Pay0013364 (gene) Melon (Payzawat) v1

Overview


Name	Pay0013364
Type	gene
Organism	Cucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description	RING/U-box superfamily protein
Location	chr09: 513469 .. 515687 (+)
RNA-Seq Expression	Pay0013364
Synteny	Pay0013364

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGATGATGATAAAACCACAACTACTTGCAATATCTGTACAGACGACAAAGCCTCGATGAACATGTTCACAAACCACGAGTGCTCCCATTCATTCTGCAAAGATCGCATCTCTAGTCACATCGCAGCCAAATTGGAAGATAACATAGCCAACGTCAAGTGTCCACAGACAGACTGCCAGGCATTGCTACACCCAGATATCTGTCATTCCTTCATCCCCAAAAACTTGTGTTTGATCGCTGGGGAGATGTGTTATGCGAGGCCTTCATTCTAGGGAACCATAGACTCATCTATTGCCCCTTCATGGACTGCTCGGTGGCGCTTATCGATGACGGAGACGAGCCAGCCAAGGAAGCAGAGTGTACACGAGGTGTAATAGAATGTTCTGCGCCAAGTGTAATGTGAAGTGGCATGCGGAAGTAGAATGCGAGGAGTTTCAAAAGCTGTGTTTGGAGGAAAATGGAAGAGATGATTTGTTGGCAATGAAGTTGGCGGAGCAGCAGAATTGGAAGAGATGCCCACATTGTAGGATTTATGTGGAGAAGATCGAGGGTTGCCCTTATGTAATTTGTAGGTGTGGAACTAAATTTTGCTATAGTTGTGGAGCTAAGTGGGGTGGCCGTCATGCATGTCCTGAATCTGCTGAGGCACGGAAGTACCATAAATAAAAAGACAAATACTTGAATGCATTCTCTTAGATTTTAGAAAAAATTGTCAAATTGTAACTACACAATTTGATGGGAGATAAAAAAAAGTTGGATATAATCGTCTAAATACATCTAAATCATATAGCATGAGGTTACTACATGTGTTTGGAAAGCATACCCATCCCTCCACACACACGCATCGAAAGGGTAGCATATATATGAGCTAAAAAGCTCAATGAATTGAATGGCAATGATTAATTTTAGTAATAACCTAACTTTAAAGAAACTTTCCAAGTATAAATCATTAGAATAAATCCACGTAATAATAACCCTCTAGTGACCTTGACATATGAGTTTGTGAAAATTGTTGCTAAATATCTTCACCCCTAACTTATTAGTTATATAATTGGCGGATGATTGATAGCATTGCGTATGGATGGATAGACTTACTTTTTCATTCACTGCAACAAAAAATGAAATTTCATGCATGACCCATGAATAGCAGCTCAAGAATGGATGGGATGTGAAAAATAAGTCATAAAAACTCATTTCATACACTTTTTCCACAAACGAAAAACTTGTCATCAATTTATAATTGAACAAAAAAAGTCATGAAATGTACTAATATGAAAAATGCCCCAAAAGACAGGAAAAAACAACAAAATTTCATAAGTTTCTATATACATATATGAAGGGATTTGATTACAAAGATCATAAAATGAACTTATATATTTTAAATTCAGTATCTATATACATATATAGAGACACAATATCTCAATATCACAATATCTGAATATACATATAAAGGGAAGATTTGAAAATTCAGTATATACAATATGTATGTGTGTGTATATATATATGAACTCATTATCTCAATTTAATTTTGTGCATTATTAGATGGAAAGTATTGCACTGAATTCATGTAATTCGTATTCTATATATTATATAAATATATAAAAAGAAAAAAGATTTACATGTTTATTTATATAAGTTGAAATTACATTATATAATAATACATACAAAAATAGGGTCGTCCTTTATTTCTACTAATCCGAATTTAAAGCCAAATATTTCTTAAAAGATAATAATAACAATATTAAAGCCAAAGAGACGCAATAACTGAGATTAAATGGAAATGCATGCAGCAATTTGCGAGGCAGATTAAAAGAATTTTCCACACTATATATATATTGATCTCTTTTTTTCAATATCCGATGAGGCGATGCAATTAATAATGGAATTGGAAAAGACCCTTTTTCAAATGCCTCTTCATCCTCTCTAGCGTTGTTTAGTATTTAACCCTTGGGGAGAAATGATTAAAAAACCATATAATAGCACACAAAGAATGGAAAGAAAATTGAAAGAAGAATTGATGAGAATGGAAAGAAAATTGAAAGAAGAATTGATGATAGTGAAGTTGGCGGAGAAGATGAAATGGAAAAGATGCCCAAATTGCAAGAATTATGTGGAGAAAATAGAGGGTTGCTCTCATATTACCTGCATATGTGGATCTCAATTTTGCTATACTTGTGGAACTTACTGGACTCAAAACCATGCATGTCTCACATAA

mRNA sequence

ATGGCAGATGATGATAAAACCACAACTACTTGCAATATCTGTACAGACGACAAAGCCTCGATGAACATGTTCACAAACCACGAGTGCTCCCATTCATTCTGCAAAGATCGCATCTCTAGTCACATCGCAGCCAAATTGGAAGATAACATAGCCAACGTCAAGTGTCCACAGACAGACTGCCAGGCATTGCTACACCCAGATATCTGTCATTCCTTCATCCCCAAAAACTTGTGTTTGATCGCTGGGGAGATACGAGCCAGCCAAGGAAGCAGAGTGTACACGAGGTGTAATAGAATGTTCTGCGCCAAGTGTAATGTGAAGTGGCATGCGGAAGTAGAATGCGAGGAGTTTCAAAAGCTGTGTTTGGAGGAAAATGGAAGAGATGATTTGTTGGCAATGAAGTTGGCGGAGCAGCAGAATTGGAAGAGATGCCCACATTGTAGGATTTATGTGGAGAAGATCGAGGGTTGCCCTTATAAAATAGAGGGTTGCTCTCATATTACCTGCATATGTGGATCTCAATTTTGCTATACTTGTGGAACTTACTGGACTCAAAACCATGCATGTCTCACATAA

Coding sequence (CDS)

ATGGCAGATGATGATAAAACCACAACTACTTGCAATATCTGTACAGACGACAAAGCCTCGATGAACATGTTCACAAACCACGAGTGCTCCCATTCATTCTGCAAAGATCGCATCTCTAGTCACATCGCAGCCAAATTGGAAGATAACATAGCCAACGTCAAGTGTCCACAGACAGACTGCCAGGCATTGCTACACCCAGATATCTGTCATTCCTTCATCCCCAAAAACTTGTGTTTGATCGCTGGGGAGATACGAGCCAGCCAAGGAAGCAGAGTGTACACGAGGTGTAATAGAATGTTCTGCGCCAAGTGTAATGTGAAGTGGCATGCGGAAGTAGAATGCGAGGAGTTTCAAAAGCTGTGTTTGGAGGAAAATGGAAGAGATGATTTGTTGGCAATGAAGTTGGCGGAGCAGCAGAATTGGAAGAGATGCCCACATTGTAGGATTTATGTGGAGAAGATCGAGGGTTGCCCTTATAAAATAGAGGGTTGCTCTCATATTACCTGCATATGTGGATCTCAATTTTGCTATACTTGTGGAACTTACTGGACTCAAAACCATGCATGTCTCACATAA

Protein sequence

MADDDKTTTTCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDCQALLHPDICHSFIPKNLCLIAGEIRASQGSRVYTRCNRMFCAKCNVKWHAEVECEEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIYVEKIEGCPYKIEGCSHITCICGSQFCYTCGTYWTQNHACLT

Homology

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BLAST of Pay0013364 vs. ExPASy Swiss-Prot
Match: D3YYI7 (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 3.1e-11
Identity = 54/213 (25.35%), Postives = 92/213 (43.19%), Query Frame = 0


Query: 11  CNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDCQALLHP---- 70
           C +C +DK    +     C  + C++ +  +++++++     +KCP T+C   L      
Sbjct: 236 CRVCLEDKPIKPLPC---CKKAVCEECLKIYLSSQVQLGQVEIKCPVTECFEFLEETTVV 295

Query: 71  -DICH-SFIPKNLCLIAGEIRAS--------------------QGSRVYTR-------CN 130
            ++ H   I     L  G I +S                      SR  +R       C 
Sbjct: 296 YNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESRYKIQCPTCQ 355

Query: 131 RMFCAKCNVKWHAEVECEEFQKLCLEENGRDDLLAMKLAE----QQNWKRCPHCRIYVEK 186
            ++C KC+  WH  V C+E++K        D LL    +E    Q+N ++CP C+I+++ 
Sbjct: 356 LIWCFKCHSPWHEGVNCKEYKK-------GDKLLRHWASEIEHGQRNAQKCPKCKIHIQ- 415

[Close]

BLAST of Pay0013364 vs. ExPASy Swiss-Prot
Match: Q8TC41 (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 69.3 bits (168), Expect = 5.3e-11
Identity = 51/213 (23.94%), Postives = 87/213 (40.85%), Query Frame = 0


Query: 11  CNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDCQALLH----- 70
           C +C +DK    +     C  + C++ +  +++A+++     +KCP T+C   L      
Sbjct: 263 CRVCLEDKPIKPLPC---CKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFLEETTVV 322

Query: 71  ---------------------------PDICHSFIPKNLCLIAGEIRASQGSRVY-TRCN 130
                                      P   H    K    I    R+    ++    C 
Sbjct: 323 YNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYKIQCPTCQ 382

Query: 131 RMFCAKCNVKWHAEVECEEFQKLCLEENGRDDLLAMKLAE----QQNWKRCPHCRIYVEK 186
            ++C KC+  WH  V C+E++K        D LL    +E    Q+N ++CP C+I+++ 
Sbjct: 383 FVWCFKCHSPWHEGVNCKEYKK-------GDKLLRHWASEIEHGQRNAQKCPKCKIHIQ- 442

[Close]

BLAST of Pay0013364 vs. ExPASy Swiss-Prot
Match: Q9US46 (E3 ubiquitin-protein ligase itt1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=itt1 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.9e-09
Identity = 50/233 (21.46%), Postives = 91/233 (39.06%), Query Frame = 0


Query: 11  CNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNI-ANVKCPQTDC--------- 70
           CN+C D+    + F    C H  C+  +  +    +++ + + +KC   DC         
Sbjct: 174 CNVCFDEFNGTDCFQLTRCGHVSCQSCLRDYYTMCIQEGMFSQIKCIDLDCGKDAPVLTL 233

Query: 71  ---QALLHPDICHS-----------------FIPKNLCLIAGEIRASQGSRVYTRCNRMF 130
              ++++   + +                  F P++ C    +    Q   +  +C+  F
Sbjct: 234 KELESIVGVQLTNRYKELEEKRRYENDSNIIFCPRSFCQGPSKRDPGQKLAICQKCDFAF 293

Query: 131 CAKCNVKWHAEVE-CE---EFQKLC-----LEENGRDDLLAM----------KLAEQ--- 183
           C+ C   WH ++  C+   + +KL       +EN  +  L +          +L EQ   
Sbjct: 294 CSFCQATWHGDLSPCKLEGDSKKLVEMYLNYQENEPEKALELEKRYGKRIIDRLVEQVKN 353

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BLAST of Pay0013364 vs. ExPASy Swiss-Prot
Match: Q9LVW9 (Putative E3 ubiquitin-protein ligase ARI4 OS=Arabidopsis thaliana OX=3702 GN=ARI4 PE=5 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 4.9e-09
Identity = 49/214 (22.90%), Postives = 81/214 (37.85%), Query Frame = 0


Query: 6   KTTTTCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKL-EDNIANVKCPQTDCQALL 65
           K T  C+IC ++  S    T  EC H FC D    H   ++ E     ++C    C  + 
Sbjct: 114 KKTMKCDICMEEDLSKYAMTRMECGHRFCNDCWKEHFTVRINEGEGKRIRCMAYKCNTIC 173

Query: 66  HPD---------------ICHSFIPKNLCL--------IAGEIRASQG----SRVYTRCN 125
                             +  S++  N  +            IR  +       V   C 
Sbjct: 174 DEARQLVSTELAEKFDRFLIESYVEDNNMVKWCPSTPHCGNAIRNIKDDGDVDEVECSCG 233

Query: 126 RMFCAKCNVKWHAEVEC---EEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIYVEKI 185
             FC  C  + H+   C   + ++K C +E+   + + +      N K CP C   ++  
Sbjct: 234 LQFCFSCLSESHSPCSCLMWKLWKKKCEDESETVNWMTV------NTKLCPKCSKPIQ-- 293

Query: 186 EGCPYKIEGCSHITCICGSQFCYTCGTYWTQNHA 189
                K +GC+H+TC CG  FC+ CG    ++H+
Sbjct: 294 -----KRDGCNHMTCKCGQHFCWLCGQATGRDHS 314

[Close]

BLAST of Pay0013364 vs. ExPASy Swiss-Prot
Match: A5PK27 (E3 ubiquitin-protein ligase RNF144B OS=Bos taurus OX=9913 GN=RNF144B PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 5.5e-08
Identity = 47/195 (24.10%), Postives = 73/195 (37.44%), Query Frame = 0


Query: 10  TCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIAN-VKCPQTDC-------- 69
           TC +C  +++   M T  EC   FC   +  ++   + +   + + CP   C        
Sbjct: 30  TCKLCLCEQSLDKMTTLQECRCIFCTACLKQYMQLAIREGCGSPITCPDMVCLNHGTLQE 89

Query: 70  --------------------QALLHPDICHSFIPKNLCLIAGEIRASQ-GSRVYTR---C 129
                               +  +H D C ++ P   C     +  S  G  V      C
Sbjct: 90  AEIACLVPVDQFQLYQRLKFEREVHLDPCRTWCPVADCQTVCPVATSDPGQPVLVECPSC 149

Query: 130 NRMFCAKCNVKWHAEVECEEFQKLCL-EENGRDDLLAMKLAEQQNWKRCPHCRIYVEKIE 170
           +  FC+ C   WHAEV C + Q   L  E+G               K+CP CR+Y+E+ E
Sbjct: 150 HLKFCSCCKDAWHAEVSCRDSQPGILPTEHG----TLFGTETDAPIKQCPVCRVYIERNE 209

[Close]

BLAST of Pay0013364 vs. ExPASy TrEMBL
Match: A0A5A7TCD1 (Putative E3 ubiquitin-protein ligase RNF217 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00360 PE=3 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.6e-82
Identity = 156/219 (71.23%), Postives = 163/219 (74.43%), Query Frame = 0


Query: 1   MADDDKTTTTCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDC 60
           MADDDKTTTTCNICTDDKASMNMFTNHECSHSFCKD ISSHIAAKLEDNIANVKCPQTDC
Sbjct: 1   MADDDKTTTTCNICTDDKASMNMFTNHECSHSFCKDCISSHIAAKLEDNIANVKCPQTDC 60

Query: 61  QALLHPDICHSFIPKNL--------------------------CLIA----GEIRASQGS 120
           QALLHPDICHSFIPKNL                          C +A    G+  A +  
Sbjct: 61  QALLHPDICHSFIPKNLFDRWGDVLCEAFILGNHRLIYCPFMDCSVALIDDGDEPAKEAE 120

Query: 121 RVYTRCNRMFCAKCNVKWHAEVECEEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIY 180
              TRCNRMFCAKCNVKWHA VECEEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIY
Sbjct: 121 --CTRCNRMFCAKCNVKWHAGVECEEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIY 180

Query: 181 VEKIEGCPYKIEGCSHITCICGSQFCYTCGTYWTQNHAC 190
           VE IEGCPY       + C CG++FCY+CG  W   HAC
Sbjct: 181 VEMIEGCPY-------VICRCGTKFCYSCGAKWGGRHAC 210

[Close]

BLAST of Pay0013364 vs. ExPASy TrEMBL
Match: A0A0A0KSV5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G650600 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 8.9e-70
Identity = 138/218 (63.30%), Postives = 150/218 (68.81%), Query Frame = 0


Query: 1   MADDDKTTTTCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDC 60
           MADDDKTTT CNICTDDKA +NMFTNHECSHSFCKD IS+HIAAKLEDNIANVKCPQ  C
Sbjct: 1   MADDDKTTTNCNICTDDKALVNMFTNHECSHSFCKDCISNHIAAKLEDNIANVKCPQPGC 60

Query: 61  QALLHPDICHSFIPKN--------LC--LIAGEIR-------------ASQGSRVYTR-- 120
           +A+LHPD+CHSF+PKN        LC   I G  R                G        
Sbjct: 61  EAVLHPDVCHSFVPKNVLDRWGYVLCEAFILGNHRLIYCPFMDCSVALIDDGDEATKEAE 120

Query: 121 ---CNRMFCAKCNVKWHAEVECEEFQKLCLEENGRDD-LLAMKLAEQQNWKRCPHCRIYV 180
              CNRMFCAKCNV WH  VECEEFQKLCLEE  RDD LLA+KLAEQ+NWKRCPHCR YV
Sbjct: 121 CPGCNRMFCAKCNVVWHGGVECEEFQKLCLEEKERDDHLLAIKLAEQENWKRCPHCRTYV 180

Query: 181 EKIEGCPYKIEGCSHITCICGSQFCYTCGTYWTQNHAC 190
           E IEGCPY       I C C ++FCY+CG  W  +HAC
Sbjct: 181 EMIEGCPY-------IICRCRTKFCYSCGAKWGGSHAC 211

[Close]

BLAST of Pay0013364 vs. ExPASy TrEMBL
Match: A0A6J1GX07 (probable E3 ubiquitin-protein ligase ARI10 OS=Cucurbita moschata OX=3662 GN=LOC111457924 PE=3 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 1.3e-44
Identity = 99/210 (47.14%), Postives = 130/210 (61.90%), Query Frame = 0


Query: 7   TTTTCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDCQALLHP 66
           +TT C ICTD K++  MFT+  CSH+FC D IS HIAAKLED++A VKCP+  C A+L P
Sbjct: 70  STTLCTICTDTKSASEMFTSTGCSHTFCADCISKHIAAKLEDSMA-VKCPEPKCAAVLEP 129

Query: 67  DICHSFIPKNLCLIAGE--IRAS--QGSRVY---------------------TRCNRMFC 126
           ++C SF+PK +    G+  ++A   +   VY                      +C RMFC
Sbjct: 130 EMCDSFVPKEVLERWGDALLKAMLLKWKMVYCPFKDCSAGMVDEEDMEAVECPKCWRMFC 189

Query: 127 AKCNVKWHAEVECEEFQKLCLEENG--RDDLLAMKLAEQQNWKRCPHCRIYVEKIEGCPY 186
           AKC V WH ++ C EFQ+L L+  G  R++ + MKLA+++ WKRCPHC+I VE       
Sbjct: 190 AKCGVGWHGDMNCTEFQRL-LKNGGVDREEEMTMKLADERKWKRCPHCKILVE------- 249

Query: 187 KIEGCSHITCICGSQFCYTCGTYWTQNHAC 190
           KIEGC HITC CGS+FCY CG  W  +HAC
Sbjct: 250 KIEGCLHITCRCGSEFCYCCGAKWNGHHAC 270

[Close]

BLAST of Pay0013364 vs. ExPASy TrEMBL
Match: A0A368UHX4 (Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_07G044500 PE=3 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 1.1e-43
Identity = 90/208 (43.27%), Postives = 120/208 (57.69%), Query Frame = 0


Query: 11  CNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDCQALLHPDICH 70
           C IC D K    MF N  CSHSFC D I  ++A K+++NI+ VKCP   C+ ++ P  C 
Sbjct: 90  CGICMDAKYGEEMFRNQNCSHSFCDDCIGRYVATKVQENISMVKCPHPKCKGVIEPQYCR 149

Query: 71  SFIPKN--------LC--LIAGE------------IRASQGSRVYT-----RCNRMFCAK 130
           SFIPK         LC  L+ G             +  +    + T      CNR+FCA+
Sbjct: 150 SFIPKEVFDRWEDALCENLVPGSQKFYCPFKDCSAMLINDAEEIVTVSECPHCNRLFCAQ 209

Query: 131 CNVKWHAEVECEEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIYVEKIEGCPYKIEG 190
           C V WHA VEC+EFQ L   E  R+DL+ M+LA+ +NWKRCP C  YVE       +I+G
Sbjct: 210 CKVSWHAGVECKEFQNLKEYEREREDLMVMELAKNKNWKRCPKCSFYVE-------RIDG 269

Query: 191 CSHITCICGSQFCYTCGTYWTQNHACLT 192
           C+HI+C CG++FCY CG+ W  +HAC+T
Sbjct: 270 CTHISCRCGNEFCYACGSKWGSHHACVT 290

[Close]

BLAST of Pay0013364 vs. ExPASy TrEMBL
Match: A0A6P6GMD9 (probable E3 ubiquitin-protein ligase RNF217 OS=Ziziphus jujuba OX=326968 GN=LOC107429985 PE=3 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 1.1e-43
Identity = 88/213 (41.31%), Postives = 125/213 (58.69%), Query Frame = 0


Query: 4   DDKTTTTCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDCQAL 63
           D+K+   C IC D K+S  MF N  CSHSFC   +  +IA K++ NI+ V+CP   C  +
Sbjct: 60  DEKSHKLCLICMDVKSSARMFRNSSCSHSFCNKCLGKYIATKIQQNISKVECPDLKCNRV 119

Query: 64  LHPDICHSFIPKN--------LC--LIAGE------------IRASQGSRVYTR-----C 123
           L P +C S +PK         LC  L+  E            +    G    T+     C
Sbjct: 120 LEPHLCRSIVPKEVFDKWENALCEALVLEEQKFYCPFKDCSALLVDDGEEAVTQSECPNC 179

Query: 124 NRMFCAKCNVKWHAEVECEEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIYVEKIEG 183
           +R+FCA+C V WH+ +EC++FQ+LC EE GR+D++AM+LA+++ W++CP C++YVE    
Sbjct: 180 HRLFCARCKVSWHSGMECKKFQRLCKEERGREDIMAMELAKKKKWRKCPSCKMYVE---- 239

Query: 184 CPYKIEGCSHITCICGSQFCYTCGTYWTQNHAC 190
              KI GC HITC C  +FCY CG+ W+QNH C
Sbjct: 240 ---KISGCIHITCRCRYEFCYACGSKWSQNHKC 265

[Close]

BLAST of Pay0013364 vs. NCBI nr
Match: KAA0039247.1 (putative E3 ubiquitin-protein ligase RNF217 isoform X2 [Cucumis melo var. makuwa] >TYK00433.1 putative E3 ubiquitin-protein ligase RNF217 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 315.5 bits (807), Expect = 3.2e-82
Identity = 156/219 (71.23%), Postives = 163/219 (74.43%), Query Frame = 0


Query: 1   MADDDKTTTTCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDC 60
           MADDDKTTTTCNICTDDKASMNMFTNHECSHSFCKD ISSHIAAKLEDNIANVKCPQTDC
Sbjct: 1   MADDDKTTTTCNICTDDKASMNMFTNHECSHSFCKDCISSHIAAKLEDNIANVKCPQTDC 60

Query: 61  QALLHPDICHSFIPKNL--------------------------CLIA----GEIRASQGS 120
           QALLHPDICHSFIPKNL                          C +A    G+  A +  
Sbjct: 61  QALLHPDICHSFIPKNLFDRWGDVLCEAFILGNHRLIYCPFMDCSVALIDDGDEPAKEAE 120

Query: 121 RVYTRCNRMFCAKCNVKWHAEVECEEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIY 180
              TRCNRMFCAKCNVKWHA VECEEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIY
Sbjct: 121 --CTRCNRMFCAKCNVKWHAGVECEEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIY 180

Query: 181 VEKIEGCPYKIEGCSHITCICGSQFCYTCGTYWTQNHAC 190
           VE IEGCPY       + C CG++FCY+CG  W   HAC
Sbjct: 181 VEMIEGCPY-------VICRCGTKFCYSCGAKWGGRHAC 210

[Close]

BLAST of Pay0013364 vs. NCBI nr
Match: KAE8649011.1 (hypothetical protein Csa_009107 [Cucumis sativus])

HSP 1 Score: 273.1 bits (697), Expect = 1.8e-69
Identity = 138/218 (63.30%), Postives = 150/218 (68.81%), Query Frame = 0


Query: 1   MADDDKTTTTCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDC 60
           MADDDKTTT CNICTDDKA +NMFTNHECSHSFCKD IS+HIAAKLEDNIANVKCPQ  C
Sbjct: 12  MADDDKTTTNCNICTDDKALVNMFTNHECSHSFCKDCISNHIAAKLEDNIANVKCPQPGC 71

Query: 61  QALLHPDICHSFIPKN--------LC--LIAGEIR-------------ASQGSRVYTR-- 120
           +A+LHPD+CHSF+PKN        LC   I G  R                G        
Sbjct: 72  EAVLHPDVCHSFVPKNVLDRWGYVLCEAFILGNHRLIYCPFMDCSVALIDDGDEATKEAE 131

Query: 121 ---CNRMFCAKCNVKWHAEVECEEFQKLCLEENGRDD-LLAMKLAEQQNWKRCPHCRIYV 180
              CNRMFCAKCNV WH  VECEEFQKLCLEE  RDD LLA+KLAEQ+NWKRCPHCR YV
Sbjct: 132 CPGCNRMFCAKCNVVWHGGVECEEFQKLCLEEKERDDHLLAIKLAEQENWKRCPHCRTYV 191

Query: 181 EKIEGCPYKIEGCSHITCICGSQFCYTCGTYWTQNHAC 190
           E IEGCPY       I C C ++FCY+CG  W  +HAC
Sbjct: 192 EMIEGCPY-------IICRCRTKFCYSCGAKWGGSHAC 222

[Close]

BLAST of Pay0013364 vs. NCBI nr
Match: XP_004141646.1 (probable E3 ubiquitin-protein ligase ARI3 [Cucumis sativus])

HSP 1 Score: 273.1 bits (697), Expect = 1.8e-69
Identity = 138/218 (63.30%), Postives = 150/218 (68.81%), Query Frame = 0


Query: 1   MADDDKTTTTCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDC 60
           MADDDKTTT CNICTDDKA +NMFTNHECSHSFCKD IS+HIAAKLEDNIANVKCPQ  C
Sbjct: 1   MADDDKTTTNCNICTDDKALVNMFTNHECSHSFCKDCISNHIAAKLEDNIANVKCPQPGC 60

Query: 61  QALLHPDICHSFIPKN--------LC--LIAGEIR-------------ASQGSRVYTR-- 120
           +A+LHPD+CHSF+PKN        LC   I G  R                G        
Sbjct: 61  EAVLHPDVCHSFVPKNVLDRWGYVLCEAFILGNHRLIYCPFMDCSVALIDDGDEATKEAE 120

Query: 121 ---CNRMFCAKCNVKWHAEVECEEFQKLCLEENGRDD-LLAMKLAEQQNWKRCPHCRIYV 180
              CNRMFCAKCNV WH  VECEEFQKLCLEE  RDD LLA+KLAEQ+NWKRCPHCR YV
Sbjct: 121 CPGCNRMFCAKCNVVWHGGVECEEFQKLCLEEKERDDHLLAIKLAEQENWKRCPHCRTYV 180

Query: 181 EKIEGCPYKIEGCSHITCICGSQFCYTCGTYWTQNHAC 190
           E IEGCPY       I C C ++FCY+CG  W  +HAC
Sbjct: 181 EMIEGCPY-------IICRCRTKFCYSCGAKWGGSHAC 211

[Close]

BLAST of Pay0013364 vs. NCBI nr
Match: XP_022956145.1 (probable E3 ubiquitin-protein ligase ARI10 [Cucurbita moschata])

HSP 1 Score: 189.5 bits (480), Expect = 2.7e-44
Identity = 99/210 (47.14%), Postives = 130/210 (61.90%), Query Frame = 0


Query: 7   TTTTCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDCQALLHP 66
           +TT C ICTD K++  MFT+  CSH+FC D IS HIAAKLED++A VKCP+  C A+L P
Sbjct: 70  STTLCTICTDTKSASEMFTSTGCSHTFCADCISKHIAAKLEDSMA-VKCPEPKCAAVLEP 129

Query: 67  DICHSFIPKNLCLIAGE--IRAS--QGSRVY---------------------TRCNRMFC 126
           ++C SF+PK +    G+  ++A   +   VY                      +C RMFC
Sbjct: 130 EMCDSFVPKEVLERWGDALLKAMLLKWKMVYCPFKDCSAGMVDEEDMEAVECPKCWRMFC 189

Query: 127 AKCNVKWHAEVECEEFQKLCLEENG--RDDLLAMKLAEQQNWKRCPHCRIYVEKIEGCPY 186
           AKC V WH ++ C EFQ+L L+  G  R++ + MKLA+++ WKRCPHC+I VE       
Sbjct: 190 AKCGVGWHGDMNCTEFQRL-LKNGGVDREEEMTMKLADERKWKRCPHCKILVE------- 249

Query: 187 KIEGCSHITCICGSQFCYTCGTYWTQNHAC 190
           KIEGC HITC CGS+FCY CG  W  +HAC
Sbjct: 250 KIEGCLHITCRCGSEFCYCCGAKWNGHHAC 270

[Close]

BLAST of Pay0013364 vs. NCBI nr
Match: XP_023527456.1 (probable E3 ubiquitin-protein ligase rbrA [Cucurbita pepo subsp. pepo] >XP_023527457.1 probable E3 ubiquitin-protein ligase rbrA [Cucurbita pepo subsp. pepo])

HSP 1 Score: 188.7 bits (478), Expect = 4.6e-44
Identity = 94/208 (45.19%), Postives = 126/208 (60.58%), Query Frame = 0


Query: 9   TTCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDCQALLHPDI 68
           T C ICT  K++  MFT+  CSH+FC D IS HIAA+LED++A VKCP+  C A+L P++
Sbjct: 16  TLCTICTGTKSASEMFTSTGCSHTFCADCISKHIAARLEDSMA-VKCPEPKCAAVLEPEM 75

Query: 69  CHSFIPKNL-------------------------CLIAGEIRASQGSRVYTRCNRMFCAK 128
           C SF+PK +                         CL     +  + +    +C RMFCAK
Sbjct: 76  CDSFVPKEVLERWGDALLKAMLLKWKMVYCPFKDCLAGMVDKEDREAVECPKCWRMFCAK 135

Query: 129 CNVKWHAEVECEEFQKLCLEENG--RDDLLAMKLAEQQNWKRCPHCRIYVEKIEGCPYKI 188
           C V WH ++ C EFQ+L L+  G  R++++ MKLA+++ WKRCPHC+I VE       KI
Sbjct: 136 CGVGWHGDMNCTEFQRL-LKNGGVDREEVMTMKLADERKWKRCPHCKILVE-------KI 195

Query: 189 EGCSHITCICGSQFCYTCGTYWTQNHAC 190
           EGC HITC CGS+FCY+CG  W  +HAC
Sbjct: 196 EGCLHITCRCGSEFCYSCGAKWNGHHAC 214

[Close]

BLAST of Pay0013364 vs. TAIR 10
Match: AT3G53690.1 (RING/U-box superfamily protein )

HSP 1 Score: 156.0 bits (393), Expect = 3.0e-38
Identity = 79/209 (37.80%), Postives = 104/209 (49.76%), Query Frame = 0


Query: 2   ADDDKTTT-TCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDC 61
           +   KT T  C IC D K+ +  F    CSH +C D +S +IAAKL+DNI +++CP + C
Sbjct: 105 SSSSKTATFDCEICVDSKSIIESFRIGGCSHFYCNDCVSKYIAAKLQDNILSIECPVSGC 164

Query: 62  QALLHPDICHSFIPKN--------LC--------------------LIAGEIRASQGSRV 121
              L PD C   +PK         LC                    +   E         
Sbjct: 165 SGRLEPDQCRQILPKEVFDRWGDALCEAVVMRSKKFYCPYKDCSALVFLEESEVKMKDSE 224

Query: 122 YTRCNRMFCAKCNVKWHAEVECEEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIYVE 181
              C+RM C +C  +WH E+ CEEFQKL   E GRDD+L   +A+Q+ WKRCP C+ Y+E
Sbjct: 225 CPHCHRMVCVECGTQWHPEMTCEEFQKLAANERGRDDILLATMAKQKKWKRCPSCKFYIE 284

[Close]

BLAST of Pay0013364 vs. TAIR 10
Match: AT3G14250.1 (RING/U-box superfamily protein )

HSP 1 Score: 147.1 bits (370), Expect = 1.4e-35
Identity = 72/212 (33.96%), Postives = 110/212 (51.89%), Query Frame = 0


Query: 11  CNICTDDKASMNMFT-NHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDCQALLHPDIC 70
           C IC D+K S ++F     C+H++C D    ++A K+++N + +KCP  +C  L+ P  C
Sbjct: 95  CMICMDEKPSSDIFRGTTNCTHAYCTDCTVRYVATKIKENASRIKCPDVECTRLIEPYTC 154

Query: 71  HSFIPKN--------LC----------------LIAGEIRASQGSRVYTR-----CNRMF 130
              IPK+        LC                  A  +    G    T+     C+R+F
Sbjct: 155 RDLIPKDVFDRWEKILCESLISSWDKFYCPFKDCSAMMVNNENGDANVTQTECRSCHRLF 214

Query: 131 CAKCNVKWHAEVECEEFQKL---CLEENGRDDLLAMKLAEQQNWKRCPHCRIYVEKIEGC 190
           C +C V WHA + C+EFQ+      + +  DD L +++A+ + W+RCP C+ YV+     
Sbjct: 215 CVQCKVTWHAGIGCDEFQRFGNTKKKSSDEDDALLIQMAKNKQWRRCPSCKFYVD----- 274

[Close]

BLAST of Pay0013364 vs. TAIR 10
Match: AT4G19670.1 (RING/U-box superfamily protein )

HSP 1 Score: 122.9 bits (307), Expect = 2.9e-28
Identity = 71/230 (30.87%), Postives = 105/230 (45.65%), Query Frame = 0


Query: 3   DDDKTTTTCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDCQA 62
           D DK    C+IC +D+ S  M  + +C+H FC   + +++  K++ +   ++CPQ  C+ 
Sbjct: 199 DGDKPIENCSICCEDRQS-EMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKH 258

Query: 63  LLHPDICHSFIP--------------KN------------------LCLIAGEIRASQGS 122
            L    C SF+P              KN                   CL +G  RAS  S
Sbjct: 259 YLSAAECKSFLPVTTFKSFEEANVCSKNNGKIYCPYPNCSFLLDPQECLSSG--RASSSS 318

Query: 123 RVYTR---------CNRMFCAKCNVKWHAEVECEEFQKLCLEENGRDDLLAMKLAEQQNW 182
              +          C R  C  C V WHA + CEEFQ L ++E   DD+   +LA  + W
Sbjct: 319 STQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKRW 378

Query: 183 KRCPHCRIYVEKIEGCPYKIEGCSHITCICGSQFCYTCGTYWTQNHACLT 192
           +RC  CRI +E         +GC+H+TC CG +FCY+CG  + +     T
Sbjct: 379 RRCQQCRIMIE-------LAQGCNHMTCRCGHEFCYSCGAEYREGQQTCT 418

[Close]

BLAST of Pay0013364 vs. TAIR 10
Match: AT4G19670.2 (RING/U-box superfamily protein )

HSP 1 Score: 122.9 bits (307), Expect = 2.9e-28
Identity = 71/230 (30.87%), Postives = 105/230 (45.65%), Query Frame = 0


Query: 3   DDDKTTTTCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDCQA 62
           D DK    C+IC +D+ S  M  + +C+H FC   + +++  K++ +   ++CPQ  C+ 
Sbjct: 199 DGDKPIENCSICCEDRQS-EMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKH 258

Query: 63  LLHPDICHSFIP--------------KN------------------LCLIAGEIRASQGS 122
            L    C SF+P              KN                   CL +G  RAS  S
Sbjct: 259 YLSAAECKSFLPVTTFKSFEEANVCSKNNGKIYCPYPNCSFLLDPQECLSSG--RASSSS 318

Query: 123 RVYTR---------CNRMFCAKCNVKWHAEVECEEFQKLCLEENGRDDLLAMKLAEQQNW 182
              +          C R  C  C V WHA + CEEFQ L ++E   DD+   +LA  + W
Sbjct: 319 STQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKRW 378

Query: 183 KRCPHCRIYVEKIEGCPYKIEGCSHITCICGSQFCYTCGTYWTQNHACLT 192
           +RC  CRI +E         +GC+H+TC CG +FCY+CG  + +     T
Sbjct: 379 RRCQQCRIMIE-------LAQGCNHMTCRCGHEFCYSCGAEYREGQQTCT 418

[Close]

BLAST of Pay0013364 vs. TAIR 10
Match: AT5G37560.1 (RING/U-box superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 4.4e-21
Identity = 57/209 (27.27%), Postives = 93/209 (44.50%), Query Frame = 0


Query: 10  TCNICTDDKASMNMFTNHECSHSFCKDRISSHIAAKLEDNIANVKCPQTDCQALLH---- 69
           TC+IC+D   + +M  N +C H  C   +   +  KL   I    C +  C++ L     
Sbjct: 180 TCSICSDKTDAEHMLLNDKCLHRHCFSCVKQQVKVKLRSGIV-PPCLEDGCKSELTLESC 239

Query: 70  ---------------------PDICHSFIPKNLC-LIAGEIRASQGS-----RVYTRCNR 129
                                PD    + P   C ++  +   S+ +     R   +C+ 
Sbjct: 240 SMVLTPKLIEMWKRKMEEDLIPDAEKIYCPYRSCSMLMSKTELSREAEQSNVRACIKCSE 299

Query: 130 MFCAKCNVKWHAEVECEEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIYVEKIEGCP 188
           +FC  C V WH+++ C +++++  E    D +L + LA  Q W++C  C+  +E      
Sbjct: 300 LFCIDCKVPWHSDLSCADYKRIHSERLVNDMMLKV-LANDQMWRQCSECKHMIE------ 359

The following BLAST results are available for this feature:

BLAST of Pay0013364 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D3YYI7	3.1e-11	25.35	Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 P...	[more]
Q8TC41	5.3e-11	23.94	Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE...	[more]
Q9US46	2.9e-09	21.46	E3 ubiquitin-protein ligase itt1 OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]
Q9LVW9	4.9e-09	22.90	Putative E3 ubiquitin-protein ligase ARI4 OS=Arabidopsis thaliana OX=3702 GN=ARI...	[more]
A5PK27	5.5e-08	24.10	E3 ubiquitin-protein ligase RNF144B OS=Bos taurus OX=9913 GN=RNF144B PE=2 SV=1	[more]

BLAST of Pay0013364 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TCD1	1.6e-82	71.23	Putative E3 ubiquitin-protein ligase RNF217 isoform X2 OS=Cucumis melo var. maku...	[more]
A0A0A0KSV5	8.9e-70	63.30	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G650600 PE=3 SV=1	[more]
A0A6J1GX07	1.3e-44	47.14	probable E3 ubiquitin-protein ligase ARI10 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A368UHX4	1.1e-43	43.27	Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_07G044500 PE=3 SV=1	[more]
A0A6P6GMD9	1.1e-43	41.31	probable E3 ubiquitin-protein ligase RNF217 OS=Ziziphus jujuba OX=326968 GN=LOC1...	[more]

BLAST of Pay0013364 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0039247.1	3.2e-82	71.23	putative E3 ubiquitin-protein ligase RNF217 isoform X2 [Cucumis melo var. makuwa...	[more]
KAE8649011.1	1.8e-69	63.30	hypothetical protein Csa_009107 [Cucumis sativus]	[more]
XP_004141646.1	1.8e-69	63.30	probable E3 ubiquitin-protein ligase ARI3 [Cucumis sativus]	[more]
XP_022956145.1	2.7e-44	47.14	probable E3 ubiquitin-protein ligase ARI10 [Cucurbita moschata]	[more]
XP_023527456.1	4.6e-44	45.19	probable E3 ubiquitin-protein ligase rbrA [Cucurbita pepo subsp. pepo] >XP_02352...	[more]

BLAST of Pay0013364 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53690.1	3.0e-38	37.80	RING/U-box superfamily protein	[more]
AT3G14250.1	1.4e-35	33.96	RING/U-box superfamily protein	[more]
AT4G19670.1	2.9e-28	30.87	RING/U-box superfamily protein	[more]
AT4G19670.2	2.9e-28	30.87	RING/U-box superfamily protein	[more]
AT5G37560.1	4.4e-21	27.27	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.120.1750		coord: 112..191 e-value: 9.1E-12 score: 47.4
None	No IPR available	PANTHER	PTHR11685:SF350	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 8..183
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 5..77
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 128..190
IPR002867	IBR domain	PFAM	PF01485	IBR	coord: 134..189 e-value: 1.8E-5 score: 24.9
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 5..88 e-value: 6.6E-15 score: 56.8
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 8..183
IPR044066	TRIAD supradomain	PROSITE	PS51873	TRIAD	coord: 7..191 score: 21.391113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Pay0013364.1	Pay0013364.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity