Pay0017130 (gene) Melon (Payzawat) v1

Overview


Name	Pay0017130
Type	gene
Organism	Cucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description	Cucumisin
Location	chr10: 7378697 .. 7382990 (+)
RNA-Seq Expression	Pay0017130
Synteny	Pay0017130

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATCAATATTATCCCCTCGTTAGTGGCCGTGATATACCCAATACTGGTTTCGATAGCTCCACCTCAAGGTTATGCACGAACAAGTCATTGAATCCCAGATTGTTAAAGGGAAAAATTGTTGTCTGTGAAACGAGTTTCGGTCCTATGGTATTCTTTAAGTCCTTGGATGGTGCAGCGGGTGTCCTCATGACATCAAATACGAGGGATTATGCACACTCCTATCCCTTGCCTTCTTCCATTCTCGACCCAAATGATCTCCTTGCCACTTTGCATTATATTTATTCAATTCGGTATGATATATACACATGTTACTGCTCTCATAATTAATTGGTTTTTGTTAGATAACATGCGTTTCCATTTTAAAACCAATTGAAAAGAAGACGAGTAGCTCATGTTGACAAATAAACGGTTCTTAGATAAGTATAACAGTTGTAACGACAGTTGTGCACAAACAATGAACCTAGAGTAACATGAAAGGAAACAAACAACACATTAACATTTGTTAACCTAGTTCGATGAATACACATCTATATCTAGGGATTTTATATATTGTGCATCTAATAATTGTTGAATACATAGTTATGACTGAAGGTATAACACTCTACACATTATTTATCATCGTGCTATTCTAAAACTGATTCATCCGAACTAAATATGTGAGAACATAATAGTTTTAACTCATAAGAAAGACATAATATCTCCCCCTTAATCTCAGATCGATGGATGATGTATTCAACTCAAGCCCTGAATGACTTGATGATTCTTCTATCGGTTTTTCGTATTATTTTCCATGAAGAACTACTACGAAAAACTCCTTCTCGTGCAAAAGCTTTATTTAGCTTTTCATTCTCAAACAAATTTTAACAAATCCTAACACCTATGAAACCATGCCTTGAAAAAGAAAATTGTTACCAATATACCTAAGGAATATAGATCCTAACTACCTCCTGTGGAACATCTTGAAAGAAAATGTTAACAGATTCTTTACAACTCCATAACCAATTAATTAATTAATTTACTCGTGATAAAATAAAGTAGGTGGGACCATTAACTAATACATGTATTTTATAAAGATTTTAAGTTTCCTTAATTGTTTCAACGTGGAATCACTCAACATGAAACTTCATGATGATGTCTTTTTTGGTTAACTATTTTTTAATTGACATCTACTAGTTTGTAGGCAAATGTATAGAAAAATTAAGGAACCTCACGATGTTATGTTACAAAATGATCGACAAGGAGTACAAAGAAACATAAAGACACATCGACAAACAAATATATGTGGTTTCATAATGGTGCATTAGCTATGTCCACGAACTATAGGGACAACAAATGAAGAACATAGGGTCATAATCATAAATAACCATTATAAATATTTACAAGCTAGACGTTATGCTTGGTTGTTTGTAGGCGTTAGATAACAAACTCAAAGCATTCCAAACATATTATAAAATAGATGTGAGGTGTTCTTCTCATTACCAATTCGTTATGAGATGGATAGACATTCTATCTGTACAACGGTGAACCCAACGAAAATACCATGCTGAGTATCCCACACATTGTAAAGGCAGCAAGACTTCACCATTATTGATTTTAACTTAATGTAGTAGCATATATGTTGAATTATTTATCAATATCGACATTGTTTAAACCTTTTTTTCTTATTAACTTCTGATGATTAACAGCTCTCCTGGTGCAACCATTTTCAAGAGTACCACAATCCTCAATGCATCTGCTCCTGTTGTTGTTTCCTTCTCATCTAGGGCACCTAATCGTGCAACTAAAGAAGTTATTAAGGTTAATTTTATTGAATTGAACTTTATGGTTACTATATTCTTAGTGTGGGTAAGATGGTGAATTAATTAGTTTAATTTCATGAACTTGAACTAATATTGCAGCGAAACATAAGTGGTCCAGGAGTTGAAATTCTAGCAGCATGGCCTCTTGTTGCCCCAGTTGGTGGAATTCGTAGAAGTACATTTTTTAATATAATCTCAGGAACCTCAATGTCTTGTCCACATATTACTTTTCTTCCATTAATCTTTCATTTTTGTTTGTCCGTTATCTTCTAGGTAAGGTCGTGGTGGTTTTTGAAACAAGTGTGTGTCTATACTATCACTATTATTAATAAAAAAAAATGTTATCTTTGCTCACGACCATTGATTATATTAGTTGTTTACGATTGTTAATGGTTATTCTTAGTAAAGAGGATTTCAAAAGTTTGTTTGAATGAATTTGTAAATTTACATCTTTAAAAGATTAGGATTATATGCCTTCTAATCCCAAAATGCACCCTTTTATGTATGAACTGGATCTAGAGGTGTAGGAGTGAAATGATCATACCATGCATGAGAAATGACTACTAAATAATTTAAAAGATGCATACTAAATAATTTAAAAGATGCACATTGTGAGTAATCTCCACAAATAAGAGAATGTCTTGCCAACTATCTTCTTGAAGAAAATGTACTTGGAGATATTTCTTTGAGTATGTGGGTATGCCTAAAAGCATTGTATGCGTATATACATACATACATACATACATACATACATACATATATATGTATGTATGTATATATAGGTATGTATGTATATGTATATGTATATGTATATTTATATATAAGTATACATATATTCTAGGATTGATTTTATTGTTGCTTGAATGGGAAAATGATTGAGTCCTAAGGAATACATGGAACTTAGTAAAACCAAAATAGAATCACTTTGTATTCTACATGTTATGATTCTTCATTCTTCGTTGTGTGATCTAGAATGTAATGTCCTATCCATGTCAATCTTGGATGAGGATGCCTCATAGTTACTGTGTGAACAGGTATGAGATGCCCTTTGCTTGCTATGTGATCTGGCAATGGAAGACCCTTAATGGGTAGTGAAATTAACAAAGCTTGCTAAAAGTGTTGAGTTTACTCCGCATAGCTATAACTTGGGAAAAGAGTTTGATAAATGTTTGATCCAAGGAAAGGGTACAAAGACTTAATTTGACTTACGGAAGGATTCTGCGGGTAGACTATGTTGTAAATTGCATGTTATAATGCTGCCTAGCTCCCCTTGAATAATCCCCTATTCTAAATTATTTGAATTCATTAGATAAAAGATCCCAGTCGTTAGACAATTTTTCCTAACCTTTTAAATGTTTGTCTTATTTCCCTCCAAGTAGTAAACGACATTCGATGTGGAACTTGCTATCTTGATCACCTATCGTTCCTCCTTAATCACATCATTTTGGTAATGGCCACTATTATCTCGCGTTTAGTATAGAAGCTAGGCGAGAAGAGTTAAGTGTTGTTAAGTTGCATTATGTTCTCTAAAGAGAAACTTCGTATTAAACATCAAATGTATTCACGTATTTTGAAAATTAATATATGAAGTTGCCTCCTTTTGCACCTGCTCGTGTCCCATGTCTAAATTTTATTGGTTGTACTTATTTTCGCTTACTAGAAGTTCAACGTTTACCTCATGGGAGTTACGCGTTAAGGGTCTAGGTGACACACCTCTCCTTAGTCACAAAAAGTGACTTAGGACGGGGCATGACACATTATGACATGCATAGGCATAATGTACAAATTATAACATGATATAGGTAGTGAACTTGAACAAATACAAAACATAGTATAAGTAGTGATCCTGAAAGAACAGACTGATACTAAGTTAGATTAGTCATTCCTTAAATTCTTATAATCTTGCCAACTCCTACTTAACTAAATTCTAAGTCTTCACATGTTACAACACCCAAAATCCACTCCACTAATCTAATAGTTATAACCTCGAGAACCTCTTTAAACATCCTACACGATCGCTGACCCTTCTAAATGATCGTTTAATCACATACACGATCATTTAGCCTTTTTATATGATCGTTTAACTTTTCAAATGATCATTTACTTCTTATATTGTTGTCTAACATTCTTACATGATTGTTTATCTAGCATTCTTACACGATCGTTTGCTTTCTTATACGATCATTTAGCAAAACACTTGTCAAAATCTACTATTCAAGCAAGGAACTTACAACTCTCTCCACCTTTATAAATTTTGTCCTCGAAATTTAAATAATAGTTCTTAACTTCAAACTTTTTATAAACAACTTTATTGCATAAAATTTATTCATTACGTGGTATTTACCTGATGAAACAAAAGTAAATTCAACAGACAATACAACTTTGCTATTCCAAAATTCTCTTTTCCTCACTGTCTTATAAATTCTATATTTACAACCCTTTTTTTCCTTTTTCCTTTATGTAAGGCAAAACCTCCCCTTGATGGTTGCTGA

mRNA sequence

ATGAATCAATATTATCCCCTCGTTAGTGGCCGTGATATACCCAATACTGGTTTCGATAGCTCCACCTCAAGGTTATGCACGAACAAGTCATTGAATCCCAGATTGTTAAAGGGAAAAATTGTTGTCTGTGAAACGAGTTTCGGTCCTATGGTATTCTTTAAGTCCTTGGATGGTGCAGCGGGTGTCCTCATGACATCAAATACGAGGGATTATGCACACTCCTATCCCTTGCCTTCTTCCATTCTCGACCCAAATGATCTCCTTGCCACTTTGCATTATATTTATTCAATTCGCTCTCCTGGTGCAACCATTTTCAAGAGTACCACAATCCTCAATGCATCTGCTCCTGTTGTTGTTTCCTTCTCATCTAGGGCACCTAATCGTGCAACTAAAGAAGTTATTAAGGCAAAACCTCCCCTTGATGGTTGCTGA

Coding sequence (CDS)

ATGAATCAATATTATCCCCTCGTTAGTGGCCGTGATATACCCAATACTGGTTTCGATAGCTCCACCTCAAGGTTATGCACGAACAAGTCATTGAATCCCAGATTGTTAAAGGGAAAAATTGTTGTCTGTGAAACGAGTTTCGGTCCTATGGTATTCTTTAAGTCCTTGGATGGTGCAGCGGGTGTCCTCATGACATCAAATACGAGGGATTATGCACACTCCTATCCCTTGCCTTCTTCCATTCTCGACCCAAATGATCTCCTTGCCACTTTGCATTATATTTATTCAATTCGCTCTCCTGGTGCAACCATTTTCAAGAGTACCACAATCCTCAATGCATCTGCTCCTGTTGTTGTTTCCTTCTCATCTAGGGCACCTAATCGTGCAACTAAAGAAGTTATTAAGGCAAAACCTCCCCTTGATGGTTGCTGA

Protein sequence

MNQYYPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLMTSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSSRAPNRATKEVIKAKPPLDGC

Homology

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BLAST of Pay0017130 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 6.9e-64
Identity = 123/141 (87.23%), Postives = 128/141 (90.78%), Query Frame = 0


Query: 2   NQYYPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAG 61
           NQYYPLVSGRDIPNTGFD STSR CT+KS+NP LLKGKIVVCE SFGP  FFKSLDGAAG
Sbjct: 356 NQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAG 415

Query: 62  VLMTSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSF 121
           VLMTSNTRDYA SYPLPSS+LDPNDLLATL YIYSIRSPGATIFKSTTILNASAPVVVSF
Sbjct: 416 VLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSF 475

Query: 122 SSRAPNRATKEVIKAKPPLDG 143
           SSR PNRATK+VI  KP + G
Sbjct: 476 SSRGPNRATKDVI--KPDISG 494

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BLAST of Pay0017130 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 7.0e-16
Identity = 50/131 (38.17%), Postives = 74/131 (56.49%), Query Frame = 0


Query: 5   YPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLM 64
           YPLV G+   ++  D+ T+ LC    LN   +KGKI+VC    G  +  KS+ GA  ++ 
Sbjct: 354 YPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKI-AKSV-GAIAIID 413

Query: 65  TSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 124
            S   D A ++ LP+S L   D  + + YI S  SP A + K+ TI N ++PV+ SFSSR
Sbjct: 414 KSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSR 473

Query: 125 APNRATKEVIK 136
            PN    +++K
Sbjct: 474 GPNTIAVDILK 482

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BLAST of Pay0017130 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.7e-14
Identity = 46/131 (35.11%), Postives = 72/131 (54.96%), Query Frame = 0


Query: 5   YPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLM 64
           YPLV G+   ++  D+S++  C+   L+ + +KGKIV+C+T   P        GA   ++
Sbjct: 363 YPLVYGKS-ASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGE--AQAMGAVASIV 422

Query: 65  TSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 124
            +   D A  +  P S+L  +D    L Y+ S ++P A + KS TI N  APVV S+SSR
Sbjct: 423 RNPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSR 482

Query: 125 APNRATKEVIK 136
            PN    +++K
Sbjct: 483 GPNPLIHDILK 490

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BLAST of Pay0017130 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.1e-13
Identity = 44/131 (33.59%), Postives = 74/131 (56.49%), Query Frame = 0


Query: 5   YPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLM 64
           YPLV G+   ++  D+ ++ LC    ++   +KGKI+VC    G +   +S+ GA G++ 
Sbjct: 353 YPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGP-GGLKIVESV-GAVGLIY 412

Query: 65  TSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 124
            +   D A  +PLP++ L   D  + + Y+ S  SP A + K+  I N ++PV+ SFSSR
Sbjct: 413 RTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSR 472

Query: 125 APNRATKEVIK 136
            PN    +++K
Sbjct: 473 GPNTIAVDILK 481

[Close]

BLAST of Pay0017130 vs. ExPASy Swiss-Prot
Match: F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.1e-12
Identity = 43/131 (32.82%), Postives = 71/131 (54.20%), Query Frame = 0


Query: 5   YPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLM 64
           YPLV G+   ++   ++++  C+   L+ + +KGKIV+C++   P        GA   ++
Sbjct: 363 YPLVYGKS-ASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDE--AQAMGAIASIV 422

Query: 65  TSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 124
            S+  D A  +  P S+L  +D    L Y+ S ++P A + KS TI N  APVV S+ SR
Sbjct: 423 RSHRTDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSR 482

Query: 125 APNRATKEVIK 136
            PN    +++K
Sbjct: 483 GPNTIIPDILK 490

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BLAST of Pay0017130 vs. ExPASy TrEMBL
Match: A0A1S3CF86 (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.8e-59
Identity = 120/141 (85.11%), Postives = 126/141 (89.36%), Query Frame = 0


Query: 2   NQYYPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAG 61
           NQYYPLVSGRDIPN GFD STSR CT+ S+ P+LLKGKIVVCE SFGP  FFKSLDGAAG
Sbjct: 356 NQYYPLVSGRDIPNPGFDKSTSRFCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAG 415

Query: 62  VLMTSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSF 121
           VLMTSNTRDYA SYPLPSS+LDPNDLLATL YIYSIRSPGATIFKSTTILNASAPVVVSF
Sbjct: 416 VLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSF 475

Query: 122 SSRAPNRATKEVIKAKPPLDG 143
           SSR PNRATK+VI  KP + G
Sbjct: 476 SSRGPNRATKDVI--KPDISG 494

[Close]

BLAST of Pay0017130 vs. ExPASy TrEMBL
Match: A0A5D3E3U6 (Cucumisin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold267G00160 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 2.1e-47
Identity = 101/120 (84.17%), Postives = 107/120 (89.17%), Query Frame = 0


Query: 23  SRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLMTSNTRDYAHSYPLPSSIL 82
           S  CT+ S+NP+LLKGKIVVCE SFGP  FFKSLDGAAGVLMTSNTRDYA SYPLPSS+L
Sbjct: 324 SAFCTDNSVNPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVL 383

Query: 83  DPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSSRAPNRATKEVIKAKPPLDG 142
           DPNDLLATL YIYSIRSPGATIFKSTTILNASAPVVVSFSSR PNRATK+VI  KP + G
Sbjct: 384 DPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVI--KPDISG 441

[Close]

BLAST of Pay0017130 vs. ExPASy TrEMBL
Match: A0A5A7UDR3 (Cucumisin OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001700 PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.7e-45
Identity = 101/126 (80.16%), Postives = 108/126 (85.71%), Query Frame = 0


Query: 18  FDSSTSRLCTNKSL-NPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLMTSNTRDYAHSYP 77
           F S+ + L  +  L NP+LLKGKIVVCE SFGP  FFKSLDGAAGVLMTSNTRDYA SYP
Sbjct: 322 FHSACASLVLHGQLSNPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYP 381

Query: 78  LPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSSRAPNRATKEVIKA 137
           LPSS+LDPNDLLATL YIYSIRSPGATIFKSTTILNASAPVVVSFSSR PNRATK+VI  
Sbjct: 382 LPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVI-- 441

Query: 138 KPPLDG 143
           KP + G
Sbjct: 442 KPDISG 445

[Close]

BLAST of Pay0017130 vs. ExPASy TrEMBL
Match: A0A5A7UD73 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001640 PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 5.5e-32
Identity = 81/139 (58.27%), Postives = 97/139 (69.78%), Query Frame = 0


Query: 5   YPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLM 64
           YPLV+GR +PNTGFDS+ S  C N S++ +L+KGKI++CE +F     F +L G AGVLM
Sbjct: 351 YPLVTGRQVPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDAK-HFVTLGGVAGVLM 410

Query: 65  -TSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSS 124
             +   D A SYP+PS+ILD ND +AT  YIYS  SP ATIFKST   N  APVVVSFSS
Sbjct: 411 IDTELIDNARSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSS 470

Query: 125 RAPNRATKEVIKAKPPLDG 143
           R PN  TKE+I  KP L G
Sbjct: 471 RGPNNITKEII--KPDLSG 486

[Close]

BLAST of Pay0017130 vs. ExPASy TrEMBL
Match: K7NBW1 (Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 5.5e-32
Identity = 74/135 (54.81%), Postives = 97/135 (71.85%), Query Frame = 0


Query: 1   MNQYYPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAA 60
           M   YPL+   + P+ GF+SSTSR C   S++P L++GKI++C+++FGP V F S  GAA
Sbjct: 358 MRNQYPLIYAGNAPSIGFNSSTSRYCYEDSVDPNLVRGKILLCDSTFGPTV-FASFGGAA 417

Query: 61  GVLMTSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVS 120
           GVLM SNTRD+A SYPLP+S+LDP        Y+ S R+P ATIFKST + + SAPVVVS
Sbjct: 418 GVLMQSNTRDHASSYPLPASVLDPAGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVS 477

Query: 121 FSSRAPNRATKEVIK 136
           FSSR PN  T +++K
Sbjct: 478 FSSRGPNYVTHDILK 491

[Close]

BLAST of Pay0017130 vs. NCBI nr
Match: 3VTA_A (Crystal Structure of cucumisin, a subtilisin-like endoprotease from Cucumis melo L [Cucumis melo] >3VTA_B Crystal Structure of cucumisin, a subtilisin-like endoprotease from Cucumis melo L [Cucumis melo] >4YN3_A Crystal structure of Cucumisin complex with pro-peptide [Cucumis melo])

HSP 1 Score: 244.6 bits (623), Expect = 5.2e-61
Identity = 123/141 (87.23%), Postives = 128/141 (90.78%), Query Frame = 0


Query: 2   NQYYPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAG 61
           NQYYPLVSGRDIPNTGFD STSR CT+KS+NP LLKGKIVVCE SFGP  FFKSLDGAAG
Sbjct: 246 NQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAG 305

Query: 62  VLMTSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSF 121
           VLMTSNTRDYA SYPLPSS+LDPNDLLATL YIYSIRSPGATIFKSTTILNASAPVVVSF
Sbjct: 306 VLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSF 365

Query: 122 SSRAPNRATKEVIKAKPPLDG 143
           SSR PNRATK+VI  KP + G
Sbjct: 366 SSRGPNRATKDVI--KPDISG 384

[Close]

BLAST of Pay0017130 vs. NCBI nr
Match: Q39547.1 (RecName: Full=Cucumisin; AltName: Allergen=Cuc m 1; Flags: Precursor [Cucumis melo] >BAA06905.1 pre-pro-cucumisin [Cucumis melo])

HSP 1 Score: 244.6 bits (623), Expect = 5.2e-61
Identity = 123/141 (87.23%), Postives = 128/141 (90.78%), Query Frame = 0


Query: 2   NQYYPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAG 61
           NQYYPLVSGRDIPNTGFD STSR CT+KS+NP LLKGKIVVCE SFGP  FFKSLDGAAG
Sbjct: 356 NQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAG 415

Query: 62  VLMTSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSF 121
           VLMTSNTRDYA SYPLPSS+LDPNDLLATL YIYSIRSPGATIFKSTTILNASAPVVVSF
Sbjct: 416 VLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSF 475

Query: 122 SSRAPNRATKEVIKAKPPLDG 143
           SSR PNRATK+VI  KP + G
Sbjct: 476 SSRGPNRATKDVI--KPDISG 494

[Close]

BLAST of Pay0017130 vs. NCBI nr
Match: XP_008461721.1 (PREDICTED: LOW QUALITY PROTEIN: cucumisin [Cucumis melo])

HSP 1 Score: 238.4 bits (607), Expect = 3.8e-59
Identity = 120/141 (85.11%), Postives = 126/141 (89.36%), Query Frame = 0


Query: 2   NQYYPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAG 61
           NQYYPLVSGRDIPN GFD STSR CT+ S+ P+LLKGKIVVCE SFGP  FFKSLDGAAG
Sbjct: 356 NQYYPLVSGRDIPNPGFDKSTSRFCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAG 415

Query: 62  VLMTSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSF 121
           VLMTSNTRDYA SYPLPSS+LDPNDLLATL YIYSIRSPGATIFKSTTILNASAPVVVSF
Sbjct: 416 VLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSF 475

Query: 122 SSRAPNRATKEVIKAKPPLDG 143
           SSR PNRATK+VI  KP + G
Sbjct: 476 SSRGPNRATKDVI--KPDISG 494

[Close]

BLAST of Pay0017130 vs. NCBI nr
Match: TYK30793.1 (cucumisin [Cucumis melo var. makuwa])

HSP 1 Score: 198.4 bits (503), Expect = 4.3e-47
Identity = 101/120 (84.17%), Postives = 107/120 (89.17%), Query Frame = 0


Query: 23  SRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLMTSNTRDYAHSYPLPSSIL 82
           S  CT+ S+NP+LLKGKIVVCE SFGP  FFKSLDGAAGVLMTSNTRDYA SYPLPSS+L
Sbjct: 324 SAFCTDNSVNPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVL 383

Query: 83  DPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSSRAPNRATKEVIKAKPPLDG 142
           DPNDLLATL YIYSIRSPGATIFKSTTILNASAPVVVSFSSR PNRATK+VI  KP + G
Sbjct: 384 DPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVI--KPDISG 441

[Close]

BLAST of Pay0017130 vs. NCBI nr
Match: XP_038892506.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 196.4 bits (498), Expect = 1.6e-46
Identity = 99/135 (73.33%), Postives = 107/135 (79.26%), Query Frame = 0


Query: 1   MNQYYPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAA 60
           MN  YPLV GRDIPN GFDSSTSR C NKS+NP LL+GKIV+CE SFGP  FFKSLDGAA
Sbjct: 361 MNGQYPLVLGRDIPNIGFDSSTSRYCFNKSVNPYLLRGKIVLCEASFGPAEFFKSLDGAA 420

Query: 61  GVLMTSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVS 120
           GVLM ++TRD+A SYP PSS LDPND      YIYS   P ATIFKST ILN SAPVVVS
Sbjct: 421 GVLMLASTRDHASSYPFPSSTLDPNDATDIFRYIYSTSFPTATIFKSTAILNTSAPVVVS 480

Query: 121 FSSRAPNRATKEVIK 136
           FSSR PN ATK++IK
Sbjct: 481 FSSRGPNAATKDLIK 495

[Close]

BLAST of Pay0017130 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 85.1 bits (209), Expect = 4.9e-17
Identity = 50/131 (38.17%), Postives = 74/131 (56.49%), Query Frame = 0


Query: 5   YPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLM 64
           YPLV G+   ++  D+ T+ LC    LN   +KGKI+VC    G  +  KS+ GA  ++ 
Sbjct: 354 YPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKI-AKSV-GAIAIID 413

Query: 65  TSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 124
            S   D A ++ LP+S L   D  + + YI S  SP A + K+ TI N ++PV+ SFSSR
Sbjct: 414 KSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSR 473

Query: 125 APNRATKEVIK 136
            PN    +++K
Sbjct: 474 GPNTIAVDILK 482

[Close]

BLAST of Pay0017130 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 85.1 bits (209), Expect = 4.9e-17
Identity = 50/131 (38.17%), Postives = 74/131 (56.49%), Query Frame = 0


Query: 5   YPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLM 64
           YPLV G+   ++  D+ T+ LC    LN   +KGKI+VC    G  +  KS+ GA  ++ 
Sbjct: 354 YPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKI-AKSV-GAIAIID 413

Query: 65  TSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 124
            S   D A ++ LP+S L   D  + + YI S  SP A + K+ TI N ++PV+ SFSSR
Sbjct: 414 KSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSR 473

Query: 125 APNRATKEVIK 136
            PN    +++K
Sbjct: 474 GPNTIAVDILK 482

[Close]

BLAST of Pay0017130 vs. TAIR 10
Match: AT5G59090.3 (subtilase 4.12 )

HSP 1 Score: 85.1 bits (209), Expect = 4.9e-17
Identity = 50/131 (38.17%), Postives = 74/131 (56.49%), Query Frame = 0


Query: 5   YPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLM 64
           YPLV G+   ++  D+ T+ LC    LN   +KGKI+VC    G  +  KS+ GA  ++ 
Sbjct: 352 YPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKI-AKSV-GAIAIID 411

Query: 65  TSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 124
            S   D A ++ LP+S L   D  + + YI S  SP A + K+ TI N ++PV+ SFSSR
Sbjct: 412 KSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSR 471

Query: 125 APNRATKEVIK 136
            PN    +++K
Sbjct: 472 GPNTIAVDILK 480

[Close]

BLAST of Pay0017130 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.2e-15
Identity = 46/131 (35.11%), Postives = 72/131 (54.96%), Query Frame = 0


Query: 5   YPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLM 64
           YPLV G+   ++  D+S++  C+   L+ + +KGKIV+C+T   P        GA   ++
Sbjct: 363 YPLVYGKS-ASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGE--AQAMGAVASIV 422

Query: 65  TSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 124
            +   D A  +  P S+L  +D    L Y+ S ++P A + KS TI N  APVV S+SSR
Sbjct: 423 RNPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSR 482

Query: 125 APNRATKEVIK 136
            PN    +++K
Sbjct: 483 GPNPLIHDILK 490

[Close]

BLAST of Pay0017130 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 77.8 bits (190), Expect = 7.9e-15
Identity = 44/131 (33.59%), Postives = 74/131 (56.49%), Query Frame = 0


Query: 5   YPLVSGRDIPNTGFDSSTSRLCTNKSLNPRLLKGKIVVCETSFGPMVFFKSLDGAAGVLM 64
           YPLV G+   ++  D+ ++ LC    ++   +KGKI+VC    G +   +S+ GA G++ 
Sbjct: 353 YPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGP-GGLKIVESV-GAVGLIY 412

Query: 65  TSNTRDYAHSYPLPSSILDPNDLLATLHYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 124
            +   D A  +PLP++ L   D  + + Y+ S  SP A + K+  I N ++PV+ SFSSR
Sbjct: 413 RTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSR 472

Query: 125 APNRATKEVIK 136
            PN    +++K
Sbjct: 473 GPNTIAVDILK 481

The following BLAST results are available for this feature:

BLAST of Pay0017130 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39547	6.9e-64	87.23	Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q8L7D2	7.0e-16	38.17	Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2...	[more]
Q9STF7	1.7e-14	35.11	Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S...	[more]
Q9FIG2	1.1e-13	33.59	Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2...	[more]
F4JA91	2.1e-12	32.82	Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 S...	[more]

BLAST of Pay0017130 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CF86	1.8e-59	85.11	LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1	[more]
A0A5D3E3U6	2.1e-47	84.17	Cucumisin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold267G00160 PE=3...	[more]
A0A5A7UDR3	5.7e-45	80.16	Cucumisin OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001700 PE=...	[more]
A0A5A7UD73	5.5e-32	58.27	Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G00164...	[more]
K7NBW1	5.5e-32	54.81	Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]

BLAST of Pay0017130 vs. NCBI nr


Match Name	E-value	Identity	Description
3VTA_A	5.2e-61	87.23	Crystal Structure of cucumisin, a subtilisin-like endoprotease from Cucumis melo...	[more]
Q39547.1	5.2e-61	87.23	RecName: Full=Cucumisin; AltName: Allergen=Cuc m 1; Flags: Precursor [Cucumis me...	[more]
XP_008461721.1	3.8e-59	85.11	PREDICTED: LOW QUALITY PROTEIN: cucumisin [Cucumis melo]	[more]
TYK30793.1	4.3e-47	84.17	cucumisin [Cucumis melo var. makuwa]	[more]
XP_038892506.1	1.6e-46	73.33	cucumisin-like [Benincasa hispida]	[more]

BLAST of Pay0017130 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59090.1	4.9e-17	38.17	subtilase 4.12	[more]
AT5G59090.2	4.9e-17	38.17	subtilase 4.12	[more]
AT5G59090.3	4.9e-17	38.17	subtilase 4.12	[more]
AT3G46850.1	1.2e-15	35.11	Subtilase family protein	[more]
AT5G59120.1	7.9e-15	33.59	subtilase 4.13	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.50.30.30		coord: 2..111 e-value: 2.3E-34 score: 121.0
None	No IPR available	PANTHER	PTHR10795:SF685	SUBTILISIN-LIKE PROTEASE SBT4.3	coord: 3..136
None	No IPR available	CDD	cd02120	PA_subtilisin_like	coord: 2..104 e-value: 1.08597E-9 score: 50.8751
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 112..135 e-value: 2.3E-34 score: 121.0
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 3..136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Pay0017130.1	Pay0017130.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004252	serine-type endopeptidase activity