TISR--IKDYCGL 317
              R   K YCG+
Sbjct: 309 KAHRTMTKAYCGI 319

BLAST of Pay0019811 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 219.5 bits (558), Expect = 3.7e-57
Identity = 114/313 (36.42%), Postives = 187/313 (59.74%), Query Frame = 0

Query: 11  IPTIDLS--KLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEATKELFHL 70
           +P +D S  KL  GT  W     ++R  +E  G F   +  VS +L ++++   KE+++L
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMKEMYYL 68

Query: 71  PIETRMKNTSEKPYHGYFG-GYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCV 130
           P + ++KN + K  HGY         ++ES+ ID   +L++ + F+ L+WP + N+ FC 
Sbjct: 69  PDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP-QGNDPFCQ 128

Query: 131 TVERFSTLVAKLEKMVTKMVLESYGV-SKVVNEPIMESTNYLLRLFKYRVAEKDETDVGL 190
           T   ++  +A+L++ V +M+ ESYG+  K  +    EST YLLR+  YR  +  E + G 
Sbjct: 129 TTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTGF 188

Query: 191 HSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSC 250
            SHTD +F SI+HQ+ + GLQ++++   QW+  +PS    F+V++G  L AWSN+RI++C
Sbjct: 189 VSHTDKSFMSILHQNHVGGLQLKTMT-GQWVGFNPSP-TRFVVLSGMGLTAWSNDRIKAC 248

Query: 251 RHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKA---NAS 310
            H+V+M  +E RYS+G FSF  GTI  P+  VDD +PLRY PF H   L + ++   +  
Sbjct: 249 YHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYINSLK 308

Query: 311 HNTISRIKDYCGL 317
            ++   ++ YCG+
Sbjct: 309 KSSEDLLQIYCGV 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q945B61.4e-5639.87Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
Q9ZTA35.2e-5639.40Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
Q944Z51.2e-5539.67Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
A0A0N9HQ369.9e-4735.08Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=... [more]
Q9ZTA21.5e-3928.35Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thali... [more]
Match NameE-valueIdentityDescription
A0A1S3CDV23.6e-185100.00probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX... [more]
A0A5A7USF09.4e-14199.59Putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo va... [more]
A0A6J1GPR24.4e-13072.70probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1JXN61.3e-12971.11probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1CXE59.1e-12871.03probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=367... [more]
Match NameE-valueIdentityDescription
XP_008461057.17.5e-185100.00PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumi... [more]
XP_004136073.12.9e-16892.74probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis sativus][more]
XP_038898684.11.7e-15786.31probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida][more]
KAA0058792.11.9e-14099.59putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo var.... [more]
XP_031744410.11.5e-13779.43probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 [Cucumis ... [more]
Match NameE-valueIdentityDescription
AT1G52790.12.0e-7142.532-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52800.11.9e-6941.422-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52820.11.3e-6539.032-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G15540.11.5e-6140.892-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G80320.13.7e-5736.422-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 11..87
e-value: 4.0E-11
score: 43.7
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 168..266
e-value: 1.6E-16
score: 60.6
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..304
e-value: 1.7E-57
score: 197.2
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 4..315
NoneNo IPR availablePANTHERPTHR47990:SF1692-OXOGLUTARATE-DEPENDENT DIOXYGENASEcoord: 4..315
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 10..297
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 164..269
score: 10.510512

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0019811.1Pay0019811.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding