Pay0015296 (gene) Melon (Payzawat) v1

Overview
NamePay0015296
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Descriptionheat shock 70 kDa protein 8
Locationchr06: 3878378 .. 3881434 (-)
RNA-Seq ExpressionPay0015296
SyntenyPay0015296
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGATCAAGCTTTCTTTCAATGGCTTCTATAAATTACTCTTCGCTTTCTCCTTCTTTCTTCACCGACTTATCTCGTATCTTCTTAAGGTTTCTCGTTGATCTTTTCTTTTTAACTCTCTGCTACTGAATTTTGTCTGTCTCGTTGCTTGTTTCTGCCACCCGCTTGCTCGGCTCAACCATTTTAGTCGCTATTGTTCCTGGTAATTCCTCTTTTTCTTTCTTCAATTGGTTGGTTACGGAACTGGGTATGTCTTGTTCTTTGATTTTCTTGGCAATAGTTAGCAATTTTCAATCTGTTTGTGCTTTTCTAGACTGGGTTTGATGTATATTGCTTTTTTTTTCCTTCGTCTTTTTAAGTGACTGAGTCCTTGCTGCTTTGTCGAATTTTCTTAATGAAAATTCTTTTTCTGGTTTTCTACTGTTTACGTAGAGAAATGATGTTTGGAATTGCCCGGAAGGTGTTTGTTAATGTAGTGCTTATTAATTTCAACGTGTATGCTGATGATTGAAATGTCATAATTGTAACGAGAATTGGATTAGAGAGATAGATTACAATTGATCTTGTTTATTTGAGTTTATGCAATTTCTGTCCTAATGAATCGGCCTATGTGAAATAAAATTTGATTTAACGGGCGTATGTTCATATTTAGAATTCAGTGTTAAACAGTATTTTCTATGATTTGTAAGAAAAAGACCCTATATGATATGGTCTGTGTTTGTAACACCTTCCGAAGTGATAGAGGCGATCTCATTTTGTCCAAAGTGTGTTTCTCCTCTTTCTCTGTCATAACTAGAACGAGCATATTCCTTTTGAAGTGTTCGAAAGATGATTGAGTGGACTTTAAATCCCATTTTAAGGGGTAGAATTGTTGATTATCTTTTTCATGTCCTCTAGCGCAATGTATGATTAGTAATGACAGTGAGTTACCCACTCAAAACTAGACCTATATATTGATCAAAATGCAGATTATGGCTGAACCAATATACACAGTAGCTTCTGATAGTGAAACTACTGGGGAAGATAGAACAACATCAACTTTTCCAGAAATTGCAGTTGGAATTGATATTGGCACTTCACAGTGTAGTATTGCAGTCTGGAATGGATCTCTGGTAGAGCTTCTTAAGAACACAAGAGATCAGAAAATTATGAGATCATATGTCACCTTCAAGGATGACACTCCTTCTGGGGGAGTTAGCAATGAGCTAAGCCATGAGTATGACATGCTCTCTGGTGCTGCAATTTTCAATATGAAACGCTTGATTGGAAGAGTTGACACTGATCCAGTTGTTCATGCATGCAAGAGCCTTCCTTTTCTAGTGCAAACTTTGGACATTGGTGTTCGTCCATTTGTTGCAGCTTTGGTGAATAATGTATGGAGATCTACAACTCCTGAAGAAGTTTTGGCAATCTTCATGGTTGAACTGAAAATGATGGCTGAATATCAACTGAAACGACCAATTAGAAATGTTGTTTTGACAATTCCAGTTTCATTCAGCCGATTCCAGCTTACTCGTGTGGAACGAGCTTGTGCAATGGCTGGATTTCAAGTTAGATTAATGCCTGAACCAACAGCAGTAGCTTTATTATATGCTCAACAACAACAGCAGGCTGCGCATGACAGCATGGGCAGTGGAAGTGAAAAAATTGCTCTTATTTTCAACATGGGAGCTGGATATTGTGATGCTGCTGTGTCAGCCATAGGAGGAGGGGTGTCACAGATCAGAGCCCTGGCTGGCAGTCCTATAGGAGGCGAAGATCTACTTCAAAACACCATGAAGTATCTTTTGCCAAATTCGGAATCTATATTCTCACATCGTGGAATTGATGAGATCAGAAGGATGGGCTTGCTCCGAGTTGCAACTCAAGATGCTATCCACAAGCTCTCGTTCCAGTCTAGTGTTGAAATCAATGTCAGCTTGGGAAATGGGTCGAAGTTATGCAAGGTTCTAAGCCGAGAGGAGTTTGAGATGGTGAACAGCAAGGTGTTCGAGAAATGTGAGAACCTTGTTAAGCAATGTTTACATGATGCACGAGTCGGGATCGAAGATTTGAGTGATGTAGTAGTGGTTGGCGGATGTTCATACATACCAAAGATCAGAAAGCTTCTGATGGAAATAAGTAAAAAGAAGGCACTTTATGAAGGAATAGATCCTTTAGAAGCTGCAGTTTCTGGTGCAGCTTTAGAAGGTGCAATAGCATCAGGCATTGGCGATCCATTTGGCAGTCTAGATCTTCTTAGCATCCAAGCAACGCCTCTTGCCATTGGAATTCGAGCTGATGGAAACAGTTTCATTCCAATTATCCCCAAGAACACCACAATGCCAGCACGCAAGGAGTTAGTTTTCACAACAGTTCAAGACAATCAAAGTGAGGCCCTAATTGTTGTCTATGAAGGTGAAGAGACAAGAGCAGAGGAAAACCACCTGCTTGGCTATTTCAAAATCATTGGAATTCCACCAGCACCCAAAGGAGTCCCTGAAATCACTATTTGTATGGACATTGACTCTTCAAATATGCTCAGAGTTCTAGCTGGGGCAGCACTCCCAGGTGCTCAACACCCTGCAACTCCTTACATGGAAGTCAAGATGCCGACCGTGGATGATGGACATGGCTGGTGTGCAGAAGCTCTGCATGGAAAATATGGTGCGACCATGGAATTGGTTACATTGCGGAAGAAAAGCTAGGTGATTTATCTAGGCCAAGCGGCCGTTCTGTTTCTACATTACATTTCCATATAACTCATAAGTTCAATACAGGTACATAATGAAATAATGTATGGTGGGTTATGTAACTCTTACACTGCATGTTTTGAATTGTCAATAGCATCCTAGAATCTAAACTAAGTAGGAACTCTTGTAAGTTGGCGATGGTGTAATTATGCAGAGATCCTTTAAGTTGTAAGTTGTCACAAGCTTGACAAGTGTATCCATAGGTTGTGGCATGTTCTCTTTGTTCATAATATTAAAGGCTTGACATCTGACAATGACTTGACCTTTGATGCATCAAGGACACAAGGAGACAATTTGTTGTCTCAAATCACTCACCAAAATGATTTTC

mRNA sequence

CGATCAAGCTTTCTTTCAATGGCTTCTATAAATTACTCTTCGCTTTCTCCTTCTTTCTTCACCGACTTATCTCGTATCTTCTTAAGGTTTCTCGTTGATCTTTTCTTTTTAACTCTCTGCTACTGAATTTTGTCTGTCTCGTTGCTTGTTTCTGCCACCCGCTTGCTCGGCTCAACCATTTTAGTCGCTATTGTTCCTGATTATGGCTGAACCAATATACACAGTAGCTTCTGATAGTGAAACTACTGGGGAAGATAGAACAACATCAACTTTTCCAGAAATTGCAGTTGGAATTGATATTGGCACTTCACAGTGTAGTATTGCAGTCTGGAATGGATCTCTGGTAGAGCTTCTTAAGAACACAAGAGATCAGAAAATTATGAGATCATATGTCACCTTCAAGGATGACACTCCTTCTGGGGGAGTTAGCAATGAGCTAAGCCATGAGTATGACATGCTCTCTGGTGCTGCAATTTTCAATATGAAACGCTTGATTGGAAGAGTTGACACTGATCCAGTTGTTCATGCATGCAAGAGCCTTCCTTTTCTAGTGCAAACTTTGGACATTGGTGTTCGTCCATTTGTTGCAGCTTTGGTGAATAATGTATGGAGATCTACAACTCCTGAAGAAGTTTTGGCAATCTTCATGGTTGAACTGAAAATGATGGCTGAATATCAACTGAAACGACCAATTAGAAATGTTGTTTTGACAATTCCAGTTTCATTCAGCCGATTCCAGCTTACTCGTGTGGAACGAGCTTGTGCAATGGCTGGATTTCAAGTTAGATTAATGCCTGAACCAACAGCAGTAGCTTTATTATATGCTCAACAACAACAGCAGGCTGCGCATGACAGCATGGGCAGTGGAAGTGAAAAAATTGCTCTTATTTTCAACATGGGAGCTGGATATTGTGATGCTGCTGTGTCAGCCATAGGAGGAGGGGTGTCACAGATCAGAGCCCTGGCTGGCAGTCCTATAGGAGGCGAAGATCTACTTCAAAACACCATGAAGTATCTTTTGCCAAATTCGGAATCTATATTCTCACATCGTGGAATTGATGAGATCAGAAGGATGGGCTTGCTCCGAGTTGCAACTCAAGATGCTATCCACAAGCTCTCGTTCCAGTCTAGTGTTGAAATCAATGTCAGCTTGGGAAATGGGTCGAAGTTATGCAAGGTTCTAAGCCGAGAGGAGTTTGAGATGGTGAACAGCAAGGTGTTCGAGAAATGTGAGAACCTTGTTAAGCAATGTTTACATGATGCACGAGTCGGGATCGAAGATTTGAGTGATGTAGTAGTGGTTGGCGGATGTTCATACATACCAAAGATCAGAAAGCTTCTGATGGAAATAAGTAAAAAGAAGGCACTTTATGAAGGAATAGATCCTTTAGAAGCTGCAGTTTCTGGTGCAGCTTTAGAAGGTGCAATAGCATCAGGCATTGGCGATCCATTTGGCAGTCTAGATCTTCTTAGCATCCAAGCAACGCCTCTTGCCATTGGAATTCGAGCTGATGGAAACAGTTTCATTCCAATTATCCCCAAGAACACCACAATGCCAGCACGCAAGGAGTTAGTTTTCACAACAGTTCAAGACAATCAAAGTGAGGCCCTAATTGTTGTCTATGAAGGTGAAGAGACAAGAGCAGAGGAAAACCACCTGCTTGGCTATTTCAAAATCATTGGAATTCCACCAGCACCCAAAGGAGTCCCTGAAATCACTATTTGTATGGACATTGACTCTTCAAATATGCTCAGAGTTCTAGCTGGGGCAGCACTCCCAGGTGCTCAACACCCTGCAACTCCTTACATGGAAGTCAAGATGCCGACCGTGGATGATGGACATGGCTGGTGTGCAGAAGCTCTGCATGGAAAATATGGTGCGACCATGGAATTGGTTACATTGCGGAAGAAAAGCTAGGTGATTTATCTAGGCCAAGCGGCCGTTCTGTTTCTACATTACATTTCCATATAACTCATAAGTTCAATACAGGTACATAATGAAATAATGTATGGTGGGTTATGTAACTCTTACACTGCATGTTTTGAATTGTCAATAGCATCCTAGAATCTAAACTAAGTAGGAACTCTTGTAAGTTGGCGATGGTGTAATTATGCAGAGATCCTTTAAGTTGTAAGTTGTCACAAGCTTGACAAGTGTATCCATAGGTTGTGGCATGTTCTCTTTGTTCATAATATTAAAGGCTTGACATCTGACAATGACTTGACCTTTGATGCATCAAGGACACAAGGAGACAATTTGTTGTCTCAAATCACTCACCAAAATGATTTTC

Coding sequence (CDS)

ATGGCTGAACCAATATACACAGTAGCTTCTGATAGTGAAACTACTGGGGAAGATAGAACAACATCAACTTTTCCAGAAATTGCAGTTGGAATTGATATTGGCACTTCACAGTGTAGTATTGCAGTCTGGAATGGATCTCTGGTAGAGCTTCTTAAGAACACAAGAGATCAGAAAATTATGAGATCATATGTCACCTTCAAGGATGACACTCCTTCTGGGGGAGTTAGCAATGAGCTAAGCCATGAGTATGACATGCTCTCTGGTGCTGCAATTTTCAATATGAAACGCTTGATTGGAAGAGTTGACACTGATCCAGTTGTTCATGCATGCAAGAGCCTTCCTTTTCTAGTGCAAACTTTGGACATTGGTGTTCGTCCATTTGTTGCAGCTTTGGTGAATAATGTATGGAGATCTACAACTCCTGAAGAAGTTTTGGCAATCTTCATGGTTGAACTGAAAATGATGGCTGAATATCAACTGAAACGACCAATTAGAAATGTTGTTTTGACAATTCCAGTTTCATTCAGCCGATTCCAGCTTACTCGTGTGGAACGAGCTTGTGCAATGGCTGGATTTCAAGTTAGATTAATGCCTGAACCAACAGCAGTAGCTTTATTATATGCTCAACAACAACAGCAGGCTGCGCATGACAGCATGGGCAGTGGAAGTGAAAAAATTGCTCTTATTTTCAACATGGGAGCTGGATATTGTGATGCTGCTGTGTCAGCCATAGGAGGAGGGGTGTCACAGATCAGAGCCCTGGCTGGCAGTCCTATAGGAGGCGAAGATCTACTTCAAAACACCATGAAGTATCTTTTGCCAAATTCGGAATCTATATTCTCACATCGTGGAATTGATGAGATCAGAAGGATGGGCTTGCTCCGAGTTGCAACTCAAGATGCTATCCACAAGCTCTCGTTCCAGTCTAGTGTTGAAATCAATGTCAGCTTGGGAAATGGGTCGAAGTTATGCAAGGTTCTAAGCCGAGAGGAGTTTGAGATGGTGAACAGCAAGGTGTTCGAGAAATGTGAGAACCTTGTTAAGCAATGTTTACATGATGCACGAGTCGGGATCGAAGATTTGAGTGATGTAGTAGTGGTTGGCGGATGTTCATACATACCAAAGATCAGAAAGCTTCTGATGGAAATAAGTAAAAAGAAGGCACTTTATGAAGGAATAGATCCTTTAGAAGCTGCAGTTTCTGGTGCAGCTTTAGAAGGTGCAATAGCATCAGGCATTGGCGATCCATTTGGCAGTCTAGATCTTCTTAGCATCCAAGCAACGCCTCTTGCCATTGGAATTCGAGCTGATGGAAACAGTTTCATTCCAATTATCCCCAAGAACACCACAATGCCAGCACGCAAGGAGTTAGTTTTCACAACAGTTCAAGACAATCAAAGTGAGGCCCTAATTGTTGTCTATGAAGGTGAAGAGACAAGAGCAGAGGAAAACCACCTGCTTGGCTATTTCAAAATCATTGGAATTCCACCAGCACCCAAAGGAGTCCCTGAAATCACTATTTGTATGGACATTGACTCTTCAAATATGCTCAGAGTTCTAGCTGGGGCAGCACTCCCAGGTGCTCAACACCCTGCAACTCCTTACATGGAAGTCAAGATGCCGACCGTGGATGATGGACATGGCTGGTGTGCAGAAGCTCTGCATGGAAAATATGGTGCGACCATGGAATTGGTTACATTGCGGAAGAAAAGCTAG

Protein sequence

MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIMRSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAAVSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKMPTVDDGHGWCAEALHGKYGATMELVTLRKKS
Homology
BLAST of Pay0015296 vs. ExPASy Swiss-Prot
Match: Q9SKY8 (Heat shock 70 kDa protein 8 OS=Arabidopsis thaliana OX=3702 GN=HSP70-8 PE=1 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 3.4e-239
Identity = 403/573 (70.33%), Postives = 488/573 (85.17%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEDRTTS--TFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQK 60
           MAE  YTVASDSE TGE++++S  + PEIA+GIDIGTSQCSIAVWNGS V +L+NTR+QK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 61  IMRSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQ 120
           +++S+VTFKD+ P+GGVSN+L+HE +ML+GAAIFNMKRL+GRVDTDPVVHA K+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 121 TLDIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRF 180
           TLDIGVRPF+AALVNN WRSTTPEEVLAIF+VEL++MAE QLKRP+RNVVLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 181 QLTRVERACAMAGFQV-RLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYC 240
           QLTR ERACAMAG  V RLMPEPTA+ALLYAQQQQ   HD+MGSGSE++A+IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 241 DAAVSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVA 300
           D AV+A  GGVSQI+ALAGSPIGGED+LQNT++++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 301 TQDAIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVG 360
            QDAIH+L+ Q +V+I V LGNG+K+ KVL R EFE VN KVFE+CE LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 361 IEDLSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPF 420
             D+ D+++VGGCSYIPK+R ++  + KK  +Y+G++PLEAAV GAALEGA+ SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 421 GSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGE 480
           GSLDLL+IQATPLA+G+RA+GN FIP+IP+NT +PARK+L FTTVQDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 481 ETRAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYME 540
               EENHLLGYFK++GIPPAPKGVPEI +CMDID+SN LRV A   +PG+  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 541 VKMPTVDDGHGWCAEALHGKYGATMELVTLRKK 571
           V+MPTVDDGHGWCA+AL+ KYGAT++L+TL++K
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRK 562

BLAST of Pay0015296 vs. ExPASy Swiss-Prot
Match: Q9LHA8 (Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 1.1e-75
Identity = 179/511 (35.03%), Postives = 285/511 (55.77%), Query Frame = 0

Query: 28  AVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIMRSYVTFKDDTPSGGVSNELSHEYDMLS 87
           A+GID+GT+   + VW    VE++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 88  GAAIFNMKRLIGRVDTDPVVHACKS-LPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLA 147
              +F+ KRLIGR  +DP V A KS  PF V +   G +P +        +  + EE+ +
Sbjct: 69  TNTVFDAKRLIGRRYSDPSVQADKSHWPFKVVS-GPGEKPMIVVNHKGEEKQFSAEEISS 128

Query: 148 IFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGFQV-RLMPEPTAVAL 207
           + +++++ +AE  L  P++N V+T+P  F+  Q    + A  ++G  V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAI 188

Query: 208 LYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAAVSAIGGGVSQIRALAG-SPIGGEDL 267
            Y   ++ +      S  EK  LIF++G G  D ++  I  G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAS------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 268 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSSVEINVSLGNGSK 327
               + + +   +           R +  LR A + A   LS   Q+++EI+ SL  G  
Sbjct: 249 DNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEID-SLFEGID 308

Query: 328 LCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKIRKLLME 387
               ++R  FE +N  +F KC   V++CL DA++    + DVV+VGG + IPK+++LL +
Sbjct: 309 FYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQD 368

Query: 388 ISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRADGNSFI 447
               K L + I+P EA   GAA++ AI SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQAAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 448 PIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPKGV 507
            +IP+NTT+P +KE +F+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPAP+GV
Sbjct: 429 VLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 508 PEITICMDIDSSNMLRVLAGAALPGAQHPAT 534
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Pay0015296 vs. ExPASy Swiss-Prot
Match: Q9S9N1 (Heat shock 70 kDa protein 5 OS=Arabidopsis thaliana OX=3702 GN=HSP70-5 PE=2 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 9.1e-75
Identity = 181/517 (35.01%), Postives = 282/517 (54.55%), Query Frame = 0

Query: 22  STFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIMRSYVTFKDDTPSGGVSNELSH 81
           +T  E A+GID+GT+   + VW    VE++ N +  +   SYV F D      + +   +
Sbjct: 2   ATKSEKAIGIDLGTTYSCVGVWMNDRVEIIPNDQGNRTTPSYVAFTDTERL--IGDAAKN 61

Query: 82  EYDMLSGAAIFNMKRLIGRVDTDPVVHA-CKSLPFLVQTLDIGVRPFVAALVNNVWRSTT 141
           +  +     +F+ KRLIGR  +DP V +     PF V +   G +P +     N  +  +
Sbjct: 62  QVALNPQNTVFDAKRLIGRKFSDPSVQSDILHWPFKVVS-GPGEKPMIVVSYKNEEKQFS 121

Query: 142 PEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGFQV-RLMPE 201
           PEE+ ++ +V++K +AE  L R ++N V+T+P  F+  Q    + A A++G  V R++ E
Sbjct: 122 PEEISSMVLVKMKEVAEAFLGRTVKNAVVTVPAYFNDSQRQATKDAGAISGLNVLRIINE 181

Query: 202 PTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAAVSAIGGGVSQIRALAG-SP 261
           PTA A+ Y   ++           EK  LIF++G G  D ++  I  GV +++A AG + 
Sbjct: 182 PTAAAIAYGLDKKGT------KAGEKNVLIFDLGGGTFDVSLLTIEEGVFEVKATAGDTH 241

Query: 262 IGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSSVEINVS 321
           +GGED     + + +               R +  LR A + A   LS   Q+++EI+ S
Sbjct: 242 LGGEDFDNRLVNHFVAEFRRKHKKDIAGNARALRRLRTACERAKRTLSSTAQTTIEID-S 301

Query: 322 LGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKI 381
           L  G      +SR  FE +N  +F KC + V++ L DA++    + DVV+VGG + IPKI
Sbjct: 302 LHEGIDFYATISRARFEEMNMDLFRKCMDPVEKVLKDAKLDKSSVHDVVLVGGSTRIPKI 361

Query: 382 RKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRA 441
           ++LL +    K L + I+P EA   GAA++ AI +G G      DLL +   PL++G+  
Sbjct: 362 QQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGSE-KVQDLLLLDVAPLSLGLET 421

Query: 442 DGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIP 501
            G     +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  R  +N+LLG F++ GIP
Sbjct: 422 AGGVMTVLIPRNTTVPCKKEQVFSTYADNQPGVLIQVYEGERARTRDNNLLGTFELKGIP 481

Query: 502 PAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPAT 534
           PAP+GVP+I +C DID++ +L V A     G ++  T
Sbjct: 482 PAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQIT 507

BLAST of Pay0015296 vs. ExPASy Swiss-Prot
Match: P09189 (Heat shock cognate 70 kDa protein OS=Petunia hybrida OX=4102 GN=HSP70 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.2e-74
Identity = 182/515 (35.34%), Postives = 283/515 (54.95%), Query Frame = 0

Query: 28  AVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIMRSYVTFKDDTPSGGVSNELSHEYDMLS 87
           A+GID+GT+   + VW    VE++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERL--IGDAAKNQVAMNP 68

Query: 88  GAAIFNMKRLIGRVDTDPVVHA-CKSLPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLA 147
              +F+ KRLIGR  +DP V +  K  PF V     G +P +        +    EE+ +
Sbjct: 69  INTVFDAKRLIGRRFSDPSVQSDIKLWPFKV-IPGPGDKPMIVVTYKGEEKQFAAEEISS 128

Query: 148 IFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGFQV-RLMPEPTAVAL 207
           + + ++K +AE  L   I+N V+T+P  F+  Q    + A  +AG  V R++ EPTA A+
Sbjct: 129 MVLTKMKEIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAI 188

Query: 208 LYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAAVSAIGGGVSQIRALAG-SPIGGEDL 267
            Y   ++ +      S  EK  LIF++G G  D ++  I  G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAS------SAGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 268 LQNTMKYLLPNSESIFSHRGIDEI----RRMGLLRVATQDAIHKLS--FQSSVEINVSLG 327
               + + +      F  +   +I    R +  LR A + A   LS   Q+++EI+ SL 
Sbjct: 249 DNRMVNHFVQE----FKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEID-SLY 308

Query: 328 NGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKIRK 387
            G      ++R  FE +N  +F KC   V++CL DA++    + DVV+VGG + IPK+++
Sbjct: 309 EGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQ 368

Query: 388 LLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRADG 447
           LL +    K L + I+P EA   GAA++ AI SG G+     DLL +  TPL++G+   G
Sbjct: 369 LLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNE-KVQDLLLLDVTPLSLGLETAG 428

Query: 448 NSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPA 507
                +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPA
Sbjct: 429 GGMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPA 488

Query: 508 PKGVPEITICMDIDSSNMLRVLAGAALPGAQHPAT 534
           P+GVP+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Pay0015296 vs. ExPASy Swiss-Prot
Match: P22953 (Heat shock 70 kDa protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP70-1 PE=1 SV=3)

HSP 1 Score: 278.9 bits (712), Expect = 1.3e-73
Identity = 177/511 (34.64%), Postives = 280/511 (54.79%), Query Frame = 0

Query: 28  AVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIMRSYVTFKDDTPSGGVSNELSHEYDMLS 87
           A+GID+GT+   + VW    VE++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 88  GAAIFNMKRLIGRVDTDPVVHA-CKSLPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLA 147
              +F+ KRLIGR  +D  V +  K  PF +Q      +P +        +    EE+ +
Sbjct: 69  VNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQA-GPADKPMIYVEYKGEEKEFAAEEISS 128

Query: 148 IFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGFQV-RLMPEPTAVAL 207
           + +++++ +AE  L   I+N V+T+P  F+  Q    + A  +AG  V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAI 188

Query: 208 LYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAAVSAIGGGVSQIRALAG-SPIGGEDL 267
            Y   ++        S  EK  LIF++G G  D ++  I  G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAT------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 268 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSSVEINVSLGNGSK 327
               + + +   +           R +  LR + + A   LS   Q+++EI+ SL  G  
Sbjct: 249 DNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEID-SLYEGID 308

Query: 328 LCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKIRKLLME 387
               ++R  FE +N  +F KC   V++CL DA++    + DVV+VGG + IPK+++LL +
Sbjct: 309 FYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQD 368

Query: 388 ISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRADGNSFI 447
               K L + I+P EA   GAA++GAI SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQGAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 448 PIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPKGV 507
            +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPAP+GV
Sbjct: 429 TLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 508 PEITICMDIDSSNMLRVLAGAALPGAQHPAT 534
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Pay0015296 vs. ExPASy TrEMBL
Match: A0A1S3AXP0 (heat shock 70 kDa protein 8 OS=Cucumis melo OX=3656 GN=LOC103483715 PE=3 SV=1)

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 571/571 (100.00%), Postives = 571/571 (100.00%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60
           MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240
           TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240

Query: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360
           AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420
           LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 572
           PTVDDGHGWCAEALHGKYGATMELVTLRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Pay0015296 vs. ExPASy TrEMBL
Match: A0A5D3D052 (Heat shock 70 kDa protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00370 PE=3 SV=1)

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 570/571 (99.82%), Postives = 570/571 (99.82%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60
           MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240
           TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCD A
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360
           AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420
           LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 572
           PTVDDGHGWCAEALHGKYGATMELVTLRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Pay0015296 vs. ExPASy TrEMBL
Match: A0A0A0LAC6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G147740 PE=3 SV=1)

HSP 1 Score: 1102.4 bits (2850), Expect = 0.0e+00
Identity = 560/571 (98.07%), Postives = 565/571 (98.95%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60
           MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240
           TRVERACAMAG QVRLMPEPTAVALLYAQQQQQA HDSMGSGSEKIALIFNMGAGYCD A
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360
           AIHKLSFQSSVEINVSLGNGSKLCKVLSRE+FEMVNSKVFEKCENLVKQCLHDAR+GIED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARLGIED 360

Query: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420
           LSDVVVVGGCSYIPKIR LLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTT+QDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGA LPGAQHPATPYMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 572
           PTVDDGHGWCAEALHGKYGATMELVTLRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Pay0015296 vs. ExPASy TrEMBL
Match: A0A6J1GXD4 (heat shock 70 kDa protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111457701 PE=3 SV=1)

HSP 1 Score: 1041.2 bits (2691), Expect = 1.6e-300
Identity = 529/572 (92.48%), Postives = 551/572 (96.33%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60
           MAEPIYTVASDSETTGEDR+ S FPEIAVGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRSASIFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120
           RSYVTFKDDTPSGGV NELS EYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVINELSQEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           DIGVRPFVAALVNN+WRSTTPEEVLAIF+VELKMMAE+QLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNIWRSTTPEEVLAIFLVELKMMAEHQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGFQVRLMPEPTAVALLYA-QQQQQAAHDSMGSGSEKIALIFNMGAGYCDA 240
           TRVERACAMAG QVRLMPEPTAVALLYA QQQQQA H++MGSGSEKIALIFNMGAGYCD 
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQQAVHENMGSGSEKIALIFNMGAGYCDV 240

Query: 241 AVSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQ 300
           AVSA GGGVSQI+ALAGSPIGGEDLLQNTMKYLLPN+ESIFSHRGIDEIRRMGLLRVATQ
Sbjct: 241 AVSATGGGVSQIKALAGSPIGGEDLLQNTMKYLLPNAESIFSHRGIDEIRRMGLLRVATQ 300

Query: 301 DAIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIE 360
           DAIHKLSFQSSV+I+V+LGNGSKLCKVLSREEFEMVNS+VFEKCENLV QCLHDARVGI+
Sbjct: 301 DAIHKLSFQSSVQIDVNLGNGSKLCKVLSREEFEMVNSEVFEKCENLVNQCLHDARVGID 360

Query: 361 DLSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGS 420
           DLSDVVVVGGCS IPKIR  LME SKKKALYEG++PLEAAV GAALEGA+ASGI DPFGS
Sbjct: 361 DLSDVVVVGGCSNIPKIRSFLMEKSKKKALYEGMNPLEAAVCGAALEGAVASGIADPFGS 420

Query: 421 LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEET 480
           LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTV+DNQSEALIVVYEGEET
Sbjct: 421 LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVKDNQSEALIVVYEGEET 480

Query: 481 RAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVK 540
           RAEENHLLGYFKIIGIPPAPKG+PEITICMDIDSSNMLRVLAGA LPGAQHPATP+MEVK
Sbjct: 481 RAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGARLPGAQHPATPFMEVK 540

Query: 541 MPTVDDGHGWCAEALHGKYGATMELVTLRKKS 572
           MPTVDDG GWCAEALHGKYGA M+LV+LRKKS
Sbjct: 541 MPTVDDGQGWCAEALHGKYGAAMDLVSLRKKS 572

BLAST of Pay0015296 vs. ExPASy TrEMBL
Match: A0A6J1C992 (heat shock 70 kDa protein 8 OS=Momordica charantia OX=3673 GN=LOC111009482 PE=3 SV=1)

HSP 1 Score: 1040.4 bits (2689), Expect = 2.7e-300
Identity = 525/571 (91.94%), Postives = 549/571 (96.15%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120
           RSYVTFKDDTPSGGVS+EL HEYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSSELCHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           DIGVRPFVAALVNN+WRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNMWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240
           TRVERACAMAG QVRLMPEPTAVALLYAQQQ QA H++MGSGSEKIAL+FNMGAGYCD A
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQHQAVHENMGSGSEKIALVFNMGAGYCDVA 240

Query: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           +SA  GGVSQIRAL GSPIGGEDLLQNTMKYLLPNSESIFS+ GIDEIRRMGLLRVATQD
Sbjct: 241 ISATAGGVSQIRALTGSPIGGEDLLQNTMKYLLPNSESIFSNHGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360
           AI KLSFQ+S +I+V+LGNGSK+CKVLSREEFEMVNSKVFEKCENLVKQCLHDARV IED
Sbjct: 301 AILKLSFQTSAQIDVNLGNGSKICKVLSREEFEMVNSKVFEKCENLVKQCLHDARVEIED 360

Query: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420
           LSDVVVVGGCS IPKIRKLLMEISKKKALYE IDPLEAAVSGAA+EGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSSIPKIRKLLMEISKKKALYEEIDPLEAAVSGAAIEGAVASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQ TPLAIGIRADGN+FIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEE R
Sbjct: 421 DLLSIQVTPLAIGIRADGNNFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEEAR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSN LRVLAGA LPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNTLRVLAGATLPGAQHPATPFMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 572
           PT+DDGH WCAEALHGK+G +M+LVTLRKKS
Sbjct: 541 PTIDDGHSWCAEALHGKHGVSMDLVTLRKKS 571

BLAST of Pay0015296 vs. NCBI nr
Match: XP_008438682.1 (PREDICTED: heat shock 70 kDa protein 8 [Cucumis melo])

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 571/571 (100.00%), Postives = 571/571 (100.00%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60
           MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240
           TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240

Query: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360
           AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420
           LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 572
           PTVDDGHGWCAEALHGKYGATMELVTLRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Pay0015296 vs. NCBI nr
Match: KAA0049367.1 (heat shock 70 kDa protein 8 [Cucumis melo var. makuwa] >TYK17192.1 heat shock 70 kDa protein 8 [Cucumis melo var. makuwa])

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 570/571 (99.82%), Postives = 570/571 (99.82%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60
           MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240
           TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCD A
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360
           AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420
           LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 572
           PTVDDGHGWCAEALHGKYGATMELVTLRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Pay0015296 vs. NCBI nr
Match: XP_004134140.1 (heat shock 70 kDa protein 8 [Cucumis sativus] >XP_011650982.1 heat shock 70 kDa protein 8 [Cucumis sativus] >KGN56991.1 hypothetical protein Csa_011014 [Cucumis sativus])

HSP 1 Score: 1102.4 bits (2850), Expect = 0.0e+00
Identity = 560/571 (98.07%), Postives = 565/571 (98.95%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60
           MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240
           TRVERACAMAG QVRLMPEPTAVALLYAQQQQQA HDSMGSGSEKIALIFNMGAGYCD A
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360
           AIHKLSFQSSVEINVSLGNGSKLCKVLSRE+FEMVNSKVFEKCENLVKQCLHDAR+GIED
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARLGIED 360

Query: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420
           LSDVVVVGGCSYIPKIR LLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTT+QDNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGA LPGAQHPATPYMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 572
           PTVDDGHGWCAEALHGKYGATMELVTLRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Pay0015296 vs. NCBI nr
Match: XP_038897373.1 (heat shock 70 kDa protein 8 [Benincasa hispida])

HSP 1 Score: 1070.8 bits (2768), Expect = 3.9e-309
Identity = 544/571 (95.27%), Postives = 558/571 (97.72%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60
           MAEPIYTVASDSETTGED+TTSTFP+IAVGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEDKTTSTFPDIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120
           RSYVTFKDDTPSGGVSNELSHE+DMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEFDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKR I+NVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRSIKNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240
           TRVERACAMAG QVRLMPEPTAVALLYAQQQQQA H+SMGSGSEKIALIFNMGAGYCD A
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHESMGSGSEKIALIFNMGAGYCDVA 240

Query: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300
           VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360
           AIHKLSFQSSV+I+V+LGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARV IED
Sbjct: 301 AIHKLSFQSSVQIDVNLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVRIED 360

Query: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420
           LSDVVVVGGCSYIPKIR LLMEI K KALYEG+DPLEAAVSGAALEGAIASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRNLLMEICKNKALYEGMDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480
           DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTV+DNQSEALIVVYEGEETR
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVRDNQSEALIVVYEGEETR 480

Query: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540
           AEENHLLGY KIIGIPPAPKGVPEITICMDIDSSNMLRVLAGA LPG+QHPATPYMEVKM
Sbjct: 481 AEENHLLGYVKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPYMEVKM 540

Query: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 572
           PTVDDGHGWCAEALHGKYG TMELV+LRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGPTMELVSLRKKS 571

BLAST of Pay0015296 vs. NCBI nr
Match: XP_023527609.1 (heat shock 70 kDa protein 8 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1043.1 bits (2696), Expect = 8.7e-301
Identity = 532/572 (93.01%), Postives = 551/572 (96.33%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60
           MAEPIYTVASDSETTGEDR+ S FPEI VGIDIGTSQCSIAVWNGS VELLKNTR+QKIM
Sbjct: 1   MAEPIYTVASDSETTGEDRSASIFPEIDVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120
           RSYVTFKDDTPSGGV NELS EYDMLSGAAIFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVINELSQEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180
           DIGVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAE+QLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEHQLKRPIRNVVLTIPVSFSRFQL 180

Query: 181 TRVERACAMAGFQVRLMPEPTAVALLYA-QQQQQAAHDSMGSGSEKIALIFNMGAGYCDA 240
           TRVERACAMAG QVRLMPEPTAVALLYA QQQQQAAH++MGSGSEKIALIFNMGAGYCD 
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQQAAHENMGSGSEKIALIFNMGAGYCDV 240

Query: 241 AVSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQ 300
           AVSA GGGVSQI+ALAGSPIGGEDLLQNTMKYLLPN+ESIFSHRGIDEIRRMGLLRVATQ
Sbjct: 241 AVSATGGGVSQIKALAGSPIGGEDLLQNTMKYLLPNAESIFSHRGIDEIRRMGLLRVATQ 300

Query: 301 DAIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIE 360
           DAIHKLSFQSSV+I+V+LGNGSKLCKVLSREEFEMVNS+VFEKCENLV  CLHDARVGIE
Sbjct: 301 DAIHKLSFQSSVQIDVNLGNGSKLCKVLSREEFEMVNSEVFEKCENLVNLCLHDARVGIE 360

Query: 361 DLSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGS 420
           DLSDVVVVGGCS IPKIR LLM  SKKKALYEG++PLEAAVSGAALEGA+ASGI DPFGS
Sbjct: 361 DLSDVVVVGGCSNIPKIRNLLMGKSKKKALYEGMNPLEAAVSGAALEGAVASGIADPFGS 420

Query: 421 LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEET 480
           LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTV+DNQSEALIVVYEGEET
Sbjct: 421 LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVKDNQSEALIVVYEGEET 480

Query: 481 RAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVK 540
           RAEENHLLGYFKIIGIPPAPKG+PEITICMDIDSSNMLRVLAGA LPG QHPATP+MEVK
Sbjct: 481 RAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGARLPGVQHPATPFMEVK 540

Query: 541 MPTVDDGHGWCAEALHGKYGATMELVTLRKKS 572
           MPTVDDGHGWCAEALHGKYGA M+LVTLRKKS
Sbjct: 541 MPTVDDGHGWCAEALHGKYGAAMDLVTLRKKS 572

BLAST of Pay0015296 vs. TAIR 10
Match: AT2G32120.1 (heat-shock protein 70T-2 )

HSP 1 Score: 828.9 bits (2140), Expect = 2.4e-240
Identity = 403/573 (70.33%), Postives = 488/573 (85.17%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEDRTTS--TFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQK 60
           MAE  YTVASDSE TGE++++S  + PEIA+GIDIGTSQCSIAVWNGS V +L+NTR+QK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 61  IMRSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQ 120
           +++S+VTFKD+ P+GGVSN+L+HE +ML+GAAIFNMKRL+GRVDTDPVVHA K+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 121 TLDIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRF 180
           TLDIGVRPF+AALVNN WRSTTPEEVLAIF+VEL++MAE QLKRP+RNVVLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 181 QLTRVERACAMAGFQV-RLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYC 240
           QLTR ERACAMAG  V RLMPEPTA+ALLYAQQQQ   HD+MGSGSE++A+IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 241 DAAVSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVA 300
           D AV+A  GGVSQI+ALAGSPIGGED+LQNT++++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 301 TQDAIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVG 360
            QDAIH+L+ Q +V+I V LGNG+K+ KVL R EFE VN KVFE+CE LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 361 IEDLSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPF 420
             D+ D+++VGGCSYIPK+R ++  + KK  +Y+G++PLEAAV GAALEGA+ SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 421 GSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGE 480
           GSLDLL+IQATPLA+G+RA+GN FIP+IP+NT +PARK+L FTTVQDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 481 ETRAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYME 540
               EENHLLGYFK++GIPPAPKGVPEI +CMDID+SN LRV A   +PG+  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 541 VKMPTVDDGHGWCAEALHGKYGATMELVTLRKK 571
           V+MPTVDDGHGWCA+AL+ KYGAT++L+TL++K
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRK 562

BLAST of Pay0015296 vs. TAIR 10
Match: AT2G32120.2 (heat-shock protein 70T-2 )

HSP 1 Score: 828.9 bits (2140), Expect = 2.4e-240
Identity = 403/573 (70.33%), Postives = 488/573 (85.17%), Query Frame = 0

Query: 1   MAEPIYTVASDSETTGEDRTTS--TFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQK 60
           MAE  YTVASDSE TGE++++S  + PEIA+GIDIGTSQCSIAVWNGS V +L+NTR+QK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 61  IMRSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQ 120
           +++S+VTFKD+ P+GGVSN+L+HE +ML+GAAIFNMKRL+GRVDTDPVVHA K+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 121 TLDIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRF 180
           TLDIGVRPF+AALVNN WRSTTPEEVLAIF+VEL++MAE QLKRP+RNVVLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 181 QLTRVERACAMAGFQV-RLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYC 240
           QLTR ERACAMAG  V RLMPEPTA+ALLYAQQQQ   HD+MGSGSE++A+IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 241 DAAVSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVA 300
           D AV+A  GGVSQI+ALAGSPIGGED+LQNT++++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 301 TQDAIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVG 360
            QDAIH+L+ Q +V+I V LGNG+K+ KVL R EFE VN KVFE+CE LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 361 IEDLSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPF 420
             D+ D+++VGGCSYIPK+R ++  + KK  +Y+G++PLEAAV GAALEGA+ SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 421 GSLDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGE 480
           GSLDLL+IQATPLA+G+RA+GN FIP+IP+NT +PARK+L FTTVQDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 481 ETRAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYME 540
               EENHLLGYFK++GIPPAPKGVPEI +CMDID+SN LRV A   +PG+  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 541 VKMPTVDDGHGWCAEALHGKYGATMELVTLRKK 571
           V+MPTVDDGHGWCA+AL+ KYGAT++L+TL++K
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRK 562

BLAST of Pay0015296 vs. TAIR 10
Match: AT3G12580.1 (heat shock protein 70 )

HSP 1 Score: 285.8 bits (730), Expect = 7.6e-77
Identity = 179/511 (35.03%), Postives = 285/511 (55.77%), Query Frame = 0

Query: 28  AVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIMRSYVTFKDDTPSGGVSNELSHEYDMLS 87
           A+GID+GT+   + VW    VE++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 88  GAAIFNMKRLIGRVDTDPVVHACKS-LPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLA 147
              +F+ KRLIGR  +DP V A KS  PF V +   G +P +        +  + EE+ +
Sbjct: 69  TNTVFDAKRLIGRRYSDPSVQADKSHWPFKVVS-GPGEKPMIVVNHKGEEKQFSAEEISS 128

Query: 148 IFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGFQV-RLMPEPTAVAL 207
           + +++++ +AE  L  P++N V+T+P  F+  Q    + A  ++G  V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAI 188

Query: 208 LYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAAVSAIGGGVSQIRALAG-SPIGGEDL 267
            Y   ++ +      S  EK  LIF++G G  D ++  I  G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAS------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 268 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSSVEINVSLGNGSK 327
               + + +   +           R +  LR A + A   LS   Q+++EI+ SL  G  
Sbjct: 249 DNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEID-SLFEGID 308

Query: 328 LCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKIRKLLME 387
               ++R  FE +N  +F KC   V++CL DA++    + DVV+VGG + IPK+++LL +
Sbjct: 309 FYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQD 368

Query: 388 ISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRADGNSFI 447
               K L + I+P EA   GAA++ AI SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQAAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 448 PIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPKGV 507
            +IP+NTT+P +KE +F+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPAP+GV
Sbjct: 429 VLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 508 PEITICMDIDSSNMLRVLAGAALPGAQHPAT 534
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Pay0015296 vs. TAIR 10
Match: AT1G16030.1 (heat shock protein 70B )

HSP 1 Score: 282.7 bits (722), Expect = 6.5e-76
Identity = 181/517 (35.01%), Postives = 282/517 (54.55%), Query Frame = 0

Query: 22  STFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIMRSYVTFKDDTPSGGVSNELSH 81
           +T  E A+GID+GT+   + VW    VE++ N +  +   SYV F D      + +   +
Sbjct: 2   ATKSEKAIGIDLGTTYSCVGVWMNDRVEIIPNDQGNRTTPSYVAFTDTERL--IGDAAKN 61

Query: 82  EYDMLSGAAIFNMKRLIGRVDTDPVVHA-CKSLPFLVQTLDIGVRPFVAALVNNVWRSTT 141
           +  +     +F+ KRLIGR  +DP V +     PF V +   G +P +     N  +  +
Sbjct: 62  QVALNPQNTVFDAKRLIGRKFSDPSVQSDILHWPFKVVS-GPGEKPMIVVSYKNEEKQFS 121

Query: 142 PEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGFQV-RLMPE 201
           PEE+ ++ +V++K +AE  L R ++N V+T+P  F+  Q    + A A++G  V R++ E
Sbjct: 122 PEEISSMVLVKMKEVAEAFLGRTVKNAVVTVPAYFNDSQRQATKDAGAISGLNVLRIINE 181

Query: 202 PTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAAVSAIGGGVSQIRALAG-SP 261
           PTA A+ Y   ++           EK  LIF++G G  D ++  I  GV +++A AG + 
Sbjct: 182 PTAAAIAYGLDKKGT------KAGEKNVLIFDLGGGTFDVSLLTIEEGVFEVKATAGDTH 241

Query: 262 IGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSSVEINVS 321
           +GGED     + + +               R +  LR A + A   LS   Q+++EI+ S
Sbjct: 242 LGGEDFDNRLVNHFVAEFRRKHKKDIAGNARALRRLRTACERAKRTLSSTAQTTIEID-S 301

Query: 322 LGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKI 381
           L  G      +SR  FE +N  +F KC + V++ L DA++    + DVV+VGG + IPKI
Sbjct: 302 LHEGIDFYATISRARFEEMNMDLFRKCMDPVEKVLKDAKLDKSSVHDVVLVGGSTRIPKI 361

Query: 382 RKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRA 441
           ++LL +    K L + I+P EA   GAA++ AI +G G      DLL +   PL++G+  
Sbjct: 362 QQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGSE-KVQDLLLLDVAPLSLGLET 421

Query: 442 DGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIP 501
            G     +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  R  +N+LLG F++ GIP
Sbjct: 422 AGGVMTVLIPRNTTVPCKKEQVFSTYADNQPGVLIQVYEGERARTRDNNLLGTFELKGIP 481

Query: 502 PAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPAT 534
           PAP+GVP+I +C DID++ +L V A     G ++  T
Sbjct: 482 PAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQIT 507

BLAST of Pay0015296 vs. TAIR 10
Match: AT5G02500.1 (heat shock cognate protein 70-1 )

HSP 1 Score: 278.9 bits (712), Expect = 9.3e-75
Identity = 177/511 (34.64%), Postives = 280/511 (54.79%), Query Frame = 0

Query: 28  AVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIMRSYVTFKDDTPSGGVSNELSHEYDMLS 87
           A+GID+GT+   + VW    VE++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 88  GAAIFNMKRLIGRVDTDPVVHA-CKSLPFLVQTLDIGVRPFVAALVNNVWRSTTPEEVLA 147
              +F+ KRLIGR  +D  V +  K  PF +Q      +P +        +    EE+ +
Sbjct: 69  VNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQA-GPADKPMIYVEYKGEEKEFAAEEISS 128

Query: 148 IFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGFQV-RLMPEPTAVAL 207
           + +++++ +AE  L   I+N V+T+P  F+  Q    + A  +AG  V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAI 188

Query: 208 LYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAAVSAIGGGVSQIRALAG-SPIGGEDL 267
            Y   ++        S  EK  LIF++G G  D ++  I  G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAT------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 268 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSSVEINVSLGNGSK 327
               + + +   +           R +  LR + + A   LS   Q+++EI+ SL  G  
Sbjct: 249 DNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEID-SLYEGID 308

Query: 328 LCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIEDLSDVVVVGGCSYIPKIRKLLME 387
               ++R  FE +N  +F KC   V++CL DA++    + DVV+VGG + IPK+++LL +
Sbjct: 309 FYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQD 368

Query: 388 ISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSLDLLSIQATPLAIGIRADGNSFI 447
               K L + I+P EA   GAA++GAI SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQGAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 448 PIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETRAEENHLLGYFKIIGIPPAPKGV 507
            +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  R ++N+LLG F++ GIPPAP+GV
Sbjct: 429 TLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 508 PEITICMDIDSSNMLRVLAGAALPGAQHPAT 534
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SKY83.4e-23970.33Heat shock 70 kDa protein 8 OS=Arabidopsis thaliana OX=3702 GN=HSP70-8 PE=1 SV=1[more]
Q9LHA81.1e-7535.03Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1[more]
Q9S9N19.1e-7535.01Heat shock 70 kDa protein 5 OS=Arabidopsis thaliana OX=3702 GN=HSP70-5 PE=2 SV=1[more]
P091891.2e-7435.34Heat shock cognate 70 kDa protein OS=Petunia hybrida OX=4102 GN=HSP70 PE=2 SV=1[more]
P229531.3e-7334.64Heat shock 70 kDa protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP70-1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A1S3AXP00.0e+00100.00heat shock 70 kDa protein 8 OS=Cucumis melo OX=3656 GN=LOC103483715 PE=3 SV=1[more]
A0A5D3D0520.0e+0099.82Heat shock 70 kDa protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A0A0LAC60.0e+0098.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G147740 PE=3 SV=1[more]
A0A6J1GXD41.6e-30092.48heat shock 70 kDa protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111457701 PE=3 S... [more]
A0A6J1C9922.7e-30091.94heat shock 70 kDa protein 8 OS=Momordica charantia OX=3673 GN=LOC111009482 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_008438682.10.0e+00100.00PREDICTED: heat shock 70 kDa protein 8 [Cucumis melo][more]
KAA0049367.10.0e+0099.82heat shock 70 kDa protein 8 [Cucumis melo var. makuwa] >TYK17192.1 heat shock 70... [more]
XP_004134140.10.0e+0098.07heat shock 70 kDa protein 8 [Cucumis sativus] >XP_011650982.1 heat shock 70 kDa ... [more]
XP_038897373.13.9e-30995.27heat shock 70 kDa protein 8 [Benincasa hispida][more]
XP_023527609.18.7e-30193.01heat shock 70 kDa protein 8 isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G32120.12.4e-24070.33heat-shock protein 70T-2 [more]
AT2G32120.22.4e-24070.33heat-shock protein 70T-2 [more]
AT3G12580.17.6e-7735.03heat shock protein 70 [more]
AT1G16030.16.5e-7635.01heat shock protein 70B [more]
AT5G02500.19.3e-7534.64heat shock cognate protein 70-1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00301HEATSHOCK70coord: 167..187
score: 28.47
coord: 502..518
score: 35.4
coord: 27..40
score: 41.3
coord: 393..413
score: 43.48
coord: 421..440
score: 36.79
coord: 361..377
score: 43.76
NoneNo IPR availableGENE3D3.30.420.40coord: 212..390
e-value: 8.5E-79
score: 266.9
NoneNo IPR availableGENE3D3.30.30.30coord: 92..147
e-value: 8.5E-79
score: 266.9
NoneNo IPR availableGENE3D3.30.420.40coord: 29..408
e-value: 8.5E-79
score: 266.9
NoneNo IPR availableGENE3D3.90.640.10Actin; Chain A, domain 4coord: 261..342
e-value: 8.5E-79
score: 266.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR19375:SF101HEAT SHOCK 70 KDA PROTEIN 8coord: 4..570
NoneNo IPR availableCDDcd10170HSP70_NBDcoord: 29..408
e-value: 6.47301E-80
score: 254.075
IPR013126Heat shock protein 70 familyPFAMPF00012HSP70coord: 28..523
e-value: 6.2E-87
score: 292.2
IPR013126Heat shock protein 70 familyPANTHERPTHR19375HEAT SHOCK PROTEIN 70KDAcoord: 4..570
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilyGENE3D2.60.34.10Substrate Binding Domain Of DNAk; Chain A, domain 1coord: 419..530
e-value: 3.8E-34
score: 119.9
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilySUPERFAMILY100920Heat shock protein 70kD (HSP70), peptide-binding domaincoord: 423..522
IPR018181Heat shock protein 70, conserved sitePROSITEPS01036HSP70_3coord: 364..378
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 27..209
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 223..409

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0015296.1Pay0015296.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034620 cellular response to unfolded protein
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0042026 protein refolding
biological_process GO:0009408 response to heat
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0031072 heat shock protein binding
molecular_function GO:0051787 misfolded protein binding
molecular_function GO:0044183 protein folding chaperone
molecular_function GO:0051082 unfolded protein binding