Pay0016469 (gene) Melon (Payzawat) v1

Overview
NamePay0016469
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionB3 domain-containing protein
Locationchr08: 23175734 .. 23176917 (+)
RNA-Seq ExpressionPay0016469
SyntenyPay0016469
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCGTCAAACAAAATCTAAAATATAAACTTAAAAAGTCGAAAAAGGGGGAAAGAAGGATTTAAATCTAAAATTGATTAAGAGAAAAAGAAGGGTCAATCCTATTATTCTCTAAAACCCCAAAATCTCAAGAACCCCCATTCTTCTTATCTTCTTCCTCCTTTCCGCCGGTGAAATTATGGAAATGGATTCCAACACTTCAAGTCCCTCCGCCAACGCCCAACAAGAACAAGAAACCACAGTCTCTACAAATGGAGAAATCACCGATCAAAACCCCAGCGACGACGACGACCATCTTCCACTAATAAAGAGAAAGAGAACCAGAATGAAACAACTCGCAAGGCGACACAAACGCGATAGAGAAAAAATACTAAAGCGCCCACTCAAACACCCAGTCAACGATCCAATTCCCAAATCCGACGAAGAACCACAAGGCAAGGACTTGGTATTCGACACAGAAACCGAAAGCGAGGGCGATGCAGATCAAAATCAAGAAACCCTAACATTTATGGCTTTAGCAGAAATCGTGGAATCAGAAAGAGAATTCCTCGAAAACATACCTGAAGAATACAAGAAACTGATGATTTTCATGGGAGGATCGTGGGATGTTTGCCCTAGTTTGGTGATGGAGAAGCGACTGAAGAAAGGGGATGTGGAGGAACGAAGAAATCGGCTGTTGATTCCACAAAGGAAGACGAGAACGACATTTCTGGAAGTTGAAGAAGAGGAGAAGTTGAACACAGATGTTTGGTGGATGGTGGAGATTATTGAGCCGGATTGTACGGTGAGTTTGATTACGCTGAGTAAATGGGAAACGCGGAAGGGAGTGGCGTATGTTTTGATAACGGAGTGGAATGGATTGGTGGAGAGGAATGACTTGAAGGAAGGTGATTTGATGCAGCTATGGAGTTTTCGTGCCGGCGGCGGCCGTGGACGGCTCTGTTTTATGCTTCTGGAGGTGGAAGAAAACCGAGAAAGACGGTAGTGAAATTGCTTCCGCTCAATCTTAGGAGATTTTTTTTTTGCCCTTCTGGAGGGGTATTTTGGTAATTTTGTTTTGTTTTGTCTTGTTTTTTTTTTTTTTAAAAGACTATTGGTGTTTGTTTTCAATGAAACTATTTGTACATAATAAATACATTTGATACTTTTGTTCTAAGCTAAAACTAAAATGGATATAGTCGAGCAT

mRNA sequence

GGCGTCAAACAAAATCTAAAATATAAACTTAAAAAGTCGAAAAAGGGGGAAAGAAGGATTTAAATCTAAAATTGATTAAGAGAAAAAGAAGGGTCAATCCTATTATTCTCTAAAACCCCAAAATCTCAAGAACCCCCATTCTTCTTATCTTCTTCCTCCTTTCCGCCGGTGAAATTATGGAAATGGATTCCAACACTTCAAGTCCCTCCGCCAACGCCCAACAAGAACAAGAAACCACAGTCTCTACAAATGGAGAAATCACCGATCAAAACCCCAGCGACGACGACGACCATCTTCCACTAATAAAGAGAAAGAGAACCAGAATGAAACAACTCGCAAGGCGACACAAACGCGATAGAGAAAAAATACTAAAGCGCCCACTCAAACACCCAGTCAACGATCCAATTCCCAAATCCGACGAAGAACCACAAGGCAAGGACTTGGTATTCGACACAGAAACCGAAAGCGAGGGCGATGCAGATCAAAATCAAGAAACCCTAACATTTATGGCTTTAGCAGAAATCGTGGAATCAGAAAGAGAATTCCTCGAAAACATACCTGAAGAATACAAGAAACTGATGATTTTCATGGGAGGATCGTGGGATGTTTGCCCTAGTTTGGTGATGGAGAAGCGACTGAAGAAAGGGGATGTGGAGGAACGAAGAAATCGGCTGTTGATTCCACAAAGGAAGACGAGAACGACATTTCTGGAAGTTGAAGAAGAGGAGAAGTTGAACACAGATGTTTGGTGGATGGTGGAGATTATTGAGCCGGATTGTACGGTGAGTTTGATTACGCTGAGTAAATGGGAAACGCGGAAGGGAGTGGCGTATGTTTTGATAACGGAGTGGAATGGATTGGTGGAGAGGAATGACTTGAAGGAAGGTGATTTGATGCAGCTATGGAGTTTTCGTGCCGGCGGCGGCCGTGGACGGCTCTGTTTTATGCTTCTGGAGGTGGAAGAAAACCGAGAAAGACGGTAGTGAAATTGCTTCCGCTCAATCTTAGGAGATTTTTTTTTTGCCCTTCTGGAGGGGTATTTTGGTAATTTTGTTTTGTTTTGTCTTGTTTTTTTTTTTTTTAAAAGACTATTGGTGTTTGTTTTCAATGAAACTATTTGTACATAATAAATACATTTGATACTTTTGTTCTAAGCTAAAACTAAAATGGATATAGTCGAGCAT

Coding sequence (CDS)

ATGGAAATGGATTCCAACACTTCAAGTCCCTCCGCCAACGCCCAACAAGAACAAGAAACCACAGTCTCTACAAATGGAGAAATCACCGATCAAAACCCCAGCGACGACGACGACCATCTTCCACTAATAAAGAGAAAGAGAACCAGAATGAAACAACTCGCAAGGCGACACAAACGCGATAGAGAAAAAATACTAAAGCGCCCACTCAAACACCCAGTCAACGATCCAATTCCCAAATCCGACGAAGAACCACAAGGCAAGGACTTGGTATTCGACACAGAAACCGAAAGCGAGGGCGATGCAGATCAAAATCAAGAAACCCTAACATTTATGGCTTTAGCAGAAATCGTGGAATCAGAAAGAGAATTCCTCGAAAACATACCTGAAGAATACAAGAAACTGATGATTTTCATGGGAGGATCGTGGGATGTTTGCCCTAGTTTGGTGATGGAGAAGCGACTGAAGAAAGGGGATGTGGAGGAACGAAGAAATCGGCTGTTGATTCCACAAAGGAAGACGAGAACGACATTTCTGGAAGTTGAAGAAGAGGAGAAGTTGAACACAGATGTTTGGTGGATGGTGGAGATTATTGAGCCGGATTGTACGGTGAGTTTGATTACGCTGAGTAAATGGGAAACGCGGAAGGGAGTGGCGTATGTTTTGATAACGGAGTGGAATGGATTGGTGGAGAGGAATGACTTGAAGGAAGGTGATTTGATGCAGCTATGGAGTTTTCGTGCCGGCGGCGGCCGTGGACGGCTCTGTTTTATGCTTCTGGAGGTGGAAGAAAACCGAGAAAGACGGTAG

Protein sequence

MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRDREKILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESEREFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEVEEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLMQLWSFRAGGGRGRLCFMLLEVEENRERR
Homology
BLAST of Pay0016469 vs. ExPASy Swiss-Prot
Match: Q9FLV7 (B3 domain-containing protein At5g24050 OS=Arabidopsis thaliana OX=3702 GN=At5g24050 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 7.7e-08
Identity = 48/143 (33.57%), Postives = 66/143 (46.15%), Query Frame = 0

Query: 128 PEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIP-QRKTRTTFLE------V 187
           PE   K+M  M  + D    L+ EK L   DV   +NRL +P     R  FL       +
Sbjct: 200 PEWLVKVMTNMKEAKDA--KLIFEKTLFVTDVNPTKNRLSMPFNNLLRNDFLTSVESIII 259

Query: 188 EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRK-----GVAYVLITEWNGLVERNDLK 247
            E+   N  +     +++  C    + L +WE +K       +Y LI  WN +VE N LK
Sbjct: 260 NEDINNNNKIGVGAILVDQRCKKWGVMLKRWEMKKESGKGSWSYNLICGWNDIVEANRLK 319

Query: 248 EGDLMQLWSFRAGGGRGRLCFML 259
           EGD + LWSFR     G LCF +
Sbjct: 320 EGDNITLWSFRC---CGILCFAM 337

BLAST of Pay0016469 vs. ExPASy Swiss-Prot
Match: Q9LSF7 (B3 domain-containing protein At3g25182 OS=Arabidopsis thaliana OX=3702 GN=At3g25182 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 6.5e-07
Identity = 43/123 (34.96%), Postives = 59/123 (47.97%), Query Frame = 0

Query: 148 LVMEKRLKKGDVEERRNRLLIP-QRKTRTTFLEVEEEEKLNTDVWWMVEI------IEPD 207
           L+ EK L   DV   +NRL +P     R  FL   E   ++ D+    +I      ++  
Sbjct: 215 LIFEKTLFVTDVNPTQNRLSMPFNNLLRNDFLTSVESRIIDKDIKNDKKIGVGAILVDQR 274

Query: 208 CTVSLITLSKWETRKGVA-----YVLITEWNGLVERNDLKEGDLMQLWSFRAGGGRGRLC 259
           C    + L +WE +K        Y LI  WN +VE N LKEGD + LWSFR+    G LC
Sbjct: 275 CKKWGVMLKRWEMKKESGKGSWNYNLICGWNDIVEANGLKEGDNISLWSFRS---CGILC 334

BLAST of Pay0016469 vs. ExPASy Swiss-Prot
Match: Q5RM09 (B3 domain-containing protein At1g05920 OS=Arabidopsis thaliana OX=3702 GN=At1g05920 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 4.2e-06
Identity = 69/242 (28.51%), Postives = 109/242 (45.04%), Query Frame = 0

Query: 47  RTRMKQLARRHKRDREKIL-------KRPLKHPVNDPIPK----SDEEPQGKDLVFDTET 106
           RT+ K +A+     R++         + P  +  NDP P+        P+ KD++ D   
Sbjct: 82  RTKPKTVAQVRDAKRKRTFDHVPRGTREPHAYLRNDPAPQVASVPKSVPEEKDVILD--- 141

Query: 107 ESEGDADQNQETLTFMALAEIVESEREFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLK 166
               +  + ++T ++    E V  +       P+   ++M  M G+ D  P L++EK L 
Sbjct: 142 RILSNVPRRKKTTSY----EFVPPKH---PQEPQWLLQVMSRMNGAGD--PKLIIEKNLD 201

Query: 167 KGDVEERRNRLLIP-QRKTRTTFLEVEEEEKLNTDVWWMVEII-EPDCTVSLITL----S 226
             DV+ R+NRL IP     +  FL ++E   ++ D     EI  E +  V+   +     
Sbjct: 202 SNDVDPRQNRLSIPINTVIQNDFLTLDESRLIDED-----EITNEGNMGVAAFLVDQRTK 261

Query: 227 KWE---------TRKGVAY---VLITEWNGLVERNDLKEGDLMQLWSFRAGGGRGRLCFM 260
           KW          T  G +Y   VL  EW+ +VE N LKEGD + LWSFR+      LCF 
Sbjct: 262 KWNMGFKQWFMTTDSGSSYWSFVLRGEWSNVVETNGLKEGDKISLWSFRS---NDILCFA 303

BLAST of Pay0016469 vs. ExPASy Swiss-Prot
Match: Q9LRX6 (Putative B3 domain-containing protein At3g24850 OS=Arabidopsis thaliana OX=3702 GN=At3g24850 PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 9.4e-06
Identity = 39/123 (31.71%), Postives = 58/123 (47.15%), Query Frame = 0

Query: 148 LVMEKRLKKGDVEERRNRLLIP-QRKTRTTFLE------VEEEEKLNTDVWWMVEIIEPD 207
           L+ EK L   D+   +NRL +P     +  FL       ++E+   N  +     +++  
Sbjct: 228 LIFEKTLFVTDINPTQNRLSMPFNNLLQNDFLTSVESRIIKEDINNNKKIGVGAILVDQR 287

Query: 208 CTVSLITLSKWETRKGVA-----YVLITEWNGLVERNDLKEGDLMQLWSFRAGGGRGRLC 259
                + L +WE +K        Y LI  WN +VE N LKEGD + +WSFR    RG LC
Sbjct: 288 SVKWGVMLKRWELKKESGKGSWNYNLICGWNDVVEANGLKEGDNISVWSFRC---RGVLC 347

BLAST of Pay0016469 vs. ExPASy Swiss-Prot
Match: Q9SIC7 (B3 domain-containing protein At2g31420 OS=Arabidopsis thaliana OX=3702 GN=At2g31420 PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 6.1e-05
Identity = 47/180 (26.11%), Postives = 87/180 (48.33%), Query Frame = 0

Query: 87  KDLVFDTETESEGDADQNQETLTFMALAEIVESEREFLENIPEEYKKLMIFMGGSWDVCP 146
           K ++ D  + S+    QN+  +  +  A+  E+      +IP+   K+M    G +D  P
Sbjct: 27  KKIILDIPSPSQ----QNKSDIVSLCPADSPETCN---SSIPDWLVKVMRKENG-YD--P 86

Query: 147 SLVMEKR-LKKGDVEERRNRLLIPQRK-TRTTFLEVEEEEKLNTDV------WWMVEIIE 206
            L++++R L   D+ + +  L +   K  ++ FL  +E   L+ D          V +++
Sbjct: 87  KLIIKRRVLCTTDLRKNQGCLSMHLSKLEKSDFLTEDETRFLDEDFLKAKRDGLKVFLVD 146

Query: 207 PDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLMQLWSFRAGGGRGRLCFML 259
           P+    ++ L KW       YVL   WN ++++   K  D++++WSFR   G G+LCF L
Sbjct: 147 PESDKHVVYLKKWNGNTVWKYVLSHGWNNVIDKKIFKVNDVIEIWSFR--DGSGKLCFAL 194

BLAST of Pay0016469 vs. ExPASy TrEMBL
Match: A0A5A7SSE3 (B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold7305G00010 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.4e-145
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD 60
           MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD
Sbjct: 1   MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD 60

Query: 61  REKILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE 120
           REKILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE
Sbjct: 61  REKILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE 120

Query: 121 REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV 180
           REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV
Sbjct: 121 REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV 180

Query: 181 EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM 240
           EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM
Sbjct: 181 EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM 240

Query: 241 QLWSFRAGGGRGRLCFMLLEVEENRERR 269
           QLWSFRAGGGRGRLCFMLLEVEENRERR
Sbjct: 241 QLWSFRAGGGRGRLCFMLLEVEENRERR 268

BLAST of Pay0016469 vs. ExPASy TrEMBL
Match: A0A1S3B4L2 (B3 domain-containing protein At2g31420-like OS=Cucumis melo OX=3656 GN=LOC103485973 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.4e-145
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD 60
           MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD
Sbjct: 1   MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD 60

Query: 61  REKILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE 120
           REKILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE
Sbjct: 61  REKILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE 120

Query: 121 REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV 180
           REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV
Sbjct: 121 REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV 180

Query: 181 EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM 240
           EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM
Sbjct: 181 EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM 240

Query: 241 QLWSFRAGGGRGRLCFMLLEVEENRERR 269
           QLWSFRAGGGRGRLCFMLLEVEENRERR
Sbjct: 241 QLWSFRAGGGRGRLCFMLLEVEENRERR 268

BLAST of Pay0016469 vs. ExPASy TrEMBL
Match: A0A5D3C998 (B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00460 PE=4 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 2.4e-145
Identity = 267/268 (99.63%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD 60
           MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD
Sbjct: 1   MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD 60

Query: 61  REKILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE 120
           REK+LKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE
Sbjct: 61  REKLLKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE 120

Query: 121 REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV 180
           REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV
Sbjct: 121 REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV 180

Query: 181 EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM 240
           EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM
Sbjct: 181 EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM 240

Query: 241 QLWSFRAGGGRGRLCFMLLEVEENRERR 269
           QLWSFRAGGGRGRLCFMLLEVEENRERR
Sbjct: 241 QLWSFRAGGGRGRLCFMLLEVEENRERR 268

BLAST of Pay0016469 vs. ExPASy TrEMBL
Match: A0A0A0L012 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G192190 PE=4 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 9.7e-115
Identity = 219/264 (82.95%), Postives = 235/264 (89.02%), Query Frame = 0

Query: 3   MDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRDRE 62
           MDS  S+ +A+  +EQETT+STN EI+DQNPSD DDHLPLIKRKRTRMKQLARRHK  RE
Sbjct: 1   MDSRPSTSTADPPKEQETTLSTNEEISDQNPSDQDDHLPLIKRKRTRMKQLARRHKLFRE 60

Query: 63  KILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESERE 122
           K LKR L HPV+DPIPKS EEP+GKD VFD+ETESE D D NQETL F  LAE+VESE++
Sbjct: 61  KRLKRQLIHPVDDPIPKSKEEPRGKDFVFDSETESESDTDPNQETLIFRVLAEMVESEKD 120

Query: 123 FLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEVEE 182
            LE IP+EYK LMIFMGGSW+ CPSLVMEKRLKKGDVEERRNRLLIPQRKTRT FL+VEE
Sbjct: 121 SLEIIPDEYKNLMIFMGGSWNFCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTNFLDVEE 180

Query: 183 EEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLMQL 242
           EE+LN DVWWMVEIIEPDCTVSLITLSKWET KGVAYVLITEWNGLVERNDLKEGDLMQL
Sbjct: 181 EEQLNRDVWWMVEIIEPDCTVSLITLSKWETWKGVAYVLITEWNGLVERNDLKEGDLMQL 240

Query: 243 WSFRAGGGRGRLCFMLLEVEENRE 267
           WSFRAGG RGRLCFMLLEVEE RE
Sbjct: 241 WSFRAGGDRGRLCFMLLEVEEIRE 264

BLAST of Pay0016469 vs. ExPASy TrEMBL
Match: A0A5A7UZP8 (B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold39G00360 PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.8e-84
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0

Query: 111 MALAEIVESEREFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQ 170
           MALAEIVESEREFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQ
Sbjct: 1   MALAEIVESEREFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQ 60

Query: 171 RKTRTTFLEVEEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVE 230
           RKTRTTFLEVEEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVE
Sbjct: 61  RKTRTTFLEVEEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVE 120

Query: 231 RNDLKEGDLMQLWSFRAGGGRGRLCFMLLEVEENRERR 269
           RNDLKEGDLMQLWSFRAGGGRGRLCFMLLEVEENRERR
Sbjct: 121 RNDLKEGDLMQLWSFRAGGGRGRLCFMLLEVEENRERR 158

BLAST of Pay0016469 vs. NCBI nr
Match: XP_008441972.1 (PREDICTED: B3 domain-containing protein At2g31420-like [Cucumis melo] >KAA0032105.1 B3 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 525.4 bits (1352), Expect = 2.9e-145
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD 60
           MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD
Sbjct: 1   MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD 60

Query: 61  REKILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE 120
           REKILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE
Sbjct: 61  REKILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE 120

Query: 121 REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV 180
           REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV
Sbjct: 121 REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV 180

Query: 181 EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM 240
           EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM
Sbjct: 181 EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM 240

Query: 241 QLWSFRAGGGRGRLCFMLLEVEENRERR 269
           QLWSFRAGGGRGRLCFMLLEVEENRERR
Sbjct: 241 QLWSFRAGGGRGRLCFMLLEVEENRERR 268

BLAST of Pay0016469 vs. NCBI nr
Match: TYK08431.1 (B3 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 524.6 bits (1350), Expect = 4.9e-145
Identity = 267/268 (99.63%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD 60
           MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD
Sbjct: 1   MEMDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRD 60

Query: 61  REKILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE 120
           REK+LKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE
Sbjct: 61  REKLLKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESE 120

Query: 121 REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV 180
           REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV
Sbjct: 121 REFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEV 180

Query: 181 EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM 240
           EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM
Sbjct: 181 EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLM 240

Query: 241 QLWSFRAGGGRGRLCFMLLEVEENRERR 269
           QLWSFRAGGGRGRLCFMLLEVEENRERR
Sbjct: 241 QLWSFRAGGGRGRLCFMLLEVEENRERR 268

BLAST of Pay0016469 vs. NCBI nr
Match: XP_004147556.1 (B3 domain-containing protein At2g31420 [Cucumis sativus] >KGN53947.1 hypothetical protein Csa_021583 [Cucumis sativus])

HSP 1 Score: 422.9 bits (1086), Expect = 2.0e-114
Identity = 219/264 (82.95%), Postives = 235/264 (89.02%), Query Frame = 0

Query: 3   MDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRDRE 62
           MDS  S+ +A+  +EQETT+STN EI+DQNPSD DDHLPLIKRKRTRMKQLARRHK  RE
Sbjct: 1   MDSRPSTSTADPPKEQETTLSTNEEISDQNPSDQDDHLPLIKRKRTRMKQLARRHKLFRE 60

Query: 63  KILKRPLKHPVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQETLTFMALAEIVESERE 122
           K LKR L HPV+DPIPKS EEP+GKD VFD+ETESE D D NQETL F  LAE+VESE++
Sbjct: 61  KRLKRQLIHPVDDPIPKSKEEPRGKDFVFDSETESESDTDPNQETLIFRVLAEMVESEKD 120

Query: 123 FLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEVEE 182
            LE IP+EYK LMIFMGGSW+ CPSLVMEKRLKKGDVEERRNRLLIPQRKTRT FL+VEE
Sbjct: 121 SLEIIPDEYKNLMIFMGGSWNFCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTNFLDVEE 180

Query: 183 EEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLMQL 242
           EE+LN DVWWMVEIIEPDCTVSLITLSKWET KGVAYVLITEWNGLVERNDLKEGDLMQL
Sbjct: 181 EEQLNRDVWWMVEIIEPDCTVSLITLSKWETWKGVAYVLITEWNGLVERNDLKEGDLMQL 240

Query: 243 WSFRAGGGRGRLCFMLLEVEENRE 267
           WSFRAGG RGRLCFMLLEVEE RE
Sbjct: 241 WSFRAGGDRGRLCFMLLEVEEIRE 264

BLAST of Pay0016469 vs. NCBI nr
Match: XP_038883177.1 (B3 domain-containing protein At1g05920-like [Benincasa hispida])

HSP 1 Score: 341.7 bits (875), Expect = 5.9e-90
Identity = 178/271 (65.68%), Postives = 217/271 (80.07%), Query Frame = 0

Query: 3   MDSNTSSPSANAQQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQLARRHKRDRE 62
           M  + ++P+ +  ++QETTVS     TD+N SD+DDHLPLIKRKRT++   ++RHK  RE
Sbjct: 1   MHFDRTNPTGDVDKDQETTVSAKEGTTDRNLSDEDDHLPLIKRKRTKVHISSKRHKLLRE 60

Query: 63  KILKRPLKHPVNDPIPKSDEEPQ---GKDLVFDTETESEGDADQNQETLTFMALAEIVES 122
           + LKRPL   + DPIPK DE+P+    K+L+F  E+ES+ D D+N+E L    LA+I E+
Sbjct: 61  RRLKRPLPLRIADPIPKFDEQPEESPRKNLIFVAESESDSDTDRNREALAIAGLADIAET 120

Query: 123 EREFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLE 182
            +E ++NIPEEYK LMIFMGGS    P+LV++KRL+KGDVEERRNRLLIPQRK +T FLE
Sbjct: 121 NQELIQNIPEEYKNLMIFMGGSRSASPNLVLQKRLRKGDVEERRNRLLIPQRKIKTNFLE 180

Query: 183 VEEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDL 242
           VEEEEKLN D+WWMVEIIEPDC+VSLITLSKWET KGVAYV ITEWNGLVERN+LKEGDL
Sbjct: 181 VEEEEKLNRDIWWMVEIIEPDCSVSLITLSKWETWKGVAYVFITEWNGLVERNELKEGDL 240

Query: 243 MQLWSFRAGGG----RGRLCFMLLEVEENRE 267
           MQLWSFRA GG    RGRLCFMLLE+++NRE
Sbjct: 241 MQLWSFRAAGGGGGRRGRLCFMLLELDDNRE 271

BLAST of Pay0016469 vs. NCBI nr
Match: KAA0060117.1 (B3 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 322.4 bits (825), Expect = 3.7e-84
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0

Query: 111 MALAEIVESEREFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQ 170
           MALAEIVESEREFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQ
Sbjct: 1   MALAEIVESEREFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQ 60

Query: 171 RKTRTTFLEVEEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVE 230
           RKTRTTFLEVEEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVE
Sbjct: 61  RKTRTTFLEVEEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVE 120

Query: 231 RNDLKEGDLMQLWSFRAGGGRGRLCFMLLEVEENRERR 269
           RNDLKEGDLMQLWSFRAGGGRGRLCFMLLEVEENRERR
Sbjct: 121 RNDLKEGDLMQLWSFRAGGGRGRLCFMLLEVEENRERR 158

BLAST of Pay0016469 vs. TAIR 10
Match: AT5G24050.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 59.3 bits (142), Expect = 5.4e-09
Identity = 48/143 (33.57%), Postives = 66/143 (46.15%), Query Frame = 0

Query: 128 PEEYKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIP-QRKTRTTFLE------V 187
           PE   K+M  M  + D    L+ EK L   DV   +NRL +P     R  FL       +
Sbjct: 200 PEWLVKVMTNMKEAKDA--KLIFEKTLFVTDVNPTKNRLSMPFNNLLRNDFLTSVESIII 259

Query: 188 EEEEKLNTDVWWMVEIIEPDCTVSLITLSKWETRK-----GVAYVLITEWNGLVERNDLK 247
            E+   N  +     +++  C    + L +WE +K       +Y LI  WN +VE N LK
Sbjct: 260 NEDINNNNKIGVGAILVDQRCKKWGVMLKRWEMKKESGKGSWSYNLICGWNDIVEANRLK 319

Query: 248 EGDLMQLWSFRAGGGRGRLCFML 259
           EGD + LWSFR     G LCF +
Sbjct: 320 EGDNITLWSFRC---CGILCFAM 337

BLAST of Pay0016469 vs. TAIR 10
Match: AT1G05920.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 53.5 bits (127), Expect = 3.0e-07
Identity = 69/242 (28.51%), Postives = 109/242 (45.04%), Query Frame = 0

Query: 47  RTRMKQLARRHKRDREKIL-------KRPLKHPVNDPIPK----SDEEPQGKDLVFDTET 106
           RT+ K +A+     R++         + P  +  NDP P+        P+ KD++ D   
Sbjct: 82  RTKPKTVAQVRDAKRKRTFDHVPRGTREPHAYLRNDPAPQVASVPKSVPEEKDVILD--- 141

Query: 107 ESEGDADQNQETLTFMALAEIVESEREFLENIPEEYKKLMIFMGGSWDVCPSLVMEKRLK 166
               +  + ++T ++    E V  +       P+   ++M  M G+ D  P L++EK L 
Sbjct: 142 RILSNVPRRKKTTSY----EFVPPKH---PQEPQWLLQVMSRMNGAGD--PKLIIEKNLD 201

Query: 167 KGDVEERRNRLLIP-QRKTRTTFLEVEEEEKLNTDVWWMVEII-EPDCTVSLITL----S 226
             DV+ R+NRL IP     +  FL ++E   ++ D     EI  E +  V+   +     
Sbjct: 202 SNDVDPRQNRLSIPINTVIQNDFLTLDESRLIDED-----EITNEGNMGVAAFLVDQRTK 261

Query: 227 KWE---------TRKGVAY---VLITEWNGLVERNDLKEGDLMQLWSFRAGGGRGRLCFM 260
           KW          T  G +Y   VL  EW+ +VE N LKEGD + LWSFR+      LCF 
Sbjct: 262 KWNMGFKQWFMTTDSGSSYWSFVLRGEWSNVVETNGLKEGDKISLWSFRS---NDILCFA 303

BLAST of Pay0016469 vs. TAIR 10
Match: AT3G24850.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 52.4 bits (124), Expect = 6.7e-07
Identity = 39/123 (31.71%), Postives = 58/123 (47.15%), Query Frame = 0

Query: 148 LVMEKRLKKGDVEERRNRLLIP-QRKTRTTFLE------VEEEEKLNTDVWWMVEIIEPD 207
           L+ EK L   D+   +NRL +P     +  FL       ++E+   N  +     +++  
Sbjct: 228 LIFEKTLFVTDINPTQNRLSMPFNNLLQNDFLTSVESRIIKEDINNNKKIGVGAILVDQR 287

Query: 208 CTVSLITLSKWETRKGVA-----YVLITEWNGLVERNDLKEGDLMQLWSFRAGGGRGRLC 259
                + L +WE +K        Y LI  WN +VE N LKEGD + +WSFR    RG LC
Sbjct: 288 SVKWGVMLKRWELKKESGKGSWNYNLICGWNDVVEANGLKEGDNISVWSFRC---RGVLC 347

BLAST of Pay0016469 vs. TAIR 10
Match: AT2G31420.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 49.7 bits (117), Expect = 4.3e-06
Identity = 47/180 (26.11%), Postives = 87/180 (48.33%), Query Frame = 0

Query: 87  KDLVFDTETESEGDADQNQETLTFMALAEIVESEREFLENIPEEYKKLMIFMGGSWDVCP 146
           K ++ D  + S+    QN+  +  +  A+  E+      +IP+   K+M    G +D  P
Sbjct: 27  KKIILDIPSPSQ----QNKSDIVSLCPADSPETCN---SSIPDWLVKVMRKENG-YD--P 86

Query: 147 SLVMEKR-LKKGDVEERRNRLLIPQRK-TRTTFLEVEEEEKLNTDV------WWMVEIIE 206
            L++++R L   D+ + +  L +   K  ++ FL  +E   L+ D          V +++
Sbjct: 87  KLIIKRRVLCTTDLRKNQGCLSMHLSKLEKSDFLTEDETRFLDEDFLKAKRDGLKVFLVD 146

Query: 207 PDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLMQLWSFRAGGGRGRLCFML 259
           P+    ++ L KW       YVL   WN ++++   K  D++++WSFR   G G+LCF L
Sbjct: 147 PESDKHVVYLKKWNGNTVWKYVLSHGWNNVIDKKIFKVNDVIEIWSFR--DGSGKLCFAL 194

BLAST of Pay0016469 vs. TAIR 10
Match: AT5G38500.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 49.3 bits (116), Expect = 5.6e-06
Identity = 43/148 (29.05%), Postives = 61/148 (41.22%), Query Frame = 0

Query: 146 PSLVMEKRLKKGDVEERRNRLLIP-QRKTRTTFLEVEE---------------------- 205
           P L+ E+ L K DV    +RLLIP Q+  R  FL  EE                      
Sbjct: 264 PKLIFERTLFKSDVNSNLSRLLIPFQKLIRNDFLTPEECRAMQEDKDKDDEDISVGTILV 323

Query: 206 -------EEKLNTDVWWMVEIIEPDCTVSLITLSKWETRKG-----VAYVLITEWNGLVE 259
                  E+K + D+     ++     +  +    W   K      + Y+L  +WN +V+
Sbjct: 324 CQAKQEDEDKDDEDIGAGTILVNQRFKMWGLRFKIWGMEKDSGHGTLNYILNWDWNDVVK 383

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLV77.7e-0833.57B3 domain-containing protein At5g24050 OS=Arabidopsis thaliana OX=3702 GN=At5g24... [more]
Q9LSF76.5e-0734.96B3 domain-containing protein At3g25182 OS=Arabidopsis thaliana OX=3702 GN=At3g25... [more]
Q5RM094.2e-0628.51B3 domain-containing protein At1g05920 OS=Arabidopsis thaliana OX=3702 GN=At1g05... [more]
Q9LRX69.4e-0631.71Putative B3 domain-containing protein At3g24850 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9SIC76.1e-0526.11B3 domain-containing protein At2g31420 OS=Arabidopsis thaliana OX=3702 GN=At2g31... [more]
Match NameE-valueIdentityDescription
A0A5A7SSE31.4e-145100.00B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3B4L21.4e-145100.00B3 domain-containing protein At2g31420-like OS=Cucumis melo OX=3656 GN=LOC103485... [more]
A0A5D3C9982.4e-14599.63B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A0A0L0129.7e-11582.95Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G192190 PE=4 SV=1[more]
A0A5A7UZP81.8e-84100.00B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
Match NameE-valueIdentityDescription
XP_008441972.12.9e-145100.00PREDICTED: B3 domain-containing protein At2g31420-like [Cucumis melo] >KAA003210... [more]
TYK08431.14.9e-14599.63B3 domain-containing protein [Cucumis melo var. makuwa][more]
XP_004147556.12.0e-11482.95B3 domain-containing protein At2g31420 [Cucumis sativus] >KGN53947.1 hypothetica... [more]
XP_038883177.15.9e-9065.68B3 domain-containing protein At1g05920-like [Benincasa hispida][more]
KAA0060117.13.7e-84100.00B3 domain-containing protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT5G24050.15.4e-0933.57Domain of unknown function (DUF313) [more]
AT1G05920.13.0e-0728.51Domain of unknown function (DUF313) [more]
AT3G24850.16.7e-0731.71Domain of unknown function (DUF313) [more]
AT2G31420.14.3e-0626.11Domain of unknown function (DUF313) [more]
AT5G38500.15.6e-0629.05Domain of unknown function (DUF313) [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 43..63
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 66..87
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..87
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..65
NoneNo IPR availablePANTHERPTHR31541:SF25DOMAIN PROTEIN, PUTATIVE (DUF313)-RELATEDcoord: 14..265
IPR015300DNA-binding pseudobarrel domain superfamilyGENE3D2.40.330.10coord: 146..258
e-value: 1.1E-13
score: 52.9
IPR015300DNA-binding pseudobarrel domain superfamilySUPERFAMILY101936DNA-binding pseudobarrel domaincoord: 150..248
IPR005508B3 domain-containing protein At2g31720-likePFAMPF03754DUF313coord: 144..230
e-value: 2.1E-8
score: 34.3
IPR005508B3 domain-containing protein At2g31720-likePANTHERPTHR31541B3 DOMAIN PLANT PROTEIN-RELATEDcoord: 14..265

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0016469.1Pay0016469.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding