Pay0014400 (gene) Melon (Payzawat) v1

Overview
NamePay0014400
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionLipoyl synthase, mitochondrial
Locationchr01: 2660275 .. 2662463 (-)
RNA-Seq ExpressionPay0014400
SyntenyPay0014400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAAACGTTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCATTCTCTTCTTCATCTTCTTCCACCTCCGCCTCTGCCACATCTTCCCAATTCCCGCGCACGTTGGCCGGTCTCCGGGAACGACTCGCAGCGGAGTCCCCATCACTTTCCGACTTCGTTGATCTGCAATCTTCTGATTCGTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCTGGAGGCGAGAAATATGTTCAAATCAAGAAGAAGCTCCGGGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATTTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATTCTTGGTGACACTTGTACCCGTGGTTGCAGGTTAGTAGTAATTCAGTTTATTCAATCCATTTCTTTATGCTTATTTAATTGCCAATTAACGACTGAAGAACCATAATTTCTTGTTATTCTCCTGTGTGTAATGTCAATGTATATATAATTTAGTGGGGAACGTGAATGGATTTAGAACTGATTGTTTTTGTCATAGAAAATGACCCTGTTGATCACTTTGATATCATCGCGGTGCCTCAGAATTTCTAATTCCATTGCTGTGTGGGATTTTTTGAACACCAGGTTCTGTAACGTGAAGACATCACGAACTCCACCTCCACCGGATCCAAATGAGCCGACAAATGTTGCTGAAGCAATTGCATCATGGGGTTTGGATTATGTGGTCATTACTAGTGTCGATCGCGACGATTTACCCGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTGATCGAGGCATTGGGTACTAATTCCCTAGTTTCTATTACGCATAACAGTGTCTAGTTCATTGTTGCATTCAAGGATCCTTGATTTAAGGAACCTTATTTTATTTGGTTTCGGTGCAAATTTAGTGAAAGCTACTAATTTTACCCTGCACTGTTGATGTCGCCAAATCAGCATATGATATGGATGTGCTTCTGACTATGTTTTTCAGTGTGATTTAATCTCTTCTTTCATGTTGCTTATGCTTAACTAATTGATTTTGCAGTTCCTGATTTTCGAGGAGACTCTGGCTGTGTGGAGAAAGTTGCAAGGTCAGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAAGGAGCAGTTAGGGATCACCGTGCTAATTTCAAGCAATCATTAGACGTTCTAGTTATGGCCAAAGAGTTTTCTCCTTCTGGAACTCTTACAAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTTGTGCAAACAATGGAGAAGGTAAGAGCAGCTGGAGTTGATGTGATGACATTTGGGCAGTATATGAGACCTTCAAAACGCCATATGCCTGTATCAGAATACATTACTCCTGAGGCTTTTGAGAAGTATCAAAATCTTGGCATGAAAATGGTACTCTCTCTTTCTAATTATTTGAACAATTTGATTGTGTACTGTCTCCTCTGTTCTGCTAGTGAAAAATGATGATCGCTCAATCAATGCCTTAAAGGCTAAAGTCAACTTGATCTTTTTTTAAAAAAATTATAATATTGGGAATATTGTATCATTTCCCATAAGACTAGGATGAATCAAAGTGCATATCTTTTTCTCTAACCTAAATATCTATTTTAAGCACACTTTAAAAGTTAATCCAAACAGGTCTTCTTGGATTTGTACAGTCTGAATAACTTTCATTTGCATTTTCCTTCTCTTGGATCAGTATCGTTGTAATTTTATGTGTGTTTGATTTGTTAGAGGGAGTCCATGTTTTACTTTAAAACATATACTAATTCCCACTTTGATTCATAACCCTCACTTTTAAGTTTATAGGAAATCTCCATGATCAAGTATTACTTATTATATGATCAATTGTTTTTTGATGGCTGGAGTTGCCTGCCATGCCTTGAGGGTGCAACAATTATCTAAAAAATCCATTTTTTTTTGTTTGGTTGTGGCAGGGTTTTCGATATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACAGCCTACCCAACTTTGA

mRNA sequence

ATGAACAAACGTTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCATTCTCTTCTTCATCTTCTTCCACCTCCGCCTCTGCCACATCTTCCCAATTCCCGCGCACGTTGGCCGGTCTCCGGGAACGACTCGCAGCGGAGTCCCCATCACTTTCCGACTTCGTTGATCTGCAATCTTCTGATTCGTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCTGGAGGCGAGAAATATGTTCAAATCAAGAAGAAGCTCCGGGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATTTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATTCTTGGTGACACTTGTACCCGTGGTTGCAGGTTCTGTAACGTGAAGACATCACGAACTCCACCTCCACCGGATCCAAATGAGCCGACAAATGTTGCTGAAGCAATTGCATCATGGGGTTTGGATTATGTGGTCATTACTAGTGTCGATCGCGACGATTTACCCGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTGATCGAGGCATTGGTTCCTGATTTTCGAGGAGACTCTGGCTGTGTGGAGAAAGTTGCAAGGTCAGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAAGGAGCAGTTAGGGATCACCGTGCTAATTTCAAGCAATCATTAGACGTTCTAGTTATGGCCAAAGAGTTTTCTCCTTCTGGAACTCTTACAAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTTGTGCAAACAATGGAGAAGGTAAGAGCAGCTGGAGTTGATGTGATGACATTTGGGCAGTATATGAGACCTTCAAAACGCCATATGCCTGTATCAGAATACATTACTCCTGAGGCTTTTGAGAAGTATCAAAATCTTGGCATGAAAATGGGTTTTCGATATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACAGCCTACCCAACTTTGA

Coding sequence (CDS)

ATGAACAAACGTTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCATTCTCTTCTTCATCTTCTTCCACCTCCGCCTCTGCCACATCTTCCCAATTCCCGCGCACGTTGGCCGGTCTCCGGGAACGACTCGCAGCGGAGTCCCCATCACTTTCCGACTTCGTTGATCTGCAATCTTCTGATTCGTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCTGGAGGCGAGAAATATGTTCAAATCAAGAAGAAGCTCCGGGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATTTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATTCTTGGTGACACTTGTACCCGTGGTTGCAGGTTCTGTAACGTGAAGACATCACGAACTCCACCTCCACCGGATCCAAATGAGCCGACAAATGTTGCTGAAGCAATTGCATCATGGGGTTTGGATTATGTGGTCATTACTAGTGTCGATCGCGACGATTTACCCGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTGATCGAGGCATTGGTTCCTGATTTTCGAGGAGACTCTGGCTGTGTGGAGAAAGTTGCAAGGTCAGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAAGGAGCAGTTAGGGATCACCGTGCTAATTTCAAGCAATCATTAGACGTTCTAGTTATGGCCAAAGAGTTTTCTCCTTCTGGAACTCTTACAAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTTGTGCAAACAATGGAGAAGGTAAGAGCAGCTGGAGTTGATGTGATGACATTTGGGCAGTATATGAGACCTTCAAAACGCCATATGCCTGTATCAGAATACATTACTCCTGAGGCTTTTGAGAAGTATCAAAATCTTGGCATGAAAATGGGTTTTCGATATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACAGCCTACCCAACTTTGA

Protein sequence

MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASNSQPTQL
Homology
BLAST of Pay0014400 vs. ExPASy Swiss-Prot
Match: Q3LSN5 (Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 2.0e-182
Identity = 316/375 (84.27%), Postives = 349/375 (93.07%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M  R +++A  L   ++  SSSS++T+ ++  S+  + LA LR RLA ESPSLSDF+ L+
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S ++YSVEVGTKKKPLPKPKWMKES+PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHF ETVQKLK LKP+MLIEALVPDFRG++ CVEKV++SGLDVFAHNI
Sbjct: 182 SVDRDDLPDQGSGHFTETVQKLKALKPSMLIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ AVRDHRANFKQSLDVL+MAKE++P+GTLTKTSIMLGCGETPDQ+V+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQIVKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQ+MRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQHMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASN 376
           FYIKSMI+SDRAAS+
Sbjct: 362 FYIKSMIDSDRAASS 376

BLAST of Pay0014400 vs. ExPASy Swiss-Prot
Match: Q3LSN4 (Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 2.5e-182
Identity = 316/375 (84.27%), Postives = 348/375 (92.80%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M  R +++A  L   ++  SSSS++T+ ++  S+  + LA LR RLA ESPSLSDF+ L+
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S ++YSVEVGTKKKPLPKPKWMKES+PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHF ETVQKLK LKP+ LIEALVPDFRG++ CVEKV++SGLDVFAHNI
Sbjct: 182 SVDRDDLPDQGSGHFTETVQKLKALKPSTLIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ AVRDHRANFKQSLDVL+MAKE++P+GTLTKTSIMLGCGETPDQ+V+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQIVKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASN 376
           FYIKSMI+SDRAAS+
Sbjct: 362 FYIKSMIDSDRAASS 376

BLAST of Pay0014400 vs. ExPASy Swiss-Prot
Match: B9H5L9 (Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 4.3e-182
Identity = 316/366 (86.34%), Postives = 343/366 (93.72%), Query Frame = 0

Query: 13  KSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTK 72
           K+ +R+FSSS+   S++    QF +TLAGLR RLA ESP+LSDF+ LQS+++YSVEVGTK
Sbjct: 19  KAKNRTFSSSTVE-SSTKQPPQFSQTLAGLRARLAVESPTLSDFIHLQSNNTYSVEVGTK 78

Query: 73  KKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATI 132
           KKPLPKPKWM+E++PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATI
Sbjct: 79  KKPLPKPKWMREAIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATI 138

Query: 133 MILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGS 192
           MILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDL DQGS
Sbjct: 139 MILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLADQGS 198

Query: 193 GHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNIETVEELQGAVRD 252
           GHFAETV KLK LKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNIETVEELQ +VRD
Sbjct: 199 GHFAETVHKLKTLKPNMLIEALVPDFRGDRGCVEKVAKSGLDVFAHNIETVEELQSSVRD 258

Query: 253 HRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQY 312
           HRANFKQSLDVL+MAKE++P GTLTKTSIMLGCGE P+QVV+TMEKVRAAGVDVMTFGQY
Sbjct: 259 HRANFKQSLDVLMMAKEYAPPGTLTKTSIMLGCGEAPEQVVKTMEKVRAAGVDVMTFGQY 318

Query: 313 MRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRA 372
           MRPSKRHMPVSEYITP+AFEKY+ LGM+MGFRYVASGPMVRSSYKAGEFYIKSMIESDR+
Sbjct: 319 MRPSKRHMPVSEYITPDAFEKYKTLGMEMGFRYVASGPMVRSSYKAGEFYIKSMIESDRS 378

Query: 373 ASNSQP 379
            S+  P
Sbjct: 379 VSSQLP 383

BLAST of Pay0014400 vs. ExPASy Swiss-Prot
Match: B7FM45 (Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 2.4e-180
Identity = 310/375 (82.67%), Postives = 349/375 (93.07%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M  R +++A+ LKS ++ FS ++++T+ + +SS+FP+ L  LR RLA ESPSLSDF+ L+
Sbjct: 2   MYSRFRTVAKNLKSTTKPFSFTTATTTTTVSSSEFPQNLTELRARLARESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S+++YSVEVGTKK PLPKPKWMKES+PGG KYVQIKKKLRELKLHTVCEEAKCPN+GECW
Sbjct: 62  SNNAYSVEVGTKKNPLPKPKWMKESIPGGWKYVQIKKKLRELKLHTVCEEAKCPNMGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SV RDDLPDQGS HF ETVQKLK+LKP++LIEALVPDFRG++ CVEKV++SGLDVFAHNI
Sbjct: 182 SVGRDDLPDQGSSHFTETVQKLKILKPSILIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ AVRDHRANF QSLDVL MAK+++P+GTLTKTSIMLGCGETPDQ+V+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFNQSLDVLRMAKDYAPAGTLTKTSIMLGCGETPDQIVKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASN 376
           FYIKSMI+SDRA S+
Sbjct: 362 FYIKSMIDSDRAVSS 376

BLAST of Pay0014400 vs. ExPASy Swiss-Prot
Match: Q9ZWT1 (Lipoyl synthase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 3.2e-177
Identity = 311/376 (82.71%), Postives = 340/376 (90.43%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M+ R   L R L+  SR FSSSS+ T  + T S  P++L  LR RLA ESPSL+DF+   
Sbjct: 1   MHSRSALLYRFLRPASRCFSSSSAVTPVTVTQS--PKSLEALRARLANESPSLTDFI--- 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
             D+YSVEVGTKKKPLPKPKWMKES+PGGE+YVQIKKKLR+LKLHTVCEEAKCPNLGECW
Sbjct: 61  HGDTYSVEVGTKKKPLPKPKWMKESIPGGERYVQIKKKLRDLKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWG+DYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPNNVAEAIASWGVDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQ+LK LKP MLIEALVPDFRGD GCVEKV++SGLDV AHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQRLKFLKPEMLIEALVPDFRGDGGCVEKVSKSGLDVLAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRANFKQSLDVL MAKE++P+GTLTKTS+MLGCGETPDQVV+TMEKVR
Sbjct: 241 ETVEELQSFVRDHRANFKQSLDVLRMAKEYAPAGTLTKTSVMLGCGETPDQVVKTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPV+EY+TP+AFE+Y+ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVAEYVTPDAFERYRLLGMEMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNS 377
           +YIKSMIE+DR AS S
Sbjct: 361 YYIKSMIEADRVASPS 371

BLAST of Pay0014400 vs. ExPASy TrEMBL
Match: A0A5A7SKJ1 (Lipoyl synthase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.3e-213
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQL 382
           FYIKSMIESDRAASNSQPTQL
Sbjct: 361 FYIKSMIESDRAASNSQPTQL 381

BLAST of Pay0014400 vs. ExPASy TrEMBL
Match: A0A1S4E162 (Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.3e-213
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQL 382
           FYIKSMIESDRAASNSQPTQL
Sbjct: 361 FYIKSMIESDRAASNSQPTQL 381

BLAST of Pay0014400 vs. ExPASy TrEMBL
Match: A0A0A0K1R3 (Lipoyl synthase, mitochondrial OS=Cucumis sativus OX=3659 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 1.0e-210
Identity = 376/381 (98.69%), Postives = 378/381 (99.21%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESP LSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVLVMAKEFS SGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQL 382
           FYIKSMIESDRAASNSQPTQL
Sbjct: 361 FYIKSMIESDRAASNSQPTQL 381

BLAST of Pay0014400 vs. ExPASy TrEMBL
Match: A0A6J1D3N5 (Lipoyl synthase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016734 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 7.9e-203
Identity = 362/380 (95.26%), Postives = 367/380 (96.58%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M   CQSL RIL+S  RSFSSSSSSTSASATSSQFPRTLA LRERLAAESPSLSDFV LQ
Sbjct: 1   MKTPCQSLVRILESFGRSFSSSSSSTSASATSSQFPRTLAALRERLAAESPSLSDFVGLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S+DSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  STDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQ LKVLKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQTLKVLKPNMLIEALVPDFRGDIGCVEKVAKSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVL MAK+F+PSGTLTKTSIMLGCGE PDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLAMAKDFAPSGTLTKTSIMLGCGEKPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQ 381
           FYIKSMIESDRAASNSQPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

BLAST of Pay0014400 vs. ExPASy TrEMBL
Match: A0A6J1ERM2 (Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111437162 PE=3 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.2e-200
Identity = 360/380 (94.74%), Postives = 366/380 (96.32%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MN R  SLARI +SV RS S SSSSTSASATSSQ PRTLAGLRERLAAESP+LSDFV L+
Sbjct: 1   MNARRHSLARIFESVGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVGLK 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPQNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGD G VEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGSVEKVAKSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVLVMAKEF+PSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY NLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQ 381
           FYIKSMIESDRAAS+S PTQ
Sbjct: 361 FYIKSMIESDRAASSSHPTQ 380

BLAST of Pay0014400 vs. NCBI nr
Match: XP_016901725.1 (PREDICTED: lipoyl synthase 2, mitochondrial isoform X1 [Cucumis melo] >KAA0031592.1 lipoyl synthase 2 [Cucumis melo var. makuwa] >TYK07044.1 lipoyl synthase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 751.9 bits (1940), Expect = 2.7e-213
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQL 382
           FYIKSMIESDRAASNSQPTQL
Sbjct: 361 FYIKSMIESDRAASNSQPTQL 381

BLAST of Pay0014400 vs. NCBI nr
Match: XP_004136815.1 (lipoyl synthase 2, mitochondrial [Cucumis sativus] >KGN43590.1 hypothetical protein Csa_020197 [Cucumis sativus])

HSP 1 Score: 742.3 bits (1915), Expect = 2.1e-210
Identity = 376/381 (98.69%), Postives = 378/381 (99.21%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESP LSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVLVMAKEFS SGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQL 382
           FYIKSMIESDRAASNSQPTQL
Sbjct: 361 FYIKSMIESDRAASNSQPTQL 381

BLAST of Pay0014400 vs. NCBI nr
Match: XP_038887854.1 (lipoyl synthase 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 741.9 bits (1914), Expect = 2.8e-210
Identity = 374/380 (98.42%), Postives = 378/380 (99.47%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MNKRCQSL RILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ
Sbjct: 1   MNKRCQSLTRILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETV KLKVLKPNMLIEALVPDFRGD+GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVHKLKVLKPNMLIEALVPDFRGDTGCVEKVAKSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVLVMAKEF+PSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQ 381
           FYIKSMIESDRAASNSQPT+
Sbjct: 361 FYIKSMIESDRAASNSQPTR 380

BLAST of Pay0014400 vs. NCBI nr
Match: XP_022147922.1 (lipoyl synthase 2, mitochondrial [Momordica charantia])

HSP 1 Score: 716.1 bits (1847), Expect = 1.6e-202
Identity = 362/380 (95.26%), Postives = 367/380 (96.58%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M   CQSL RIL+S  RSFSSSSSSTSASATSSQFPRTLA LRERLAAESPSLSDFV LQ
Sbjct: 1   MKTPCQSLVRILESFGRSFSSSSSSTSASATSSQFPRTLAALRERLAAESPSLSDFVGLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S+DSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  STDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQ LKVLKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQTLKVLKPNMLIEALVPDFRGDIGCVEKVAKSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVL MAK+F+PSGTLTKTSIMLGCGE PDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLAMAKDFAPSGTLTKTSIMLGCGEKPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQ 381
           FYIKSMIESDRAASNSQPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

BLAST of Pay0014400 vs. NCBI nr
Match: XP_023531015.1 (lipoyl synthase 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 710.3 bits (1832), Expect = 9.0e-201
Identity = 361/380 (95.00%), Postives = 366/380 (96.32%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MN R  SLARI +SV RS S SSSSTSASATSSQ PRTLAGLRERLAAESP+LSDFV L+
Sbjct: 1   MNTRRHSLARIFESVGRSLSYSSSSTSASATSSQIPRTLAGLRERLAAESPTLSDFVGLK 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPQNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGD G VEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGSVEKVAKSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVLVMAKEF+PSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY NLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQ 381
           FYIKSMIESDRAASNS PTQ
Sbjct: 361 FYIKSMIESDRAASNSHPTQ 380

BLAST of Pay0014400 vs. TAIR 10
Match: AT2G20860.1 (lipoic acid synthase 1 )

HSP 1 Score: 622.5 bits (1604), Expect = 2.3e-178
Identity = 311/376 (82.71%), Postives = 340/376 (90.43%), Query Frame = 0

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M+ R   L R L+  SR FSSSS+ T  + T S  P++L  LR RLA ESPSL+DF+   
Sbjct: 1   MHSRSALLYRFLRPASRCFSSSSAVTPVTVTQS--PKSLEALRARLANESPSLTDFI--- 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
             D+YSVEVGTKKKPLPKPKWMKES+PGGE+YVQIKKKLR+LKLHTVCEEAKCPNLGECW
Sbjct: 61  HGDTYSVEVGTKKKPLPKPKWMKESIPGGERYVQIKKKLRDLKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWG+DYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPNNVAEAIASWGVDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQ+LK LKP MLIEALVPDFRGD GCVEKV++SGLDV AHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQRLKFLKPEMLIEALVPDFRGDGGCVEKVSKSGLDVLAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRANFKQSLDVL MAKE++P+GTLTKTS+MLGCGETPDQVV+TMEKVR
Sbjct: 241 ETVEELQSFVRDHRANFKQSLDVLRMAKEYAPAGTLTKTSVMLGCGETPDQVVKTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPV+EY+TP+AFE+Y+ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVAEYVTPDAFERYRLLGMEMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNS 377
           +YIKSMIE+DR AS S
Sbjct: 361 YYIKSMIEADRVASPS 371

BLAST of Pay0014400 vs. TAIR 10
Match: AT5G08415.1 (Radical SAM superfamily protein )

HSP 1 Score: 380.9 bits (977), Expect = 1.2e-105
Identity = 176/299 (58.86%), Postives = 231/299 (77.26%), Query Frame = 0

Query: 78  KPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGD 137
           KP W+++  P GE++ ++K+ L  L L+TVCEEA+CPN+GECW+GG  G ATATIM+LGD
Sbjct: 97  KPAWLRQKAPQGERFQEVKESLSRLNLNTVCEEAQCPNIGECWNGGGDGVATATIMVLGD 156

Query: 138 TCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAE 197
           TCTRGCRFC VKTSR PPPPDP EP N A+AIASWG+DY+VITSVDRDD+PD GSGHFA+
Sbjct: 157 TCTRGCRFCAVKTSRNPPPPDPMEPENTAKAIASWGVDYIVITSVDRDDIPDGGSGHFAQ 216

Query: 198 TVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNIETVEELQGAVRDHRANF 257
           TV+ +K  KP+++IE L  DFRGD   V+ +  SGLDVFAHN+ETV+ LQ  VRD RA +
Sbjct: 217 TVKAMKRHKPDIMIECLTSDFRGDLEAVDTLVHSGLDVFAHNVETVKRLQRLVRDPRAGY 276

Query: 258 KQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSK 317
           +QS+ VL  AK  S  G +TKTSIMLG GET +++ + M  +RA  VD++T GQY++P+ 
Sbjct: 277 EQSMSVLKHAK-ISKPGMITKTSIMLGLGETDEELKEAMADLRAIDVDILTLGQYLQPTP 336

Query: 318 RHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASNS 377
            H+ V EY+TPE F+ ++  G  +GFRYVASGP+VRSSY+AGE ++K+M++   + S S
Sbjct: 337 LHLTVKEYVTPEKFDFWKTYGESIGFRYVASGPLVRSSYRAGELFVKTMVKESYSKSLS 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3LSN52.0e-18284.27Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1[more]
Q3LSN42.5e-18284.27Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1[more]
B9H5L94.3e-18286.34Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1[more]
B7FM452.4e-18082.67Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1[more]
Q9ZWT13.2e-17782.71Lipoyl synthase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7SKJ11.3e-213100.00Lipoyl synthase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=LIP1 PE... [more]
A0A1S4E1621.3e-213100.00Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1[more]
A0A0A0K1R31.0e-21098.69Lipoyl synthase, mitochondrial OS=Cucumis sativus OX=3659 GN=LIP1 PE=3 SV=1[more]
A0A6J1D3N57.9e-20395.26Lipoyl synthase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016734 PE... [more]
A0A6J1ERM22.2e-20094.74Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111437162 PE=... [more]
Match NameE-valueIdentityDescription
XP_016901725.12.7e-213100.00PREDICTED: lipoyl synthase 2, mitochondrial isoform X1 [Cucumis melo] >KAA003159... [more]
XP_004136815.12.1e-21098.69lipoyl synthase 2, mitochondrial [Cucumis sativus] >KGN43590.1 hypothetical prot... [more]
XP_038887854.12.8e-21098.42lipoyl synthase 2, mitochondrial [Benincasa hispida][more]
XP_022147922.11.6e-20295.26lipoyl synthase 2, mitochondrial [Momordica charantia][more]
XP_023531015.19.0e-20195.00lipoyl synthase 2, mitochondrial [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G20860.12.3e-17882.71lipoic acid synthase 1 [more]
AT5G08415.11.2e-10558.86Radical SAM superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006638Elp3/MiaB/NifBSMARTSM00729MiaBcoord: 129..336
e-value: 2.6E-7
score: 40.3
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 122..349
e-value: 1.0E-13
score: 52.9
IPR003698Lipoyl synthaseTIGRFAMTIGR00510TIGR00510coord: 72..366
e-value: 1.3E-121
score: 403.5
IPR003698Lipoyl synthasePIRSFPIRSF005963Lipoyl_synthcoord: 15..380
e-value: 5.0E-147
score: 487.5
IPR003698Lipoyl synthaseSFLDSFLDF00271lipoyl_synthasecoord: 70..366
e-value: 0.0
score: 436.0
IPR003698Lipoyl synthasePANTHERPTHR10949LIPOYL SYNTHASEcoord: 34..375
IPR003698Lipoyl synthaseHAMAPMF_00206Lipoyl_synthcoord: 78..372
score: 132.858719
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 136..298
e-value: 5.4E-11
score: 43.2
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 124..344
score: 27.424414
IPR031691Lipoyl synthase, N-terminalPFAMPF16881LIAS_Ncoord: 5..113
e-value: 1.7E-17
score: 63.8
NoneNo IPR availableSFLDSFLDG01058lipoyl_synthase_likecoord: 70..366
e-value: 0.0
score: 436.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..36
NoneNo IPR availablePANTHERPTHR10949:SF0LIPOYL SYNTHASE, MITOCHONDRIALcoord: 34..375
NoneNo IPR availableCDDcd01335Radical_SAMcoord: 133..313
e-value: 3.00606E-7
score: 48.4838
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 94..369
IPR027527Lipoyl synthase, mitochondrialHAMAPMF_03128Lipoyl_synth_plantMcoord: 20..375
score: 62.162292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0014400.1Pay0014400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009107 lipoate biosynthetic process
biological_process GO:0009249 protein lipoylation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0016992 lipoate synthase activity
molecular_function GO:0102552 lipoyl synthase activity (acting on glycine-cleavage complex H protein
molecular_function GO:0102553 lipoyl synthase activity (acting on pyruvate dehydrogenase E2 protein)
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding