Pay0014469 (gene) Melon (Payzawat) v1

Overview
NamePay0014469
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionN-acyl-L-amino-acid amidohydrolase
Locationchr11: 15313163 .. 15316464 (+)
RNA-Seq ExpressionPay0014469
SyntenyPay0014469
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGAAGGGGGAAGTGGGCTTTTAGCTCTTTGTTGCTCTGAACATCTCATCTTTTCTTCTTCGGTAATCGATGGCCCAACTTTTGGTTATTGTTGAAGTGGGGCTTTTAGCTCAAATTTCATCATTATCCCATCTCCCCAATGTCAATGGCGGAATGGACCTCTGTCGTCTTCTCTTAACCCTCCCTTTCTCTTCTCCATTTCAACTTGTCAGTAGTTATTGTAATTTATTGTACCAAGTGCTTCTGAATCTGAGCCATGAATTTCAAATCTCTCCATTTTCTCCTTTCCATTATAACTCTTTTCTTCTTTCTATCAATTTCTACTTCTGAACAAGACCCATCGTCTCCCTCTGCAATCGTATCCAGGTTCCAACAATACCTTCAGATTAATACCGTCCAACCCAGCCCCCAATACTATGAAGCTGCTGATTTCATCATTTCTCAGGCCAAATCGCTCTCCCTTGAATCTCACACAATTGAATTCGTCGAGGGCAAGCCTCTGATCATCCTCAAATGGCCTGGTTCCAACCTTGATCTTCCTTCCATTCTCCTCAATTCGCATACCGATGTTGTTCCAGCTGAGCATCACAAATGGACGCACCCTCCTCTTGGAGCTCATATCGATTCCCATGGCAATATCTATGCAAGAGGGTCTCAGGACATGAAGTGCGTTGGGATGCAGTACCTTGAGGCTATTCGGCGTTTGAAGGCATCTGGGTTTCAGCCTCTGCGCTCTGTTTATTTATCTTTTGTCCCGGATGAAGAAATTGGTGGCCATGGTGGTGCTGAGAAATTTGCTGAATCCGATGAGTTCAAGAAATTGAATGTCGCCATTGTGCTTGATGAAGGTTCCGAGTGAACCCGTTTTTGCTGATTTTTCTGATGGGTGAGTTTCTTTCATCCTTGTATGCTTCGCATGTTTAGACTAATGTTGTTCGAAATCATCAGGGTTGCCTTCTCCGGGTGAGAATTATAGGGTGTTTTATGGGGAAAAGAGCCCCTGGTGGCTGGTGATCAAGGCGGTGGGAGCCCCAGGTCATGGAGCTAGGCTTTACGATAACACTGCCTTGGAGAATCTTTTCAAAAGCATTGAGACTGTCAGAAGGTTTAGAGCTTCACAGTTTGATTTGATCAAAGCTGGTTTGAAGACTGAAGGAGATGTGGTCTCTGTCAACATGGTCTTCTTAAAATCTGGCATTCCATCTCCTACTGTGAGCTCATCTTTGAAACTTGTTTCTCCATGAATCTTCTAGCATCTAATAGATGAAACTATCATTCTTCATTTGAGTATTAGTTCCATCATTGTATCTGGCAATTTGGCTAGAATTTATTAGTATTGTCCTATTTTTCGTTTGAGTTCCAAGTTGAACTTCAATAGCAATGGCTTAGACAGTTGAAATAATAGAATATTTGGGTTATTATATCAATCAAGAATGTGCGAGTTGAATTTTTTATGTTGGTTTTATCATGTTTGCGGTCATGACAGACTATTTTGTATTCTGTGTCATACAAAAGAATCTTTTTGCTTATGAAACCCTGTTGCCAACATAACCTGATGGCTTACTCTATTTTCTACCAGGGCTTTGTCATGAATCTGCAGCCATCTGAAGCTGAGGCTGGTTTTGACGTTCGCCTCCCACCAACTACAAATCCGGAGTCTCTTGAGAGACGAATTGCAGAGGAATGGGCACCAGCTTCTCGTAATATGACGTTTGAGGTAATAATTTAGGGATCTTTTGATTCTGGCCTAAGAAGCATGGAATACAATCACAGCATGTATTCTAAGGAACAAAGACACAAATATGGACATATATACTCGAATATATATGTTATTATATCTAATTTTCAATGATTTTGCATTATAAATTCATTTTTCTATGTTTAAAATGATAAAATATGTTACAAATGCAGTGTAGAACATCTATCCAACAAATGGTGGAGTGATGGATGTCCAATACATAGCGGATTCAAACTCAAAAATATCTGCTTAAAAATTCTAACCAGTATCACTTATAAAAGAACCTAATACAGAGAAAGATAGAGTGAAGATGAGGAAGACAGAGTCTGATACAAAGGGTTTTTCTGATGTGTTTTACATATTTGTTTTTGTAAGAGTTTAATGGGCTACCTTATTACGGACTATGCTATTCCACTATCACTTTTACAATGTAAATTTTTCCTCTCATATCTTTCCTTGTGCTTGATTGTTGCTCAGTTTAAGCAGAAGGAGTCTATTTACGACAAGTTTGGTAAACCAGCTCTTACTGCTATTGACAAATCCAACCCGTGGTGGAATCTTCTTGAAGAAGCTGTAAGAAATGCGAATGGAAAACTTGCAAACCCAGAAATCTTTCCTGCTTCAACGGATGCTCGATACTTTCGAAATCTTGGCCTCCCTGCAATCGGCTTCTCTCCAATGGTCAACACTCCTATTCTTCTTCATGACCATAATGAGGTGAGAATCTTATTCCTCTGTTCTTCAGTTGAAGCAGAAACTTTTAAGTCGGATGGTAGCATAAATTTTGGTCATAGTTTGCCTTCGCTTAACTTTTTAACCGTACGTTGAGATGTTGATTGATCATAGTAAATTAATTGATAAACTGAGCAGCTAAACCAGTGTATTCCTTTACCTTCGTTTTAGATTTTGGAGTTTTTGGATATGGATCAGGTTGGTCGTTTTTGTGGGCTTGACATTGAACTGCGTTGTTGTATAAGTCATTAACATGTTCATCTGTTCCAGTTTCTAAACCAAGCTGAGTATTTGAAAGGCATTGAGGTGTATGAGTCAATAATCAAGGCTTATGCTTCTTACGACGGTGACAAACGGATGGAGAGCTTCAAAGATGAGTTATAAGAAAGCAGCAAGGCAGTGTTATCCTTCGAGCATTCAGCCACGAGGTTCCGTAGGGATCAGGCAACTCTAGAGCATGGGGTGGGAAGGGATATGATTTTGTTCCCTAATTAATCTACTGGAACATTACTTCGTTGAACTTTGGTTGTGCTTTATTTGCAAGTACCATATAATAATTCAGGCAGCAGAATTTTCTATCAAATAAAGTGACAATAGAATTGGCTTTCGGATAAACTTTTGGTGGCCCTTATGGTTTGTGCCTGTGGGTAAAAAGGGTGAAAATGGGTATGGGCTTACAAAGCCAATTTCTGGTGGGTGGATATATGCATGGAACTTTACGTGGAAATAGCAAATGTATAGATAGAAAAAAGAAAAATAGCAAACTGTTAAATTATTTTGATTTATTGCAAAACTAATTGTCACAATAATTTTTTAAATTTTTTTTGGCTCG

mRNA sequence

GGGAAGGGGGAAGTGGGCTTTTAGCTCTTTGTTGCTCTGAACATCTCATCTTTTCTTCTTCGGTAATCGATGGCCCAACTTTTGGTTATTGTTGAAGTGGGGCTTTTAGCTCAAATTTCATCATTATCCCATCTCCCCAATGTCAATGGCGGAATGGACCTCTGTCGTCTTCTCTTAACCCTCCCTTTCTCTTCTCCATTTCAACTTGTCAGTAGTTATTGTAATTTATTGTACCAAGTGCTTCTGAATCTGAGCCATGAATTTCAAATCTCTCCATTTTCTCCTTTCCATTATAACTCTTTTCTTCTTTCTATCAATTTCTACTTCTGAACAAGACCCATCGTCTCCCTCTGCAATCGTATCCAGGTTCCAACAATACCTTCAGATTAATACCGTCCAACCCAGCCCCCAATACTATGAAGCTGCTGATTTCATCATTTCTCAGGCCAAATCGCTCTCCCTTGAATCTCACACAATTGAATTCGTCGAGGGCAAGCCTCTGATCATCCTCAAATGGCCTGGTTCCAACCTTGATCTTCCTTCCATTCTCCTCAATTCGCATACCGATGTTGTTCCAGCTGAGCATCACAAATGGACGCACCCTCCTCTTGGAGCTCATATCGATTCCCATGGCAATATCTATGCAAGAGGGTCTCAGGACATGAAGTGCGTTGGGATGCAGTACCTTGAGGCTATTCGGCGTTTGAAGGCATCTGGGTTTCAGCCTCTGCGCTCTGTTTATTTATCTTTTGTCCCGGATGAAGAAATTGGTGGCCATGGTGGTGCTGAGAAATTTGCTGAATCCGATGAGTTCAAGAAATTGAATGTCGCCATTGTGCTTGATGAAGGGTTGCCTTCTCCGGGTGAGAATTATAGGGTGTTTTATGGGGAAAAGAGCCCCTGGTGGCTGGTGATCAAGGCGGTGGGAGCCCCAGGTCATGGAGCTAGGCTTTACGATAACACTGCCTTGGAGAATCTTTTCAAAAGCATTGAGACTGTCAGAAGGTTTAGAGCTTCACAGTTTGATTTGATCAAAGCTGGTTTGAAGACTGAAGGAGATGTGGTCTCTGTCAACATGGTCTTCTTAAAATCTGGCATTCCATCTCCTACTGGCTTTGTCATGAATCTGCAGCCATCTGAAGCTGAGGCTGGTTTTGACGTTCGCCTCCCACCAACTACAAATCCGGAGTCTCTTGAGAGACGAATTGCAGAGGAATGGGCACCAGCTTCTCGTAATATGACGTTTGAGTTTAAGCAGAAGGAGTCTATTTACGACAAGTTTGGTAAACCAGCTCTTACTGCTATTGACAAATCCAACCCGTGGTGGAATCTTCTTGAAGAAGCTGTAAGAAATGCGAATGGAAAACTTGCAAACCCAGAAATCTTTCCTGCTTCAACGGATGCTCGATACTTTCGAAATCTTGGCCTCCCTGCAATCGGCTTCTCTCCAATGGTCAACACTCCTATTCTTCTTCATGACCATAATGAGTTTCTAAACCAAGCTGAGTATTTGAAAGGCATTGAGGTGTATGAGTCAATAATCAAGGCTTATGCTTCTTACGACGGTGACAAACGGATGGAGAGCTTCAAAGATGAGTTATAAGAAAGCAGCAAGGCAGTGTTATCCTTCGAGCATTCAGCCACGAGGTTCCGTAGGGATCAGGCAACTCTAGAGCATGGGGTGGGAAGGGATATGATTTTGTTCCCTAATTAATCTACTGGAACATTACTTCGTTGAACTTTGGTTGTGCTTTATTTGCAAGTACCATATAATAATTCAGGCAGCAGAATTTTCTATCAAATAAAGTGACAATAGAATTGGCTTTCGGATAAACTTTTGGTGGCCCTTATGGTTTGTGCCTGTGGGTAAAAAGGGTGAAAATGGGTATGGGCTTACAAAGCCAATTTCTGGTGGGTGGATATATGCATGGAACTTTACGTGGAAATAGCAAATGTATAGATAGAAAAAAGAAAAATAGCAAACTGTTAAATTATTTTGATTTATTGCAAAACTAATTGTCACAATAATTTTTTAAATTTTTTTTGGCTCG

Coding sequence (CDS)

ATGAATTTCAAATCTCTCCATTTTCTCCTTTCCATTATAACTCTTTTCTTCTTTCTATCAATTTCTACTTCTGAACAAGACCCATCGTCTCCCTCTGCAATCGTATCCAGGTTCCAACAATACCTTCAGATTAATACCGTCCAACCCAGCCCCCAATACTATGAAGCTGCTGATTTCATCATTTCTCAGGCCAAATCGCTCTCCCTTGAATCTCACACAATTGAATTCGTCGAGGGCAAGCCTCTGATCATCCTCAAATGGCCTGGTTCCAACCTTGATCTTCCTTCCATTCTCCTCAATTCGCATACCGATGTTGTTCCAGCTGAGCATCACAAATGGACGCACCCTCCTCTTGGAGCTCATATCGATTCCCATGGCAATATCTATGCAAGAGGGTCTCAGGACATGAAGTGCGTTGGGATGCAGTACCTTGAGGCTATTCGGCGTTTGAAGGCATCTGGGTTTCAGCCTCTGCGCTCTGTTTATTTATCTTTTGTCCCGGATGAAGAAATTGGTGGCCATGGTGGTGCTGAGAAATTTGCTGAATCCGATGAGTTCAAGAAATTGAATGTCGCCATTGTGCTTGATGAAGGGTTGCCTTCTCCGGGTGAGAATTATAGGGTGTTTTATGGGGAAAAGAGCCCCTGGTGGCTGGTGATCAAGGCGGTGGGAGCCCCAGGTCATGGAGCTAGGCTTTACGATAACACTGCCTTGGAGAATCTTTTCAAAAGCATTGAGACTGTCAGAAGGTTTAGAGCTTCACAGTTTGATTTGATCAAAGCTGGTTTGAAGACTGAAGGAGATGTGGTCTCTGTCAACATGGTCTTCTTAAAATCTGGCATTCCATCTCCTACTGGCTTTGTCATGAATCTGCAGCCATCTGAAGCTGAGGCTGGTTTTGACGTTCGCCTCCCACCAACTACAAATCCGGAGTCTCTTGAGAGACGAATTGCAGAGGAATGGGCACCAGCTTCTCGTAATATGACGTTTGAGTTTAAGCAGAAGGAGTCTATTTACGACAAGTTTGGTAAACCAGCTCTTACTGCTATTGACAAATCCAACCCGTGGTGGAATCTTCTTGAAGAAGCTGTAAGAAATGCGAATGGAAAACTTGCAAACCCAGAAATCTTTCCTGCTTCAACGGATGCTCGATACTTTCGAAATCTTGGCCTCCCTGCAATCGGCTTCTCTCCAATGGTCAACACTCCTATTCTTCTTCATGACCATAATGAGTTTCTAAACCAAGCTGAGTATTTGAAAGGCATTGAGGTGTATGAGTCAATAATCAAGGCTTATGCTTCTTACGACGGTGACAAACGGATGGAGAGCTTCAAAGATGAGTTATAA

Protein sequence

MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFIISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESIIKAYASYDGDKRMESFKDEL
Homology
BLAST of Pay0014469 vs. ExPASy Swiss-Prot
Match: Q99JW2 (Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.3e-100
Identity = 186/411 (45.26%), Postives = 256/411 (62.29%), Query Frame = 0

Query: 26  QDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFIISQAKSLSLESHTIEFVEGKPLIIL 85
           +DP S    V+ F+QYL+I TVQP+P Y  A  F+  +A+ L L    IE V G  + +L
Sbjct: 4   KDPESEHPSVTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVL 63

Query: 86  KWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLE 145
            WPG+N  LPSILLNSHTDVVP     W H P  A  DS G IYARGSQDMK V +QYLE
Sbjct: 64  TWPGTNPSLPSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLE 123

Query: 146 AIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSPGEN 205
           A+RRLK+ G +  R+++++FVPDEE+GGH G E F +  EF+ L     LDEGL +P + 
Sbjct: 124 AVRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDA 183

Query: 206 YRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETVRRFRASQFDLIKAG--L 265
           + VFY E+SPWW+ + + G PGH +R  ++TA E L K I ++  FR  +   ++A   L
Sbjct: 184 FTVFYSERSPWWVRVTSTGKPGHASRFIEDTAAEKLHKVISSILAFREKERQRLQANPHL 243

Query: 266 KTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTTNPESLERRIAEEWAP 325
           K EG V SVN+  L+ G+        N+ P+   A FD R+ P  + ++ E+++      
Sbjct: 244 K-EGAVTSVNLTKLEGGV------AYNVVPATMSASFDFRVAPDVDMKAFEKQLQRWCQE 303

Query: 326 ASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPASTDA 385
           A   +TFEF Q      KF +P +T  D S+PWW     A +  N  L  PEIFPA+TD+
Sbjct: 304 AGEGVTFEFAQ------KFTEPRMTPTDDSDPWWAAFSGACKAMNLTL-EPEIFPAATDS 363

Query: 386 RYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 435
           RY R +G+PA+GFSPM  TP+LLHDHNE L++  +L+G+++Y  ++ A AS
Sbjct: 364 RYIRAVGIPALGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALAS 400

BLAST of Pay0014469 vs. ExPASy Swiss-Prot
Match: Q6AYS7 (Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 3.5e-98
Identity = 181/409 (44.25%), Postives = 253/409 (61.86%), Query Frame = 0

Query: 28  PSSPSAIVSRFQQYLQINTVQPSPQYYEAADFIISQAKSLSLESHTIEFVEGKPLIILKW 87
           P S    V+ F+QYL+I TVQP+P Y  A  F+  +A+ L L    IE   G  + +L W
Sbjct: 6   PESEHPSVTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTW 65

Query: 88  PGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAI 147
           PG+N  L SILLNSHTDVVP     W H P  A  DS G IYARG+QDMK V +QYLEA+
Sbjct: 66  PGTNPLLHSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAV 125

Query: 148 RRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSPGENYR 207
           RRLK+ G +  R+++++FVPDEE+GGH G E F +  EF+ L     LDEGL +P + + 
Sbjct: 126 RRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFT 185

Query: 208 VFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETVRRFRASQFDLIKAG--LKT 267
           VFY E+SPWW+ + + G PGH +R  ++TA E L K + ++  FR  +   ++A   LK 
Sbjct: 186 VFYSERSPWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHLK- 245

Query: 268 EGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTTNPESLERRIAEEWAPAS 327
           EG V SVN+  L+ G+        N+ P+   A FD R+ P  + ++ E+++      A 
Sbjct: 246 EGAVTSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQEAG 305

Query: 328 RNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPASTDARY 387
             +TFEF Q      KF +P +T  D ++PWW     A +  N  L  PEIFPA+TD+RY
Sbjct: 306 EGVTFEFAQ------KFTEPRMTPTDDTDPWWAAFSGACKEMNLTL-EPEIFPAATDSRY 365

Query: 388 FRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 435
            R +G+PA+GFSPM  TP+LLHDHNE L++A +L+G+++Y  ++ A AS
Sbjct: 366 IRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

BLAST of Pay0014469 vs. ExPASy Swiss-Prot
Match: Q55DP8 (Aminoacylase-1 OS=Dictyostelium discoideum OX=44689 GN=acy1 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.3e-97
Identity = 180/402 (44.78%), Postives = 259/402 (64.43%), Query Frame = 0

Query: 35  VSRFQQYLQINTVQPSPQYYEAADFIISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDL 94
           V+ F+++L+I T  P+P Y  +  F++ +AK  ++  + +    G P++++K  G   +L
Sbjct: 10  VTVFREFLKIRTDHPTPDYESSTKFLVEKAKEYNI-PYEVYRETGTPIVLMKIEGLEPNL 69

Query: 95  PSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASG 154
            ++LLNSH DVVPA H  W   P  A  D  GNI+ RG+QDMKCV MQ+LE  RR+  SG
Sbjct: 70  KTVLLNSHVDVVPAVHDSWKVDPFSAWKDESGNIFGRGTQDMKCVCMQFLEVARRIVQSG 129

Query: 155 FQPLRSVYLSFVPDEEIGGHG-GAEKFAESDEFKKLNVAIVLDEGLPSPGENYRVFYGEK 214
            +  R+++LSFVPDEEIGG G G EKF  +++F++LN+ + LDEGL SP  ++ VFYGE+
Sbjct: 130 QKLKRTLHLSFVPDEEIGGSGKGMEKFVYTEKFRQLNIGLCLDEGLASPTNDFTVFYGER 189

Query: 215 SPWWLVIKAVGAPGHGARLYDNTALENLFKSIETVRRFRASQFDLIKAGL----KTEGDV 274
           +PWW+ I AVG  GHG+R  + TA+E L ++I  +  FR  QF+ +  G     K  GDV
Sbjct: 190 APWWVHITAVGNAGHGSRFIEGTAIEKLMRTINKMLAFRQEQFESLHHGQHECGKKLGDV 249

Query: 275 VSVNMVFLKSGIP--SPTGFVMNLQPSEAEAGFDVRLPPTTNPESLERRIAEEWAPASRN 334
            S+N+  LK+GIP      F  N+ P++AEAGFD+R+PPT N +    +I +EW  A   
Sbjct: 250 TSLNLTVLKAGIPIDHSNNFSYNVIPTQAEAGFDIRIPPTVNLDQFLDQI-KEWT-AEEG 309

Query: 335 MTFEFKQKESIYDKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPASTDARYFR 394
           ++F+F           K  +T +D  N WW   +E+ +  +  L   EIFPA+TD+R+ R
Sbjct: 310 LSFKFA------SYIPKNEMTKLDSDNKWWENFKESCKKMDINLVT-EIFPAATDSRFIR 369

Query: 395 NLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESII 430
           NLG+PA GFSP+ NTPILLHDHNEFLN+  YL+GI+++  II
Sbjct: 370 NLGIPAFGFSPINNTPILLHDHNEFLNEKVYLRGIDIFMGII 401

BLAST of Pay0014469 vs. ExPASy Swiss-Prot
Match: Q6PTT0 (Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 3.0e-97
Identity = 176/413 (42.62%), Postives = 249/413 (60.29%), Query Frame = 0

Query: 24  SEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFIISQAKSLSLESHTIEFVEGKPLI 83
           + + P      V+ F+QYL+I TVQP P Y  A  F    A+ L L    +E   G  + 
Sbjct: 2   TSKGPEEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVT 61

Query: 84  ILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQY 143
           +L WPG+N  L SILLNSHTDVVP     W+H P  A  DS G IY RG+QDMKCV +QY
Sbjct: 62  VLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYTRGAQDMKCVSIQY 121

Query: 144 LEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSPG 203
           LEA++RLK  G +  R+++++FVPDEE+GGH G E F +  EF  L     LDEGL +P 
Sbjct: 122 LEAVKRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRHEFHALRAGFALDEGLANPT 181

Query: 204 ENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETVRRFRASQFDLIKAG- 263
           + + VFY E+SPWW+ + + G PGH +R  ++TA E L K + ++  FR  ++  +++  
Sbjct: 182 DAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNP 241

Query: 264 -LKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTTNPESLERRIAEEW 323
            LK EG V SVN+  L+ G+        N+ P+   A FD R+ P  + ++ E ++    
Sbjct: 242 HLK-EGSVTSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEEQLQSWC 301

Query: 324 APASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPAST 383
             A   +TFEF Q      KF +P +T  D ++PWW     A +     L  PEIFPA+T
Sbjct: 302 QEAGEGVTFEFAQ------KFTEPRMTPTDDTDPWWAAFSGACKEMTLTL-EPEIFPAAT 361

Query: 384 DARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 435
           D+RY R +G+PA+GFSPM  TP+LLHDHNE L++A +L+G+++Y  ++ A AS
Sbjct: 362 DSRYIRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

BLAST of Pay0014469 vs. ExPASy Swiss-Prot
Match: Q03154 (Aminoacylase-1 OS=Homo sapiens OX=9606 GN=ACY1 PE=1 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 9.6e-96
Identity = 175/413 (42.37%), Postives = 247/413 (59.81%), Query Frame = 0

Query: 24  SEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFIISQAKSLSLESHTIEFVEGKPLI 83
           + + P      V+ F+QYL+I TVQP P Y  A  F    A+ L L    +E   G  + 
Sbjct: 2   TSKGPEEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVT 61

Query: 84  ILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQY 143
           +L WPG+N  L SILLNSHTDVVP     W+H P  A  DS G IYARG+QDMKCV +QY
Sbjct: 62  VLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCVSIQY 121

Query: 144 LEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSPG 203
           LEA+RRLK  G +  R+++++FVPDEE+GGH G E F +  EF  L     LDEG+ +P 
Sbjct: 122 LEAVRRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPT 181

Query: 204 ENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETVRRFRASQFDLIKAG- 263
           + + VFY E+SPWW+ + + G PGH +R  ++TA E L K + ++  FR  ++  +++  
Sbjct: 182 DAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNP 241

Query: 264 -LKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTTNPESLERRIAEEW 323
            LK EG V SVN+  L+ G+        N+ P+   A FD R+ P  + ++ E ++    
Sbjct: 242 HLK-EGSVTSVNLTKLEGGV------AYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWC 301

Query: 324 APASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPAST 383
             A   +T EF Q      K+  P +T  D SNPWW       ++ N  L  PEI PA+T
Sbjct: 302 QAAGEGVTLEFAQ------KWMHPQVTPTDDSNPWWAAFSRVCKDMNLTL-EPEIMPAAT 361

Query: 384 DARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 435
           D RY R +G+PA+GFSPM  TP+LLHDH+E L++A +L+G+++Y  ++ A AS
Sbjct: 362 DNRYIRAVGVPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALAS 400

BLAST of Pay0014469 vs. ExPASy TrEMBL
Match: A0A5A7TXA6 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G00610 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 5.2e-262
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360
           DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 449
           GIEVYESIIKAYASYDGDKRMESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of Pay0014469 vs. ExPASy TrEMBL
Match: A0A5D3BSI0 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold264G00590 PE=3 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 2.6e-261
Identity = 446/448 (99.55%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQ+PSSPSAI+SRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360
           DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 449
           GIEVYESIIKAYASYDGDKRMESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of Pay0014469 vs. ExPASy TrEMBL
Match: A0A1S3BJ13 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo OX=3656 GN=LOC103490443 PE=3 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 2.6e-261
Identity = 446/448 (99.55%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQ+PSSPSAI+SRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360
           DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 449
           GIEVYESIIKAYASYDGDKRMESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of Pay0014469 vs. ExPASy TrEMBL
Match: A0A0A0KAV8 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G117740 PE=3 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 4.3e-256
Identity = 439/448 (97.99%), Postives = 444/448 (99.11%), Query Frame = 0

Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAI+SRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPL+ILKWPGSN +LPSILLNSHTDVVPAEH KWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360
           DVR+PPTTNPESLERRIAEEWAPASRNMTFEFKQKESIY+KFGKPALTAIDKSNP WNLL
Sbjct: 301 DVRVPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPRWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 449
           GIEVYESIIKAYASYDGDK MESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 448

BLAST of Pay0014469 vs. ExPASy TrEMBL
Match: A0A6J1G695 (N-acyl-L-amino-acid amidohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111451263 PE=3 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 9.5e-232
Identity = 397/449 (88.42%), Postives = 419/449 (93.32%), Query Frame = 0

Query: 1   MNFKSLH-FLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADF 60
           MNFKSL+  LLSI TLF FLSIST+E+DP S S+IVSRFQ+YLQINTVQP+PQYYEAADF
Sbjct: 1   MNFKSLNLLLLSITTLFLFLSISTAEEDPLSSSSIVSRFQRYLQINTVQPNPQYYEAADF 60

Query: 61  IISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLG 120
           +ISQAKSLSLE HT+EFV GKPL++LKWPGSN +LPSILLNSHTDVVP EHHKWTHPPLG
Sbjct: 61  LISQAKSLSLEYHTVEFVVGKPLVLLKWPGSNPNLPSILLNSHTDVVPFEHHKWTHPPLG 120

Query: 121 AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEK 180
           AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGF+P+RSVYLSFVPDEEIGGHGGAEK
Sbjct: 121 AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFEPVRSVYLSFVPDEEIGGHGGAEK 180

Query: 181 FAESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALE 240
           FAESDEFKKLNVAIVLDEGLPSPGENYR FYGE+SPWWLVIKAVGAPGHGA+LYDN ALE
Sbjct: 181 FAESDEFKKLNVAIVLDEGLPSPGENYRAFYGERSPWWLVIKAVGAPGHGAKLYDNAALE 240

Query: 241 NLFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAG 300
           NLFKSIE+VRRFRASQFDL+KAGLK EG+VVSVNMV LK+G PSPTGFVMNLQPSEAEAG
Sbjct: 241 NLFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVSLKAGTPSPTGFVMNLQPSEAEAG 300

Query: 301 FDVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNL 360
           FDVRLPPT +PESLERRI EEWAP SRNMTFEFKQKESIYDKFGKPALTAID SNPWWNL
Sbjct: 301 FDVRLPPTADPESLERRITEEWAPVSRNMTFEFKQKESIYDKFGKPALTAIDSSNPWWNL 360

Query: 361 LEEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYL 420
           LEEAV NANGKL NPEIFPASTDARYFRN GLPAIGFSPM NTP LLHDHNEFLNQAEYL
Sbjct: 361 LEEAVSNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPSLLHDHNEFLNQAEYL 420

Query: 421 KGIEVYESIIKAYASYDGDKRMESFKDEL 449
           KGIEVYESIIK YASY G    ++FKDEL
Sbjct: 421 KGIEVYESIIKFYASYVG---RDNFKDEL 446

BLAST of Pay0014469 vs. NCBI nr
Match: KAA0047810.1 (aminoacylase-1 [Cucumis melo var. makuwa])

HSP 1 Score: 912.9 bits (2358), Expect = 1.1e-261
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360
           DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 449
           GIEVYESIIKAYASYDGDKRMESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of Pay0014469 vs. NCBI nr
Match: XP_008448158.1 (PREDICTED: aminoacylase-1 [Cucumis melo] >TYK01049.1 aminoacylase-1 [Cucumis melo var. makuwa])

HSP 1 Score: 910.6 bits (2352), Expect = 5.3e-261
Identity = 446/448 (99.55%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQ+PSSPSAI+SRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360
           DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 449
           GIEVYESIIKAYASYDGDKRMESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of Pay0014469 vs. NCBI nr
Match: XP_004139978.1 (aminoacylase-1 [Cucumis sativus] >KGN46643.1 hypothetical protein Csa_005514 [Cucumis sativus])

HSP 1 Score: 893.3 bits (2307), Expect = 8.8e-256
Identity = 439/448 (97.99%), Postives = 444/448 (99.11%), Query Frame = 0

Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAI+SRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPL+ILKWPGSN +LPSILLNSHTDVVPAEH KWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360
           DVR+PPTTNPESLERRIAEEWAPASRNMTFEFKQKESIY+KFGKPALTAIDKSNP WNLL
Sbjct: 301 DVRVPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPRWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 449
           GIEVYESIIKAYASYDGDK MESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 448

BLAST of Pay0014469 vs. NCBI nr
Match: XP_038876663.1 (aminoacylase-1 [Benincasa hispida])

HSP 1 Score: 864.8 bits (2233), Expect = 3.3e-247
Identity = 421/448 (93.97%), Postives = 433/448 (96.65%), Query Frame = 0

Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60
           M FKSLHFLLS ITLF  LS+STSE+DPSS SAI+SRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MIFKSLHFLLSFITLFLLLSVSTSEEDPSSTSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAK+LSLES TIEFVEGKPLI+LKWPG N DLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKALSLESQTIEFVEGKPLILLKWPGLNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGF+PLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFEPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESD F KLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGA+LYDN ALEN
Sbjct: 181 AESDVFNKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNAALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIE++RRFRASQFDLIKAGLKTEGDVVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIESIRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360
           DVRLPPT NPESLERRIAEEWAPASRNMTFEFKQKES+Y+KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESVYNKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 449
           GIEVY+SIIKAYASYDGDK  ESFKDEL
Sbjct: 421 GIEVYKSIIKAYASYDGDKPTESFKDEL 448

BLAST of Pay0014469 vs. NCBI nr
Match: XP_023532856.1 (aminoacylase-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 818.9 bits (2114), Expect = 2.1e-233
Identity = 400/449 (89.09%), Postives = 421/449 (93.76%), Query Frame = 0

Query: 1   MNFKSL-HFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADF 60
           MNFKSL H LLSI TLF FLSIS +E+DP S S+IVSRFQ+YLQINTVQP+PQYYEAADF
Sbjct: 1   MNFKSLNHLLLSITTLFLFLSISAAEEDPLSSSSIVSRFQRYLQINTVQPNPQYYEAADF 60

Query: 61  IISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLG 120
           IISQAKSLSLE HT+EFV GKPL++LKWPGSN +LPSILLNSHTDVVP EHHKWTHPPLG
Sbjct: 61  IISQAKSLSLEYHTVEFVVGKPLVLLKWPGSNPNLPSILLNSHTDVVPFEHHKWTHPPLG 120

Query: 121 AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEK 180
           AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGF+P+RSVYLSFVPDEEIGGHGGAEK
Sbjct: 121 AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFEPVRSVYLSFVPDEEIGGHGGAEK 180

Query: 181 FAESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALE 240
           FAESDEFKKLNVAIVLDEGLPSPGENYR FYGE+SPWWLVIKAVGAPGHGA+LYDN ALE
Sbjct: 181 FAESDEFKKLNVAIVLDEGLPSPGENYRAFYGERSPWWLVIKAVGAPGHGAKLYDNAALE 240

Query: 241 NLFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAG 300
           NLFKSIE+VRRFRASQFDL+KAGLK EG+VVSVNMV LK+G PSPTGFVMNLQPSEAEAG
Sbjct: 241 NLFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVSLKAGTPSPTGFVMNLQPSEAEAG 300

Query: 301 FDVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNL 360
           FDVRLPPT +PESLERRIAEEWAP SRNMTFEFKQKESIYDKFGKPALTAID SNPWWNL
Sbjct: 301 FDVRLPPTADPESLERRIAEEWAPVSRNMTFEFKQKESIYDKFGKPALTAIDSSNPWWNL 360

Query: 361 LEEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYL 420
           LEEAVRNANGKL NPEIFPASTDARYFRN GLPAIGFSPM NTP LLHDHNEFLNQAEYL
Sbjct: 361 LEEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPSLLHDHNEFLNQAEYL 420

Query: 421 KGIEVYESIIKAYASYDGDKRMESFKDEL 449
           KGIEVYESIIK+YASY G    ++FKDEL
Sbjct: 421 KGIEVYESIIKSYASYVG---RDNFKDEL 446

BLAST of Pay0014469 vs. TAIR 10
Match: AT4G38220.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 647.5 bits (1669), Expect = 7.8e-186
Identity = 315/428 (73.60%), Postives = 361/428 (84.35%), Query Frame = 0

Query: 9   LLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFIISQAKSLS 68
           LL ++ +   L +S    D     AIVSRFQ+YL+INTVQP+P+YY+A DFIISQAK LS
Sbjct: 3   LLRLLLVVVVLHLSAVAGD----DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLS 62

Query: 69  LESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNI 128
           LES TIEFV+GKPL++LKW GS+  LP+ LLNSHTDVVP E  KWTH PL AH+D HG+I
Sbjct: 63  LESQTIEFVKGKPLLLLKWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDI 122

Query: 129 YARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKK 188
           YARGSQDMKCVGMQYLEAIR+L+ASGF+PLRSVYLSFVPDEEIGGH GAEKFAES  FK 
Sbjct: 123 YARGSQDMKCVGMQYLEAIRKLQASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKS 182

Query: 189 LNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETV 248
           LN+AIVLDEGLPSP E+YRVFYGE+SPWWLVIKA G PGHGA+LYDN+A+ENL KSIE++
Sbjct: 183 LNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESI 242

Query: 249 RRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTT 308
           RRFRASQFDL+KAG   EGDVVSVNM FLK+G PSPTGFVMNLQPSEAEAGFD+R+PP+ 
Sbjct: 243 RRFRASQFDLLKAGGIAEGDVVSVNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSV 302

Query: 309 NPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLLEEAVRNAN 368
           + E+LERR+ EEWAPA+RNM+FEFKQK +     GK  LTA D SNPWW LLE AV+ A 
Sbjct: 303 DAEALERRLVEEWAPAARNMSFEFKQKLT-----GKQFLTAADDSNPWWGLLENAVKEAG 362

Query: 369 GKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESI 428
           G+ + PEIFPASTDARYFR  G+PA GFSP+ NTP LLHDHNE+L +AEYLKGIEVY SI
Sbjct: 363 GRTSKPEIFPASTDARYFRKAGVPAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSI 421

Query: 429 IKAYASYD 437
           IKAYASY+
Sbjct: 423 IKAYASYE 421

BLAST of Pay0014469 vs. TAIR 10
Match: AT4G38220.2 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 642.1 bits (1655), Expect = 3.3e-184
Identity = 315/431 (73.09%), Postives = 361/431 (83.76%), Query Frame = 0

Query: 9   LLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFIISQAKSLS 68
           LL ++ +   L +S    D     AIVSRFQ+YL+INTVQP+P+YY+A DFIISQAK LS
Sbjct: 3   LLRLLLVVVVLHLSAVAGD----DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLS 62

Query: 69  LESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNI 128
           LES TIEFV+GKPL++LKW GS+  LP+ LLNSHTDVVP E  KWTH PL AH+D HG+I
Sbjct: 63  LESQTIEFVKGKPLLLLKWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDI 122

Query: 129 YARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKK 188
           YARGSQDMKCVGMQYLEAIR+L+ASGF+PLRSVYLSFVPDEEIGGH GAEKFAES  FK 
Sbjct: 123 YARGSQDMKCVGMQYLEAIRKLQASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKS 182

Query: 189 LNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETV 248
           LN+AIVLDEGLPSP E+YRVFYGE+SPWWLVIKA G PGHGA+LYDN+A+ENL KSIE++
Sbjct: 183 LNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESI 242

Query: 249 RRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTT 308
           RRFRASQFDL+KAG   EGDVVSVNM FLK+G PSPTGFVMNLQPSEAEAGFD+R+PP+ 
Sbjct: 243 RRFRASQFDLLKAGGIAEGDVVSVNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSV 302

Query: 309 NPESLERRIAEEWAPASRNMTFE---FKQKESIYDKFGKPALTAIDKSNPWWNLLEEAVR 368
           + E+LERR+ EEWAPA+RNM+FE   FKQK +     GK  LTA D SNPWW LLE AV+
Sbjct: 303 DAEALERRLVEEWAPAARNMSFELGQFKQKLT-----GKQFLTAADDSNPWWGLLENAVK 362

Query: 369 NANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVY 428
            A G+ + PEIFPASTDARYFR  G+PA GFSP+ NTP LLHDHNE+L +AEYLKGIEVY
Sbjct: 363 EAGGRTSKPEIFPASTDARYFRKAGVPAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVY 422

Query: 429 ESIIKAYASYD 437
            SIIKAYASY+
Sbjct: 423 VSIIKAYASYE 424

BLAST of Pay0014469 vs. TAIR 10
Match: AT1G44820.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 528.1 bits (1359), Expect = 6.9e-150
Identity = 239/438 (54.57%), Postives = 323/438 (73.74%), Query Frame = 0

Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60
           M    L + L I ++ F L  S+SE+D       ++RFQQYL+ NT  P+P Y     F+
Sbjct: 1   MAISPLLWTLIIFSIIFSLQSSSSEEDTP-----ITRFQQYLRFNTAHPNPNYTAPISFL 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           I+QA+S+ L + TIEF+ GKP++++ W GSN +LPSIL NSH D VPAE  KWT+PP  A
Sbjct: 61  INQAQSIGLTTKTIEFISGKPILLITWLGSNPNLPSILFNSHLDSVPAESEKWTYPPFSA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           H    G+IYARG+QD KC+G+QYLE+IR LK+ GF PLR++++S+VP+EEIGG  G  KF
Sbjct: 121 HKTIDGHIYARGAQDDKCIGVQYLESIRNLKSRGFSPLRTIHISYVPEEEIGGFDGMMKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           A S EFK LN+   +DEG  +PG+ +RVFY ++ PW  VIKA G PGHGA+LYDN+A+EN
Sbjct: 181 AASSEFKDLNLGFAMDEGQANPGDEFRVFYADRVPWHFVIKAEGIPGHGAKLYDNSAMEN 240

Query: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           L KS+E + RFR SQFD +KAG     +V+SVN V+LK+G P+ TGFVMN+QPSEAEAG+
Sbjct: 241 LMKSVELISRFRESQFDFVKAGKAAYSEVISVNPVYLKAGTPTTTGFVMNMQPSEAEAGY 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360
           D+RLPP  +P+ +++RIAEEWAP+ RNMT+  ++K  + D  G+P +T ++ SNPWW++ 
Sbjct: 301 DLRLPPMADPDVMKKRIAEEWAPSIRNMTYSIQEKGKLRDHLGRPIMTPVNDSNPWWSIF 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           ++AV    GKLA PEI  ++TD+R+ R LG+P  GFSPM NTPILLHDHNEFL    ++K
Sbjct: 361 KQAVEAMGGKLAKPEILASTTDSRFIRTLGIPTFGFSPMTNTPILLHDHNEFLKDTVFMK 420

Query: 421 GIEVYESIIKAYASYDGD 439
           GIEVYES+I A +S++G+
Sbjct: 421 GIEVYESVISALSSFEGE 433

BLAST of Pay0014469 vs. TAIR 10
Match: AT1G44180.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 524.6 bits (1350), Expect = 7.6e-149
Identity = 238/431 (55.22%), Postives = 318/431 (73.78%), Query Frame = 0

Query: 6   LHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFIISQAK 65
           L + L + +  F L   + E++  +P   ++RFQQYL+ NT  P+P Y     F++ QA+
Sbjct: 6   LLWTLFLFSFIFSLQSHSKEEEEDTP---ITRFQQYLRFNTAHPNPNYTAPISFLLDQAR 65

Query: 66  SLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSH 125
           S+ L S TIEFV GKP+++L W GSNL+L SIL NSH D VPAE  KW HPP  AH    
Sbjct: 66  SIGLTSRTIEFVPGKPVLLLTWLGSNLNLSSILFNSHLDSVPAESEKWIHPPFSAHRTID 125

Query: 126 GNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDE 185
           G+IYARG+QD KC+G+QYLEAIR LK+  F PLR++++S+VP+EEIGG GG  KFA S E
Sbjct: 126 GHIYARGAQDDKCIGVQYLEAIRNLKSRSFSPLRTIHISYVPEEEIGGFGGMMKFAASSE 185

Query: 186 FKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSI 245
           FK LN+  V+DEG  SPG+ +RVFY E++PW L I+A G PGHGA+LYDN+A+ENL KS+
Sbjct: 186 FKDLNLGFVMDEGQASPGDEFRVFYAERTPWHLAIRANGMPGHGAKLYDNSAMENLMKSV 245

Query: 246 ETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLP 305
           E + RFR SQFD +KAG     +V+SVN V+LK+G PS TGFVMN+QPSEAE G+D+RLP
Sbjct: 246 ELISRFRESQFDFVKAGKAANSEVISVNPVYLKAGTPSTTGFVMNMQPSEAEVGYDLRLP 305

Query: 306 PTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLLEEAVR 365
           P  +P +L++RIAEEWAP+ RNMT+  KQ+  + D  G+P +T  + +NPWW++ ++AV 
Sbjct: 306 PMADPVALKKRIAEEWAPSIRNMTYTLKQQGKLTDHLGRPIMTTTNDTNPWWSIFKQAVE 365

Query: 366 NANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVY 425
              GKLA PEI  ++TD+R+ R LG+P +GFSPM+NTPIL+HDHNEFL    ++KGI VY
Sbjct: 366 ATGGKLAKPEILISTTDSRFIRTLGIPVLGFSPMINTPILVHDHNEFLKDTVFMKGIGVY 425

Query: 426 ESIIKAYASYD 437
           ES+I A +S++
Sbjct: 426 ESVISALSSFE 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q99JW21.3e-10045.26Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1[more]
Q6AYS73.5e-9844.25Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1[more]
Q55DP81.3e-9744.78Aminoacylase-1 OS=Dictyostelium discoideum OX=44689 GN=acy1 PE=2 SV=1[more]
Q6PTT03.0e-9742.62Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1[more]
Q031549.6e-9642.37Aminoacylase-1 OS=Homo sapiens OX=9606 GN=ACY1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TXA65.2e-262100.00N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A5D3BSI02.6e-26199.55N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BJ132.6e-26199.55N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo OX=3656 GN=LOC103490443 PE=3 ... [more]
A0A0A0KAV84.3e-25697.99N-acyl-L-amino-acid amidohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G117740 PE... [more]
A0A6J1G6959.5e-23288.42N-acyl-L-amino-acid amidohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111451263... [more]
Match NameE-valueIdentityDescription
KAA0047810.11.1e-261100.00aminoacylase-1 [Cucumis melo var. makuwa][more]
XP_008448158.15.3e-26199.55PREDICTED: aminoacylase-1 [Cucumis melo] >TYK01049.1 aminoacylase-1 [Cucumis mel... [more]
XP_004139978.18.8e-25697.99aminoacylase-1 [Cucumis sativus] >KGN46643.1 hypothetical protein Csa_005514 [Cu... [more]
XP_038876663.13.3e-24793.97aminoacylase-1 [Benincasa hispida][more]
XP_023532856.12.1e-23389.09aminoacylase-1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G38220.17.8e-18673.60Peptidase M20/M25/M40 family protein [more]
AT4G38220.23.3e-18473.09Peptidase M20/M25/M40 family protein [more]
AT1G44820.16.9e-15054.57Peptidase M20/M25/M40 family protein [more]
AT1G44180.17.6e-14955.22Peptidase M20/M25/M40 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.360coord: 217..327
e-value: 5.1E-8
score: 34.7
NoneNo IPR availablePIRSFPIRSF036696ACY-1coord: 23..437
e-value: 6.6E-175
score: 579.3
NoneNo IPR availableGENE3D1.10.150.900coord: 354..434
e-value: 1.7E-30
score: 106.6
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 27..216
e-value: 2.5E-58
score: 198.8
NoneNo IPR availablePANTHERPTHR45892AMINOACYLASE-1coord: 19..438
NoneNo IPR availablePANTHERPTHR45892:SF4BNAA06G40880D PROTEINcoord: 19..438
NoneNo IPR availableCDDcd05646M20_AcylaseI_likecoord: 35..433
e-value: 0.0
score: 661.275
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 32..433
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 98..430
e-value: 1.8E-37
score: 129.1
IPR010159N-acyl-L-amino-acid amidohydrolaseTIGRFAMTIGR01880TIGR01880coord: 32..434
e-value: 1.4E-186
score: 618.0
IPR001261ArgE/DapE/ACY1/CPG2/YscS, conserved sitePROSITEPS00758ARGE_DAPE_CPG2_1coord: 97..106
IPR001261ArgE/DapE/ACY1/CPG2/YscS, conserved sitePROSITEPS00759ARGE_DAPE_CPG2_2coord: 133..172

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0014469.1Pay0014469.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004046 aminoacylase activity
molecular_function GO:0016787 hydrolase activity