Pay0014328 (gene) Melon (Payzawat) v1

Overview
NamePay0014328
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionE3 ubiquitin-protein ligase RHA1B
Locationchr11: 30084017 .. 30084720 (-)
RNA-Seq ExpressionPay0014328
SyntenyPay0014328
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATCAAATTCCCCCCTCCTTCCCTAACACAGACTCCCCCATTAACTAACCTTTACTTCAATCTACAACATTCAACCAATTTTTCCACCTCCCTTATGGGCTTTCCGGTGGGTTGCACCGAAGTTTTCTTCCCCACCATATTCCTCCACCTCCTCTCCCTCCTCGACCTTCTCAAATTCCTCATCCTCTCCCTCTTCCGCCTCCTCGGCCTCCCCGAATTTCTCCCCTCCGACAATCTACCGCCTGAACTCCAATTCCCCCTCAACCCTCCCCCGTCGGCCCTCCTCCTCCGCGAACTCCTCCCGGTGGTGAAATTCTCCGATCTGCCAAACCCGCCAGAGAGATGCGCCGTTTGCCTGTACGAGTTCGAGGGGGCGGAGGAGATCCGGTGGCTAACGAATTGCAAGCACATATTCCACCGGAGGTGTCTGGACCGTTGGATGGATCATGATCACGACACGTGTCCTCTTTGTAGAACGCCGTTTGTGCCCGATGGGATGATGGATGAATTTAATCAACGGCTGTGGGCTGCCTCCGGTGTCGCCGAGACTTTCGAGACGGACTTTAACTCCGTTTTAGGATAGAACCTTTTTCTTTCTTTTGTTTTCCATTATTCTTTTTCTTCTTTTTCACTTTCTTTTTTTCTTTTTTTTTTAAGGAAGAGTTTTTCAAACTGTATATAATAATTATAAATCTATAATGTG

mRNA sequence

AAATCAAATTCCCCCCTCCTTCCCTAACACAGACTCCCCCATTAACTAACCTTTACTTCAATCTACAACATTCAACCAATTTTTCCACCTCCCTTATGGGCTTTCCGGTGGGTTGCACCGAAGTTTTCTTCCCCACCATATTCCTCCACCTCCTCTCCCTCCTCGACCTTCTCAAATTCCTCATCCTCTCCCTCTTCCGCCTCCTCGGCCTCCCCGAATTTCTCCCCTCCGACAATCTACCGCCTGAACTCCAATTCCCCCTCAACCCTCCCCCGTCGGCCCTCCTCCTCCGCGAACTCCTCCCGGTGGTGAAATTCTCCGATCTGCCAAACCCGCCAGAGAGATGCGCCGTTTGCCTGTACGAGTTCGAGGGGGCGGAGGAGATCCGGTGGCTAACGAATTGCAAGCACATATTCCACCGGAGGTGTCTGGACCGTTGGATGGATCATGATCACGACACGTGTCCTCTTTGTAGAACGCCGTTTGTGCCCGATGGGATGATGGATGAATTTAATCAACGGCTGTGGGCTGCCTCCGGTGTCGCCGAGACTTTCGAGACGGACTTTAACTCCGTTTTAGGATAGAACCTTTTTCTTTCTTTTGTTTTCCATTATTCTTTTTCTTCTTTTTCACTTTCTTTTTTTCTTTTTTTTTTAAGGAAGAGTTTTTCAAACTGTATATAATAATTATAAATCTATAATGTG

Coding sequence (CDS)

ATGGGCTTTCCGGTGGGTTGCACCGAAGTTTTCTTCCCCACCATATTCCTCCACCTCCTCTCCCTCCTCGACCTTCTCAAATTCCTCATCCTCTCCCTCTTCCGCCTCCTCGGCCTCCCCGAATTTCTCCCCTCCGACAATCTACCGCCTGAACTCCAATTCCCCCTCAACCCTCCCCCGTCGGCCCTCCTCCTCCGCGAACTCCTCCCGGTGGTGAAATTCTCCGATCTGCCAAACCCGCCAGAGAGATGCGCCGTTTGCCTGTACGAGTTCGAGGGGGCGGAGGAGATCCGGTGGCTAACGAATTGCAAGCACATATTCCACCGGAGGTGTCTGGACCGTTGGATGGATCATGATCACGACACGTGTCCTCTTTGTAGAACGCCGTTTGTGCCCGATGGGATGATGGATGAATTTAATCAACGGCTGTGGGCTGCCTCCGGTGTCGCCGAGACTTTCGAGACGGACTTTAACTCCGTTTTAGGATAG

Protein sequence

MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPPSALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG
Homology
BLAST of Pay0014328 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 1.7e-50
Identity = 98/159 (61.64%), Postives = 116/159 (72.96%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPE-LQFPLNPP 60
           MGFPVG TEVF P +F+  LS+L  ++ ++ S+FR LGL +FL  D   P+   +P   P
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYTSYPTRIP 60

Query: 61  P-----SALLLRELLPVVKFSDLPNP----PERCAVCLYEFEGAEEIRWLTNCKHIFHRR 120
                 SALL+RE+LPV+KF +L N     PE CAVCLYEFEG +EIRWL NC+HIFHR 
Sbjct: 61  ETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRS 120

Query: 121 CLDRWMDHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGV 150
           CLDRWMDHD  TCPLCRTPFVPD M +EFNQRLWAASGV
Sbjct: 121 CLDRWMDHDQKTCPLCRTPFVPDEMQEEFNQRLWAASGV 159

BLAST of Pay0014328 vs. ExPASy Swiss-Prot
Match: Q9SUS4 (Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RHA1A PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.2e-18
Identity = 57/151 (37.75%), Postives = 87/151 (57.62%), Query Frame = 0

Query: 1   MGFPVG-CTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSD--NLPPELQFPLN 60
           MG P    TE+  P+  L +L ++   + ++ +L   +GLP FL  +  + P   +  L+
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHNETSAPDLTRHALS 60

Query: 61  PPPSALLLRELLPVVKFSDLPNPPER-CAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRW- 120
              SA L  EL+PVV+FSDLP  PE  C VCL +FE  +++R L  C H+FH  CLDRW 
Sbjct: 61  --TSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYCLDRWI 120

Query: 121 MDHDHDTCPLCRTPFVPDGMMDEFNQRLWAA 147
           +D++   CP+CR  F+P    +++ Q  W +
Sbjct: 121 VDYNKMKCPVCRHRFLP---KEKYTQSDWGS 146

BLAST of Pay0014328 vs. ExPASy Swiss-Prot
Match: Q9SUS5 (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 3.8e-18
Identity = 57/149 (38.26%), Postives = 78/149 (52.35%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60
           MG P    E+  P   L  L ++   + ++ +L   +GLP FL  +         L    
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALST 60

Query: 61  SALLLRELLPVVKFSDLPNPPER-CAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRW-MDH 120
           SA L  EL+PVV+FSDL   PE  C VCL +F   ++IR L  C H+FH RCLDRW +D 
Sbjct: 61  SATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRWIVDC 120

Query: 121 DHDTCPLCRTPFVPDGMMDEFNQRLWAAS 148
           +  TCP+CR  F+P+     F+   W  S
Sbjct: 121 NKITCPICRNRFLPEEKSTPFD---WGTS 146

BLAST of Pay0014328 vs. ExPASy Swiss-Prot
Match: Q5EAE9 (RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 5.8e-11
Identity = 33/72 (45.83%), Postives = 41/72 (56.94%), Query Frame = 0

Query: 67  ELLPVVKFSDLPNPPE--RCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDHDTCP 126
           E LPV +F  L    +   CAVCL  FE  E +R L  CKH FH  C+D W+D  H TCP
Sbjct: 127 ESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHVECVDTWLD-AHSTCP 186

Query: 127 LCRTPFVPDGMM 137
           LCR    P+ ++
Sbjct: 187 LCRYRVDPEDIL 197

BLAST of Pay0014328 vs. ExPASy Swiss-Prot
Match: Q6NML0 (E3 ubiquitin-protein ligase ATL76 OS=Arabidopsis thaliana OX=3702 GN=ATL76 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.2e-10
Identity = 29/63 (46.03%), Postives = 39/63 (61.90%), Query Frame = 0

Query: 68  LLPVVKFS---DLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDHDTCP 127
           + PVV +S   +LP   E C +CL +F   E++R L  C H FH RC+D+W+ H H TCP
Sbjct: 116 MFPVVSYSREMNLPGIGEECVICLSDFVSGEQLRLLPKCNHGFHVRCIDKWLQH-HLTCP 175

BLAST of Pay0014328 vs. ExPASy TrEMBL
Match: A0A5D3BV35 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001290 PE=4 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 7.7e-91
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60
           MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120
           SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 163
           DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG
Sbjct: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 162

BLAST of Pay0014328 vs. ExPASy TrEMBL
Match: A0A1S3C782 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103497696 PE=4 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 7.7e-91
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60
           MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120
           SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 163
           DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG
Sbjct: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 162

BLAST of Pay0014328 vs. ExPASy TrEMBL
Match: A0A0A0K874 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G006705 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.1e-88
Identity = 157/162 (96.91%), Postives = 160/162 (98.77%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60
           MGFPVGCTEVFFPTIFLHLLSLLD+LKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDVLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120
           SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRC+D WMDHDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDSWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 163
           DTCPLCRTPFVPD MMDEFNQRLWAASG+AETFETDFNSVLG
Sbjct: 121 DTCPLCRTPFVPDEMMDEFNQRLWAASGIAETFETDFNSVLG 162

BLAST of Pay0014328 vs. ExPASy TrEMBL
Match: A0A6J1KF16 (probable E3 ubiquitin-protein ligase RHA1A OS=Cucurbita maxima OX=3661 GN=LOC111494424 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.6e-70
Identity = 137/164 (83.54%), Postives = 146/164 (89.02%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSD-NLPPELQFPLNPP 60
           MGFPVGCTEVFFPTIFLHLLSLLDLLK LIL LFRLLGLPEFL SD  + PE+ FPLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDAAVSPEVGFPLNPP 60

Query: 61  PSALLLRELLPVVKFSDLPN-PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDH 120
            SA+LLRE LPVVKFSD+P    E CAVCLYEFEGAEEIRWLTNCKHIFHR C+DRWMDH
Sbjct: 61  ASAVLLREFLPVVKFSDVPQAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWMDH 120

Query: 121 DHDTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 163
           D  TCPLCRTPFVPDGMMDEFNQRLWAASG+AE F+++FNSVLG
Sbjct: 121 DQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAE-FDSEFNSVLG 163

BLAST of Pay0014328 vs. ExPASy TrEMBL
Match: A0A6J1HIP9 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC111464396 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.7e-67
Identity = 134/167 (80.24%), Postives = 143/167 (85.63%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSD-NLPPELQFPLNPP 60
           MGFPVGCTEVFFPTIFLHLLSLLDLLK LIL LFRLLGLPEFL SD  +  E   PLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLYSDAAISREGGIPLNPP 60

Query: 61  PSALLLRELLPVVKFSDLPN----PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRW 120
            SA++LRE LPVVKFSD+P       E CAVCLYEFEGAEEIRWLTNCKHIFHR C+DRW
Sbjct: 61  ASAVVLREFLPVVKFSDVPQAAEAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120

Query: 121 MDHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 163
           MDHD  TCPLCRTPFVPDGMMDEFNQRLWAASG+AE F+++FNSVLG
Sbjct: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAE-FDSEFNSVLG 166

BLAST of Pay0014328 vs. NCBI nr
Match: XP_008458180.1 (PREDICTED: E3 ubiquitin-protein ligase RHA1B [Cucumis melo] >TYK03034.1 E3 ubiquitin-protein ligase RHA1B [Cucumis melo var. makuwa])

HSP 1 Score: 342.8 bits (878), Expect = 1.6e-90
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60
           MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120
           SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 163
           DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG
Sbjct: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 162

BLAST of Pay0014328 vs. NCBI nr
Match: XP_004138493.1 (E3 ubiquitin-protein ligase RHA1B [Cucumis sativus])

HSP 1 Score: 334.7 bits (857), Expect = 4.4e-88
Identity = 157/162 (96.91%), Postives = 160/162 (98.77%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60
           MGFPVGCTEVFFPTIFLHLLSLLD+LKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDVLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120
           SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRC+D WMDHDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDSWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 163
           DTCPLCRTPFVPD MMDEFNQRLWAASG+AETFETDFNSVLG
Sbjct: 121 DTCPLCRTPFVPDEMMDEFNQRLWAASGIAETFETDFNSVLG 162

BLAST of Pay0014328 vs. NCBI nr
Match: XP_038875326.1 (probable E3 ubiquitin-protein ligase RHA1A [Benincasa hispida])

HSP 1 Score: 324.3 bits (830), Expect = 5.9e-85
Identity = 152/162 (93.83%), Postives = 157/162 (96.91%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60
           MGFPVGCTEVFFPTIFLHLLS+LD+LK LI SLFRLLGLPEFL SD  PPELQFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSVLDVLKLLIQSLFRLLGLPEFLHSDAFPPELQFPLNPPP 60

Query: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120
           SALLLRELLPVVKFSDLP+PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRC+DRWMDHDH
Sbjct: 61  SALLLRELLPVVKFSDLPDPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 163
           DTCPLCRTPFVPDGMMDEFNQRLWAASG+AETFETDFNSVLG
Sbjct: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVLG 162

BLAST of Pay0014328 vs. NCBI nr
Match: XP_023000136.1 (probable E3 ubiquitin-protein ligase RHA1A [Cucurbita maxima])

HSP 1 Score: 274.6 bits (701), Expect = 5.3e-70
Identity = 137/164 (83.54%), Postives = 146/164 (89.02%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSD-NLPPELQFPLNPP 60
           MGFPVGCTEVFFPTIFLHLLSLLDLLK LIL LFRLLGLPEFL SD  + PE+ FPLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDAAVSPEVGFPLNPP 60

Query: 61  PSALLLRELLPVVKFSDLPN-PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDH 120
            SA+LLRE LPVVKFSD+P    E CAVCLYEFEGAEEIRWLTNCKHIFHR C+DRWMDH
Sbjct: 61  ASAVLLREFLPVVKFSDVPQAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWMDH 120

Query: 121 DHDTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 163
           D  TCPLCRTPFVPDGMMDEFNQRLWAASG+AE F+++FNSVLG
Sbjct: 121 DQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAE-FDSEFNSVLG 163

BLAST of Pay0014328 vs. NCBI nr
Match: KAG6593657.1 (Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025989.1 putative E3 ubiquitin-protein ligase RHA1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 266.5 bits (680), Expect = 1.5e-67
Identity = 134/167 (80.24%), Postives = 144/167 (86.23%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSD-NLPPELQFPLNPP 60
           MGFPVGCTEVFFPTIFLHLLSLLDLLK LIL LFRLLGLPEFL SD  +  E+  PLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDAAVSREVGIPLNPP 60

Query: 61  PSALLLRELLPVVKFSDLPN----PPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRW 120
            SA++LRE LPVVKFSD+P       E CAVCLYEFEGAEEIRWLTNCKHIFHR C+DRW
Sbjct: 61  ASAVVLREFLPVVKFSDVPQAAEAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120

Query: 121 MDHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 163
           MDHD  TCPLCRTPFVPDGMMDEFNQRLWAASG+AE F+++FNSVLG
Sbjct: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAE-FDSEFNSVLG 166

BLAST of Pay0014328 vs. TAIR 10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2 )

HSP 1 Score: 200.3 bits (508), Expect = 1.2e-51
Identity = 98/159 (61.64%), Postives = 116/159 (72.96%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPE-LQFPLNPP 60
           MGFPVG TEVF P +F+  LS+L  ++ ++ S+FR LGL +FL  D   P+   +P   P
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYTSYPTRIP 60

Query: 61  P-----SALLLRELLPVVKFSDLPNP----PERCAVCLYEFEGAEEIRWLTNCKHIFHRR 120
                 SALL+RE+LPV+KF +L N     PE CAVCLYEFEG +EIRWL NC+HIFHR 
Sbjct: 61  ETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRS 120

Query: 121 CLDRWMDHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGV 150
           CLDRWMDHD  TCPLCRTPFVPD M +EFNQRLWAASGV
Sbjct: 121 CLDRWMDHDQKTCPLCRTPFVPDEMQEEFNQRLWAASGV 159

BLAST of Pay0014328 vs. TAIR 10
Match: AT1G63840.1 (RING/U-box superfamily protein )

HSP 1 Score: 160.6 bits (405), Expect = 1.0e-39
Identity = 86/168 (51.19%), Postives = 115/168 (68.45%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60
           MGFPVG +E+  P IF +LLS L L++ LI ++F+++GLP+FL  +  P    +P +PPP
Sbjct: 1   MGFPVGYSELLLPKIFFYLLSFLGLIRKLISTMFKIIGLPDFLEPE--PVSTSWP-DPPP 60

Query: 61  -------SALLLRELLPVVKFSDLPNP-PERCAVCLYEFEGAEEIRWLTNCKHIFHRRCL 120
                  +A+L  E+LPVV+FSD+  P  E CAVCLY+FE  +EIR LTNC+HIFHR CL
Sbjct: 61  TLTKPDSAAILAGEMLPVVRFSDINRPESECCAVCLYDFENDDEIRRLTNCRHIFHRGCL 120

Query: 121 DRW-MDHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNS 160
           DRW M ++  TCPLCRT F+PD +  EFNQRLW+ S    +   D +S
Sbjct: 121 DRWMMGYNQMTCPLCRTQFIPDHLQLEFNQRLWSQSSAVSSQLLDESS 165

BLAST of Pay0014328 vs. TAIR 10
Match: AT5G41400.1 (RING/U-box superfamily protein )

HSP 1 Score: 152.5 bits (384), Expect = 2.9e-37
Identity = 85/169 (50.30%), Postives = 110/169 (65.09%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNL--------PPEL 60
           MG+PVG TE+  P IFLHLLSLL L++ LI + FR+LGLP+FL SD +        PP +
Sbjct: 1   MGYPVGYTELLLPRIFLHLLSLLGLIRTLIDTGFRILGLPDFLESDPVSSSSSWLEPPYM 60

Query: 61  QFPLNP--------PPSALLLRELLPVVKFSDLPNP-----PERCAVCLYEFEGAEEIRW 120
               +         P +A L  E+LPV++FS+L  P      + CAVCL+EFE  +EIR 
Sbjct: 61  STAAHHHQESSFFFPVAARLAGEILPVIRFSELTRPGFGSGSDCCAVCLHEFENDDEIRR 120

Query: 121 LTNCKHIFHRRCLDRW-MDHDHDTCPLCRTPFVPDGMMDEFNQRLWAAS 148
           LTNC+HIFHR CLDRW M ++  TCPLCRTPF+ D +   FNQR+W+ S
Sbjct: 121 LTNCQHIFHRSCLDRWMMGYNQMTCPLCRTPFISDELQVAFNQRVWSES 169

BLAST of Pay0014328 vs. TAIR 10
Match: AT4G11370.1 (RING-H2 finger A1A )

HSP 1 Score: 93.6 bits (231), Expect = 1.6e-19
Identity = 57/151 (37.75%), Postives = 87/151 (57.62%), Query Frame = 0

Query: 1   MGFPVG-CTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSD--NLPPELQFPLN 60
           MG P    TE+  P+  L +L ++   + ++ +L   +GLP FL  +  + P   +  L+
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHNETSAPDLTRHALS 60

Query: 61  PPPSALLLRELLPVVKFSDLPNPPER-CAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRW- 120
              SA L  EL+PVV+FSDLP  PE  C VCL +FE  +++R L  C H+FH  CLDRW 
Sbjct: 61  --TSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYCLDRWI 120

Query: 121 MDHDHDTCPLCRTPFVPDGMMDEFNQRLWAA 147
           +D++   CP+CR  F+P    +++ Q  W +
Sbjct: 121 VDYNKMKCPVCRHRFLP---KEKYTQSDWGS 146

BLAST of Pay0014328 vs. TAIR 10
Match: AT4G11360.1 (RING-H2 finger A1B )

HSP 1 Score: 92.8 bits (229), Expect = 2.7e-19
Identity = 57/149 (38.26%), Postives = 78/149 (52.35%), Query Frame = 0

Query: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60
           MG P    E+  P   L  L ++   + ++ +L   +GLP FL  +         L    
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALST 60

Query: 61  SALLLRELLPVVKFSDLPNPPER-CAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRW-MDH 120
           SA L  EL+PVV+FSDL   PE  C VCL +F   ++IR L  C H+FH RCLDRW +D 
Sbjct: 61  SATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRWIVDC 120

Query: 121 DHDTCPLCRTPFVPDGMMDEFNQRLWAAS 148
           +  TCP+CR  F+P+     F+   W  S
Sbjct: 121 NKITCPICRNRFLPEEKSTPFD---WGTS 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XF921.7e-5061.64Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH... [more]
Q9SUS42.2e-1837.75Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q9SUS53.8e-1838.26E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 ... [more]
Q5EAE95.8e-1145.83RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2[more]
Q6NML02.2e-1046.03E3 ubiquitin-protein ligase ATL76 OS=Arabidopsis thaliana OX=3702 GN=ATL76 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A5D3BV357.7e-91100.00E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3C7827.7e-91100.00E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103497696 PE=4 S... [more]
A0A0A0K8742.1e-8896.91RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G006705 P... [more]
A0A6J1KF162.6e-7083.54probable E3 ubiquitin-protein ligase RHA1A OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1HIP92.7e-6780.24E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
Match NameE-valueIdentityDescription
XP_008458180.11.6e-90100.00PREDICTED: E3 ubiquitin-protein ligase RHA1B [Cucumis melo] >TYK03034.1 E3 ubiqu... [more]
XP_004138493.14.4e-8896.91E3 ubiquitin-protein ligase RHA1B [Cucumis sativus][more]
XP_038875326.15.9e-8593.83probable E3 ubiquitin-protein ligase RHA1A [Benincasa hispida][more]
XP_023000136.15.3e-7083.54probable E3 ubiquitin-protein ligase RHA1A [Cucurbita maxima][more]
KAG6593657.11.5e-6780.24Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
AT3G61460.11.2e-5161.64brassinosteroid-responsive RING-H2 [more]
AT1G63840.11.0e-3951.19RING/U-box superfamily protein [more]
AT5G41400.12.9e-3750.30RING/U-box superfamily protein [more]
AT4G11370.11.6e-1937.75RING-H2 finger A1A [more]
AT4G11360.12.7e-1938.26RING-H2 finger A1B [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 84..126
e-value: 5.3E-6
score: 35.9
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 83..127
e-value: 9.4E-12
score: 45.1
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 84..127
score: 12.371254
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 67..133
e-value: 2.1E-17
score: 64.1
NoneNo IPR availablePANTHERPTHR45969:SF10BRH1 RING FINGER PROTEINcoord: 1..153
NoneNo IPR availablePANTHERPTHR45969RING ZINC FINGER PROTEIN-RELATEDcoord: 1..153
NoneNo IPR availableCDDcd16448RING-H2coord: 84..127
e-value: 5.3871E-14
score: 60.5435
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 70..133

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0014328.1Pay0014328.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061630 ubiquitin protein ligase activity