Pay0011919 (gene) Melon (Payzawat) v1

Overview
NamePay0011919
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionCalcium-binding EF-hand family protein
Locationchr03: 7687821 .. 7688671 (-)
RNA-Seq ExpressionPay0011919
SyntenyPay0011919
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACAATAATTCCACACATATTATATATTTTGGTAATAATCTTTCTTGTGTGTTTTGCATTTCAATTCTAAACCGAAGAACAAAAAGCACCATGTCTCCAATTAGCTCCAAAGATTTGCATAGGATCTTCAAGAAGCTAGACAAGAACTGTGATGGCCTCCTCTGCCTAGAAGAGCTCAAGTGGCTCTTGGATAGGATTGGCATCCAATTAACCATGGAGGAGCTGGAATCATTTCTAGAGAAACCCAGCCTCGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCAAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGGGGGGTGTGTGCAAGATGATCATGAGGATGATACTGAGATTGTATATATGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGGTGAGGCTCGAGCTTTGGGATGCAAGTAGAAGCGAGGTAGATTATTGTAGAAGCATGATCCAAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAGTTCTTCTTTTAGAGTTTCAAAAAAGAAAAGGAAAAAAAAAAAAAAAATCCCCTTTTTTGTTATATTGTTGTTAAATATAGATATTCGTTTGGTCTGTTTATCTGCAGGTACAATTTGAGAAGCTGGGAGGCTTCAAGAATAAAAATTTTGGTCATGGAGTTCATGTAATTATATTTTTGTTGCTCCAATCAGATTATGTATTTCCACTCTCCTCTATAATAGAATTTTTTTTTAAGGATCAATGACTATGGTCTAATTCTGTGTCTTTGTATATG

mRNA sequence

AACAATAATTCCACACATATTATATATTTTGGTAATAATCTTTCTTGTGTGTTTTGCATTTCAATTCTAAACCGAAGAACAAAAAGCACCATGTCTCCAATTAGCTCCAAAGATTTGCATAGGATCTTCAAGAAGCTAGACAAGAACTGTGATGGCCTCCTCTGCCTAGAAGAGCTCAAGTGGCTCTTGGATAGGATTGGCATCCAATTAACCATGGAGGAGCTGGAATCATTTCTAGAGAAACCCAGCCTCGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCAAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGGGGGGTGTGTGCAAGATGATCATGAGGATGATACTGAGATTGTATATATGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGGTGAGGCTCGAGCTTTGGGATGCAAGTAGAAGCGAGGTAGATTATTGTAGAAGCATGATCCAAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAGTTCTTCTTTTAGAGTTTCAAAAAAGAAAAGGAAAAAAAAAAAAAAAATCCCCTTTTTTGTTATATTGTTGTTAAATATAGATATTCGTTTGGTCTGTTTATCTGCAGGTACAATTTGAGAAGCTGGGAGGCTTCAAGAATAAAAATTTTGGTCATGGAGTTCATGTAATTATATTTTTGTTGCTCCAATCAGATTATGTATTTCCACTCTCCTCTATAATAGAATTTTTTTTTAAGGATCAATGACTATGGTCTAATTCTGTGTCTTTGTATATG

Coding sequence (CDS)

ATGTCTCCAATTAGCTCCAAAGATTTGCATAGGATCTTCAAGAAGCTAGACAAGAACTGTGATGGCCTCCTCTGCCTAGAAGAGCTCAAGTGGCTCTTGGATAGGATTGGCATCCAATTAACCATGGAGGAGCTGGAATCATTTCTAGAGAAACCCAGCCTCGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCAAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGGGGGGTGTGTGCAAGATGATCATGAGGATGATACTGAGATTGTATATATGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGGTGAGGCTCGAGCTTTGGGATGCAAGTAGAAGCGAGGTAGATTATTGTAGAAGCATGATCCAAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAG

Protein sequence

MSPISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFFYESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT
Homology
BLAST of Pay0011919 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 128.3 bits (321), Expect = 8.1e-29
Identity = 74/156 (47.44%), Postives = 100/156 (64.10%), Query Frame = 0

Query: 4   ISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIG-IQLTMEELESFLEKPSLDFDEFLFF 63
           I++ DL R+FK LDKN DGL+ L+EL W+LD++G  + T +ELE  + K SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 123
           Y      +KG  K        D   D+ E +  AF VFD+NGDG+IS +EL +VL RL  
Sbjct: 66  YYDAVLDSKGSKKN------IDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGF 125

Query: 124 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMML 159
            + +++    C  MI+ +D NLDG +DFEEFKNM+L
Sbjct: 126 EEEAKAW--DCGRMIRVHDKNLDGFVDFEEFKNMIL 153

BLAST of Pay0011919 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 6.2e-21
Identity = 62/155 (40.00%), Postives = 87/155 (56.13%), Query Frame = 0

Query: 9   LHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFL------EKPSLDFDEFLFF 68
           LHR+F   DKN DG + +EEL   L R+G+     +L+S +      +K  L FD+F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 69  YESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 128
           ++++ +       GG G C     E D E    AF VFD +GDGFIS  EL+ VL +L L
Sbjct: 89  HKTLDE----SFFGGEGSCCDGSPESDLE---EAFNVFDEDGDGFISAVELQKVLKKLGL 148

Query: 129 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
            +A   E++    MI + D+N DG++DF EFKNMM
Sbjct: 149 PEA--GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

BLAST of Pay0011919 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 4.4e-19
Identity = 60/156 (38.46%), Postives = 85/156 (54.49%), Query Frame = 0

Query: 9   LHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLE------KPSLDFDEFLFF 68
           L RIF   DKN DG + +EEL   L R+G+   + +L+S +E         L+FD+F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YESISKQ-NKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLE 128
           ++++      G C GG          ++   +  AFKVFD NGDGFIS  EL+ VL +L 
Sbjct: 90  HKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKLG 149

Query: 129 LWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
           L +    E++    MI + D N DG++DF EFKNMM
Sbjct: 150 LPEG--GEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

BLAST of Pay0011919 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 3.8e-18
Identity = 58/164 (35.37%), Postives = 93/164 (56.71%), Query Frame = 0

Query: 9   LHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLE------KPSLDFDEFLFF 68
           L RIF   DKN DG++ ++EL   L+ +G++  + ELES ++         L F++F+  
Sbjct: 41  LRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELESTVKSFTREGNIGLQFEDFISL 100

Query: 69  YESISKQNKGECKGGVGGCVQDDHEDDTEIVYMA---------FKVFDMNGDGFISCDEL 128
           ++S++     +     GG  +DD+E+D     ++         FKVFD +GDG+IS  EL
Sbjct: 101 HQSLN-----DSYFAYGGEDEDDNEEDMRKSILSQEEADSFGGFKVFDEDGDGYISAREL 160

Query: 129 ENVLVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
           + VL +L    +  SE+D    MI + D+N DG++DF EFK+MM
Sbjct: 161 QMVLGKLGF--SEGSEIDRVEKMIVSVDSNRDGRVDFFEFKDMM 197

BLAST of Pay0011919 vs. ExPASy Swiss-Prot
Match: Q2QVI1 (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 3.8e-18
Identity = 62/168 (36.90%), Postives = 86/168 (51.19%), Query Frame = 0

Query: 4   ISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEK------PSLDFD 63
           + S +L ++FK  DKN DG +  +EL       GI +  +EL++ ++K        +D +
Sbjct: 1   MDSTELRKVFKMFDKNGDGRITKKELGESFKNFGIFIPDDELDATMDKIDANGDGCVDVE 60

Query: 64  EFLFFYESI--------SKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFIS 123
           EF   Y SI        + +      GG GG   D+ E   E    AF VFD NGDGFI+
Sbjct: 61  EFGLLYRSILGDDAAGRAPRTAAAAIGGEGGAPDDEDEGMRE----AFNVFDQNGDGFIT 120

Query: 124 CDELENVLVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
            DEL +VL  L L     +  D CR MI   D + DG++DF+EFK MM
Sbjct: 121 VDELRSVLSSLGLKHGRTA--DDCRRMISMVDADGDGRVDFKEFKQMM 162

BLAST of Pay0011919 vs. ExPASy TrEMBL
Match: A0A5D3B6U1 (Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold282G00080 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 4.0e-87
Identity = 159/161 (98.76%), Postives = 160/161 (99.38%), Query Frame = 0

Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           MSPISSKDLHRIFKKLDKNCDGLLCL+ELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120
           FFYESISKQNKGECKGGV GCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120

Query: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
           ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

BLAST of Pay0011919 vs. ExPASy TrEMBL
Match: A0A1S3B3T2 (probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 4.0e-87
Identity = 159/161 (98.76%), Postives = 160/161 (99.38%), Query Frame = 0

Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           MSPISSKDLHRIFKKLDKNCDGLLCL+ELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120
           FFYESISKQNKGECKGGV GCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120

Query: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
           ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

BLAST of Pay0011919 vs. ExPASy TrEMBL
Match: A0A0A0KFQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 8.8e-79
Identity = 146/165 (88.48%), Postives = 155/165 (93.94%), Query Frame = 0

Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           M PISSKDLHRIFKKLDKNCDGL+CL+EL WLLD IGIQLTMEELESFLE+PSLDFDEFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVGGCVQDDH----EDDTEIVYMAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGV GCVQD+     +DD EIVY+AFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
           LV+LELWDASRS+VDYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

BLAST of Pay0011919 vs. ExPASy TrEMBL
Match: A0A6J1FLU5 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111445399 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.6e-66
Identity = 130/158 (82.28%), Postives = 141/158 (89.24%), Query Frame = 0

Query: 3   PISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62
           PISS DL RIF +LDKN DGL+C++ELKWLLD IGIQLTMEELESFLEKPSLDFDEFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 122
           YESISKQNKGECKGG+      D E+D EIVYMAFKVFD+NGDGFISC+ELENVL RLEL
Sbjct: 64  YESISKQNKGECKGGLVD-EHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 161
           WDA++SEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 124 WDANKSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

BLAST of Pay0011919 vs. ExPASy TrEMBL
Match: A0A6J1K1Y7 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111489388 PE=4 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.9e-65
Identity = 131/161 (81.37%), Postives = 142/161 (88.20%), Query Frame = 0

Query: 3   PISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62
           PISS DL RIF +LDKN DGL+C++ELKWLLD IGIQLTMEELESFLEKPSLDFDEFL F
Sbjct: 4   PISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLIF 63

Query: 63  YESISKQNKGECKGGVGGCVQDDHED---DTEIVYMAFKVFDMNGDGFISCDELENVLVR 122
           YES+SKQNKGECKGG    + D+HED   D EIVYMAFKVFD+NGDGFISC+ELENVL R
Sbjct: 64  YESMSKQNKGECKGG----LVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSR 123

Query: 123 LELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 161
           LELWDA+RSEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 124 LELWDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

BLAST of Pay0011919 vs. NCBI nr
Match: XP_008441608.1 (PREDICTED: probable calcium-binding protein CML44 [Cucumis melo] >TYJ95710.1 putative calcium-binding protein CML44 [Cucumis melo var. makuwa])

HSP 1 Score: 330.5 bits (846), Expect = 8.2e-87
Identity = 159/161 (98.76%), Postives = 160/161 (99.38%), Query Frame = 0

Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           MSPISSKDLHRIFKKLDKNCDGLLCL+ELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120
           FFYESISKQNKGECKGGV GCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120

Query: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
           ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

BLAST of Pay0011919 vs. NCBI nr
Match: XP_004149438.1 (probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 hypothetical protein Csa_018262 [Cucumis sativus])

HSP 1 Score: 302.8 bits (774), Expect = 1.8e-78
Identity = 146/165 (88.48%), Postives = 155/165 (93.94%), Query Frame = 0

Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           M PISSKDLHRIFKKLDKNCDGL+CL+EL WLLD IGIQLTMEELESFLE+PSLDFDEFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVGGCVQDDH----EDDTEIVYMAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGV GCVQD+     +DD EIVY+AFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
           LV+LELWDASRS+VDYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

BLAST of Pay0011919 vs. NCBI nr
Match: XP_038885742.1 (probable calcium-binding protein CML44 [Benincasa hispida])

HSP 1 Score: 293.5 bits (750), Expect = 1.1e-75
Identity = 141/161 (87.58%), Postives = 152/161 (94.41%), Query Frame = 0

Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           MS IS  DLHRIF KLDKN DGL+CL+ELKWL DRIGIQLTMEELESFLEKPSLDF+EFL
Sbjct: 11  MSSISFNDLHRIFNKLDKNGDGLICLQELKWLFDRIGIQLTMEELESFLEKPSLDFNEFL 70

Query: 61  FFYESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120
           FFYESISKQNKGECKGGV GC+QDDHE++ EIVYMAFKVFDMNGDGFISCDELENVL RL
Sbjct: 71  FFYESISKQNKGECKGGV-GCLQDDHEEEMEIVYMAFKVFDMNGDGFISCDELENVLSRL 130

Query: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
           ELWDA+R+++DYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 131 ELWDANRNDIDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 170

BLAST of Pay0011919 vs. NCBI nr
Match: XP_023551499.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 261.2 bits (666), Expect = 6.1e-66
Identity = 131/158 (82.91%), Postives = 141/158 (89.24%), Query Frame = 0

Query: 3   PISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62
           PISS DL RIF +LDKN DGL+C++ELKWLLD IGIQLTMEELESFLEKPSLDFDEFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 122
           YESISKQNKGECKGG+      D E+D EIVYMAFKVFD+NGDGFISC+ELENVL RLEL
Sbjct: 64  YESISKQNKGECKGGLVD-EHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 161
           WDA+RSEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 124 WDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

BLAST of Pay0011919 vs. NCBI nr
Match: XP_022939513.1 (probable calcium-binding protein CML44 [Cucurbita moschata] >KAG7016457.1 putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 260.0 bits (663), Expect = 1.4e-65
Identity = 130/158 (82.28%), Postives = 141/158 (89.24%), Query Frame = 0

Query: 3   PISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62
           PISS DL RIF +LDKN DGL+C++ELKWLLD IGIQLTMEELESFLEKPSLDFDEFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 122
           YESISKQNKGECKGG+      D E+D EIVYMAFKVFD+NGDGFISC+ELENVL RLEL
Sbjct: 64  YESISKQNKGECKGGLVD-EHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 161
           WDA++SEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 124 WDANKSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

BLAST of Pay0011919 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 128.3 bits (321), Expect = 5.7e-30
Identity = 74/156 (47.44%), Postives = 100/156 (64.10%), Query Frame = 0

Query: 4   ISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIG-IQLTMEELESFLEKPSLDFDEFLFF 63
           I++ DL R+FK LDKN DGL+ L+EL W+LD++G  + T +ELE  + K SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 123
           Y      +KG  K        D   D+ E +  AF VFD+NGDG+IS +EL +VL RL  
Sbjct: 66  YYDAVLDSKGSKKN------IDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGF 125

Query: 124 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMML 159
            + +++    C  MI+ +D NLDG +DFEEFKNM+L
Sbjct: 126 EEEAKAW--DCGRMIRVHDKNLDGFVDFEEFKNMIL 153

BLAST of Pay0011919 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 102.1 bits (253), Expect = 4.4e-22
Identity = 62/155 (40.00%), Postives = 87/155 (56.13%), Query Frame = 0

Query: 9   LHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFL------EKPSLDFDEFLFF 68
           LHR+F   DKN DG + +EEL   L R+G+     +L+S +      +K  L FD+F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 69  YESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 128
           ++++ +       GG G C     E D E    AF VFD +GDGFIS  EL+ VL +L L
Sbjct: 89  HKTLDE----SFFGGEGSCCDGSPESDLE---EAFNVFDEDGDGFISAVELQKVLKKLGL 148

Query: 129 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
            +A   E++    MI + D+N DG++DF EFKNMM
Sbjct: 149 PEA--GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

BLAST of Pay0011919 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 95.9 bits (237), Expect = 3.2e-20
Identity = 60/156 (38.46%), Postives = 85/156 (54.49%), Query Frame = 0

Query: 9   LHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLE------KPSLDFDEFLFF 68
           L RIF   DKN DG + +EEL   L R+G+   + +L+S +E         L+FD+F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YESISKQ-NKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLE 128
           ++++      G C GG          ++   +  AFKVFD NGDGFIS  EL+ VL +L 
Sbjct: 90  HKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKLG 149

Query: 129 LWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
           L +    E++    MI + D N DG++DF EFKNMM
Sbjct: 150 LPEG--GEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

BLAST of Pay0011919 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 85.5 bits (210), Expect = 4.3e-17
Identity = 54/156 (34.62%), Postives = 83/156 (53.21%), Query Frame = 0

Query: 8   DLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEK------PSLDFDEFLF 67
           +L R+F+  DKN DG +  +EL   L  +GI +  +EL   +EK        +D DEF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 68  FYESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLE 127
            Y++I                 D+ +++ E +  AF VFD NGDGFI+ DEL+ VL  L 
Sbjct: 65  LYKTI----------------MDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLG 124

Query: 128 LWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
           L       +D C+ MI+  D + DG+++++EF+ MM
Sbjct: 125 L--KQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMM 142

BLAST of Pay0011919 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 84.0 bits (206), Expect = 1.2e-16
Identity = 53/160 (33.12%), Postives = 83/160 (51.88%), Query Frame = 0

Query: 4   ISSKDLHRIFKKLDKNCDGLLCLEELKWLLDRIGIQLTMEELESFLEK------PSLDFD 63
           I   +L R+F+  DKN DG +  EEL   L+ +GI +  ++L   + K        +D D
Sbjct: 61  IDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDID 120

Query: 64  EFLFFYESISKQNKGECKGGVGGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVL 123
           EF   Y SI  ++             +D E + E +  AF VFD +GDGFI+ +EL++V+
Sbjct: 121 EFESLYSSIVDEH------------HNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVM 180

Query: 124 VRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
             L L       +D C+ MI   D + DG+++++EF  MM
Sbjct: 181 ASLGL--KQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMM 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LPK58.1e-2947.44Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ... [more]
Q9FI196.2e-2140.00Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ... [more]
Q9SVG94.4e-1938.46Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1[more]
P431873.8e-1835.37Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1[more]
Q2QVI13.8e-1836.90Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
A0A5D3B6U14.0e-8798.76Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B3T24.0e-8798.76probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 P... [more]
A0A0A0KFQ68.8e-7988.48Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1[more]
A0A6J1FLU56.6e-6682.28probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1K1Y71.9e-6581.37probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114893... [more]
Match NameE-valueIdentityDescription
XP_008441608.18.2e-8798.76PREDICTED: probable calcium-binding protein CML44 [Cucumis melo] >TYJ95710.1 put... [more]
XP_004149438.11.8e-7888.48probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 hypothetica... [more]
XP_038885742.11.1e-7587.58probable calcium-binding protein CML44 [Benincasa hispida][more]
XP_023551499.16.1e-6682.91probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo][more]
XP_022939513.11.4e-6582.28probable calcium-binding protein CML44 [Cucurbita moschata] >KAG7016457.1 putati... [more]
Match NameE-valueIdentityDescription
AT1G21550.15.7e-3047.44Calcium-binding EF-hand family protein [more]
AT5G44460.14.4e-2240.00calmodulin like 43 [more]
AT4G20780.13.2e-2038.46calmodulin like 42 [more]
AT1G05990.14.3e-1734.62EF hand calcium-binding protein family [more]
AT2G43290.11.2e-1633.13Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 132..160
e-value: 0.01
score: 25.0
coord: 8..36
e-value: 0.007
score: 25.6
coord: 92..120
e-value: 0.037
score: 23.2
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 94..157
e-value: 2.3E-10
score: 40.7
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 12..30
e-value: 0.018
score: 14.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 128..161
score: 13.592857
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 4..39
score: 12.170192
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 88..123
score: 12.365459
IPR002048EF-hand domainCDDcd00051EFhcoord: 96..157
e-value: 6.31264E-12
score: 55.6317
IPR002048EF-hand domainCDDcd00051EFhcoord: 8..60
e-value: 2.32099E-7
score: 43.6905
NoneNo IPR availableGENE3D1.10.238.10coord: 77..157
e-value: 4.2E-19
score: 70.7
NoneNo IPR availableGENE3D1.10.238.10coord: 5..69
e-value: 7.1E-9
score: 37.6
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 1..160
NoneNo IPR availablePANTHERPTHR10891:SF788CALCIUM-BINDING PROTEIN CML44-RELATEDcoord: 1..160
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 101..113
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 17..29
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 5..158

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0011919.1Pay0011919.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding