Pay0012984 (gene) Melon (Payzawat) v1

Overview
NamePay0012984
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr11: 32356924 .. 32358728 (+)
RNA-Seq ExpressionPay0012984
SyntenyPay0012984
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ACGAGAGCTTAATGTTGCCTTCAATAATCTACCCAACTATCTCTCTTTCCAATTCCACCGTCTTCAATGGAGGATATTCACCAAGATCCTTTTCCTCCACTACACGGCCCGTCGCCACCACCAACCGCCTCTGTGGACTTTCCGCCACACTTCCGATGCCCAATTTCCATGGAGCTCATGCAAGATCCCGTCACGATATCCACCGGAGTTTCCTTTGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACTATTGTCAACTTTGATATCACACCAAATTATACACTCAAAAGGCTCATACTTTCTTGGAAAATGAAAGAGTCTTGCTCCTCCACGTCGTTGTTGCCATCCTCGTCGATGGAGTATCATGATGTTGTGGCTGATCTCTTGAAGAACATCGAGTCGTCACCGTTCAAGGCGAGTTCTTTGAAGAAACTACGATTGCTCATGGCGGTGGATGATGTAGCTGCTGTCGGAGCGAAACCCGTCTTTGTTCGAGTAAATGGGTTTGAAATTCTTGTCCGAATAGTAACGCAAGTCGCCATTTGTGAGTGCTCCGATTTTGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTGTTGCAAAAACTGATCACACCTCAAAGTTTAAGTTTTTTTTTTCTTTCTTTCTTAACATATAATTTATCGGATTGAAAATATATGTTTTAATTAGTTGAACCTATAATTTATTTTGGTCAAAACATTTAAACTTTATATTTATAATAGAAGAGTTACGTTTGTAGATATCGATATTAATGAGAGATGAGTAATATCACCGAATACATGAGACTAGCTACTGTTAATTAAATTCAAAAGCTTAAATATTTGAAGGGATAGACAGTGATATATTCAATCTTTTATTCATATTCTTAACAGGTATTATCTCAATTTCCGTTTTCCAAAACCGAGAAGCCATTTGAGTTGCTATCGGAACCGGAAGCCATCAAATCAATGGCGATTATCTTACAAAGAGGAAGCATAGAAGGAAGATTTTACGCCATGGAAATGCTCCAACAAATATCCAAAAAGGGCTACGATTGGAACTCTCTAACGAAACATCAAATCATCGACTTATTTAAATCAATATTGGAGCTTGCATCGGATGAGATAATCAAGAAAGCAAGTGCAATCAAGACAATGGATTCCTCGACAATTACACAATGGTACAATATCATCGTCCACACACATATTTTCATTTTATATAGCTTCATTTTAATTGCTAATTAATTGTTTATTTTCTTAGTCGATATGGGACATTGTTCTCATCTGCCCTAGAGCTTTTAATAGAAATCATGGAATCATCGAAAAAAAGTCGTCTTTTATCAATTGAGGCTGGCGCTGTTTGCGTCATGATCGATCTTCTTCCGGACTCGAACCGATCGAAGTGTGAGAAAATTCTATACATACTCAAACTTCTAAGTGAGTGTGCAGAGGGAAGGTCAGCCATGGGGGAACATACTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATATTGTGGTATATTTGTAGTTATCATCCAAGTGAGAGGGTTTTAGAAGAAATGATGGGGTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATGGTGATGGAAGTTCTTCAACCAAGGAGAAGGCCATGAAGGTTATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTATAGGGATTATTTGAAGTTGATAAATCAATAG

mRNA sequence

ACGAGAGCTTAATGTTGCCTTCAATAATCTACCCAACTATCTCTCTTTCCAATTCCACCGTCTTCAATGGAGGATATTCACCAAGATCCTTTTCCTCCACTACACGGCCCGTCGCCACCACCAACCGCCTCTGTGGACTTTCCGCCACACTTCCGATGCCCAATTTCCATGGAGCTCATGCAAGATCCCGTCACGATATCCACCGGAGTTTCCTTTGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACTATTGTCAACTTTGATATCACACCAAATTATACACTCAAAAGGCTCATACTTTCTTGGAAAATGAAAGAGTCTTGCTCCTCCACGTCGTTGTTGCCATCCTCGTCGATGGAGTATCATGATGTTGTGGCTGATCTCTTGAAGAACATCGAGTCGTCACCGTTCAAGGCGAGTTCTTTGAAGAAACTACGATTGCTCATGGCGGTGGATGATGTAGCTGCTGTCGGAGCGAAACCCGTCTTTGTTCGAGTAAATGGGTTTGAAATTCTTGTCCGAATAGTAACGCAAGTCGCCATTTGTGAGTGCTCCGATTTTGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTTCCGTTTTCCAAAACCGAGAAGCCATTTGAGTTGCTATCGGAACCGGAAGCCATCAAATCAATGGCGATTATCTTACAAAGAGGAAGCATAGAAGGAAGATTTTACGCCATGGAAATGCTCCAACAAATATCCAAAAAGGGCTACGATTGGAACTCTCTAACGAAACATCAAATCATCGACTTATTTAAATCAATATTGGAGCTTGCATCGGATGAGATAATCAAGAAAGCAAGTGCAATCAAGACAATGGATTCCTCGACAATTACACAATGTCGATATGGGACATTGTTCTCATCTGCCCTAGAGCTTTTAATAGAAATCATGGAATCATCGAAAAAAAGTCGTCTTTTATCAATTGAGGCTGGCGCTGTTTGCGTCATGATCGATCTTCTTCCGGACTCGAACCGATCGAAGTGTGAGAAAATTCTATACATACTCAAACTTCTAAGTGAGTGTGCAGAGGGAAGGTCAGCCATGGGGGAACATACTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATATTGTGGTATATTTGTAGTTATCATCCAAGTGAGAGGGTTTTAGAAGAAATGATGGGGTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATGGTGATGGAAGTTCTTCAACCAAGGAGAAGGCCATGAAGGTTATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTATAGGGATTATTTGAAGTTGATAAATCAATAG

Coding sequence (CDS)

ATGGAGGATATTCACCAAGATCCTTTTCCTCCACTACACGGCCCGTCGCCACCACCAACCGCCTCTGTGGACTTTCCGCCACACTTCCGATGCCCAATTTCCATGGAGCTCATGCAAGATCCCGTCACGATATCCACCGGAGTTTCCTTTGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACTATTGTCAACTTTGATATCACACCAAATTATACACTCAAAAGGCTCATACTTTCTTGGAAAATGAAAGAGTCTTGCTCCTCCACGTCGTTGTTGCCATCCTCGTCGATGGAGTATCATGATGTTGTGGCTGATCTCTTGAAGAACATCGAGTCGTCACCGTTCAAGGCGAGTTCTTTGAAGAAACTACGATTGCTCATGGCGGTGGATGATGTAGCTGCTGTCGGAGCGAAACCCGTCTTTGTTCGAGTAAATGGGTTTGAAATTCTTGTCCGAATAGTAACGCAAGTCGCCATTTGTGAGTGCTCCGATTTTGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTTCCGTTTTCCAAAACCGAGAAGCCATTTGAGTTGCTATCGGAACCGGAAGCCATCAAATCAATGGCGATTATCTTACAAAGAGGAAGCATAGAAGGAAGATTTTACGCCATGGAAATGCTCCAACAAATATCCAAAAAGGGCTACGATTGGAACTCTCTAACGAAACATCAAATCATCGACTTATTTAAATCAATATTGGAGCTTGCATCGGATGAGATAATCAAGAAAGCAAGTGCAATCAAGACAATGGATTCCTCGACAATTACACAATGTCGATATGGGACATTGTTCTCATCTGCCCTAGAGCTTTTAATAGAAATCATGGAATCATCGAAAAAAAGTCGTCTTTTATCAATTGAGGCTGGCGCTGTTTGCGTCATGATCGATCTTCTTCCGGACTCGAACCGATCGAAGTGTGAGAAAATTCTATACATACTCAAACTTCTAAGTGAGTGTGCAGAGGGAAGGTCAGCCATGGGGGAACATACTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATATTGTGGTATATTTGTAGTTATCATCCAAGTGAGAGGGTTTTAGAAGAAATGATGGGGTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATGGTGATGGAAGTTCTTCAACCAAGGAGAAGGCCATGAAGGTTATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTATAGGGATTATTTGAAGTTGATAAATCAATAG

Protein sequence

MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ
Homology
BLAST of Pay0012984 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 3.1e-46
Identity = 131/429 (30.54%), Postives = 213/429 (49.65%), Query Frame = 0

Query: 23  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 82
           ++ PP F CPIS+E+M+DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 83  YTLKRLILSWKMKESCSSTSLLPS-----SSMEYHDVVADLLKNIESSPFKASSLKKLRL 142
           +TL+RLI SW    +      +P+        E   ++ D   + E+   +   LK+LR 
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHEN---QVKCLKRLRQ 129

Query: 143 LMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANFEACEEALGVLSQFPFSK 202
           +++ +       K         E L  IV+       +D  N    +EAL +L     S+
Sbjct: 130 IVSEN----ATNKRCLEAAGVPEFLANIVS-------NDSENGSLTDEALNLLYHLETSE 189

Query: 203 T--EKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLF 262
           T  +       +   +KS+  I+QRG  E R YA  +L+ I +      S+T     ++F
Sbjct: 190 TVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVF 249

Query: 263 KSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEIMESSKKSRLLSIEAG 322
             ++++  D I +KA+                    +A+ +L+ I     ++R  ++EAG
Sbjct: 250 TEVVQILDDRISQKAT-------------------KAAMHILVNICPWG-RNRHKAVEAG 309

Query: 323 AVCVMIDLLPD----SNRSKCEKILYILKLLSECAEGRSAMGEHTMGIATVTKK-MSISN 382
            + V+I+LL D    S R   E  + +L LL +CAEGR+    H   IA V KK + +S 
Sbjct: 310 VISVIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQ 369

Query: 383 TATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEKAMKVMKLHGS 440
           TA+   +++L  +  +  +  +L EM+  G V KL  +L       TKEKA +++KLH  
Sbjct: 370 TASDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHAR 402

BLAST of Pay0012984 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 4.0e-41
Identity = 128/453 (28.26%), Postives = 220/453 (48.57%), Query Frame = 0

Query: 23  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 82
           ++ P  F CPIS+++M+DPV +STG++++R +IEKW F+  K +CP T Q I   D+TPN
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPN 64

Query: 83  YTLKRLILSWKMKESCSSTSLLPSSSMEY-HDVVADLLKNIESSPF-KASSLKKLRLLMA 142
           +TL+RLI SW    +      +P+         +  L+K   SS   +   LK+LR +++
Sbjct: 65  HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVS 124

Query: 143 VDDVAAVGAKPVFVRVNGFEILVRIVTQVA-----------------ICECSDFAN-FEA 202
            +       K         E L  IV+                    +C+ +   N F++
Sbjct: 125 ENTT----NKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 184

Query: 203 ----CEEALGVLSQFPFSKTEKPFELLSE--PEAIKSMAIILQRGSIEGRFYAMEMLQQI 262
                +EAL VL     S+T     L ++     +K++  I+QRG  E R YA  +L+++
Sbjct: 185 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 244

Query: 263 SKKGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALEL 322
            +       +   +  +LF  ++++  D+I  KA+                    SA+++
Sbjct: 245 LEVADPMQIILLER--ELFGEVIQILHDQISHKAT-------------------RSAMQI 304

Query: 323 LIEIMESSKKSRLLSIEAGAVCVMIDLLPD----SNRSKCEKILYILKLLSECAEGRSAM 382
           L+ I     ++R  ++E G + ++I+LL D    S R   E  + +L +L +CAEGR+  
Sbjct: 305 LV-ITCPWGRNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEF 364

Query: 383 GEHTMGIATVTKK-MSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNG 442
             H   IA V+KK + +S   ++  +++L  +  +  +  +L+EM+  G V KL  +L  
Sbjct: 365 LNHGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQV 424

Query: 443 DGSSSTKEKAMKVMKLHGSFWRRFPCFPYEYRD 445
              + TKEKA +++KLH   WR  PC P    D
Sbjct: 425 SCGNKTKEKAKELLKLHARVWRESPCVPRNLYD 431

BLAST of Pay0012984 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 5.6e-35
Identity = 123/433 (28.41%), Postives = 202/433 (46.65%), Query Frame = 0

Query: 18  PPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNF 77
           P    +  P HFRCPIS+ELMQDPVT+ TG +++RA+IE W    N  TCP T   + +F
Sbjct: 7   PLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDF 66

Query: 78  DITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDV-VADLLKNIE-------SSPFKA 137
            + PN+TL+RLI  W +    +    +P+         V  LL           S   +A
Sbjct: 67  TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRA 126

Query: 138 SSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANFEACEEALGV 197
           ++L++LR      D   V    +    N  EIL++I+        S+  + E   E+L +
Sbjct: 127 AALRRLRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELVSESLAL 186

Query: 198 LSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQIS--KKGYDWNSLT 257
           L   P ++  +   + S+P  ++ +  +L   SIE R  A  +++ +S   K  D     
Sbjct: 187 LVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSI 246

Query: 258 KHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEIMESSKKS 317
            +    +F+ +L+L  + I  +  A+K    +    C                  S K +
Sbjct: 247 SNS-ESVFEGVLDLLRNPISSR-RALKIGIKTLFALC------------------SVKST 306

Query: 318 RLLSIEAGAVCVMID-LLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIATVTKK- 377
           R ++I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  
Sbjct: 307 RHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTI 366

Query: 378 MSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEKAMKVM 437
           + +S+ AT+     L  +C+    ER  EE  G G V +LL ++  + +   K+KA K++
Sbjct: 367 LRVSDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLL 406

Query: 438 KLHGSFWRRFPCF 439
           KL    W  +  F
Sbjct: 427 KLLRDSWPDYNSF 406

BLAST of Pay0012984 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.3e-33
Identity = 115/440 (26.14%), Postives = 209/440 (47.50%), Query Frame = 0

Query: 23  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTI-VNFDITP 82
           ++ P +F CPIS+E+M+DPVT  +G++++R NI KW       +CP T Q + ++ D+TP
Sbjct: 8   IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTP 67

Query: 83  NYTLKRLILSWKMKESCS-----STSLLPSSSMEYHDVVADLLKNIESSPFKASSLKKLR 142
           N+ L+RLI  W ++         ST  +P   +   + + +L K  + +  +  +L+KL 
Sbjct: 68  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 127

Query: 143 LLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANFEACEEALGVLSQFPFS 202
           +L A+D       + +       + L+  V +    +       +  +E+L +L      
Sbjct: 128 VL-AMDG----NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIP 187

Query: 203 KTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLFK 262
             +    L+     ++S+  +L +     + Y + +L+ +++  Y  + + +    ++FK
Sbjct: 188 SNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFK 247

Query: 263 SILELASDEI----------------IKKASAIKTMDSSTITQCRYGTLFSSALELLIEI 322
            I+    D +                  + S  KT  S            ++AL +L+E 
Sbjct: 248 GIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE- 307

Query: 323 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSK--CEKILYILKLLSECAEGRSAMGEHTMG 382
             S  ++R L ++ GAV  +I+L       K   E +L +L  L  CA GR+ +  H  G
Sbjct: 308 TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGG 367

Query: 383 IATVTKK-MSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSST 438
           IA VTK+ + +S  A    + IL  +  + P   V+EEM+  GTV+KL  +L  D   + 
Sbjct: 368 IAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGLNL 427

BLAST of Pay0012984 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 5.4e-30
Identity = 125/429 (29.14%), Postives = 202/429 (47.09%), Query Frame = 0

Query: 14  GPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQT 73
           G   P    +  P HFRCPIS++LM DPVTISTG +++R +I+ W    N  TCP T   
Sbjct: 3   GNLEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVA 62

Query: 74  IVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDV-VADLLKNIE-------SS 133
           + +F + PN+TL+RLI  W +    +    +P+       + V  LL           S 
Sbjct: 63  LSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSV 122

Query: 134 PFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANFEACEE 193
             +A+++++LR L    +   V    +    N  EILVRI+   A  E +  ++ E   E
Sbjct: 123 RSRAAAIRRLRGLARDSEKNRV----LIAGHNAREILVRIL--FADIETTSLSS-ELVSE 182

Query: 194 ALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKGYDWNS 253
           +L +L     ++TE    + S+P  +  M  +L   SIE R  A  +++ +         
Sbjct: 183 SLALLVLLHMTETECE-AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMV--------- 242

Query: 254 LTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEIMESSK 313
           LT  + +DL   ++   SD I +    +     S+    + G     AL L+       K
Sbjct: 243 LTGAKSMDL--KLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLV-------K 302

Query: 314 KSRLLSIEAGAVCVMID-LLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIATVTK 373
           ++R L+I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K
Sbjct: 303 QTRHLAISAGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVK 362

Query: 374 K-MSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEKAMK 433
             + +S+ AT+     L  +C+    ER  +E    G V +LL ++  D +   K KA  
Sbjct: 363 TILRVSDRATEYAAGALLALCT--AEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQM 402

BLAST of Pay0012984 vs. ExPASy TrEMBL
Match: A0A1S3CC54 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 SV=1)

HSP 1 Score: 894.4 bits (2310), Expect = 1.9e-256
Identity = 451/451 (100.00%), Postives = 451/451 (100.00%), Query Frame = 0

Query: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180
           NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240
           EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEI 300
           YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIA 360
           MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIA
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIA 360

Query: 361 TVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 420
           TVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK
Sbjct: 361 TVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 420

Query: 421 AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ
Sbjct: 421 AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

BLAST of Pay0012984 vs. ExPASy TrEMBL
Match: A0A0A0KBP6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE=4 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 5.8e-237
Identity = 426/454 (93.83%), Postives = 436/454 (96.04%), Query Frame = 0

Query: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQ+PFPP H P+P    +VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQNPFPPPHAPTP---TTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK-ESCSST-SLLPSSSMEYHDVVADL 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK +SCSST SLLPSSSMEYHDVVADL
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADL 120

Query: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFA 180
           LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFE+L+ IVTQVAICE SDFA
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFA 180

Query: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAI+LQRGSIEGRFYAMEMLQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLI 300
           KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKT DSS +T CRYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMG 360
           EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKIL+ILKLLSECAEGRSAMGEH MG
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGIL-NGDGSSST 420
           IATVTKKMSISNTATKIGMKILW IC+YHPSERVLEEMMGCGTVKKLLGIL NGDG SST
Sbjct: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSST 420

Query: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           KEKA KVMKLHGSFWRRFPCFPYEYRDYLKLINQ
Sbjct: 421 KEKAKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

BLAST of Pay0012984 vs. ExPASy TrEMBL
Match: A0A5D3DLY2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00740 PE=4 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 3.8e-228
Identity = 412/451 (91.35%), Postives = 413/451 (91.57%), Query Frame = 0

Query: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180
           NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDF NF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFTNF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240
           EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEI 300
           YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKT DSSTITQCRYGTLFSSALELLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSTITQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIA 360
           MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEG            
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEG------------ 360

Query: 361 TVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 420
                                   SYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK
Sbjct: 361 ------------------------SYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 415

Query: 421 AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           AMKVMKLHGSFWR+FPCFPYEYRDYLKLINQ
Sbjct: 421 AMKVMKLHGSFWRKFPCFPYEYRDYLKLINQ 415

BLAST of Pay0012984 vs. ExPASy TrEMBL
Match: A0A6J1H6I8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075 PE=4 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 5.7e-208
Identity = 377/453 (83.22%), Postives = 403/453 (88.96%), Query Frame = 0

Query: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDI Q+  PP   P PPP    D PP+FRCPISME+M DPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIRQNRLPPPQ-PLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLP--SSSMEYHDVVADL 120
           TYNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ESCSS S       SMEY DVVA L
Sbjct: 61  TYNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPRPPSMEYDDVVA-L 120

Query: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFA 180
           LKNIE SPFKASSLKKLRLLMA+DDV AVGAKP+FV+ NGFE+LV +VTQ+AICE S+FA
Sbjct: 121 LKNIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFA 180

Query: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVLSQFPFSK EK FELL+ PEAIKSMAI+LQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLI 300
           KGYD+NSL+KH  IDLFKSILELASDEIIKKAS +K  DSS  T CRYGTLFSSALELLI
Sbjct: 241 KGYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKIL++LKLLS+CAEGR AM EH MG
Sbjct: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDG-SSST 420
           IATVTKKMS SNTATKIG+KILW IC+YHPSERVLEEM+GCGTVKKL+GILNGDG SSST
Sbjct: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGILNGDGRSSST 420

Query: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLIN 451
           KEKAMK+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 KEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

BLAST of Pay0012984 vs. ExPASy TrEMBL
Match: A0A6J1KWQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 PE=4 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 1.8e-206
Identity = 378/453 (83.44%), Postives = 405/453 (89.40%), Query Frame = 0

Query: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDI Q+  PP     P P A+ D PP+FRCPISME+M DPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIRQNRLPP-----PQPPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCS--STSLLPSSSMEYHDVVADL 120
           TYNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ESCS  S+S     S+EY DVVA L
Sbjct: 61  TYNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVA-L 120

Query: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFA 180
           LKNIE SPFKASSLKKLRLLMA+DDVAAV AK +FV+ NGFE+LV +VTQVAICE S+FA
Sbjct: 121 LKNIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFA 180

Query: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVLSQFPFSKTEK FELLS+ EAIKSM I+LQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLI 300
           KGYD+NSL+KH  IDLFKSILELASDEIIKKAS IKT DSS  TQCRYGTLFSSALELLI
Sbjct: 241 KGYDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMG 360
           EIMESSKK RLL IEAGA+C+MIDLLPDSNRSKCEKIL++LKLLS+CAEGR AM EH MG
Sbjct: 301 EIMESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDG-SSST 420
           IATVTKKMS SNTATKIG+KILW IC+YHPSERVLEEMMGCGTVKKL+GILNGDG SSST
Sbjct: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSST 420

Query: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLIN 451
           KEKAMK+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 KEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 447

BLAST of Pay0012984 vs. NCBI nr
Match: XP_008460073.1 (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo])

HSP 1 Score: 894.4 bits (2310), Expect = 4.0e-256
Identity = 451/451 (100.00%), Postives = 451/451 (100.00%), Query Frame = 0

Query: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180
           NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240
           EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEI 300
           YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIA 360
           MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIA
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIA 360

Query: 361 TVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 420
           TVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK
Sbjct: 361 TVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 420

Query: 421 AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ
Sbjct: 421 AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

BLAST of Pay0012984 vs. NCBI nr
Match: XP_004145072.2 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical protein Csa_004999 [Cucumis sativus])

HSP 1 Score: 829.7 bits (2142), Expect = 1.2e-236
Identity = 426/454 (93.83%), Postives = 436/454 (96.04%), Query Frame = 0

Query: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQ+PFPP H P+P    +VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQNPFPPPHAPTP---TTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK-ESCSST-SLLPSSSMEYHDVVADL 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK +SCSST SLLPSSSMEYHDVVADL
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADL 120

Query: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFA 180
           LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFE+L+ IVTQVAICE SDFA
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFA 180

Query: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAI+LQRGSIEGRFYAMEMLQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLI 300
           KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKT DSS +T CRYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMG 360
           EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKIL+ILKLLSECAEGRSAMGEH MG
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGIL-NGDGSSST 420
           IATVTKKMSISNTATKIGMKILW IC+YHPSERVLEEMMGCGTVKKLLGIL NGDG SST
Sbjct: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSST 420

Query: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           KEKA KVMKLHGSFWRRFPCFPYEYRDYLKLINQ
Sbjct: 421 KEKAKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

BLAST of Pay0012984 vs. NCBI nr
Match: KAA0039970.1 (E3 ubiquitin-protein ligase PUB23-like [Cucumis melo var. makuwa] >TYK24532.1 E3 ubiquitin-protein ligase PUB23-like [Cucumis melo var. makuwa])

HSP 1 Score: 800.4 bits (2066), Expect = 7.8e-228
Identity = 412/451 (91.35%), Postives = 413/451 (91.57%), Query Frame = 0

Query: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180
           NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDF NF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFTNF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240
           EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEI 300
           YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKT DSSTITQCRYGTLFSSALELLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSTITQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIA 360
           MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEG            
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEG------------ 360

Query: 361 TVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 420
                                   SYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK
Sbjct: 361 ------------------------SYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 415

Query: 421 AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           AMKVMKLHGSFWR+FPCFPYEYRDYLKLINQ
Sbjct: 421 AMKVMKLHGSFWRKFPCFPYEYRDYLKLINQ 415

BLAST of Pay0012984 vs. NCBI nr
Match: XP_038875803.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 798.1 bits (2060), Expect = 3.9e-227
Identity = 406/451 (90.02%), Postives = 423/451 (93.79%), Query Frame = 0

Query: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQ+  PP   P PP    VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQNLLPPPQ-PPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWK KES SS+S  PSSSM+Y DVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDVVADLLK 120

Query: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180
           NIES+PFKASSLKKLRLLMA+DDVAAVGAKPVFVR NGF++LV I+TQVAICE +DFANF
Sbjct: 121 NIESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240
           EACEEALGVLSQFPFSKTEKPFELLS PEAIKSMAI+LQRGS+EGRFYAMEMLQQISKK 
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKE 240

Query: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEI 300
           YDWNSLTKH IIDLFKSILELASDEIIKKAS IKT + S ITQCRYGTLFSSALELLIEI
Sbjct: 241 YDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIA 360
           MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKIL+ILKLLSECAEGRSAMGEH MGIA
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIA 360

Query: 361 TVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 420
           TVTKKMSISNTATKIGMKILW IC+YHPSERVLEEMMGCGTVKKLLGILNGDG SSTKEK
Sbjct: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEK 420

Query: 421 AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 452
           AMK+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 AMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 450

BLAST of Pay0012984 vs. NCBI nr
Match: XP_023549278.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 746.1 bits (1925), Expect = 1.7e-211
Identity = 385/453 (84.99%), Postives = 407/453 (89.85%), Query Frame = 0

Query: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDI Q+  PP   P PPP A  D PP+FRCPISME+M DPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIRQNRLPPSQ-PLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSS--SMEYHDVVADL 120
           TYNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ESCSS S       SMEY DVVA L
Sbjct: 61  TYNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVA-L 120

Query: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFA 180
           LKNIE SPFKASSLKKLRLLMA+DDVAAVGAKP+FV+ NGFE+LV +VTQVAICE S+FA
Sbjct: 121 LKNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFA 180

Query: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240
           NFEACEEALGVLSQFPFSKTEK FELLS PEAIKSMAI+LQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240

Query: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLI 300
           KGYD+NSLTKH  IDLFKSILELASDEIIKKAS +K  DSS  TQCRYGTLFSSALELLI
Sbjct: 241 KGYDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKIL++LKLLS+CAEGR AM EH MG
Sbjct: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360

Query: 361 IATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDG-SSST 420
           IATVTKKMS SNTATKIG+KILW IC+YHPSERVLEEMMGCGTVKKL+GILNGDG SSST
Sbjct: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSST 420

Query: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLIN 451
           KEKAMK+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 KEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

BLAST of Pay0012984 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 187.6 bits (475), Expect = 2.2e-47
Identity = 131/429 (30.54%), Postives = 213/429 (49.65%), Query Frame = 0

Query: 23  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 82
           ++ PP F CPIS+E+M+DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 83  YTLKRLILSWKMKESCSSTSLLPS-----SSMEYHDVVADLLKNIESSPFKASSLKKLRL 142
           +TL+RLI SW    +      +P+        E   ++ D   + E+   +   LK+LR 
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHEN---QVKCLKRLRQ 129

Query: 143 LMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANFEACEEALGVLSQFPFSK 202
           +++ +       K         E L  IV+       +D  N    +EAL +L     S+
Sbjct: 130 IVSEN----ATNKRCLEAAGVPEFLANIVS-------NDSENGSLTDEALNLLYHLETSE 189

Query: 203 T--EKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLF 262
           T  +       +   +KS+  I+QRG  E R YA  +L+ I +      S+T     ++F
Sbjct: 190 TVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVF 249

Query: 263 KSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEIMESSKKSRLLSIEAG 322
             ++++  D I +KA+                    +A+ +L+ I     ++R  ++EAG
Sbjct: 250 TEVVQILDDRISQKAT-------------------KAAMHILVNICPWG-RNRHKAVEAG 309

Query: 323 AVCVMIDLLPD----SNRSKCEKILYILKLLSECAEGRSAMGEHTMGIATVTKK-MSISN 382
            + V+I+LL D    S R   E  + +L LL +CAEGR+    H   IA V KK + +S 
Sbjct: 310 VISVIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQ 369

Query: 383 TATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEKAMKVMKLHGS 440
           TA+   +++L  +  +  +  +L EM+  G V KL  +L       TKEKA +++KLH  
Sbjct: 370 TASDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHAR 402

BLAST of Pay0012984 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 170.6 bits (431), Expect = 2.8e-42
Identity = 128/453 (28.26%), Postives = 220/453 (48.57%), Query Frame = 0

Query: 23  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 82
           ++ P  F CPIS+++M+DPV +STG++++R +IEKW F+  K +CP T Q I   D+TPN
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPN 64

Query: 83  YTLKRLILSWKMKESCSSTSLLPSSSMEY-HDVVADLLKNIESSPF-KASSLKKLRLLMA 142
           +TL+RLI SW    +      +P+         +  L+K   SS   +   LK+LR +++
Sbjct: 65  HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVS 124

Query: 143 VDDVAAVGAKPVFVRVNGFEILVRIVTQVA-----------------ICECSDFAN-FEA 202
            +       K         E L  IV+                    +C+ +   N F++
Sbjct: 125 ENTT----NKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 184

Query: 203 ----CEEALGVLSQFPFSKTEKPFELLSE--PEAIKSMAIILQRGSIEGRFYAMEMLQQI 262
                +EAL VL     S+T     L ++     +K++  I+QRG  E R YA  +L+++
Sbjct: 185 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 244

Query: 263 SKKGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALEL 322
            +       +   +  +LF  ++++  D+I  KA+                    SA+++
Sbjct: 245 LEVADPMQIILLER--ELFGEVIQILHDQISHKAT-------------------RSAMQI 304

Query: 323 LIEIMESSKKSRLLSIEAGAVCVMIDLLPD----SNRSKCEKILYILKLLSECAEGRSAM 382
           L+ I     ++R  ++E G + ++I+LL D    S R   E  + +L +L +CAEGR+  
Sbjct: 305 LV-ITCPWGRNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEF 364

Query: 383 GEHTMGIATVTKK-MSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNG 442
             H   IA V+KK + +S   ++  +++L  +  +  +  +L+EM+  G V KL  +L  
Sbjct: 365 LNHGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQV 424

Query: 443 DGSSSTKEKAMKVMKLHGSFWRRFPCFPYEYRD 445
              + TKEKA +++KLH   WR  PC P    D
Sbjct: 425 SCGNKTKEKAKELLKLHARVWRESPCVPRNLYD 431

BLAST of Pay0012984 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 150.2 bits (378), Expect = 3.9e-36
Identity = 123/433 (28.41%), Postives = 202/433 (46.65%), Query Frame = 0

Query: 18  PPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNF 77
           P    +  P HFRCPIS+ELMQDPVT+ TG +++RA+IE W    N  TCP T   + +F
Sbjct: 7   PLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDF 66

Query: 78  DITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDV-VADLLKNIE-------SSPFKA 137
            + PN+TL+RLI  W +    +    +P+         V  LL           S   +A
Sbjct: 67  TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRA 126

Query: 138 SSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANFEACEEALGV 197
           ++L++LR      D   V    +    N  EIL++I+        S+  + E   E+L +
Sbjct: 127 AALRRLRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELVSESLAL 186

Query: 198 LSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQIS--KKGYDWNSLT 257
           L   P ++  +   + S+P  ++ +  +L   SIE R  A  +++ +S   K  D     
Sbjct: 187 LVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSI 246

Query: 258 KHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEIMESSKKS 317
            +    +F+ +L+L  + I  +  A+K    +    C                  S K +
Sbjct: 247 SNS-ESVFEGVLDLLRNPISSR-RALKIGIKTLFALC------------------SVKST 306

Query: 318 RLLSIEAGAVCVMID-LLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIATVTKK- 377
           R ++I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  
Sbjct: 307 RHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTI 366

Query: 378 MSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEKAMKVM 437
           + +S+ AT+     L  +C+    ER  EE  G G V +LL ++  + +   K+KA K++
Sbjct: 367 LRVSDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLL 406

Query: 438 KLHGSFWRRFPCF 439
           KL    W  +  F
Sbjct: 427 KLLRDSWPDYNSF 406

BLAST of Pay0012984 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 144.8 bits (364), Expect = 1.7e-34
Identity = 115/440 (26.14%), Postives = 209/440 (47.50%), Query Frame = 0

Query: 23  VDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTI-VNFDITP 82
           ++ P +F CPIS+E+M+DPVT  +G++++R NI KW       +CP T Q + ++ D+TP
Sbjct: 22  IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTP 81

Query: 83  NYTLKRLILSWKMKESCS-----STSLLPSSSMEYHDVVADLLKNIESSPFKASSLKKLR 142
           N+ L+RLI  W ++         ST  +P   +   + + +L K  + +  +  +L+KL 
Sbjct: 82  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 141

Query: 143 LLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANFEACEEALGVLSQFPFS 202
           +L A+D       + +       + L+  V +    +       +  +E+L +L      
Sbjct: 142 VL-AMDG----NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIP 201

Query: 203 KTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHQIIDLFK 262
             +    L+     ++S+  +L +     + Y + +L+ +++  Y  + + +    ++FK
Sbjct: 202 SNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFK 261

Query: 263 SILELASDEI----------------IKKASAIKTMDSSTITQCRYGTLFSSALELLIEI 322
            I+    D +                  + S  KT  S            ++AL +L+E 
Sbjct: 262 GIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE- 321

Query: 323 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSK--CEKILYILKLLSECAEGRSAMGEHTMG 382
             S  ++R L ++ GAV  +I+L       K   E +L +L  L  CA GR+ +  H  G
Sbjct: 322 TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGG 381

Query: 383 IATVTKK-MSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSST 438
           IA VTK+ + +S  A    + IL  +  + P   V+EEM+  GTV+KL  +L  D   + 
Sbjct: 382 IAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGLNL 441

BLAST of Pay0012984 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 133.7 bits (335), Expect = 3.8e-31
Identity = 125/429 (29.14%), Postives = 202/429 (47.09%), Query Frame = 0

Query: 14  GPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQT 73
           G   P    +  P HFRCPIS++LM DPVTISTG +++R +I+ W    N  TCP T   
Sbjct: 3   GNLEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVA 62

Query: 74  IVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDV-VADLLKNIE-------SS 133
           + +F + PN+TL+RLI  W +    +    +P+       + V  LL           S 
Sbjct: 63  LSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSV 122

Query: 134 PFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANFEACEE 193
             +A+++++LR L    +   V    +    N  EILVRI+   A  E +  ++ E   E
Sbjct: 123 RSRAAAIRRLRGLARDSEKNRV----LIAGHNAREILVRIL--FADIETTSLSS-ELVSE 182

Query: 194 ALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKGYDWNS 253
           +L +L     ++TE    + S+P  +  M  +L   SIE R  A  +++ +         
Sbjct: 183 SLALLVLLHMTETECE-AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMV--------- 242

Query: 254 LTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEIMESSK 313
           LT  + +DL   ++   SD I +    +     S+    + G     AL L+       K
Sbjct: 243 LTGAKSMDL--KLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLV-------K 302

Query: 314 KSRLLSIEAGAVCVMID-LLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIATVTK 373
           ++R L+I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K
Sbjct: 303 QTRHLAISAGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVK 362

Query: 374 K-MSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEKAMK 433
             + +S+ AT+     L  +C+    ER  +E    G V +LL ++  D +   K KA  
Sbjct: 363 TILRVSDRATEYAAGALLALCT--AEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQM 402

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84TG33.1e-4630.54E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC64.0e-4128.26E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9LT795.6e-3528.41U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9SF152.3e-3326.14E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9FXA45.4e-3029.14U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
A0A1S3CC541.9e-256100.00RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 ... [more]
A0A0A0KBP65.8e-23793.83RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE... [more]
A0A5D3DLY23.8e-22891.35RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1H6I85.7e-20883.22RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075... [more]
A0A6J1KWQ81.8e-20683.44RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 P... [more]
Match NameE-valueIdentityDescription
XP_008460073.14.0e-256100.00PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo][more]
XP_004145072.21.2e-23693.83E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical pro... [more]
KAA0039970.17.8e-22891.35E3 ubiquitin-protein ligase PUB23-like [Cucumis melo var. makuwa] >TYK24532.1 E3... [more]
XP_038875803.13.9e-22790.02E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida][more]
XP_023549278.11.7e-21184.99E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G35930.12.2e-4730.54plant U-box 23 [more]
AT3G52450.12.8e-4228.26plant U-box 22 [more]
AT3G19380.13.9e-3628.41plant U-box 25 [more]
AT3G11840.11.7e-3426.14plant U-box 24 [more]
AT1G49780.13.8e-3129.14plant U-box 26 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 28..92
e-value: 8.6E-24
score: 95.0
IPR003613U box domainPFAMPF04564U-boxcoord: 26..96
e-value: 5.4E-16
score: 58.5
IPR003613U box domainPROSITEPS51698U_BOXcoord: 24..99
score: 27.051916
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 18..102
e-value: 1.7E-23
score: 84.4
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 103..430
e-value: 6.0E-10
score: 40.3
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 24..450
NoneNo IPR availablePANTHERPTHR22849:SF23RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 24..450
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 29..72
e-value: 1.09778E-20
score: 82.6667
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 20..97
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 179..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0012984.1Pay0012984.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity