Pay0016288 (gene) Melon (Payzawat) v1

Overview
NamePay0016288
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Descriptionmitogen-activated protein kinase kinase kinase 2-like
Locationchr11: 10970739 .. 10971957 (-)
RNA-Seq ExpressionPay0016288
SyntenyPay0016288
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTGGACTAGAGGCCACGTCATTGGCCACGGCTCCTCCGCCACCGTTTTTCTCGCTACCGATTCTCCCTCCGGCGATGTCTTCGCCGTCAAAACTGCCCAGCTTTCGCAGTCGCAGTCTCTTCGCAAGGAGCAACAGTTTCTTTCTTCTTTAGCCTCTCCTTACATAGTTTCTTATAGAGGCTTTGAAGTCCGTAGAGAACAGAGTGGGGTCATGATGTTCAATCTTTTCATGGAGTACTTACCCAACGGCTCACTCGCCGACACAATTCGCCGCCGTGGAGGACAACGGCTCGACGAGGCAACGATTGCAATCTACACAAGGCAGTTACTAATGGGTCTACAATACATTCATTCCAAAGGCATAGTACATTGCGACATTAAAGCCCGAAACATTTTGATTGGTCTAGACGGCGAGGCTAAATTGGCTGATTTCGGTTGCGCCAAGTGGGCAACAAGCCAAACGGATCCAATCGGCGGCACGCCCTTGTTCATGGCGCCGGAAGTAGCTCGTGGTGAACACCAAGGTTTCCCCTCCGACATTTGGTCAATTGGGTGTACAATTATCGAAATGGGCACCGGTGGCGGCTCGCCTTGGCCAAAAACAACCGACGACACCGACCCGATTTCTGCTCTGTATCGGATTGGGTATTCCGGGGAGTCGCCGGAAATTCCATGTTATTTATCTGAAGAAGCAAAGGATTTTGTAGAAGGCCATGTAAAACGGAGGAAGTTCATTCAGAATCGCCGACGAGTATTCTAGAACAGGGGATATGGAGATCGATTGAAGAAAGTGAAATTAGGGGAAGGGAATTGGTAAGATCCGATGGTTGGGAGGCAGCGGAAGCAGAGGAGCAGATCCGACGGCTGTGGAGATTTCAGGGGAGCCCAGATGGGAAGAGGATGAGAATTGGATTACAATTAGAAGGAAAGAAGAAGGAGAGAAGAATGGTGGGGCAGATGAGCCAGAAGTGAAAAAATGTAGTAATTATTCTAAAAATAATAATAATAATAGTAATTGGGGTGAGAAAAAGAGAGGTAGTGGAGGGGAAGAGTGGTTGGAAATGGAATTAGGGAATATTAGTTGTGGAATTAGTAGGAATGATTTGGGAGACTATAGTTGTAGGAATATTATTAGTCTTGTAAATAATAATAATCCTTTATCTTTTCATACACTTACACCTATATTTCTTCCTTTAATGCCAACTATATTATGA

mRNA sequence

ATGGACTGGACTAGAGGCCACGTCATTGGCCACGGCTCCTCCGCCACCGTTTTTCTCGCTACCGATTCTCCCTCCGGCGATGTCTTCGCCGTCAAAACTGCCCAGCTTTCGCAGTCGCAGTCTCTTCGCAAGGAGCAACAGTTTCTTTCTTCTTTAGCCTCTCCTTACATAGTTTCTTATAGAGGCTTTGAAGTCCGTAGAGAACAGAGTGGGGTCATGATGTTCAATCTTTTCATGGAGTACTTACCCAACGGCTCACTCGCCGACACAATTCGCCGCCGTGGAGGACAACGGCTCGACGAGGCAACGATTGCAATCTACACAAGGCAGTTACTAATGGGTCTACAATACATTCATTCCAAAGGCATAGTACATTGCGACATTAAAGCCCGAAACATTTTGATTGGTCTAGACGGCGAGGCTAAATTGGCTGATTTCGGTTGCGCCAAGTGGGCAACAAGCCAAACGGATCCAATCGGCGGCACGCCCTTGTTCATGGCGCCGGAAGTAGCTCGTGGTGAACACCAAGGTTTCCCCTCCGACATTTGGTCAATTGGGTGTACAATTATCGAAATGGGCACCGGTGGCGGCTCGCCTTGGCCAAAAACAACCGACGACACCGACCCGATTTCTGCTCTGTATCGGATTGGGTATTCCGGGGAAAGCAAAGGATTTTGTAGAAGGCCATGTAAAACGGAGGAAGTTCATTCAGAATCGCCGACGAGTATTCTAGAACAGGGGATATGGAGATCGATTGAAGAAAGTGAAATTAGGGGAAGGGAATTGGTAAGATCCGATGGTTGGGAGGCAGCGGAAGCAGAGGAGCAGATCCGACGGCTAAGGAAAGAAGAAGGAGAGAAGAATGGTGGGGCAGATGAGCCAGAAGTGAAAAAATGTAGTAATTATTCTAAAAATAATAATAATAATAGTAATTGGGGTGAGAAAAAGAGAGGTAGTGGAGGGGAAGAGTGGTTGGAAATGGAATTAGGGAATATTAGTTGTGGAATTAGTAGGAATGATTTGGGAGACTATAGTTGTAGGAATATTATTAGTCTTGTAAATAATAATAATCCTTTATCTTTTCATACACTTACACCTATATTTCTTCCTTTAATGCCAACTATATTATGA

Coding sequence (CDS)

ATGGACTGGACTAGAGGCCACGTCATTGGCCACGGCTCCTCCGCCACCGTTTTTCTCGCTACCGATTCTCCCTCCGGCGATGTCTTCGCCGTCAAAACTGCCCAGCTTTCGCAGTCGCAGTCTCTTCGCAAGGAGCAACAGTTTCTTTCTTCTTTAGCCTCTCCTTACATAGTTTCTTATAGAGGCTTTGAAGTCCGTAGAGAACAGAGTGGGGTCATGATGTTCAATCTTTTCATGGAGTACTTACCCAACGGCTCACTCGCCGACACAATTCGCCGCCGTGGAGGACAACGGCTCGACGAGGCAACGATTGCAATCTACACAAGGCAGTTACTAATGGGTCTACAATACATTCATTCCAAAGGCATAGTACATTGCGACATTAAAGCCCGAAACATTTTGATTGGTCTAGACGGCGAGGCTAAATTGGCTGATTTCGGTTGCGCCAAGTGGGCAACAAGCCAAACGGATCCAATCGGCGGCACGCCCTTGTTCATGGCGCCGGAAGTAGCTCGTGGTGAACACCAAGGTTTCCCCTCCGACATTTGGTCAATTGGGTGTACAATTATCGAAATGGGCACCGGTGGCGGCTCGCCTTGGCCAAAAACAACCGACGACACCGACCCGATTTCTGCTCTGTATCGGATTGGGTATTCCGGGGAAAGCAAAGGATTTTGTAGAAGGCCATGTAAAACGGAGGAAGTTCATTCAGAATCGCCGACGAGTATTCTAGAACAGGGGATATGGAGATCGATTGAAGAAAGTGAAATTAGGGGAAGGGAATTGGTAAGATCCGATGGTTGGGAGGCAGCGGAAGCAGAGGAGCAGATCCGACGGCTAAGGAAAGAAGAAGGAGAGAAGAATGGTGGGGCAGATGAGCCAGAAGTGAAAAAATGTAGTAATTATTCTAAAAATAATAATAATAATAGTAATTGGGGTGAGAAAAAGAGAGGTAGTGGAGGGGAAGAGTGGTTGGAAATGGAATTAGGGAATATTAGTTGTGGAATTAGTAGGAATGATTTGGGAGACTATAGTTGTAGGAATATTATTAGTCTTGTAAATAATAATAATCCTTTATCTTTTCATACACTTACACCTATATTTCTTCCTTTAATGCCAACTATATTATGA

Protein sequence

MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSYRGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHSKGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPSDIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGESKGFCRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRELVRSDGWEAAEAEEQIRRLRKEEGEKNGGADEPEVKKCSNYSKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSFHTLTPIFLPLMPTIL
Homology
BLAST of Pay0016288 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.5e-70
Identity = 139/306 (45.42%), Postives = 187/306 (61.11%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           M+WTRG ++G GS+ATV+ A    S ++ AVK++++ +S+ L++E + LSSL+SPY++ Y
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120
           RG E +RE +GV+M+NL MEY P G+L D   + GG R+DE  +  YTR +L GL+YIHS
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGG-RVDETRVVKYTRDILKGLEYIHS 120

Query: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTD-PIGGTPLFMAPEVARGEHQGFP 180
           KGIVHCD+K  N++I   GEAK+ADFGCAK      + P+ GTP FMAPEVARGE QG  
Sbjct: 121 KGIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKE 180

Query: 181 SDIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSG-----------ESKGFCRR 240
           SDIW++GCT+IEM T G  PW K     DP+S LYR+GYS            E+K F  +
Sbjct: 181 SDIWAVGCTMIEMVT-GSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEK 240

Query: 241 PCKTEE----------------------------VHSESPTSILEQGIWRSIEESEIRGR 267
             K E                             + S SPTS+ +Q  WRS+EE E    
Sbjct: 241 CLKREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEEEEET 300

BLAST of Pay0016288 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 7.8e-67
Identity = 144/316 (45.57%), Postives = 185/316 (58.54%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           M+WTRG  +G GS+ATV  AT   SG+  AVK+A+  +S+ L++E + LSSL SPY++ Y
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  RGFEVRRE----QSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQ 120
           RG E+ RE          ++L MEY P G+L D   + GG  +DEA +  YTRQ+L+GL+
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGG-FIDEARVVKYTRQILLGLE 120

Query: 121 YIH-SKGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQ-TDPIGGTPLFMAPEVARGE 180
           YIH SKGI HCDIK  N+L+G +GEAK+ADFGCAKW   + T+P+ GTP FMAPE ARGE
Sbjct: 121 YIHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPVRGTPAFMAPEAARGE 180

Query: 181 HQGFPSDIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGE-----------SK 240
            QG  SDIW++GCT+IEM T G  PW    D TDP+S LYR+GY GE           +K
Sbjct: 181 RQGKESDIWAVGCTVIEMVT-GSQPW-IGADFTDPVSVLYRVGYLGELPELPCSLTEQAK 240

Query: 241 GFCRRPCKTEEVH-------------------------SESPTSILEQGIWRSIEESEIR 275
            F  +  K E                            + SPTS+ +Q  WRS+EE    
Sbjct: 241 DFLGKCLKKEATERWTASQLLNHPFLVNKEPELVTGLVTNSPTSVTDQMFWRSVEEEVSE 300

BLAST of Pay0016288 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.1e-35
Identity = 92/232 (39.66%), Postives = 129/232 (55.60%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDS-PSGD---VFAVKTAQLSQSQSLRKEQQFLSSLAS-P 60
           M+W RG  IG G+ +TV  AT S  SGD   + AVK+     + SL  E+  L SL   P
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  YIVSYRGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGL 120
            I+   G E    ++G  M NL +EY   GSLA  +++ GG+ L E+T+  +T  +L GL
Sbjct: 61  EIIRCYG-EDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 120

Query: 121 QYIHSKGIVHCDIKARNILIGLDGEAKLADFGCAK------WATSQTDPIGGTPLFMAPE 180
           ++IH+KG  HCDIK  NIL+  DG  K+ADFG A        A  ++  I GTPL+MAPE
Sbjct: 121 RHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPE 180

Query: 181 VARGEHQGFPSDIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGE 222
                  G  +D+W++GC ++EM   G + W    + +  +S L RIG   E
Sbjct: 181 CVNDNEYGSAADVWALGCAVVEM-FSGKTAW-SVKEGSHFMSLLIRIGVGDE 229

BLAST of Pay0016288 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.7e-35
Identity = 98/283 (34.63%), Postives = 144/283 (50.88%), Query Frame = 0

Query: 3   WTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQS-----------QSLRKEQQFLSS 62
           W +G +IG G+   V++  +  SG++ A+K   ++ +           + L +E   L +
Sbjct: 80  WRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASRERAQAHVRELEEEVNLLKN 139

Query: 63  LASPYIVSYRGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQL 122
           L+ P IV Y G    RE   +   N+ +E++P GS++  + + G     E+ I +YT+QL
Sbjct: 140 LSHPNIVRYLG--TAREAGSL---NILLEFVPGGSISSLLGKFGS--FPESVIRMYTKQL 199

Query: 123 LMGLQYIHSKGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIG-----GTPLFM 182
           L+GL+Y+H  GI+H DIK  NIL+   G  KLADFG +K         G     GTP +M
Sbjct: 200 LLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWM 259

Query: 183 APEVARGEHQGFPSDIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIG--------- 242
           APEV       F +DIWS+GCTIIEM T G  PW +   +   ++AL+ IG         
Sbjct: 260 APEVILQTGHSFSADIWSVGCTIIEMAT-GKPPWSQQYQE---VAALFHIGTTKSHPPIP 319

Query: 243 --YSGESKGFCRRPCKTEEVHSESPTSILEQGIWRSIEESEIR 259
              S ESK F  + C  +E H     S L Q  + + E  E R
Sbjct: 320 EHLSAESKDFLLK-CLQKEPHLRHSASNLLQHPFVTAEHQEAR 350

BLAST of Pay0016288 vs. ExPASy Swiss-Prot
Match: Q9FZ36 (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ANP2 PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.0e-34
Identity = 84/231 (36.36%), Postives = 130/231 (56.28%), Query Frame = 0

Query: 3   WTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQS-----------QSLRKEQQFLSS 62
           W +G +IG G+  TV++  +  SG++ AVK   ++ +           Q L +E + L +
Sbjct: 68  WRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEVKLLKN 127

Query: 63  LASPYIVSYRGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQL 122
           L+ P IV Y G  VR +++     N+ +E++P GS++  + + G     E+ +  YT QL
Sbjct: 128 LSHPNIVRYLG-TVREDET----LNILLEFVPGGSISSLLEKFGA--FPESVVRTYTNQL 187

Query: 123 LMGLQYIHSKGIVHCDIKARNILIGLDGEAKLADFGCAKWAT-----SQTDPIGGTPLFM 182
           L+GL+Y+H+  I+H DIK  NIL+   G  KLADFG +K        S    + GTP +M
Sbjct: 188 LLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWM 247

Query: 183 APEVARGEHQGFPSDIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIG 218
           APEV       F +DIWS+GCT+IEM T G +PW +   +   I+A++ IG
Sbjct: 248 APEVILQTGHSFSADIWSVGCTVIEMVT-GKAPWSQQYKE---IAAIFHIG 287

BLAST of Pay0016288 vs. ExPASy TrEMBL
Match: A0A5D3CC81 (Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold458G00410 PE=3 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 1.9e-204
Identity = 373/434 (85.94%), Postives = 374/434 (86.18%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQ FLSSLASPYIVSY
Sbjct: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQHFLSSLASPYIVSY 60

Query: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120
           RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS
Sbjct: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120

Query: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180
           KG+VHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS
Sbjct: 121 KGVVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180

Query: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES-----------KGF---- 240
           DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES           K F    
Sbjct: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGESPEIPCYLSEEAKDFLEKC 240

Query: 241 ------------------------CRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300
                                   CRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE
Sbjct: 241 LKRNPSERWTANELMNHPFLKELNCRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300

Query: 301 LVRSDGWEAAEAEEQIRRL-------------------RKEEGEKNGGADEPEVKKCSNY 360
           LVRSDGWEAAEAEEQIRRL                   RKEEGEKNGGADEPEVKKCSNY
Sbjct: 301 LVRSDGWEAAEAEEQIRRLWMISGEPRWEEDENWITIRRKEEGEKNGGADEPEVKKCSNY 360

Query: 361 SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSFH 377
           SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSFH
Sbjct: 361 SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSFH 420

BLAST of Pay0016288 vs. ExPASy TrEMBL
Match: A0A1S3C2N9 (mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103495976 PE=3 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 1.9e-204
Identity = 373/434 (85.94%), Postives = 374/434 (86.18%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQ FLSSLASPYIVSY
Sbjct: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQHFLSSLASPYIVSY 60

Query: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120
           RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS
Sbjct: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120

Query: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180
           KG+VHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS
Sbjct: 121 KGVVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180

Query: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES-----------KGF---- 240
           DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES           K F    
Sbjct: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGESPEIPCYLSEEAKDFLEKC 240

Query: 241 ------------------------CRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300
                                   CRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE
Sbjct: 241 LKRNPSERWTANELMNHPFLKELNCRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300

Query: 301 LVRSDGWEAAEAEEQIRRL-------------------RKEEGEKNGGADEPEVKKCSNY 360
           LVRSDGWEAAEAEEQIRRL                   RKEEGEKNGGADEPEVKKCSNY
Sbjct: 301 LVRSDGWEAAEAEEQIRRLWMISGEPRWEEDENWITIRRKEEGEKNGGADEPEVKKCSNY 360

Query: 361 SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSFH 377
           SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSFH
Sbjct: 361 SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSFH 420

BLAST of Pay0016288 vs. ExPASy TrEMBL
Match: A0A5A7SY50 (Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold213G00370 PE=3 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 5.4e-204
Identity = 373/434 (85.94%), Postives = 374/434 (86.18%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY
Sbjct: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60

Query: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120
           RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS
Sbjct: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120

Query: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180
           KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS
Sbjct: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180

Query: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES-----------KGF---- 240
           DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES           K F    
Sbjct: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGESPEIPCYLSEEAKDFLEKC 240

Query: 241 ------------------------CRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300
                                   CRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE
Sbjct: 241 LKRNPSERWTANELMNHPFLKELNCRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300

Query: 301 LVRSDGWEAAEAEEQIRRL-------------------RKEEGEKNGGADEPEVKKCSNY 360
           LVRSD WEAAEAEEQIRRL                   RKEEGEKNGGADEPEVKKCSNY
Sbjct: 301 LVRSDCWEAAEAEEQIRRLWTISGEPRWEEDENWITIRRKEEGEKNGGADEPEVKKCSNY 360

Query: 361 SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSFH 377
           SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNI+SLVNNNNPLSFH
Sbjct: 361 SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIVSLVNNNNPLSFH 420

BLAST of Pay0016288 vs. ExPASy TrEMBL
Match: A0A0A0LN49 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G416770 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 2.1e-187
Identity = 352/435 (80.92%), Postives = 359/435 (82.53%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           MDWTRGHVIGHGSSATVFLATDSPS  VFAVKTAQLS SQSLRKEQQFLSSLASPYIVSY
Sbjct: 1   MDWTRGHVIGHGSSATVFLATDSPSRHVFAVKTAQLSHSQSLRKEQQFLSSLASPYIVSY 60

Query: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120
           RGFEV REQSGV MFNLFMEYLPNGSLADTIRRRGGQRLDEATI IYTRQ+LMGLQYIHS
Sbjct: 61  RGFEVSREQSGVTMFNLFMEYLPNGSLADTIRRRGGQRLDEATIVIYTRQILMGLQYIHS 120

Query: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180
           KGIVHCDIKARNILIGLDGEAKLADFGCAK ATSQTDPI GTPLFMAPEVARGEHQGFPS
Sbjct: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKRATSQTDPICGTPLFMAPEVARGEHQGFPS 180

Query: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES-----------KGF---- 240
           DIWSIGCTIIEM +GGGSPWPKTTDDTDPISALYRIGYSGES           K F    
Sbjct: 181 DIWSIGCTIIEMASGGGSPWPKTTDDTDPISALYRIGYSGESPEIPCYLSEEAKDFLEKC 240

Query: 241 ------------------------CRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300
                                   CRR  KTEEVHSESPTSILEQGIWRSIEESEIRGRE
Sbjct: 241 LKRNPSERWTASELMNHPFLRELNCRREWKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300

Query: 301 LVRSDGWEAAEAEEQIRRL-------------------RKEEGEKNGGADEPEVKKCSNY 360
           LVRS+GWEAA AEEQIRRL                   RKEEGEKNGGADE EVKKCSNY
Sbjct: 301 LVRSNGWEAAAAEEQIRRLWMISGEPRWEEDENWITIRRKEEGEKNGGADESEVKKCSNY 360

Query: 361 S-KNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSF 377
           S  NNNNNSNWG+KKRGSGG+EWL+MELGNISC ISRNDLGDYSCRN+ISLVNNN PLSF
Sbjct: 361 SNNNNNNNSNWGDKKRGSGGKEWLQMELGNISCRISRNDLGDYSCRNVISLVNNNYPLSF 420

BLAST of Pay0016288 vs. ExPASy TrEMBL
Match: A0A6J1JDQ6 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111484093 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.5e-142
Identity = 286/450 (63.56%), Postives = 311/450 (69.11%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           MDWTR HVIGHGSSATVFLATDSPSGDVFAVK+A++S SQSL+ EQQFLSSLASPY+VSY
Sbjct: 1   MDWTRRHVIGHGSSATVFLATDSPSGDVFAVKSAEISHSQSLQTEQQFLSSLASPYVVSY 60

Query: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120
           +G+EVRREQSGV+MFNLFMEYLPNGSLAD IRRRGG+RLDEA I IYTRQLL GLQYIHS
Sbjct: 61  KGYEVRREQSGVVMFNLFMEYLPNGSLADVIRRRGGRRLDEAAIGIYTRQLLQGLQYIHS 120

Query: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180
           KG+VHCDIK+RN+LIG DGEAKLADFGCAKWA SQTD IGGTPLFMAPEVARGEHQG PS
Sbjct: 121 KGLVHCDIKSRNVLIGRDGEAKLADFGCAKWA-SQTDSIGGTPLFMAPEVARGEHQGLPS 180

Query: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES------------------ 240
           DIWSIGC++IEM TGGGSPWPKTT D+DPISALYRIGYSGES                  
Sbjct: 181 DIWSIGCSVIEMATGGGSPWPKTTSDSDPISALYRIGYSGESPEIPSFLSHKAKDFLEKC 240

Query: 241 --------------------KGFCRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGREL 300
                               + F   P K  EVHS SPTSILEQG+WRSIEESEIR  + 
Sbjct: 241 LRMNPNERWTANQLLNHPFVREFNSSPKKIHEVHSNSPTSILEQGVWRSIEESEIREAKS 300

Query: 301 VRSDGWEAAEAEEQIRRLRKEEGEKNGGADE-------------------------PEVK 360
            R + W    A EQIRRL    GE +   DE                          EVK
Sbjct: 301 CRPNSW----AAEQIRRLWMASGEPSWRDDETWITIRRQDIGKSKEVGDEGDTIGGSEVK 360

Query: 361 KCSNYSKNNNNNSNWGEKKRGSGGEEWLEMEL----GNISCGISRNDLGDYSCRNIISLV 377
            CS        N+ WGEK+RG GG+EW E E     G  SC ISRNDL D+ C+NIISLV
Sbjct: 361 GCS--------NNCWGEKQRGGGGKEWSETEFTQNNGGFSCRISRNDLADHRCKNIISLV 420

BLAST of Pay0016288 vs. NCBI nr
Match: XP_008455899.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo] >TYK09607.1 mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 721.5 bits (1861), Expect = 3.8e-204
Identity = 373/434 (85.94%), Postives = 374/434 (86.18%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQ FLSSLASPYIVSY
Sbjct: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQHFLSSLASPYIVSY 60

Query: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120
           RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS
Sbjct: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120

Query: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180
           KG+VHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS
Sbjct: 121 KGVVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180

Query: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES-----------KGF---- 240
           DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES           K F    
Sbjct: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGESPEIPCYLSEEAKDFLEKC 240

Query: 241 ------------------------CRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300
                                   CRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE
Sbjct: 241 LKRNPSERWTANELMNHPFLKELNCRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300

Query: 301 LVRSDGWEAAEAEEQIRRL-------------------RKEEGEKNGGADEPEVKKCSNY 360
           LVRSDGWEAAEAEEQIRRL                   RKEEGEKNGGADEPEVKKCSNY
Sbjct: 301 LVRSDGWEAAEAEEQIRRLWMISGEPRWEEDENWITIRRKEEGEKNGGADEPEVKKCSNY 360

Query: 361 SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSFH 377
           SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSFH
Sbjct: 361 SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSFH 420

BLAST of Pay0016288 vs. NCBI nr
Match: KAA0034806.1 (mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 719.9 bits (1857), Expect = 1.1e-203
Identity = 373/434 (85.94%), Postives = 374/434 (86.18%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY
Sbjct: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60

Query: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120
           RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS
Sbjct: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120

Query: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180
           KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS
Sbjct: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180

Query: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES-----------KGF---- 240
           DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES           K F    
Sbjct: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGESPEIPCYLSEEAKDFLEKC 240

Query: 241 ------------------------CRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300
                                   CRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE
Sbjct: 241 LKRNPSERWTANELMNHPFLKELNCRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300

Query: 301 LVRSDGWEAAEAEEQIRRL-------------------RKEEGEKNGGADEPEVKKCSNY 360
           LVRSD WEAAEAEEQIRRL                   RKEEGEKNGGADEPEVKKCSNY
Sbjct: 301 LVRSDCWEAAEAEEQIRRLWTISGEPRWEEDENWITIRRKEEGEKNGGADEPEVKKCSNY 360

Query: 361 SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSFH 377
           SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNI+SLVNNNNPLSFH
Sbjct: 361 SKNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIVSLVNNNNPLSFH 420

BLAST of Pay0016288 vs. NCBI nr
Match: XP_004151935.1 (mitogen-activated protein kinase kinase kinase 18 [Cucumis sativus] >KGN63238.1 hypothetical protein Csa_022046 [Cucumis sativus])

HSP 1 Score: 664.8 bits (1714), Expect = 4.3e-187
Identity = 352/435 (80.92%), Postives = 359/435 (82.53%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           MDWTRGHVIGHGSSATVFLATDSPS  VFAVKTAQLS SQSLRKEQQFLSSLASPYIVSY
Sbjct: 1   MDWTRGHVIGHGSSATVFLATDSPSRHVFAVKTAQLSHSQSLRKEQQFLSSLASPYIVSY 60

Query: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120
           RGFEV REQSGV MFNLFMEYLPNGSLADTIRRRGGQRLDEATI IYTRQ+LMGLQYIHS
Sbjct: 61  RGFEVSREQSGVTMFNLFMEYLPNGSLADTIRRRGGQRLDEATIVIYTRQILMGLQYIHS 120

Query: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180
           KGIVHCDIKARNILIGLDGEAKLADFGCAK ATSQTDPI GTPLFMAPEVARGEHQGFPS
Sbjct: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKRATSQTDPICGTPLFMAPEVARGEHQGFPS 180

Query: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES-----------KGF---- 240
           DIWSIGCTIIEM +GGGSPWPKTTDDTDPISALYRIGYSGES           K F    
Sbjct: 181 DIWSIGCTIIEMASGGGSPWPKTTDDTDPISALYRIGYSGESPEIPCYLSEEAKDFLEKC 240

Query: 241 ------------------------CRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300
                                   CRR  KTEEVHSESPTSILEQGIWRSIEESEIRGRE
Sbjct: 241 LKRNPSERWTASELMNHPFLRELNCRREWKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300

Query: 301 LVRSDGWEAAEAEEQIRRL-------------------RKEEGEKNGGADEPEVKKCSNY 360
           LVRS+GWEAA AEEQIRRL                   RKEEGEKNGGADE EVKKCSNY
Sbjct: 301 LVRSNGWEAAAAEEQIRRLWMISGEPRWEEDENWITIRRKEEGEKNGGADESEVKKCSNY 360

Query: 361 S-KNNNNNSNWGEKKRGSGGEEWLEMELGNISCGISRNDLGDYSCRNIISLVNNNNPLSF 377
           S  NNNNNSNWG+KKRGSGG+EWL+MELGNISC ISRNDLGDYSCRN+ISLVNNN PLSF
Sbjct: 361 SNNNNNNNSNWGDKKRGSGGKEWLQMELGNISCRISRNDLGDYSCRNVISLVNNNYPLSF 420

BLAST of Pay0016288 vs. NCBI nr
Match: XP_038901954.1 (mitogen-activated protein kinase kinase kinase 18-like [Benincasa hispida])

HSP 1 Score: 523.5 bits (1347), Expect = 1.5e-144
Identity = 296/438 (67.58%), Postives = 315/438 (71.92%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           MDWTRGHVIGHGSSATVFLAT SP  DVFAVK+A+LS+SQSL+KEQQFLS L SPYIVSY
Sbjct: 1   MDWTRGHVIGHGSSATVFLATASPIRDVFAVKSAELSRSQSLQKEQQFLSFLVSPYIVSY 60

Query: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120
           RGFEV REQ+GVMMFNLFMEY PNGSLAD IRR GG+RLDEA I IYTRQLLMGLQYIHS
Sbjct: 61  RGFEVSREQNGVMMFNLFMEYFPNGSLADVIRRCGGRRLDEARIGIYTRQLLMGLQYIHS 120

Query: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180
           KG+VHCDIKARNILIG DG AKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGF +
Sbjct: 121 KGLVHCDIKARNILIGRDGVAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFAA 180

Query: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES-----------KGFCRR- 240
           DIWSIGCTIIEM TGGGSPWPKTTDDTDPIS LYRIGYS ES           K F  + 
Sbjct: 181 DIWSIGCTIIEMATGGGSPWPKTTDDTDPISTLYRIGYSEESPEIPCFLSEEAKDFLEKC 240

Query: 241 ---------------------------PCKTEEVHSESPTSILEQGIWRSIEESEIRGRE 300
                                      P K +EVH +SPTSILEQGIWRSIEESEIR  +
Sbjct: 241 LKGNPSERWTANQLVNHPFLRELNSATPRKIQEVHLDSPTSILEQGIWRSIEESEIRESK 300

Query: 301 LVRSDGWEAAEAEEQIRRLRKEEGEKNGGADE-----------------PEVKKCSNYSK 360
           L++     AA AEEQIRRL    GE     DE                  EVKKCS    
Sbjct: 301 LIK----PAAAAEEQIRRLWMVSGEPRWRDDENWITIRRKDTEKSREVGEEVKKCS---- 360

Query: 361 NNNNNSNWGEKKRGSGGEEWLEMEL----GNISCGISRNDLGDYSCRN--IISLVNNNNP 377
               N+ W EK+R SGG+EWLEMEL    GNI C IS     ++SCRN  IISLVNNNNP
Sbjct: 361 ----NNCWSEKQRDSGGKEWLEMELRDKNGNIICRIS-----NHSCRNNIIISLVNNNNP 420

BLAST of Pay0016288 vs. NCBI nr
Match: XP_022986310.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima])

HSP 1 Score: 515.8 bits (1327), Expect = 3.2e-142
Identity = 286/450 (63.56%), Postives = 311/450 (69.11%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           MDWTR HVIGHGSSATVFLATDSPSGDVFAVK+A++S SQSL+ EQQFLSSLASPY+VSY
Sbjct: 1   MDWTRRHVIGHGSSATVFLATDSPSGDVFAVKSAEISHSQSLQTEQQFLSSLASPYVVSY 60

Query: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120
           +G+EVRREQSGV+MFNLFMEYLPNGSLAD IRRRGG+RLDEA I IYTRQLL GLQYIHS
Sbjct: 61  KGYEVRREQSGVVMFNLFMEYLPNGSLADVIRRRGGRRLDEAAIGIYTRQLLQGLQYIHS 120

Query: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180
           KG+VHCDIK+RN+LIG DGEAKLADFGCAKWA SQTD IGGTPLFMAPEVARGEHQG PS
Sbjct: 121 KGLVHCDIKSRNVLIGRDGEAKLADFGCAKWA-SQTDSIGGTPLFMAPEVARGEHQGLPS 180

Query: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES------------------ 240
           DIWSIGC++IEM TGGGSPWPKTT D+DPISALYRIGYSGES                  
Sbjct: 181 DIWSIGCSVIEMATGGGSPWPKTTSDSDPISALYRIGYSGESPEIPSFLSHKAKDFLEKC 240

Query: 241 --------------------KGFCRRPCKTEEVHSESPTSILEQGIWRSIEESEIRGREL 300
                               + F   P K  EVHS SPTSILEQG+WRSIEESEIR  + 
Sbjct: 241 LRMNPNERWTANQLLNHPFVREFNSSPKKIHEVHSNSPTSILEQGVWRSIEESEIREAKS 300

Query: 301 VRSDGWEAAEAEEQIRRLRKEEGEKNGGADE-------------------------PEVK 360
            R + W    A EQIRRL    GE +   DE                          EVK
Sbjct: 301 CRPNSW----AAEQIRRLWMASGEPSWRDDETWITIRRQDIGKSKEVGDEGDTIGGSEVK 360

Query: 361 KCSNYSKNNNNNSNWGEKKRGSGGEEWLEMEL----GNISCGISRNDLGDYSCRNIISLV 377
            CS        N+ WGEK+RG GG+EW E E     G  SC ISRNDL D+ C+NIISLV
Sbjct: 361 GCS--------NNCWGEKQRGGGGKEWSETEFTQNNGGFSCRISRNDLADHRCKNIISLV 420

BLAST of Pay0016288 vs. TAIR 10
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17 )

HSP 1 Score: 268.1 bits (684), Expect = 1.1e-71
Identity = 139/306 (45.42%), Postives = 187/306 (61.11%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           M+WTRG ++G GS+ATV+ A    S ++ AVK++++ +S+ L++E + LSSL+SPY++ Y
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120
           RG E +RE +GV+M+NL MEY P G+L D   + GG R+DE  +  YTR +L GL+YIHS
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGG-RVDETRVVKYTRDILKGLEYIHS 120

Query: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTD-PIGGTPLFMAPEVARGEHQGFP 180
           KGIVHCD+K  N++I   GEAK+ADFGCAK      + P+ GTP FMAPEVARGE QG  
Sbjct: 121 KGIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKE 180

Query: 181 SDIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSG-----------ESKGFCRR 240
           SDIW++GCT+IEM T G  PW K     DP+S LYR+GYS            E+K F  +
Sbjct: 181 SDIWAVGCTMIEMVT-GSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEK 240

Query: 241 PCKTEE----------------------------VHSESPTSILEQGIWRSIEESEIRGR 267
             K E                             + S SPTS+ +Q  WRS+EE E    
Sbjct: 241 CLKREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEEEEET 300

BLAST of Pay0016288 vs. TAIR 10
Match: AT1G05100.1 (mitogen-activated protein kinase kinase kinase 18 )

HSP 1 Score: 255.8 bits (652), Expect = 5.6e-68
Identity = 144/316 (45.57%), Postives = 185/316 (58.54%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           M+WTRG  +G GS+ATV  AT   SG+  AVK+A+  +S+ L++E + LSSL SPY++ Y
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  RGFEVRRE----QSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQ 120
           RG E+ RE          ++L MEY P G+L D   + GG  +DEA +  YTRQ+L+GL+
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGG-FIDEARVVKYTRQILLGLE 120

Query: 121 YIH-SKGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQ-TDPIGGTPLFMAPEVARGE 180
           YIH SKGI HCDIK  N+L+G +GEAK+ADFGCAKW   + T+P+ GTP FMAPE ARGE
Sbjct: 121 YIHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPVRGTPAFMAPEAARGE 180

Query: 181 HQGFPSDIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGE-----------SK 240
            QG  SDIW++GCT+IEM T G  PW    D TDP+S LYR+GY GE           +K
Sbjct: 181 RQGKESDIWAVGCTVIEMVT-GSQPW-IGADFTDPVSVLYRVGYLGELPELPCSLTEQAK 240

Query: 241 GFCRRPCKTEEVH-------------------------SESPTSILEQGIWRSIEESEIR 275
            F  +  K E                            + SPTS+ +Q  WRS+EE    
Sbjct: 241 DFLGKCLKKEATERWTASQLLNHPFLVNKEPELVTGLVTNSPTSVTDQMFWRSVEEEVSE 300

BLAST of Pay0016288 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 230.3 bits (586), Expect = 2.5e-60
Identity = 116/222 (52.25%), Postives = 160/222 (72.07%), Query Frame = 0

Query: 1   MDWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSY 60
           ++WTRG +IG GS+ATV +A  S SG++FAVK+A LS S  L+KEQ  LS+L+SP++V Y
Sbjct: 3   INWTRGPIIGRGSTATVSIAISS-SGELFAVKSADLSSSSLLQKEQSILSTLSSPHMVKY 62

Query: 61  RGFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHS 120
            G  + RE +G +++N+ MEY+  G+L D I+  GG +L E  I  YTRQ+L GL Y+H 
Sbjct: 63  IGTGLTRESNG-LVYNILMEYVSGGNLHDLIKNSGG-KLPEPEIRSYTRQILNGLVYLHE 122

Query: 121 KGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPIGGTPLFMAPEVARGEHQGFPS 180
           +GIVHCD+K+ N+L+  +G  K+AD GCAK  +       GTP FMAPEVARGE Q FP+
Sbjct: 123 RGIVHCDLKSHNVLVEENGVLKIADMGCAK--SVDKSEFSGTPAFMAPEVARGEEQRFPA 182

Query: 181 DIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES 223
           D+W++GCT+IEM T G SPWP+     D ++A+Y+IG+SGES
Sbjct: 183 DVWALGCTMIEMMT-GSSPWPEL---NDVVAAMYKIGFSGES 215

BLAST of Pay0016288 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 225.7 bits (574), Expect = 6.2e-59
Identity = 117/223 (52.47%), Postives = 156/223 (69.96%), Query Frame = 0

Query: 2   DWTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQLSQSQSLRKEQQFLSSLASPYIVSYR 61
           +W RG +IG GS+ATV L   + SGD FAVK+A+ S S  L++EQ  LS L+SPYIV Y 
Sbjct: 5   NWIRGPIIGRGSTATVSLGITN-SGDFFAVKSAEFSSSAFLQREQSILSKLSSPYIVKYI 64

Query: 62  GFEVRREQSGVMMFNLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGLQYIHSK 121
           G  V +E +  +M+NL MEY+  GSL D I+  GG +L E  I  YTRQ+L GL Y+H +
Sbjct: 65  GSNVTKE-NDKLMYNLLMEYVSGGSLHDLIKNSGG-KLPEPLIRSYTRQILKGLMYLHDQ 124

Query: 122 GIVHCDIKARNILIGLDGE-AKLADFGCAKWATSQTD-PIGGTPLFMAPEVARGEHQGFP 181
           GIVHCD+K++N++IG  GE AK+ D GCAK      +    GTP FM+PEVARGE Q FP
Sbjct: 125 GIVHCDVKSQNVMIG--GEIAKIVDLGCAKTVEENENLEFSGTPAFMSPEVARGEEQSFP 184

Query: 182 SDIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGES 223
           +D+W++GCT+IEM T G SPWP+     D ++A+Y+IG++GES
Sbjct: 185 ADVWALGCTVIEMAT-GSSPWPEL---NDVVAAIYKIGFTGES 218

BLAST of Pay0016288 vs. TAIR 10
Match: AT1G07150.1 (mitogen-activated protein kinase kinase kinase 13 )

HSP 1 Score: 164.5 bits (415), Expect = 1.7e-40
Identity = 106/242 (43.80%), Postives = 135/242 (55.79%), Query Frame = 0

Query: 3   WTRGHVIGHGSSATVFLATDSPSGDVFAVKTAQL-----SQSQSLRKEQQFLSSL-ASPY 62
           W RG  IG G    V  A    +G+VFAVK+  L     +QS+SL  E     SL   PY
Sbjct: 23  WVRGACIGRGCFGAVSTAISKTNGEVFAVKSVDLATSLPTQSESLENEISVFRSLKPHPY 82

Query: 63  IVSYRGFEVRREQSGVMMF-NLFMEYLPNGSLADTIRRRGGQRLDEATIAIYTRQLLMGL 122
           IV + G  V +E  G   F NL++EYLPNG +A    R GG+  DE  +  YT  L+  L
Sbjct: 83  IVKFLGDGVSKE--GTTTFRNLYLEYLPNGDVAS--HRAGGKIEDETLLQRYTACLVSAL 142

Query: 123 QYIHSKGIVHCDIKARNILIGLDGEAKLADFGCAKWATSQTDPI--GGTPLFMAPEVARG 182
           +++HS+G VHCD+KARNIL+      KLADFG A    +    I   G+PL+MAPEV R 
Sbjct: 143 RHVHSQGFVHCDVKARNILVSQSSMVKLADFGSAFRIHTPRALITPRGSPLWMAPEVIRR 202

Query: 183 EHQGFPSDIWSIGCTIIEMGTGGGSPWPKTTDDTDPISALYRIGYSGESKGFCRRPCKTE 236
           E+QG  SD+WS+GCTIIEM TG      K   +   I +L RI +S E   F   P K  
Sbjct: 203 EYQGPESDVWSLGCTIIEMFTG------KPAWEDHGIDSLSRISFSDELPVF---PSKLS 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O808881.5e-7045.42Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q9ZVP57.8e-6745.57Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
Q9SND62.1e-3539.66Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
Q405412.7e-3534.63Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
Q9FZ363.0e-3436.36Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A5D3CC811.9e-20485.94Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuw... [more]
A0A1S3C2N91.9e-20485.94mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN... [more]
A0A5A7SY505.4e-20485.94Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuw... [more]
A0A0A0LN492.1e-18780.92Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G416... [more]
A0A6J1JDQ61.5e-14263.56mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
XP_008455899.13.8e-20485.94PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo] ... [more]
KAA0034806.11.1e-20385.94mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa][more]
XP_004151935.14.3e-18780.92mitogen-activated protein kinase kinase kinase 18 [Cucumis sativus] >KGN63238.1 ... [more]
XP_038901954.11.5e-14467.58mitogen-activated protein kinase kinase kinase 18-like [Benincasa hispida][more]
XP_022986310.13.2e-14263.56mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G32510.11.1e-7145.42mitogen-activated protein kinase kinase kinase 17 [more]
AT1G05100.15.6e-6845.57mitogen-activated protein kinase kinase kinase 18 [more]
AT4G26890.12.5e-6052.25mitogen-activated protein kinase kinase kinase 16 [more]
AT5G55090.16.2e-5952.47mitogen-activated protein kinase kinase kinase 15 [more]
AT1G07150.11.7e-4043.80mitogen-activated protein kinase kinase kinase 13 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPRINTSPR00109TYRKINASEcoord: 79..92
score: 37.33
coord: 117..135
score: 43.5
coord: 180..202
score: 41.28
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 3..369
e-value: 1.3E-49
score: 180.8
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 5..207
e-value: 8.6E-46
score: 156.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 3..250
score: 35.919453
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 77..298
e-value: 2.5E-45
score: 157.0
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 1..76
e-value: 9.5E-11
score: 44.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 280..299
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 300..315
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 280..319
NoneNo IPR availablePANTHERPTHR48011:SF45MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 18coord: 2..222
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 2..222
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 2..222
e-value: 6.45127E-101
score: 297.51
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 9..32
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 123..135
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 2..204

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0016288.1Pay0016288.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity