Homology
BLAST of Pay0015555 vs. ExPASy Swiss-Prot
Match:
Q3MIT2 (tRNA pseudouridine synthase Pus10 OS=Homo sapiens OX=9606 GN=PUS10 PE=1 SV=1)
HSP 1 Score: 247.3 bits (630), Expect = 3.9e-64
Identity = 160/440 (36.36%), Postives = 249/440 (56.59%), Query Frame = 0
Query: 83 DNDRNTCCLCLGILQFSYVDDKGDFIKCKGVDDLAVSISEMVKKEGHQIDSFSLEVSVPS 142
+++ N C +CLGILQ + DFIK + + V+ G + S VS P
Sbjct: 103 NSNLNVCNVCLGILQEFC---EKDFIK---------KVCQKVEASGFEFTSLVFSVSFPP 162
Query: 143 IILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDALKICITKPL--ETLLDCKSSSSG 202
+ E WL +K++ G + G+ + L K+A K IT PL E L S
Sbjct: 163 QLSVREHAAWLLVKQEMGKQSLSLGRDDIVQL--KEAYK-WITHPLFSEELGVPIDGKSL 222
Query: 203 LRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSDISNCNVNSVADDCINPSQEV 262
+ + ++HP+ D FK K S +N + ++ + ++
Sbjct: 223 FEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEE--DFLKQF 282
Query: 263 IFPQETIKEPCH-LALTCYRTSIYMFGRYIKYSRNVSQTRWVIGEERMGEASVEEILGNN 322
P + K C L + C ++++ GRY KYSRN+ QT W+I ER E+SVEE++ ++
Sbjct: 283 PCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDH 342
Query: 323 ILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNA-RLLPSEMIINEIKSKINSPET 382
+L + +S+ F ++GRED+DVR LG+GRPF +E+ N R+ + I E++ KIN+
Sbjct: 343 LLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSN 402
Query: 383 KLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDGDFLSISSLKDLKILQRT 442
K + VR+L++V E ++EGE EK K Y+AL+WT++ ++ D ++ +KDLKI Q+T
Sbjct: 403 K-IQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKT 462
Query: 443 PIRVLHRRSPLKREKIIHWMKIEKVAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIG 502
P+RVLHRR R ++IH+M+ + V E ++ LHL TQAGTYIKEFVHGD GRT P+IG
Sbjct: 463 PLRVLHRRPLAVRARVIHFMETQYVDE--HHFRLHLKTQAGTYIKEFVHGDFGRTKPNIG 522
Query: 503 SILSCRAEILQLDVTDIKMD 519
S+++ A+IL+LDV + +D
Sbjct: 523 SLMNVTADILELDVESVDVD 522
BLAST of Pay0015555 vs. ExPASy Swiss-Prot
Match:
Q9D3U0 (tRNA pseudouridine synthase Pus10 OS=Mus musculus OX=10090 GN=Pus10 PE=1 SV=1)
HSP 1 Score: 240.4 bits (612), Expect = 4.7e-62
Identity = 164/453 (36.20%), Postives = 247/453 (54.53%), Query Frame = 0
Query: 76 DKEAIEDDNDRNTCCLCLGILQFSYVDDKGDFIKCKGVDDLAVSISEMVKKEGHQIDSFS 135
D+ E + C +CLGILQ +KG K + + V+ G + S
Sbjct: 94 DESMAEKPSKLRVCNVCLGILQ--EFCEKGFITK----------VCQKVEASGFEFTSVV 153
Query: 136 LEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDALKICITKPL--ETLLD 195
L VS P + E WL +K++ G + G+ + L K+A K IT PL E L
Sbjct: 154 LSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRNDVVQL--KEAYK-WITHPLFSEELGV 213
Query: 196 CKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSDISNCNVNSVADDC 255
S + + ++HP+ + + +D FK K ++ S + V
Sbjct: 214 PTDGKSLFEVSVVFAHPETAEDCHFLGEVCRDCFKPAK----NKQSVFTRMAVLK----A 273
Query: 256 INPSQEVIF------PQETIKEPCH-LALTCYRTSIYMFGRYIKYSRNVSQTRWVIGEER 315
++ +E F P + K C L + C ++++ GRY KYSRN+ QT W+I ER
Sbjct: 274 LSKIKEEDFLGQFPCPPNSPKTVCTVLEVECTHGAVFVAGRYNKYSRNLPQTPWIIDGER 333
Query: 316 MGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNA-RLLPSEMI 375
E+SVEE++ +++L R +S+ F ++GRED+DVR LG+GRPF VE+ N R+ +
Sbjct: 334 KMESSVEELISDHLLAVFRAESFNFSSSGREDVDVRTLGNGRPFAVELLNPHRVHFTSQE 393
Query: 376 INEIKSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDGDFLS 435
+ E++ IN K + VR+L++V E ++EGE EK K Y+AL+WT+R ++ D
Sbjct: 394 MKELQQTINKSSDK-IQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNRAIQKKDIGF 453
Query: 436 ISSLKDLKILQRTPIRVLHRRSPLKREKIIHWMKIEKVAESSQYYLLHLCTQAGTYIKEF 495
+ LKDLKI Q+TP+RVLHRR R + IH MK + E ++ LHL TQAGTYIKEF
Sbjct: 454 LDDLKDLKIDQKTPLRVLHRRPLAVRTRAIHSMKTHYLDE--HHFRLHLKTQAGTYIKEF 513
Query: 496 VHGDLGRTHPSIGSILSCRAEILQLDVTDIKMD 519
VHGD GRT P++GS+++ A+IL+LDV + +D
Sbjct: 514 VHGDFGRTKPNLGSLMNVTADILELDVESVDVD 520
BLAST of Pay0015555 vs. ExPASy Swiss-Prot
Match:
Q6ING2 (tRNA pseudouridine synthase Pus10 OS=Xenopus laevis OX=8355 GN=pus10 PE=2 SV=1)
HSP 1 Score: 233.4 bits (594), Expect = 5.8e-60
Identity = 151/435 (34.71%), Postives = 236/435 (54.25%), Query Frame = 0
Query: 89 CCLCLGILQFSYVDDKGDFIKCKGVDDLAVSISEMVKKEGHQIDSFSLEVSVPSIILENE 148
C LCLGILQ F + + ++ + V I+ V +++ F L +S+P+ + E
Sbjct: 96 CPLCLGILQ--------QFCEPEFIEKVFVKINSAV----YELKDFVLSISLPAQLSVRE 155
Query: 149 KRVWLYLKRKYGSEQWFQGKPPPICLSAKDALKICITKPLETLLDCKSSSSGL-RIRLTY 208
WL K++ G K + L K+A K I L L ++ S L + + +
Sbjct: 156 HSAWLQAKQEMGKHGHSLDKTDIVQL--KEAYKWIIHPLLSDQLGIQADSKSLFEVAVVF 215
Query: 209 SHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSDISNCNVNSVADDCINPSQEV--IFPQE 268
+HP+ +D FK K ++ S + V + V FP
Sbjct: 216 THPETDGDCHFLATVCRDCFKPTK----NKQSVFTRMAVVKALEKIKEEDFRVQFPFPPS 275
Query: 269 TIKEPCHLA-LTCYRTSIYMFGRYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFC 328
+ + C + + C + +++ GRY KYSRN+ QT W+I ER E+SVEE++ ++++
Sbjct: 276 SPETTCEVVDIQCNHSPVFVAGRYNKYSRNLPQTPWIIDGERKIESSVEELITDHLVAAF 335
Query: 329 RGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLLP-SEMIINEIKSKINSPETKLVGV 388
R S+ F ++GRED+DVR LG GRPF +E+ N + + I ++ KIN+ + + V
Sbjct: 336 RSSSFNFSSSGREDVDVRTLGKGRPFAIELLNPHKVQFTGQEIKALQQKINTSDK--IKV 395
Query: 389 RNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDGDFLSISSLKDLKILQRTPIRVL 448
R+L++V E A ++EGE EK K Y AL+W + + + D + L++L I Q+TP+RVL
Sbjct: 396 RDLQIVSREAVAHMKEGEEEKTKCYCALIWIEKMVRNEDLQLLDGLEELTIAQKTPLRVL 455
Query: 449 HRRSPLKREKIIHWMKIEKVAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSC 508
HRR R + IH M+ E V E ++ L+L TQAGTYIKEFVHGD GRT P++GSI+
Sbjct: 456 HRRPLASRSRTIHTMRTEYVDE--HHFRLYLKTQAGTYIKEFVHGDFGRTKPNVGSIMET 508
Query: 509 RAEILQLDVTDIKMD 519
A+IL+LDV + +D
Sbjct: 516 NADILELDVESVDVD 508
BLAST of Pay0015555 vs. ExPASy Swiss-Prot
Match:
A1RX33 (tRNA pseudouridine synthase Pus10 OS=Thermofilum pendens (strain DSM 2475 / Hrk 5) OX=368408 GN=pus10 PE=3 SV=1)
HSP 1 Score: 150.6 bits (379), Expect = 4.9e-35
Identity = 88/245 (35.92%), Postives = 136/245 (55.51%), Query Frame = 0
Query: 274 ALTCYRTSIYMFGRYIKYSRNVSQTRWVIGEERMG-EASVEEILGNNILPFCRGDSYKFH 333
A+ + ++++GRY KY+R + Q W +ER+ S+EE++ L + KFH
Sbjct: 200 AIELHPAPVFVYGRYRKYARGLPQNPWPQPDERIKFNTSIEELIVKPALELFEAEKAKFH 259
Query: 334 AAGREDLDVRMLGSGRPFLVEIQNARLLPSEMIINEIKSKINSPETKLVGVRNLKVVGSE 393
AAGRED+DVR LG+GRPF++EI+ R + + + KINS L+ V +L +
Sbjct: 260 AAGREDIDVRTLGTGRPFVLEIKKPR--KRNIDLKVLAEKINSGAGGLIEVLDLAYTDRK 319
Query: 394 GWALVQEGEAEKQKQYAALVWTSRPLEDGDFLSISSL-KDLKILQRTPIRVLHRRSPLKR 453
++ + +K Y A V +P++D IS + + I QRTP RVLHRR R
Sbjct: 320 TIKKLKSLASIAKKAYVARVKFEKPVDDEKLAEISKVFSNAVINQRTPTRVLHRRVDKLR 379
Query: 454 EKIIHWMKIEKVAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLD 513
+KI++ ++ K+++ + L TQ G Y+KEF+HGD GRT PSI L ++LD
Sbjct: 380 KKIVYRLEARKISQDEVEFYLE--TQGGFYVKEFIHGDNGRTTPSIAEFLGNNVLSIELD 439
Query: 514 VTDIK 517
V I+
Sbjct: 440 VVSIE 440
BLAST of Pay0015555 vs. ExPASy Swiss-Prot
Match:
Q9VPK7 (Putative tRNA pseudouridine synthase Pus10 OS=Drosophila melanogaster OX=7227 GN=Pus10 PE=2 SV=2)
HSP 1 Score: 149.4 bits (376), Expect = 1.1e-34
Identity = 118/397 (29.72%), Postives = 193/397 (48.61%), Query Frame = 0
Query: 136 LEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDALKICITKPLETLLDCK 195
L + +P + + +W L+R++G + PP + + K+A+K+ + + L
Sbjct: 105 LAICLPMSLQLRQLAMWFALRRRFG-PSIDENNPPDVPI--KEAVKLILHPIICEKLAKV 164
Query: 196 SSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSDISNCNVNSVADDCIN 255
++GL I + +H +K N++ F + + +IS + +
Sbjct: 165 YDANGLMINIDLTHSLEGEEVEKLVKLNKEAFPAK--ASHQKRIEIS----RGLLEKQYQ 224
Query: 256 PSQ------EVIF--PQETIKEPCHL-ALTCYRTSIYMFGRYIKYSRNVSQTRWVIGEER 315
PS+ E F P ++E L ++ I + GRY K SR +S T W++ +R
Sbjct: 225 PSKVKAETYEKYFQIPCSNVEESIKLTSIDLQGPLICVAGRYRKLSRELSHTPWILNGQR 284
Query: 316 MGEASVEEILGNNILPFCRGDSYK--FHAAGREDLDVRMLGSGRPFLVEIQNAR---LLP 375
+ E S+EEI+ ++ P K F ++GRED+DVR LG GRPF++EI NA+ L
Sbjct: 285 LMEDSIEEIIIRHVGPHFTEKLGKITFMSSGREDVDVRCLGKGRPFVLEIPNAKRSCLTK 344
Query: 376 SEMIINEIKSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLEDG 435
E E + +V + +L+VV E ++ GE +K+K Y AL P+
Sbjct: 345 DEAFAMERAVDASG----MVSIHHLQVVPREELTHIKTGEEQKKKFYRALCALQEPVSVK 404
Query: 436 DFLSISSLKDLKILQRTPIRVLHRRSPLKREKIIHWMKIEKVAESSQYYLLHLCTQAGTY 495
+ I Q+TPIRVLHRR R + I+ + + + + ++ + TQAGTY
Sbjct: 405 ILEQLQISASFNIQQKTPIRVLHRRPLHTRPRTIYSVTAKVHRGNPKALIIDIVTQAGTY 464
Query: 496 IKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMD 519
IKE VHG+ GRT PS+ SI+ +I LDV I +D
Sbjct: 465 IKELVHGEFGRTTPSLSSIIGKPMDIQALDVVGIDLD 488
BLAST of Pay0015555 vs. ExPASy TrEMBL
Match:
A0A1S3BUF4 (tRNA pseudouridine(55) synthase OS=Cucumis melo OX=3656 GN=LOC103493791 PE=3 SV=1)
HSP 1 Score: 1046.2 bits (2704), Expect = 4.6e-302
Identity = 517/523 (98.85%), Postives = 521/523 (99.62%), Query Frame = 0
Query: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE
Sbjct: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
Query: 61 RHNYSFSLSAPILGSDKEAIEDDNDRNTCCLCLGILQFSYVDDKGDFIKCKGVDDLAVSI 120
RHNYSFSLSAPILGSD EAIEDDNDRNTCCLCLGILQFSY+DDKGDFIKCKGVDDLAVSI
Sbjct: 61 RHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
Query: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240
KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240
Query: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300
ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW
Sbjct: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300
Query: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL
Sbjct: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
Query: 361 PSEMIINEIKSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
PSEMIINEI+SKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED
Sbjct: 361 PSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
Query: 421 GDFLSISSLKDLKILQRTPIRVLHRRSPLKREKIIHWMKIEKVAESSQYYLLHLCTQAGT 480
DFLSISSLKDLKILQRTPIRVLHRRSPL+REKIIHWMKIEKVA+SSQYYLLHLCTQAGT
Sbjct: 421 DDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAKSSQYYLLHLCTQAGT 480
Query: 481 YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 524
YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE
Sbjct: 481 YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 523
BLAST of Pay0015555 vs. ExPASy TrEMBL
Match:
A0A5D3D906 (tRNA pseudouridine(55) synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00830 PE=3 SV=1)
HSP 1 Score: 1036.2 bits (2678), Expect = 4.7e-299
Identity = 516/537 (96.09%), Postives = 521/537 (97.02%), Query Frame = 0
Query: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE
Sbjct: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
Query: 61 RHNYSFSLSAPILGSDKEAIEDDNDRNTCCLCLGILQFSYVDDKGDFIKCKGVDDLAVSI 120
RHNYSFSLSAPILGSD EAIEDDNDRNTCCLCLGILQFSY+DDKGDFIKCKGVDDLAVSI
Sbjct: 61 RHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
Query: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKI-------- 240
KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKI
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKISNDCSLET 240
Query: 241 ------IGSDEPSDISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFG 300
+GSDEPSDISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFG
Sbjct: 241 IDDSIAVGSDEPSDISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFG 300
Query: 301 RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS 360
RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS
Sbjct: 301 RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS 360
Query: 361 GRPFLVEIQNARLLPSEMIINEIKSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQK 420
GRPFLVEIQNARLLPSEMIINEI+SKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQK
Sbjct: 361 GRPFLVEIQNARLLPSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQK 420
Query: 421 QYAALVWTSRPLEDGDFLSISSLKDLKILQRTPIRVLHRRSPLKREKIIHWMKIEKVAES 480
QYAALVWTSRPLED DFLSISSLKDLKILQRTPIRVLHRRSPL+REKIIHWMKIEKVA+S
Sbjct: 421 QYAALVWTSRPLEDDDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAKS 480
Query: 481 SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 524
SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE
Sbjct: 481 SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 537
BLAST of Pay0015555 vs. ExPASy TrEMBL
Match:
A0A5A7V921 (tRNA pseudouridine(55) synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003460 PE=3 SV=1)
HSP 1 Score: 1030.4 bits (2663), Expect = 2.6e-297
Identity = 517/553 (93.49%), Postives = 521/553 (94.21%), Query Frame = 0
Query: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE
Sbjct: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
Query: 61 RHNYSFSLSAPILGSDKEAIEDDNDRNTCCLCLGILQFSYVDDKGDFIKCKGVDDLAVSI 120
RHNYSFSLSAPILGSD EAIEDDNDRNTCCLCLGILQFSY+DDKGDFIKCKGVDDLAVSI
Sbjct: 61 RHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
Query: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240
KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240
Query: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300
ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW
Sbjct: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300
Query: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL
Sbjct: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
Query: 361 PSEMIINEIKSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
PSEMIINEI+SKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED
Sbjct: 361 PSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
Query: 421 GDFLSISSLKDLKILQRTPIRVLHRRSPLKREKIIHW----------------------- 480
DFLSISSLKDLKILQRTPIRVLHRRSPL+REKIIHW
Sbjct: 421 DDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWLESFFNCTVKLQSTHASTKVTDN 480
Query: 481 -------MKIEKVAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQL 524
MKIEKVA+SSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQL
Sbjct: 481 VLLFHYRMKIEKVAKSSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQL 540
BLAST of Pay0015555 vs. ExPASy TrEMBL
Match:
A0A0A0L378 (tRNA pseudouridine(55) synthase OS=Cucumis sativus OX=3659 GN=Csa_4G653400 PE=3 SV=1)
HSP 1 Score: 1010.0 bits (2610), Expect = 3.6e-291
Identity = 500/523 (95.60%), Postives = 509/523 (97.32%), Query Frame = 0
Query: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
MANEQDEVQANPEAT LALTSVTQEAISPGDKLQ PSYA KDLLSIGVCIPCIIRLSGIE
Sbjct: 1 MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE 60
Query: 61 RHNYSFSLSAPILGSDKEAIEDDNDRNTCCLCLGILQFSYVDDKGDFIKCKGVDDLAVSI 120
RH+YSFSLSAPILGSD EAI+D NDRNTCCLCLGILQFSY+DDKGDFIKCKGVDDLAVSI
Sbjct: 61 RHSYSFSLSAPILGSDNEAIDDANDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
Query: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240
KICITKPLETLLDCKSSSSGLRIRLTYSHPKASN NGNSIKRNQDGFKMRKI+GSDEPSD
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSD 240
Query: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300
ISNC NSVADDCINPSQEV+FPQETIKEPCHLALTCYRTSIY+ GRYIKYSRNVSQTRW
Sbjct: 241 ISNC--NSVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYSRNVSQTRW 300
Query: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL
Sbjct: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
Query: 361 PSEMIINEIKSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
PSEMIINEI+SKINS E KL+GVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED
Sbjct: 361 PSEMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
Query: 421 GDFLSISSLKDLKILQRTPIRVLHRRSPLKREKIIHWMKIEKVAESSQYYLLHLCTQAGT 480
D LSISSLKDLKILQRTPIRVLHRRSPL+REKIIHWMKIEKVAESSQYYLLHLCTQAGT
Sbjct: 421 ADLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGT 480
Query: 481 YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 524
YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHL E
Sbjct: 481 YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE 521
BLAST of Pay0015555 vs. ExPASy TrEMBL
Match:
E5GCD5 (tRNA pseudouridine(55) synthase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)
HSP 1 Score: 937.9 bits (2423), Expect = 1.8e-269
Identity = 480/537 (89.39%), Postives = 485/537 (90.32%), Query Frame = 0
Query: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE
Sbjct: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
Query: 61 RHNYSFSLSAPILGSDKEAIEDDNDRNTCCLCLGILQFSYVDDKGDFIKCKGVDDLAVSI 120
RHNYSFSLSAPILGSD EAIEDDNDRNTCCLCLGILQFSY+DDKGDFIKCKGVDDLAVSI
Sbjct: 61 RHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
Query: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKI-------- 240
KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKI
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKISNDCSLET 240
Query: 241 ------IGSDEPSDISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFG 300
+GSDEPSDISNCNVNSVADDCINPSQEVIFPQET
Sbjct: 241 IDDSIAVGSDEPSDISNCNVNSVADDCINPSQEVIFPQET-------------------- 300
Query: 301 RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS 360
YSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAA
Sbjct: 301 ----YSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAA------------ 360
Query: 361 GRPFLVEIQNARLLPSEMIINEIKSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQK 420
GRPFLVEIQNARLLPSEMIINEI+SKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQK
Sbjct: 361 GRPFLVEIQNARLLPSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQK 420
Query: 421 QYAALVWTSRPLEDGDFLSISSLKDLKILQRTPIRVLHRRSPLKREKIIHWMKIEKVAES 480
QYAALVWTSRPLED DFLSISSLKDLKILQRTPIRVLHRRSPL+REKIIHWMKIEKVA+S
Sbjct: 421 QYAALVWTSRPLEDDDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAKS 480
Query: 481 SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 524
SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE
Sbjct: 481 SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 501
BLAST of Pay0015555 vs. NCBI nr
Match:
XP_008452922.1 (PREDICTED: putative tRNA pseudouridine synthase Pus10 [Cucumis melo])
HSP 1 Score: 1046.2 bits (2704), Expect = 9.5e-302
Identity = 517/523 (98.85%), Postives = 521/523 (99.62%), Query Frame = 0
Query: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE
Sbjct: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
Query: 61 RHNYSFSLSAPILGSDKEAIEDDNDRNTCCLCLGILQFSYVDDKGDFIKCKGVDDLAVSI 120
RHNYSFSLSAPILGSD EAIEDDNDRNTCCLCLGILQFSY+DDKGDFIKCKGVDDLAVSI
Sbjct: 61 RHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
Query: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240
KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240
Query: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300
ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW
Sbjct: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300
Query: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL
Sbjct: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
Query: 361 PSEMIINEIKSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
PSEMIINEI+SKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED
Sbjct: 361 PSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
Query: 421 GDFLSISSLKDLKILQRTPIRVLHRRSPLKREKIIHWMKIEKVAESSQYYLLHLCTQAGT 480
DFLSISSLKDLKILQRTPIRVLHRRSPL+REKIIHWMKIEKVA+SSQYYLLHLCTQAGT
Sbjct: 421 DDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAKSSQYYLLHLCTQAGT 480
Query: 481 YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 524
YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE
Sbjct: 481 YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 523
BLAST of Pay0015555 vs. NCBI nr
Match:
TYK19969.1 (putative tRNA pseudouridine synthase Pus10 [Cucumis melo var. makuwa])
HSP 1 Score: 1036.2 bits (2678), Expect = 9.8e-299
Identity = 516/537 (96.09%), Postives = 521/537 (97.02%), Query Frame = 0
Query: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE
Sbjct: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
Query: 61 RHNYSFSLSAPILGSDKEAIEDDNDRNTCCLCLGILQFSYVDDKGDFIKCKGVDDLAVSI 120
RHNYSFSLSAPILGSD EAIEDDNDRNTCCLCLGILQFSY+DDKGDFIKCKGVDDLAVSI
Sbjct: 61 RHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
Query: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKI-------- 240
KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKI
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKISNDCSLET 240
Query: 241 ------IGSDEPSDISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFG 300
+GSDEPSDISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFG
Sbjct: 241 IDDSIAVGSDEPSDISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFG 300
Query: 301 RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS 360
RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS
Sbjct: 301 RYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGS 360
Query: 361 GRPFLVEIQNARLLPSEMIINEIKSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQK 420
GRPFLVEIQNARLLPSEMIINEI+SKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQK
Sbjct: 361 GRPFLVEIQNARLLPSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQK 420
Query: 421 QYAALVWTSRPLEDGDFLSISSLKDLKILQRTPIRVLHRRSPLKREKIIHWMKIEKVAES 480
QYAALVWTSRPLED DFLSISSLKDLKILQRTPIRVLHRRSPL+REKIIHWMKIEKVA+S
Sbjct: 421 QYAALVWTSRPLEDDDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAKS 480
Query: 481 SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 524
SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE
Sbjct: 481 SQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 537
BLAST of Pay0015555 vs. NCBI nr
Match:
KAA0064623.1 (putative tRNA pseudouridine synthase Pus10 [Cucumis melo var. makuwa])
HSP 1 Score: 1030.4 bits (2663), Expect = 5.4e-297
Identity = 517/553 (93.49%), Postives = 521/553 (94.21%), Query Frame = 0
Query: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE
Sbjct: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
Query: 61 RHNYSFSLSAPILGSDKEAIEDDNDRNTCCLCLGILQFSYVDDKGDFIKCKGVDDLAVSI 120
RHNYSFSLSAPILGSD EAIEDDNDRNTCCLCLGILQFSY+DDKGDFIKCKGVDDLAVSI
Sbjct: 61 RHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
Query: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240
KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240
Query: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300
ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW
Sbjct: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300
Query: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL
Sbjct: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
Query: 361 PSEMIINEIKSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
PSEMIINEI+SKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED
Sbjct: 361 PSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
Query: 421 GDFLSISSLKDLKILQRTPIRVLHRRSPLKREKIIHW----------------------- 480
DFLSISSLKDLKILQRTPIRVLHRRSPL+REKIIHW
Sbjct: 421 DDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWLESFFNCTVKLQSTHASTKVTDN 480
Query: 481 -------MKIEKVAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQL 524
MKIEKVA+SSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQL
Sbjct: 481 VLLFHYRMKIEKVAKSSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQL 540
BLAST of Pay0015555 vs. NCBI nr
Match:
XP_011654255.1 (putative tRNA pseudouridine synthase Pus10 [Cucumis sativus] >KGN55479.1 hypothetical protein Csa_012687 [Cucumis sativus])
HSP 1 Score: 1010.0 bits (2610), Expect = 7.5e-291
Identity = 500/523 (95.60%), Postives = 509/523 (97.32%), Query Frame = 0
Query: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDKLQFPSYAAKDLLSIGVCIPCIIRLSGIE 60
MANEQDEVQANPEAT LALTSVTQEAISPGDKLQ PSYA KDLLSIGVCIPCIIRLSGIE
Sbjct: 1 MANEQDEVQANPEATALALTSVTQEAISPGDKLQIPSYATKDLLSIGVCIPCIIRLSGIE 60
Query: 61 RHNYSFSLSAPILGSDKEAIEDDNDRNTCCLCLGILQFSYVDDKGDFIKCKGVDDLAVSI 120
RH+YSFSLSAPILGSD EAI+D NDRNTCCLCLGILQFSY+DDKGDFIKCKGVDDLAVSI
Sbjct: 61 RHSYSFSLSAPILGSDNEAIDDANDRNTCCLCLGILQFSYIDDKGDFIKCKGVDDLAVSI 120
Query: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL
Sbjct: 121 SEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICLSAKDAL 180
Query: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKIIGSDEPSD 240
KICITKPLETLLDCKSSSSGLRIRLTYSHPKASN NGNSIKRNQDGFKMRKI+GSDEPSD
Sbjct: 181 KICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKIVGSDEPSD 240
Query: 241 ISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYSRNVSQTRW 300
ISNC NSVADDCINPSQEV+FPQETIKEPCHLALTCYRTSIY+ GRYIKYSRNVSQTRW
Sbjct: 241 ISNC--NSVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYSRNVSQTRW 300
Query: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL
Sbjct: 301 VIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARLL 360
Query: 361 PSEMIINEIKSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
PSEMIINEI+SKINS E KL+GVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED
Sbjct: 361 PSEMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWTSRPLED 420
Query: 421 GDFLSISSLKDLKILQRTPIRVLHRRSPLKREKIIHWMKIEKVAESSQYYLLHLCTQAGT 480
D LSISSLKDLKILQRTPIRVLHRRSPL+REKIIHWMKIEKVAESSQYYLLHLCTQAGT
Sbjct: 421 ADLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHLCTQAGT 480
Query: 481 YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 524
YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHL E
Sbjct: 481 YIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE 521
BLAST of Pay0015555 vs. NCBI nr
Match:
XP_038897427.1 (tRNA pseudouridine synthase Pus10 [Benincasa hispida])
HSP 1 Score: 948.3 bits (2450), Expect = 2.7e-272
Identity = 476/531 (89.64%), Postives = 493/531 (92.84%), Query Frame = 0
Query: 1 MANEQDEVQANPEATPLALTSVTQEAISPGDK--------LQFPSYAAKDLLSIGVCIPC 60
MANEQD VQA+PEATP LTS TQEAISP D+ Q PS+A DLLSIGVCIPC
Sbjct: 1 MANEQDVVQADPEATP--LTSATQEAISPNDEPQLLNHALPQMPSHATIDLLSIGVCIPC 60
Query: 61 IIRLSGIERHNYSFSLSAPILGSDKEAIEDDNDRNTCCLCLGILQFSYVDDKGDFIKCKG 120
IIRLSGIERHNYSFSLS PILGSDKEAI+ DNDR+TC LCLG+LQFSY+DDKG++IK KG
Sbjct: 61 IIRLSGIERHNYSFSLSVPILGSDKEAIDADNDRSTCSLCLGVLQFSYIDDKGNYIKFKG 120
Query: 121 VDDLAVSISEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPI 180
DDLAVSISEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPI
Sbjct: 121 ADDLAVSISEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPI 180
Query: 181 CLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKI 240
CLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKAS NGNSIKRNQ+G KMRKI
Sbjct: 181 CLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASKSNGNSIKRNQNGCKMRKI 240
Query: 241 IGSDEPSDISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYS 300
G+DEPSDISNC+ NSVADDC NPSQEV FPQETIKEPCHLAL CYRTSIY+ GRYIKYS
Sbjct: 241 TGNDEPSDISNCDANSVADDCFNPSQEVEFPQETIKEPCHLALICYRTSIYLVGRYIKYS 300
Query: 301 RNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLV 360
RNVSQTRWVIGEERMGEASVEEI+GNNILP CRGD+YKFHAAGREDLDVRMLGSGRPFLV
Sbjct: 301 RNVSQTRWVIGEERMGEASVEEIIGNNILPLCRGDNYKFHAAGREDLDVRMLGSGRPFLV 360
Query: 361 EIQNARLLPSEMIINEIKSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALV 420
EIQNARLLPSEMIINEI+SKINS E KLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALV
Sbjct: 361 EIQNARLLPSEMIINEIQSKINSSENKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALV 420
Query: 421 WTSRPLEDGDFLSISSLKDLKILQRTPIRVLHRRSPLKREKIIHWMKIEKVAESSQYYLL 480
WTSRPLED D LSISSLKDLKILQRTPIRVLHRRSPL+REKIIHWMKIEKVAESSQYYLL
Sbjct: 421 WTSRPLEDDDLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLL 480
Query: 481 HLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 524
HLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMD H AE
Sbjct: 481 HLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDYHFAE 529
BLAST of Pay0015555 vs. TAIR 10
Match:
AT1G20410.1 (Pseudouridine synthase family protein )
HSP 1 Score: 505.4 bits (1300), Expect = 5.6e-143
Identity = 268/486 (55.14%), Postives = 335/486 (68.93%), Query Frame = 0
Query: 39 AAKDLLSIGVCIPCIIRLSGIERHNYSF-SLSAPIL------GSDKEAIEDDNDRNTCCL 98
A KDLLS+GVC CI RL +E + S+S L G D+ + + + C +
Sbjct: 41 AVKDLLSLGVCERCIFRLVSVEAFDSDISSVSTSTLRSWLKSGDDETSASESSCSTICIV 100
Query: 99 CLGILQFSYVDDKGDFIKCKGVDDLAVSISEMVKKEGHQIDSFSLEVSVPSIILENEKRV 158
CLGILQF + D K + +K D I+++VK++ H+ DSF LEVSVPS I+ENE+ +
Sbjct: 101 CLGILQFVFSDVKKELVKSDSSSDYVARITDLVKQDRHEFDSFGLEVSVPSTIMENERAL 160
Query: 159 WLYLKRKYGSEQWFQGKPPPICLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPK 218
YLK KY +E W Q +S KDALK+ + L+ L +S SS IRLTY+ K
Sbjct: 161 LSYLKGKYSTEVWLQRDK----ISVKDALKVLLLDQLKASLGAESDSSSFHIRLTYT--K 220
Query: 219 ASNGNGNSIKRNQDGFKMRKIIGSDEPSDISNCNVNSVADDCINPSQEVIFPQETIKEPC 278
AS+ + + + K RK +D N +CI+ + E + EPC
Sbjct: 221 ASDEAQGASETTHES-KRRK-------TDAEN------GSNCISENS-----FEKVYEPC 280
Query: 279 HLALTCYRTSIYMFGRYIKYSRNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKF 338
++ C R I+ GRY KYSRNVSQ+RW+I +ERMGEASVEEILG NILP C GDSYKF
Sbjct: 281 IFSVHCNRMPIFFSGRYFKYSRNVSQSRWIIDDERMGEASVEEILGGNILPACLGDSYKF 340
Query: 339 HAAGREDLDVRMLGSGRPFLVEIQNARLLPSEMIINEIKSKINSPETKLVGVRNLKVVGS 398
HAAGRED+DVRMLGSGRPFL+E+QN+R LPSE + E++ KIN E KLVGV++LK +GS
Sbjct: 341 HAAGREDIDVRMLGSGRPFLIEVQNSRQLPSEQSLKEVEEKINKSEKKLVGVKDLKCIGS 400
Query: 399 EGWALVQEGEAEKQKQYAALVWTSRPLEDGDFLSISSLKDLKILQRTPIRVLHRRSPLKR 458
+ WA+++EGEAEKQKQY ALVW SRPLE+ DF SIS LK+LKILQ+TP+RVLHRRSPL R
Sbjct: 401 QCWAMMREGEAEKQKQYVALVWISRPLEEKDFNSISCLKELKILQKTPVRVLHRRSPLDR 460
Query: 459 EKIIHWMKIEKVAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLD 518
EKIIHWMK+EK+ S Y+LLHLCTQAGTYIKEFVHGDLGRT PS+GSIL CRAEI+QLD
Sbjct: 461 EKIIHWMKVEKIKGHSHYFLLHLCTQAGTYIKEFVHGDLGRTTPSMGSILGCRAEIIQLD 501
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q3MIT2 | 3.9e-64 | 36.36 | tRNA pseudouridine synthase Pus10 OS=Homo sapiens OX=9606 GN=PUS10 PE=1 SV=1 | [more] |
Q9D3U0 | 4.7e-62 | 36.20 | tRNA pseudouridine synthase Pus10 OS=Mus musculus OX=10090 GN=Pus10 PE=1 SV=1 | [more] |
Q6ING2 | 5.8e-60 | 34.71 | tRNA pseudouridine synthase Pus10 OS=Xenopus laevis OX=8355 GN=pus10 PE=2 SV=1 | [more] |
A1RX33 | 4.9e-35 | 35.92 | tRNA pseudouridine synthase Pus10 OS=Thermofilum pendens (strain DSM 2475 / Hrk ... | [more] |
Q9VPK7 | 1.1e-34 | 29.72 | Putative tRNA pseudouridine synthase Pus10 OS=Drosophila melanogaster OX=7227 GN... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3BUF4 | 4.6e-302 | 98.85 | tRNA pseudouridine(55) synthase OS=Cucumis melo OX=3656 GN=LOC103493791 PE=3 SV=... | [more] |
A0A5D3D906 | 4.7e-299 | 96.09 | tRNA pseudouridine(55) synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... | [more] |
A0A5A7V921 | 2.6e-297 | 93.49 | tRNA pseudouridine(55) synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |
A0A0A0L378 | 3.6e-291 | 95.60 | tRNA pseudouridine(55) synthase OS=Cucumis sativus OX=3659 GN=Csa_4G653400 PE=3 ... | [more] |
E5GCD5 | 1.8e-269 | 89.39 | tRNA pseudouridine(55) synthase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
XP_008452922.1 | 9.5e-302 | 98.85 | PREDICTED: putative tRNA pseudouridine synthase Pus10 [Cucumis melo] | [more] |
TYK19969.1 | 9.8e-299 | 96.09 | putative tRNA pseudouridine synthase Pus10 [Cucumis melo var. makuwa] | [more] |
KAA0064623.1 | 5.4e-297 | 93.49 | putative tRNA pseudouridine synthase Pus10 [Cucumis melo var. makuwa] | [more] |
XP_011654255.1 | 7.5e-291 | 95.60 | putative tRNA pseudouridine synthase Pus10 [Cucumis sativus] >KGN55479.1 hypothe... | [more] |
XP_038897427.1 | 2.7e-272 | 89.64 | tRNA pseudouridine synthase Pus10 [Benincasa hispida] | [more] |
Match Name | E-value | Identity | Description | |
AT1G20410.1 | 5.6e-143 | 55.14 | Pseudouridine synthase family protein | [more] |