Pay0019523 (gene) Melon (Payzawat) v1

Overview
NamePay0019523
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionRNA pseudouridine synthase 1
Locationchr05: 28933030 .. 28935690 (-)
RNA-Seq ExpressionPay0019523
SyntenyPay0019523
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATTTCTCAATTTTCCCCAAAACCTTCGCCAAATTCCCATTCACAAAACCCTTCTGTTTCCACAGATTCAAATCCGTCAGCAATAGCATGACGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCGGAGAGAATTACCCGGTCCCCTTATCGCCTCCACTGCCGGCCATATCCAAGAACTTAGAGCTCGCCAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAAGACGAGTGGCTCATTGCCGTCAATAAACCTCAAGGAATCTACTGTGAAAATGTTTTGGCTGCTGTTCCTCGTCTTCTTTGTGATTCGGCTAACGCTGGTTTGTGAATCTTACTGTGAAAGCGTTTTTTTTTTCAGACGAATTCAACTTTTTACTACATTTTGGATTTTCTAATCGAATGGTAATGGTGGGTTTGCAACTTTTAACTTGCTTTCGTTTGACGATTTGTGGAAAGGGTATGTATAATCATGGACTTCCCGTGGATAACGAAAGATGTTAGAACTGAGTTTTCGTTAAAATTGACGGGTTTTGAAAGATTTTGTTCCATATAGGTCATTTGCATTGATTTTCTAGATCAAACTTTTGTATATTGATAATGTAACTTGGTTTTTGTTGAAAGTTAGCTGAAAGTAATGGGCGAATGCGATGAAAGTTTAACAGAAGTTTAAAATTGTAATGCTTATCTATGTGTACGGTCCAATTTGTTGAAAATTTGAAGTTTGGAGTAATAATTGATTCGTATTGCCAAGCTTTTGGGTAGGATTTGATCTTTCATCAAATTTGTTTAGAACGAGTGATTTTCTATTGATTTAAGCTGATTAAAAATGCAATTCTCAAGTTCTATTTCCTCATCTGTAACTATCTTCTTGTAATTATTATGTAGGTGTTATTTTGCATAGTTGGAGTCTCTTCATATAGGGAGAGGCGCCTTTTTTCTGGGCTGTGCTTTTATTTTTATGCCGGTGTATTTTTTCTTTTTTTTTCCAATAAAGGCCGGTGTTTCTAAAAGAAAATAATACGTTTACAGAAGAAGCAAGGGCGAGTAACTGATTTCTGATTCTTTTCCTACAATCTACATAGCCTAGCAATGTTGCTGATTGTTAGATAGTTGATATAAATGTTTTAGATGTATCCTGGTTGTTGAAGAGAGAAGGGTGCTTGAATGTTTCTGAAGTGTTTTAACGATTGTCCAAAACAATGCCATGTAATTACATCTTTTGCTAATTCAATCATTATCAATTTGTTGGGAGTGTTCTTAGCTCGAATTCTTCATATGCAGGGATCAAAACCAATTTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACGAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACCGACCACACGGTTTCAAAATCATATATCGCCTTCTGTGTTGGCACATCTCCAAAATGGAAAAAAATCCACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCATAGTCAGAGATATGGAAACATATTTTGAAGTACTGTCTGTAAATGGAAAAAACACCATGGAGGAATTACAAAAATTCAGGAAAGAGGAAGAAGAAACTATTGTAGTCCATACAAAATCTTTGGTGGACATTGATAGTCAGAAGGACGAGGTCTTGATAAGAGCAAGACCTCGCAGTGGAAGAACTCATCAAATCCGACTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGGAAAACTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTACTTTGTGCATCCTATTACAGGTATTCCTCTCAAACTACAAGCTCCTTTACCATCATGGGCCAGCCAAGCGTTGCAGCCCCAACAACAAGAAGTGAATTCTCCACAGTCCTTCAAAACTATGCCTTAAGAAAATCATTGGCGCAATACAGTTAAGTGAGAACCAAATATATCCCTTATTATACAAGGAGATTCGGTGTTGAAGAATGTCGCTGAAGCAAAATGGAGCGATCTTCAATCAAATTCATAAAGTGCGACTGCAGACAGTTTTCGTACTGTCAAGTTGATGTCTGCAATTTTTCATCTATTGAATGAGCTCTTGTTTGCACGACAGTGTCTACAAAAATATCAGCAGTCGCAAACCAAGAAACCATCCGAGTTCTTGAAAATTTGAGCTCGAAATGGATTCTGGAAATGACCGAAGAATCATGTGTGTCAAAGGCTGATAAATTTGTGCAAAGCCCTTTGGAAATGGTTCTTCTGCAGTGGTTTTATCGATAAAATTGATGTACGGTGCTAAAACAGCCAAATTCTGTGGTTGGCATGTTGATTTATATTGAGGCTCAATTGCTATCCGACTCCCCTTCCATCCATTTGTGCAACTTTGTCATCAAAATATGAACAATTTGTTATGGTGTTTATGCGGCTATGAATTATAAATAGAAGAATGAGAAACATGTTGTGGTTTATGTACATTTTTATTGATAGGTGTTTCAAATAGTTAAAGAATGAAACTTGACTGCTGGTTCTTTATC

mRNA sequence

CAATTTCTCAATTTTCCCCAAAACCTTCGCCAAATTCCCATTCACAAAACCCTTCTGTTTCCACAGATTCAAATCCGTCAGCAATAGCATGACGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCGGAGAGAATTACCCGGTCCCCTTATCGCCTCCACTGCCGGCCATATCCAAGAACTTAGAGCTCGCCAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAAGACGAGTGGCTCATTGCCGTCAATAAACCTCAAGGAATCTACTGTGAAAATGTTTTGGCTGCTGTTCCTCGTCTTCTTTGTGATTCGGCTAACGCTGGGATCAAAACCAATTTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACGAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACCGACCACACGGTTTCAAAATCATATATCGCCTTCTGTGTTGGCACATCTCCAAAATGGAAAAAAATCCACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCATAGTCAGAGATATGGAAACATATTTTGAAGTACTGTCTGTAAATGGAAAAAACACCATGGAGGAATTACAAAAATTCAGGAAAGAGGAAGAAGAAACTATTGTAGTCCATACAAAATCTTTGGTGGACATTGATAGTCAGAAGGACGAGGTCTTGATAAGAGCAAGACCTCGCAGTGGAAGAACTCATCAAATCCGACTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGGAAAACTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTACTTTGTGCATCCTATTACAGGTATTCCTCTCAAACTACAAGCTCCTTTACCATCATGGGCCAGCCAAGCGTTGCAGCCCCAACAACAAGAAGTGAATTCTCCACAGTCCTTCAAAACTATGCCTTAAGAAAATCATTGGCGCAATACAGTTAAGTGAGAACCAAATATATCCCTTATTATACAAGGAGATTCGGTGTTGAAGAATGTCGCTGAAGCAAAATGGAGCGATCTTCAATCAAATTCATAAAGTGCGACTGCAGACAGTTTTCGTACTGTCAAGTTGATGTCTGCAATTTTTCATCTATTGAATGAGCTCTTGTTTGCACGACAGTGTCTACAAAAATATCAGCAGTCGCAAACCAAGAAACCATCCGAGTTCTTGAAAATTTGAGCTCGAAATGGATTCTGGAAATGACCGAAGAATCATGTGTGTCAAAGGCTGATAAATTTGTGCAAAGCCCTTTGGAAATGGTTCTTCTGCAGTGGTTTTATCGATAAAATTGATGTACGGTGCTAAAACAGCCAAATTCTGTGGTTGGCATGTTGATTTATATTGAGGCTCAATTGCTATCCGACTCCCCTTCCATCCATTTGTGCAACTTTGTCATCAAAATATGAACAATTTGTTATGGTGTTTATGCGGCTATGAATTATAAATAGAAGAATGAGAAACATGTTGTGGTTTATGTACATTTTTATTGATAGGTGTTTCAAATAGTTAAAGAATGAAACTTGACTGCTGGTTCTTTATC

Coding sequence (CDS)

ATGACGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCGGAGAGAATTACCCGGTCCCCTTATCGCCTCCACTGCCGGCCATATCCAAGAACTTAGAGCTCGCCAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAAGACGAGTGGCTCATTGCCGTCAATAAACCTCAAGGAATCTACTGTGAAAATGTTTTGGCTGCTGTTCCTCGTCTTCTTTGTGATTCGGCTAACGCTGGGATCAAAACCAATTTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACGAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACCGACCACACGGTTTCAAAATCATATATCGCCTTCTGTGTTGGCACATCTCCAAAATGGAAAAAAATCCACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCATAGTCAGAGATATGGAAACATATTTTGAAGTACTGTCTGTAAATGGAAAAAACACCATGGAGGAATTACAAAAATTCAGGAAAGAGGAAGAAGAAACTATTGTAGTCCATACAAAATCTTTGGTGGACATTGATAGTCAGAAGGACGAGGTCTTGATAAGAGCAAGACCTCGCAGTGGAAGAACTCATCAAATCCGACTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGGAAAACTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTACTTTGTGCATCCTATTACAGGTATTCCTCTCAAACTACAAGCTCCTTTACCATCATGGGCCAGCCAAGCGTTGCAGCCCCAACAACAAGAAGTGAATTCTCCACAGTCCTTCAAAACTATGCCTTAA

Protein sequence

MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSPQSFKTMP
Homology
BLAST of Pay0019523 vs. ExPASy Swiss-Prot
Match: Q2QNM3 (RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0560500 PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 8.5e-102
Identity = 183/300 (61.00%), Postives = 226/300 (75.33%), Query Frame = 0

Query: 14  STGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIY 73
           +TGE YPVP+SPP PA SK++EL RAM AS++S+  A S+  V++EDEWL  V+KP G+Y
Sbjct: 47  ATGE-YPVPVSPPYPAASKDVELRRAMTASARSA--AYSSAPVVFEDEWLAVVDKPAGVY 106

Query: 74  CENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH 133
           C+ +L+A+P   C +A  G +   P LHLANRLDRDTSG+MVITK +KVA KLVKAFT+H
Sbjct: 107 CDALLSALP---CSAATLGDEATKPNLHLANRLDRDTSGLMVITKCNKVAGKLVKAFTEH 166

Query: 134 TVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFE 193
            V K+Y+A C+G  P W+KI + SGHGRSK G WRVYA +DVGRSLPGGS+VRDM T FE
Sbjct: 167 KVKKTYLALCIGYPPAWEKIKICSGHGRSKHGAWRVYAMSDVGRSLPGGSVVRDMSTRFE 226

Query: 194 VLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDE----VLIRARPRSGRTHQIR 253
           VL +NGK    E   F  +E E+I V  K+  D+ S  DE    +L+RA P+SGRTHQIR
Sbjct: 227 VLGINGKGQFREPSNFEVDETESITVQEKA-ADLTSDGDEKNSIILVRAYPQSGRTHQIR 286

Query: 254 LHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ 310
           LHCQYLG PIRGDVKY GV EWNG  YD H LHAESL FVHP+TG+P+  ++PLPSWA++
Sbjct: 287 LHCQYLGFPIRGDVKYSGVIEWNGVDYDGHALHAESLSFVHPVTGLPVTFRSPLPSWANE 339

BLAST of Pay0019523 vs. ExPASy Swiss-Prot
Match: Q7XA65 (RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 8.8e-99
Identity = 181/294 (61.56%), Postives = 223/294 (75.85%), Query Frame = 0

Query: 18  NYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENV 77
           NYP P+S P P ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 78  LAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 137
           L + P+++ DS++        E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + K
Sbjct: 99  LRSAPQIVLDSSS--------EYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRK 158

Query: 138 SYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSV 197
           SYIA C+G+SP W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+SV
Sbjct: 159 SYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSV 218

Query: 198 NG-KNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDE--VLIRARPRSGRTHQIRLHCQY 257
           N  KN   EL+    +    IV   +  +      D+  V++RA PRSGRTHQIRLHCQY
Sbjct: 219 NSVKNESCELE----DVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQY 278

Query: 258 LGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWAS 309
           LGIPIRGDVKY GV EWNG+T++ HELHAE L   HP+TG  + ++APLP WA+
Sbjct: 279 LGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

BLAST of Pay0019523 vs. ExPASy Swiss-Prot
Match: Q68XB2 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=rluC PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.6e-18
Identity = 80/256 (31.25%), Postives = 117/256 (45.70%), Query Frame = 0

Query: 56  VIYEDEWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMV 115
           +IYED+ LIA+NKP  +  +    +   L  DSA   +     +  L +RLD++TSG+++
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSALKYLNYQGADFKLVHRLDKETSGLLL 150

Query: 116 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 175
           I K++  + KL  AF +  V K Y A   G   K   I VKS  G+SK    R++   D+
Sbjct: 151 IAKNYLSSVKLHDAFKEKLVIKKYFAITYGRPVKNVGI-VKSNIGKSK---GRMFKITDI 210

Query: 176 GRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVL 235
                               S NGK                 + + K L  +++  +  L
Sbjct: 211 D-------------------SDNGK---------------LAITYYKLLKSLNN--NLFL 270

Query: 236 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGI 295
           I   P +GR HQ+RLH Q LG PI GD KY          Y    LHA ++Y    I G 
Sbjct: 271 IEFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEVMPYSKY--MFLHANNIYLSESIVGK 302

Query: 296 PLKLQAPLPSWASQAL 312
            +KL+A LP + ++ L
Sbjct: 331 EIKLEAKLPFYFTRRL 302

BLAST of Pay0019523 vs. ExPASy Swiss-Prot
Match: Q87S65 (Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) OX=223926 GN=rluD PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 3.8e-17
Identity = 81/292 (27.74%), Postives = 126/292 (43.15%), Query Frame = 0

Query: 55  DVIYEDEWLIAVNKPQ--------GIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRL 114
           D++YED+ +I +NKP+        G     VL A+     D A       +P   + +RL
Sbjct: 85  DIVYEDDDIIVINKPRDFVVHPGAGTPDGTVLNALLHHYPDIA------EVPRAGIVHRL 144

Query: 115 DRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGV 174
           D+DT+G+MV+ K+    ++LV+A     +++ Y A  +G      K+    G   +K   
Sbjct: 145 DKDTTGLMVVAKTVPAQTRLVRALQKRNITREYEAIAIGRMTAGGKVDQPIGRHSTK--- 204

Query: 175 WRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVD 234
             + A A +G         +   T++ V            + FR+        HT+    
Sbjct: 205 RTLMAVAPLG---------KPAVTHYRV-----------AEHFRE--------HTR---- 264

Query: 235 IDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEG-----------VTEWNGKT 294
                    IR R  +GRTHQIR+H  YL  P+ GD  Y G           +T+   + 
Sbjct: 265 ---------IRLRLETGRTHQIRVHMSYLQHPLLGDTAYGGRARIPTGASQELTDMI-RG 324

Query: 295 YDSHELHAESLYFVHPITGIPLKLQAPLPS---WASQALQPQQQEVNSPQSF 325
           +D   LHA  L F HPITG  L+  AP+P      ++AL+   +E   P  F
Sbjct: 325 FDRQALHAVMLRFEHPITGEELEFHAPVPDDMVAMTEALRKDTEEYGLPDEF 325

BLAST of Pay0019523 vs. ExPASy Swiss-Prot
Match: Q9ZDR7 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=rluC PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 3.2e-16
Identity = 72/256 (28.12%), Postives = 113/256 (44.14%), Query Frame = 0

Query: 56  VIYEDEWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMV 115
           +IYED+ LIA+NKP  +  +    +   L  DSA   +     +  L +RLD++TSG+++
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSALKYLNYQGADFKLVHRLDKETSGLLL 150

Query: 116 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 175
           I K++  + KL  AF +  V K Y A   G  P      VKS  G+S             
Sbjct: 151 IAKNYLSSVKLHNAFKEKLVIKKYFAITYG-RPVKNVGTVKSNIGKS------------- 210

Query: 176 GRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVL 235
                                   K  + ++     ++ +  + + K L  +++  +  L
Sbjct: 211 ------------------------KRRLSKIANIDSDDGKLAITYYKLLKSLNN--NLFL 270

Query: 236 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGI 295
           I   P +GR HQ+RLH Q LG PI GD KY          Y    LHA ++Y    I G 
Sbjct: 271 IEFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEIMPYSKY--MFLHANNIYLSETIVGK 302

Query: 296 PLKLQAPLPSWASQAL 312
            +KL+A LP + ++ +
Sbjct: 331 EIKLEAKLPFYFTRRI 302

BLAST of Pay0019523 vs. ExPASy TrEMBL
Match: A0A5A7TX25 (RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001190 PE=4 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 5.8e-186
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60
           MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120
           EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180
           KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240
           GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 329
           APLPSWASQALQPQQQEVNSPQSFKTMP
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 328

BLAST of Pay0019523 vs. ExPASy TrEMBL
Match: A0A1S3BW87 (RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 5.8e-186
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60
           MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120
           EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180
           KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240
           GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 329
           APLPSWASQALQPQQQEVNSPQSFKTMP
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 328

BLAST of Pay0019523 vs. ExPASy TrEMBL
Match: A0A0A0LJY6 (PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G033960 PE=4 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.2e-177
Identity = 313/328 (95.43%), Postives = 320/328 (97.56%), Query Frame = 0

Query: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60
           MTDSSEPVTGNCPST ENYPVPLSPPLPAISKNLELARAM ASSKSSL+ALSANDVIYED
Sbjct: 39  MTDSSEPVTGNCPSTAENYPVPLSPPLPAISKNLELARAMVASSKSSLYALSANDVIYED 98

Query: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120
           EWLIAVNKPQGIYCENVLAAVPRLL DSANAGIKT+LPELHLANRLDRDTSGVMVITKSH
Sbjct: 99  EWLIAVNKPQGIYCENVLAAVPRLLGDSANAGIKTSLPELHLANRLDRDTSGVMVITKSH 158

Query: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180
           KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 159 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 218

Query: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240
           GGS+VRDMETYFEVLSVNGKNTMEELQKFR+ EEETIVVHTKSLVDIDS KDE+LIRARP
Sbjct: 219 GGSVVRDMETYFEVLSVNGKNTMEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARP 278

Query: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWN K YDSHELHAESLYFVHP+TGIPLKLQ
Sbjct: 279 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQ 338

Query: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 329
           APLPSWASQALQPQQ EVNSPQSFKTMP
Sbjct: 339 APLPSWASQALQPQQHEVNSPQSFKTMP 366

BLAST of Pay0019523 vs. ExPASy TrEMBL
Match: A0A6J1DQ12 (RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 7.4e-157
Identity = 279/330 (84.55%), Postives = 305/330 (92.42%), Query Frame = 0

Query: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60
           M D S PVTGN P+TG+NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANA-----GIKTNLPELHLANRLDRDTSGVMV 120
           EWLIAVNKPQG+YCE+VLA+VPRLL DSA A     GI+ +LPELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGIQISLPELHLANRLDRDTSGVMV 120

Query: 121 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 180
           ITKSHKVASKLVKAFTDH VSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 180

Query: 181 GRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVL 240
           GRSLPGGS+VRDMETYFEVLSVNG++TM+EL + RK+EEETIVV +KSL+DID+ KDE+L
Sbjct: 181 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 240

Query: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGI 300
           IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG  YDSHELHAESL+FVHP+TGI
Sbjct: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 300

Query: 301 PLKLQAPLPSWASQALQPQQQEVNSPQSFK 326
           PLKLQAPLPSWASQAL  QQQEV+SP + K
Sbjct: 301 PLKLQAPLPSWASQALLSQQQEVSSPSTLK 330

BLAST of Pay0019523 vs. ExPASy TrEMBL
Match: A0A6J1DT56 (RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.1e-155
Identity = 279/340 (82.06%), Postives = 305/340 (89.71%), Query Frame = 0

Query: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60
           M D S PVTGN P+TG+NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAG---------------IKTNLPELHLANR 120
           EWLIAVNKPQG+YCE+VLA+VPRLL DSA AG               I+ +LPELHLANR
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGTTTLCLCLARIQISLPELHLANR 120

Query: 121 LDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFG 180
           LDRDTSGVMVITKSHKVASKLVKAFTDH VSKSYIAFCVG++PKWKKI+VKSGHGRSKFG
Sbjct: 121 LDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 180

Query: 181 VWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLV 240
           VWRVYAAADVGRSLPGGS+VRDMETYFEVLSVNG++TM+EL + RK+EEETIVV +KSL+
Sbjct: 181 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLI 240

Query: 241 DIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAES 300
           DID+ KDE+LIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG  YDSHELHAES
Sbjct: 241 DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES 300

Query: 301 LYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSPQSFK 326
           L+FVHP+TGIPLKLQAPLPSWASQAL  QQQEV+SP + K
Sbjct: 301 LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSPSTLK 340

BLAST of Pay0019523 vs. NCBI nr
Match: XP_008453651.1 (PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa] >TYJ96474.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 659.8 bits (1701), Expect = 1.2e-185
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60
           MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120
           EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180
           KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240
           GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 329
           APLPSWASQALQPQQQEVNSPQSFKTMP
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 328

BLAST of Pay0019523 vs. NCBI nr
Match: XP_004144814.3 (RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein Csa_021313 [Cucumis sativus])

HSP 1 Score: 631.3 bits (1627), Expect = 4.6e-177
Identity = 313/328 (95.43%), Postives = 320/328 (97.56%), Query Frame = 0

Query: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60
           MTDSSEPVTGNCPST ENYPVPLSPPLPAISKNLELARAM ASSKSSL+ALSANDVIYED
Sbjct: 39  MTDSSEPVTGNCPSTAENYPVPLSPPLPAISKNLELARAMVASSKSSLYALSANDVIYED 98

Query: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120
           EWLIAVNKPQGIYCENVLAAVPRLL DSANAGIKT+LPELHLANRLDRDTSGVMVITKSH
Sbjct: 99  EWLIAVNKPQGIYCENVLAAVPRLLGDSANAGIKTSLPELHLANRLDRDTSGVMVITKSH 158

Query: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180
           KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 159 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 218

Query: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240
           GGS+VRDMETYFEVLSVNGKNTMEELQKFR+ EEETIVVHTKSLVDIDS KDE+LIRARP
Sbjct: 219 GGSVVRDMETYFEVLSVNGKNTMEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARP 278

Query: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWN K YDSHELHAESLYFVHP+TGIPLKLQ
Sbjct: 279 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQ 338

Query: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 329
           APLPSWASQALQPQQ EVNSPQSFKTMP
Sbjct: 339 APLPSWASQALQPQQHEVNSPQSFKTMP 366

BLAST of Pay0019523 vs. NCBI nr
Match: XP_038889468.1 (RNA pseudouridine synthase 1 [Benincasa hispida])

HSP 1 Score: 597.0 bits (1538), Expect = 9.5e-167
Identity = 296/321 (92.21%), Postives = 308/321 (95.95%), Query Frame = 0

Query: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60
           M DSSEPVTGNCPST ENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 37  MADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 96

Query: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120
           EWLIAVNKPQGIYCE+VLAAVPRLL DSA AGIKT+LPELHLANRLDRDTSGVMVITKSH
Sbjct: 97  EWLIAVNKPQGIYCESVLAAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSH 156

Query: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180
           KVASKLVKAFTDHTVSKSYIAFCVG++PKWKKI++KSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 157 KVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLP 216

Query: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240
           GGS+VRDMETYFEVLS+NG+N MEE QKF K +EET+VV TKSLVDID QKDE+LIRARP
Sbjct: 217 GGSVVRDMETYFEVLSINGQNAMEEFQKFGK-DEETVVVQTKSLVDIDGQKDEILIRARP 276

Query: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGK YD HELHAESLYFVHPITGIPLKLQ
Sbjct: 277 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQ 336

Query: 301 APLPSWASQALQPQQQEVNSP 322
           APLPSWASQA QPQQQEVNSP
Sbjct: 337 APLPSWASQAWQPQQQEVNSP 356

BLAST of Pay0019523 vs. NCBI nr
Match: XP_022156345.1 (RNA pseudouridine synthase 1 isoform X2 [Momordica charantia])

HSP 1 Score: 563.1 bits (1450), Expect = 1.5e-156
Identity = 279/330 (84.55%), Postives = 305/330 (92.42%), Query Frame = 0

Query: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60
           M D S PVTGN P+TG+NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANA-----GIKTNLPELHLANRLDRDTSGVMV 120
           EWLIAVNKPQG+YCE+VLA+VPRLL DSA A     GI+ +LPELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGIQISLPELHLANRLDRDTSGVMV 120

Query: 121 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 180
           ITKSHKVASKLVKAFTDH VSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 180

Query: 181 GRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVL 240
           GRSLPGGS+VRDMETYFEVLSVNG++TM+EL + RK+EEETIVV +KSL+DID+ KDE+L
Sbjct: 181 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 240

Query: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGI 300
           IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG  YDSHELHAESL+FVHP+TGI
Sbjct: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 300

Query: 301 PLKLQAPLPSWASQALQPQQQEVNSPQSFK 326
           PLKLQAPLPSWASQAL  QQQEV+SP + K
Sbjct: 301 PLKLQAPLPSWASQALLSQQQEVSSPSTLK 330

BLAST of Pay0019523 vs. NCBI nr
Match: XP_022156344.1 (RNA pseudouridine synthase 1 isoform X1 [Momordica charantia])

HSP 1 Score: 559.3 bits (1440), Expect = 2.2e-155
Identity = 279/340 (82.06%), Postives = 305/340 (89.71%), Query Frame = 0

Query: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60
           M D S PVTGN P+TG+NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAG---------------IKTNLPELHLANR 120
           EWLIAVNKPQG+YCE+VLA+VPRLL DSA AG               I+ +LPELHLANR
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGTTTLCLCLARIQISLPELHLANR 120

Query: 121 LDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFG 180
           LDRDTSGVMVITKSHKVASKLVKAFTDH VSKSYIAFCVG++PKWKKI+VKSGHGRSKFG
Sbjct: 121 LDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 180

Query: 181 VWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLV 240
           VWRVYAAADVGRSLPGGS+VRDMETYFEVLSVNG++TM+EL + RK+EEETIVV +KSL+
Sbjct: 181 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLI 240

Query: 241 DIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAES 300
           DID+ KDE+LIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG  YDSHELHAES
Sbjct: 241 DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES 300

Query: 301 LYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSPQSFK 326
           L+FVHP+TGIPLKLQAPLPSWASQAL  QQQEV+SP + K
Sbjct: 301 LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSPSTLK 340

BLAST of Pay0019523 vs. TAIR 10
Match: AT1G56345.1 (Pseudouridine synthase family protein )

HSP 1 Score: 361.7 bits (927), Expect = 6.3e-100
Identity = 181/294 (61.56%), Postives = 223/294 (75.85%), Query Frame = 0

Query: 18  NYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENV 77
           NYP P+S P P ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 78  LAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 137
           L + P+++ DS++        E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + K
Sbjct: 99  LRSAPQIVLDSSS--------EYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRK 158

Query: 138 SYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSV 197
           SYIA C+G+SP W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+SV
Sbjct: 159 SYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSV 218

Query: 198 NG-KNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDE--VLIRARPRSGRTHQIRLHCQY 257
           N  KN   EL+    +    IV   +  +      D+  V++RA PRSGRTHQIRLHCQY
Sbjct: 219 NSVKNESCELE----DVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQY 278

Query: 258 LGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWAS 309
           LGIPIRGDVKY GV EWNG+T++ HELHAE L   HP+TG  + ++APLP WA+
Sbjct: 279 LGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

BLAST of Pay0019523 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 72.0 bits (175), Expect = 9.9e-13
Identity = 73/304 (24.01%), Postives = 114/304 (37.50%), Query Frame = 0

Query: 55  DVIYEDEWLIAVNK------------PQGIYCENVLAAVPRLLCDSANA----------- 114
           D++YED+ ++ VNK            P G     +L        D +N+           
Sbjct: 149 DIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETF 208

Query: 115 ---GIKTNLPELHLA-------NRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIA 174
                 T  P  + A       +RLD+ T+G++V+ K     + L + F  HT+ + Y++
Sbjct: 209 SDDEEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVS 268

Query: 175 FCVGT-SPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGK 234
              G  SP   +I +  G   S     R+  AA     +PGG              V G 
Sbjct: 269 LTTGVPSPPQGRIEIPIGRDSSN----RIRMAA-----IPGG--------------VRGG 328

Query: 235 NTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIR 294
                  +++             +++  +     L+  R  +GRTHQIR H +Y+G+P+ 
Sbjct: 329 RARHAASRYK-------------VIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLL 388

Query: 295 GDVKYEG-------VTEWNGKTYDSHE------------LHAESLYFVHPITGIPLKLQA 306
           GD  Y G       + +      D  E            LHA  L F HP TG  +K   
Sbjct: 389 GDEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSC 416

BLAST of Pay0019523 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.3e-09
Identity = 76/279 (27.24%), Postives = 110/279 (39.43%), Query Frame = 0

Query: 14  STGENYPVPLS-----PPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNK 73
           + G+   +PLS     P  P   K      +   S +   F  S   V+Y+D  +I +NK
Sbjct: 145 NVGDRIYLPLSVDNDTPQTPPAKK-----ESFQCSDEERKFVCSL--VLYKDPAIIVLNK 204

Query: 74  PQGIYCENVLAAVPRLLCDSANAGIKTNLPEL-------------HLANRLDRDTSGVMV 133
           P G             L     +GIKT++ EL              L +RLDRD SG++V
Sbjct: 205 PHG-------------LAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLV 264

Query: 134 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 193
           + ++   A+ L   F + T          G S    K +VKS        + R Y A  +
Sbjct: 265 LARTQTAATVLHSIFREKT---------TGASAYGVKKNVKS--------LKRKYMALVI 324

Query: 194 G--------RSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDI 253
           G         S P   +V D +   E ++VN    +   Q             T+  V  
Sbjct: 325 GCPPRQRGQISAPLRKVVVD-DGKSERITVNDNGELVSTQH----------AITEYRVIE 375

Query: 254 DSQKDEVLIRARPRSGRTHQIRLHC-QYLGIPIRGDVKY 266
            S      +  RP +GR HQ+R+HC + LG PI GD KY
Sbjct: 385 SSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKY 375

BLAST of Pay0019523 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 55.8 bits (133), Expect = 7.4e-08
Identity = 58/223 (26.01%), Postives = 92/223 (41.26%), Query Frame = 0

Query: 55  DVIYEDEWLIAVNKPQGIYC--------ENVLAAVPRLLCDSANAGIKTNLPELHLANRL 114
           +V+YED+ LIA+NKP G+            VL  +      + +       P     +RL
Sbjct: 98  EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRL 157

Query: 115 DRDTSGVMVITKSHKVASKLVKAFTDHT--VSKSYIAFCVGTSPKWKKIHVKSGHGRSKF 174
            R TSG+++  K+    +KL   F + T  V    +    GT  K  KI+     G    
Sbjct: 158 GRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADG---- 217

Query: 175 GVWRVYAAADVGRSLPGGSIVR--DMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTK 234
               +    +V    P G +VR   +     V S  GK    ++                
Sbjct: 218 ----IVEEDEVVIKQPIG-VVRYPGVAQGLYVASPEGKPAFSKV---------------- 277

Query: 235 SLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKY 266
            +++ D +K+  L++   +SGR HQIR+H  Y+G P+ GD  Y
Sbjct: 278 FVLERDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY 295

BLAST of Pay0019523 vs. TAIR 10
Match: AT5G51140.1 (Pseudouridine synthase family protein )

HSP 1 Score: 52.4 bits (124), Expect = 8.1e-07
Identity = 52/196 (26.53%), Postives = 75/196 (38.27%), Query Frame = 0

Query: 96  NLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHV 155
           +L  L   +RLDR  SG+++I ++   A    +      V K YIA  +G  P+ + I  
Sbjct: 176 DLGTLFPIHRLDRLVSGLLIIARTAAKADFFRQQIEGGMVKKRYIAKVIGVFPEDEMI-- 235

Query: 156 KSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEE 215
                        V A  +   S  G S   D  +  +   V GK    +  +       
Sbjct: 236 -------------VDANINYNGS-EGRSTAEDANSSGDDKKVKGKPACTKFTR------- 295

Query: 216 TIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKT 275
                      ID+     L+   P +GRTHQIR+H QY G PI  D  Y      N +T
Sbjct: 296 -----------IDTNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLYLNQHIDNLET 337

Query: 276 YDSHELHAESLYFVHP 292
           Y +  + A     V P
Sbjct: 356 YIAKRIDAGERKIVSP 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2QNM38.5e-10261.00RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g05... [more]
Q7XA658.8e-9961.56RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 S... [more]
Q68XB22.6e-1831.25Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATC... [more]
Q87S653.8e-1727.74Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus sero... [more]
Q9ZDR73.2e-1628.13Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strai... [more]
Match NameE-valueIdentityDescription
A0A5A7TX255.8e-186100.00RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BW875.8e-186100.00RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1[more]
A0A0A0LJY62.2e-17795.43PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G03... [more]
A0A6J1DQ127.4e-15784.55RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1DT561.1e-15582.06RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_008453651.11.2e-185100.00PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudou... [more]
XP_004144814.34.6e-17795.43RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein ... [more]
XP_038889468.19.5e-16792.21RNA pseudouridine synthase 1 [Benincasa hispida][more]
XP_022156345.11.5e-15684.55RNA pseudouridine synthase 1 isoform X2 [Momordica charantia][more]
XP_022156344.12.2e-15582.06RNA pseudouridine synthase 1 isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G56345.16.3e-10061.56Pseudouridine synthase family protein [more]
AT1G76050.29.9e-1324.01Pseudouridine synthase family protein [more]
AT3G19440.11.3e-0927.24Pseudouridine synthase family protein [more]
AT3G52260.27.4e-0826.01Pseudouridine synthase family protein [more]
AT5G51140.18.1e-0726.53Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 51..304
e-value: 3.2E-46
score: 159.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR21600:SF47RNA PSEUDOURIDINE SYNTHASE 1coord: 30..315
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 30..315
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 63..253
e-value: 8.0E-25
score: 87.8
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 63..288
e-value: 1.08643E-40
score: 139.01
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 103..117
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 54..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0019523.1Pay0019523.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding