Pay0014851 (gene) Melon (Payzawat) v1

Overview
NamePay0014851
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptiontRNA (guanine(26)-N(2))-dimethyltransferase
Locationchr10: 13154157 .. 13158200 (+)
RNA-Seq ExpressionPay0014851
SyntenyPay0014851
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAATGCAAAATCCAACGATTTCCCGCCATGTTAACTGTAGCGTCAACAACCCTATCTCCTTCCCCGTTTCTTTACATCCAACAAACAAACTCCCATTTCCTCACCTCTCCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAAAATCCCCCAACAATTGTCTCTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGTGTCCTCGCCGCCGCGCTCTACCGAAAATCCAAGGGCCATCTTCGGATTCTCGACGCATTGTGCGGTTGTGGGATCCGGTCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTCTCGAAAGTTGCCGAAAATTTTGGGGATAATCGACGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCCACGGATGGTTTCTCTTCCGGCGGACATCGGCCTTCTCAGTGAGTGCATAATCTTACTCTTTAGTTATCTGCTTTAACCGGTTCATTAAAATCTTGATATGAAATGTCTTATCATTTTAATGTCCTGTACCTATATATAAAGGTGCTGAAGGATTGATAATGGTTATAATTAAATTTAACAATAACTCAATGTTTAAGCTCTTGGGTCAATGAGTGATTAGATATGGTATAGAGTTGATATGTTTGAGCTGTATCATGTTCTAATGTCCATCTTTTCCCCACTTAATATTGATTTTCACTTTTTTTTTTGAGAATTTCATATAAATTTTCAAGTCGACAAGTGTGGTGGAATATTATTCTTATAATCAAATTTATCATAAATCATCAACTTAAATTCTTGGGTCAATCATTTAATAAAAGGATTTTAAATGATTAAGTATAGTTATTTTAGTTTTGAGTTTTAGTAGTTGAATTTGGTGTTAGCTCCCCATTAATGCTGTTAGTTACTTGTTGAATCTTTGTCTCTAGGACTAAGCAAATCGACTTGTAACTGATAGATTACTTAAAACTGACTTTTTAGCTGAATGTTTTAAGAAATTGAACTGAACTAGCTAAATTGACAAAACCAGCTGAAATTGAATAAGGGTTGCTATTTGCTAAAAATAACAAACTTTATCCAAATCATTTTGATTGTTTTTGGTTTTGACTTCCATCCTTACCTGTACTAAGTTTTAAGCCTAAAAGTGAAATGGTTGCTAAGTATATGAATATAGGTGGATAATTATATATGAAGATGCGGTAATTAAAATTCTTTTTTCTCCTTTTTCTTGTCTCTCTTTTCTTCTTTCCATTTCTCTCTAGATCTTTAGCTGCATATGGAGCATATATACGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTACTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTTCTAAAAACAGGTGATAGTTGATGAGTTTAGCTTTGTCTGCTAGTGTTGGATGTATCTAGTATGATCATTGATGGTGATTGATCGAAGCTCTCACTATTCAATATCAGGCATTATGGTTTTGTCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTAAGATTATGACTACTATGACGATGCTTGAAATGATTCATGAATATGCTTGGATTTGCTGTTTTCTTATTCATTTGGGTTCTTGCTTTCTAGTGTATTTACTTGCAACATTCAATCTTGTAAGAGTCTCGAGATACATTAAATTTTTTTGACTTGTTAGGTTTCTGAGTCACTAGTGGTCTCAGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATTGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGATGCAGATGCTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCCAAATTTTATTTATTCTTCTCGTGATTTAATATTTTATCTTTCTTTTTGATCCAATAATGCAAGAAGTGATCTATCAAGGCCCCCCTTCTCTATGCTTAGGTGGCAAGTCGGGCAAAAGTAAACTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGTGAGAATACTGGTAGTTAAGATGTGATAAATTGTGTGATGCATATTATTCCACATGGCTCAACTGTTTTTGTTGTTGTGATATTCTCTTGAATGCTTGAAAGTAGAGAAATGTTGTTGTATATATTAAACCACCAATTCACCCAAGAGCTTAAGCTGGTGATTGAAGACAAATTTAATTATATATCACTAACAGTATACATGAGACACAAGGCACAAATACGATAGGACACAAAAACAATGATGGGTTATTCTCTATGAGTAGGAAATGGCCATGGCGTGGATATGATTACTAAAATTTATATGTTTTTAAGCTATATCGTTTTTATTCAATTTGAAGTCTGTAAGCTTTGTGTGTATATATATCTATTTGAAAAATTAAGTTTGGATATGGTTTGCTTCAATATTTATTATTATTATTTTGCATTATCACTATACATATCTAAGTATTAATCTTAACAAATATTTGACACTAGTTGAACAAGTGCAAAAACTTGTATTCAATTTGTTCAACTACTGTCCAACAGTTGTATACTTGTCTTGTGGGTACAAGGATATCACACGTGTTCATCATGTTTTGGAGTGTCCAATAAGCGTCGGACTTGGACGATTTTTCCAAACTATAATGTTTTTTTTTTTTTGCTTGGAATGCAGATGCTTGAAAAGAAAACAATTCTCTCTTTTCTCTCTTCTCTCTTTCTGAGGGTTTTGGTTGTGGCAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGACTGCATCAGAATTGCTCAACTGCAGCAAAATTGTCTTGGTGTGCATCAAGTCACATGAATGTTGTAGCCATAGCTTGGGGCTATGGAAACTACAACCCTTCATACGCAAACAAGAACGAAATGAAACCAATGTAAGCATCCATCTCTCTTTAGTTATCTCATTTTGAAAATTGATGAAGATAATTATGGGTCAAATGCGTTAAAAGTGGTAAAGAACCATGTCCGATGCTCTCAAGAAATTTGCGTTGCAGGTTTTGGTGACGAGAGACAACCAAGTTAGATCGAAAAACTTTAACTTTCTGTTGCATTCTGTCAAAGATAGACAAGAAATGAGCACATACAGACACAAGACAGGGTCACAGAGACACCATGCCATTTCATTTAAAGTGTCGTATTGATGCTTTATAGAAGTAGATAACTAAAATGTTTATATCAAATTCATGCATATTAAGTGTAAATTAATTAGTTTATTATATGAACCAGGATGAGATCACGGAAGCAGTTGAACTTCGATTTTGAAGAACGAAAGCTGTGCGGAGCTCAAGTACGGGACTTGATAAAGAACCTTCCCAGACTTGTTAGCCACAACTCTTTATTTATACTTCTTATAGAGCCATGATGTGATGTGATGTGATGTTCTCATCTTAGGCACAAAATGAAATATTAAGCTAGAAAGTGCAGGCCAATAGTAAAATCTACCTTCTCAGTTTTTAGTTGAACATTGCTGACAAGACTAACCTAATATGTAGGGATATATTATGAAATTTTCAATTTTTGTGTGCAACAGGAAAAGTAACTCAATCATAAATGGCATAGTCTACCTCCTTGTAGGTTCAAGATTTGATC

mRNA sequence

GAAAATGCAAAATCCAACGATTTCCCGCCATGTTAACTGTAGCGTCAACAACCCTATCTCCTTCCCCGTTTCTTTACATCCAACAAACAAACTCCCATTTCCTCACCTCTCCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAAAATCCCCCAACAATTGTCTCTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGTGTCCTCGCCGCCGCGCTCTACCGAAAATCCAAGGGCCATCTTCGGATTCTCGACGCATTGTGCGGTTGTGGGATCCGGTCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTCTCGAAAGTTGCCGAAAATTTTGGGGATAATCGACGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCCACGGATGGTTTCTCTTCCGGCGGACATCGGCCTTCTCAATCTTTAGCTGCATATGGAGCATATATACGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTACTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTTCTAAAAACAGGCATTATGGTTTTGTCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTTTCTGAGTCACTAGTGGTCTCAGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATTGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGATGCAGATGCTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGGGCAAAAGTAAACTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGACTGCATCAGAATTGCTCAACTGCAGCAAAATTGTCTTGGTGTGCATCAAGTCACATGAATGTTGTAGCCATAGCTTGGGGCTATGGAAACTACAACCCTTCATACGCAAACAAGAACGAAATGAAACCAATGTTTTGGTGACGAGAGACAACCAAGTTAGATCGAAAAACTTTAACTTTCTGTTGCATTCTGTCAAAGATAGACAAGAAATGAGCACATACAGACACAAGACAGGGTCACAGAGACACCATGCCATTTCATTTAAAGTGTCGTATTGATGCTTTATAGAAGTAGATAACTAAAATGTTTATATCAAATTCATGCATATTAAGTGTAAATTAATTAGTTTATTATATGAACCAGGATGAGATCACGGAAGCAGTTGAACTTCGATTTTGAAGAACGAAAGCTGTGCGGAGCTCAAGTACGGGACTTGATAAAGAACCTTCCCAGACTTGTTAGCCACAACTCTTTATTTATACTTCTTATAGAGCCATGATGTGATGTGATGTGATGTTCTCATCTTAGGCACAAAATGAAATATTAAGCTAGAAAGTGCAGGCCAATAGTAAAATCTACCTTCTCAGTTTTTAGTTGAACATTGCTGACAAGACTAACCTAATATGTAGGGATATATTATGAAATTTTCAATTTTTGTGTGCAACAGGAAAAGTAACTCAATCATAAATGGCATAGTCTACCTCCTTGTAGGTTCAAGATTTGATC

Coding sequence (CDS)

ATGTTAACTGTAGCGTCAACAACCCTATCTCCTTCCCCGTTTCTTTACATCCAACAAACAAACTCCCATTTCCTCACCTCTCCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAAAATCCCCCAACAATTGTCTCTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGTGTCCTCGCCGCCGCGCTCTACCGAAAATCCAAGGGCCATCTTCGGATTCTCGACGCATTGTGCGGTTGTGGGATCCGGTCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTCTCGAAAGTTGCCGAAAATTTTGGGGATAATCGACGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCCACGGATGGTTTCTCTTCCGGCGGACATCGGCCTTCTCAATCTTTAGCTGCATATGGAGCATATATACGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTACTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTTCTAAAAACAGGCATTATGGTTTTGTCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTTTCTGAGTCACTAGTGGTCTCAGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATTGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGATGCAGATGCTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGGGCAAAAGTAAACTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGACTGCATCAGAATTGCTCAACTGCAGCAAAATTGTCTTGGTGTGCATCAAGTCACATGA

Protein sequence

MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPMADCIRIAQLQQNCLGVHQVT
Homology
BLAST of Pay0014851 vs. ExPASy Swiss-Prot
Match: B6YUU9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) OX=523850 GN=trm1 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.6e-31
Identity = 114/373 (30.56%), Postives = 184/373 (49.33%), Query Frame = 0

Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ VLA     K+ G  R+LDAL   GIR +RY +E+ A  +  ND N  
Sbjct: 27  FYNPLMALNRDISVLAL----KALGSRRVLDALSATGIRGIRYALETPAGEIWLNDINPN 86

Query: 124 NRRVIVKNL-----------SKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVD 183
             R+IV N+            K+A   G+ +  +VTN DANR+M E +    +FDF+D+D
Sbjct: 87  AFRLIVDNVKLNFSGELEIEGKMAVLEGE-KTLVVTNADANRLMNEQF---RYFDFVDLD 146

Query: 184 SFGSDSSFLRPAINALKLDGLLYVTSTD-GFSSGGHRPSQSLAAYGAYIRPMPFSNEIGL 243
            FGS   FL  A+ A+K  G+L +T+TD G   G +R +  L  Y A        +E GL
Sbjct: 147 PFGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYR-NACLRKYLAEPIRGELCHEAGL 206

Query: 244 RMLIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGN 303
           R+LIG + R A+        L +YY  H   FR  L++  G  + ++    + Y  +   
Sbjct: 207 RILIGTLVRYAAKYDLGVEVLLAYYRDH--YFRAFLQLRNGAKTADKSMKQLGYLYQ--- 266

Query: 304 SQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQG 363
                  E G+      ++ +       GPLW+GPL + +++E++  L+ +   +G+   
Sbjct: 267 ------DESGKFE--FENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHP-LGS--- 326

Query: 364 KDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIAS 423
           K  +K L  + DE +  +P  Y     VA R  +    L+ ++  + + GY A+R+H + 
Sbjct: 327 KKTQKFLGLLKDELE--VPFFY-DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSP 370

Query: 424 NAIKTNCPMADCI 425
            A+KT+ P  + +
Sbjct: 387 TALKTDAPFEEVL 370

BLAST of Pay0014851 vs. ExPASy Swiss-Prot
Match: P81554 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=trm1 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.8e-31
Identity = 108/369 (29.27%), Postives = 171/369 (46.34%), Query Frame = 0

Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ VL   + +       +LDAL   GIR +R+ +E+ A+ +  ND N+ 
Sbjct: 29  FYNPRMAPNRDVVVLLLNVLKPK----IVLDALSATGIRGIRFALETPAEEIWMNDINEL 88

Query: 124 NRRVIVKNL-----SKVAEN-----FGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 183
              ++ KN+       + EN     F   +  ++ N DANR+M E   K  +F FID+D 
Sbjct: 89  AYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAE---KHRYFHFIDLDP 148

Query: 184 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRM 243
           FGS   FL  A+ ++K  G+L VT+TDG    G  P   L  Y A        +E+G R+
Sbjct: 149 FGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRI 208

Query: 244 LIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKN---RHYGFVSYCEKCG 303
           L+G +AR A+        L +YY  H   FR  +++  G    +    + G+V + EK G
Sbjct: 209 LVGVIARYAAKYDLGMEVLLAYYKDH--YFRAFVKLKDGAKKGDETLENLGYVYFDEKTG 268

Query: 304 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQ 363
             +                S +S      GPLW+GPL +   + ++L L      + N  
Sbjct: 269 RFEV-------------EKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVAN-- 328

Query: 364 GKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIA 420
            +++ KLL  + +E D  L   +     +  R K+    L  ++ E+   GY A+R+H +
Sbjct: 329 YREVLKLLHMLHEELDIPL---FYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFS 370

BLAST of Pay0014851 vs. ExPASy Swiss-Prot
Match: Q9V1P3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=trm1 PE=3 SV=2)

HSP 1 Score: 136.0 bits (341), Expect = 1.1e-30
Identity = 101/369 (27.37%), Postives = 178/369 (48.24%), Query Frame = 0

Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ V+   + +       +LDAL   GIR +R+ +E+ A+ +  ND +++
Sbjct: 27  FYNPRMALNRDIAVVLLNVLKPR----IVLDALSATGIRGIRFALETPAEEIWMNDISED 86

Query: 124 NRRVIVKNL-----SKVAENFG-----DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 183
              ++ KN+      ++ E+ G       +  +V + DANR+M E   K  +F FID+D 
Sbjct: 87  AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAE---KHRYFHFIDLDP 146

Query: 184 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRM 243
           FGS   FL  A+ ++K  G+L +T+TDG    G  P   +  Y A        +E+G R+
Sbjct: 147 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 206

Query: 244 LIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKN---RHYGFVSYCEKCG 303
           L+G VAR A+        + +YY  H   FR  +++  G    +    + G++ + E  G
Sbjct: 207 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 266

Query: 304 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQ 363
                   E+ +   P   +         GP+W+GPL S + +E++L +++Q   +   +
Sbjct: 267 K------FEVERSFLPSKPN-------AYGPVWLGPLKSQEIVEEMLEISQQ---LSLAR 326

Query: 364 GKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIA 420
            K   KLL  + DE D  L   +     +  R K+ +  +  +++E+   GY ASR+H +
Sbjct: 327 KKQAVKLLKILKDELDVPL---FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 367

BLAST of Pay0014851 vs. ExPASy Swiss-Prot
Match: Q97ZH0 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=trm1 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.4e-30
Identity = 106/361 (29.36%), Postives = 188/361 (52.08%), Query Frame = 0

Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVES-EADFVLANDAND 123
           F+  +    RDL V+  ++ +       I+DAL   GIR +RY VES +++ ++ ND N 
Sbjct: 35  FYNPKMTFNRDLSVIVVSILKPK----IIVDALSATGIRGIRYYVESWKSEQLILNDKNP 94

Query: 124 ENRRVIVKNLSKVAENFG-DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFL 183
               +I  N    A+N G +N +  + N DAN ++ E        D+ID+D FGS + F+
Sbjct: 95  NATSLIQIN----AKNNGIENAK--IYNKDANALLYEVK-----SDYIDIDPFGSPAPFI 154

Query: 184 RPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVARE 243
             ++NA   +G++  T+TD     G  P+     Y A    +  S E+GLR+LIG + RE
Sbjct: 155 LSSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIRE 214

Query: 244 ASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISK----NRHYGFVSYCEKCGNSQAFSW 303
           A++L     PLFS+Y+ +   +R+ +R+  G        N+H  +   C +CG  Q F  
Sbjct: 215 AAILEKTVYPLFSFYADY--YYRLFVRVENGARKADDNINKHLKYFGECPRCG-FQTFV- 274

Query: 304 SELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKL 363
            E  +  CP       E   + GPL+IGPL+S ++++ I+ L   + ++ +     ++KL
Sbjct: 275 EENCKTKCPV----CGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTS--FNRIQKL 334

Query: 364 LMQMLDESDSKLPVGYIKMDEVASRAKVNS-PPLSTMMSEMIKAGYAASRSHIASNAIKT 418
           L  +  E +++    +  + ++AS+ K+++ PP+ +++  +      ASR+H A   I+T
Sbjct: 335 LNVI--EKEARFKNVFYNISKLASKLKISAIPPIESILECL----GDASRTHFAPTGIRT 364

BLAST of Pay0014851 vs. ExPASy Swiss-Prot
Match: C5A6N2 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) OX=593117 GN=trm1 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 5.8e-29
Identity = 114/379 (30.08%), Postives = 177/379 (46.70%), Query Frame = 0

Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ VLA     K+    R+LDAL   GIR LRY +E+ A+ V  ND N++
Sbjct: 27  FYNPVMALNRDISVLAV----KALSPRRVLDALSATGIRGLRYSLETSAEEVWMNDINED 86

Query: 124 NRRVIVKNLSKVAENFGD--------------NRRWLVTNCDANRVMTEFYLKKDFFDFI 183
             R+I+KNL     NFG                +R +V   DAN++M+E +    +FDFI
Sbjct: 87  AFRLILKNLEL---NFGSKPVLANGRAELGLPEKRLVVNLGDANKLMSENF---RYFDFI 146

Query: 184 DVDSFGSDSSFLRPAINALKLDGLLYVTSTD-GFSSGGHRPSQSLAAYGAYIRPMPFSNE 243
           D+D FGS   FL  A+ +++  G L +T+TD G   G +R +         IR     +E
Sbjct: 147 DLDPFGSPVEFLDSALRSVRRRGFLGLTATDTGVLCGAYRRACRRKYLAEPIRG-ELCHE 206

Query: 244 IGLRMLIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEK 303
            GLR+LIG + R A+        L +YY  H   FRV LR+  G    +     + Y   
Sbjct: 207 AGLRILIGTIVRYAAKYDLGVEVLLAYYRDH--YFRVFLRLRSGAKKADESVELLGYL-- 266

Query: 304 CGNSQAFSW-SELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAK-QWEWI 363
                   W  E G+         +       GP+W+GPL +  ++E+++ L K   E I
Sbjct: 267 --------WQDETGKFK--YERDFLPGKRGAYGPMWLGPLKNQAFLEELVRLTKTSEESI 326

Query: 364 GNGQGKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASR 423
            + +      LL++ LD     +P  Y     +A +  +    +S ++  +   G+ A+R
Sbjct: 327 SHRKTFQFLSLLVEELD-----VPFHY-DTHALARKNGLEVVKISRVIETLHSLGFEATR 374

Query: 424 SHIASNAIKTNCPMADCIR 426
           +H +  +IKTN P    +R
Sbjct: 387 THFSPTSIKTNAPFDVVLR 374

BLAST of Pay0014851 vs. ExPASy TrEMBL
Match: A0A1S3C509 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 4.6e-255
Identity = 440/440 (100.00%), Postives = 440/440 (100.00%), Query Frame = 0

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           ADCIRIAQLQQNCLGVHQVT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

BLAST of Pay0014851 vs. ExPASy TrEMBL
Match: A0A5D3DFE8 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold530G00120 PE=3 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 4.6e-255
Identity = 440/440 (100.00%), Postives = 440/440 (100.00%), Query Frame = 0

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           ADCIRIAQLQQNCLGVHQVT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

BLAST of Pay0014851 vs. ExPASy TrEMBL
Match: A0A0A0KP99 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G220920 PE=3 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 3.1e-243
Identity = 420/440 (95.45%), Postives = 428/440 (97.27%), Query Frame = 0

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVA  TLSPSPFL+I QTNSHFLTS SIHLFQFSHSNK   NNCLSNNRTERGLDFDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLS+VAEN GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           A+CIRIAQLQ+NCLG HQVT
Sbjct: 421 AECIRIAQLQRNCLGAHQVT 440

BLAST of Pay0014851 vs. ExPASy TrEMBL
Match: A0A1S3C4F5 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 1.8e-230
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 400
           MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 399

BLAST of Pay0014851 vs. ExPASy TrEMBL
Match: A0A6J1FWD4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447941 PE=3 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 4.1e-227
Identity = 392/441 (88.89%), Postives = 416/441 (94.33%), Query Frame = 0

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTV   TLSPSPFL  QQT SH  +S +IHLFQFS S KK  NNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENR VIVKNLS+VAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYC+KCGNSQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIA-QLQQNCLGVHQVT 441
           A+CIRIA +LQQ+CLGVHQ T
Sbjct: 421 AECIRIALELQQSCLGVHQAT 441

BLAST of Pay0014851 vs. NCBI nr
Match: XP_008456934.1 (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo] >XP_008456936.1 PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo] >TYK22417.1 tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 889.8 bits (2298), Expect = 9.5e-255
Identity = 440/440 (100.00%), Postives = 440/440 (100.00%), Query Frame = 0

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           ADCIRIAQLQQNCLGVHQVT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

BLAST of Pay0014851 vs. NCBI nr
Match: XP_011655038.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >KGN50729.1 hypothetical protein Csa_005829 [Cucumis sativus])

HSP 1 Score: 850.5 bits (2196), Expect = 6.4e-243
Identity = 420/440 (95.45%), Postives = 428/440 (97.27%), Query Frame = 0

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVA  TLSPSPFL+I QTNSHFLTS SIHLFQFSHSNK   NNCLSNNRTERGLDFDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLS+VAEN GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           A+CIRIAQLQ+NCLG HQVT
Sbjct: 421 AECIRIAQLQRNCLGAHQVT 440

BLAST of Pay0014851 vs. NCBI nr
Match: XP_008456937.1 (PREDICTED: tRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferase isoform X2 [Cucumis melo])

HSP 1 Score: 808.1 bits (2086), Expect = 3.7e-230
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 400
           MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 399

BLAST of Pay0014851 vs. NCBI nr
Match: XP_038892632.1 (tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 805.8 bits (2080), Expect = 1.8e-229
Identity = 395/441 (89.57%), Postives = 421/441 (95.46%), Query Frame = 0

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           ML+V   T SPSPFL+  QTNSH L++ +IHLFQFSHSNK S NNCL NNRTERGL+FDI
Sbjct: 1   MLSVLPRTPSPSPFLHSPQTNSHSLSASTIHLFQFSHSNKNSLNNCLPNNRTERGLEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           G TFFRHESATGRDLGVLAAALYRKSKGHLR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GGTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLS+VAE+ GD RRW+VTNCDANRVMT FYL+KDFFDFID+DSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAEDSGDARRWMVTNCDANRVMTGFYLQKDFFDFIDIDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGR+ KNRHYGF+SYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGHYATPLFSYYSYHGPVFRVMLRINRGRVYKNRHYGFISYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVS+SLVVSGPLW GPLHSADYIE++L+LAKQW+WIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSDSLVVSGPLWTGPLHSADYIENMLSLAKQWKWIGNGQGKDLEKLLGQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MMDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIA-QLQQNCLGVHQVT 441
           A+CIRIA +LQQNCLG HQVT
Sbjct: 421 AECIRIALELQQNCLGFHQVT 441

BLAST of Pay0014851 vs. NCBI nr
Match: XP_022943113.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata])

HSP 1 Score: 797.0 bits (2057), Expect = 8.4e-227
Identity = 392/441 (88.89%), Postives = 416/441 (94.33%), Query Frame = 0

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTV   TLSPSPFL  QQT SH  +S +IHLFQFS S KK  NNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENR VIVKNLS+VAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYC+KCGNSQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIA-QLQQNCLGVHQVT 441
           A+CIRIA +LQQ+CLGVHQ T
Sbjct: 421 AECIRIALELQQSCLGVHQAT 441

BLAST of Pay0014851 vs. TAIR 10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 553.9 bits (1426), Expect = 1.2e-157
Identity = 270/430 (62.79%), Postives = 337/430 (78.37%), Query Frame = 0

Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCL--SNNRTERGLDF 60
           +LT++  TLS S F  + ++ +    SP    F+   S K S +  L  S  + ER L+F
Sbjct: 2   LLTLSPKTLSSSSFFTVHKSQNPKCKSPDSCRFK---SLKCSFDRILVKSEVQIERNLEF 61

Query: 61  DIGDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLAN 120
           + G+TFFRHESA GRDLGVL+A LY++S G LR+LDA+CGCGIRSLRYLVE+EADFV+AN
Sbjct: 62  ETGETFFRHESARGRDLGVLSATLYKRSNGSLRVLDAMCGCGIRSLRYLVEAEADFVMAN 121

Query: 121 DANDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDS 180
           DAND+NRRVI  NLSKV    GD RRW+VT+  AN+ M E Y+  DFFD ID+DSFGSDS
Sbjct: 122 DANDDNRRVITDNLSKVERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDS 181

Query: 181 SFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGV 240
           SFLR A NAL+L GLLY+TSTDG+SSGGHRP  SLAAYGA+IRPMPF NEIGLRMLIGG 
Sbjct: 182 SFLRDAFNALRLGGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGA 241

Query: 241 AREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWS 300
            REA++LGY+ TPLFSYYSYHGPVFRVMLR++RG++ ++R+YGFV++C  CG+S    + 
Sbjct: 242 VREAALLGYHVTPLFSYYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLRFD 301

Query: 301 ELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL 360
           ELG M CPC+D++ S SLVVSGP+W+GPLH A Y+ ++L LAK+W W+  G G DL+KLL
Sbjct: 302 ELGLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLL 361

Query: 361 MQMLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNC 420
             M++ESD +LP GY KMDE+ASRAK+NSPPL  MMS ++K GYAASRSHI  NA+KT+C
Sbjct: 362 SIMIEESDPRLPPGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDC 421

Query: 421 PMADCIRIAQ 429
           PM+  +RIA+
Sbjct: 422 PMSHFVRIAK 428

BLAST of Pay0014851 vs. TAIR 10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 87.8 bits (216), Expect = 2.3e-17
Identity = 85/355 (23.94%), Postives = 148/355 (41.69%), Query Frame = 0

Query: 91  RILDALCGCGIRSLRYLVESEA-DFVLANDANDENRRVIVKN--------LSKVAENFGD 150
           ++L+AL   G+R+LRY  E E    V+A D +  +     +N        +SKV  +  D
Sbjct: 123 KVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVAISKVESHHTD 182

Query: 151 NRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDG 210
            R  ++T+                FD +D+D +GS S FL  AI ++   GLL  T+TD 
Sbjct: 183 ARVHMLTH-------------PKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDM 242

Query: 211 FSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAREASVLGYYATPLFSYYSYHGP 270
               G       + YG+Y     + +E+ LR+L+  +   A+    Y  P+ S       
Sbjct: 243 AVLCGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDF-- 302

Query: 271 VFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDS----------- 330
             RV +R+     +       +SY  +C    +F    +G+ S P N+S           
Sbjct: 303 YVRVFVRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGR-SLPKNNSVRYLPAIGPVV 362

Query: 331 -----RVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQMLDES 390
                   +   + GP+W  P+H  +++  IL   K  +        D    ++  + E 
Sbjct: 363 KQDCNHCGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMK--DRYPAYDRISAVLTTVSEE 422

Query: 391 DSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 421
              +P+ ++ +  + +  K  SP  +   S +I A Y  S +H+    +KT+ PM
Sbjct: 423 LLDVPL-FLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPM 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B6YUU91.6e-3130.56tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain N... [more]
P815542.8e-3129.27tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC ... [more]
Q9V1P31.1e-3027.37tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / O... [more]
Q97ZH02.4e-3029.36tRNA (guanine(26)-N(2))-dimethyltransferase OS=Saccharolobus solfataricus (strai... [more]
C5A6N25.8e-2930.08tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus gammatolerans (strai... [more]
Match NameE-valueIdentityDescription
A0A1S3C5094.6e-255100.00tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496... [more]
A0A5D3DFE84.6e-255100.00tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946... [more]
A0A0A0KP993.1e-24395.45tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A1S3C4F51.8e-230100.00tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496... [more]
A0A6J1FWD44.1e-22788.89tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_008456934.19.5e-255100.00PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo]... [more]
XP_011655038.16.4e-24395.45tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tR... [more]
XP_008456937.13.7e-230100.00PREDICTED: tRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferase isoform ... [more]
XP_038892632.11.8e-22989.57tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida][more]
XP_022943113.18.4e-22788.89tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G56330.11.2e-15762.79N2,N2-dimethylguanosine tRNA methyltransferase [more]
AT3G02320.12.3e-1723.94N2,N2-dimethylguanosine tRNA methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 33..367
e-value: 2.9E-74
score: 251.7
NoneNo IPR availablePANTHERPTHR10631:SF9TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASEcoord: 26..431
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 91..197
e-value: 7.66957E-5
score: 39.7207
IPR042296tRNA methyltransferase, Trm1, C-terminalGENE3D3.30.56.70coord: 372..428
e-value: 5.8E-16
score: 59.9
IPR002905tRNA methyltransferase, Trm1PFAMPF02005TRMcoord: 64..426
e-value: 2.9E-65
score: 220.9
IPR002905tRNA methyltransferase, Trm1PANTHERPTHR10631N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASEcoord: 26..431
IPR002905tRNA methyltransferase, Trm1PROSITEPS51626SAM_MT_TRM1coord: 49..427
score: 92.743385
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 52..427

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0014851.2Pay0014851.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002940 tRNA N2-guanine methylation
biological_process GO:0008033 tRNA processing
cellular_component GO:0005634 nucleus
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004809 tRNA (guanine-N2-)-methyltransferase activity
molecular_function GO:0003723 RNA binding